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Concentrations, and estimated loads and yields of nutrients and suspended sediment in the Little River basin, Kentucky, 2003-04

Nutrients, primarily nitrogen and phosphorus compounds, naturally occur but also are applied to land in the form of commercial fertilizers and livestock waste to enhance plant growth. Concentrations, estimated loads and yields, and sources of nitrite plus nitrate, total phosphorus, and orthophosphate were evaluated in streams of the Little River Basin to assist the Commonwealth of Kentucky in developing 'total maximum daily loads' (TMDLs) for streams in the basin. The Little River Basin encompasses about 600 square miles in Christian and Trigg Counties, and a portion of Caldwell County in western Kentucky. Water samples were collected in streams in the Little River Basin during 2003-04 as part of a study conducted in cooperation with the Kentucky Department of Agriculture. A total of 92 water samples were collected at four fixed-network sites from March through November 2003 and from February through November 2004. An additional 20 samples were collected at five synoptic-network sites during the same period. Median concentrations of nitrogen, phosphorus, and suspended sediment varied spatially and seasonally. Concentrations of nitrogen were higher in the spring (March-May) after fertilizer application and runoff. The highest concentration of nitrite plus nitrate-5.7 milligrams per liter (mg/L)-was detected at the South Fork Little River site. The Sinking Fork near Cadiz site had the highest median concentration of nitrite plus nitrate (4.6 mg/L). The North Fork Little River site and the Little River near Cadiz site had higher concentrations of orthophosphate in the fall and lower concentrations in the spring. Concentrations of orthophosphate remained high during the summer (June-August) at the North Fork Little River site possibly because of the contribution of wastewater effluent to streamflow. Fifty-eight percent of the concentrations of total phosphorus at the nine sites exceeded the U.S. Environmental Protection Agency recommended maximum concentration limit of 0.1 mg/L. Concentrations of suspended sediment were highest in the spring during runoff and lowest in the fall. The highest concentration of suspended sediment (1,020 mg/L) was observed at the Sinking Fork near Cadiz site. The median concentration of suspended sediment for all sites sampled was 12 mg/L. A nonparameteric statistical test (Wilcoxson rank-sum) showed that the median concentrations of suspended sediment were not different among any of the fixed-network sites. The Little River near Cadiz site contributed larger estimated mean annual loads of nitrite plus nitrate (2,500,000 pounds per year (lb/yr)) and total phosphorus (160,000 lb/yr) than the other three fixed-network sites. Of the two main upstream tributaries from the Little River near Cadiz site, the North Fork Little River was the greatest contributor of total phosphorus to the study area with an estimated mean annual load of 107,000 lb/yr or about 64 percent of the total estimated mean annual load at the Little River near Cadiz site. The other main upstream tributary, South Fork Little River, had an estimated mean annual load of total phosphorus that was about 20 percent of the mean annual load at the Little River near Cadiz site. Estimated loads of suspended sediment were largest at the Little River near Cadiz site, where the estimated mean annual load for 2003-04 was about 84,000,000 lb/yr. The North Fork Little River contributed an estimated 36 percent of the mean annual load of suspended sediment at the Little River near Cadiz site, while the South Fork Little River contributed an estimated 18 percent of the mean annual load at the Little River near Cadiz site. The North Fork Little River site had the largest estimated mean annual yield of total phosphorus (1,600 pounds per year per square mile (lb/yr/mi2)) and orthophosphate (1,100 lb/yr/mi2). A principal source of phosphorus for the North Fork Little River is discharge from wastewater-treatment facilities. The largest estimated mean annual yield of nitrite plus nitrate was observed at the South Fork Little River site. The North Fork Little River site had the largest estimated mean annual yield of suspended sediment (450,000 lb/yr/mi2). Inputs of nitrogen and phosphorus to streams from point and nonpoint sources were estimated for the Little River Basin. Commercial fertilizer and livestock-waste applications on row crops are a principal source of nutrients for most of the Little River Basin. Sources of nutrients in the urban areas of the basin mainly are from effluent discharge from wastewater-treatment facilities and fertilizer applications to lawns and golf courses.

Scientific Investigations Report↗

Occurrence of organic wastewater-indicator compounds in urban streams of the Atlanta area, Georgia, 2003-2006

Between March 2003 and January 2006, 863 water samples were collected from streams in seven urban watersheds with varying land uses within or near the City of Atlanta, Georgia. Sixty-four sampling sites representing three site types were established in those watersheds. The first type consisted of sites within three watersheds not affected by combined sewer overflows; these were designated as the control basins. The second and third site types were established in four watersheds and were designated as sites upstream or downstream from combined sewer outfalls. Stream samples collected during the study were analyzed for major ions, nutrients, trace metals, and 60 organic compounds commonly found in wastewater (organic wastewater-indicator compounds, OWICs). Inorganic constituents were analyzed to discern possible relations between OWICs and urban runoff, sewage effluent, or combined sewer overflows (CSOs). The OWICs were grouped into nine compound classes based either on an already accepted class of compounds (such as pesticide) or the manner in which the compounds are used (such as automotive uses). The compounds benzo(a)pyrene, 4-cumylphenol, 3-tert-butyl-4-hydroxyanisole (BHA), isophorone, isoquinoline, metolachlor, metalaxyl, and 4-octylphenol were not detected in any sample collected during the study. As many as 33 OWICs were detected above study reporting levels in water samples collected from streams in the Intrenchment Creek, Peachtree Creek, Proctor Creek, and South River watersheds (basins with CSOs), a number markedly higher than the number detected in water samples from the control basins (watersheds without CSOs). Several compounds known to disrupt the endocrine systems of aquatic biota were among the compounds detected. The median numbers of OWICs detected in base-flow samples from the control basins ranged from 3 to 4 and 7 to 9 in stormflow samples, while the median in base-flow samples from the four CSO-affected watersheds ranged from 4 to 16 and 11 to 19 in stormflow samples. The detection frequencies and concentrations of OWICs in water samples varied depending on flow conditions during sample collection; however, regardless of flow condition, the total OWICs concentrations were strongly related to the numbers of OWICs detected in these samples. In addition, the median number of detectable OWICs and total OWIC concentrations increased linearly with increasing impervious area and stream flashiness (flashiness indicates the rapidity with which streamflow responds to high rainfall amounts). Four compounds-tris(2-butoxyethyl) phosphate (TBEP), tris(2-chloroethyl) phosphate (TCEP), bromacil, and cholesterol-were detected at concentrations greater than study reporting levels in at least 45 percent of all samples collected during the study. On a broad scale, the seasonal distributions of OWICs detected in samples collected during the study period were consistent with use patterns or urban activity, but were markedly different and more variable within individual watersheds. Seven of the nine OWIC classes were detected with greater frequency in base-flow samples from sites downstream from CSOs than from those upstream from CSOs or from control-basin sites; these OWICs also were detected in a greater percentage of base-flow samples from upstream than control-basin sites. Polycyclic aromatic hydrocarbon (PAH) and automotive-use compounds were detected with similar frequency in base-flow samples from all sites. The pesticides and industrial-use compounds were detected with similar frequency in base-flow samples from sites upstream and downstream from CSOs. The compounds 7-acetyl-1,1,3,4,4,6-hexamethyl tetrahydronaphthalene (AHTN), 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethyl cyclopenta-g-2-benzopyran (HHCB), and triclosan were detected with greater frequency in base-flow than in stormflow samples from all sites. The detection frequencies of these three compounds were particularly high in base-flow samples from downstream sites, especially those from the Intrenchment Creek watershed. In stormflow samples, only the industrial-use compounds were detected with similar frequency among samples from control basins, and upstream and downstream sites. Caffeine, camphor, and menthol were detected in a greater percentage of stormflow than base-flow samples from all sites. The disinfectant byproduct bromoform was detected with the highest frequency in base-flow samples from the upstream sites, particularly those from the South River watershed. Typically, compounds in the pesticide class were detected with similar frequency in base-flow and stormflow samples from upstream and downstream sites, although bromacil and carbaryl were the exceptions. Bromacil was detected with greatest frequency in base-flow samples from all sites, but was detected in a larger percentage of base-flow samples from upstream and downstream sites, especially upstream and downstream sites in the Proctor Creek and South River watersheds. Carbaryl, however, was detected in a greater percentage of stormflow samples from all sites. Although collectively the industrial-use compounds were detected in more stormflow than base-flow samples, tetrachloroethene (PCE) was detected in more base-flow than stormflow samples at all sites. More specifically, PCE was detected with the highest frequency in samples from the upstream sites, particularly those from the Proctor Creek watershed. The similarity in the pattern and distribution of OWICs in samples at sites upstream and downstream from known CSO outfalls indicates that CSOs were not the dominant source of OWICs during the study period. Other sources may include non-sewage discharges-both permitted, permitted but out of compliance, and non-permitted, contaminated groundwater from leaking sewer lines or septic systems, sanitary-sewer overflows, or dry-weather runoff from outdoor water use. These OWICs may be better suited for identifying sewage-contaminated groundwater than sewage-contaminated surface water because groundwater is not typically affected by the OWICs that are more common in urban runoff.

Georgia↗

Nutrient attenuation in rivers and streams, Puget Sound Basin, Washington

Nutrients such as nitrogen and phosphorus are important for aquatic ecosystem health. Excessive amounts of nutrients, however, can make aquatic ecosystems harmful for biota because enhanced growth and decay cycles of aquatic algae can reduce dissolved oxygen in the water. In Puget Sound marine waters, low dissolved oxygen concentrations are observed in a number of marine nearshore areas, and nutrients have been identified as a major stressor to the local ecosystem. Delivery of nutrients from major rivers in the Puget Sound Basin to the marine environment can be large. Therefore, it is important to identify factors related to how nutrients are retained (attenuated) within streams and rivers in the Puget Sound Basin. Physical, chemical, and biological factors related to nutrient attenuation were identified through a review of related scientific literature. Numerous empirical modeling approaches for estimating nutrient attenuation in streams and rivers also were compiled, and a subset of these models was applied to the Puget Sound Basin. In particular, models based on the physical characteristics of a river reach (RivR-N model) and on the physical and biological features of a river reach (v f model) were used and compared for the 17 major rivers draining to the Puget Sound. Data on the relative amount of instream attenuation (the fraction of nutrient input removed per kilometer of stream reach) showed some common and general themes. Firstly, headwater reaches throughout the Puget Sound Basin tend to be better than the main stems of the major rivers at attenuating nitrate and orthophosphorus (ortho-P). Secondly, rivers are more efficient at attenuating nitrate than ortho-P, probably because of the close relation between phosphorus and suspended sediment, which was not captured fully in the models. Thirdly, when comparing the RivR-N and v f models for nitrate, physical characteristics of the channel may be more effective predictors of relative nitrate attenuation for main stem reaches, whereas biological factors may be more effective predictors in headwater reaches. These results are explained in terms of four primary factors of attenuation: sinuosity, channel slope, specific discharge, and uptake velocity (v f ) of the reach. A simple scoring procedure based on these four factors showed that reaches where attenuation scores were high had higher relative attenuation of nutrients from the RivR-N and v f models. This attenuation "scorecard" can be used to quickly assess the potential for a given reach to attenuate nutrients. Seasonal relative attenuation at three case studies was greater in summer months (July through September) and much lower and almost constant from January through June. An analysis of relative attenuation across a range of nutrient concentrations showed that, at some point, relative instream attenuation is minimized. For nitrate, relative attenuation reached a minimum value greater than 3 milligrams of nitrogen per liter (mg N/L) during low flow and 1 mg N/L during high flow. For orthophosphate, minimum relative attenuation was observed at about 0.1 milligram of phosphorus per liter (mg P/L) for both low- and high-flow conditions. Generally, the temporal dynamics of nutrient attenuation are dependent on the travel time through a given reach, the proportion of flow in contact with the sediment, and the amount of biological activity. Improved understanding of nutrient attenuation in Puget Sound Basin will benefit from the compilation of more detailed data for specific discharge, channel slope, and channel sinuosity in Puget Sound streams and rivers. Additionally, field studies examining upstream-downstream changes in nutrient load and field-based measurements of v f are needed. From a management perspective, preservation and improvement of instream nutrient attenuation should focus on increasing the travel time through a reach and contact time of water sediment (reactive) surfaces and lowering nutrient concentrations (and loads) to avoid saturation of instream attenuation and increase attenuation efficiency. These goals can be reached by maintaining and restoring channel-flood plain connectivity, maintaining and restoring healthy riparian zones along streams, managing point and nonpoint nutrient loads to streams and rivers, and restoring channel features that promote attenuation such as the addition of woody debris and maintaining pool-riffle morphologies. Many of these management approaches are already being undertaken during projects aimed to restore quality salmon habitat. Therefore, there is a dual benefit to these projects that also may lead to enhanced potential for nitrogen and phosphorus attenuation.

Washington↗

Evaluation of airborne image data for mapping riparian vegetation within the Grand Canyon

This study examined various types of remote-sensing data that have been acquired during a 12-month period over a portion of the Colorado River corridor to determine the type of data and conditions for data acquisition that provide the optimum classification results for mapping riparian vegetation. Issues related to vegetation mapping included time of year, number and positions of wavelength bands, and spatial resolution for data acquisition to produce accurate vegetation maps versus cost of data. Image data considered in the study consisted of scanned color-infrared (CIR) film, digital CIR, and digital multispectral data, whose resolutions from 11 cm (photographic film) to 100 cm (multispectral), that were acquired during the Spring, Summer, and Fall seasons in 2000 for five long-term monitoring sites containing riparian vegetation. Results show that digitally acquired data produce higher and more consistent classification accuracies for mapping vegetation units than do film products. The highest accuracies were obtained from nine-band multispectral data; however, a four-band subset of these data, that did not include short-wave infrared bands, produced comparable mapping results. The four-band subset consisted of the wavelength bands 0.52-0.59 µm, 0.59-0.62 µm, 0.67-0.72 µm, and 0.73-0.85 µm. Use of only three of these bands that simulate digital CIR sensors produced accuracies for several vegetation units that were 10% lower than those obtained using the full multispectral data set. Classification tests using band ratios produced lower accuracies than those using band reflectance for scanned film data; a result attributed to the relatively poor radiometric fidelity maintained by the film scanning process, whereas calibrated multispectral data produced similar classification accuracies using band reflectance and band ratios. This suggests that the intrinsic band reflectance of the vegetation is more important than inter-band reflectance differences in attaining high mapping accuracies. These results also indicate that radiometrically calibrated sensors that record a wide range of radiance produce superior results and that such sensors should be used for monitoring purposes. When texture (spatial variance) at near-infrared wavelength is combined with spectral data in classification, accuracy increased most markedly (20-30%) for the highest resolution (11-cm) CIR film data, but decreased in its effect on accuracy in lower-resolution multi-spectral image data; a result observed in previous studies (Franklin and McDermid 1993, Franklin et al. 2000, 2001). While many classification unit accuracies obtained from the 11-cm film CIR band with texture data were in fact higher than those produced using the 100-cm, nine-band multispectral data with texture, the 11-cm film CIR data produced much lower accuracies than the 100-cm multispectral data for the more sparsely populated vegetation units due to saturation of picture elements during the film scanning process in vegetation units with a high proportion of alluvium. Overall classification accuracies obtained from spectral band and texture data range from 36% to 78% for all databases considered, from 57% to 71% for the 11-cm film CIR data, and from 54% to 78% for the 100-cm multispectral data. Classification results obtained from 20-cm film CIR band and texture data, which were produced by applying a Gaussian filter to the 11-cm film CIR data, showed increases in accuracy due to texture that were similar to those observed using the original 11-cm film CIR data. This suggests that data can be collected at the lower resolution and still retain the added power of vegetation texture. Classification accuracies for the riparian vegetation units examined in this study do not appear to be influenced by season of data acquisition, although data acquired under direct sunlight produced higher overall accuracies than data acquired under overcast conditions. The latter observation, in addition to the importance of band reflectance for classification, implies that data should be acquired near summer solstice when sun elevation and reflectance is highest and when shadows cast by steep canyon walls are minimized.

Arizona↗

Climate-driven shifts in snowpack conditions favorable for wet snow avalanches: Insights from snow cover model simulations

Forecasting wet snow avalanches remains a major challenge due to their temporal and spatial variability. Long-term climate trends are producing more variable and extreme weather, creating conditions outside historical experience, and increasing forecasting uncertainties. To assess how climate change affects the frequency and timing of wet snow avalanches, we examined the long-term conditions that support wet snow formation. Specifically, we ask: Will the onset of wet snow avalanche conditions or season end shift toward the mid century? And will maximum snow height decrease in avalanche starting zones? To address these questions, we analyze long-term snow cover simulations across our study site in Glacier National Park, Montana, located in an intermountain snow climate. We examined trends in simulated snow depth, and timing of liquid water infiltration over the past four decades (1979–2023) using a physically based snow cover model. To force the model with meteorological data, we use the long-term regional hydroclimate reanalysis dataset U.S. Geological Survey CONUS404 with 4 km spatial resolution and 1h temporal resolution. The use of gridded meteorological inputs to drive a one-dimensional point model introduces inherent scale mismatches. To address this limitation, we use multiple grid points within the study area to simulate a more representative, generalized snowpack, and filter the grid points based on elevation to distinguish between upper- and mid-elevation starting zones. Finally, we analyzed shifts in snow depth, melt-out-day, and LWCindex (bulk liquid water content (LWC) normalized by the residual water content). We use snow depth as a proxy for changes in slab thickness, the LWCindexas an indicator of wet snow avalanche conditions, and melt-out date as a measure of shifts in season length. We ran simulations on flat terrain and on north- and south-facing virtual slopes (38°), across mid-elevation (1,600–1,900 m) and upper-elevation (≥1,900 m) sites for the historical dataset as baseline and a Pseudo-Global-Warming (PGW) dataset to identify a warming driven shift. Across all aspects and both elevation bands, PGW forcing produces a consistent reduction in annual maximum snow depth (median shift −0.15 to −0.26 m, p<0.001) over 44 seasons, with the largest reductions on north-facing slopes and a modest but significant strengthening of this reduction over the 44-year simulated record. Melt-out starts 10–14 days earlier under PGW across all aspect–elevation combinations (p<0.001), again most pronounced on north-facing, higher-elevation terrain. The LWCindex threshold-crossing dates likewise shift earlier under PGW, indicating an earlier onset of wet snow avalanche conditions across the domain. Together, these results point to a snowpack that is both thinner and wetter earlier in the season under future warming, with the strongest signal consistently on north-facing slopes at higher elevation. This work lays the groundwork for climate-informed forecasting and mitigation and offers historical context for avalanche operations in a changing climate. Understanding long-term trends can help avalanche professionals interpret current observations, improve wet snow avalanche forecasting, and develop more effective mitigation strategies in a warming climate.

Montana↗

Seasonal and longitudinal variability of benthic macroinvertebrates collected from a series of dam impoundments in a cold-water tailrace

Natural lotic systems follow longitudinal, biophysical gradients as described by the river continuum concept; however, anthropogenic structures such as dams fragment these gradients and alter downstream communities, i.e. the serial discontinuity concept. We investigated the effects of successive impoundments on benthic macroinvertebrate assemblages in the Lower Mountain Fork River (LMFR), a 19-km cold tailwater reach in southeastern Oklahoma fragmented by four dams. Cold releases from Broken Bow Dam maintain year-round trout habitat but substantially modify thermal regimes and flow patterns relative to natural warmwater systems. From June 2016 through May 2017, we sampled 12 sites monthly, which were divided into three zones with 4 sites per zone: below the spillway, below the powerhouse discharge and Old Park dam, and below the re-regulation dam. We quantified abundance, taxa richness, percent Ephemeroptera-Plecoptera-Trichoptera (%EPT), diversity indices (e.g. Shannon and Simpson), functional feeding groups, and community structure using nMDS, ANOSIM, and SIMPER analyses. We collected 19,194 individuals representing 51 taxa. Assemblages were dominated by Trichoptera, Diptera, Isopoda, and Ephemeroptera, with collector/filterers comprising the most abundant functional group. Community structure differed significantly among dam-influenced reaches (ANOSIM global R = 0.521, p < 0.01). Sites nearest the spillway were characterized by high abundance but reduced diversity and low %EPT, driven by dominance of cold-tolerant taxa, including Simulium , Isopoda, and Amphipoda. In contrast, downstream sites exhibited greater taxa richness, higher diversity, and increased %EPT values, suggesting partial recovery along the longitudinal gradient. Seasonal variation was evident, with highest abundance in winter and peak diversity in spring. Our findings support the serial discontinuity concept, demonstrating that hypolimnetic releases and successive impoundments create spatially structured assemblages dominated by cold-tolerant taxa near dams, with gradual biophysical adjustment downstream. Despite substantial alteration, macroinvertebrate communities exhibited resilience and partial convergence toward regional warmwater assemblages at increasing distances from cold-water discharge.

Oklahoma↗

Investigation of plastic debris ingestion by four species of sea turtles collected as bycatch in pelagic Pacific longline fisheries

Ingestion of marine debris is an established threat to sea turtles. The amount, type, color and location of ingested plastics in the gastrointestinal tracts of 55 sea turtles from Pacific longline fisheries from 2012 to 2016 were quantified, and compared across species, turtle length, body condition, sex, capture location, season and year. Six approaches for quantifying amounts of ingested plastic strongly correlated with one another and included: number of pieces, mass, volume and surface area of plastics, ratio of plastic mass to body mass, and percentage of the mass of gut contents consisting of plastic. All olive ridley (n = 37), 90% of green (n = 10), 80% of loggerhead (n = 5) and 0% of leatherback (n = 3) turtles had ingested plastic; green turtles ingested significantly more than olive ridleys. Most debris was in the large intestines. No adverse health impacts (intestinal lesions, blockage, or poor body condition) due directly to plastic ingestion were noted.

Marine Pollution Bulletin↗

Native Prairie Adaptive Management: a multi region adaptive approach to invasive plant management on Fish and Wildlife Service owned native prairies

Much of the native prairie managed by the U.S. Fish and Wildlife Service (FWS) in the Prairie Pothole Region (PPR) of the northern Great Plains is extensively invaded by the introduced cool-season grasses, smooth brome ( Bromus inermis ) and Kentucky bluegrass ( Poa pratensis ). Management to suppress these invasive plants has had poor to inconsistent success. The central challenge to managers is selecting appropriate management actions in the face of biological and environmental uncertainties. In partnership with the FWS, the U.S. Geological Survey (USGS) developed an adaptive decision support framework to assist managers in selecting management actions under uncertainty and maximizing learning from management outcomes. This joint partnership is known as the Native Prairie Adaptive Management (NPAM) initiative. The NPAM decision framework is built around practical constraints faced by FWS refuge managers and includes identification of the management objective and strategies, analysis of uncertainty and construction of competing decision models, monitoring, and mechanisms for model feedback and decision selection. Nineteen FWS field stations, spanning four states of the PPR, have participated in the initiative. These FWS cooperators share a common management objective, available management strategies, and biological uncertainties. Though the scope is broad, the initiative interfaces with individual land managers who provide site-specific information and receive updated decision guidance that incorporates understanding gained from the collective experience of all cooperators. We describe the technical components of this approach, how the components integrate and inform each other, how data feedback from individual cooperators serves to reduce uncertainty across the whole region, and how a successful adaptive management project is coordinated and maintained on a large scale. During an initial scoping workshop, FWS cooperators developed a consensus management objective: increase the composition of native grasses and forbs on native sod while minimizing cost. Cooperators agreed that decision guidance should be provided annually and should account for local, real-time vegetation conditions observed on the ground. Over the course of development, two prototypes of the decision framework were considered. The final framework recognized four alternative actions that managers could take in any given year: (1) Graze—targeted use of grazing ungulates to achieve defoliation, (2) Burn—application of prescribed fire as the single form of defoliation, (3) Burn/Graze—a combination treatment, and (4) Rest—no action. The study area included northern mixed-grass and tallgrass prairie. Native vegetation in mixed–grass prairie has a strong cool-season component and thus the dominant native species have a phenology similar to that of smooth brome and Kentucky bluegrass, making management of those species challenging. In contrast, tallgrass prairie has a strong warm-season native component, leading to an existence of cool-season windows, periods of time in the fall and spring when cool‐season invasive grass species are actively growing and vulnerable to damage via select management actions, but warm‐season grass species are not active and are thus less susceptible to damage via the same actions. This dichotomy between prairie types necessitated the development of separate but parallel decision support systems for mixed-grass and tallgrass biomes. Management units are parcels of native prairie that receive a single management treatment at any one time over their entire extent. At any particular time, the vegetation state of each management unit is characterized by the amount of cover of native grasses and forbs and the type of invasive grass that is dominant. In addition, each unit has a defoliation state which reflects the number of years since the last defoliation event and an index to how intensively the unit was managed during the previous 7 years. State-transition models are used to predict the state of a management unit in year t+1 from its state in year t and a prescribed management action that was applied between the two monitoring events. Alternative models are built around key uncertainties that make choice of a management action difficult. Three uncertainties revolve around whether the effect of management actions depends on (1) type of dominant invader, (2) past defoliation history, and (3) level of invasion. Two additional uncertainties are considered when choosing a management action for tallgrass units: (4) the effectiveness of grazing within the cool-season window as a surrogate for burning when smooth brome is the dominant invader, and (5) the differential effect of active management outside the window as compared to rest. Because data on the probability of transitioning from one state to another under the various models were lacking, expert opinion and elicitation were used to parameterize the models. In addition, cooperators participated in elicitation exercises to extract their beliefs regarding the value of having native prairie compared to the cost of achieving it. Quantifying the subjective expression of utility in this way allowed for mathematical representation of the management objective into an objective function. By maximizing the objective function, cumulative utility is maximized, leading to the identification of a sequence of decisions that will achieve the management objective. The NPAM system adopted a vegetation monitoring protocol that was rapid, inexpensive, and familiar to many of the cooperators. The monitoring protocol served three purposes: (1) determining current vegetation and defoliation states of each unit, (2) evaluating progress toward the management objective, and (3) assessing predictive performance of the alternative models. The management year runs from September 1 to August 31. Management can be applied anytime during that period and monitoring takes places from late June to mid-August. Cooperators enter vegetation data and management information into a centralized database by August 25 of each year. Given the current state of the system (vegetation and defoliation states) and the current understanding of the system (or the belief state), identifying the current best management decision is a matter of looking up the combination (that is, system state and belief state) in the appropriate (mixed-grass or tallgrass) optimal decision table. Given complete uncertainty at the outset of decision-making, initial assignment of equal belief weights to each model was believed reasonable. The decisions in the optimal decision table that correspond to the current belief state constitute the current optimal decision policy. By August 31 of each year, individual cooperators are provided with a recommended management action for each of their management units for the upcoming management year. Upon receiving the management recommendations for their units, managers consider the recommendation, along with other relevant information, and at some point during the year one of the management alternatives is carried out. This iterative cycle of making and implementing a management decision, predicting the response, monitoring the outcome, comparing predicted and observed outcomes, updating model weights, and recommending a management action for the next cycle is expected to result in an accumulation of weight on a representative model of system dynamics, thereby increasing understanding needed to effectively manage native prairies. The NPAM system is now entering its second full year of complete operation, and represents one of only a few fully implemented applications of adaptive management within the U.S. Fish and Wildlife Service. NPAM is truly unique in that it originated from the ground up as a result of the leadership and steadfastness of several refuge biologists and managers confronted with a common problem. These biologists recognized that working together across a large landscape presented perhaps the best opportunity for halting and reversing the invasion of native grasslands by non-native cool-season grasses. Importantly, the NPAM system encapsulates the collective thinking and experience of tens if not hundreds of individuals who have battled this vexing problem for much of their careers. The NPAM initiative is rooted in principles of adaptive management, thereby affording the opportunity for grassland managers to pursue management objectives while acquiring information to reduce uncertainty and improve future management. The project introduced a number of technical innovations that will serve as templates for conservation efforts throughout and beyond the U.S. Fish and Wildlife Service. First, NPAM is an on-the-ground implementation of active adaptive management—possibly the first of its kind in conservation management—in which recommended management actions result from a prospective analysis of future learning (Williams, 1996). Second, by the use of dynamic optimization, NPAM demonstrates how decisions can be made that take into account possible future transitions of the system. Third, NPAM demonstrates how models of partial controllability are an effective means of accommodating unpredictable circumstances that cause a manager to follow a different course than was intended. Finally, the database developed for NPAM is an unparalleled system that enables the rapid integration of data from the field for the generation of ‘just-in-time’ management recommendations. In all, NPAM provides an example of how a science-management partnership can be forged to achieve large-scale conservation objectives.

Minnesota;Montana;North Dakota;South Dakota↗

Migration And wintering areas Of Glaucous-winged Gulls From south-central Alaska

We used satellite telemetry to investigate the migration patterns and wintering areas of Glaucous-winged Gulls (Larus glaucescens) from Middleton Island, Alaska, where this species' population increased tenfold from the 1970s to the 1990s. Fall migration spanned 11 weeks, including numerous stopovers en route, apparently for feeding. Spring migration from wintering sites to Middleton Island was shorter (4 weeks) and more direct. One juvenile spent several months in southern Prince William Sound. An adult spent several months near Craig, southeast Alaska, while three others overwintered in southern British Columbia. For all four wintering adults use of refuse-disposal sites was evident or strongly suggested. Commensalism with humans may have contributed to the increase on Middleton, but a strong case can also be made for a competing explanation-regional recruitment of gulls to high-quality nesting habitat in Alaska created after the earthquake of 1964. An analysis of band returns reveals broad overlap in the wintering grounds of gulls from different Alaska colonies and of gulls banded on the west coast from British Columbia to California. The seasonal movement of many gulls from Alaska is decidedly migratory, whereas gulls from British Columbia, Washington, and Oregon disperse locally in winter.

Alaska↗

Cytochrome P4501A biomarker indication of oil exposure in harlequin ducks up to 20 years after the Exxon Valdez oil spill

Hydrocarbon-inducible cytochrome P4501A (CYP1A) expression was measured, as ethoxyresorufin- O -deethylase (EROD) activity, in livers of wintering harlequin ducks ( Histrionicus histrionicus ) captured in areas of Prince William Sound, Alaska, USA, oiled by the 1989 Exxon Valdez spill and in birds from nearby unoiled areas, during 2005 to 2009 (up to 20 years following the spill). The present work repeated studies conducted in 1998 that demonstrated that in harlequin ducks using areas that received Exxon Valdez oil, EROD activity was elevated nearly a decade after the spill. The present findings strongly supported the conclusion that average levels of hepatic EROD activity were higher in ducks from oiled areas than those from unoiled areas during 2005 to 2009. This result was consistent across four sampling periods; furthermore, results generated from two independent laboratories using paired liver samples from one of the sampling periods were similar. The EROD activity did not vary in relation to age, sex, or body mass of individuals, nor did it vary strongly by season in birds collected early and late in the winter of 2006 to 2007, indicating that these factors did not confound inferences about observed differences between oiled and unoiled areas. We interpret these results to indicate that harlequin ducks continued to be exposed to residual Exxon Valdez oil up to 20 years after the original spill. This adds to a growing body of literature suggesting that oil spills have the potential to affect wildlife for much longer time frames than previously assumed.

Alaska↗

Degradation of the disease-associated prion protein by a serine protease from lichens

The disease-associated prion protein (PrP(TSE)), the probable etiological agent of the transmissible spongiform encephalopathies (TSEs), is resistant to degradation and can persist in the environment. Lichens, mutualistic symbioses containing fungi, algae, bacteria and occasionally cyanobacteria, are ubiquitous in the environment and have evolved unique biological activities allowing their survival in challenging ecological niches. We investigated PrP(TSE) inactivation by lichens and found acetone extracts of three lichen species (Parmelia sulcata, Cladonia rangiferina and Lobaria pulmonaria) have the ability to degrade prion protein (PrP) from TSE-infected hamsters, mice and deer. Immunoblots measuring PrP levels and protein misfolding cyclic amplification indicated at least two logs of reductions in PrP(TSE). Degradative activity was not found in closely related lichen species or in algae or a cyanobacterium that inhabit lichens. Degradation was blocked by Pefabloc SC, a serine protease inhibitor, but not inhibitors of other proteases or enzymes. Additionally, we found that PrP levels in PrP(TSE)-enriched preps or infected brain homogenates are also reduced following exposure to freshly-collected P. sulcata or an aqueous extract of the lichen. Our findings indicate that these lichen extracts efficiently degrade PrP(TSE) and suggest that some lichens could have potential to inactivate TSE infectivity on the landscape or be a source for agents to degrade prions. Further work to clone and characterize the protease, assess its effect on TSE infectivity and determine which organism or organisms present in lichens produce or influence the protease activity is warranted.

PLoS ONE↗

Comparing imidacloprid, clothianidin, and azoxystrobin runoff from lettuce fields using a soil drench or treated seeds in the Salinas Valley, California

Neonicotinoid insecticide use has increased over the last decade, including as agricultural seed treatments (application of chemical in a coating to the seed prior to planting). In California, multiple crops, including lettuce, can be grown using neonicotinoid treated seeds or receive a direct neonicotinoid soil application (drenching) at planting. Using research plots, this study compared pesticide runoff in four treatments: (1) imidacloprid seed treatment; (2) clothianidin seed treatment; (3) imidacloprid drench and an azoxystrobin seed treatment; and (4) a control with no pesticidal treatment. Neonicotinoid and azoxystrobin concentrations were measured in surface water runoff during six irrigations events in the 2020 growing seasons. Results showed runoff concentrations up to 1308 (±1200) ng L −1 for imidacloprid drench treatment, 431 (±100) ng L −1 for clothianidin seed treatment, 135 (±60) ng L −1 for imidacloprid seed treatment, 13 (±10) ng L −1 for azoxystrobin seed treatment (treatments averaged). The percent of applied mass in runoff over the entire sampling period varied by compound; the imidacloprid seed treatment and drench were similar (0.015 and 0.019%, respectively) to the clothianidin seed treatment (0.036%) while the azoxystrobin seed treatment was much higher (15%). Although the proportion of imidacloprid in runoff was similar for imidacloprid treatments, the mass applied during soil drench was > 4x the amount applied from the imidacloprid seed treatment. Surface soils were collected before planting and at the end of the trial. The neonicotinoids were detected in soil throughout the study and average maximum concentrations were 9–13 ng g −1 ; azoxystrobin was detected in only two soils at concentrations up to 0.57 ng g −1 . These results elucidate the comparative mass runoff resulting from planting treated seed and soil drench applications and highlight the value of additional work to characterize off-site transport from the many commodities that may be utilizing treated seeds.

California↗

Near real-time detection of winter cover crop termination using harmonized Landsat and Sentinel-2 (HLS) to support ecosystem assessment

Cover crops are planted to reduce soil erosion, increase soil fertility, and improve watershed management. In the Delmarva Peninsula of the eastern United States, winter cover crops are essential for reducing nutrient and sediment losses from farmland. Cost-share programs have been created to incentivize cover crops to achieve conservation objectives. This program required that cover crops be planted and terminated within a specified time window. Usually, farmers report cover crop termination dates for each enrolled field (∼28,000 per year), and conservation district staff confirm the report with field visits within two weeks of termination. This verification process is labor-intensive and time-consuming and became restricted in 2020–2021 due to the COVID-19 pandemic. This study used Harmonized Landsat and Sentinel-2 (HLS, version 2.0) time-series data and the within-season termination (WIST) algorithm to detect cover crop termination dates over Maryland and the Delmarva Peninsula. The estimated remote sensing termination dates were compared to roadside surveys and to farmer-reported termination dates from the Maryland Department of Agriculture database for the 2020–2021 cover crop season. The results show that the WIST algorithm using HLS detected 94% of terminations (statuses) for the enrolled fields (n = 28,190). Among the detected terminations, about 49%, 72%, 84%, and 90% of remote sensing detected termination dates were within one, two, three, and four weeks of agreement to farmer-reported dates, respectively. A real-time simulation showed that the termination dates could be detected one week after termination operation using routinely available HLS data, and termination dates detected after mid-May are more reliable than those from early spring when the Normalized Difference Vegetation Index (NDVI) was low. We conclude that HLS imagery and the WIST algorithm provide a fast and consistent approach for generating near-real-time cover crop termination maps over large areas, which can be used to support cost-share program verification.

Maryland↗

Intercontinental reassortment and genomic variation of low pathogenic avian influenza viruses isolated from northern pintails ( Anas acuta ) in Alaska: examining the evidence through space and time

Migration and population genetic data for northern pintails ( Anas acuta ) and phylogenetic analysis of low pathogenic avian influenza (LPAI) viruses from this host in Alaska suggest that northern pintails are involved in ongoing intercontinental transmission of avian influenza. Here, we further refine this conclusion through phylogenetic analyses which demonstrate that detection of foreign lineage gene segments is spatially dependent and consistent through time. Our results show detection of foreign lineage gene segments to be most likely at sample locations on the Alaska Peninsula and least likely along the Southern Alaska Coast. Asian lineages detected at four gene segments persisted across years, suggesting maintenance in avian hosts that migrate to Alaska each year from Asia or in hosts that remain in Alaska throughout the year. Alternatively, live viruses may persist in the environment and re-infect birds in subsequent seasons.

Alaska↗

Analysis and modeling of long‐term stream temperatures on the Steamboat Creek Basin, Oregon: Implications for land use and fish habitat

Steamboat Creek basin is an important source of timber and provides crucial spawning and rearing habitat for anadromous steelhead trout ( Oncorhynchus mykiss ). Because stream temperatures are near the upper limit of tolerance for the survival of juvenile steelhead, the possible long‐term effect of clear‐cut logging on stream temperatures was assessed. Twenty‐year (1969–1989) records of summer stream temperature and flow from four tributaries and two reaches of Steamboat Creek and Boulder Creek (a nearby unlogged watershed) were analyzed. Logging records for the Steamboat Creek basin and air temperature records also were used in the analysis. A time‐series model of the components of stream temperature (seasonal cycle of solar radiation, air temperature, streamflow, an autoregressive term of order 1, and a linear trend variable) was fitted to the water‐temperature data. The linear trend variable was significant in all the fitted models except Bend Creek (a tributary fed by cool ground‐water discharge) and Boulder Creek. Because no trends in either climate (i.e., air temperature) or streamflow were found in the data, the trend variable was associated with the pre‐1969 loss and subsequent regrowth of riparian vegetation and shading canopies.

Oregon↗

Water-quality assessment of Cache Creek, Yolo, Lake, and Colusa counties, California

Cache Creek and its tributaries from Clear Lake to Yolo Bypass have been the subject of quality and quantity of water studies by several governmental agencies since the early 1900's. Water-quality data from these studies showed that water in the basin is of good quality for most of the beneficial uses defined by the California State Water Resources Control Board. Concentrations of dissolved constituents are substantially higher in the water in the two largest tributaries than in Cache Creek. Seasonal variations in dissolved constituents are also greater in the tributaries than in Cache Creek. Clear Lake has a major effect on water quality, resulting in little seasonal fluctuation in water quality in Cache Creek. Excessive voron and suspended-sediment concentrations are the greatest water-quality problems, according to existing data. Both of these problems are from natural sources. Water-quality monitoring is presently being conducted monthly at four sites by the California Department of Water Resurces and at several other sites by other agencies. Modifications in current monitoring are proposed to gain further information on diel dissolved-oxygen cycles, pesticides, and biological constituents that may adversely affect beneficial uses. (USGS)

Open-File Report↗

Post-breeding migration of Dutch-breeding black-tailed godwits: timing, routes, use of stopovers, and nonbreeding destinations

Conservation of long-distance migratory shorebirds is complex because these species use habitats spread across continents and hemispheres, making identification of critical habitats and potential bottlenecks in the annual cycle especially difficult. The population of Black-tailed Godwits that breeds in Western Europe, Limosa limosa limosa , has declined precipitously over the past few decades. Despite significant efforts to identify the root causes of this decline, much remains unclear. To better understand the migratory timing, use of stopover and nonbreeding sites, and the potential impact of breeding success on these parameters, we attached 15 Argos satellite transmitters and 10 geolocation tracking devices to adult godwits nearing completion of incubation at breeding sites in southwest Friesland, The Netherlands during the spring of 2009. We successfully tracked 16 adult godwits for their entire southward migration and two others for part of it. Three migration patterns and four regions of use were apparent. Most godwits left their breeding sites and proceeded south directly to stopover sites in the Mediterranean &mdash; e.g. Spain, Portugal, and Morocco &mdash; before flying on to non-breeding sites in West Africa. Other individuals spent the entire nonbreeding season in the Mediterranean. A third pattern included a few individuals that flew nonstop from their Dutch breeding sites to nonbreeding sites in West Africa. Tracking data from this study will be immediately useful for conservation efforts focused on preserving the dispersed network of sites used by godwits during their southward migration.

Friesland↗

Small mammal populations in Maryland meadows during four years of herbicide (brominal®) applications

The herbicide Brominal® was applied at the recommended rate to one plot in each of three paired 0.6-ha plots; the other three plots were used as controls. Plots were sprayed once in the fall of 1988 and 1989 and twice in the spring of 1990 and 1991. Small mammals were trapped three times during each activity season (April-October) to obtain population estimates before and after spraying and in the spring preceding fall spraying or the fall following spring spraying. Population estimates immediately after spraying gave no evidence of direct mortality. By 1991, dicot vegetation on treated plots was suppressed and mean numbers of meadow voles (Microtus pennsylvanicus) were less than on control plots. Because meadow voles favor dicots over monocots in their diet, reduced availability of dicots may have been related to the smaller vole population estimates. Species diversity of small mammals was negatively correlated with size of vole populations, but was not different between treated and control plots. Brominal apparently induced opaque corneas in nine voles. The condition was found in two voles too small to have been conceived at the time of the last previous spray nearly 8 months earlier, suggesting exposure to residue alone.

Environmental Toxicology and Chemistry↗