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At least 649 records · Page 36Linked to original sources

USGS2020

Vegetation‐groundwater dynamics at a former uranium mill site following invasion of a biocontrol agent: A time series analysis of Landsat normalized difference vegetation index data

Because groundwater recharge in dry regions is generally low, arid and semiarid environments have been considered well‐suited for long‐term isolation of hazardous materials (e.g., radioactive waste). In these dry regions, water lost (transpired) by plants and evaporated from the soil surface, collectively termed evapotranspiration (ET), is usually the primary discharge component in the water balance. Therefore, vegetation can potentially affect groundwater flow and contaminant transport at waste disposal sites. We studied vegetation health and ET dynamics at a Uranium Mill Tailings Radiation Control Act (UMTRCA) disposal site in Shiprock, New Mexico, where a floodplain alluvial aquifer was contaminated by mill effluent. Vegetation on the floodplain was predominantly deep‐rooted, non‐native tamarisk shrubs ( Tamarix sp.). After the introduction of the tamarisk beetle ( Diorhabda sp.) as a biocontrol agent, the health of the invasive tamarisk on the Shiprock floodplain declined. We used Landsat normalized difference vegetation index (NDVI) data to measure greenness and a remote sensing algorithm to estimate landscape‐scale ET along the floodplain of the UMTRCA site in Shiprock prior to (2000–2009) and after (2010–2018) beetle establishment. Using groundwater level data collected from 2011 to 2014, we also assessed the role of ET in explaining seasonal variations in depth to water of the floodplain. Growing season scaled NDVI decreased 30% ( p < .001), while ET decreased 26% from the pre‐ to post‐beetle period and seasonal ET estimates were significantly correlated with groundwater levels from 2011 to 2014 ( r 2 = .71; p = .009). Tamarisk greenness (a proxy for health) was significantly affected by Diorhabda but has partially recovered since 2012. Despite this, increased ET demand in the summer/fall period might reduce contaminant transport to the San Juan River during this period.

New Mexico↗

USGS2004

Rehabilitation of a lignite mine-disturbed area in the Indian Desert

Extensive lignite mining in the Indian (Thar) Desert commenced within the past decade. Accompanying extraction of this valuable resource there have been visible, important environmental impacts. The resultant land degradation has prompted concern from both public and regulatory bodies. This research assesses the success of rehabilitation plans implemented to revegetate a lignite mine-disturbed area, near the village of Giral in western Rajasthan State. Rehabilitation success was achieved within the environmental constraints of this northwest Indian hot-desert ecosystem using a combination of: (1) backfilling (abandoned pits) with minespoil and of covering the backfilled-surfaces with fresh topsoil to a thickness of about 0??30 m; (2) use of micro-catchment rainwater harvesting (MCWH) technique; (3) soil profile modification approaches; (4) plant establishment methodologies; and (5) the selection of appropriate germplasm material (trees, shrubs and grasses). Preliminary results indicate that the resulting vegetative cover will be capable of self-perpetuation under natural conditions while at the same time meeting the land-use requirements of the local people. The minespoil is alkaline in nature and has high electrical conductance. The average content of organic carbon, N, P and K is lower than in the regional topsoil. However, the concentration of Ca, Mg, Na and total S in the minespoil is much higher than in the topsoil. Further, the spoil material has no biological activity. Enhanced plant growth was achieved in MCWH plots, compared to control plots, where minespoil moisture storage was improved by 18-43 per cent. The rehabilitation protocol used at the site appears to have been successful because, in addition to the planted species, desirable native invasive species have become established. This study developed methods for the rehabilitation of lignite mine-disturbed areas and has also resulted in an understanding of rehabilitation processes in arid regions with an emphasis on the long-term monitoring of rehabilitation success. ?? 2004 John Wiley and Sons, Ltd.

Land Degradation and Development↗

USGS2020

Ultra‐high‐resolution mapping of biocrusts with Unmanned Aerial Systems

Biological soil crusts (biocrusts) occur in drylands globally where they support ecosystem functioning by increasing soil stability, reducing dust emissions and modifying soil resource availability (e.g. water, nutrients). Determining biocrust condition and extent across landscapes continues to present considerable challenges to scientists and land managers. Biocrusts grow in patches, cover vast expanses of rugged terrain and are vulnerable to physical disturbance associated with ground‐based mapping techniques. As such, remote sensing offers promising opportunities to map and monitor biocrusts. While satellite‐based remote sensing has been used to detect biocrusts at relatively large spatial scales, few studies have used high‐resolution imagery from Unmanned Aerial Systems (UAS) to map fine‐scale patterns of biocrusts. We collected sub‐centimeter, true color 3‐band imagery at 10 plots in sagebrush and pinyon‐juniper woodland communities in a semiarid ecosystem in the southwestern US and used object‐based image analysis (OBIA) to segment and classify the imagery into maps of light and dark biocrusts, bare soil, rock and various vegetation covers. We used field data to validate the classifications and assessed the spatial distribution and configuration of different classes using fragmentation metrics. Map accuracies ranged from 46 to 77% (average 65%) and were higher in pinyon‐juniper (average 70%) versus sagebrush (average 60%) plots. Biocrust classes showed generally high accuracies at both pinyon‐juniper plots (average dark crust = 70%; light crust = 80%) and sagebrush plots (average dark crust = 69%; light crust = 77%). Point cloud density, sun elevation and spectral confusion between vegetation cover explained some differences in accuracy across plots. Spatial analyses of classified maps showed that biocrust patches in pinyon‐juniper plots were generally larger, more aggregated and contiguous than in sagebrush plots. Pinyon‐juniper plots also had greater patch richness and a lower Shannon evenness index than sagebrush plots, suggesting greater soil cover heterogeneity in this plant community type.

Utah↗

USGS2013

A unified assessment of hydrological and biogeochemical responses in research watersheds in Eastern Puerto Rico using runoff-concentration relations

An examination of the relation between runoff rate, R , and concentration, C , of twelve major constituents in four small watersheds in eastern Puerto Rico demonstrates a consistent pattern of responses. For solutes that are not substantially bioactive (alkalinity, silica, calcium, magnesium, sodium, and chloride), the log( R )–log( C ) relation is almost linear and can be described as a weighted average of two sources, bedrock weathering and atmospheric deposition. The slope of the relation for each solute depends on the respective source contributions to the total river load. If a solute were strictly derived from bedrock weathering, the slope would be −0.3 to −0.4, whereas if strictly derived from atmospheric deposition, the slope would be approximately −0.1. The bioactive constituents (dissolved organic carbon, nitrate, sulfate, and potassium), which are recycled by plants and concentrated in shallow soil, demonstrate nearly flat or downward-arched log( R )–log( C ) relations. The peak of the arch represents a transition from dominantly soil-matrix flow to near-surface macropore flow, and finally to overland flow. At highest observed R (80 to >90 mm/h), essentially all reactive surfaces have become wetted, and the input rate of C becomes independent of R (log( R )–log( C ) slope of –1). The highest R are tenfold greater than any previous study. Slight clockwise hysteresis for many solutes in the rivers with riparian zones or substantial hyporheic flows indicates that these settings may act as mixing end-members. Particulate constituents (suspended sediment and particulate organic carbon) show slight clockwise hysteresis, indicating mobilization of stored sediment during rising stage.

Aquatic Geochemistry↗

USGS2004

Development and Application of Pyrolysis Gas Chromatography/Mass Spectrometry for the Analysis of Bound Trinitrotoluene Residues in Soil

TNT (trinitrotoluene) is a contaminant of global environmental significance, yet determining its environmental fate has posed longstanding challenges. To date, only differential extraction-based approaches have been able to determine the presence of covalently bound, reduced forms of TNT in field soils. Here, we employed thermal elution, pyrolysis, and gas chromatography/mass spectrometry (GC/MS) to distinguish between covalently bound and noncovalently bound reduced forms of TNT in soil. Model soil organic matter-based matrixes were used to develop an assay in which noncovalently bound (monomeric) aminodinitrotoluene (ADNT) and diaminonitrotoluene (DANT) were desorbed from the matrix and analyzed at a lower temperature than covalently bound forms of these same compounds. A thermal desorption technique, evolved gas analysis, was initially employed to differentiate between covalently bound and added 15N-labeled monomeric compounds. A refined thermal elution procedure, termed "double-shot analysis" (DSA), allowed a sample to be sequentially analyzed in two phases. In phase 1, all of an added 15N-labeled monomeric contaminant was eluted from the sample at relatively low temperature. In phase 2 during high-temperature pyrolysis, the remaining covalently bound contaminants were detected. DSA analysis of soil from the Louisiana Army Ammunition Plant (LAAP; ???5000 ppm TNT) revealed the presence of DANT, ADNT, and TNT. After scrutinizing the DSA data and comparing them to results from solvent-extracted and base/acid-hydrolyzed LAAP soil, we concluded that the TNT was a noncovalently bound "carryover" from phase 1. Thus, the pyrolysis-GC/MS technique successfully defined covalently bound pools of ADNT and DANT in the field soil sample.

Environmental Science & Technology↗

USGS2008

Evaluation of MODIS NDVI and NDWI for vegetation drought monitoring using Oklahoma Mesonet soil moisture data

The evaluation of the relationship between satellite-derived vegetation indices (normalized difference vegetation index and normalized difference water index) and soil moisture improves our understanding of how these indices respond to soil moisture fluctuations. Soil moisture deficits are ultimately tied to drought stress on plants. The diverse terrain and climate of Oklahoma, the extensive soil moisture network of the Oklahoma Mesonet, and satellite-derived indices from the Moderate Resolution Imaging Spectroradiometer (MODIS) provided an opportunity to study correlations between soil moisture and vegetation indices over the 2002-2006 growing seasons. Results showed that the correlation between both indices and the fractional water index (FWI) was highly dependent on land cover heterogeneity and soil type. Sites surrounded by relatively homogeneous vegetation cover with silt loam soils had the highest correlation between the FWI and both vegetation-related indices (r???0.73), while sites with heterogeneous vegetation cover and loam soils had the lowest correlation (r???0.22). Copyright 2008 by the American Geophysical Union.

Geophysical Research Letters↗

USGS2025

Ecological acclimation: A framework to integrate fast and slow responses to climate change

Ecological responses to climate change occur across vastly different time-scales, from minutes for physiological plasticity to decades or centuries for community turnover and evolutionary adaptation. Accurately predicting the range of ecosystem trajectories will require models that incorporate both fast processes that may keep pace with climate change and slower ones likely to lag behind and generate disequilibrium dynamics. However, the knowledge necessary for this integration is currently fragmented across disciplines. We develop ‘ecological acclimation’ as a unifying framework to emphasize the similarity of dynamics driven by processes operating on dramatically different time-scales and levels of biological organization. The framework focuses on ecoclimate sensitivities, measured as the change in an ecological response variable per unit of climate change. Acclimation processes acting at different time-scales cause these sensitivities to shift in magnitude and even direction over time. We highlight shifting ecoclimate sensitivities in case studies from diverse ecosystems, including terrestrial plant communities, coral reefs and soil microbiomes. Models predicting future ecosystem states inevitably make assumptions about acclimation processes; these assumptions must be explicit for users to evaluate whether a model is appropriate for a given forecast horizon. Similarly, decision frameworks that clearly account for multiple acclimation processes and their distinct time-scales will help natural resource managers plan for ecological impacts of climate change from years to many decades into the future. We outline a synthetic research programme focused on the time-scales of ecological acclimation to reduce uncertainty in ecological forecasts.

Functional Ecology↗

USGS2013

Fine-scale hydrologic modeling for regional landscape applications: the California Basin Characterization Model development and performance

Introduction Resource managers need spatially explicit models of hydrologic response to changes in key climatic drivers across variable landscape conditions. We demonstrate the utility of a Basin Characterization Model for California (CA-BCM) to integrate high-resolution data on physical watershed characteristics with historical or projected climate data to predict watershed-specific hydrologic responses. Methods The CA-BCM applies a monthly regional water-balance model to simulate hydrologic responses to climate at the spatial resolution of a 270-m grid. The model has been calibrated using a total of 159 relatively unimpaired watersheds for the California region. Results As a result of calibration, predicted basin discharge closely matches measured data for validation watersheds. The CA-BCM recharge and runoff estimates, combined with estimates of snowpack and timing of snowmelt, provide a basis for assessing variations in water availability. Another important output variable, climatic water deficit , integrates the combined effects of temperature and rainfall on site-specific soil moisture, a factor that plants may respond to more directly than air temperature and precipitation alone. Model outputs are calculated for each grid cell, allowing results to be summarized for a variety of planning units including hillslopes, watersheds, ecoregions, or political boundaries. Conclusions The ability to confidently calculate hydrologic outputs at fine spatial scales provides a new suite of hydrologic predictor variables that can be used for a variety of purposes, such as projections of changes in water availability, environmental demand, or distribution of plants and habitats. Here we present the framework of the CA-BCM model for the California hydrologic region, a test of model performance on 159 watersheds, summary results for the region for the 1981–2010 time period, and changes since the 1951–1980 time period.

California↗

USGS2010

Climate change effects on an endemic-rich edaphic flora: resurveying Robert H. Whittaker's Siskiyou sites (Oregon, USA)

Species with relatively narrow niches, such as plants restricted (endemic) to particular soils, may be especially vulnerable to extinction under a changing climate due to the enhanced difficulty they face in migrating to suitable new sites. To test for community-level effects of climate change, and to compare such effects in a highly endemic-rich flora on unproductive serpentine soils vs. the flora of normal (diorite) soils, in 2007 we resampled as closely as possible 108 sites originally studied by ecologist Robert H. Whittaker from 1949 to 1951 in the Siskiyou Mountains of southern Oregon, USA. We found sharp declines in herb cover and richness on both serpentine and diorite soils. Declines were strongest in species of northern biogeographic affinity, species endemic to the region (in serpentine communities only), and species endemic to serpentine soils. Consistent with climatic warming, herb communities have shifted from 1949-1951 to 2007 to more closely resemble communities found on xeric (warm, dry) south-facing slopes. The changes found in the Siskiyou herb flora suggest that biotas rich in narrowly distributed endemics may be particularly susceptible to the effects of a warming climate.

Oregon↗

USGS1996

Regional evaluation of evapotranspiration in the Everglades

Understanding the water budget of the Everglades system is crucial to the success of restoration and management strategies. Although the water budget is simple in concept, it is difficult to assess quantitatively. Models used to simulate changes in water levels and vegetation resulting from management strategies need to accurately simulate all components of the water budget. One of the most important components of the Everglades water budget is evapotranspiration (ET). ET is water removed from the surface and soils by direct evaporation and plant transpiration. In South Florida, ET rates may exceed 40 in/yr (inches per year) on the average; during dry years, the ET could exceed rainfall (about 50 in/yr). Thus, most of the water that falls on the land surface as rainfall is returned to the atmosphere by ET. Despite the importance of ET in the Everglades water budget, our knowledge of ET is, at present, only semi-quantitative. Recent advances in instrumentation and measurement techniques have made it possible to continuously measure ET, so that an accurate evaluation of ET in the Everglades can be made.

Florida↗

USGS2006

Potential effects of elevated atmospheric carbon dioxide (CO 2 ) on coastal wetlands

Carbon dioxide (CO 2 ) concentration in the atmosphere has steadily increased from 280 parts per million (ppm) in preindustrial times to 381 ppm today and is predicted by some models to double within the next century. Some of the important pathways whereby changes in atmospheric CO 2 may impact coastal wetlands include changes in temperature, rainfall, and hurricane intensity ( fig. 1 ). Increases in CO 2 can contribute to global warming, which may (1) accelerate sea-level rise through melting of polar ice fields and steric expansion of oceans, (2) alter rainfall patterns and salinity regimes, and (3) change the intensity and frequency of tropical storms and hurricanes. Sea-level rise combined with changes in storm activity may affect erosion and sedimentation rates and patterns in coastal wetlands and maintenance of soil elevations. Feedback loops between plant growth and hydroedaphic conditions also contribute to maintenance of marsh elevations through accumulation of organic matter. Although increasing CO 2 concentration may contribute to global warming and climate changes, it may also have a direct impact on plant growth and development by stimulating photosynthesis or improving water use efficiency. Scientists with the U.S. Geological Survey are examining responses of wetland plants to elevated CO 2 concentration and other factors. This research will lead to a better understanding of future changes in marsh species composition, successional rates and patterns, ecological functioning, and vulnerability to sea-level rise and other global change factors.

Fact Sheet↗

USGS1958

Periodic heat flow in a stratified medium with application to permafrost problems

Solutions to the Fourier heat equation for quasi-steady periodic flow in a stratified semi-infinite medium can be obtained readily by standard methods. The results have wide application to studies of earth-temperature variations induced by diurnal, annual, and other periodic variations in ground surface temperature. Much of the previous work on this subject has been interpreted with reference to the solution for the homogeneous case; and this can be seriously in error when applied to stratified earth materials. One application of the theory is to the important problem of determining the minimum thickness of gravel fill required to maintain the material on which it rests (the subgrade) in a perennially frozen state in permafrost areas. The results indicate that the required fill thickness is quite sensitive to the thermal properties of the subgrade. If a thin layer of material with low thermal contact coefficient, such as spruce logs, is placed between the fill and subgrade, the thickness of fill required to maintain undisturbed permafrost can be greatly reduced. The thermal properties of the soil beneath the layer supporting plant growth can exercise an important influence on the temperature in that layer. This effect, which cannot be explained by studies of the ground surface and the surficial layer, is likely to have important application to plant ecology in the Arctic.

Open-File Report↗

USGS1998

Mississippi Basin Carbon Project science plan

Understanding the carbon cycle is one of the most difficult challenges facing scientists who study the global environment. Lack of understanding of global carbon cycling is perhaps best illustrated by our inability to balance the present-day global CO 2 budget. The amount of CO 2 produced by burning fossil fuels and by deforestation appears to exceed the amount accumulating in the atmosphere and oceans. The carbon needed to balance the CO 2 budget (the so-called "missing" carbon) is probably absorbed by land plants and ultimately deposited in soils and sediments. Increasing evidence points toward the importance of these terrestrial processes in northern temperate latitudes. Thus, efforts to balance the global CO 2 budget focus particular attention on terrestrial carbon uptake in our own North American "backyard." The USGS Mississippi Basin Carbon Project conducts research on the carbon budget in soils and sediments of the Mississippi River basin. The project focuses on the effects of land-use change on carbon storage and transport, nutrient cycles, and erosion and sedimentation throughout the Mississippi River Basin. Particular emphasis is placed on understanding the interactions among changes in erosion, sedimentation, and soil dynamics. The project includes spatial analysis of a wide variety of geographic data sets, estimation of whole-basin and sub-basin carbon and sediment budgets, development and implementation of terrestrial carbon-cycle models, and site-specific field studies of relevant processes. The USGS views this project as a "flagship" effort to demonstrate its capabilities to address the importance of the land surface to biogeochemical problems such as the global carbon budget.

Open-File Report↗

USGS2022

Yucaipa valley integrated hydrological model

Introduction The hydrologic system in the Yucaipa Valley watershed (YVW) was simulated using the coupled Groundwater and Surface-water FLOW model (GSFLOW; Markstrom and others, 2008). This study uses version 2.0 of GSFLOW, which is a combination of the Precipitation-Runoff Modeling System (PRMS; Markstrom and others, 2015), and the Newton-Raphson formulation of the Modular Groundwater-Flow Model (MODFLOW-NWT; hereafter referred to as MODFLOW; Harbaugh, 2005; Niswonger and others, 2011). GSFLOW partitions the hydrologic system into three regions (fig. B1) that are linked by the exchange of unsaturated and saturated groundwater and surface water. The properties and processes within each region influence the flow of both groundwater and surface water into, out of, and within each region. The PRMS component of GSFLOW simulates Region 1, and the MODFLOW component simulates Regions 2 and 3. In the YVW, GSFLOW was applied as the simulation code and is referred to herein as the Yucaipa Integrated Hydrologic Model (YIHM; Alzraiee and others, 2022). In the YIHM, Region 1 includes the plant canopy, snowpack, and the soil zone; Region 2 includes the stream network; and Region 3 includes the subsurface beneath Regions 1 and 2 and consists of both the saturated and unsaturated zones. Soil-moisture conditions and head relations control the flow of both groundwater and surface water between regions. The maximum lateral extents of Regions 1 and 3 were defined using the surface-water drainage divides described in the “Description of Study Area” section of chapter A of this report. The boundaries for Region 2 are the lowest elevation of the streambeds, the stream channel widths, and the horizontal extent of the stream channels in the YVW. Flow across the unsaturated part of Region 3 is assumed to be vertical and does not cross the lateral boundary. To simulate hydrologic processes occurring within the YVW using GSFLOW, a model domain was defined to match the surface watershed such that the domain includes each surficial hydrologic unit coinciding (at least partially) with the Yucaipa groundwater subbasin (hereafter referred to as “Yucaipa subbasin”) as defined in California Bulletin 118 (California Department of Water Resources, 2016). The resulting simulated domain (fig. B2) includes the Yucaipa subbasin and intersects partially with parts of the San Bernardino and San Timoteo groundwater subbasins (fig. B2). The area of the active model domain in YIHM is about 121 square miles (mi2). The developed YIHM can be used to improve understanding of the hydrologic processes in YVW and to simulate future management scenarios with different climatic and anthropogenic changes.

Scientific Investigations Report↗

USGS2012

Effects of prescribed burning on marsh-elevation change and the risk of wetland loss

Marsh-elevation change is the net effect of biophysical processes controlling inputs versus losses of soil volume. In many marshes, accumulation of organic matter is an important contributor to soil volume and vertical land building. In this study, we examined how prescribed burning, a common marsh-management practice, may affect elevation dynamics in the McFaddin National Wildlife Refuge, Texas by altering organic-matter accumulation. Experimental plots were established in a brackish marsh dominated by Spartina patens , a grass found throughout the Gulf of Mexico and Atlantic marshes. Experimental plots were subjected to burning and nutrient-addition treatments and monitored for 3.5 years (April 2005 – November 2008). Half of the plots were burned once in 2006; half of the plots were fertilized seasonally with nitrogen, phosphorus, and potassium. Before and after the burns, seasonal measurements were made of soil physicochemistry, vegetation structure, standing and fallen plant biomass, aboveground and belowground production, decomposition, and accretion and elevation change (measured with Surface Elevation Tables (SET)). Movements in different soil strata (surface, root zone, subroot zone) were evaluated to identify which processes were contributing to elevation change. Because several hurricanes occurred during the study period, we also assessed how these storms affected elevation change rates. The main findings of this study were as follows: 1. The main drivers of elevation change were accretion on the marsh surface and subsurface movement below the root zone, but the relative influence of these processes varied temporally. Prior to Hurricanes Gustav and Ike (September 2008), the main driver was subsurface movement; after the hurricane, both accretion and subsurface movement were important. 2. Prior to Hurricanes Gustav and Ike, rates of elevation gain and accretion above a marker horizon were higher in burned plots compared to nonburned plots, whereas nutrient addition had no detectable influence on elevation dynamics. 3. Burning decreased standing and fallen plant litter, reducing fuel load. Hurricanes Gustav and Ike also removed fallen litter from all plots. 4. Aboveground and belowground production rates varied annually but were unaffected by burning and nutrient treatments. 5. Decomposition (of a standard cellulose material) in upper soil layers was increased in burned plots but was unaffected by nutrient treatments. 6. Soil physicochemistry was unaffected by burning or nutrient treatments. 7. The elevation deficit (difference between rate of submergence and vertical land development) prior to hurricanes was less in burned plots (6.2 millimeters per year [mm yr -1 ]) compared to nonburned plots (7.2 mm yr -1 ). 8. Storm sediments delivered by Hurricane Ike raised elevations an average of 7.4 centimeters (cm), which countered an elevation deficit that had accrued over 11 years. Our findings provide preliminary insights into elevation dynamics occurring in brackish marshes of the Texas Chenier Plain under prescribed fire management. The results of this study indicate that prescribed burning conducted at 3- to 5-year intervals is not likely to negatively impact the long-term sustainability of S. patens -dominated brackish marshes at McFaddin National Wildlife Refuge and may offset existing elevation deficits by ≈ 1 mm yr -1 . The primary drivers of elevation change varied in time and space, leading to a more complex situation in terms of predicting how disturbances may alter elevation trajectories. The potential effect of burning on elevation change in other marshes will depend on several site-specific factors, including geomorphic/ sedimentary setting, tide range, local rate of relative sea level rise, plant species composition, additional management practices (for example, for flood control), and disturbance types and frequency (for example, hurricanes or herbivore grazing). Increasing the scope of inference would require installation of SETs in replicate marshes undergoing different prescribed fire intervals and in different geomorphic settings (with different hurricane frequencies and/or different sedimentary settings). Multiple locations along the Gulf and Atlantic coasts where prescribed fire is used as a management tool could provide the appropriate setting for these installations.

Open-File Report↗

USGS2019

A 3-year in-situ measurement of CO2 efflux in coastal wetlands: Understanding carbon loss through ecosystem respiration and its partitioning

Understanding the link between ecosystem respiration (Reco) and its influential factors is necessary to evaluate the sources of gaseous carbon loss in coastal wetlands. Seablite (Suaeda salsa Pall.) is the main vegetation type pioneering temperate coastal wetlands in northeast China, and is generally an understudied wetland type. To evaluate the influence of environmental factors on Reco, a multi-year in-situ experiment was carried out during the growing seasons of 2012 to 2014. Total CO2 efflux was measured and separated further into soil microbial and belowground root respiration (Rs + r) and plant respiration (Rplant). Reco displayed strong seasonal variation, with effluxes as high as 845 to 1150 mg CO2 m−2 h−1 during summer months and as low as 32 to 111 mg CO2 m−2 h−1 during spring (when new shoots are sprouting) and fall (when plants are senescing) months. Aboveground plant structures contributed on average 79% to total plant biomass, and accounted for most of the Reco measured; i.e., 62–96% was associated as Rplant. Plant activity was strongly seasonal, accordingly driving Reco, with 1 g of soil-emergent S. salsa biomass (dry weight) producing approximately 1.58 mg CO2 per hour toward Reco during mid-summer. When water level was below the soil surface, Rs + r was exponentially correlated to air temperature. Because Reco for S. salsa marsh in the Liaohe Delta is controlled by plant growth cycles, inundation regime, and air temperature, this finding may be applied for national carbon budget estimation purposes from S. salsa wetlands throughout Northeast China and potentially close a key gap in understanding the role of this large wetland area in contributing to respiratory CO2 emissions globally.

Wetlands↗

USGS2019

Evidence for widespread microbivory of endophytic bacteria in roots of vascularplants through oxidative degradation in root cell periplasmic spaces

In this chapter we present a hypothesis, and data supporting it, that vascular plants in diverse families possess symbiotic/endophytic bacteria that frequently vector on or within their seeds; seedlings degrade symbiotic bacteria within roots. Evidence of widespread microbivory was found in a survey for intracellular bacteria that we conducted including seedlings in 36 species of vascular plants distributed in 20 plant families. Experiments indicate that when seeds germinate, bacteria colonize seedlings and internalize into root cells where they are oxidatively-degraded in the periplasmic spaces of cells. The process of degradation of microbes in roots has been termed “rhizophagy”, and “rhizophagy cycle” or “rhizophagy symbiosis” in the case of symbiotic bacteria that alternate between a free-living soil phase and intracellular/endophytic phase. We hypothesize that microbivory could account for a significant portion of nutrients acquired by plants from soils—with one estimate suggesting that as much as 30% of the nutrients acquired by seedlings may stem from rhizophagy symbiosis. Developing a better understanding of rhizophagy symbiosis could lead to new ways to cultivate crops without reliance on excessive agrochemical applications. Learning how to manipulate rhizophagy symbiosis could result in new technologies for reducing growth of weedy or invasive plant species by inhibiting rhizophagy symbiosis.

Book chapter↗

USGS2022

Histochemical evidence for nitrogen‐transfer Endosymbiosis in non‐photosynthetic cells of leaves and inflorescence bracts of angiosperms

We used light and confocal microscopy to visualize bacteria in leaf and bract cells of more than 30 species in 18 families of seed plants. Through histochemical analysis, we detected hormones (including ethylene and nitric oxide), superoxide, and nitrogenous chemicals (including nitric oxide and nitrate) around bacteria within plant cells. Bacteria were observed in epidermal cells, various filamentous and glandular trichomes, and other non-photosynthetic cells. Most notably, bacteria showing nitrate formation based on histochemical staining were present in glandular trichomes of some dicots (e.g., Humulus lupulus and Cannabis sativa ). Glandular trichome chemistry is hypothesized to function to scavenge oxygen around bacteria and reduce oxidative damage to intracellular bacterial cells. Experiments to assess the differential absorption of isotopic nitrogen into plants suggest the assimilation of nitrogen into actively growing tissues of plants, where bacteria are most active and carbohydrates are more available. The leaf and bract cell endosymbiosis types outlined in this paper have not been previously reported and may be important in facilitating plant growth, development, oxidative stress resistance, and nutrient absorption into plants. It is unknown whether leaf and bract cell endosymbioses are significant in increasing the nitrogen content of plants. From the experiments that we conducted, it is impossible to know whether plant trichomes evolved specifically as organs for nitrogen fixation or if, instead, trichomes are structures in which bacteria easily colonize and where some casual nitrogen transfer may occur between bacteria and plant cells. It is likely that the endosymbioses seen in leaves and bracts are less efficient than those of root nodules of legumes in similar plants. However, the presence of endosymbioses that yield nitrate in plants could confer a reduced need for soil nitrogen and constitute increased nitrogen-use efficiency, even if the actual amount of nitrogen transferred to plant cells is small. More research is needed to evaluate the importance of nitrogen transfer within leaf and bract cells of plants

Biology↗