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Development of ecological indicator guilds for land management

Agency land-use must be efficiently and cost-effectively monitored to assess conditions and trends in ecosystem processes and natural resources relevant to mission requirements and legal mandates. Ecological Indicators represent important land management tools for tracking ecological changes and preventing irreversible environmental damage in disturbed landscapes. The overall objective of the research was to develop both individual and integrated sets (i.e., statistically derived guilds) of Ecological Indicators to: quantify habitat conditions and trends, track and monitor ecological changes, provide early warning or threshold detection, and provide guidance for land managers. The derivation of Ecological Indicators was based on statistical criteria, ecosystem relevance, reliability and robustness, economy and ease of use for land managers, multi-scale performance, and stress response criteria. The basis for the development of statistically based Ecological Indicators was the identification of ecosystem metrics that analytically tracked a landscape disturbance gradient.

Report

Using assemblage data in ecological indicators: A comparison and evaluation of commonly available statistical tools

Ecological indicators are science-based tools used to assess how human activities have impacted environmental resources. For monitoring and environmental assessment, existing species assemblage data can be used to make these comparisons through time or across sites. An impediment to using assemblage data, however, is that these data are complex and need to be simplified in an ecologically meaningful way. Because multivariate statistics are mathematical relationships, statistical groupings may not make ecological sense and will not have utility as indicators. Our goal was to define a process to select defensible and ecologically interpretable statistical simplifications of assemblage data in which researchers and managers can have confidence. For this, we chose a suite of statistical methods, compared the groupings that resulted from these analyses, identified convergence among groupings, then we interpreted the groupings using species and ecological guilds. When we tested this approach using a statewide stream fish dataset, not all statistical methods worked equally well. For our dataset, logistic regression (Log), detrended correspondence analysis (DCA), cluster analysis (CL), and non-metric multidimensional scaling (NMDS) provided consistent, simplified output. Specifically, the Log, DCA, CL-1, and NMDS-1 groupings were ≥60% similar to each other, overlapped with the fluvial-specialist ecological guild, and contained a common subset of species. Groupings based on number of species (e.g., Log, DCA, CL and NMDS) outperformed groupings based on abundance [e.g., principal components analysis (PCA) and Poisson regression]. Although the specific methods that worked on our test dataset have generality, here we are advocating a process (e.g., identifying convergent groupings with redundant species composition that are ecologically interpretable) rather than the automatic use of any single statistical tool. We summarize this process in step-by-step guidance for the future use of these commonly available ecological and statistical methods in preparing assemblage data for use in ecological indicators.

Ecological Indicators

Using ecological indicators and a decision support system for integrated ecological assessment at two national park units in the Mid-Atlantic region, U.S.A.

We implemented an integrated ecological assessment using a GIS-based decision support system model for Upper Delaware Scenic and Recreational River (UPDE) and Delaware Water Gap National Recreation Area (DEWA)—national park units with the mid-Atlantic region of the United States. Our assessment examined a variety of aquatic and terrestrial indicators of ecosystem components that reflect the parks’ conservation purpose and reference condition. Our assessment compared these indicators to ecological thresholds to determine the condition of park watersheds. Selected indicators included chemical and physical measures of water quality, biologic indicators of water quality, and landscape condition measures. For the chemical and physical measures of water quality, we used a water quality index and each of its nine components to assess the condition of water quality in each watershed. For biologic measures of water quality, we used the Ephemeroptera, Plecoptera, Trichoptera aquatic macroinvertebrate index and, secondarily, the Hilsenhoff aquatic macroinvertebrate index. Finally, for the landscape condition measures of our model, we used percent forest and percent impervious surface. Based on our overall assessment, UPDE and DEWA watersheds had an ecological assessment score of 0.433 on a −1 to 1 fuzzy logic scale. This score indicates that, in general, the natural resource condition within watersheds at these parks is healthy or ecologically unimpaired; however, we had only partial data for many of our indicators. Our model is iterative and new data may be incorporated as they become available. These natural parks are located within a rapidly urbanizing landscape—we recommend that natural resource managers remain vigilant to surrounding land uses that may adversely affect natural resources within the parks.

New Jersey, New York, Pennsylvania

Environmental setting, water quality, and ecological indicators of surface-water quality in the Mermentau River Basin, southwestern Louisiana, 1998-2001

The U.S. Geological Survey collected data from 29 wells and 24 surface-water sites in the Mermentau River Basin, 1998-2001, to better understand ground-water and surface-water quality; aquatic invertebrate communities; and habitat conditions, in relation to land use. This study was apart of the National Water-Quality Assessment Program, which was designed to assess water quality as it relates to various land uses. Water-quality data were evaluated with criteria established for the protection of drinking water and aquatic life, and bed-sediment data were compared to aquatic life criteria. Water-quality and ecological data were analyzed statistically in relation to drainage area and agricultural land-use integrity. Concentrations of nutrients and major inorganic ions in ground water and surface water generally were highest in the southeastern part of the study area where soils contain thick loess deposits. Peak concentrations of nutrients in surface water occurred March-may at two sites with high agricultural intensity; the lowest concentrations occurred August-January. The greatest potential for eutrophic conditions in surface water, based on nutrient concentrations, existed March-May, at about the same time or shortly after ricefields were drained. Secondary Maximum Contaminant Levels established by the U.S. Environmental Protection Agency (USEPA) were exceeded for sulfate, chloride, iron, or manganese in samples from 20 wells, and for iron or manganese in samples from all surface-water sites. Fewer pesticides were detected in ground water than in surface water. In 11 of of the 29 wells sampled, at least one pesticide or pesticide degradation product was detected. The most frequently detected pesticides or pesticide degradation products in ground water were the herbicides benzaton and atrazine. Concentrations of 47 pesticides and degradation products were detected in surface water. At least 3 pesticides were detected in all surface-water samples. In 72 percent of the samples at least 5 hydrophilic pesticides were detected, and in more than 70 percent of the samples at least 3 hydrophobic pesticides were detected. Although atrazine concentrations in three samples collected in the spring exceeded 3 micrograms per liter, the USEPA Maximum Contaminant Level of 3 micrograms per liter was not exceeded because it is based on an annual average of quarterly samples. Concentrations larger than 3.0 micrograms per liter were not detected in samples collected during other times of the year. Tebuthiuron was detected at all surface-water sites; the largest concentration (6.33 micrograms per liter) was detected at a site on Bayou des Cannes, and was the only detection that exceeded the criterion (1.6 micrograms per liter) for the protection of aquatic life. Malathion was detected at 16 surface-water sites; the largest concentration (0.113 micrograms per liter) was detected at a site on Bayou Lacassine and was the only detection that exceeded the criterion (0.1 micrograms per liter) for the protection of aquatic life. Concentrations of fipronil exceeded numeric targets for acute total maximum daily loads (2.3 micrograms per liter) at 3 sites and chronic total maximum daily loads (4.6 micrograms per liter) at 14 sites. Maximum pesticide concentrations in surface water usually occurred in the spring at about the same time or shortly after ricefields were drained. Concentrations of DDE in bed sediment at two sites exceeded interim freshwater sediment quality guidelines for the protection of aquatic life. Fipronil sulfide and desulfinylpronil were detected at all 17 sites from which bed-sediment samples were collected, but there are no current (2002) guidelines with which to evaluate the environmental effects of fipronil and degradation products. Two methods were used to group the ecological data-collection sites: (1) Sites were grouped before data collection (according to the study design) using drainage area

Water-Resources Investigations Report

Ecological indicators of water quality in the Spokane River, Idaho and Washington, 1998 and 1999

A water-quality investigation of the Spokane River was completed during summer low-flow conditions in 1998 and 1999 as part of the USGS NAWQA Program, in cooperation with the WDOE. (Abbreviations used in this report are defined on the last page.) Samples for analyses of water chemistry; bed sediment; aquatic communities (fish, macroinvertebrates, and algae); contaminants in tissue (fish and macroinvertebrates); and associated measures of habitat were collected at six sites downstream from Coeur d’Alene Lake between river miles 63 and 100. These data provided baseline information to evaluate the water-quality status of the Spokane River and can be used to determine the ecological risk to aquatic organisms from contaminants.

Washington;Idaho

Ecological health indicators

Contemporary policymakers rarely stress ecological knowledge, and yet this knowledge remains crucial—just as it was in prehistory—to protecting overall human well-being. Measuring carefully selected ecological health indicators—that is, signs or symptoms, especially those focused on biotic assemblages—can provide insights into the ecological condition of a place and the variety of ecological consequences of proposed or present human actions there. In turn, measured ecological conditions may be judged as acceptable (healthy) or unacceptable (unhealthy), depending on prevailing value systems and societal goals. Ecological health indicators characterize the ecological conditions of where we live; improve scientific understanding of ecosystems and the benefits they provide; diagnose causes of ecological degradation; communicate scientific knowledge to nontechnical audiences; guide environmental policy; and measure progress toward societal goals. By helping to identify who benefits and who suffers from societal choices, ecological health indicators inform our choices about how to use or protect ecosystems and their living components, including human society itself.

Book chapter

New indicators of ecological resilience and invasion resistance to support prioritization and management in the sagebrush biome, United States

Ecosystem transformations to altered or novel ecological states are accelerating across the globe. Indicators of ecological resilience to disturbance and resistance to invasion can aid in assessing risks and prioritizing areas for conservation and restoration. The sagebrush biome encompasses parts of 11 western states and is experiencing rapid transformations due to human population growth, invasive species, altered disturbance regimes, and climate change. We built on prior use of static soil moisture and temperature regimes to develop new, ecologically relevant and climate responsive indicators of both resilience and resistance. Our new indicators were based on climate and soil water availability variables derived from process-based ecohydrological models that allow predictions of future conditions. We asked: (1) Which variables best indicate resilience and resistance? (2) What are the relationships among the indicator variables and resilience and resistance categories? (3) How do patterns of resilience and resistance vary across the area? We assembled a large database ( n = 24,045) of vegetation sample plots from regional monitoring programs and derived multiple climate and soil water availability variables for each plot from ecohydrological simulations. We used USDA Natural Resources Conservation Service National Soils Survey Information, Ecological Site Descriptions, and expert knowledge to develop and assign ecological types and resilience and resistance categories to each plot. We used random forest models to derive a set of 19 climate and water availability variables that best predicted resilience and resistance categories. Our models had relatively high multiclass accuracy (80% for resilience; 75% for resistance). Top indicator variables for both resilience and resistance included mean temperature, coldest month temperature, climatic water deficit, and summer and driest month precipitation. Variable relationships and patterns differed among ecoregions but reflected environmental gradients; low resilience and resistance were indicated by warm and dry conditions with high climatic water deficits, and moderately high to high resilience and resistance were characterized by cooler and moister conditions with low climatic water deficits. The new, ecologically-relevant indicators provide information on the vulnerability of resources and likely success of management actions, and can be used to develop new approaches and tools for prioritizing areas for conservation and restoration actions.

Frontiers in Ecology and Evolution

Biological assessments of aquatic ecosystems

The aim of biological assessments (or bioassessments) is to provide decision makers and managers the scientific information and tools needed to protect and restore aquatic life. Biological assessments typically include several critical elements, including development of ecological indicators, indices of ecological status, benchmarks by which to gauge impairment, ways to identify the stressors causing ecological impairment, and biological criteria and standards to protect aquatic life. New scientific tools are emerging that should improve the accuracy and precision of biological assessments, but the major challenges to effective protection and restoration of aquatic life are political and economic rather than scientific.

Book chapter

Adaptive monitoring in action: Reconsidering design-based estimators reveals underestimation of whitebark pine disease prevalence in the Greater Yellowstone Ecosystem

Identifying and understanding status and trends in ecological indicators motivates continual monitoring over decades. Many programs rely on probability surveys and their companion design-based estimators for status assessments (e.g. Horvitz–Thompson). Design-based estimators do not easily extend to trend estimation nor situations with observation errors. Field-based monitoring efforts inevitably have turnover of field crew members which may affect consistency and accuracy of data collection over time. Additionally, design-based estimators ignore the complexities of spatial and temporal heterogeneity in an ecological indicator and how this variability may be linked to environmental or biological dynamics. We propose monitoring programs should re-evaluate their prescribed statistical methods, consider model-based approaches and adapt their sampling designs as needed to improve inferences. The Greater Yellowstone Ecosystem, home to two of the most iconic U.S. National Parks, has experienced significant declines in whitebark pine Pinus albicaulis communities due to forest pathogens, insect outbreaks, wildland fires and drought. Whitebark pine is a keystone species found in mountainous environments throughout the Western U.S. and Canada. We assessed the design-based ratio estimator originally recommended for estimating prevalence of white pine blister rust Cronartium ribicola . We compared the design-based estimator to a model-based approach that accounts for the sampling design, imperfect detection and allows for infection probabilities to vary over space and time. Ignoring observation errors led to lower estimated prevalence of white pine blister rust in the general population. Using model-based approaches, we found that the probability of infection has increased since 2004. However, overall prevalence likely has not changed because of the mountain pine beetle Dendroctonus ponderosae -induced shift towards smaller diameter trees that have a lower probability of infection compared to their larger cohorts. Synthesis and Applications . Using a design-based approach to detect change in ecological indicators falls short because of the inability to account for observation errors or to explore environmental or biological factors explaining temporal dynamics. Inherently understanding the mechanisms leading to changes in an ecological indicator over time informs potential management actions. Our assessment underscores the need for continued evaluation and updating of a monitoring program's sampling design and analytical procedures to maintain relevancy.

Wyoming

The ecological condition of estuaries in the Gulf of Mexico

The Gulf of Mexico is a vast natural resource encompassing the coastal areas of western Florida, Alabama, Mississippi, Louisiana, and Texas, as well as a portion of Mexico. Many estuaries flow into the Gulf of Mexico and serve as nursery grounds for fish, habitat for a wide variety of wildlife, shipping routes, and a source of recreation. Estuarine-dependent species constitute more than 95 percent of the commercial fishery harvests from the Gulf of Mexico, and many important recreational fishery species depend on estuaries during some part of their life cycle. Gulf estuaries are diverse and productive ecosystems that provide a variety of valuable resources, including fish and shellfish, recreation, transportation, and water supply. Assessing the overall condition of Gulf of Mexico estuaries required incorporating data from other federal, state, and local monitoring programs to augment the information on ecological indicators collected by the U.S. Environmental Protection Agency’s (USEPA) Environmental Monitoring and Assessment Program (EMAP). The resulting document would provide a synthesis of the available knowledge about the condition of Gulf of Mexico estuaries. This document is intended for use by scientists and other citizens concerned with the ecological condition of estuaries, as well as by managers and lawmakers interested in the sustained use of estuaries for commercial and recreational purposes. It also addresses public concerns about the aesthetic quality of coastal areas vital to tourism and recreation. By producing this report on the ecological condition of estuaries in the Gulf of Mexico, we have taken one step in assessing the health of this environmental resource. We have produced an environmental “report card” to be used as a guide in the evaluation of management decisions and research directions. This report is organized in three parts: (1) an introduction that gives background information on the Gulf of Mexico, estuarine ecology, and the factors that impact estuaries in the gulf, (2) the main section on priority ecological indicators used to measure the condition of estuaries in the gulf and (3) an ecological report card that summarizes the data on ecological indicators and provides a rating of the condition of estuaries in each gulf state and for gulf estuaries overall. Many of the ratings were based on the percent area of estuaries in each state exhibiting degraded or adverse levels of an indicator. Eutrophication, a condition of high nutrients often resulting in low oxygen levels and other adverse effects, is an important water quality concern for estuaries along the gulf coast. The National Oceanic and Atmospheric Administration (NOAA) has compared the Gulf of Mexico to other coastal regions like the middle Atlantic and has ranked the Gulf of Mexico as having the highest number of point sources of nutrients and the highest percentage of land use devoted to agriculture. We evaluated monitoring data for nitrogen, chlorophyll, and dissolved oxygen as indicators of eutrophication. Although most of the estuaries exhibited high nitrogen or chlorophyll or low dissolved oxygen concentrations at least once during a survey, many times these conditions were observed in small rivers or bayous rather than in the entire estuary. Often, the percent area affected was low. The gulf estuaries had moderate conditions overall for nutrients and dissolved oxygen. Definite nutrient problems were observed in >25% of the estuarine area in Louisiana and Texas and definite dissolved oxygen problems were observed in Alabama. Contaminants in estuarine sediments provide evidence of the accumulation of chemicals from anthropogenic sources. We compared the concentrations of sediment contaminants to established guideline values to determine the proportion of estuarine area that could have potential adverse effects on living organisms. Although detectable levels of contaminants were measured in almost every estuary in the Gulf of Mexico, <25% of the estuarine area in all states had contaminant concentrations that exceeded these guidelines. Wetlands are integral parts of estuarine systems. Declining acreage means habitat loss that may be the result of commercial and residential development, hydrologic alterations, or dredge and fill operations. The Gulf of Mexico region contains more than 50% of the coastal wetland acreage in the U.S. and yet it also has the highest rate of coastal wetland loss. Nine of the top ten estuarine drainage areas ranked by total wetland area are in the Gulf of Mexico region. The most current estimates of total wetland loss over the past 200 years range from 41% to 54% for the gulf states. Although coastal wetlands continue to be altered or destroyed, some estimates indicate that the rate of loss has slowed. All gulf states were rated as having severe problems with wetland loss. The condition of benthic (bottom-dwelling) invertebrates, fish and shellfish, birds, and threatened and endangered species was used to evaluate the health of estuarine fauna. Degraded benthic communities inhabited <25% of the estuarine area in all gulf states except for Texas. Commercial fish and shellfish landings may be used as an indicator of population stability while fish biomarkers are used to measure the health of individuals in the population. Commercial landings of the top four fisheries (shrimp, menhaden, blue crab, and oyster) are stable in the gulf states while fish biomarkers indicate fair to poor fish health in Alabama and Texas and good fish health elsewhere. Coastal and marine bird populations appear to be in good condition throughout the gulf. Four threatened or endangered species inhabit coastal areas in the Gulf of Mexico: brown pelican, Gulf sturgeon, manatee, and Kemp’s ridley sea turtle. In general, populations of these species are in good to fair condition in the gulf states. Public health indicators include shellfish bed closures and chemicals found in edible fish tissue. Harvest of shellfish (primarily oyster in the Gulf of Mexico) is restricted or prohibited when concentrations of bacteria or other pathogens reach levels that could impair human health. The gulf states contain the most acreage of shellfish-growing waters in the U.S. but also have the most acreage restricted for harvest. All gulf states except Mississippi have >25% of their shellfish-growing waters restricted for harvest, mostly due to pollution from wastewater treatment plants or other upstream sources. Advisories may be issued that limit consumption when the concentrations of chemicals in fish tissue exceed levels known to be harmful to humans. Although seafood consumption advisories have been issued in all gulf states, the percent of the fish population with high concentrations of contaminants is relatively low in the gulf overall.

Alabama, Florida, Louisiana, Mississippi, Texas

Are drought indices and climate data good indicators of ecologically relevant soil moisture dynamics in drylands?

Droughts are disproportionately impacting global dryland regions where ecosystem health and function are tightly coupled to moisture availability. Drought severity is commonly estimated using algorithms such as the standardized precipitation-evapotranspiration index (SPEI), which can estimate climatic water balance impacts at various hydrologic scales by varying computational length. However, the performance of these metrics as indicators of soil moisture dynamics at ecologically relevant scales, across soil depths, and in consideration of broader scale ecohydrological processes, requires more attention. In this study, we tested components of climatic water balance, including SPEI and SPEI computation lengths, to recreate multi-decadal and periodic soil-moisture patterns across soil profiles at 866 sites in the western United States. Modeling results show that SPEI calculated over the prior 12-months was the most predictive computation length and could recreate changes in moisture availability within the soil profile over longer periods of time and for annual recharge of deeper soil moisture stores. SPEI was slightly less successful with recreating spring surface-soil moisture availability, which is key to dryland ecosystems dominated by winter precipitation. Meteorological drought indices like SPEI are intended to be convenient and generalized indicators of meteorological water deficit. However, the inconsistent ability of SPEI to recreate ecologically relevant patterns of soil moisture at regional scales suggests that process-based models, and the larger data requirements they involve, remain an important tool for dryland ecohydrology

California, Idaho, Nevada, Oregon, Utah

Use of alligator hole abundance and occupancy rate as indicators for restoration of a human-altered wetland

Use of indicator species as a measure of ecosystem conditions is an established science application in environmental management. Because of its role in shaping wetland systems, the American alligator (Alligator mississippiensis) is one of the ecological indicators for wetland restoration in south Florida, USA. We conducted landscape-level aerial surveys of alligator holes in two different habitats in a wetland where anthropogenic modification of surface hydrology has altered the natural system. Alligator holes were scarcer in an area where modified hydrology caused draining and frequent dry-downs compared to another area that maintains a functional wetland system. Lower abundance of alligator holes indicates lack of alligator activities, lower overall species diversity, and lack of dry-season aquatic refugia for other organisms. The occupancy rate of alligator holes was lower than the current restoration target for the Everglades, and was variable by size class with large size-class alligators predominantly occupying alligator holes. This may indicate unequal size-class distribution, different habitat selection by size classes, or possibly a lack of recruitment. Our study provides pre-restoration baseline information about one indicator species for the Everglades. Success of the restoration can be assessed via effective synthesis of information derived by collective research efforts on the entire suite of selected ecological indicators.

Florida

Identifying indicators of polar bear population status

Monitoring trends in large mammal populations is a fundamental component of wildlife management and conservation. However, direct estimates of population size and vital rates of large mammals can be logistically challenging and expensive. Indicators that reflect trends in abundance, therefore, can be valuable tools for supporting population monitoring. Polar bears have a relatively simple life history such that a few key variables may be effective indicators for tracking changes in body condition and recruitment that affect abundance. Direct estimates of polar bear abundance are difficult to obtain due to their large home ranges in remote Arctic habitats. Changes in abundance associated with environmental conditions appear to affect polar bears largely via effects on female body condition which influence reproduction and cub survival (i.e., recruitment). Loss of sea ice habitat is further limiting researcher access for population monitoring creating a need for alternative approaches. Here we used relationships established from eight years (2008–2017) of data collected on 439 polar bears in the Chukchi Sea, to transform previously published individual-based relationships with annually available sea ice, atmospheric circulation, and prey body condition variables to predict annual mean body condition and recruitment during 2018–2022. Although annual sample sizes were limited for verifying predicted body condition and recruitment via techniques such as cross-validation, in most cases predicted annual means were closely correlated with observed means for 2008–2017. Summer sea ice and prey body condition remained within or increased relative to levels observed during 2008–2017 and predicted polar bear body condition and recruitment during 2018–2022 were largely within or above observed annual means during 2008–2017. A lack of trend in environmental and ecological variables or polar bear body condition and recruitment metrics during 2008–2022 is suggestive that the Chukchi Sea polar bear population was likely stable during this time. Our results provide support for developing models that predict important population parameters of large mammals based on environmental and ecological indicators. Given that trend information is lacking for 10 of the 19 recognized polar bear populations and is outdated for others, the use of environmental and ecological indicators may be particularly useful for augmenting direct estimates of polar bear vital rates in between periods of data collection. Although demographic assessments for polar bears have primarily focused on correlations with sea ice availability, our study and others highlight that prey health is also an important indicator of polar bear population status.

Ecological Indicators

Relationships between indicators of acid-base chemistry and fish assemblages in streams of the Great Smoky Mountains National Park

The acidity of many streams in the Great Smoky Mountains National Park (GRSM) has increased significantly since pre-industrial (∼1850) times due to the effects of highly acidic atmospheric deposition in poorly buffered watersheds. Extensive stream-monitoring programs since 1993 have shown that fish and macroinvertebrate assemblages have been adversely affected in many streams across the GRSM. Matching chemistry and fishery information collected from 389 surveys performed at 52 stream sites over a 22-year period were assessed using logistic regression analysis to help inform the U.S. Environmental Protection Agency’s assessment of the environmental impacts of emissions of oxides of nitrogen (NO x ) and sulfur (SO x ). Numerous logistic equations and associated curves were derived that defined the relations between acid neutralizing capacity (ANC) or pH and different levels of community richness, density, and biomass; and density and biomass of brook trout, rainbow trout, and small prey (minnow) populations in streams of the GRSM. The equations and curves describe the status of fish assemblages in the GRSM under contemporary emission levels and deposition loads of nitrogen (N) and sulfur (S) and provide a means to estimate how newly proposed (and various alternative) target deposition loads, which strongly influence stream ANC, might affect key ecological indicators. Several examples using ANC, community richness, and brook trout density are presented to illustrate the steps needed to predict how future changes in stream chemistry (resulting from different target deposition loads of N and S) will affect the probabilities of observing specific levels of selected biological indicators in GRSM streams. The implications of this study to the regulation of NO x and SO x emissions, water quality, and fisheries management in streams of the GRSM are discussed, but also qualified by the fact that specific examples provided need to be further explored before recommendations concerning their use as ecological indicators could be proposed.

North Carolina, Tennessee

Empirical evaluation of the conceptual model underpinning a regional aquatic long-term monitoring program using causal modelling

Conceptual models are an integral facet of long-term monitoring programs. Proposed linkages between drivers, stressors, and ecological indicators are identified within the conceptual model of most mandated programs. We empirically evaluate a conceptual model developed for a regional aquatic and riparian monitoring program using causal models (i.e., Bayesian path analysis). We assess whether data gathered for regional status and trend estimation can also provide insights on why a stream may deviate from reference conditions. We target the hypothesized causal pathways for how anthropogenic drivers of road density, percent grazing, and percent forest within a catchment affect instream biological condition. We found instream temperature and fine sediments in arid sites and only fine sediments in mesic sites accounted for a significant portion of the maximum possible variation explainable in biological condition among managed sites. However, the biological significance of the direct effects of anthropogenic drivers on instream temperature and fine sediments were minimal or not detected. Consequently, there was weak to no biological support for causal pathways related to anthropogenic drivers&rsquo; impact on biological condition. With weak biological and statistical effect sizes, ignoring environmental contextual variables and covariates that explain natural heterogeneity would have resulted in no evidence of human impacts on biological integrity in some instances. For programs targeting the effects of anthropogenic activities, it is imperative to identify both land use practices and mechanisms that have led to degraded conditions (i.e., moving beyond simple status and trend estimation). Our empirical evaluation of the conceptual model underpinning the long-term monitoring program provided an opportunity for learning and, consequently, we discuss survey design elements that require modification to achieve question driven monitoring, a necessary step in the practice of adaptive monitoring. We suspect our situation is not unique and many programs may suffer from the same inferential disconnect. Commonly, the survey design is optimized for robust estimates of regional status and trend detection and not necessarily to provide statistical inferences on the causal mechanisms outlined in the conceptual model, even though these relationships are typically used to justify and promote the long-term monitoring of a chosen ecological indicator. Our application demonstrates a process for empirical evaluation of conceptual models and exemplifies the need for such interim assessments in order for programs to evolve and persist.

California, Colorado, Idaho, Montana, Nevada, Oreg

An ecological response model for the Cache la Poudre River through Fort Collins

The Poudre River Ecological Response Model (ERM) is a collaborative effort initiated by the City of Fort Collins and a team of nine river scientists to provide the City with a tool to improve its understanding of the past, present, and likely future conditions of the Cache la Poudre River ecosystem. The overall ecosystem condition is described through the measurement of key ecological indicators such as shape and character of the stream channel and banks, streamside plant communities and floodplain wetlands, aquatic vegetation and insects, and fishes, both coolwater trout and warmwater native species. The 13- mile-long study area of the Poudre River flows through Fort Collins, Colorado, and is located in an ecological transition zone between the upstream, cold-water, steep-gradient system in the Front Range of the Southern Rocky Mountains and the downstream, warm-water, low-gradient reach in the Colorado high plains. The City wanted to better understand the ecological response of the Poudre River ecosystem to potential changes in stream flow and other physical parameters through the conceptual framework of a multivariable integrated model. This goal was met through the use of a probabilistic model based on Bayesian concepts. This construct allowed the integration of a wide range of data and expert opinion (as informed by local data) to predict potential changes to ecosystem conditions under various flow scenarios. Nine flow scenarios representing past, present, and possible future hydrology were developed as the primary model input. Both reach-scale drivers such as stream channel conditions and pollutant loads, as well as ecological conditions, including species composition, interactions, and habitat requirements influenced model-predicted ecosystem outcomes. Model output consisted of probability distributions for eight ecological indicators collectively representing the physical setting, aquatic life, and riparian habitats of the river ecosystem. We are confident in model predictions related to probable trends, relative magnitude of changes and potential ecosystem responses to changing flow conditions, though data availability and the process of converting diverse data types into a common unit (probabilities) limit precision of individual results. Key findings suggest that: The present ecological function of the Poudre River is altered as a result of more than 150 years of human influences that include highly managed flows, urbanization, gravel mining, channelization and urban and industrial encroachment in the floodplain, underscoring the vulnerable and complex character of the Poudre River; A continuation of today&rsquo;s flow management will lead to ongoing changes in ecosystem condition, and additional water depletions will compromise ecological conditions; High flows play an essential role in maintaining and improving the aquatic and riparian condition of the river; Adequate flows in base-flow periods are critical to desirable water quality, and thriving fish and insect populations; Improvement of native aquatic life is possible if issues related to channel modifications, siltation, invasive species, and base and high flow conditions are managed properly; The present confined river channel and modified flows has reduced the potential for a keystone and iconic species, plains cottonwood, to be self-sustaining in the study area; The streamside corridor retains the potential to support a functioning riparian forest that provides important ecological services if periodic floodplain inundation occurs. Environmental flows that combine stable and adequate flows in base-flow periods with occasional rejuvenating high flows that meet target levels defined in this study are likely improve all biological indicators across the system. ERM test scenarios that include both stable base flows and rejuvenating high flows indicate that substantial improvements in the river ecosystem can be achieved with improved management of flow volumes similar to those observed in the river during the last half century of intensive water development. These results underscore the possibility of improving the river ecosystem through active management while still maintaining the Poudre&rsquo;s diverse economic benefits and role as a working river. The ERM was designed to represent the multi-dimensional ecological character of the contemporary urban Poudre River. It provides a scientific foundation that can serve as a decision support tool and foster a more informed community discussion about the future of the river as it provides a better understanding of the likely response of the Poudre River ecosystem to environmental flow management and other stewardship activities. In particular, model results can assist managers in developing specific management actions to achieve desirable goals for key indicators of river health.

Colorado

Heed the data gap: Guidelines for using incomplete datasets in annual stream temperature analyses

Stream temperature data are useful for deciphering watershed processes important for aquatic ecosystems. Accurately extracting signal trends from stream temperature is essential for predicting responses of environmental and ecological indicators to change. Missing data periods are common for various reasons, and pose a challenge for scientists using temperature signal analysis to support stream research and ecological management objectives. However, the sensitivity of estimated temperature signal patterns to missing data has not been thoroughly evaluated, despite the potentially large impact on interpretation. In this study, we explored the effects of simulated missing daily data on the characterization of annual water temperature signals measured at headwater sites in the Pacific Northwest and Mid-Atlantic regions of the USA. For each site, we used linear regressions of sine-waves fitted to complete (365-d) and partial (7–357 consecutive missing data points) annual datasets of daily mean water temperature and computed three thermal parameters (mean, phase, and amplitude), which together can indicate thermally and ecologically influential watershed processes (e.g., depth and magnitude of groundwater discharge). Expected values (derived from complete datasets) ranged from 7.0 to 12.6 °C, 205 to 254 d, and 1.9 to 9.5 °C for annual mean, phase, and amplitude, respectively. While annual phase and amplitude could be accurately estimated (i.e., within 95–99% confidence intervals of expected values) with up to approximately two months of consecutively missing data, annual mean temperature required more complete datasets. We found that datasets with less than seven weeks of consecutively missing data enabled estimation of all annual signal parameters with reasonable accuracy (>75% probability of being within the 95–99% confidence intervals of expected values). Imputation of missing data expanded this range to approximately 20 weeks, with the greatest improvements in parameter estimation between 9 and 27 weeks of imputed missing data. However, caution should be exercised when applying this technique. For example, imputation improved the accuracy of parameter estimation for most sites, but accuracy decreased for some sites exhibiting strong groundwater influence. The timing of consecutive missing data points within a year had inconsistent effects on annual thermal parameter estimates among regions, years, and individual parameters. Utilizing sites with more than approximately seven consecutive weeks of missing data or 20 weeks of imputed data increases the probability of mischaracterization of annual stream thermal regimes. Understanding this limitation is vital for identifying the potential of streams to serve as climate refugia for ecological indicator species and effective future management of stream systems.

Ecological Indicators