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At least 19 recordsLinked to original sources

Evidence for the importance of invasive Dreissena veligers as a novel prey item for larval fish in Lake Huron

The establishment of invasive dreissenid mussels Dreissena polymorpha and Dreissena rostriformis bugensis in the Laurentian Great Lakes has affected multiple aspects of the ecosystem. However, the effects of their larvae (veligers) on lower trophic levels are relatively unknown. Previous research has documented that some larval fishes consume veligers, but it is unclear if they select for veligers. To assess the role of veligers in larval fish diets in Lake Huron, we examined the diets of larval burbot Lota lota , rainbow smelt Osmerus mordax , and Coregonus spp., mainly bloater Coregonus hoyi , sampled in July of 2017. Preference for available zooplankton prey was evaluated using Vanderploeg and Scavia’s E*. Results indicated that veligers were on average avoided by large larval burbot, rainbow smelt, and coregonines but were sometimes preferred by small (< 7 mm) and medium-sized (7–10 mm) larval burbot. A mixed model analyzing factors contributing to veliger preference by larval burbot indicated that greater environmental zooplankton prey size is associated with more positive preference for veligers. Thus, veligers may be important for gape-limited larval fish. We also found that, on average, larval burbot and coregonines consumed larger veligers than those sampled in the environment. Overall, consideration of larval fishes’ ability to exploit veligers could help managers to understand the role of dreissenid mussels in Great Lakes food webs.

Lake Huron↗

Planktivory by alewife ( Alosa pseudoharengus ) and rainbow smelt ( Osmerus mordax ) on microcrustacean zooplankton and dreissenid (Bivalvia: Dreissenidae) veligers in southern Lake Ontario

The objective of this study was to describe the diet of young-of-the-year and adult alewife ( Alosa pseudoharengus ) and rainbow smelt ( Osmerus mordax ) in nearshore waters coincident with the colonization of Lake Ontario by Dreissena . Laboratory experiments and field observations indicated that alewife and rainbow smelt consumed dreissenid veligers and that the veligers remained intact and identifiable in the digestive tract for several hours. Dreissenid larvae were found in field-caught alewife and rainbow smelt in August 1992, even though veliger densities were low (<0.1/L). Zooplankton dominated the diet of all fish and veliger larvae were <0.1% of the biomass of prey eaten by these fish. Density of veligers and the distribution of settled dreissenids declined from west to east along the south shore of Lake Ontario. Based on veliger consumption rates we measured and the abundance of veligers and planktivores, we conclude that planktivory by alewife and smelt in the nearshore waters of Lake Ontario did not substantially reduce the number of veligers during 1991&ndash;1993. However, our results indicate that if the density of veligers in Lake Ontario decreases, and if planktivores remain abundant, planktivory on veliger populations could be significant.

Canadian Journal of Fisheries and Aquatic Sciences↗

Bloom forming cyanobacteria can adversely affect zebra and quagga mussel veligers

Quagga ( Dreissena rostriformis bugensis ) and zebra ( D. polymorpha ) mussels are broadcast spawners that produce planktonic, free swimming veligers, a life history strategy dissimilar to native North American freshwater bivalves. Dreissenid veligers require highly nutritious food to grow and survive, and thus may be susceptible to increased mortality rates during harsh environmental conditions like cyanobacteria blooms. However, the impact of cyanobacteria and one of the toxins they can produce (microcystin) has not been evaluated in dreissenid veligers. Therefore, we exposed dreissenid veligers to eleven distinct cultures (isolates) of cyanobacteria representing Anabaena, Aphanizomenon, Dolichospermum, Microcystis, and Planktothrix species and the cyanotoxin microcystin to determine the lethality of cyanobacteria on dreissenid veligers. Six-day laboratory bioassays were performed in microplates using dreissenid veligers collected from the Detroit River, Michigan, USA. Veligers were exposed to increasing concentrations of cyanobacteria and microcystin using the green algae Chlorella minutissima as a control. Based on dose response curves formulated from a Probit model, the LC 50 values for cyanobacteria used in this study range between 15.06 and 135.06 μg/L chlorophyll- a , with the LC 50 for microcystin-LR at 13.03 μg/L. Because LC 50 values were within ranges observed in natural waterbodies, it is possible that dreissenid recruitment may be suppressed when veliger abundances overlap with seasonal cyanobacteria blooms. Thus, the toxicity of cyanobacteria to dreissenid veligers may be useful to include in models forecasting dreissenid mussel abundance and spread.

Ecotoxicology and Environmental Safety↗

Spatial and temporal distributions of Dreissena spp. veligers in Lake Huron: Does calcium limit settling success?

The larval stage of invasive Dreissena spp. mussels (i.e., veligers) are understudied despite their seasonal numerical dominance among plankton. We report the spring and summer veliger densities and size structure across the main basin, North Channel, and Georgian Bay of Lake Huron, and seek to explain spatiotemporal variation. Monthly sampling was conducted at 9 transects and up to 3 sites per transect from spring through summer 2017. Veliger densities peaked in June and July, and we found comparable densities and biomasses of veligers between basins, despite differences in density of juvenile and adult mussels across these regions. Using a generalized additive model to explain variations in veliger density, we found that temperature, chlorophyll a , and nitrates/nitrites were most important. We generated an index of veliger attrition based on size distributions that revealed a higher rate of attrition in the North Channel than the rest of the lake. A logistic model indicated a threshold calcium concentration of around 22 mg/L was necessary for veligers to survive to larger sizes and recruit to their juvenile and benthic adult life stages. Improved understanding of factors that regulate the production and survival of Dreissena veligers could improve the ability of managers to assess future invasion threats as well as explore potential control options.

Lake Huron↗

An experimental evaluation of the efficacy of imaging flow cytometry (FlowCam) for detecting invasive Dreissened and Corbiculid bivalve veligers

Zebra ( Dreissena polymorpha ) and quagga ( D. bugensis ) mussels, first introduced from central Asia into the Great Lakes of North America in the late 1980s, have crossed the continental divide and more recently spread across western North America. At the same time, several new technologies have been developed for the early detection of dreissenids, including the FlowCam, a digital imaging-in-flow instrument, intended to detect dreissenid planktonic larvae (veligers). However, the efficacy of this technology has rarely been tested. We experimentally evaluated the FlowCam’s ability to capture identifiable images of quagga mussel veligers under 2 different types of conditions: (i) deionized water, and (ii) Columbia River Basin water (CRBW), including natural sediment and native plankton. We further evaluated the FlowCam’s ability to distinguish between dreissenid veligers and corbiculid veligers (Asian clam, Corbicula fluminea ). We interpret our results to indicate that the FlowCam can consistently detect dreissenid veligers across a range of veliger densities. Moreover, the presence of other plankton and detritus only slightly affected dreissenid detection by the FlowCam. However, the orientation of individual bivalve veligers as they were imaged by the FlowCam precluded specific identification of a substantial proportion (24.8%) of veligers as either dreissenid or corbiculid. We suggest that the FlowCam is an important detection tool best utilized as part of a multifaceted approach, including traditional microscopy and possibly environmental DNA.

Lake and Reservoir Management↗

Mortality of zebra mussel, Dreissena polymorpha, veligers during downstream transport

1. Streams flowing from lakes which contain zebra mussels, Dreissena polymorpha , provide apparently suitable habitats for mussel colonization and downstream range expansion, yet most such streams contain few adult mussels. We postulated that mussel veligers experience high mortality during dispersal via downstream transport. They tested this hypothesis in Christiana Creek, a lake-outlet stream in south-western Michigan, U.S.A., in which adult mussel density declined exponentially with distance downstream. 2. A staining technique using neutral red was developed and tested to distinguish quickly live and dead veligers. Live and dead veligers were distinguishable after an exposure of fresh samples to 13.3 mg L −−1 of neutral red for 3 h. 3. Neutral red was used to determine the proportion of live veligers in samples taken longitudinally along Christiana Creek. The proportion of live veligers (mean ± SE) declined from 90 ± 3% at the lake outlet to 40 ± 8% 18 km downstream. 4. Veligers appear to be highly susceptible to damage by physical forces (e.g. shear), and therefore, mortality in turbulent streams could be an important mechanism limiting zebra mussel dispersal to downstream reaches. Predictions of zebra mussel spread and population growth should consider lake-stream linkages and high mortality in running waters.

Indiana, Michigan↗

Variations in zebra mussel ( Dreissena polymorpha ) veliger densities throughout 1996 at Dam 52 on the lower Ohio River

Zebra mussel veliger densities were monitored throughout 1996 at Lock and Dam 52 on the lower Ohio River near Brookport, IL. The spawning season occurred between mid June and early September with veliger densities peaking at 30,000/m 3 in late June. Veliger first appeared at a water temperature of 21&deg; C. When spawning ended in September the water temperature was 22 &deg;C. Veligers were found throughout November when the water temperature was 10 &deg;C. The lowest temperature at which veligers were observed was 7 &deg; C in March 1996. These results show that the zebra mussels in the lower Ohio River are reproducing naturally and that spawning appears to be synchronous. The presence of larvae at low temperatures in March suggests that veligers are capable of delaying settlement and overwintering in the planktonic stage.

Journal of Freshwater Ecology↗

Toxicity of a traditional molluscicide to asian clam veligers

Aquaculture and hatchery industries are in need of effective control methods to reduce the risk of spreading aquatic invasive species, such as the Asian clam Corbicula fluminea , through aquaculture and hatchery activities. The planktonic nature of Asian clam veligers enables this life stage to enter water-based infrastructure undetected, including hatchery trucks used to stock fish. Once in hatchery trucks, veligers can disperse overland and establish in previously uninvaded habitats. As a result, there is a need to develop techniques that result in veliger mortality but do not harm fish. In September 2012, we conducted laboratory trials to determine if a molluscicide (750 mg/L potassium chloride and 25 mg/L formalin) commonly used to kill zebra mussel ( Dreissena polymorpha ) veligers in hatchery trucks can also effectively kill Asian clam veligers. We exposed Asian clam veligers to this molluscicide for 1, 3, and 5 h in each of two water types: deionized water and filtered lake water. We found ,20% mortality at the 1-h exposure period and 100% mortality at both the 3-h and 5-h exposure periods, regardless of water type. This laboratory study represents an important step toward reducing the spread of Asian clams by aquaculture facilities.

Journal of Fish and Wildlife Management↗

Veligers of the invasive Asian clam Corbicula fluminea in the Columbia River Basin: Broadscale distribution, abundance, and ecological associations

The invasive Asian clam Corbicula fluminea was introduced to North America in the 1930s and now inhabits most regions of the conterminous United States; however, the distribution and ecology of C. fluminea in the Columbia River Basin is poorly understood. During 2013 and 2014, 5 Columbia-Snake River reservoirs were sampled monthly from May through September, along with 23 additional lakes and reservoirs sampled once each summer. Associations among C. fluminea veligers, other components of the plankton, and environmental variables were analyzed using non-metric multidimensional scaling and canonical correspondence analysis. Corbicula fluminea veligers were found in high abundances in all mainstem Columbia-Snake River reservoirs, with an annual mean abundance of 71.2 individuals per cubic meter (inds./m 3 ). Only 3 of 23 lakes and (non-mainstem) reservoirs contained C. fluminea , with abundances considerably lower (maximum = 21.2 inds./m 3 ) than in the mainstem reservoirs. A diatom-dominated community preceded the spawning of C. fluminea in early summer at all sites. Corbicula fluminea veligers characterized the plankton community in late summer and were associated with cyanobacteria and high water temperatures. A third community, characterized by cyanobacteria, was apparent in non-mainstem sites in July and August. Our analyses describe the relationship of C. fluminea to the plankton community and environment, which contributes to our understanding of the possible effects of C. fluminea infestations and which waterbodies in the Columbia River Basin are at risk for infestation. Understanding the effects and environmental determinants of invasive mollusks will be increasingly important in the future with the possible arrival of zebra ( Dreissena polymorpha ) or quagga ( D. bugensis ) mussels to the region.

Lake and Reservoir Management↗

Toxicity of potassium chloride to veliger and byssal stage dreissenid mussels related to water quality

Natural resource managers are seeking appropriate chemical eradication and control protocols for infestations of zebra mussels, Dreissena polymorpha (Pallas, 1769), and quagga mussels. D. rostiformis bugensis (Andrusov, 1897) that have limited effect on non-target species. Applications of low concentrations of potassium salt (as potash) have shown promise for use where the infestation and treatment can be contained or isolated. To further our understanding of such applications and obtain data that could support a pesticide registration, we conducted studies of the acute and chronic toxicity of potassium chloride to dreissenid mussels in four different water sources from infested and non-infested locations (ground water from northern Idaho, surface water from the Snake River, Idaho, USA, surface water from Lake Ontario, Ontario, Canada, and surface water from the Colorado River, Arizona, USA). We found short term exposure of veligers (< 24 h) to concentrations of 960 mg/L KCl produced rapid mortality in water from three locations, but veligers tested in Colorado River water were resistant. We used probit models to compare the mortality responses, predicted median lethal times and 95% confidence intervals. In separate experiments, we explored the sensitivity of byssal stage mussels in chronic exposures (>29 d) at concentrations of 100 and 200 mg/L KCl. Rapid mortality occurred within 10 d of exposure to concentrations of 200 mg/L KCl, regardless of water source. Kaplan-Meier estimates of mean survival of byssal mussels in 100 mg/L KCl prepared in surface water from Idaho and Lake Ontario were 4.9 or 6.9 d, respectively; however, mean survival of mussels tested in the Colorado River water was > 23 d. The sodium content of the Colorado River water was nearly three times that measured in waters from the other locations, and we hypothesized sodium concentrations may affect mussel survival. To test our hypothesis, we supplemented Snake River and Lake Ontario water with NaCl to equivalent conductivity as the Colorado River, and found mussel survival increased to levels observed in tests of veliger and byssal mussels in Colorado River water. We recommend KCl disinfection and eradication protocols must be developed to carefully consider the water quality characteristics of treatment locations.

Management of Biological Invasions↗

Spawning of zebra mussels (Dreissena polymorpha) and rearing of veligers under laboratory conditions

The spawning cycle of the zebra mussel, Dreissena polymorpha, is amenable to laboratory manipulations. Techniques are presented that can be used to initiate spawning and rear veligers from fertilized egg to settlement stage. Spawning can be induced in sexually mature mussels by temperature flucuations or by the addition of ripe gametes. Embryonic survival is excellent until the straight-hinge stage when the first wave of mortality occurs, usually due to improper food. The second critical stage of development occurs just prior to settlement when mortality increases again. Veliger mortality averaged over 90% from egg to settlement. The results indicate that obtaining large numbers of veligers for laboratory experiments to be conducted year-round is difficult.

Book chapter↗

Assessing a low-dose copper treatment for dreissenid mussels: Effects on zebra mussel (Dreissena polymorpha) population

We conducted and evaluated a low-dose copper treatment (applied as EarthTec QZ) to suppress zebra mussel ( Dreissena polymorpha Pallas 1771) veliger abundance and settlement in a 66.3 ha bay in Lake Minnetonka (Hennepin County, MN) over a 3-y period. We maintained a mean (standard deviation [SD]) concentration of 83.0 (10.3) µg/L as copper over the 10-d treatment period, much lower than the maximum allowable 1 mg/L as copper. Veliger density was reduced from 6.0 veligers/L before treatment to 0.3 veligers/L following the treatment period. Posttreatment zebra mussel settlement was 1900 times lower in the treated bay compared to an untreated bay days after the treatment despite similar pretreatment veliger densities. Veliger density and settlement remained suppressed nearly 2 y following the treatment. Sampling for adult zebra mussels within the treated bay returned variable results but survival of caged adult zebra mussels indicated ~30% treatment-related mortality. Copper in surface waters returned to near pretreatment concentrations 90 d after treatment. Our study demonstrates that low-dose applications of a copper molluscicide can effectively reduce zebra mussel veliger densities and settlement.

Minnesota↗

Procedures for conducting underwater searches for invasive mussels (Dreissena sp.)

Zebra mussels (Dreissena polymorpha) were first detected in the Great Lakes in 1988. They were likely transported as larvae or young adults inside the ballast tanks of large ocean-going ships originating from Europe. Since their introduction, they have spread throughout the Eastern, Midwestern, and Southern United States. In 2007, Quagga mussels (Dreissena rostriformis bugensis) were found in the Western United States in Lake Mead, Nevada; part of the Lower Colorado River Basin. State and Federal managers are concerned that the mussels (hereafter referred to as dreissenid mussels or mussels) will continue to spread to the Columbia River Basin and have a major impact on the region?s ecosystem, water delivery infrastructure, hydroelectric projects, and the economy. The transport and use of recreational watercraft throughout the Western United States could easily result in spreading mussels to the Columbia River Basin. The number of recreational watercraft using Lake Mead can range from 350 to 3,500 a day (Bryan Moore, National Park Service, oral commun., June 21, 2008). Because recreational watercrafts are readily moved around and mussels may survive for a period of time when they are out of the water, there is a high potential to spread mussels from Lake Mead to other waterways in the Western United States. Efforts are being made to prevent the spread of mussels; however, there is great concern that these efforts will not be 100 percent successful. When prevention efforts fail, early detection of mussels may provide an opportunity to implement rapid response management actions to minimize the impact. Control and eradication efforts are more likely to be successful if they are implemented when the density of mussels is low and the area of infestation is small. Once the population grows and becomes established, the mussels are extremely difficult, if not impossible, to control. Although chemicals may be used to kill the mussels, the chemicals that are currently available also can kill other aquatic life. Early implementation of containment and eradication efforts requires getting reliable information to confirm the location of the infestation. One way to get this information is through the use of properly trained SCUBA divers. This document provides SCUBA divers with the necessary information to conduct underwater searchers for mussels. However, using SCUBA divers to search for mussels over a large geographic area is relatively expensive and inefficient. Early detection monitoring methods can be used to optimize the use of SCUBA divers. Early detection monitoring can be accomplished by collecting water samples or deploying artificial settlement substrates (fig. 1). Water samples are used to look for free-swimming larval mussels (called veligers). Because the veligers cannot be identified with the naked eye, the water samples are sent to a laboratory where they are examined under a microscope and/or analyzed using molecular techniques to detect veligers. To detect the presences of adult mussels, artificial substrates are deployed and periodically retrieved to determine if mussels have settled on the substrate. If veligers or adults are identified, SCUBA divers can be deployed to confirm the presence of mussels.

Open-File Report↗

Spatio-temporal spawning and larval dynamics of a zebra mussel (Dreissena polymorpha) population in a North Texas Reservoir: implications for invasions in the southern United States

Zebra mussels were first observed in Texas in 2009 in a reservoir (Lake Texoma) on the Texas-Oklahoma border. In 2012, an established population was found in a near-by reservoir, Ray Roberts Lake, and in June 2013, settled mussels were detected in a third north Texas reservoir, Lake Lewisville. An established population was detected in Belton Lake in September 2013. With the exception of Louisiana, these occurrences in Texas mark the current southern extent of the range of this species in the United States. Previous studies indicate that zebra mussel populations could be affected by environmental conditions, especially increased temperatures and extreme droughts, which are characteristic of surface waters of the southern and southwestern United States. Data collected during the first three years (2010–12) of a long-term monitoring program were analyzed to determine if spatio-temporal zebra mussel spawning and larval dynamics were related to physicochemical water properties in Lake Texoma. Reproductive output of the local population was significantly related to water temperature and lake elevation. Estimated mean date of first spawn in Lake Texoma was approximately 1.5 months earlier and peak veliger densities were observed two months earlier than in Lake Erie. Annual maximum veliger density declined significantly during the study period (p < 0.0001). A population crash occurred as a result of thermal stress and variability of lake elevation. In summer 2011, water temperatures peaked at 34.3°C and lake elevation declined to the lowest level recorded during the previous 18 years, which resulted in desiccation of substantial numbers of settled mussels in littoral zones. Veliger spatial distributions were associated with physicochemical stratification characteristics. Veligers were observed in the deepest oxygenated water after lake stratification, which occurred in late spring. Results of this study indicate environmental conditions can influence variability of population sizes and spatial distributions of zebra mussels along the current southern frontier of their geographic range. Although the future population size trajectory and geographic range are uncertain, increased temperatures and intermittent, extreme droughts likely will affect spatio-temporal dynamics of established populations if zebra mussels spread farther into the southern and southwestern United States.

Oklahoma;Texas↗

Prevention of zebra mussel infestation and dispersal during aquaculture operations

The zebra mussel Dreissena polymorpha , an exotic invasive species, poses a major threat to North American fish management programs and the aquaculture industry. Fish hatcheries may become infected with zebra mussels from a variety of sources, including the water supply, fish shipments, boats, and equipment. The hatcheries could then serve as agents for the overland dispersal of zebra mussels into stocked waters and to other fish hatcheries. We evaluated the effectiveness and safety of aquaculture chemicals for use in controlling zebra mussels in fish hatcheries and preventing dispersal of veligers during fish transport. Chemicals were evaluated for use in fish transport and as disinfectants for ponds and equipment. Standardized static toxicity tests were conducted with representative species of warmwater, coolwater, and coldwater fishes and with larval (3‐d‐old veligers), early juvenile (settling larvae), and adult zebra mussels. Chemical concentrations and exposure durations were based on recommended treatment levels for fish, eggs, and ponds. Recommended treatment levels were also exceeded, if necessary, to establish lethal levels for zebra mussels of different developmental stages. Our results indicate that some chemicals currently in use in hatcheries may be effective for controlling zebra mussels in various operations. Chloride salts were the safest and most effective therapeutants tested for use in fish transport. The toxicity of chloride salts to fish varied among species and with temperature: only one treatment regime (sodium chloride at 10,000 mg/L) was safe to all fish species that we tested, but it was only effective on veliger and settler stages of the zebra mussel. Effective disinfectants were benzalkonium chloride for use on equipment and rotenone for use in ponds after fish are harvested. The regulatory status of the identified chemicals is discussed as well as several nonchemical control alternatives.

Progressive Fish-Culturist↗

Assessment of larval fish assemblages and nursery habitat in the St. Clair River delta

The St. Clair River delta, part of the St. Clair-Detroit River System (SCDRS), is the most fished coastal wetland area in the Laurentian Great Lakes and provides nursery habitat for a variety of fish species; however, few large-scale surveys of larval fish have been performed within the delta since the 1980s. Larval fish, zooplankton, and aquatic plants were sampled at 20 sites from May through July in 2010 and 2011 to characterize shallow channel and backwater delta habitats used by fish. The larval fish assemblage was sampled using active and passive gears (conical nets and light traps) and was dominated by Cyprinidae, Catostomidae, and Gobiidae. The microzooplankton assemblage was composed of rotifers, copepod nauplii, and Dreissena spp. veligers, while the macrozooplankton assemblage was composed of mostly cyclopoids and harpacticoids in May and cladocerans later in the season. Scirpus spp. dominated the plant assemblage in June and was replaced by Chara spp. in July. Seasonal compositional shifts were evident for larval fish, zooplankton, and plant assemblages, and greater densities of microzooplankton and cladocerans were typically found in backwater areas. Assemblage compositions were comparable to those in historical surveys but invasive Gobiidae and Dreissena spp. veligers now represent substantial proportions of the larval fish and zooplankton assemblages, respectively. Due to the high connectivity and advective nature of the SCDRS, understanding the larval fish assemblage dynamics of the delta can help inform estimates of system productivity.

Michigan↗

Feeding ecology of pelagic larval Burbot in Northern Lake Huron, Michigan

Burbot Lota lota are a key demersal piscivore across the Laurentian Great Lakes whose populations have declined by about 90% in recent decades. Larval Burbot typically hatch in the early spring and rely on abundant crustacean zooplankton prey. We examined the stomach contents of larval Burbot from inshore (&le;15 m) and offshore sites (37 and 91 m) in northern Lake Huron, Michigan. Concurrent zooplankton vertical tows at the same sites showed that the prey community was dominated by calanoid copepods, dreissenid mussel veligers, and rotifers. Burbot consumed mostly cyclopoid copepods, followed by copepod nauplii and calanoid copepods. Chesson's index of selectivity was calculated and compared among sites and months for individual Burbot. According to this index, larval Burbot exhibited positive selection for cyclopoid copepods and copepod nauplii and negative selection for calanoid copepods, cladocerans, rotifers, and dreissenid veligers. This selectivity was consistent across sites and throughout the sampling period. Burbot displayed little variation in their prey preferences during the larval stage, which suggests that the recent shifts in zooplankton abundance due to the invasion of the predatory zooplankter Bythotrephes longimanus and competition from invasive Rainbow Smelt Osmerus mordax could negatively impact larval Burbot populations.

Michigan↗