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1,607 records · Page 31Linked to original sources
USGS1996

Technique for estimating magnitude and frequency of peak flows in Maryland

A convenient and reliable technique for estimating flood magnitudes is required for effective flood-plain management and for the efficient design of bridges, culverts, embankments, and flood-protection structures. Methods are presented for estimating peak-flow magnitudes of selected frequencies, ranging from 2 to 500 years, for all nontidal drainage basins in Maryland. The methods were developed by generalized least-squares regression techniques using data from 219 gaged basins in and near Maryland. The State is divided into five hydrologic regions: the Appalachian Plateaus and Allegheny Ridges region, the Blue Ridge and Great Valley region, the Piedmont region, the Western Coastal Plain region, and the Eastern Coastal Plain region. These regions correspond to the physiographic provinces of the State, with the exceptions that (1) the Coastal Plain Province is divided into two hydrologic regions, and (2) there is no distinct hydrologic region corresponding to the Valley and Ridge Province as it is divided into its constituent Allegheny Ridges and Great Valley subdivisions. Sets of equations for calculating peak discharges based on physical basin characteristics are provided for each of the regions. Based on the peak-flow equations, methods for estimating peak flows are presented for ungaged and gaged streams in Maryland. The methods and equations are supported by generalized least-squares analysis of basin and flood-frequency characteristics data from 219 drainage basins in and near Maryland. estimates for each of the five regions are calculated using combinations of the fol-lowing basin characteristics: drainage area, forest cover, basin relief, carbonate rock coverage, storage, and runoff-curve number. Drainage area contributes to the estimate in all five study regions. Carbonate rock coverage is used only in the Blue Ridge and Great Valley region. Storage and runoff-curve number are used solely in the Eastern Coastal Plain region. All other basin characteristics are used in two or more regions. Standard errors of estimate for the regression equations range from 19 to 31 percent in the Appalachian Plateaus and Allegheny Ridges region, 34 to 47 percent in the Blue Ridge and Great Valley region, 33 to 48 percent in the Piedmont region, 45 to 64 percent in the Western Coastal Plain region, and 36 to 42 percent in the Eastern Coastal Plain region.

Maryland↗
USGS2024

Geoelectric evidence for a wide spatial footprint of active extension in central Colorado

Three-dimensional magnetotelluric (MT) imaging in central Colorado reveals a set of north-striking high-conductivity tracks at lower-crustal (50–20 km) depths, with conductive finger-like structures rising off these tracks into the middle crust (20–5 km depth). We interpret these features to represent saline aqueous fluids and partial melt that are products of active extensional tectonomagmatism. These conductors are distributed over a wider region than the narrow corridor along which Rio Grande rift structures are traditionally mapped at the surface, and they consequently demarcate regions of the lower crust where accommodation of bulk extensional strain has concentrated conductive phases. Our observations reveal limitations in existing models of Rio Grande rift activity and may reflect unrecognized spatiotemporal variations in rift system evolution globally.

Colorado↗
USGS2025

Hydrologic variability and groundwater age of springs in eastern Oregon and northern Nevada, USA

The ecological importance of springs in semiarid regions is far greater than their small size and sparse distribution, yet little is known about the hydrologic functioning of these systems. During 2016–22, 261 springs were visited in the volcanic terrane of eastern Oregon and northern Nevada. When conditions were suitable, measurements of discharge, water temperature, and specific conductance were made, and samples for the analysis of carbon-14, tritium, and water stable isotopes (WSI) were collected. A subset of 60 springs was revisited during different seasons in the same year and during the dry season in multiple years to evaluate variability in discharge, chemistry, and groundwater age. Specific conductance and WSI varied considerably among springs across the study area but were unexpectedly stable across seasons and years at individual springs. Seasonal and interannual variability in spring discharge was related to the residence time of the discharging groundwater. Springs discharging older groundwater (10 3 –10 4 years) had significantly less variability in their discharge compared to springs discharging younger groundwater (10 0 –10 1 years). Variability among springs discharging younger groundwater included cessation of late-summer discharge at 18 % of the repeat-visit springs. A logistic regression model predicted the age of discharging spring water with 89 % accuracy using only the spring latitude, longitude, elevation, and δ 2 H value. This study framework provides a simple, inexpensive, and robust method to provisionally assess the hydrologic behavior of springs having little or no prior information in understudied, semiarid regions across the globe.

Nevada, Oregon↗
USGS2000

Quality assurance and analysis of water levels in wells on Pahute Mesa and vicinity, Nevada Test Site, Nye County, Nevada

Periodic and continual water-level data from 1963 to 1998 were compiled and quality assured for 65 observation wells on Pahute Mesa and vicinity, Nye County, Nevada. As part of the quality assurance of all water levels, ancillary data pertinent to computing hydraulic heads in wells were compiled and analyzed. Quality-assured water levels that were not necessarily in error but which did not represent static heads in the regional aquifer system, or required some other qualification, were flagged. Water levels flagged include those recovering from recent pumping or well construction, water levels affected by nuclear tests, and measurements affected by borehole deviations. A cursory examination of about 30 wells with available water-level and down-hole temperature data indicate that water levels in most wells on Pahute Mesa would not be significantly affected by temperature if corrected to 95 degrees Fahrenheit. Wells with large corrections (greater than 10 feet) are those with long water columns (greater than 1,500 feet of water above the assumed point of inflow) in combination with mean water-column temperatures exceeding 105 degrees Fahrenheit. Water-level fluctuations in wells on Pahute Mesa are caused by several factors including infiltration of precipitation, barometric pressure, Earth tides, ground-water pumpage, and seismic events caused by tectonic activity and underground nuclear testing. No observed water-level fluctuations were attributed to a naturally occurring earthquake. The magnitude and duration of changes in water levels caused by nuclear tests are affected by the test size and the distance from a well to the test. Identifying water levels that might be affected by past nuclear tests is difficult because pre-testing water-level data are sparse. Hydrologically significant trends were found in 13 of 25 wells with multiple years of water-level record. The largest change in water levels (1,029 feet in 25 years) occurred in well U-19v PS 1D as a result of the Almendro nuclear test. Likely explanations for trends in most of the wells are either changes in precipitation patterns that affect recharge rates to the ground-water system, pumping effects from water-supply well U-20 WW, or a combination of these two factors.

Nevada↗
USGS2026

Ice sheet dynamics drive pronounced changes in the subsurface freshwater-saltwater interface

Saltwater is migrating into freshwater aquifers globally with water quality and biogeochemical implications, yet saltwater intrusion in glaciated regions is sparsely investigated. Field observations suggest that groundwater head in glaciated systems is influenced by ice sheet forcings and provides evidence that seawater infiltrated into offshore aquifers during past deglaciation events. To understand links between ice sheet dynamics, groundwater head, and saltwater intrusion, we use numerical models to explore the effects of deglaciation on nearshore head and salinity distributions. We find that ice sheet thinning diminishes groundwater head, and the resulting shift in subsurface pressure gradients drives rapid landward movement of the subsurface freshwater-saltwater interface up to 4.0 km or 1.3 m per m ice sheet loss. Results highlight an overlooked saltwater intrusion mechanism that aligns with field observations and affects glaciated coastlines undergoing ice sheet retreat, underscoring the need to consider this mechanism in studies of contemporary coastal water quality.

Geophysical Research Letters↗
USGS2024

On connecting hydro-social parameters to vegetation greenness differences in an evolving groundwater-dependent ecosystem

Understanding groundwater-dependent ecosystems (i.e., areas with a relatively shallow water table that plays a major role in supporting vegetation health) is key to sustaining water resources in the western United States. Groundwater-dependent ecosystems (GDEs) in Colorado have non-pristine temporal and spatial patterns, compared to agro-ecosystems, which make it difficult to quantify how these ecosystems are impacted by changes in water availability. The goal of this study is to examine how key hydrosocial parameters perturb GDE water use in time and in space. The temporal approach tests for the additive impacts of precipitation, surface water discharge, surface water mass balance as a surrogate for surface–groundwater exchange, and groundwater depth on the monthly Landsat normalized difference vegetation index (NDVI). The spatial approach tests for the additive impacts of river confluences, canal augmentation, development, perennial tributary confluences, and farmland modification on temporally integrated NDVI. Model results show a temporal trend (monthly, 1984–2019) is identifiable along segments of the Arkansas River at resolutions finer than 10 km. The temporal impacts of river discharge correlate with riparian water use sooner in time compared to precipitation, but this result is spatially variable and dependent on the covariates tested. Spatially, areal segments of the Arkansas River that have confluences with perennial streams have increased cumulative vegetation density. Quantifying temporal and spatial dependencies between the sources and effects of GDEs could aid in preventing the loss of a vulnerable ecosystem to increased water demand, changing climate, and evolving irrigation methodologies.

Colorado↗
USGS1992

Description and effects of 1988 drought on ground-water levels, streamflow, and reservoir levels in Indiana

Documentation of the 1988 drought in Indiana was undertaken to aid water-management agencies and planners concerned with periods of below-normal precipitation and their effect on commercial, agricultural, and residential water use. Precipitation, temperature, Palmer Drought Severity Indices, and ground- and surface-water levels from water years 1988 and 1989 were compared to the historical record to evaluate severity, extent, and duration of the 1988 drought in Indiana. Three types of drought-climatological, hydrologic, and agricultural--occurred in most of Indiana during water years 1988 and 1989. The drought began toward the end of calendar year 1987 as annual precipitation decreased to 4.6 inches below the long term mean. By the end of September 1988, statewide precipitation deficits had increased to almost 8 inches below normal. High temperatures during the summer months increased the stress on crops, livestock, and people. Northwest Indiana experienced the second warmest June-August on record. Palmer Drought Severity Indices indicated that a moderate-to-severe drought had occurred in Indiana during most of 1988. Ground-water levels were affected substantially in many areas of the State. Record low-water levels were observed at 12 of the 20 monitoring wells included in this report. A go-day ground-water emergency was declared in parts of northwestern Indiana. Streamflow throughout the State was affected to varying degrees by the drought. Annual mean discharge in some rivers was only slightly less than the mean annual discharge, while others flowed at less than half that value. The effects of low streamflows were felt by many as electric power plants reduced or ceased production and public-water utilities requested conservation measures by their customers. Major reservoirs in the State approached or reached record low levels, causing water supplies as well as recreational activities to be diminished. Most major crops produced in Indiana were affected by the dry conditions. Average yields in 1988 ranged from 50 to 86 percent of 1987 yields.

Indiana↗
USGS2023

WellSTIC: A cost-effective sensor for performing point dilution tests to measure groundwater velocity in shallow aquifers

Many individual measurement points are required to characterize groundwater velocity within an aquifer. Groundwater velocity is most commonly measured using a network of >5 cm diameter monitoring wells, which, if not already present at a site, are expensive and labor-intensive to install. Drive-point piezometers—simple, cost-effective wells that can be installed by hand—are a common tool for sampling groundwater in shallow, alluvial aquifers, but most groundwater velocity measurement techniques require equipment that is too large for these narrow (usually <2 cm inside diameter) piezometers. In this technical note, we introduce a low-cost sensor and well packer system (<$90 USD) for performing point dilution tests in narrow piezometers. Field data show that the magnitude of groundwater velocity measured with this technique agrees with velocities computed from natural gradient tracer tests. Additionally, with proper calibration, these sensors can be used to continuously monitor in-well specific conductance, either during inter-well tracer tests with saline tracers or for water quality monitoring. This system is a viable tool for rapid assessment of the magnitude of groundwater velocity in shallow aquifers.

Water Resources Research↗
USGS2024

Chloride concentrations in groundwater from the western part of the Southern Hills regional aquifer system, Louisiana, 2021–22

Groundwater is heavily used for public supply and industrial uses in the Baton Rouge, Louisiana, area. Lowered water levels resulting from groundwater withdrawals have induced the movement of saltwater towards wells in East Baton Rouge and West Baton Rouge Parishes. Saltwater intrusion has the potential to affect water supply infrastructure, reduce water availability for some uses, and increase treatment costs. To document current conditions, samples were collected from 161 wells screened in 10 aquifers of the Southern Hills regional aquifer system during November 2021 through February 2022. The results were compared with historical data to identify where chloride concentrations are increasing, which could indicate that saltwater intrusion is occurring. Saltwater intrusion, to varying degrees and areal extents, was observed in most of the 10 aquifers. The limited availability of monitoring wells near or within some of the known saltwater plume areas restricts tracking of the movement or delineation of the plumes’ current extents.

Louisiana↗
USGS2026

Microtextural characteristics of adularia in banded quartz veins from the Midas low-sulfidation epithermal deposit, Nevada

High-grade ores at the Miocene Midas low-sulfidation epithermal deposit in northern Nevada are confined to crustiform quartz veins containing abundant adularia. Micro-X-ray fluorescence elemental mapping reveals that adularia is a common gangue mineral occurring in colloform bands, bands showing bladed textures, and bands with dendritic terminations. The adularia aggregates have delicate shapes and are comprised of stacked, submillimeter crystals hosted by fine-grained quartz. The textural evidence suggests that the adularia aggregates originally formed within a gel-like, noncrystalline silica matrix, which subsequently transformed into quartz. This indicates that the adularia did not precipitate in open space along the vein walls. Correlative microscopy, involving scanning electron microscopy-based automated mineralogy and optical petrography, demonstrates that bands containing abundant adularia are not the primary host to ore minerals. The ore minerals occur in different bands within the crustiform veins, implying that adularia and ore mineral precipitation did not always occur simultaneously. It is hypothesized here that fluid flow at Midas involved intermittent short-lived events of fluid flashing, causing rapid solute supersaturation in the liquid. During each flashing event, different amounts of vapor were produced along a given vein. Compositional differences between adjacent bands in the crustiform quartz veins may, therefore, be linked to variations in the amount of vapor formed during each flash event.

Nevada↗
USGS2025

Aftershocks in stress shadows are inconsistent with modeled static Coulomb stress changes

Aftershock triggering is commonly attributed to increases in static Coulomb stress. In some areas, termed "stress shadows", a decrease in Coulomb stress is predicted to suppress earthquake occurrence. However, aftershocks are often observed in the modeled stress shadows. We examine several hypotheses that attempt to reconcile these shadow aftershocks with the static Coulomb stress change model: (1) they appear to be in shadows because of inaccuracy in the stress change calculations, (2) they occur on faults of unusual orientation which actually experienced increased Coulomb stress, (3) they occur on faults with different frictional properties, not modeled well by Coulomb stress, and (4) they are secondary aftershocks triggered by prior aftershocks or afterslip. When tested on the 2016 Mw7.0 Kumamoto, Japan, and 2019 Mw7.1 Ridgecrest, California, aftershock sequences, none of these hypotheses can explain the majority of the shadow aftershocks, and taken together these hypotheses can explain only about half of these aftershocks. This implies that Coulomb stress modeling that lacks small-scale fault zone heterogeneity might be inadequate to fully capture the true static stress changes and/or that other physical triggering models are needed, for example transient processes such as delayed triggering by dynamic stress changes from the passing seismic waves.

California↗
USGS2024

Recent advances in characterizing the crustal stress field and future applications of stress data: Perspectives from North America

The stress field controls patterns of crustal deformation, including which faults are likeliest to cause earthquakes or transmit fluids. Since the 1950s, maps of maximum horizontal stress ( S Hmax ) orientations have advanced dramatically, and the style of faulting (relative principal stress magnitudes) has recently been mapped in some regions as well. This perspectives paper summarizes developments in characterizing stress orientations and (relative) magnitudes, including new seismic and borehole methods, as well as progress in identifying the causes of stress variations. Despite these advances, adding far more spatiotemporal detail would allow geoscientists to address many of today's key challenges regarding natural hazards, energy development, and geodynamics. In particular, it is critically important to characterize stress heterogeneity at multiple scales while also recognizing the coherent variability of the stress field. The second part of the paper considers how more detailed stress datasets could prove essential to addressing some of the grand questions in geoscience, including deciphering the poorly understood feedbacks between crustal dynamics and surface processes, improving earthquake and eruption forecasts, and determining the origins and shared properties of plate boundaries.

Geological Society, London, Special Publications↗
USGS2025

Airborne radiometric data map alteration of porphyry copper systems in the Elkhorn district, MT

The Elkhorn district in southwestern Montana is known for past production of high-grade base and precious metals from skarn and carbonate replacement deposits. The district also hosts a porphyry Cu-Mo deposit. A radiometric survey flown over the district and surrounds provides maps of surficial concentrations of potassium, thorium, and uranium. Handheld gamma ray spectrometer measurements on altered and unaltered rocks in the district and surrounding region provide a means to ground truth the airborne data. We demonstrate that the airborne and ground-based measurements show similar map patterns. We calculate ratios of the radioelement concentrations and present a potassium enhancement map that combines potassium and ratios of potassium to thorium and uranium. The results highlight the rocks previously mapped as having K-feldspar-biotite and quartz-sericite alteration assemblages in the Elkhorn district and map additional hydrothermal systems in the region, including known alteration in the Radersburg district. The data enhancement techniques can be used as a screening tool for mapping additional porphyry copper systems.

Montana↗
USGS2025

The role of bedrock circulation depth and porosity in mountain streamflow response to prolonged drought

Quantitative understanding is lacking on how the depth of active groundwater circulation in bedrock affects mountain streamflow response to a multi‐year drought. We use an integrated hydrological model to explore the sensitivity of a variety of streamflow metrics to bedrock circulation depth and porosity under a plausible extreme drought scenario lasting up to 5 years. Endmember depth versus hydraulic conductivity relationships and porosity values for fractured crystalline rock are simulated. With drought, a deeper circulation system with higher drainable porosity more effectively buffers minimum flow and significantly limits perennial stream loss in comparison to a shallow circulation system. Streamflow buffering is accomplished through extensive groundwater storage loss. However, deeper circulation systems experience prolonged recovery from drought in comparison to storage‐limited shallow systems. Research highlights the importance of characterizing the deeper bedrock hydrogeology in mountainous watersheds to better understand and predict drought impacts on stream ecosystem health and water resource sustainability.

Colorado↗
USGS2025

A soil velocity model for improved ground motion simulations in the U. S. Pacific Northwest

Near-surface seismic velocity structure may significantly impact the intensity, duration, and frequency content of ground shaking during an earthquake. In this study, we compile 649 shear wave velocity (Vs) profiles throughout the U.S. Pacific Northwest and southern British Columbia (PNW) and use these measured profiles to develop a representative soil velocity model for four major Holocene soil provinces: Puget Lowlands, Willamette Valley, fill and alluvium, and `other' soils. The resulting soil velocity model shows good agreement to measured data for a wide range of site conditions, with variability between different geologic domains reflecting fundamental differences in depositional environments. We then show that using this regional soil velocity model in simulations of the 2001 M6.8 Nisqually, Washington earthquake improves the fit to observed high-frequency (≥ 0.5 Hz) ground motions in the Puget Sound region compared to simulations that do not incorporate shallow (≤ 200 m) seismic velocity structure. Overall, this work shows that incorporating localized soil velocity profiles into seismic velocity models is important for accurately estimating high-frequency ground motion and regional seismic hazard in earthquake simulations. Future earthquake simulations and hazard studies in the PNW could incorporate these soil velocity profiles to capture the region's distinct site response characteristics.

Washington↗
USGS1999

Concentrations and distribution of manmade organic compounds in the Lake Tahoe Basin, Nevada and California, 1997-99

The U.S. Geological Survey, in cooperation with the Tahoe Regional Planning Agency and the Lahontan Regional Water-Quality Control Board, sampled Lake Tahoe, major tributary streams to Lake Tahoe, and several other lakes in the Lake Tahoe Basin for manmade organic compounds during 1997-99. Gasoline components were found in all samples collected from Lake Tahoe during the summer boating season. Methyl tert -butyl ether (MTBE), benzene, toluene, ethylbenzene, and xylenes (BTEX) were the commonly detected compounds in these samples. Most samples from tributary streams and lakes with no motorized boating had no detectable concentrations of gasoline components. Motorized boating activity appears to be directly linked in space and time to the occurrence of these gasoline components. Other sources of gasoline components to Lake Tahoe, such as the atmosphere, surface runoff, and subsurface flow, are minor compared to the input by motorized boating. Water sampled from Lake Tahoe during mid-winter, when motorized boating activity is low, had no MTBE and only one sample had any detectable BTEX compounds. Soluble pesticides rarely were detected in water samples from the Lake Tahoe Basin. The only detectable concentrations of these compounds were in samples from Blackwood and Taylor Creeks collected during spring runoff. Concentrations found in these samples were low, in the 1 to 4 nanograms per liter range. Organochlorine compounds were detected in samples collected from semipermeable membrane devices (SPMD's) collected from Lake Tahoe, tributary streams, and Upper Angora Lake. In Lake Tahoe, SPMD samples collected offshore from urbanized areas contained the largest number and highest concentrations of organochlorine compounds. The most commonly detected organochlorine compounds were cis- and trans-chlordane, p , p' -DDE, and hexachlorobenzene. In tributary streams, SPMD samples collected during spring runoff generally had higher combined concentrations of organochlorine compounds than those collected during baseflow conditions. Upper Angora Lake had the fewest number of organochlorine compounds detected of all lake samples. Dioxins and furans were not detected in SPMD samples from two sites in Lake Tahoe or from two tributary streams. The number of polycyclic aromatic hydrocarbon (PAH) compounds and their combined concentrations generally were higher in samples from Lake Tahoe than those from tributary streams. Areas of high-motorized boating activity at Lake Tahoe had the largest number and highest concentrations of PAH's. PAH compounds were detected in samples from SPMD's in four of six tributary streams during spring runoff, all tributary streams during baseflow conditions, and at all lake sites. The most commonly detected PAH's in tributary streams during spring runoff were phenanthrene, fluoranthene, pyrene, and chrysene, and during baseflow conditions were phenanthrene, 1-methylphenanthrene, diethylnaphthalene, and pyrene. Upper Truckee River, which has an urban area in its drainage basin, had the largest number and highest combined concentration of PAH's of all stream samples. Bottom-sediment from Lake Tahoe had detectable concentrations of p-cresol, a phenol, in all but one sample. A sample collected near Chambers Lodge contained phenol at an estimated concentration of 4 micrograms per kilogram (µg/kg). Bottom-sediment samples from tributary streams had no detectable concentrations of organochlorine or PAH compounds. Several compounds were detected in bottom sediment from Upper Angora Lake at high concentrations. These compounds and their concentrations were p , p' -DDD (10 µg/kg), p , p' -DDE (7.4 µg/kg), 2,6-dimethylnaphthalene (estimated at 190 µg/kg), pentachlorophenol (3,000 µg/kg), and p-cresol (4,400 µg/kg).

California, Nevada↗
USGS2024

Land-use interactions, Oil-Field infrastructure, and natural processes control hydrocarbon and arsenic concentrations in groundwater, Poso Creek Oil Field, California, USA

Like many hydrocarbon production areas in the U.S., the Poso Creek Oil Field in California includes and is adjacent to other land uses (agricultural and other developed lands) that affect the hydrology and geochemistry of the aquifer overlying and adjacent to oil development. We hypothesize that the distributions of hydrocarbons and arsenic in groundwater in such areas will be controlled by complex interactions between mixed land uses, oil-field infrastructure, and natural processes. In 2020–2021, samples of groundwater and surface water were collected and analyzed for a large suite of inorganic and organic chemicals and isotope and gas tracers to test this hypothesis. Those data are supplemented with ancillary data on historical geochemistry, hydrology, geology, and oil-field infrastructure. Hydrocarbons in groundwater (e.g., methane through pentane gases and benzene) are associated with natural processes (e.g., fault offsets or transition in sediment depositional environment) and oil-field infrastructure (e.g., fluid-migration pathways associated with uncemented annulus in oil wells or unlined pits). Arsenic concentrations >10 μg per liter (μg/L; maximum concentration 12.9 μg/L) are associated with natural processes in old, high-pH groundwater, and more recent recharge of water from natural and/or engineered recharge processes. Along the southwest margin of the oil field, pumping for drinking-water and irrigation supplies in combination with engineered groundwater recharge produce a depression in groundwater elevations where groundwater with elevated sulfate concentrations from agricultural areas and groundwater with hydrocarbons from the oil field mix to produce a zone of sulfate reduction that removes hydrocarbons and arsenic from groundwater but produces elevated sulfide (S 2- ) concentrations (maximum concentration 29 mg per liter, mg/L). In this study, multiple approaches were required to resolve the overlapping effects of land uses, oil-field infrastructure, and natural processes on the distributions of hydrocarbons and arsenic in groundwater. The combined use of geographic, historical, physical, chemical, isotopic, and other information to constrain processes could be a useful approach for studies in other hydrocarbon-production areas. This is particularly important where land uses affect aquifer hydrology to an extent that causes mixing of groundwaters with different chemical compositions.

California↗
USGS2025

Detection of viral, bacterial, and protozoan pathogens and microbial source tracking markers in paired large- and small-volume water samples

When sampling for waterborne microbes, researchers may need to diverge from recommended sample volumes due to logistical constraints, novel targets, or challenging matrices, with little guidance about the potential impact on results. In field studies, we measured bacteria, viruses, and protozoa (15 quantitative polymerase chain reaction assays) in paired large- and small-volume samples to evaluate method performance and relevant factors. Concordance between methods was low. Large-volume ultrafiltration yielded more detections than small-volume sampling, especially for pathogens in groundwater. Greater microbial concentrations were associated with more frequent detections in small-volume samples and greater concordance between paired samples. Large-volume samples appeared to be more susceptible to diminished sensitivity from complex sample matrices. In laboratory studies, recovery of microbes was poorer for large- than small-volume methods, although large-volume methods more reliably detected low-concentration targets. Large-volume samples were less stable than small-volume samples during storage. Overall, large-volume sampling was superior for detecting pathogens but may underestimate concentrations; small-volume sampling was more prone to false negatives but was adequate when concentrations were relatively high, like we observed for microbial source tracking in surface waters.

Wisconsin↗