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Niobium-rich minerals from the Sheep Creek carbonatite-related deposits, Montana, USA

The Sheep Creek area in southwest Montana contains a number of small, tabular, carbonatite-related deposits that are rich in Nb- and REE-minerals. Two of the “vein-dikes” were mined at a small scale for Nb in the late 1950s. The most abundant Nb-bearing minerals are columbite-(Fe), nioboaeschynite-(Ce), baotite, and two varieties of Ti-Nb-Fe-oxide. An early oxide with Ti/Nb (atomic) near unity later broke down to a mixture of columbite + TiO2 with distinctly lower Nb content. Zircon intergrown with the Nb-REE minerals was dated by U-Pb methods to 116±2 Ma. This age is in agreement with previous dating (Th-Pb on monazite, Re-Os on molybdenite), and indicates that the Sheep Creek deposits are early Cretaceous in age.

Montana

Rare earth elements on the Moon

Rare earth elements (REEs) are a scarce but vital resource for our modern economies and lifestyles. Since the late 1990s, China has supplied the vast majority of the world’s refined REEs. Increasing global demand has broadened the search for REE deposits to unconventional places, including the Moon. Although most lunar rocks have very low REE concentrations, Apollo samples showed that one type of lunar rock containing potassium (K), REEs, and phosphorus (P)—known by the acronym KREEP—has high concentrations of REEs. Data from orbiting satellites have identified locations where substantial deposits of KREEP are likely. The viability of mining these deposits depends on the evolution of REE economics, the development of the Earth-Moon infrastructure, and the findings from future lunar mineral exploration missions.

Fact Sheet

Evaluation of an open earthquake early warning system in Mexico, and laboratory tests of their sensors

Microelectromechanical system (MEMS) accelerometers are useful for seismological and engineering applications because of their ability to record unsaturated large seismic signals. Recent advances in MEMS technologies enable the design of instruments with improved capabilities that also allow the recording of small signals. As a result, MEMS can be useful across a broad dynamic range and for both major earthquakes and smaller magnitude events. Here, we analyze the performance of a MEMS‐based accelerometer with the capability of real‐time data transmission. We discuss the self‐noise level, dynamic range, and sensitivity, along with the timing precision and data transmission latency, and compare these parameters to other MEMS‐based instruments. We also summarize observations from a regional network deployed in southern Mexico over a period of 3+ yr for the purpose of earthquake early warning. In addition to the sensor evaluation, we present a large, openly available data set of strong motion data from the Mexican network that comprises continuous ground‐motion records from 24 accelerometers in the period between 2017 and 2022.

Seismological Research Letters

Special issue "Effects of surface geology on seismic motion (ESG): General state-of-research"

To understand and predict the behaviors of strong ground motions incurred during devastating earthquakes, studies focused on the “effects of surface geology (ESG) on seismic motions” (following tradition, herein contracted as: “ESG”) have progressed steadily in the last three decades. These ESG studies typically involve in situ measurements of the wavefield and commonly apply both analytical and computational approaches. Concurrently, improvements in ESG related research can be readily attributed to the proliferation of openly accessible strong motion data, as well as advances in computational power. Nevertheless, there remain significant shortfalls in our understanding of the epistemic and aleatory uncertainties associated with the ESG as demonstrated by phenomena from recent deadly earthquake-related site effects. Thus, investigations toward addressing these deficiencies should be underscored in future earthquake related disaster mitigation efforts. This special issue of Earth, Planets, and Space (EPS) is dedicated to the ongoing efforts by the International Association of Seismology and Physics of the Earth’s Interior (IASPEI) and the International Association of Earthquake Engineering (IAEE) that champion activities promoting studies related to the ESG.

Earth, Planets and Space

Precipitation-based flood-inundation maps for the East Fork Little Blue River and tributaries at Lee’s Summit, Missouri, 2024

The U.S. Geological Survey, in cooperation with the City of Lee’s Summit, Missouri, assessed flooding of the East Fork Little Blue River and tributaries for varying precipitation magnitudes and durations, varying antecedent runoff conditions, and projected climate-change conditions. The precipitation scenarios were used to develop a library of flood-inundation maps for a 2.95-mile reach of the East Fork Little Blue River and tributaries within the city. A two-dimensional U.S. Army Corps of Engineers Hydrologic Engineering Center–River Analysis System (HEC–RAS; ver. 6.5) rain-on-grid model was calibrated to selected runoff events representing a range of antecedent runoff conditions and hydrologic responses. Lowest adjacent grades for structures within the nearby study area were incorporated into the terrain, and depth grids and water-surface elevation grids were developed for the study area. Simulated velocities at selected bridge locations were also developed from the model. The model was calibrated using water-surface elevation data collected from water-level loggers (pressure transducers) and streamflow measurements and water-surface elevation measurements made at a reference point during runoff events. The calibrated HEC–RAS model was used to simulate streamflows from design rainfall events of 15-minute to 24-hour durations and ranging from a 100- to 0.1-percent annual exceedance probability (1-year to 1,000-year recurrence intervals). Flood-inundation maps were produced for depths at a reference location of 3 to 16 feet, or a depth exceeding the 0.1-percent annual exceedance probability interval precipitation. The results of each precipitation duration-frequency value were represented by a 1-foot-increment inundation map based on the generated peak streamflow from that rainfall event and the corresponding water-surface elevation at the East Fork Little Blue River reference location. Within the HEC–RAS model, 240 scenarios were developed from the design rainfall events with each of 3 antecedent conditions. Additional scenarios were created to simulate the effects of projected precipitation scenarios on the 100-year recurrence interval, 24-hour storm and the 100-year recurrence interval, 6-hour storm. All simulation results were assigned to a flood-inundation map condition based on the generated peak flow and corresponding water-surface elevation at the East Fork Little Blue River reference location. The flood-inundation maps are shown on a web mapping application made available to the public through the City of Lee’s Summit (hyperlink will be added when available). The flood-inundation maps are tied to real-time precipitation data obtained from the Automated Surface Observing System weather station at the Lee’s Summit Municipal Airport, accessible at https://mesonet.agron.iastate.edu/request/download.phtml?network=MO_ASOS . The availability of these maps, along with information regarding observed rainfall, could help provide emergency management personnel and residents with information that is critical for flood-response activities, such as evacuations and road closures, and for postflood recovery efforts.

Missouri

Assessment of the hydraulic connection between ground water and the Peace River, west-central Florida

The hydraulic connection between the Peace River and the underlying aquifers along the length of the Peace River from Bartow to Arcadia was assessed to evaluate flow exchanges between these hydrologic systems. Methods included an evaluation of hydrologic and geologic records and seismic-reflection profiles, seepage investigations, and thermal infrared imagery interpretation. Along the upper Peace River, a progressive long-term decline in streamflow has occurred since 1931 due to a lowering of the potentiometric surface of the Upper Floridan aquifer by as much as 60 feet because of intensive ground-water withdrawals for phosphate mining and agriculture. Another effect from lowering the potentiometric surface has been the cessation of flow at several springs located near and within the Peace River channel, including Kissengen Spring, that once averaged a flow of about 19 million gallons a day. The lowering of ground-water head resulted in flow reversals at locations where streamflow enters sinkholes along the streambed and floodplain. Hydrogeologic conditions along the Peace River vary from Bartow to Arcadia. Three distinctive hydrogeologic areas along the Peace River were delineated: (1) the upper Peace River near Bartow, where ground-water recharge occurs; (2) the middle Peace River near Bowling Green, where reversals of hydraulic gradients occur; and (3) the lower Peace River near Arcadia, where ground-water discharge occurs. Seismic-reflection data were used to identify geologic features that could serve as potential conduits for surface-water and ground-water exchange. Depending on the hydrologic regime, this exchange could be recharge of surface water into the aquifer system or discharge of ground water into the stream channel. Geologic features that would provide pathways for water movement were identified in the seismic record; they varied from buried irregular surfaces to large-scale subsidence flexures and vertical fractures or enlarged solution conduits. Generally, the upper Peace River is characterized by a shallow, buried irregular top of rock, numerous observed sinkholes, and subsidence depressions. The downward head gradient provides potential for the Peace River to lose water to the ground-water system. Along the middle Peace River area, head gradients alternate between downward and upward, creating both recharging and discharging ground-water conditions. Seismic records show that buried, laterally continuous reflectors in the lower Peace River pinch out in the middle Peace River streambed. Small springs have been observed along the streambed where these units pinch out. This area corresponds to the region where highest ground-water seepage volumes were measured during this study. Further south, along the lower Peace River, upward head gradients provide conditions for ground-water discharge into the Peace River. Generally, confinement between the surficial aquifer and the confined ground-water systems in this area is better than to the north. However, localized avenues for surface-water and ground-water interactions may exist along discontinuities observed in seismic reflectors associated with large-scale flexures or subsidence features. Ground-water seepage gains or losses along the Peace River were quantified by making three seepage runs during periods of: (1) low base flow, (2) high base flow, and (3) high flow. Low and high base-flow seepage runs were performed along a 74-mile length of the Peace River, between Bartow and Nocatee. Maximum losses of 17.3 cubic feet per second (11.2 million gallons per day) were measured along a 3.2-mile reach of the upper Peace River. The high-flow seepage run was conducted to quantify losses in the Peace River channel and floodplain between Bartow and Fort Meade. Seepage losses calculated during high-flow along a 7.2-mile reach of the Peace River, from the Clear Springs Mine bridge to the Mobil Mine bridge, were approximately 10 percent of the river flow, or 118 cubic feet per second. Calculated seepages along the Peace River in Hardee and De Soto Counties were inconclusive, because most seepages were within the range of discharge measurement error. Two continuous aerial thermal infrared imagery surveys were conducted to locate sites of ground-water discharge along the Peace River. Although temperature and hydrologic conditions were ideal to observe spring flow using thermal infrared imaging techniques, no sources of ground-water discharge were identified using this method. Diffuse ground-water seepage may, however, provide significant ground-water discharge.

Florida

Comparison of two approaches for determining ground-water discharge and pumpage in the lower Arkansas River Basin, Colorado, 1997-98

In March 1994, the Colorado Division of Water Resources (CDWR) adopted “Rules Governing the Measurement of Tributary Ground Water Diversions Located in the Arkansas River Basin” (Office of the State Engineer, 1994); these initial rules were amended in February 1996 (Office of the State Engineer, 1996). The amended rules require users of wells that divert tributary ground water to annually report the water pumped monthly by each well. The rules allow a well owner to report the pumpage measured by a totalizing flowmeter (TFM) or pumpage determined from electrical power data and a power conversion coefficient (PCC) (Hurr and Litke, 1989). Opinions by representatives of the State of Kansas, presented before the Special Master hearing a court case [State of Kansas v. State of Colorado, No. 105 Original (1996)] concerning post-Compact well pumping, stated that the PCC approach does not provide the same level of accuracy and reliability as a TFM when used to determine pumpage. In 1997, the U.S. Geological Survey (USGS), in cooperation with the CDWR, began a 2-year study to compare ground-water pumpage estimates made using the TFM and the PCC approaches. The study area was along the Arkansas River between Pueblo, Colorado, and the Colorado-Kansas State line (fig. 1). The two approaches for estimating ground-water discharge and pumpage were compared for more than 100 wells completed in the alluvial aquifer of the Arkansas River Basin. The TFM approach uses an inline flowmeter to directly measure instantaneous discharge and the total volume of water pumped at a well. The PCC approach uses electrical power consumption records and a power conversion coefficient to estimate the pumpage at ground-water wells. This executive summary describes the results of the comparison of the two approaches. Specifically, (1) the differences in instantaneous discharge measured with three portable flowmeters and measured with an inline TFM are evaluated, and the statistical differences in paired instantaneous discharge between the two approaches are determined; (2) short- and long-term variations in the PCC’s are presented; (3) differences in pumpage between the two approaches are evaluated, and the statistical differences in pumpage between the two approaches are determined; (4) potential sources of discrepancy between pumpage estimates are discussed; and (5) differences in total network pumpage using the two approaches are presented. During the irrigation seasons of 1997 and 1998, instantaneous discharge and electrical power demand were measured at randomly selected wells to determine PCC’s. At more than 100 wells, the PCC’s determined during the 1998 season were applied to total electrical power consumption data that was recorded between the initial and final readings at each network well site in 1998 to estimate total ground-water pumpage. At each site, an inline TFM was installed in a full-flowing, acceptable test section of pipe on the discharge side of the pump where the measurement of discharge was made. Measurements of instantaneous ground-water discharge also were made using three different types of portable flowmeters. The average velocity multiplied by the cross-sectional area of the discharge pipe was used to compute the discharge in gallons per minute. Whenever possible, discharge measurements were made at each network site using all three types of portable flowmeters.

Colorado

Critical minerals in orogenic (gold) and Coeur d’Alene-type mineral systems of the United States

Orogenic and Coeur d’Alene-type mineral systems are produced by metamorphic devolatilization of thick volcanic or siliciclastic sedimentary rock sequences and the focused flow of hydrothermal fluids upwards along crustal-scale faults. Most orogenic systems are found along the Cordilleran orogen, stretching from California northwards into Alaska, whereas most Coeur d’Alene-type systems occur in the Proterozoic Belt Basin in Idaho and Montana. Although the deposit types in these systems are exploited for precious and base metals, potential exists for the production of critical minerals necessary for current (2025) societal needs in the United States. Publicly available geochemical data compiled for these mineral systems, coupled with mineralogical characteristics, indicate that several critical minerals could potentially be recovered from unmined resources and processed mine waste: arsenic, antimony, tellurium, cobalt, and tungsten from orogenic gold deposits and zinc, antimony, arsenic, and manganese from Coeur d’Alene-type systems. These critical minerals reside primarily in arsenopyrite (arsenic), scheelite (tungsten), siderite (manganese), sphalerite (zinc), tetrahedrite (antimony and arsenic), stibnite (antimony), and telluride (tellurium) minerals.

continental United States

Critical mineral inventory of select IOA-IOCG deposits, southwestern USA

Critical minerals are necessary for modern technology and strategic purposes. Their increasing importance requires finding new and nontraditional resources. Samples of ore, altered, and unaltered host rock were collected from 26 iron mines and prospects in California, Nevada, and Utah to assess the potential of these deposits to host economic quantities of different critical minerals. Geochemical analyses were conducted by 61 element ICP-OES-MS sodium peroxide fusion and major elements determined by WDXRF. These deposits concentrated many critical minerals beyond what is found in average upper crustal abundances, such as Sb, As, Bi, Co, Ga, Mg, Mn, Ni, Nb, Pd, REE, Sc, Te, Sn, Ti, W, V, and Zn. However, most of these are not concentrated enough in the ore to be considered as economic resources. Those critical minerals that are enriched enough in some of these deposits to possibly be considered as by-product commodities are Ni, REE, V, and potentially Co and Ga. These enrichments were not uniform, with REE more likely to be enriched in IOA deposits, whereas Co, Ga, Ni, and V could be found enriched in either IOA or IOCG deposits.

California, Nevada, Utah

A 700-year rupture sequence of great eastern Aleutian earthquakes from tsunami modeling of stratigraphic records

Great Aleutian underthrusting earthquakes produced destructive tsunamis impacting Hawaiʻi in 1946 and 1957. Prior modeling of the 1957 tsunami deposit and runup records on eastern Aleutian and Hawaiian Islands jointly with tide-gauge observations across the Pacific Ocean constrained a rupture model with shallow slip up to 26 m along 600 km of the plate boundary. Here we implement this modeling approach to older deposits and show alternating deep and shallow megathrust slip up to 26, 32, and 22 m for great earthquakes along the same segment in the 18 th , 15 th , and 14 th centuries. All three modeled prehistoric Aleutian earthquakes produce tsunami inundation in Hawaiʻi with the most severe, 14 th century event having impacts exceeding the 1957 event. The along-dip variability of these four ruptures spanning seven centuries provides insights on earthquake cycles for engineering design and hazard assessment. The 15 th century and 1957 rupture models provide evidence for recurrence of tsunami earthquakes, which can produce disproportionately large tsunamis for a given moment magnitude due to reduced rigidity in the shallow megathrust. The 14 th and 18 th century events likely ruptured deeper regions that did not slip in 1957, suggesting potential for corresponding deeper failure in the next great eastern Aleutian earthquake.

Alaska, Hawaii

On the provenance of field reports of the 1886 Charleston, South Carolina, earthquake: A seismo-historical whodunnit

Much of what is known about the effects of the 1886 Charleston, South Carolina, earthquake throughout the epicentral region can be attributed to meticulous field investigations by an individual with training in geology and engineering, Earle Sloan ( Clendenin, 1926 ). In a recent study, Bilham and Hough (2024) undertook a detailed analysis of the effects of the earthquake on railroads in the Charleston region, drawing heavily from Sloan’s reports. This exercise identified several inconsistencies in Sloan’s field reports, including understandable measurement imprecision, inferred data entry mistakes, and transcription errors. The study also begged the question, where was Sloan at the time of the mainshock and over the following week? And to what extent did he draw from secondhand information in compiling his reports? On this question Sloan’s reports were sometimes enigmatic, lending themselves to misinterpretation in contemporaneous as well as modern interpretations. Beyond the details that were germane for, and briefly summarized by, the studies of Bilham and Hough (2023 , 2024) , in this report we don our historical seismologist caps to chronicle Sloan’s activities following the earthquake. We summarize our inferences here for the benefit of future scholars who might attempt to retrace either Sloan’s footsteps or our own. This study also serves to highlight Sloan’s singular contributions to earthquake science, which were never published separately.

South Carolina

Orientation dependence of probabilistic seismic hazard estimates from CyberShake physics-based simulations

Earthquake ground‐motion intensities, such as pseudospectral accelerations (SAs), can vary significantly with horizontal orientation. However, conducting probabilistic seismic hazard analysis (PSHA) for each horizontal orientation is challenging because current ground‐motion models used in PSHA consider only a single horizontal intensity value, usually the median across all orientations, known as RotD50. To address this limitation, we employ physics‐based simulations for PSHA, which contain full waveforms from which ground‐motion intensities can be computed for all horizontal orientations to study directional seismic hazard. We apply our approach to the latest CyberShake study of the Greater Los Angeles metropolitan area, developed by the Statewide California Earthquake Center, finding that seismic hazard at a 2475‐yr return period, a common value used for earthquake‐resistant design, varies significantly with horizontal orientation. For instance, for SAs at 3 s, the maximum seismic hazard across all horizontal orientations is, on average, 15% higher than the median RotD50 hazard, with these differences becoming more pronounced at longer periods. These observed variations can generally be attributed to physical mechanisms that polarize seismic waves, such as the radiation pattern of the earthquake source and the influence of the subsurface structure. These results may have important implications for earthquake engineering applications, particularly for long‐period structures in areas with substantial horizontal variations in seismic hazard.

California

Stable isotope composition and geochemistry of calcite and dolomite in the Mountain Pass carbonatite: A lens into petrogenesis

Carbonatites host most of the global rare earth element (REE) deposits. The petrogenesis of these rocks, including magmatic and post-magmatic processes, are poorly understood but critical in forming and upgrading these deposits. The Mountain Pass carbonatite, which hosts the only active REE mine in North America, is lithologically variable but consistently contains >50% calcite and/or dolomite. Multiple generations of carbonates are evident, suggesting bulk analysis of carbonate minerals would generate a mixed isotopic and geochemical signature. In-situ stable carbon and oxygen isotopes, combined with geochemical analysis and petrography, distinguish carbonate generations, trace the source region of the carbonatite magma, and link carbonate genesis to REE mineralization throughout the intrusion. Primary carbon and oxygen isotope values outside the accepted mantle range may suggest a subduction-related carbon and oxygen source in the mantle. Deformation textures in primary calcite indicate syn- or post-crystallization ductile flow. Carbonates with secondary textures and heavy δ 13 C and δ 18 O values are associated with ore mineralization, suggesting fluid mobilization was important in concentrating REE and upgrading of the Mountain Pass deposit.

California

Sedimentary exhalative (sedex) zinc-lead-silver deposit model

This report draws on previous syntheses and basic research studies of sedimentary exhalative (sedex) deposits to arrive at the defining criteria, both descriptive and genetic, for sedex-type deposits. Studies of the tectonic, sedimentary, and fluid evolution of modern and ancient sedimentary basins have also been used to select defining criteria. The focus here is on the geologic characteristics of sedex deposit-hosting basins that contain greater than 10 million metric tons of zinc and lead. The enormous size of sedex deposits strongly suggests that basin-scale geologic processes are involved in their formation. It follows that mass balance constraints of basinal processes can provide a conceptual underpinning for the evaluation of potential ore-forming mechanisms and the identification of geologic indicators for ore potential in specific sedimentary basins. Empirical data and a genetic understanding of the physicochemical, geologic, and mass balance conditions required for each of these elements are used to establish a hierarchy of quantifiable geologic criteria that can be used in U.S. Geological Survey national assessments. In addition, this report also provides a comprehensive evaluation of environmental considerations associated with the mining of sedex deposits.

Scientific Investigations Report

Forecasting storm-induced coastal flooding for 21st century sea-level rise scenarios in the Hawaiian, Mariana, and American Samoan Islands

Oceanographic, coastal engineering, ecologic, and geospatial data and tools were combined to evaluate the increased risks of storm-induced coastal flooding in the populated Hawaiian, Mariana, and American Samoan Islands as a result of climate change and sea-level rise. We followed a hybrid (dynamical and statistical) downscaling approach to map flooding due to waves and storm surge at 10-square meter resolution along all 1,870 kilometers of these islands’ coastlines for annual (1-year), 20-year, and 100-year return-interval storm events and +0.00 meter (m), +0.25 m, +0.50 m, +1.00 m, +1.50 m, +2.00 m, and +3.00 m sea-level rise scenarios. We quantified the coastal flood depths and extents using the latest climate forcing from Intergovernmental Panel for Climate Change’s Sixth Assessment Report Coupled Model Intercomparison Project. The data generated using these methods provide stakeholders and decision makers with a spatially explicit, rigorous valuation of how, where, and when climate change and sea-level rise increase coastal storm-induced flooding to help identify areas where management and (or) restoration could potentially help reduce the risk to, and increase the resiliency of, the coastal communities in the populated Hawaiian, Mariana, and American Samoan Islands.

Hawaii

Rewilding the upper Klamath River Basin: Rapid recolonization of Chinook salmon following the world’s largest dam removal

Habitat fragmentation is a global driver of decline in biodiversity, yet evaluations of ecological response following re-connection remain rare. In 2024, four hydroelectric dams on the Klamath River were removed, reconnecting over 640 km of anadromous habitat and marking the largest dam removal project completed to date. We used a stationary high-resolution multi-beam imaging SONAR to quantify adult Chinook salmon ( Oncorhynchus tshawytscha ) passage into newly reopened reaches during the first two migratory seasons following dam removal. An estimated 7,742 (95% CI: 7,702–7,778) Chinook salmon migrated upstream in 2024 and 13,310 (95% CI: 12,876–13,733) in 2025, representing 18–19% of fish returning to the Klamath River Basin. Within this period, Chinook salmon recolonized 88% of their documented historical distribution, expanding into 345 km of reconnected habitats and reaching elevations of up to 1,250 m. Recolonization required fish to swim through dam removal sites, complex hydraulic corridors and engineered fishways, demonstrating the propensity for rapid recolonization at basin scale despite these challenges. These findings provide the first quantitative assessment of salmon response to the world’s largest dam removal and demonstrate how reconnection can catalyze large-scale recolonization in historically fragmented river networks.

California, Oregon

Detecting hidden sedimentary geothermal systems in the Upper Colorado River Basin

Geothermal resources exist in sedimentary rock where circulation of water for efficient extraction or storage of heat is possible. Except in rare instances where hot water is expressed at the land surface, sedimentary geothermal resources are hidden, so the identification of these systems is optimally accomplished using predictive subsurface modeling. An integrated approach using detailed paleogeographic interpretations, subsurface geologic mapping, and numerical modeling has produced regional geologic and temperature models for the Upper Colorado River Basin, a large watershed in central North America that contains many sedimentary basins. These models identify areas of hidden sedimentary geothermal resource potential in low temperature (<90°C), moderate temperature (90–150°C), and high temperature (>150°C) fairways across the study area. These models incorporate maps of key horizons in outcrop and the subsurface to create a robust structural framework that can be used to target favorable geology for natural or engineered permeability. This framework is populated with lithologies derived from detailed palaeogeographical maps and over 40,000 bottom hole temperature (BHT) values were used to create a calibrated three-dimensional (3D) temperature model across the region. The resulting maps serve as a regional sedimentary geothermal play fairway screening tool for evaluating different grades of sedimentary geothermal resources and for identifying areas of interest where more detailed, prospect-scale studies can be undertaken.

Arizona, Colorado, New Mexico, Utah, Wyoming

Triple-oxygen isotopic evidence of prolonged direct bioleaching of pyrite with O2

Sulfate is often touted as containing atmospheric oxygen whose isotopic signature can constrain redox, environmental conditions, and biological activity. Yet, the amount and isotopic fractionation associated with air-O 2 incorporation during sulfate formation is still debated, making its verification difficult. In this study, we identify a distinct, microbially dominated environment with the potential to preserve maximum signals of air-O 2 in sulfate. We report triple-oxygen isotope data for sulfate produced from pyrite oxidation in microbial and abiotic experiments, and from natural dissolved sulfate from the Rio Tinto, Spain, an acid mine drainage site. The oxygen isotope systematics of sulfate in these environments define a unique kinetic isotope effect associated with initial stage pyrite oxidation by Acidithiobacillus ferrooxidans that preserves >80 % oxygen from air-O 2 in sulfate. Unlike experiments, which evolve toward water-oxygen dominated sulfate on short time scales, Rio Tinto, Spain hosts a microbe rich environment with distinct geochemistry that maintains high O 2 -oxygen in sulfate. Therefore, in addition to containing isotopic records from water and air, sulfates can also contain a biosignature that is promising for understanding conditions on Mars and early Earth.

Earth and Planetary Science Letters