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Research about Minnesota

Source-linked reports with geographic coverage including Minnesota.

At least 235 records · Page 13Linked to original sources

Relating subsurface temperature changes to microbial activity at a crude oil-contaminated site

Crude oil at a spill site near Bemidji, Minnesota has been undergoing aerobic and anaerobic biodegradation for over 30 years, creating a 150–200 m plume of primary and secondary contaminants. Microbial degradation generates heat that should be measurable under the right conditions. To measure this heat, thermistors were installed in wells in the saturated zone and in water-filled monitoring tubes in the unsaturated zone. In the saturated zone, a thermal groundwater plume originates near the residual oil body with temperatures ranging from 2.9 °C above background near the oil to 1.2 °C down gradient. Temperatures in the unsaturated zone above the oil body were up to 2.7 °C more than background temperatures. Previous work at this site has shown that methane produced from biodegradation of the oil migrates upward and is oxidized in a methanotrophic zone midway between the water table and the surface. Enthalpy calculations and observations demonstrate that the temperature increases primarily result from aerobic methane oxidation in the unsaturated zone above the oil. Methane oxidation rates at the site independently estimated from surface CO 2 efflux data are comparable to rates estimated from the observed temperature increases. The results indicate that temperature may be useful as a low-cost measure of activity but care is required to account for the correct heat-generating reactions, other heat sources and the effects of focused recharge.

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H7N9 influenza A virus in turkeys in Minnesota

Introductions of H7 Influenza A virus (IAV) from wild birds into poultry have been documented worldwide, resulting in varying degrees of morbidity and mortality. H7 IAV infection in domestic poultry has served as a source of human infection and disease. We report the detection of H7N9 subtype IAV in Minnesota turkey farms during 2009 and 2011. The full-genome was sequenced from eight isolates as well as the hemagglutinin (HA) and neuraminidase (NA) gene segments of H7 and N9 virus subtypes for 108 isolates from North American wild birds between 1986 and 2012. Through maximum likelihood and coalescent phylogenetic analyses, we identified the recent H7 and N9 IAV ancestors of the turkey-origin H7N9 IAV, estimated the time and geographic origin of the ancestral viruses, and determined the relatedness between the 2009 and the 2011 turkey-origin H7N9 IAV. Analyses supported that the 2009 and the 2011 viruses were distantly related genetically, suggesting that the two outbreaks arose from independent introduction events from wild birds. Our findings further support that the 2011 MN turkey-origin H7N9 virus was closely related to H7N9 IAV isolated in poultry in Nebraska during the same year. Although the precise origin of the wild-bird donor of the turkey-origin H7N9 IAV could not be determined, our findings suggest that, for both the NA and HA gene segments, the MN turkey-origin H7N9 viruses were related to viruses circulating in wild birds between 2006 and 2011 in the Mississippi flyway.

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Patterns in diurnal airspace use by migratory landbirds along an ecological barrier

Migratory bird populations and survival are affected by conditions experienced during migration. While many studies and conservation and management efforts focus on terrestrial stoppage and staging areas, the aerial environment through which migrants move also is subjected to anthropogenic impacts with potential consequences to migratory movement and survival. During autumn migration, the northern coastline of Lake Superior acts as an ecological barrier for many landbirds migrating out of the boreal forests of North America. From 24 observation points, we assessed the diurnal movements of birds throughout autumn migration, 2008-2010, within a 210 km by 10 km coastal region along the northern coast of Lake Superior. Several raptor species showed patterns in airspace associated with topographic features such as proximity to the coastline and presence of ridgelines. Funneling movement, commonly used to describe the concentration of raptors along a migratory diversion line that either prevents or enhances migration progress, occurred only for Bald and Golden Eagles. This suggests a "leaky" migration funnel for most migratory raptors (e.g., migrating birds exiting the purported migration corridor). Passerines migrating during the late season showed more spatial and temporal structure in airspace distribution than raptors, including funneling and an association with airspace near the coast. We conclude that a) the diurnal use of airspace by many migratory landbirds is patterned in space and time, b) autumn count sites situated along ecological barriers substantially underestimate the number of raptors due to 'leakage' out of these concentration areas, and c) the magnitude and structure of diurnal passerine movements in airspace have been overlooked. The heavy and structured use of airspace by migratory landbirds, especially the airspace associated with anthropogenic development (e.g., buildings, towers, turbines) necessitates a shift in focus to airspace management and conservation attention for these animals.

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Efficacy of Pseudomonas fluorescens strain CL145A spray dried powder for controlling zebra mussels adhering to native unionid mussels within field enclosures

The efficacy of a commercially prepared spray dried powder (SDP) formulation of Pseudomonas fluorescens (strain CL145A) was evaluated for removing zebra mussels ( Dreissena polymorpha ) adhering to a population of unionid mussels in Lake Darling (Alexandria, Minnesota). Two groups of unionid mussels were used in the study. Unionid mussels were collected near the test area, weighed, photographed, individually tagged, and randomly allocated to one of nine test enclosures in equal proportions and then divided into two groups. The first group of unionid mussels (Group 1, n = 5 per test enclosure) were indiscriminately selected from each test enclosure and used to estimate the number of zebra mussels adhering to unionid mussels prior to exposure. The second group of unionid mussels (Group 2, n = 22 per test enclosure) were used to evaluate the efficacy of SDP for removal of adhering zebra mussels. Both Group 1 and Group 2 mussels were used to evaluate the effects of SDP exposure on unionid mussel survival. Treatment was assigned to each test enclosure by using a randomized block design. The three treatment groups were tested in triplicate and included an untreated control group and groups that received a single application of 50 or 100 milligrams per liter (mg/L) of SDP based on active ingredient. All treatment concentrations are reported as active ingredient of SDP. Test enclosures were removed at the 8-hour exposure termination. Both Group 1 and Group 2 mussels remained in their assigned exposure location during the postexposure holding period. The number of zebra mussels adhering to Group 2 mussels (live and dead) was assessed 18 to 20 days postexposure in addition to assessing the survival of Group 1 and Group 2 unionid mussels. SDP, administered as a single treatment, significantly ( p < 0.01) reduced the number of adhering zebra mussels when compared to the untreated controls. The number of zebra mussels adhering to unionid mussels (Group 2) was reduced 53 percent in the 50-mg/L treatment group and 68 percent in the 100-mg/L treatment group. The number of adhering zebra mussels did not differ ( p = 0.79) between the 50- and 100-mg/L treatment groups after exposure. When standardized to the amount of SDP applied per square meter, each gram (g) of SDP applied in the 50-mg/L treatment reduced the number of adhering zebra mussel 59.8 percent more than the 100-mg/L treatment group. Group 1 mussel survival did not differ between treatment groups ( p > 0.05); however, a difference was detected ( p < 0.01) in the survival of Group 2 mussels. The survival of Group 2 mussels did not differ ( p > 0.23) between control and treated groups. A difference in Group 2 mussel survival was detected ( p = 0.03; odds ratio [OR] = 0.290) between the 50- and 100-mg/L treatment groups (that is, the survival was highest in the 50-mg/L treatment group and lowest in the 100-mg/L treatment group), however, the biological significance of the difference is indeterminate.

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Assessment of aquifer properties, evapotranspiration, and the effects of ditching in the Stoney Brook watershed, Fond du Lac Reservation, Minnesota, 2006-9

The U.S. Geological Survey, in cooperation with the Fond du Lac Band of Lake Superior Chippewa, assessed hydraulic properties of geologic material, recharge, and evapotranspiration, and the effects of ditching on the groundwater resources in the Stoney Brook watershed in the Fond du Lac Reservation. Geologic, groundwater, and surface-water data were collected during 2006&ndash;9 to estimate hydrologic properties in the watershed. Streamflow and groundwater levels in the shallow glacial deposits in the Stoney Brook watershed were analyzed to estimate groundwater-flow directions, groundwater recharge, and evapotranspiration within the watershed and to assess the effect of ditches on surrounding groundwater resources. Groundwater, streamflow, and precipitation data collected during the study (2006&ndash;9) can be used to update the U.S. Department of Agriculture&rsquo;s Natural Resource Conservation Service and Fond du Lac Resource Management Division surface-water models, which are used to evaluate the effect of proposed adjustments to the ditching system on streamflow on wild rice production and aquatic habitats. Specific yields calculated from the well water levels ranged from 0.11 to 0.40, and hydraulic conductivities determined from water levels measured during well slug tests ranged from 1 to 7 feet per day. The values for specific yields were similar to values obtained in other studies done in glacial materials of similar composition in Minnesota. The higher hydraulic conductivity estimate (7 feet per day) was similar to lower hydraulic conductivities estimated in another hydrologic study conducted in Carlton County, Minnesota. The installation of drainage ditches in the Stoney Brook watershed has reduced water levels in lakes connected to the ditch system, and has locally reduced groundwater levels in shallow groundwater adjacent to the ditches and lakes. Differences in near-ditch groundwater hydrographs relative to far-ditch groundwater hydrographs indicate that the effect of the ditches on groundwater is only localized to near-ditch areas. These hydrograph differences resulted in large differences between recharge estimated at wells near and far from ditches. In this study, recharge estimated at wells within 50 feet of a ditch was influenced by ditch-water levels. Annual groundwater recharge estimates from water levels and streamflows during 2006&ndash;9 ranged from 0.36 to 34.8 inches, and varied with climate, geology, and well location relative to ditches. The higher recharge estimates were determined from analysis of groundwater levels in wells near the ditches because the shallow groundwater in these wells received both infiltration from ditches and areal groundwater recharge from precipitation. The water-table fluctuation method using a manual groundwater recession approach for wells far from ditches provided the best estimates of areal groundwater recharge to the shallow glacial aquifer because water levels in these wells were not affected by water infiltrating from ditches (bank storage). For wells more than 400 feet from ditches, mean annual areal groundwater recharge estimates using the manual groundwater recession approach for wells screened mostly in outwash sands during 2007, 2008, and 2009 ranged from 4.47 to 18.6 inches (wells 5, 7, 13, 14 and 15), and ranged from 0.43 to 2.85 inches for wells screened mostly in clayey sand or sandy clay (wells 9 and 16). Recharge estimates at wells far from ditches were similar to basinwide recharge estimates from streamflow. Daily fluctuations in water levels in two wells indicated that the evapotranspiration extinction depth in the Stoney Brook watershed is approximately 4.6 to 6 feet below the land surface. A polynomial regression fit of the daily evapotranspiration rates during 2006&ndash;9 for well 1 produced a total evapotranspiration estimate of 16.1 inches from June 26 to October 6 for every year. Evapotranspiration estimated from daily water-level fluctuations in wells near ditches is relatively high. The ditch-water surface allowed for relatively high evaporation compared to the land surface, which, with a good hydraulic connection to surrounding groundwater, resulted in relatively high fluctuations in daily groundwater levels near ditches, resulting in high evapotranspiration estimates.

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An assessment of two methods for identifying undocumented levees using remotely sensed data

Many undocumented and commonly unmaintained levees exist in the landscape complicating flood forecasting, risk management, and emergency response. This report describes a pilot study completed by the U.S. Geological Survey in cooperation with the U.S. Army Corps of Engineers to assess two methods to identify undocumented levees by using remotely sensed, high-resolution topographic data. For the first method, the U.S. Army Corps of Engineers examined hillshades computed from a digital elevation model that was derived from light detection and ranging (lidar) to visually identify potential levees and then used detailed site visits to assess the validity of the identifications. For the second method, the U.S. Geological Survey applied a wavelet transform to a lidar-derived digital elevation model to identify potential levees. The hillshade method was applied to Delano, Minnesota, and the wavelet-transform method was applied to Delano and Springfield, Minnesota. Both methods were successful in identifying levees but also identified other features that required interpretation to differentiate from levees such as constructed barriers, high banks, and bluffs. Both methods are complementary to each other, and a potential conjunctive method for testing in the future includes (1) use of the wavelet-transform method to rapidly identify slope-break features in high-resolution topographic data, (2) further examination of topographic data using hillshades and aerial photographs to classify features and map potential levees, and (3) a verification check of each identified potential levee with local officials and field visits.

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Potential groundwater recharge for the State of Minnesota using the Soil-Water-Balance model, 1996-2010

Groundwater recharge is one of the most difficult components of a water budget to ascertain, yet is an important boundary condition necessary for the quantification of water resources. In Minnesota, improved estimates of recharge are necessary because approximately 75 percent of drinking water and 90 percent of agricultural irrigation water in Minnesota are supplied from groundwater. The water that is withdrawn must be supplied by some combination of (1) increased recharge, (2) decreased discharge to streams, lakes, and other surface-water bodies, and (3) removal of water that was stored in the system. Recent pressure on groundwater resources has highlighted the need to provide more accurate recharge estimates for various tools that can assess the sustainability of long-term water use. As part of this effort, the U.S. Geological Survey, in cooperation with the Minnesota Pollution Control Agency, used the Soil-Water-Balance model to calculate gridded estimates of potential groundwater recharge across Minnesota for 1996‒2010 at a 1-kilometer (0.621-mile) resolution. The potential groundwater recharge estimates calculated for Minnesota from the Soil-Water Balance model included gridded values (1-kilometer resolution) of annual mean estimates (that is, the means for individual years from 1996 through 2010) and mean annual estimates (that is, the mean for the 15-year period 1996&minus;2010). The Soil-Water-Balance model uses a modified Thornthwaite-Mather soil-water-balance approach, with components of the soil-water balance calculated on a daily basis. A key advantage of this approach includes the use of commonly available geographic information system data layers that incorporate land cover, soil properties, and daily meteorological data to produce temporally and spatially variable gridded estimates of potential recharge. The Soil-Water-Balance model was calibrated by using a combination of parameter estimation techniques, making manual adjustments of model parameters, and using parameter values from previously published Soil-Water-Balance models. Each calibration simulation compared the potential recharge estimate from the model against base-flow estimates derived from three separate hydrograph separation techniques. A total of 35 Minnesota watersheds were selected for the model calibration. Meteorological data necessary for the model included daily precipitation, minimum daily temperature, and maximum daily temperature. All of the meteorological data were provided by the Daymet dataset, which included daily continuous surfaces of key climatological data. Land-cover data were provided by the 2001 and 2006 National Land Cover Database: the 2001 classification was used from 1994 through 2003, and the 2006 classification was used from 2004 through 2010. Soil data used in the model included hydrologic soils group and the available soil-water capacity. These soil data were obtained from the Natural Resources Conservation Service Soil Survey Geographic (SSURGO) database and the State Soil Geographic (STATSGO) database. The statewide mean annual potential recharge rate from 1996&ndash;2010 was 4.9 inches per year. Potential recharge estimates increased from west to east across Minnesota. The mean annual potential recharge estimates across Minnesota at a 1-km resolution for the overall simulation period (1996&ndash;2010) ranged from less than 0.1 to 17.8 inches per year. Some of the lowest potential recharge rates for the simulation period were in the Red River of the North Basin of northwestern Minnesota, and generally were between 1.0 and 1.5 inches per year. The highest potential recharge rates were in northeastern Minnesota and the Anoka Sand Plain in central Minnesota. Eighty-eight percent of the potential recharge rates (by grid cell) were between 2 and 8 inches per year from 1996&ndash;2010. Only about 3 percent of all the potential recharge estimates (by grid cell) were less than 2 inches per year, and 9 percent of estimates were greater than 8 inches per year. On an annual basis, however, potential recharge rates were as high as 27.2 inches per year. The highest annual mean recharge estimate across the State was for 2010, and the lowest mean recharge estimate was for 2003. Although precipitation variability partially explained the annual differences in potential recharge estimates, precipitation alone did not account for these differences, and other factors such as antecedent moisture conditions likely were important. Also, because precipitation gradients across the State can vary from year to year, the dominant land-cover class and hydrologic soil group combinations for a particular region had a large effect on the resulting potential recharge value. During 1996&ndash;2010, April had the greatest monthly mean potential recharge compared to all other months, accounting for a mean of 30 percent of annual potential recharge in this single month.

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Gray Wolf ( Canis lupus ) dyad monthly association rates by demographic group.

Preliminary data from GPS-collared wolves (Canis lupus) in the Superior National Forest of northeastern Minnesota indicated wolves had low association rates with packmates during summer. However, aerial-telemetry locations of very high frequency (VHF)-radioed wolves in this same area showed high associations among packmates during winter. We analyzed aerial-telemetry-location data from VHF-collared wolves in several packs (n=18 dyads) in this same area from 1994-2012 by month, and found lowest association rates occurred during June. While other studies have found low association among wolf packmates during summer, information on differences in association patterns depending on the wolf associates&rsquo; demographics is sparse. During May-July, association rates were greatest for breeding pairs, followed by sibling dyads, and lowest for parent&ndash; offspring dyads. Our findings improve our understanding of how individual wolf relationships affect monthly association rates. We highlight some important remaining questions regarding wolf packmate associations.

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Reaction modeling of drainage quality in the Duluth Complex, northern Minnesota, USA

Reaction modeling can be a valuable tool in predicting the long-term behavior of waste material if representative rate constants can be derived from long-term leaching tests or other approaches. Reaction modeling using the REACT program of the Geochemist’s Workbench was conducted to evaluate long-term drainage quality affected by disseminated Cu-Ni-(Co-)-PGM sulfide mineralization in the basal zone of the Duluth Complex where significant resources have been identified. Disseminated sulfide minerals, mostly pyrrhotite and Cu-Fe sulfides, are hosted by clinopyroxene-bearing troctolites. Carbonate minerals are scarce to non-existent. Long-term simulations of up to 20 years of weathering of tailings used two different sets of rate constants: one based on published laboratory single-mineral dissolution experiments, and one based on leaching experiments using bulk material from the Duluth Complex conducted by the Minnesota Department of Natural Resources (MNDNR). The simulations included only plagioclase, olivine, clinopyroxene, pyrrhotite, and water as starting phases. Dissolved oxygen concentrations were assumed to be in equilibrium with atmospheric oxygen. The simulations based on the published single-mineral rate constants predicted that pyrrhotite would be effectively exhausted in less than two years and pH would rise accordingly. In contrast, only 20 percent of the pyrrhotite was depleted after two years using the MNDNR rate constants. Predicted pyrrhotite depletion by the simulation based on the MNDNR rate constant matched well with published results of laboratory tests on tailings. Modeling long-term weathering of mine wastes also can provide important insights into secondary reactions that may influence the permeability of tailings and thereby affect weathering behavior. Both models predicted the precipitation of a variety of secondary phases including goethite, gibbsite, and clay (nontronite).

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Copper toxicity and organic matter: Resiliency of watersheds in the Duluth Complex, Minnesota, USA

We estimated copper (Cu) toxicity in surface water with high dissolved organic matter (DOM) for unmined mineralized watersheds of the Duluth Complex using the Biotic Ligand Model (BLM), which evaluates the effect of DOM, cation competition for biologic binding sites, and metal speciation. A sediment-based BLM was used to estimate stream-sediment toxicity; this approach factors in the cumulative effects of multiple metals, incorporation of metals into less bioavailable sulfides, and complexation of metals with organic carbon. For surface water, the formation of Cu-DOM complexes significantly reduces the amount of Cu available to aquatic organisms. The protective effects of cations, such as calcium (Ca) and magnesium (Mg), competing with Cu to complex with the biotic ligand is likely not as important as DOM in water with high DOM and low hardness. Standard hardness-based water quality criteria (WQC) are probably inadequate for describing Cu toxicity in such waters and a BLM approach may yield more accurate results. Nevertheless, assumptions about relative proportions of humic acid (HA) and fulvic acid (FA) in DOM significantly influence BLM results; the higher the HA fraction, the higher calculated resiliency of the water to Cu toxicity. Another important factor is seasonal variation in water chemistry, with greater resiliency to Cu toxicity during low flow compared to high flow. Based on generally low total organic carbon and sulfur content, and equivalent metal ratios from total and weak partial extractions, much of the total metal concentration in clastic streambedsediments may be in bioavailable forms, sorbed on clays or hydroxide phases. However, organicrich fine-grained sediment in the numerous wetlands may sequester significant amount of metals, limiting their bioavailability. A high proportion of organic matter in waters and some sediments will play a key role in the resiliency of these watersheds to potential additional metal loads associated with future mining operations.

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Toxicity of smelter slag-contaminated sediments from Upper Lake Roosevelt and associated metals to early life stage White Sturgeon (Acipenser transmontanus Richardson, 1836)

The toxicity of five smelter slag-contaminated sediments from the upper Columbia River and metals associated with those slags (cadmium, copper, zinc) was evaluated in 96-h exposures of White Sturgeon ( Acipenser transmontanus Richardson, 1836) at 8 and 30 days post-hatch. Leachates prepared from slag-contaminated sediments were evaluated for toxicity. Leachates yielded a maximum aqueous copper concentration of 11.8 &mu;g L &minus;1 observed in sediment collected at Dead Man's Eddy (DME), the sampling site nearest the smelter. All leachates were nonlethal to sturgeon that were 8 day post-hatch (dph), but leachates from three of the five sediments were toxic to fish that were 30 dph, suggesting that the latter life stage is highly vulnerable to metals exposure. Fish maintained consistent and prolonged contact with sediments and did not avoid contaminated sediments when provided a choice between contaminated and uncontaminated sediments. White Sturgeon also failed to avoid aqueous copper (1.5&ndash;20 &mu;g L &minus;1 ). In water-only 96-h exposures of 35 dph sturgeon with the three metals, similar toxicity was observed during exposure to water spiked with copper alone and in combination with cadmium and zinc. Cadmium ranging from 3.2 to 41 &mu;g L &minus;1 or zinc ranging from 21 to 275 &mu;g L &minus;1 was not lethal, but induced adverse behavioral changes including a loss of equilibrium. These results suggest that metals associated with smelter slags may pose an increased exposure risk to early life stage sturgeon if fish occupy areas contaminated by slags.

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Stream sediment sources in midwest agricultural basins with land retirement along channel

Documenting the effects of agricultural land retirement on stream-sediment sources is critical to identifying management practices that improve water quality and aquatic habitat. Particularly difficult to quantify are the effects from conservation easements that commonly are discontinuous along channelized streams and ditches throughout the agricultural midwestern United States. Our hypotheses were that sediment from cropland, retired land, stream banks, and roads would be discernible using isotopic and elemental concentrations and that source contributions would vary with land retirement distribution along tributaries of West Fork Beaver Creek in Minnesota. Channel-bed and suspended sediment were sampled at nine locations and compared with local source samples by using linear discriminant analysis and a four-source mixing model that evaluated seven tracers: In, P, total C, Be, Tl, Th, and Ti. The proportion of sediment sources differed significantly between suspended and channel-bed sediment. Retired land contributed to channel-bed sediment but was not discernible as a source of suspended sediment, suggesting that retired-land material was not mobilized during high-flow conditions. Stream banks were a large contributor to suspended sediment; however, the percentage of stream-bank sediment in the channel bed was lower in basins with more continuous retired land along the riparian corridor. Cropland sediments had the highest P concentrations; basins with the highest cropland-sediment contributions also had the highest P concentrations. Along stream reaches with retired land, there was a lower proportion of cropland material in suspended sediment relative to sites that had almost no land retirement, indicating less movement of nutrients and sediment from cropland to the channel as a result of land retirement.

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Re-evaluating the northeastern Minnesota moose decline and the role of wolves

We re-evaluated findings from Lenarz et al. (2009) that adult moose ( Alces alces ) survival in northeastern Minnesota was related to high January temperatures and that predation by wolves ( Canis lupus ) played a minor role. We found significant inverse relationships between annual wolf numbers in part of the moose range and various moose demographics from 2003 to 2013 that suggested a stronger role of wolves than heretofore believed. To re-evaluate the temperature findings, we conducted a simulation study, mimicking the approach taken by Lenarz et al. (2009), to explore the potential for concluding a significant relationship exists between temperature and survival, when no association exists. We found that the high R 2 s and low probabilities associated with the regression models in Lenarz et al. (2009) should be viewed cautiously in light of the large number of fitted models (m = 45) and few observations (n = 6 for each of 5 response variables).

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How hot is too hot? Live-trapped gray wolf rectal temperatures and 1-year survival

The ability of physically restrained and anesthetized wolves to thermoregulate is lessened and could lead to reduced survival, yet no information is available about this subject. Therefore, we analyzed rectal temperatures related to survival 1 year post-capture from 173 adult (non-pup) gray wolves ( Canis lupus ) captured in modified foot-hold traps for radiocollaring during June&ndash;August, 1988&ndash;2011, in the Superior National Forest of northeastern Minnesota, USA. The maximum observed rectal temperature (&ldquo;maxtemp,&rdquo; &deg; F, &deg; C) in each wolf during capture ( x &thinsp;=&thinsp;104.0, 40.0; SD&thinsp;=&thinsp;2.0, 1.1; min.&thinsp;=&thinsp;95.9, 35.5; max.&thinsp;=&thinsp;108, 42.2) was not a significant predictor of survival to 1 year post-capture. Although no weather or morphometric variable was a significant predictor of maxtemps, wolves initially anesthetized with ketamine&ndash;xylazine rather than telazol&reg;&ndash;xylazine averaged higher maxtemps. This information does not fully address possible effects of high body temperatures related to live-capture and handling of wolves, but it does provide a useful waypoint for future assessments of this relationship and a reassurance to wildlife practitioners that the maxtemps observed in our study did not appear to affect 1-year survival.

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Trophic state in Voyageurs National Park lakes before and after implementation of a revised water-level management plan

We compiled Secchi depth, total phosphorus, and chlorophyll a (Chla) data from Voyageurs National Park lakes and compared datasets before and after a new water-level management plan was implemented in January 2000. Average Secchi depth transparency improved (from 1.9 to 2.1 m, p = 0.020) between 1977-1999 and 2000-2011 in Kabetogama Lake for August samples only and remained unchanged in Rainy, Namakan, and Sand Point Lakes, and Black Bay in Rainy Lake. Average open-water season Chla concentration decreased in Black Bay (from an average of 13 to 6.0 &mu;g/l, p = 0.001) and Kabetogama Lake (from 9.9 to 6.2 &mu;g/l, p = 0.006) between 1977-1999 and 2000-2011. Trophic state index decreased significantly in Black Bay from 59 to 51 (p = 0.006) and in Kabetogama Lake from 57 to 50 (p = 0.006) between 1977-1999 and 2000-2011. Trophic state indices based on Chla indicated that after 2000, Sand Point, Namakan, and Rainy Lakes remained oligotrophic, whereas eutrophication has decreased in Kabetogama Lake and Black Bay. Although nutrient inputs from inflows and internal sources are still sufficient to produce annual cyanobacterial blooms and may inhibit designated water uses, trophic state has decreased for Kabetogama Lake and Black Bay and there has been no decline in lake ecosystem health since the implementation of the revised water-level management plan.

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Identifying non-point sources of endocrine active compounds and their biological impacts in freshwater lakes

Contaminants of emerging concern, particularly endocrine active compounds (EACs), have been identified as a threat to aquatic wildlife. However, little is known about the impact of EACs on lakes through groundwater from onsite wastewater treatment systems (OWTS). This study aims to identify specific contributions of OWTS to Sullivan Lake, Minnesota, USA. Lake hydrology, water chemistry, caged bluegill sunfish ( Lepomis macrochirus ), and larval fathead minnow ( Pimephales promelas ) exposures were used to assess whether EACs entered the lake through OWTS inflow and the resultant biological impact on fish. Study areas included two OWTS-influenced near-shore sites with native bluegill spawning habitats and two in-lake control sites without nearby EAC sources. Caged bluegill sunfish were analyzed for plasma vitellogenin concentrations, organosomatic indices, and histological pathologies. Surface and porewater was collected from each site and analyzed for EACs. Porewater was also collected for laboratory exposure of larval fathead minnow, before analysis of predator escape performance and gene expression profiles. Chemical analysis showed EACs present at low concentrations at each study site, whereas discrete variations were reported between sites and between summer and fall samplings. Body condition index and liver vacuolization of sunfish were found to differ among study sites as did gene expression in exposed larval fathead minnows. Interestingly, biological exposure data and water chemistry did not match. Therefore, although results highlight the potential impacts of seepage from OWTS, further investigation of mixture effects and life history factor as well as chemical fate is warranted.

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Fishing for Northern Pike in Minnesota: A comparison of anglers and dark house spearers

In order to project fishing effort and demand of individuals targeting Northern Pike Esox lucius in Minnesota, it is important to understand the catch orientations, management preferences, and site choice preferences of those individuals. Northern Pike are specifically targeted by about 35% of the approximately 1.5 million licensed anglers in Minnesota and by approximately 14,000–15,000 dark house spearers. Dark house spearing is a traditional method of harvesting fish through the ice in winter. Mail surveys were distributed to three research strata: anglers targeting Northern Pike, dark house spearing license holders spearing Northern Pike, and dark house spearing license holders angling for Northern Pike. Dark house spearers, whether spearing or angling, reported a stronger orientation toward keeping Northern Pike than did anglers. Anglers reported a stronger orientation toward catching large Northern Pike than did dark house spearers when spearing or angling. Northern Pike regulations were the most important attribute affecting site choice for respondents in all three strata. Models for all strata indicated a preference for lakes without protected slot limits. However, protected slot limits had a stronger negative influence on lake preference for dark house spearing licensees (whether spearing or angling) than for anglers.

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Lacustrine responses to decreasing wet mercury deposition rates: results from a case study in northern Minnesota

We present a case study comparing metrics of methylmercury (MeHg) contamination for four undeveloped lakes in Voyageurs National Park to wet atmospheric deposition of mercury (Hg), sulfate (SO 4 –2 ), and hydrogen ion (H + ) in northern Minnesota. Annual wet Hg, SO 4 –2 , and H + deposition rates at two nearby precipitation monitoring sites indicate considerable decreases from 1998 to 2012 (mean decreases of 32, 48, and 66%, respectively). Consistent with decreases in the atmospheric pollutants, epilimnetic aqueous methylmercury (MeHg aq ) and mercury in small yellow perch (Hg fish ) decreased in two of four lakes (mean decreases of 46.5% and 34.5%, respectively, between 2001 and 2012). Counter to decreases in the atmospheric pollutants, MeHg aq increased by 85% in a third lake, whereas Hg fish increased by 80%. The fourth lake had two disturbances in its watershed during the study period (forest fire; changes in shoreline inundation due to beaver activity); this lake lacked overall trends in MeHg aq and Hg fish . The diverging responses among the study lakes exemplify the complexity of ecosystem responses to decreased loads of atmospheric pollutants.

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