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USGS · 1003223

Gas-liquid chromatographic determination of 3-trifluoromethyl-4-nitrophenol residues in fish

J. L. Allen·J.B. Sills
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Abstract

A procedure for the determination of 3-trifluormethyl-4-nitrophenol (TFM) in fish tissues is described. Homogenized tissues are extracted with hexane-ethyl ether; the extract is cleaned up by partitioning the TFM from the extracting solvent into 0.1 N NaOH, acidifying the aqueous solution, and partitioning again with hexaneethyl ether. The TFM is methylated with diazomethane and analyzed by gas-liquid chromatography, using electron capture detection. Recoveries ranged from 75 to 100% from fish muscles that were spiked with 0.01—2.00 μg TFM/g.

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J. L. Allen, J.B. Sills. 2020-02-13. Gas-liquid chromatographic determination of 3-trifluoromethyl-4-nitrophenol residues in fish. https://doi.org/10.1093/jaoac%2F57.2.387

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