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Regreening, restoring, and reconnecting a southwestern wetland ecosystem – the Zeedyk wetland

Alluvial wetland ecosystems are vital as biodiversity hotspots but are increasingly threatened by anthropogenic stressors and drought. These pressures are especially acute in arid and semi-arid regions, where eco-hydrologic connectivity is fragile and recovery is slow. This study quantifies the efficacy of nature-based solutions, particularly the ‘Zeedyk approach,’ which employs low-tech Natural Infrastructure in Dryland Streams (NIDS)—including rock detention structures—to slow surface water, raise groundwater tables, and restore wetland function at a spring-fed wetland in Cebolla Canyon, New Mexico, U.S.A. Our results depict a Restoration Feedback Loop that captures stages of change from a healthy wetland in 1935, altered by 20th-century agriculture and grazing, to the re-establishment of the historical flow regime by 2024 documented through an 89-year archive of aerial imagery (1935–2024). By the end of our study period, the Spring-Fed Wetland had expanded by roughly 229% of the original 1935 area, to 4.13 ha. Using 40 years of satellite data, we assess changes in vegetation and hydrology with remote sensing indices. Spatial and temporal analyses reveal significant increases in vegetation greenness and wetness, particularly in an Expanded Wetland subregion, which exhibited ∼3.5x higher wetness and ∼1.5x higher greenness trends compared to adjacent areas. Monthly metrics highlight seasonal variability, with increases in greenness linked to monsoonal rainfall and lateral water redistribution, indicating that restoration impacts extend beyond the primary wetland. This study demonstrates the utility of cloud-based platforms like Google Earth Engine and USGS EarthExplorer for long-term monitoring of wetland restoration, while quantifying the efficacy of the ‘Zeedyk approach’ and demonstrating its potential as a scalable method to restore and conserve wetland meadows in other arid and semi-arid landscapes.

New Mexico

Wetlands, groundwater and seasonality influence the spatial distribution of stream chemistry in a low-relief catchment

Evaluating stream water chemistry patterns provides insight into catchment ecosystem and hydrologic processes. Spatially distributed patterns and controls of stream solutes are well-established for high-relief catchments where solute flow paths align with surface topography. However, the controls on solute patterns are poorly constrained for low-relief catchments where hydrogeologic heterogeneities and river corridor features, like wetlands, may influence water and solute transport. Here, we provide a data set of solute patterns from 58 synoptic surveys across 28 sites and over 32 months in a low-relief wetland-rich catchment to determine the major surface and subsurface controls along with wetland influence across the catchment. In this low-relief catchment, the expected wetland storage, processing, and transport of solutes is only apparent in solute patterns of the smallest subcatchments. Meanwhile, downstream seasonal and wetland influence on observed chemistry can be masked by large groundwater contributions to the main stream channel. These findings highlight the importance of incorporating variable groundwater contributions into catchment-scale studies for low-relief catchments, and that understanding the overall influence of wetlands on stream chemistry requires sampling across various spatial and temporal scales. Therefore, in low-relief wetland-rich catchments, given the mosaic of above and below ground controls on stream solutes, modeling efforts may need to include both surface and subsurface hydrological data and processes.

Michigan

Water table rise sustains carbon release from soils in wetland-dominated landscapes: An intact soil core study

Wetland-dominated landscapes influence carbon cycling through their potential to act as both carbon sinks and sources. Wetlands in low-relief landscapes have dynamic terrestrial-aquatic interfaces that change seasonally with variable surface water and groundwater levels. However, few studies have directly quantified dissolved organic matter (DOM) release and greenhouse gas (CO 2 , CH 4 ) fluxes from wetland soils along terrestrial-aquatic interfaces as they are seasonally re-saturated by groundwater. To estimate groundwater-mediated soil DOM and gas fluxes, we performed laboratory simulations of vertical groundwater rise on intact soil cores collected from four Delmarva bay wetlands located in the Mid-Atlantic United States. At each wetland, one core was collected from within the wetland basin and the other from the transitional zone at the basin edge. Cores were re-saturated with groundwater over 15 days and then kept fully saturated for an additional 25 days. Source groundwater, soil porewater, and exfiltrated surface water samples were collected and analyzed for pH, ORP, DOM concentration, and DOM optical indices. In both the wetland and transition zone cores, porewater DOM concentrations increased over the wet up and were sustained during prolonged saturation. Optical indices shifted from recently produced, microbial-like signatures towards aromatic, terrestrial-like signatures. Fluxes of CO 2 decreased as the duration of soil saturation increased and soil cores switched from CH 4 sinks to sources upon full soil core saturation. Results indicate that groundwater rise sustains carbon mobilization from soils in wetland-dominated landscapes, emphasizing the need to understand how climate-driven changes to groundwater dynamics may affect carbon fluxes along terrestrial-aquatic interfaces.

Delaware, Maryland

Temperate wetlands lose climate-cooling capacity under warming

Temperate inland wetlands have long been viewed as climate regulators, with sustained CO₂ sequestration offsetting continuous CH₄ emissions over centennial–millennial timescales. However, the net climatic effect of these opposing fluxes remains uncertain under anthropogenic warming as they differ in both magnitude and atmospheric lifetime. We combine 19,000 CH₄ chamber measurements from 202 temperate inland wetlands with carbon burial rates from 16 dated sediment cores in North America’s Prairie Pothole Region to estimate greenhouse gas dynamics over two centuries. Here we show that CH₄ emissions rise by ~1.39 kg CH₄ ha⁻¹ yr⁻¹ over the historical period and are projected to approach 950 kg CH₄ ha⁻¹ yr⁻¹ under high-forcing scenarios by 2100, while CO₂ sequestration is expected to decline by ~0.3 kg CO₂ ha⁻¹ yr⁻¹, driving a shift toward persistent net CO₂-equivalent source behaviour and a weakening of the long-term cooling effect that wetlands have provided under natural conditions.

Prairie Pothole Region

Towards a refined definition of “tidal influence” for the coastal wetland sciences

The astronomical tide relates to lunar (and to a lesser extent solar) influences on rising and falling water bodies, and is a key control of many coastal processes. However, tides manifest along coastal margins in different ways to muddle application beyond Sir Isaac Newton’s first descriptions. Here, we argue that tide, or specifically “tidal,” has not been adequately defined for the coastal wetland sciences to facilitate contemporary interdisciplinary distinction for rapidly changing coastlines and future climate adaptation planning. We provide a brief history of the tidal concept, and we describe tides in a diversity of uncommonly recognized marginal coastal environments. Common astronomical tide manifestations are described through hydrographic examples, then expanded to include those tidal environments exposed additionally to fluvial, meteorological (wind), or groundwater influences. Our expanded definition of “tidal” is intended to be a forward-looking construct as environments will inevitably change with sea-level rise, water extraction, and anthropogenic flow alteration. Our definition of wetland tidal influence, which we broaden, includes those “wetlands affected by astronomic water level fluctuations within coastally restricted environments causing frequent or infrequent surface inundation or groundwater tidal variation that affects the biogeochemistry of the soil (e.g., ion exchange capacity, oxygen state, mineralization processes)”. Astronomic water level fluctuations are often amplified or dampened by non-astronomical events, such as wind. Expanding the definition of what is considered tidal by the scientific community is critical for improved understanding of coastal wetland function and the suite of ecosystem services they provide people and society, including climate mitigation opportunity, management, and adaptation.

Wetlands

Hydrological control on soil redox condition and carbon loss of coastal wetland under sea-kevel rise

Coastal wetlands are critical carbon sinks with their biogeochemical and ecological functioning shaped by dynamic hydrological conditions that are increasingly influenced by climate change. A key unresolved question is how hydrologic flow, vegetation response, and rising sea levels interact to regulate soil redox condition and carbon loss in coastal wetlands. Using a field-tested hydrological–biogeochemical–ecological modeling framework, we reveal how the interplay between terrestrial groundwater discharge and tidal fluctuations generates complex groundwater flow patterns at the terrestrial–aquatic interface, and how these patterns modulate soil redox conditions, in turn influencing soil organic matter decomposition and carbon loss. Notably, rising sea levels suppress soil CO 2 emissions while reducing lateral dissolved carbon losses, thereby enhancing litter carbon sequestration under anoxic conditions. As vegetation responds to sea-level rise and carbon inputs diminish, litter carbon subsequently declines. These findings underscore a critical hydrological control on carbon cycling, advancing our understanding of coastal ecosystem resilience in a warming world.

Louisiana

Land cover change within wetland complexes at Dixie Meadows, Churchill County, Nevada: 2015 – 2023

Dixie Meadows, Nevada, is a system of geothermal springs and seeps that feed a complex of marshes and wetland meadows that are located within lands managed by the Bureau of Land Management (BLM) and the Department of Defense (DOD). A previous U.S. Geological Survey report documented variability in satellite imagery-based land cover classifications for seven wetland complexes at near monthly time intervals between October 2015 and January 2022. This report presents additional data, extending analysis to November 2023. Land cover classifications between October 2015 and November 2023 demonstrated an association between vegetation cover characteristics and surface moisture, with Class 1 having dry, bare soil or sparse upland vegetation, Class 2 having moist, bare soil or sparse to small vegetation, Class 3 having dense green vegetation with potentially saturated soil conditions, Class 4 having a mix of shallow surface water, saturated soil, and dense green vegetation, and Class 5 having open surface water. Most of the wetland complexes occur close to spring outflows primarily within land managed by the DOD, though portions are also within BLM lands. The intervening and surrounding landscape outside of the wetland complexes assessed in this study are managed by the BLM. As a result, Class 1 land covers had the largest areal coverage for BLM managed lands. Classes 2 and 3 land covers were primarily mapped inside the wetland complexes and thus had the largest area coverage within DOD managed lands. Class 4 was almost exclusively mapped within the wetland complexes and thus was largely contained within DOD managed lands. Class 5 (open water) was exclusively mapped in and adjacent to a single wetland complex with catchment ponds on land managed by the BLM. The distribution of these land cover classes over the study period was seasonally and annually variable. Land cover areas of Classes 1 and 2 were larger during the spring months. Conversely, land cover areas of Classes 3 and 4 tended to be greatest during the summer or fall. These patterns might be influenced by differences in seasonal water sources and phenology.

Nevada

Beyond the mangroves: A global synthesis of tidal forested wetland types, drivers and future information opportunities

There is increasing awareness of the global diversity of tidal forested wetlands (TFWs) and their significance in the provision of ecosystem services. These ecosystems, including mangrove forests, tidal freshwater forested wetlands, supratidal forests and transitional forests together span multiple climatic zones, geomorphic settings, and inundation and salinity regimes. We utilise case studies across five continents to demonstrate the state of knowledge among TFWs. Intertidal mangroves are the best-defined of the TFWs thanks to decades of research on their geomorphology, hydrology and ecology across their broad distribution. Non-mangrove forest settings, however, demonstrate more diverse hydrological, biochemical and vegetation conditions. In many cases, non-mangrove forests are situated at upper intertidal or supratidal elevations, where surface waters and groundwater are subject to interactions between tides freshwater inputs. Salinity datasets show variations ranging from tidal freshwater forested wetlands and ‘low-salinity mangroves’ to mesohaline or marine salinities, often with high temporal variability. While the floristic composition of non-mangrove forests vary among biogeographic regions, locally dominant TFW species are commonly distributed beyond the tidal niche into non-tidal wetland and upland forests. This presents challenges for traditional remote sensing approaches to ecosystem mapping, which are mostly lacking for non-mangrove forests. Geomorphic approaches and developments in machine learning offer opportunities to address this.

Preprint

Permafrost thaw controls iron flux from wetlands and sulfide-bearing rocks to Arctic rivers and streams

Recent warming has caused widespread iron mobilization into Arctic waterbodies that degrades ecosystems and threatens natural resources. Yet, understanding where and when iron flux occurs remains limited. Here, we investigate iron loading across regional to local scales in Arctic Alaska using climate, water chemistry, and borehole data together with mapped geology and permafrost presence. We show that both anoxic microbial iron reduction and acid rock drainage from iron-sulfide oxidation mobilize iron. Iron influx is strongly associated with lowland wetlands, sulfide-rich upland bedrock, and near-surface permafrost. Acid rock drainage chemistry correlates very strongly with the depth of seasonal thaw above permafrost from the previous year, indicating a one-year lag. These findings clarify the spatial and temporal dynamics of Arctic river rusting, provide a mechanistic understanding of the phenomenon, and may allow anticipation of its occurrence and assessment of its implications for aquatic ecosystem health and subsistence resources under ongoing climate change.

Alaska

End-user needs for remote sensing wetlands of the Prairie Pothole Region of North America

The Prairie Pothole Region (PPR) of North America comprises globally important grassland and wetland ecosystems critical for numerous populations of migratory birds. Due to the importance of this region for migratory birds, and particularly waterfowl, and the threats of habitat loss due to intensifying agriculture, there is a mature and diverse system of conservation organizations, agencies, and partnerships that spends hundreds of millions of dollars annually on habitat conservation to support migratory bird populations. Remote sensing can be a powerful tool for observing and evaluating global change at large scales as well as expanding inferences from field studies to the broader landscape with statistical models. However, development and utilization of these tools has lagged behind their demand for several reasons, including concerns over spatial and temporal resolution and accuracy of products; perception of a misalignment with decision-maker needs; technological barriers such as skill sets of conservation professionals, computing resources, data access, and usability. In this report, we summarize the needs of conservation professionals and scientists who use or want to use remote sensing data products to inform science about wetland change and conservation of wetlands in the PPR. We assembled this information through several methods leading up to, during, and following a January 2026 PPR Wetland Remote Sensing Workshop. The workshop included United States and Canadian scientists, conservation professionals, and policy experts. Our goal was to bring together end-users and remote sensing product developers jointly to explore reducing the lag between product development and utilization of products to inform science and conservation. Specifically, we aimed to identify gaps in wetland remote sensing that limit effective monitoring, management, and conservation in the PPR, and to develop a framework that outlines pathways to address these gaps by fostering collaboration, improving communication networks, encouraging discussion, and building on existing and ongoing efforts. This report summarizes our participants’ descriptions of end-user needs and the outcomes of the workshop.

Prairie Pothole region

From model to action: Identifying the gaps in coastal marsh models for decision making

Coastal salt marsh modeling projects are designed to answer questions about where salt marshes may migrate, how they can migrate, and how they will be impacted by changing water levels. While this information is vital for climate-resilient marsh conservation, translating these findings into actionable results remains a challenge. The authors examined the actionability of marsh modeling efforts in the United States by analyzing data collected from semi-structured interviews of marsh model users ( n = 24) across two ongoing projects along the U.S. East Coast and the Gulf of America (Gulf of Mexico). By qualitatively analyzing the interview data, the authors found that the tasks of practitioners who use coastal marsh model outputs fall into three major themes: (1) marsh restoration, (2) planning with uncertainty, and (3) conserving habitat for marsh-reliant species. For each of these themes, the authors identify unmet needs, including high spatial resolution information for local planning, accessible descriptions of uncertainty to increase user confidence, and the incorporation of human dimensions data (e.g., human alterations to the landscape such as impoundments and culverts) for a comprehensive understanding of the coastal salt marsh. Marsh modeling projects should strategize how to fulfil these unmet user needs so that marsh modeling outputs can better support decisions related to marsh conservation and restoration.

Gulf of America, United States East Coast

Irrigated agriculture influences selenium levels in an endangered marsh bird

Selenium bioaccumulation in aquatic food webs poses risks to wildlife, particularly in wetlands receiving irrigation runoff. The Salton Sea, California’s largest lake, is primarily sustained by agricultural drainage. This drainage creates wetland habitat along the lakeshore that many bird species depend on, including the federally endangered Yuma Ridgway’s rail ( Rallus obsoletus yumanensis ). However, these marshes may pose an ecological trap – attracting rails despite high selenium exposure. We captured rails during the 2020–2023 breeding seasons and compared rail selenium levels within three types of marshes (fed with irrigation runoff, Colorado River water, or groundwater). We collected blood, breast feathers, and head feathers of rails in all three water sources for selenium comparisons. We tagged adult rails with GPS transmitters to locate nests and foraging locations where we collected eggshells, unhatched eggs, and prey. We assessed selenium exposure by collecting multiple prey species commonly eaten by rails in all three water sources. Selenium concentrations varied among sampling locations. Selenium concentrations in most sample types were predominately influenced by water source and marsh inflow velocity (sometimes in combination with marsh size). Distance to inflow, however, did not influence selenium concentrations in any sample type. Selenium concentrations were highest in agricultural-fed marshes compared to river-fed and spring-fed marshes. Increased marsh inflow velocities resulted in lower selenium concentrations. Given the risk of an ecological trap, our results suggest that supplementing wetlands with Colorado River water could mitigate selenium bioaccumulation in Yuma Ridgway’s rails.

California

Wet meadow regeneration through restoration of biophysical feedbacks

Wet meadows are globally significant ecosystems that provide critical hydrological, ecological, and biogeochemical functions, yet their extent has declined dramatically due to land use changes and hydrologic alteration. These sedge-dominated wetlands exist at the drier end of the wetland gradient, maintained by shallow groundwater and periodic inundation. This paper is a global synthesis of the ecological, geomorphic, and hydrological dynamics of wet meadows, with an emphasis on alluvial systems, to inform effective restoration strategies. We compare wet meadows to other wetlands, classify them into palustrine, lacustrine, and alluvial types, then focus on alluvial wet meadows and discuss how their formation and persistence depend on ground and surface water interactions, sediment deposition and flow obstructions, all mediated by biological processes. In particular, we highlight the role of hydric graminoids in resisting erosion and maintaining soil cohesion, how beaver promote meadow persistence, and the significance of wet meadows as carbon sinks. We also present stratigraphic evidence demonstrating that incision, often triggered by anthropogenic activity or changing climate, is the primary mechanism of alluvial wet meadow degradation, resulting in water table decline and shifts in vegetation composition. Restoration requires reversing these incisional processes through techniques that elevate water tables, disperse flow and retain sediment—methods traditionally associated with either soil conservation or stream restoration. These include nature-based solutions that create obstructions such as beaver dams and their analogues, rock and wood-based obstructions and incision trench or gully filling and grading. Given their multifunctional value—including but not limited to flood attenuation, biodiversity support, and carbon sequestration—wet meadows warrant a focused restoration framework. This review advocates for a valley-floor scale restoration paradigm that integrates hydrological reconnection, sediment retention, and biological reinforcement to ensure long-term resilience of these systems in the face of changing climate and land use pressures.

Frontiers in Environmental Science

Water-resources inventory and assessment at Katahdin Woods and Waters National Monument

The U.S. Geological Survey, in cooperation with the National Park Service, prepared a water-resources inventory and assessment for Katahdin Woods and Waters National Monument (KAWW). This compilation includes published and publicly accessible hydrologic data and resource assessments of streams, rivers, ponds, lakes, wetlands, vernal pools, and groundwater in and near KAWW. It also includes reports and datasets summarizing attributes of KAWW’s hydrologic infrastructure, such as stream crossings, dams, wastewater discharge plants, groundwater monitoring wells, and U.S. Geological Survey streamflow-gaging stations. Descriptions of data and details of current limitations in available datasets are included. Wetland, groundwater, streamflow, and water-quality information are all limited. Hydrography data are available; however, there are limited ground-truth data. Accurate streamlines within KAWW were developed from light detection and ranging (lidar) as a part of this work. Hydrologic infrastructure information is available from multiple sources; however, differences exist among the datasets. Datasets are summarized in appendix 1.

Maine

Airborne geophysical analysis to decipher salinization for coastal Louisiana

Coastal Louisiana is known for saltwater intrusion that threatens wetlands, aquifers, and rivers. However, the extent of saltwater intrusion is not well understood. This study develops an innovative framework with airborne electromagnetic (AEM) data to map chloride concentration distributions for wetlands in the Mississippi River deltaic plain and Chenier plain as well as for the Mississippi River Valley alluvial aquifer (MRVA) and Chicot aquifer. Moreover, the framework maps chloride concentrations along the Mississippi River and Atchafalaya River. Key components in the framework include the establishment of resistivity-to-chloride concentration transformation, 3D resistivity architecture building through geostatistics, and the employment of a lithologic model. The transformation functions correlate AEM resistivity data with porewater salinity measurements and groundwater and river chloride samples. The results show that AEM data reliably infers soil water chloride concentrations and correlates well with the distribution of various marsh types. AEM data reveals extensive saltwater presence at depth and near the coast, originating from salt domes and the Gulf of Mexico, respectively. The saltwater upconing pattern in the Chicot aquifer is likely due to excessive groundwater withdrawals. The AEM data also confirms a distinct tongue of saltwater intruding into the Atchafalaya Basin from the Gulf. The AEM data helps to identify faults that are obscured or eroded at the surface, which appear as leaky barriers in the subsurface where dramatic changes in chloride concentration are apparent. Finally, this study uses the AEM data to infer the presence of an extensive seawater wedge in the Mississippi River and Atchafalaya River.

Louisiana

Quantifying methane emissions from a rich fen with uncrewed aircraft systems in boreal Alaska

Thawing of permafrost in northern latitudes is accelerating, potentially releasing substantial amounts of methane (CH 4 ) as forested permafrost plateaus transition into wetlands. This ecosystem shift alters the carbon exchange between the soil and atmosphere, influencing the permafrost-carbon feedback. Monitoring these changes may require measurement platforms operating across varied spatial and temporal scales. Recent advancements in small uncrewed aircraft systems (sUAS) enable high resolution CH 4 flux quantification in remote, complex terrains; however, comparisons with established methods such as eddy covariance flux towers remain limited. We used a hexacopter sUAS to quantify CH 4 emissions from the Alaska Peatland Experiment, a wetland within the Bonanza Creek Experimental Forest. Using an ensemble of methods to define the background CH 4 concentration, along with near surface emissions from soil chambers, helped constrain our flux estimates. The sUAS method yielded an average flux of 0.0077 ± 0.0019 mol s −1 CH 4 , within a factor of two concurrent tower-derived total source flux estimates (0.0036 ± 0.00042 mol s −1 CH 4 ). To assess spatial drivers of observed fluxes, we conducted a 2D footprint analysis and overlaid the results with high-resolution hyperspectral land cover classification, quantifying vegetative contributions within each footprint. This revealed higher fen representation in sUAS measurements (73.8%) than in tower footprints (58.8%), and lower tussock meadow representation (15.6% and 30.3%, respectively). These differences were consistent with known variation in vegetation-specific CH 4 emissions. Our results highlight that combining footprint modeling with land cover characterization can enhance interpretations of CH 4 fluxes and guide cross-platform comparisons.

JGR Atmospheres

Satellite time series analysis to quantify changing climax ciénegas using a state and transition model approach

Ciénegas are rare wetlands in arid landscapes of the North American Southwest, historically providing critical ecological and hydrological functions but increasingly threatened by changing climate and land use pressures. This study quantifies changes in ciénega condition and floodplain dynamics using a state-and-transition model (STM) informed by expert knowledge and remote sensing. Key factors include woody plant encroachment, water availability, and soil aggradation. We mapped 31 ciénegas with high-resolution imagery and analyzed Landsat data (1985–2023) to assess vegetation health and moisture using the Normalized Difference Vegetation Index (NDVI) and Normalized Difference Infrared Index (NDII). Results show substantial interannual variability in phenology, water stress, and soil moisture, with regional drying and elevation strongly influencing ciénega resilience. We classified ciénegas into three functional states—healthy, desiccated, and dormant—and mapped their 2023 condition. Trend analyses indicate most ciénegas exhibit greening despite drought, though localized variability underscores the need for site-specific management. None are in a stable climax (reference) state; rather, they transition among states in response to external drivers. Increasing woody plant cover and surface drying, likely linked to declining regional water tables, favor deep-rooted species over wetland grasses—a pattern mirrored in adjacent control plots. Spatially explicit analysis revealed intra-ciénega variability often masked by aggregated data, highlighting the importance of high-resolution monitoring. Seasonal and long-term trends provide context for understanding ciénega dynamics, including degradation and restoration pathways. This study emphasizes the importance of groundwater conservation and demonstrates how remote sensing supports long-term monitoring. The STM framework offers a practical tool for adaptive management to sustain freshwater resources in arid environments.

Arizona, New Mexico

Assessing environmental drivers of denitrification in restored riverine floodplains

Restoration of impaired floodplains is an increasingly prevalent strategy for alleviating water quality concerns and reducing downstream flooding at watershed scales. Floodplains temporarily store water and slow flow velocity to promote sedimentation during overbank flooding and remove inorganic nitrogen from floodwater and groundwater via denitrification. Evaluating the impacts of different restoration strategies on denitrification can inform more strategic investments into floodplain modifications that improve water quality outcomes. Our research investigates how denitrification rates in floodplains respond to environmental factors that are actionable from an engineering perspective through design and water resources management. We seasonally measured soil denitrification enzyme activity and various environmental characteristics in 4 floodplains with different restoration design and management approaches at the confluence of the Wabash and Tippecanoe Rivers in Indiana, United States. Our results showed that denitrification rates in an agricultural floodplain were significantly lower than in restored floodplains with native vegetation. Certain soil conditions characteristic of floodplain wetlands were associated with higher denitrification, particularly elevated total nitrogen, moisture, silt, and organic matter contents. Vegetation species composition was correlated with denitrification rates. This link may reflect the direct effects of vegetation on soil conditions, such as supplying labile organic carbon, or indirect effects, such as vegetation acting as an indicator of hydrologic regime and land use. Denitrification seasonally varied, peaking in winter when nitrate supply from rivers draining agricultural watersheds in the region is also high. Substrate limitation of soil denitrification enzyme activity was most significant during the summer when overbank flooding, which replenishes soil nitrogen stocks, rarely occurs. Our findings indicate that denitrification capacity will likely be maximized in riverine floodplains that are restored as wetlands with diverse native vegetation and enhanced hydrologic connectivity. Such restoration activities promote higher denitrification rates via elevated moisture, fine sediment deposition, and soil organic matter.

Tippecanoe River, Wabash River