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Estimating pesticide burdens of bats from guano analyses

Bat guano was first collected and analyzed for environmental pollutants in the early 1970's (REIDINGER 1972:53-64; PETIT & ALTENBACH 1973). Later analyses of both guano and bats from two roosts indicated a correspondence between levels of organochlorine residues in guano and in bat carcasses from the same colony (CLARK & PROUTY 1976). Gray myotis ( mytosis grisescens ) were found dead with lethal brain levels of dieldrin in two colonies where dieldrin levels in guano were high relative to other colonies (CLARK et al. 1978, 1980). We collected and analyzed both guano samples and bats found dead or killed accidentally during handling from numerous roosts of several bat species. The objective of this study was to determine whether a correlation existed between organochlorine residues in guano and in bat carcasses.

Bulletin of Environmental Contamination and Toxico

Brain cholinesterase activity in fledgling starlings: Implications for monitoring exposure of songbirds to ChE inhibitors

Exposure of songbirds to organophosphate and carbamate pesticides is often determined by comparing the brain cholinesterase (ChE) activity of individuals that have been exposed with that of unexposed birds of the same species (e.g., see Busby et al. 1981, 1982; Hamilton et al. 1981). Knowledge of the normal pattern of ChE development in the brains of nestling and fledgling passerines is a necessary prerequisite for monitoring exposure to ChE inhibitors, and assessing the impacts of these inhibitors on the reproductive success and survival of songbirds. In a previous study, Grue et al. (1981) reported that ChE activity in the brains of wild nestling European starlings (Sturnus vulgaris), 3 to 18 days old, varied significantly with age and appeared to increase toward adult levels at a constant rate. Grue et al. (1981) hypothesized that if brain ChE activity continued to develop at a constant rate, levels in the brains of fledgling starlings about 28 days old would be comparable to those in the brains of adults. The present study was conducted to test this hypothesis.

Bulletin of Environmental Contamination and Toxico

Unweathered and weathered aviation kerosine: Chemical characterization and effects on hatching success of duck eggs

Unweathered crude and refined oils are known to be very toxic to the embryos of aquatic birds (ALBERS 1977, 19781 SEARO et ale 1978, WHITE et al. 1979, McGILL & RICHMOND 1979, and others) but the toxicity of weathered petroleum is not as well established. The toxicity of Prudhoe Bay crude oil and No. 2 fuel oil to embryos of mallard ducks (Anas platyrhynchos) decreased only after 3 weeks and 2 weeks of weathering on a large container of fresh water (SZARO et al. 1980). Ten microliters (uL) of 4 week-old Libyan crude oil caused a significant increase in mortality among embryos of Louisiana herons (Hydranassa tricolor) but the same amount of fresh oil did not cause a significant increase in mortality. However, embryos of laughing gulls (Larus atricilla) were not significantly affected by I0 uL of 4 or 8 week-old weathered Libyan crude oil nor i0 UL of fresh oil (MACKO& KING 1980). Crude ell (i0 uL) recovered from the water surface (age unknown, but presumably slightly weathered) near the IXTOC-I oil spill site in the Gulf of Mexico did not significantly reduce the survival of mallard embryos by day 18 of incubation (D. HOFFMAN, unpublished data).

Bulletin of Environmental Contamination and Toxico

DDT poisoning in a Cooper's hawk collected in 1980

In April 1980, a Cooper's hawk ( Accipiter cooperii ) was found on the ground in Lakewood, Colorado, unable to fly and in convulsion. The bird died shortly thereafter. The hawk was packed in dry ice and shipped air express to the Fish and Wildlife Service, U. S. Department of the Interior, National Wildlife Health Laboratory, Madison, Wisconsin, for necropsy. Following necropsy, the brain, gastrointestinal tract, and remaining carcass except skin, feet, wings, liver, and kidney were packed in dry ice and shipped air express to the Patuxent Wildlife Research Center, Laurel, Maryland, for chemical residue analysis. Because the bird's behavior before death suggested some form of poisoning, the kidney was assayed for thallium, the liver for lead, and the gastrointestinal tract for strychnine, sodium fluoroacetate, and arsenic. When these assays proved negative, the bird was analyzed for organochlorine pesticides. Necropsy findings and pesticide residue analyses are reported here.

Colorado

Parathion alters incubation behavior of laughing gulls

One member of each pair of incubating laughing gulls at 9 nests was trapped, orally dosed with either 6 mg/kg parathion in corn oil or corn oil alone, and marked about the neck with red dye. Each nest was marked with a numbered stake and the treatment was recorded. A pilot study with captive laughing gulls had determined the proper dosage of parathion that would significantly inhibit their brain AChE activity (about 50% of normal) without overt signs of poisoning. After dosing, birds were released and the nests were observed for 21/2 days from a blind on the nesting island. The activities of the birds at each marked nest were recorded at 10-minute intervals. Results indicated that on the day of treatment there was no difference (P>0.05, Chi-square test) in the proportion of time spent on the nest between treated and control birds. However, birds dosed with 6 mg/kg parathion spent significantly less time incubating on days 2 and 3 than did birds receiving only corn oil. By noon on the third day, sharing of nest duties between pair members in the treated group had approached normal, indicating recovery from parathion intoxication. These findings suggest that sublethal exposure of nesting birds to an organophosphate (OP) insecticide, such as parathion, may result in decreased nest attentiveness, thereby making the clutch more susceptible to predation or egg failure. Behavioral changes caused by sublethal OP exposure could be especially detrimental in avian species where only one pair member incubates or where both members are exposed in species sharing nest duties.

Texas

Organochlorine concentrations, whole body weights, and lipid content of black skimmers wintering in Mexico and in south Texas, 1983

Since 1978, a large proportion of the black skimmers (Rynchops niger) nesting along the Texas coast have had high concentrations of DDE in their eggs. Of 284 eggs collected from 5 sites during 1978-81, 99.5% contained detectable (> 0.i0 ppm) w levels of DDE, ranging up to 86 ppm wet-weight; 36% of the eggs contained > i0 ppm DDE (White et al., 1984). During the 4-year period, geometric mean concentrations of DDE remained strikingly similar, with no significant decline in egg contaminant levels. The sources of DDE contamination in skimmers are unknown. Recoveries of juvenile skimmers banded in Texas, Louisiana, and Mississippi indicate that a large proportion of the birds (60%) spend the winter on the east and west coasts of Mexico. Therefore, it is possible that the DDE exposure to Texas skimmers occurs in Mexico during the winter, although some birds remain along the Gulf Coast year-round.\ This study was conducted to determine the degree of DDE and other organochlorine contamination in black skimmers and their food items from sites in Mexico and Texas, and to statistically compare residue concentrations and body condition in individuals from the 2 areas.

Texas, Veracruz

Effect of retorted-oil shale leachate on a blue-green alga (Anabaena flos-aquae)

In the event of the development of the large oil shale reserves of Colorado, Utah, and Wyoming, one of the main environmental concerns will be disposal of retorted-oil shale which will be generated in greater volume than the original volume oI the mined oil shale. Investigators have found that leachates of retorted-oil shale are alkaline and have large concentrations of dissolved solids, molybdenum, boron, and fluoride (STOLLENWERK & RUNNELS 1981). STOLLENWERK & RUNNELS (1981) concluded that drainage from waste shale piles could have deleterious effects on the water quality of streams in northwestern Colorado.

Bulletin of Environmental Contamination and Toxico