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Gas-liquid chromatographic determination of Bayer 73 in fish, aquatic invertebrates, mud, and water

C.W. Luhning·P.D. Harman·J.B. Sills·V. K. Dawson·J. L. Allen
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Abstract

A gas-liquid chromatographic (GLC) method is described for determining residues of Bayer 73 (2-aminoethanol salt of 2’,5-dichloro-4’-nitrosalicylanilide) in fish muscle, aquatic invertebrates, mud, and water by analyzing for 2-chloro-4-nitroaniline (CNA), a hydrolysis product of Bayer 73. Bayer 73 residues are extracted from fish muscle tissue, invertebrates, and mud with acetone-formic acid (98+2), and partitioned from water samples with chloroform. After sample cleanup by solvent and acid-base partitioning, the concentrated extract is hydrolyzed with 2 N NaOH and H 2 O 2 for 10 min at 95°C. The CNA is then partitioned into hexane-ethyl ether (7+3) and determined by electron capture GLC. Average recoveries were 88% for fish, 82% for invertebrates, 82% for mud, and 98% for water at 3 or more fortification levels.

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C.W. Luhning, P.D. Harman, J.B. Sills, V. K. Dawson, J. L. Allen. 2020-01-22. Gas-liquid chromatographic determination of Bayer 73 in fish, aquatic invertebrates, mud, and water. https://doi.org/10.1093/jaoac%2F62.5.1141

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