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Gas-liquid chromatographic determination of kepone in field-collected avian tissues and eggs

C. J. Stafford·W. L. Reichel·D. M. Swineford·R. M. Prouty·M. L. Gay
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Abstract

A procedure is described for determining Kepone (decachlorooctahydro-l,3,4-metheno-2H-cyclobuta [cd]pentalene-2-one) residues in avian egg, liver, and tissue. Samples were extracted with benzene-isopropanol, and the extract was cleaned up with fuming H 2 SO 4 -concentrated H 2 SO 4 . Kepone was separated from organochlorine pesticides and polychlorinated biphenyls on a Florisil column and analyzed by electron capture gas-liquid chromatography GLC). The average recovery from spiked tissues was 86%. The analyses performed on 14 bald eagle carcasses and livers, 3 bald eagle eggs, and 14 osprey eggs show measurable levels which indicate that Kepone accumulates in the tissues of fish-eating birds. Residues were confirmed by GLC-mass spectrometry.

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C. J. Stafford, W. L. Reichel, D. M. Swineford, R. M. Prouty, M. L. Gay. 2020-01-22. Gas-liquid chromatographic determination of kepone in field-collected avian tissues and eggs. https://doi.org/10.1093/jaoac%2F61.1.8

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