Geology topics
P. E. McAllister
Publications and source records attributed to P. E. McAllister.
Performance of nine external tags on hatchery-reared rainbow trout
We evaluated nine commercially available tags to determine their suitability for marking yearling rainbow trout Oncorhynchus mykiss reared in raceways and circular tanks. The tags tested were Floy vinyl tubing tags FD‐67 anchor, FT‐2 dart, FT‐4 cinch‐up, and FT‐4 lock‐on; modified Carlin; modified Petersen disk; Monel strap 4‐1005; Monel butt end 4‐1242; and Stoffel fish seal. After 9 months, tag losses from raceway fish were lowest (8%) for the FT‐4 lock‐on tag; in tanks, none of the FT‐2 dart, FT‐4 cinch‐up, FT‐4 lock‐on, or the Monel butt end tags were lost. At 90 d after tagging, raceway fish marked with the Monel strap or the modified Carlin tags showed significantly less growth than did fish marked with the FD‐67 anchor tag; the converse was true for fish in tanks. After 9 months, raceway fish marked with the Monel butt end tag and tank fish marked with the FD‐67 anchor tag were least injured. Injury was greater in raceways than in tanks for all tag types. Judged by overall performance, the FD‐67 anchor was the most effective tag for fish maintained in raceways and the FT‐4 cinch‐up was a suitable alternative; the FT‐2 dart, FT‐4 cinch‐up, and Monel butt end tags were equally suitable for fish in tank culture.
Treatment of fish eggs by infection of chemicals into the incubator water supply
A system for injecting chemicals into a water supply to treat fish or fish eggs is described. Equations for calculating treatment mixtures and a sample computation are given.
Transmission of infectious pancreatic necrosis virus from virus carrier striped bass to brook trout
No abstract available.
Monoclonal antibody produced against viral hemorrhagic septicemia virus
Explore the source record for details and available documents.
Detection of infectious pancreatic necrosis virus in pelleted cell and particulate components from ovarian fluid of brook trout Salvelinus fontinalis
No abstract available.
Identification of the three serotypes of viral hemorrhagic septicemia virus by immunoblot assay using antiserum to serotype F1
Explore the source record for details and available documents.
Detection of infectious pancreatic necrosis virus can be enhanced by fractionating ovarian fluid
No abstract available.
Effect of exogenous corticosteroids on circulating virus and neutralizing antibodies in striped bass (Morone saxatilis) infected with infectious pancreatic necrosis virus
Explore the source record for details and available documents.
Infectious pancreatic necrosis virus: Protocol for a standard challenge to brook trout
A protocol for experimental challenge with infectious pancreatic necrosis (IPN) virus was defined with brook trout Salvelinus fontinalis as the model species. Fish were exposed by immersion for 5 h in water containing IPN virus at a concentration of 10 5 plaque‐forming units per milliliter. We propose the protocol as a standard challenge based on our studies of exposure methods and host response to virulent virus. Immersion challenge induced higher and more consistent mortality than did challenge by hyperosmotic infiltration. Challenge virus should be sequentially transferred no more than five times in cell culture because further transfers reduced the virulence of the virus. At 12°C, mortalities due to primary infection occurred 6–12 d after immersion challenge and were highest (≥70%) in fish 27–56 d old. Susceptibility to lethal infection was enhanced by nutritional stress.
Infectious pancreatic necrosis virus in striped bass Morone saxatilis: experimental infection of fry and fingerlings
No abstract available.
Immunoblot assay: a rapid and sensitive method for identification of salmonid fish viruses
An immunoblot assay was used to identify the viruses of infectious pancreatic necrosis, infectious hematopoietic necrosis, and viral hemorrhagic septicemia. Viral antigen in infected cell culture supernatant was adsorbed onto nitrocellulose membrane or Whatman 541 filter paper and detected by enzyme-linked immunosorbent assay techniques. The immunoblot assay took less than 4 hr to perform and required no special instrumentation. Assays using cell culture supernatant fluids showed immunoblot sensitivity was 10 5 –10 6 PFU/ml. Assay sensitivity, determined using purified virus, is 0.85–4.0 ng of viral antigen. The immunoblot assay was used to detect and identify virus in cell culture fluids.
Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus
A hybridoma cell line that continually secretes antibody against infectious hematopoietic necrosis virus was developed and designated M-IHNV-W1. Antibodies in culture fluids were concentrated and purified by Protein A Sepharose CL-4B affinity chromatography. The immunoglobulin was isotyped as IgG 2b with a kappa light chain. The IgG was specific for infectious hematopoietic necrosis virus and had binding activity but low neutralizing activity. Little cross-reactivity occurred with viral hemorrhagic septicemia or infectious pancreatic necrosis viruses.
Viral diseases of fish: first report of carp pox in golden ide (Leuciscus idus) in North America
Carp pox, a putative viral disease exotic to North America, occurred in golden ide 1 yr after the fish were imported into the United States from the Federal Republic of Germany. The raised, white, plaque-like lesions, which occurred on about 5% of the fish, healed spontaneously and caused no mortality. Electron micrographs showed herpesvirus-like particles associated with lesion specimens; however, no infectious viruses were detected in tests with seven warmwater fish cell lines.
Isolation of infectious pancreatic necrosis virus from estuarine fish
Explore the source record for details and available documents.