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Colorado Delta riparian plant health improvement

The riparian corridor along Mexico’s arid Colorado River Delta is being affected by reduction in river flow and increases in heat, drought, human infrastructure, and disturbances. These disturbances can change riparian land cover by limiting water availability for riparian plant species, increasing fire intensity and frequency, and increasing soil and water salinities. In response to these forms of degradation, restoration efforts have begun to restore riparian habitats and native plant health, but vegetation greenness and corresponding plant water use continue to decline in unrestored reaches. Researchers from the U.S. Geological Survey (USGS) Southwest Biological Science Center are monitoring riparian plant health along the Colorado River Delta to support better ecohydrological decision-making. The researchers are helping a binational team to protect, restore, and maintain native vegetation within the 150-km long riparian corridor. Researchers are using Landsat 8 Operational Land Imager (OLI) data spanning 2014–2022 to measure greenness, a proxy for plant health, and actual evapotranspiration (ETa). Using an empirical model for ETa, evapotranspiration is estimated over each 16-day Landsat 8 OLI overpass period by considering the 8 days before and after the overpass date. In their paper, researchers noted an increase in vegetation greenness within the restoration sites over nine years, with an average increase of 41.3%, which may be partially due to targeted water deliveries at the restoration sites. Conversely, greenness in adjacent, unrestored control areas declined by 27.3%. The study showed a 22.1% increase in ETa in restored areas, compared to a 30.8% reduction in unrestored regions. Restored sites in one restored area experienced ETa increases up to 12.2%, whereas their unrestored counterparts showed a decline of 21.4%. These estimates of riparian greenness and water use may assist natural resource managers who are tasked with allocating water and managing habitats within similar riparian corridors.

Conference Paper

Evaluating the relationship among wetland vertical development, elevation capital, sea-level rise and tidal marsh sustainability

Accelerating sea-level rise and human impacts to the coast (e.g., altered sediment supply and hydrology, nutrient loading) influence the accumulation of sediment and organic matter, and thereby impact the ability of coastal tidal wetlands to maintain an elevation consistently within the vegetation growth range. Critical components of marsh sustainability are the marsh elevation within the vegetation growth range (elevation capital) and the rates of marsh surface elevation change and relative sea-level rise. The relationship among these factors and their combined influence on marsh integrity were evaluated by comparing trends in surface elevation change on five salt marsh sites located on three marsh islands in Jamaica Bay, NY, USA. All marsh sites were located in a similar physical setting (i.e., tidal range, sea-level rise rate, sediment supply). The structural integrity of the marshes ranged from densely vegetated (high integrity) to severely deteriorated (low integrity) with elevation capital ranging from high to low, respectively, and included a deteriorating marsh site that was partially restored. Two marshes with high elevation capital maintained their relative position high within the tidal range through a combination of surface sediment deposition and shallow subsurface expansion, and kept pace with local sea-level rise. A marsh with moderate elevation capital showed signs of flooding stress and was deteriorating, but managed to keep pace with local sea-level rise. The deteriorated marsh gained no elevation over the 14-year study and was located too low within the tidal range to support continuous coverage of salt marsh vegetation. Elevation gain in the restored marsh initially lagged behind sea-level rise for 8 years, but the elevation trend recovered and kept pace with sea-level rise for the last 5 years. A conceptual model is presented that describes the relationship among elevation capital, and rates of marsh elevation gain and sea-level rise. Note that a search for factors influencing wetland loss should focus on process changes to marsh vertical development (e.g., sediment supply, vegetation growth) and climate change effects (e.g., sea-level and temperature rise) that can cause elevation gain to lag behind sea-level rise, and these occur prior to the onset of marsh deterioration.

New York

Annotated bibliography of scientific research on Ventenata dubia published from 2010 to 2020

Integrating recent science into management decisions supports effective natural resource management and can lead to better resource outcomes. However, finding and accessing science information can be time consuming and costly. To assist in this process, the U.S. Geological Survey (USGS) is creating a series of annotated bibliographies on topics of management concern for western lands. Previously published reports introduced a methodology for preparing annotated bibliographies to facilitate the integration of recent, peer-reviewed science into resource management decisions. Therefore, relevant text from those efforts is reproduced here to frame the presentation. Invasive annual grasses are widely distributed throughout the western United States and threaten native ecosystems by altering fire regimes, replacing native plants, and altering grazing patterns, often with tremendous associated costs. One invasive annual grass, Ventenata dubia (hereafter, ventenata), was first introduced to the United States in the 1950s and has recently been identified as a management concern. Ventenata has a wide native geographic range, from Africa to northern Europe, and could thus potentially spread widely in the United States if left unmanaged. We compiled and summarized peer-reviewed journal articles, government reports, and data products on ventenata published between January 1, 2010, and August 27, 2020. We first conducted a systematic search of three reference databases and three government databases using the search phrase: “ventenata” OR “Ventenata dubia.” We refined the initial list of products by removing (1) duplicates, (2) products not written in English, (3) publications that were not focused in North America, (4) publications that were not published as research, data products, or scientific review articles in peer-reviewed journals or as formal technical reports, and (5) products for which ventenata was not a research focus or for which the study did not present new data or findings about ventenata. We summarized each product using a consistent structure (background, objectives, methods, location, findings, and implications) and identified the management topics (for example, species and population characteristics, habitat, control and management efforts) addressed by each product. We also noted which publications included new geospatial data. The review process for this annotated bibliography included an initial internal colleague review of each summary, requesting input on each summary from an author of the original publication, and a formal peer review. Our initial searches resulted in 505 total products, of which 29 met our criteria for inclusion. Nonnative invasive plants; weed management; behavior or demographics; dispersal, spread, vectors and pathways; site-scale habitat characteristics; survival; and weed management subtopic: herbicides were the management topics most commonly addressed. The online version of this annotated bibliography will be searchable by topic, location, and year; it will also include links to each original publication, where available. The studies compiled and summarized here may inform planning and management actions that seek to maintain and restore landscapes and control nonnative invasive species across the western United States.

Open-File Report

Annotated bibliography of scientific research on pygmy rabbits published from 1990 to 2020

Integrating recent scientific knowledge into management decisions supports effective natural resource management and can lead to better resource outcomes. However, finding and accessing scientific knowledge can be time consuming and costly. To assist in this process, the U.S. Geological Survey (USGS) is creating a series of annotated bibliographies on topics of management concern for western lands. Previously published reports introduced a methodology for preparing annotated bibliographies to facilitate the integration of recent, peer-reviewed science into resource management decisions. Therefore, relevant text from those efforts is reproduced here to frame the presentation. Sagebrush ecosystems throughout North America face management challenges including habitat loss and fragmentation. Brachylagus idahoensis (pygmy rabbits) are a sagebrush-obligate species that has experienced population declines and range contraction in recent decades. A disjunct population of pygmy rabbits in the Columbia Basin in Washington was listed as federally endangered in 2003. Due to their specialized habitat requirements and low dispersal ability, pygmy rabbits are a high priority for managers throughout their range. We compiled and summarized peer-reviewed journal articles, data products, and formal technical reports (such as U.S. Forest Service General Technical Reports and U.S. Geological Survey Open-File Reports) on pygmy rabbits published between January 1, 1990 and December 31, 2020. We first conducted a structured search of three reference databases and three government databases using the phrase “pygmy rabbit” or “ Brachylagus idahoensis .” We refined the initial list of products by removing (1) duplicates, (2) products not written in English, (3) publications that were not focused on North America, (4) publications that were not published as research, data products, or scientific review articles in peer-reviewed journals or as formal technical reports, and (5) products for which pygmy rabbits were not a research focus or for which the study did not present new data or findings about pygmy rabbits. We summarized each product using a consistent structure (background, objectives, methods, location, findings, and implications) and identified the management topics (for example, captive breeding, habitat characteristics, and population estimates) addressed by each product. We also noted which publications included new publicly available geospatial data. The review process for this annotated bibliography included an initial internal colleague review of each summary, requesting input on each summary from an author of the original publication, and a formal peer review. Our initial searches resulted in 2,285 total products, of which 105 met our criteria for inclusion. Sensitive/rare wildlife, behavior or demographics, site-scale habitat characteristics, habitat selection, and effects distances or spatial scale were the management topics most commonly addressed. The online version of this bibliography, Science for Resource Managers, will be searchable by topic, location, and year; it will include links to each original publication, where available. The studies compiled and summarized here may inform planning and management actions that seek to maintain and restore sagebrush landscapes and associated native species across the western United States.

Open-File Report

Prioritizing restoration areas to conserve multiple sagebrush-associated wildlife species

Strategic restoration of altered habitat is one method for addressing worldwide biodiversity declines. Within the sagebrush steppe of western North America, habitat degradation has been linked to declines in many species, making restoration a priority for managers; however, limited funding, spatiotemporal variation in restoration success, and the need to manage for diverse wildlife species make decision-making regarding restoration actions challenging. To address the challenge of spatial conservation prioritization, we developed the Prioritizing Restoration of Sagebrush Ecosystems Tool (PReSET). This decision support tool utilizes the prioritizr package in program R and an integer linear programming algorithm to select parcels representing both high biodiversity value and high probability of restoration success. We tested PReSET on a sagebrush steppe system within southwestern Wyoming using distributional data for six species with diverse life histories and a spatial layer of predicted sagebrush recovery times to identify restoration targets at both broad and local scales. While the broad-scale portion of our tool outputs can inform policy, the local-scale results can be applied directly to on-the-ground restoration. We identified restoration priority areas with greater precision than existing spatial prioritizations and incorporated range differences among species. We noted tradeoffs, including that restoring for habitat connectivity may require restoration actions in areas with lower probability of success. Future applications of PReSET will draw from emerging datasets, including spatially-varying economic costs of restoration, animal movement data, and additional species, to further improve our ability to target effective sagebrush restoration.

Wyoming

Annotated bibliography of scientific research on Gunnison sage-grouse published from January 2005 to September 2022

Integrating recent scientific knowledge into management decisions supports effective natural resource management and can lead to better resource outcomes. However, finding and accessing scientific knowledge can be time consuming and costly. To assist in this process, the U.S. Geological Survey is creating a series of annotated bibliographies on topics of management concern for western lands. Previously published reports introduced a methodology for preparing annotated bibliographies to facilitate the integration of recent, peer-reviewed science into resource management decisions. Therefore, relevant text from those efforts is reproduced here to frame the presentation. Centrocercus minimus (Gunnison sage-grouse; hereafter GUSG) has been a focus of scientific investigation since the early 2000s. The U.S. Fish and Wildlife Service listed GUSG as threatened under the Endangered Species Act in 2014 because of declining populations and increasing habitat loss. The U.S. Fish and Wildlife Service, Bureau of Land Management, and Colorado Parks and Wildlife have sought to increase the conservation of this species by adapting management and recovery plans to reduce threats and increase population resiliency. GUSG are studied less than the closely related Centrocercus urophasianus (greater sage-grouse); however, research efforts have recently increased to understand the life history, genetics, and habitat suitability of this sagebrush-obligate species. We compiled and summarized peer-reviewed journal articles, data products, and formal technical reports (such as U.S. Department of Agriculture Forest Service General Technical Reports and U.S. Geological Survey Open-File Reports) on GUSG, published between January 2005 and September 2022. We first systematically searched three reference databases and three government databases using the following search phrases: “Gunnison sage-grouse” or “lesser sage-grouse” or “Gunnison grouse” or “Gunnison sage grouse” or “lesser sage grouse” or “ Centrocercus minimus .” We refined the initial list of products by removing (1) duplicates, (2) publications that were not published as research, data products, or scientific review articles in peer-reviewed journals or as formal technical reports, and (3) products that did not have GUSG as a research focus or products that did not present new data or findings about GUSG. We summarized each product using a consistent structure (background, objectives, methods, location, findings, and implications) and identified the management topics (for example, population estimates or targets, habitat, and management efforts) addressed by each product. We also noted which publications included new geospatial data. The review process for this annotated bibliography included two initial internal colleague reviews of each summary, requesting input on each summary from an author of the original publication, and a formal peer review. Our initial searches resulted in 80 total products, of which 63 met our criteria for inclusion of which 53 were products that had not been summarized before. Across products summarized in the annotated bibliography, broad-scale habitat characteristics; population estimates or targets; behavior or demographics; and genetics were the most commonly addressed management topics. The bibliographies are available on the Science for Resource Managers tool ( https://apps.usgs.gov/science-for-resource-managers ) and are searchable by topic, location, and year, and the search tool includes links to each original publication. The studies compiled and summarized in this annotated bibliography may inform planning and management actions that seek to maintain and restore sagebrush landscapes and GUSG populations across the GUSG range.

Colorado

Impacts of grade control structure installations on hydrology and sediment transport as an adaptive management strategy

The goal of this research was to examine the impacts of Grade Control Structure (GCS) installations at the Heard Scout Pueblo (HSP) study site in the City of Phoenix, Arizona, USA. The study site is around a high-use trail system and is comprised of eroded and incised channels that conduct high flows and associated sediments into a residential neighborhood downstream, a noted stormwater control problem. We established baseline conditions associated with rainfall/runoff response before structures were installed so we could have some data for comparison afterwards. Innovative monitoring equipment, including video cameras and pressure transducers (to calculate discharge); digital terrain models, sediment samplers and sediment chains (to measure erosion and deposition); soil moisture sensors in monitoring wells (to document infiltration and potential recharge); and weather stations (to track temperature and relative humidity) were established and a small Unmanned Aircraft System (sUAS) survey was completed by July, 11, 2017, in time for the typical summer monsoon season which officially runs from June 15th to September 30th. Only one pre-GCS installation rain event incurred a significant flow event (October 13, 2018). Natural Channel Design (NCD), a landscape restoration company with decades of experience, was hired through a competitive bid process to develop a novel layout of ~30 GCS installations (sills, modified one-rock dams (ORD), and plugs, as well as a modified Zuni-bowl). The American Conservation Experience (ACE) hand-built the structures based on these designs in the main channel from November 13, 2018 through December 1, 2018. ACE built another ten structures in locations adjacent to the channel from January 15 through January 18, 2019. NCD worked with the landscape forensics to identify a historic channel and reinstate it using GCS. A surface-water model was also applied, using some of the baseline measurements (terrain and hydraulic conductivity) to track the flows of water and potential infiltration associated with rainfall events before GCS installation, to assist NCD in their design. The same model was applied using the installed GCS locations to simulate impacts of the structures on flow and infiltration. Our model was able to predict the slight reduction and delay in peak flows for small events and simulate infiltration, which was measured and occurred in the channel. Results demonstrated that structures could increase infiltration by ~15% over time. More data describing geomorphology and hydrology after repeated rainfall events will allow for increased analyses. Innovative monitoring, including the large‐scale particle image velocimetry (LSPIV) were invaluable to this research. Given the arid-land location and added drought conditions, the water levels were not high enough to compute, even using the continuous slope-area method, so discharge was calculated solely using the LSPIV. The careful redundancy of data acquisition is extremely important when studying dryland hydrology. Weather data indicated that the HSP GCS installations created roughly a three-degree microclimate cooling effect for at least two days following rainfall events, as compared with the untreated channel. The cooling was attributed to increased moisture, evaporation, and latent heat expulsion from the evaporation.

Arizona

Population genomic surveys for six rare plant species in San Diego County, California

San Diego County is a hotspot of biodiversity, situated at the intersection of the Baja peninsula, the California floristic province, and the desert southwest. This hotspot is characterized by a high number of rare and endemic species, which persist alongside a major urban epicenter. San Diego County has implemented a strategic management plan that identifies species, based on low numbers of occurrences or high level of threat, for which management practices are recommended. In creating a management plan for rare species, it is important to strike a balance between preserving locally adapted traits and maintaining genetic diversity, as species’ ranges fluctuate in response to a changing climate and habitat fragmentation. This project, in partnership with the San Diego Natural History Museum, aims to provide a reference point for the current status of genetic diversity of rare plant species that will inform future preservation and restoration efforts. We focused on six threatened or endangered plant species: Acanthomintha ilicifolia , Baccharis vanessae , Chloropyron maritimum ssp. maritimum , Deinandra conjugens , Dicranostegia orcuttiana , and Monardella viminea . For each species, botanists from the San Diego Natural History Museum visited all known occurrences in San Diego County and collected leaf tissue for genetic and cytological analysis. We then developed a panel of genetic markers to estimate genetic diversity and population structure. This population genetic survey provided insight into the amount of genetic differentiation across each species’ range, identified isolated occurrences potentially subject to inbreeding or genetic bottlenecks, and identified areas that are rich sources of allelic diversity. Finally, we convened a panel of experts to review results and compatible management options for each species. A summary of the management workshop is included in this report. Overall, we found low genetic differentiation among occurrences across the San Diego region for all species, with the exception of A. ilicifolia . Relative inbreeding was low and consistent across sites, and genetic diversity across sites was variable, with noted exceptions. These findings allow for a wide array of management options that are compatible with panmictic population structure in five of the six surveyed species.

California

Smallmouth buffalo (Ictiobus bubalus) growth across a 1200km human use and ecological disturbance gradient in the Upper Mississippi River System

Smallmouth buffalo (Ictiobus bubalus) is a common and widely distributed large-bodied species of the family Catostomidae. It inhabits large rivers and reservoirs of the eastern continental United States (east of the continental Divide) and is most abundant and common in the large rivers of the Midwest and Central Plains, though it does occur as far north and east as the Hudson Bay drainage and as far south and west as Arizona (Edwards and Twoney 1982). Historically, smallmouth buffalo were an important component of commercial fisheries on both the Mississippi and Illinois Rivers. However, following the introduction of common carp (Cyprinus carpio) in the mid-1800s (Carlander 1954), the construction of a system of navigation dams on Upper Mississippi and Illinois River in the 1930s (USGS 1999), and water quality/pollution issues through the 1980s (Weiner 2010), the role of smallmouth buffalo in the overall UMRS fish community and commercial fishery has generally diminished relative to historical standards. Still, smallmouth buffalo remains an important and valued component of the UMRS commercial fishery. The study area is represented by three study reaches on the Illinois River and three study reaches on the Upper Mississippi River (Figure 1). Collectively, these study reaches represent nearly 1200 river km and exist across strong and pronounced ecological and disturbance gradients. For example, habitat composition, water quality, commercial navigation intensity, aquatic plant prominence, and the number and abundance of nonnative fish species vary strongly across the study domain, with northern Mississippi River reaches exhibiting less navigation traffic, better water quality, markedly greater aquatic plant prominence, more diverse habitat composition, and comparably much smaller numbers of nonnative species than the lower Mississippi River study reach and those on the Illinois River (USGS 1999; Johnson and Hagerty [eds] 2008; Irons et al. 2009). Long term monitoring efforts conducted under the auspices of the Upper Mississippi River Restoration program over the past 27 years have provided tremendous insights into shifts and changes of the overall UMRS fish community (Ickes et al. 2005; Garvey et al. 2010; Schramm and Ickes 2016). However, these monitoring efforts observe only the most basic aspects of the UMRS fish community (i.e., catch, length, weight, distribution, and occurrence). To gain a greater understanding of forces driving community level shifts and changes, more directed study is needed on the functional attributes of fish populations (i.e., growth, mortality, recruitment). Collectively, these functional attributes of populations are termed population dynamics and/or vital rates. It is important to note, the population dynamics of fishes in large rivers is generally poorly understood, especially for non-game species (Ickes 2018). The prevailing view is that abiotic factors largely govern inter-annual population dynamics, typically based upon rather short-term observations and correlations with assorted abiotic river attributes that vary on a seasonal or annual basis (for example, Risotto and Turner 1985). However, the role that longer-term abiotic factors play in regulating population abundance, or that biotic factors internal to the population (e.g., spawner-recruit dynamics, growth dynamics) or external to the population (e.g., predator-prey dynamics, sympatric competitors, disease) remain poorly understood. Achieving a greater understanding of these dynamics is important for stock, game, and invasive species management. In 2017, as part of a larger study designed to gain vital population rate information for smallmouth buffalo in the Upper Mississippi and Illinois Rivers (“Smallmouth Buffalo population demographics of the Upper Mississippi River System”; UMRR LTRM 2018SOW project items 2018MMBF1-2018MMBF6) annual growth patterns in smallmouth buffalo were determined and evaluated. This was accomplished by measuring growth histories recorded in annual growth increments on hard bony parts (here otoliths), a method known generically as biochronology, and somewhat analogous to dendrochronology practiced by foresters. These methods allow one to generate time-series of annual growth histories that depend upon age, year class (i.e., cohort), and annual environmental conditions experienced by the population over time (Weisberg, 1993). Biochronology methods were used to develop a 36-year time series of smallmouth buffalo growth in the Upper Mississippi and Illinois Rivers across a 1200 km ecological and human use disturbance gradient. Annual growth intervals were identified and measured from otoliths to determine fish age and growth history. A mixed model that parses the growth increment into age and year effects was fit to these data. Given the pronounced ecological and disturbance gradients inherent to the UMRS and the study domain, an a priori expectation of differing patterns in growth is accepted as a null hypothesis to test. The goal of this study was to model smallmouth buffalo growth as a function of the age of the fish and the growth year in which the growth was gained. The primary modeling objective was to parse growth observed on each annulus into a portion attributable to the age of the fish and the portion attributable to the year in which the growth was gained. In effect, this modeling approach removes the somewhat trivial age effects on growth so that a non-confounded growth year effect can be gained. Results attributable to growth year provide a time series of growth information that is of the same duration as the oldest fish observed and solely reflects environmental influences on growth. These model responses can then be investigated relative to environmental covariate time-series suspected of influencing growth of smallmouth buffalo in the Upper Mississippi and Illinois Rivers (e.g., temperature, discharge, population density, population mortality, forage availability, sympatric competition, habitat composition, navigation intensity, nonnative fish prominence, etc.). Thus, the primary scientific objective was to investigate if and how smallmouth buffalo growth varies in accordance with innate ecological and disturbance gradients across the study domain.

Illinois, Iowa, Minnesota, Missouri, Wisconsin

Observations of coastal sediment dynamics of the Tijuana Estuary Fine Sediment Fate and Transport Demonstration Project, Imperial Beach, California

Coastal restoration and management must address the presence, use, and transportation of fine sediment, yet little information exists on the patterns and/or processes of fine-sediment transport and deposition for these systems. To fill this information gap, a number of State of California, Federal, and private industry partners developed the Tijuana Estuary Fine Sediment Fate and Transport Demonstration Project ("Demonstration Project") with the purpose of monitoring the transport, fate, and impacts of fine sediment from beach-sediment nourishments in 2008 and 2009 near the Tijuana River estuary, Imperial Beach, California. The primary purpose of the Demonstration Project was to collect and provide information about the directions, rates, and processes of fine-sediment transport along and across a California beach and nearshore setting. To achieve these goals, the U.S. Geological Survey monitored water, beach, and seafloor properties during the 2008–2009 Demonstration Project. The project utilized sediment with ~40 percent fine sediment by mass so that the dispersal and transport of fine sediment would be easily recognizable. The purpose of this report is to present and disseminate the data collected during the physical monitoring of the Demonstration Project. These data are available online at the links noted in the "Additional Digital Information" section. Synthesis of these data and results will be provided in subsequent publications.

California

Sources and characteristics of dissolved organic carbon in the McKenzie River, Oregon, related to the formation of disinfection by-products in treated drinking water

Executive Summary This study characterized the concentration and quality of dissolved organic carbon (DOC) in the McKenzie River, a relatively undeveloped watershed in western Oregon, and its link to forming disinfection by-products (DBPs) in treated drinking water. The study aimed to identify the primary source(s) of DOC in source water for the Eugene Water & Electric Board’s (EWEB) conventional treatment plant on the McKenzie River near river mile 11, upstream of Hayden Bridge. The two classes of regulated compounds examined—trihalomethanes (THMs) and haloacetic acids (HAAs)—form when organic carbon in raw source water reacts with chlorine and (or) bromine during water treatment. The objectives of the study were to: characterize the amount and quality of DOC in the McKenzie River and select tributaries during storms; identify the most common types of carbon using UV-vis spectroscopy and other methods; evaluate optical properties for predicting DBP precursors in surface water; and identify land cover classes or vegetation types that may be important sources of organic carbon and DBP precursors in EWEB’s source water. Eleven storms were sampled synoptically in upstream-to-downstream fashion to provide a “snapshot” of water quality conditions at four sites on the McKenzie River from Frissell Bridge (6 miles downstream from Trail Bridge Reservoir) to the EWEB water treatment plant at Hayden Bridge and nine contributing tributaries. Storms included late summer and early autumn “first flush” events and late autumn, winter, and spring storms spanning a range in streamflows from 3,000 to 26,000 cubic feet per second as measured in the main stem McKenzie River at the EWEB water intake. Water samples were analyzed for DOC concentrations and optical properties (fluorescence and ultraviolet absorbance [UVA]) across a range of wavelengths to characterize the quantity and quality of dissolved organic matter (DOM) in the McKenzie River at the drinking water intake and upstream locations. Paired sets of source and finished water samples were collected at the EWEB treatment plant to identify DOC quality parameters in raw source water that might predict DBP concentrations in finished drinking water. DOC concentrations were relatively low in the McKenzie River (0.4–3 milligrams per liter [mg/L]; average 1.5 mg/L) but much higher in the tributaries. The highest DOC concentrations occurred during “first flush” storms in October 2012 and September 2013; the highest value (16 mg/L) was measured at the 52nd Street stormwater outfall. The average DOC concentration in the lower basin-tributaries was 3.8 mg/L; three middle basin tributaries—Quartz, Gate, and Haagen Creeks, which drain private forestland with less coniferous forest compared with other higher elevation tributaries— had slightly lower average DOC concentrations (2.8 mg/L). These middle-basin watersheds may be important sources of DOC and DBP precursors to the McKenzie River, even more so than the lower basin tributaries, depending on their flows (and loads). This is particularly true after the September 2020 Holiday Farm fire, which burned much of this area. DOC concentrations increased 68 percent in the McKenzie River between the uppermost reference site at Frissell Bridge and Vida; this includes drainage from Quartz Creek, Blue River Lake and Cougar Reservoir, which all contributed DOC to the main stem. In contrast, the lowermost tributaries draining most of the agricultural and urban land did not have a large effect on DOC in the McKenzie River despite their higher DOC concentrations because of their presumed relatively low streamflows and, consequently, DOC loads. Apart from the continuous flow monitors in the McKenzie River and some tributaries (Blue River and South Fork McKenzie River, and streamflow at Hayden Bridge and Vida, Camp Creek and some other locations), streamflow was not assessed during sample collection for this study. This lack of streamflow data precludes a detailed analysis of loads, which is discussed in the future studies section. All DBP concentrations in finished drinking water were less than EPA maximum contaminant levels (MCLs) of 0.080 mg/L for the four trihalomethanes (THM4) and 0.060 mg/L for five haloacetic acids (HAA5). During the 11 storm sampling events the maximum summed concentrations were about 0.040 mg/L for both THM4 and HAA5. Compliance monitoring samples, collected separately by EWEB, yielded some higher concentrations—0.046 mg/L THM4 and 0.047 HAA5—during the December 2012 storm. The corresponding benchmark quotient (BQ) values, which indicate how close a measured DBP concentration is to the MCL, were 0.58 and 0.78, respectively, for THM4 and HAA5. Compared with a similar 2007–08 McKenzie River study that did not target storm events, concentrations of THM4 and HAA5 in finished water were 68 percent and 33 percent higher, respectively, during the current study. Due to the high dilution rates in the McKenzie River main stem, many of the individual fluorescence excitation-emission measurements were low (<0.1 Raman units) and approached analytical detection limits. Parallel factor analysis (PARAFAC) resulted in a five-component model (C1–C5) that represents five unique organic fluorophores. Components C1, C2, and C3 represent DOM associated with soil-derived, humic-like, more degraded organic matter. In contrast, components C4 and C5 represent “fresher” DOM, derived from terrestrial and aquatic plants, including algae and cyanobacteria that are common in the McKenzie River and its tributaries and reservoirs. The fluorescence data and PARAFAC modeling suggest that most of the DOC in the McKenzie River originated from terrestrial sources (primarily components C1 and C2). The largest increases in DOC in the main stem occurred in the reach upstream of Vida, from inflows by Quartz Creek, Blue River, South Fork McKenzie River, and other tributaries. Concentrations of DBPs in EWEB’s finished drinking water were positively correlated with DOC concentrations in raw source water (THM4, p <0.05; HAA5, p <0.01) for paired samples collected 12−24 hours apart. DOC concentrations were significantly positively correlated ( p <0.001) with laboratory-based fluorescent dissolved organic matter (fDOM) measurements, suggesting fDOM as a useful parameter for monitoring and predicting DOC concentration in surface water and DBP concentrations in finished water. Of all the PARAFAC components in surface water, C5 had the highest correlations with DBPs in finished water (rho = 0.77–0.84, p <0.01), followed by components C1 and C2 (rho = 0.75 and 0.71, respectively, p <0.01). This C5 carbon is associated with recently produced DOM, possibly from decomposed terrestrial and aquatic vegetation. Model loadings of these three components were considerably higher in the sampled tributaries relative to the main stem McKenzie River, with most of the observed increases in the main stem apparent at Vida. This points to Quartz Creek or other tributaries in the reach between Frissell Bridge and the sampling site near Vida (South Fork McKenzie and Blue Rivers) as potentially key contributors of DOM source material that leads to the production of DBPs in treated drinking water. A limited load analysis showed that the reservoirs contributed 8–37 percent of the instantaneous DOC loads observed at Vida at the time of sampling, which suggests other sources such as Quartz Creek and other streams in the reach between Frissell Bridge and Vida are more important. Random forest analyses identified PARAFAC components C1 and C5 and fluorescence peaks A, C, M, T and N as the best predictors for HAA5 concentrations in finished drinking water, explaining 62.5 percent of the variation. The best predictors for THM4 were C1, C4 + C5, and peaks T, A, and N, which explained 33 percent of the variation. Several land cover and vegetation classes were correlated with DOC concentration and other optical measurements. The percentage of evergreen forest in each of the subwatersheds sampled was negatively correlated ( p <0.001) with DOC concentration and many optical indicators of DOM quantity: UVA 254 , fDOM, and all of the fluorescence peaks. In contrast, mixed (deciduous) forest was positively correlated ( p <0.001) with DOC, fDOM, UVA 254 , and several fluorescence peaks, demonstrating the importance of deciduous leaf fall in generating DOC and DBP precursors. The high level of human activities in the middle and lower portion of the basin—including timber harvesting and road construction on private forestland, agricultural, rural, industrial, and urban development—have resulted in the greatest loss in native coniferous and mixed deciduous forests in the basin. DOC loading from these tributaries and reservoir releases, which contain DOC from terrestrial and aquatic productivity, both enrich the McKenzie River. Concentrations of DOC increased an average of 71 percent (range 30–120 percent) in the McKenzie River between Frissell Bridge, the upstream reference site, and Vida. PARAFAC components C1, C2, and C5—which were correlated with DBPs in finished water—increased, on average, 109–136 percent (range 20–250 percent) in this same Frissell-to-Vida reach. These increases occur from input of tributaries in the middle basin such as Quartz Creek and others, as noted above. Future monitoring, field, and lab studies can improve our understanding of seasonal and spatial sources of organic carbon contributing DBP precursors to the McKenzie River and allow detection of long-term trends resulting from the recent Holiday Farm Fire, which burned 173,393 acres of forestland, including riparian areas along the main stem, and numerous structures, homes, and outbuildings in September 2020. Future studies could examine DOC fluxes and flushing of carbon from the watershed, investigate the role of precipitation amount and intensity in mobilizing carbon and sediment, and evaluate impacts to aquatic communities and human health as part of a post-fire assessment. Other areas ripe for study include evaluating the impacts of potential temperature increases on carbon sequestration and decomposition in the burned and unburned forests and identifying practices that foster sequestration of carbon in forest soils. The use of fluorescence sensors such as fDOM to monitor the concentration and composition of raw water supplies may be improved for detection of specific DBP precursors, to provide continuous and real-time information to treatment plant operators. Future studies that monitor DOM amount and quality, and DBP Formation Potential (FP), particularly during storm events, paired with streamflow measurements, as suggested above, could help identify areas that contribute high DOC loads and thus help managers identify the key areas to focus restoration activities. Other studies could examine treatment options for currently regulated DBPs and potentially unregulated compounds, including advanced biological treatments for their removal. This study was a collaboration between the U.S. Geological Survey (USGS) and EWEB in Eugene, Oregon, with additional funding provided from USGS Cooperative Matching Funds Program.

Oregon

Community and citizen science on the Elwha River: Past, present, and future

This report reflects on the past, present, and potential future of community and citizen science (CCS) in the Elwha River watershed, with particular focus on the years before and after a major restoration event: the removal of two dams that had impacted the river system for a century. We ask: how does CCS feature in the Elwha story and how could it feature? We use the term CCS to reference the broad range of ways in which members of the public might participate in authentic science and monitoring processes, including students and both paid and unpaid interns: participants are individuals contributing to scientific projects without prior formal training in the topic. Removal of the Elwha dams was a large-scale, complex project, and communities had an important role to play: the Lower Elwha Klallam Tribe (LEKT) and other local groups were a large part of the original drive to remove the dams. Some funding and policy requirements for monitoring are ending, but there is still much to learn from the changes happening in the Elwha, requiring ongoing research and monitoring. In 2022, the Elwha scientific community came together in a multifaceted effort called the “Elwha ScienceScape” to mark the ten-year anniversary of dam removal and to plan for future monitoring. One of ScienceScape’s priorities is expanding CCS efforts, and because the Elwha dam removal is a powerful international symbol of large-scale watershed restoration, ScienceScape is well-positioned to inform and emphasize the potential role of CCS in dam removal worldwide. This report presents insights about Elwha CCS from an academic literature review and discussions with scientists and many others that have been working in the Elwha. We found that the history of CCS on the Elwha is important but understated, with few scientific papers acknowledging support by volunteers of various kinds. Recent and ongoing CCS projects on the Elwha tend to be focused on biological phenomena, and most are associated with educational opportunities (across many types of institutions) and paid internships. We also noted that most Elwha CCS projects required volunteers with particular pre-existing skill sets (e.g., botanical knowledge) or time to impart specialized training (e.g. boat use), leading many projects towards engagement with a smaller number of volunteers. Partners working in the Elwha are considering a wide range of potential new CCS projects, and these ideas are in varying stages of development. Many new projects would broaden public involvement in terms of the opportunities available and increase the variety of focal topics for research and monitoring. This increased breadth is promising: there are indications that the local community’s interests also range widely, from fish recovery after dam removal to dam removal impacts on humans. Elwha CCS projects have encountered some challenges and barriers, including the administrative burden of coordinating volunteers and managing liability concerns. But Elwha ScienceScape scientists are committed to the value that CCS brings both to the research itself as well as to those who participate in these projects. CCS can be a way to increase equity in science and engage people who would not otherwise participate in research, and in many cases the research simply wouldn’t be possible without their help. Support with project administration, volunteer management, and data management could help in expanding CCS efforts and broadening their inclusivity. More systematic tracking of CCS projects to assess how they contribute to research and to community and participant benefit could be helpful in establishing and maintaining a long-term CCS strategy in the Elwha.

Wshington

Long-term monitoring of island night lizards on San Nicolas Island

We describe the results of long-term population monitoring of the island night lizard Xantusia riversiana on San Nicolas Island, California, following the species' removal from the U.S. Endangered Species list in 2014. Monitoring activities were carried out from October 2014 through November 2023, but we also incorporate data from earlier work dating back to 1993. Because of habitat loss on the western part of the island, the island night lizard is almost entirely confined to the island's eastern half. The species' distribution remains largely the same since studies in the 1990s, though small increases in distribution were noted at the island's west end. Numbers at most long-term monitoring sites appeared to show a slight decrease associated with a multi-year drought, with numbers returning to previous levels by the end of the monitoring period. Models fit to counts of newly recruited lizards suggested a positive association between winter precipitation and abundance; however, we found a simpler model including constant abundance throughout the study to be more parsimonious. Two monitoring sites showed marked decreases in numbers, with one site declining to zero following infestation of the area by non-native Argentine ants Linepithema humile . The island's vegetation and natural communities continue to gradually recover from a century of overgrazing by sheep and the widespread introduction of aggressive non-native plants. Habitat restoration efforts by U.S. Navy natural resources staff on the island may lead to increasing population numbers and genetic connectivity, but persistent threats remain for the island night lizard on San Nicolas Island.

California

Sharp savanna-forest transitions in the Midwest followed environmental gradients but are absent from the modern landscape

Historically, closed eastern forests transitioned into open savannas and prairies in the US Midwest, but this transition is poorly understood. To investigate the eastern boundary of the prairie-forest ecotone, we conducted a case study of historic and modern vegetation patterns of the Yellow River watershed in northwest Indiana. Historic vegetation came from the Public Land Survey notes collected in the early 1800s, whereas modern vegetation came from the Forest Inventory Analysis and USGS National Land Cover Database. We mapped historical survey vegetation data using GIS to reconstruct the region’s past and current forest composition and structure. We also mapped climate, topography, and soil composition across the watershed to investigate the relationship between historic vegetation and environmental gradients. We found a sharp transition in the presettlement forest structure and composition, with dense deciduous forests in the eastern portion of our study area and open oak savannas in the west. The savanna ecosystem dominated in sandy well-drained soils and was at a slightly lower elevation than the adjacent closed forest. Modest environmental changes accompanied major vegetation changes in the past, which might suggest fire and hydrological patterns helped maintain the sharp ecotone. By contrast, the modern forest shows no difference in tree density and composition across the watershed, which is consistent with major land use and hydrology changes in the watershed since settlement. On the modern landscape, land that was historically closed forest now has higher agricultural productivity compared to land that was historically savanna, whereas the historic savanna currently supports more mesic forest. These results suggest the environmental gradient continues to subtly shape the landscape. Though land use change has largely removed the closed mixed hardwood forests and oak savannas from this area, a better understanding of the historic vegetation and the conditions that supported it can help inform land management and restoration, as well as reveal ecological processes that drive vegetation transitions.

Indiana

Water-quality and ground-water hydrology of the Columbia/Eagle Bluffs Wetland Complex, Columbia, Missouri— 1992-99

In an effort to restore riverine wetlands along the Missouri River, the Missouri Department of Conservation constructed the 2,700-acre Eagle Bluffs Conservation Area. The primary water source for managing 1,200 wetland acres on the Eagle Bluffs Conservation Area is treated effluent received from a 91-acre constructed wastewater-treatment wetland operated by the city of Columbia, Missouri. The combined areas of the Eagle Bluffs Conservation Area and the wastewater-treatment wetland are termed the Columbia/Eagle Bluffs Wetland Complex. The U.S. Geological Survey, in cooperation with the Missouri Department of Conservation and the city of Columbia, Missouri, collected samples quarterly from August 1992 to March 1999 from a monitoring network that included 33 ground-water sites and 4 surface-water sites to establish a baseline pre-effluent data set and to provide post-effluent data for trend analysis. Changes in major chemical constituent concentrations have been observed at several sampling locations between pre- and post-effluent data. Analysis of post-effluent time-series water-quality data indicates changes occurred in sodium, potassium, calcium, sulfate, and chloride concentrations at 13 sites. These changes can be correlated to the beginning of the operation of the wastewater-treatment wetland. The concentrations of these major chemical constituents plot on the mixing continuum between pre-effluent ground water as one end member and the treated effluent as the other end member. At ground water sites that had changes in concentrations, the relative percentage of treated effluent in the ground water, assuming chloride is conservative, ranged from 11 to more than 100 percent. At ground-water sites, few changes were noted in fecal indicator bacteria, nutrients, trace constituents, total and dissolved organic carbon, and organic constituents. Other than changes in boron concentrations at one ground-water site, these changes could not be directly correlated to the operation of the treatment wetland or the management of the Eagle Bluffs Conservation Area. After the treatment wetland began operation, improvement in the water quality in Perche Creek was observed. With respect to fecal indicator bacteria and nutrient concentrations, the water quality of water discharging from the Eagle Bluffs Conservation Area was improved relative to the water entering the area. Persistent ground-water highs have been observed beneath the Eagle Bluffs Conservation Area and wastewater-treatment unit 1 following the flooding of the wetland areas. These ground-water highs occur during the fall and winter months when ground- and surface-water levels are high and during the spring and summer months when the water levels are lower. The Missouri River stage had a strong effect on the water levels in the aquifer during pre-effluent conditions, but the effect has been lessened by the ground-water high.

Missouri

Sage-grouse

In this chapter, we summarize the ecology and conservation issues affecting greater ( Centrocercus urophasianus ) and Gunnison ( C . minimus ) sage-grouse, iconic and obligate species of rangelands in the sagebrush ( Artemisia spp.) biome in western North America. Greater sage-grouse are noted for their ability to migrate, whereas Gunnison sage-grouse localize near leks year-round. Seasonal habitats include breeding habitat where males display at communal leks, nesting habitat composed of dense sagebrush and herbaceous plants to conceal nests, mesic summer habitats where broods are reared, and winter habitat, characterized by access to sagebrush for cover and forage. While two-thirds of sage-grouse habitat occurs on public lands, private land conservation is the focus of national groups including the USDA-NRCS Sage-Grouse Initiative. Sage-grouse are a species of great conservation concern due to population declines associated with loss and fragmentation of more than half of the sagebrush biome. Wildlife and land management agencies have been increasingly proactive in monitoring trends in sage-grouse populations (e.g., lek count index), adapting regulations to reduce harvest on declining populations, and in designing and implementing conservation policies such as core areas to conserve sage-grouse habitats and populations. Much of the remaining sagebrush habitat is threatened by altered fire regimes, invasive annual grasses and noxious weeds, encroaching piñon ( Pinus edulis and monophylla )-juniper ( Juniperus spp.) woodlands, sagebrush conversion, anthropogenic development, and climate change. Several diseases affect sage-grouse, but to date, disease has not been a widespread cause of declines. Proper livestock grazing and limited hunting appear to be sustainable with sage-grouse, whereas improper grazing, increasing free-roaming equid populations, and sagebrush conversion are primary concerns for future conservation. Research has identified additional concerns for sage-grouse including effects from fence collisions, predation from common ravens ( Corvus corax ), and reduced habitat effectiveness resulting from grouse avoidance of anthropogenic infrastructure. There is a need for future research evaluating sage-grouse habitat restoration practices following improper rangeland management, habitat alteration from invasive species and fire, effects on small and isolated populations, and effects from diseases.

Book chapter

Fishes of Missouri River, chute, and flood plain habitats: Chapter 4 in Initial biotic survey of Lisbon Bottom, Big Muddy National Fish and Wildlife Refuge

The Lisbon Bottom Unit of the Big Muddy National Fish and Wildlife Refuge (Refuge) is approximately 2,200 acres and is the first complete unit of the Refuge. Primary objectives of the Refuge are to create and restore diverse riverine aquatic habitats and reconnect the Missouri River to its flood plain where feasible. Management seeks to accomplish these objectives by encouraging natural processes of erosion, deposition, and succession to the greatest extent possible. One of the most salient aquatic features of the Lisbon Bottom Unit is a newly created 2-mile-long free-flowing chute, or side channel (Fig. 1). This chute began forming as a levee breech scour hole during the Great Flood of 1993. The chute continued to develop during the 1995 flood and finally cut through to a flowing side channel during the 1996 flood. Extensive erosion and bank sluffing continued during 1997 due to sustained high flows that occurred throughout most of the year. The chute has progressively become wider and deeper with a developing meander pattern and channel bars have begun to form. Lisbon Bottom also contains several seasonal and permanent wetlands and is subject to periodic flooding at high Missouri River stages. Eight studies have been completed or are ongoing to evaluate Missouri River fishes associated with various habitat components of Lisbon Bottom and adjacent Missouri River reaches (Table 1). Several are part of much larger investigations to evaluate fish use of flood-created habitat features, basinwide fish assessment, and endangered or candidate species concerns. At the Lisbon Bottom Unit or in the Missouri River adjacent to the unit 54 fish species were collected (Table 2). Eight of these species have either a protected status under State or Federal laws or biologists consider them to potentially qualify for protected status. The status of the following fish is listed in Table 2: pallid sturgeon x shovelnose sturgeon hybrid, paddlefish, northern pike, sturgeon chub, sicklefin chub, ghost shiner, western silvery minnow, plains minnow, and blue sucker. Equally important to note are fish species that were not collected. The flathead chub, currently listed as State endangered and proposed for Federal listing, were not collected in any of the studies. Common fish species, collected in all seven sampling studies, included gizzard shad, common carp, river carpsucker, and freshwater drum. Paddlefish, skipjack herring, silver carp, white sucker, redfin shiner, western silvery minnow, bullhead minnow, and black bullhead were each collected in only one of the seven studies. Pflieger (1971) developed a guild system for the fishes of Missouri based on distribution patterns and centers of abundance. Fish were assigned to one of four primary faunal groups: Ozark, Big River, Lowland, and Prairie. Two secondary groups (Ozark-prairie and Ozark-lowland) were defined for species equally abundant and distributed in two of the primary areas. Two Ozark-prairie species, shorthead redhorse and white sucker, were collected on or near the Lisbon Bottom Unit. Big River species are found primarily in the Missouri and Mississippi Rivers. The environmental factors controlling fish distribution in these rivers appear to be substrate, current velocity, and turbidity. On or near the Lisbon Bottom Unit 18 Big River species were collected (Table 2). Lowland species are intolerant of siltation and high turbidity. They inhabit standing or slow-moving water with sand, fine gravel, and organic debris substrates. Two Lowland species, bullhead minnow and mosquitofish, were collected in connected scours on the Lisbon Bottom Unit. Prairie species have broader ecological tolerances than the Ozark and Lowland species. They are largely absent in high gradient streams and cool, clear waters. Prairie species make up a significant proportion of Missouri and Mississippi River fishes. Seven Prairie species were collected on or near the Lisbon Bottom Unit (Table 2). The last group, Wide-ranging, was defined as the species more widespread than the other faunal groups with broader environmental tolerances. Most Wide-ranging species occur at least occasionally in all sections of the state. Nineteen Wide-ranging species were collected on or near the Lisbon Bottom Unit (Table 2). Kubisiak (1997) found that isolated scours in the Lower Missouri River were dominated by Wide-ranging species. Connected scours also held Wideranging species but they contained Prairie species and were dominated by Big River species. Faunal group diversity and species richness were compared between isolated (Study l) and connected (Studies 4, 5, 7, and 8) scours of the Lisbon Bottom Unit. Species richness was similar with 22 species collected in isolated scours and an average of 24.8 species collected in connected scours. Isolated scour fish catches were dominated by Wide-ranging fish (59%) with 14% Big River fishes and 14% Prairie fishes. Connected scours had a larger percentage of Big River fish (32%) and fewer Wide-ranging (36%) and Prairie (13%) species than the isolated scours. In the combined studies, Wide-ranging fish dominated the connected scours. A similar comparison was made of fish from chute habitats and the Missouri River. Chute habitats include the Lisbon Bottom Chute, the chute between the right bank and island located at river mile 219, and a shallow channel running between a large sandbar complex and the channel border adjacent to Lisbon Bottom. Species richness in the chutes (35 species) was greater than that of the adjacent river (23 species). Big River species (40%) dominated the chute samples, followed by Wide-ranging at 31% Cand Prairie Cut 14%. The river samples contained equal numbers of Wide-ranging species (35%) and Big River species (35%) with 17% Prairie species. Differences in fish guild diversity and species richness may be due to numerous factors including sampling effort and sampling season. River samples were collected only in Study 3 in October; chute samples were collected in July, August, and October. Fish may have been using the chute in July and August to spawn, feed, or escape high river levels. The Lisbon Bottom Chute is a truly unique feature of the Lower Missouri River. It is the first naturally created side channel to develop since the U.S. Army Corps of Engineers tamed the river. The Corps eliminated 89% of the islands in the Missouri River between 1879 and 1954 (Funk and Robinson 1974). The chutes between the islands and the shore were shallower with less current than the main channel. These areas provided diversity to the Missouri River fish habitat, serving as nursery and feeding areas. The Lisbon Bottom Unit of the Big Muddy National Fish and Wildlife Refuge will continue to be studied in an effort to understand the complex role these areas play in river systems.

Missouri

Final report: Initial ecosystem response of salt marshes to ditch plugging and pool creation: Experiments at Rachel Carson National Wildlife Refuge (Maine)

This study evaluates the response of three salt marshes, associated with the Rachel Carson National Wildlife Refuge (Maine), to the practice of ditch plugging. Drainage ditches, originally dug to drain the marsh for mosquito control or to facilitate salt hay farming, are plugged with marsh peat in an effort to impound water upstream of the plug, raise water table levels in the marsh, and increase surface water habitat. At two study sites, Moody Marsh and Granite Point Road Marsh, ditch plugs were installed in spring 2000. Monitoring of hydrology, vegetation, nekton and bird utilization, and marsh development processes was conducted in 1999, before ditch plugging, and then in 2000 and 2001 (all parameters except nekton), after ditch plugging. Each study site had a control marsh that was monitored simultaneously with the plugged marsh, and thus, we employed a BACI study design (before, after, control, impact). A third site, Marshall Point Road Marsh, was plugged in 1998. Monitoring of the plugged and control sites was conducted in 1999 and 2000, with limited monitoring in 2001, thus there was no ?before? plug monitoring. With ditch plugging, water table levels increased toward the marsh surface and the areal extent of standing water increased. Responding to a wetter substrate, a vegetation change from high marsh species (e.g., Spartina patens) to those more tolerant of flooded conditions (e.g., Spartina alterniflora) was noted at two of the three ditch plugged sites. Initial response of the nekton community (fishes and decapod crustaceans) was evaluated by monitoring utilization of salt marsh pools using a 1m2 enclosure trap. In general, nekton species richness, density, and community structure remained unchanged following ditch plugging at the Moody and Granite Point sites. At Marshall Point, species richness and density (number of individuals per m2) were significantly greater in the experimental plugged marsh than the control marsh (<2% of the control marsh was open water habitat vs. 11% of the plugged marsh). The response of birds, categorized as waterfowl & waterbirds, shorebirds & wading birds, gulls & terns, and miscellaneous (raptors, passerines, other), was variable. Following ditch plugging, bird species richness increased at the Granite Point site (1999 pre-plug = 15.4, 2000 post-plug = 26.2, 2001 post-plug = 38.7). Because of a low sample size at Moody Marsh, reliable statements on species richness cannot be made. Density of birds (no. of birds per ha) remained unchanged with ditch plugging at Granite Point Marsh, although there was a strong, but not statistically significant, trend toward increased density. This study only reports on initial responses of marsh functions to ditch plugging. Monitoring should continue at these sites, and perhaps at additional sites, for the next decade or so. A monitoring plan is recommended. Long-term monitoring will include evaluation of salt marsh development processes using SET (surface elevation table) methodology. There is concern, although not confirmed, that as ditch-plugged marshes become wetter and marsh grass production declines their ability to keep pace with sea level rise could be jeopardized. It is suggested that ditch plugging should be considered an experimental marsh management technique. Additional monitoring on the physical and habitat responses of ditch-plugged marshes is required, along with assessments of other techniques aimed at restoring open water habitat to the marsh surface.

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