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At least 1,765 records · Page 98Linked to original sources

Reassociation and hybridization properties of DNAs from several species of fish

Reassociation and hybridization properties from spectrophotometric studies of DNAs from 10 species of fish indicate: 1. Great diversity in the amounts of repeated sequences in the genomes of different species - more specialized fish had less redundancy. 2. Large differences in the complexities of the DNAs - more specialized fish had less information. 3. Little homology between sequences of remotely related species but substantial homology between sequences of closely related species.

Comparative Biochemistry and Physiology, Part B: C↗

Amino acid nutrition of fishes: requirements and supplementation of diets

The purpose of this paper is: (1) to make a concise review of the published dietary requirements of fishes for amino acids, (2) to describe recent findings at the Tunison Laboratory concerning amino acid nutrition of trout, (3) to review specific signs of deficiency of amino acids, and (4) to discuss use of the fish egg amino acid pattern as a guideline to formulating new feeds or studying amino acid requirements of fishes for which there is limited information on their quantitative requirements.

Comparative Biochemistry and Physiology, Part B: C↗

Paramyxoviruses of fish

The first fish paramyxovirus was isolated from normal adult Chinook salmon returning to a coastal hatchery in Oregon in the fall of 1982. Subsequently, the virus was isolated from other stocks of adult Chinook salmon and one stock of adult coho salmon in California, Oregon, Washington and Alaska, leading to its designation as the Pacific salmon paramyxovirus ( PSPV ). The slow-growing virus can be isolated from tissues and ovarian fluids of healthy adult fish returning to spawn and apparently causes no clinical signs of disease or mortality. In 1995, a different and widely disseminated paramyxovirus was isolated from farmed Atlantic salmon in Norway and was designated as Atlantic salmon paramyxovirus ( ASPV ). Although this virus caused no disease or mortality when injected into juvenile Atlantic salmon, ASPV has been associated with proliferative gill inflammation in sea-reared yearling fish; however, additional infectious agents may be involved in the etiology of the condition. Sequence analysis of PSPV and ASPV isolates using the polymerase gene established their placement in the family Paramyxoviridae and has shown the two viruses to be closely related but sufficiently different from each other and from other known paramyxoviruses to possibly represent new genera within the family. The viruses can be diagnosed by isolation in cell culture with final confirmation by molecular methods. Other paramyxovirus-like agents have been observed or isolated from rainbow trout in Germany, from seabream in Japan associated with epithelial necrosis, from turbot in Spain associated with erythrocytic inclusion bodies and buccal/opercular hemorrhaging and from koi and common carp associated with gill necrosis in the European Union.

Book chapter↗

Paramyxoviruses of fish

The first fish paramyxovirus was isolated from normal adult Chinook salmon returning to a coastal hatchery in Oregon in the fall of 1982. Subsequently, the virus was isolated from other stocks of adult Chinook salmon and one stock of adult coho salmon in California, Oregon, Washington, and Alaska, leading to its designation as the Pacific salmon paramyxovirus (PsaPV). The slow-growing virus can be isolated from tissues and ovarian fluids of healthy adult fish returning to spawn and apparently causes no clinical signs of disease or mortality. In 1995 a different and widely disseminated paramyxovirus was isolated from farmed Atlantic salmon in Norway and was designated as Atlantic salmon paramyxovirus (AsaPV). Although this virus caused no disease or mortality when injected into juvenile Atlantic salmon, AsaPV has been associated with proliferative gill inflammation in sea-reared yearling fish; however, additional infectious agents may be involved in the etiology of the condition. Sequence analysis of PsaPV and AsaPV isolates using the polymerase gene established their placement in the family Paramyxoviridae and has shown the two viruses to be closely related but sufficiently different from each other and from other known paramyxoviruses to represent a new genus within the family. The viruses can be diagnosed by isolation in cell culture with final confirmation by molecular methods. Other paramyxovirus-like agents have been observed or isolated from rainbow trout in Germany, from seabream in Japan associated with epithelial necrosis, from turbot in Spain associated with erythrocytic inclusion bodies and buccal/opercular hemorrhaging and from koi and common carp associated with gill necrosis in the European Union.

Book chapter↗

Survival of cool and warm freshwater fish following chloramine-T exposure

Chloramine-T is presently available in the USA to control mortalities associated with bacterial gill disease or external columnaris only through an Investigational New Animal Drug Permit authorized by the US Food and Drug Administration (FDA). Its US approval hinges on FDA's acceptance of several key data, including those describing animal safety. Chloramine-T is presently applied in US aquaculture, by permit only, once daily on consecutive or alternate days for 1??h at 10 to 20??mg/L to control mortalities associated with bacterial gill disease or external columnaris. Our objective was to determine the safety of chloramine-T bath exposures at multiples of the proposed maximum treatment concentration (i.e., 0, 20, 60, 100, and 200??mg/L) administered on four consecutive days at 20????C to lake sturgeon Acipenser fulvescens, northern pike Esox lucius, and walleye Sander vitreum, or at 27????C to channel catfish Ictalurus punctatus, and largemouth bass Micropterus salmoides. All fish were tested as five to eight week old fry except for walleye and channel catfish which were tested as both fry and fingerling (fingerlings were at least four weeks older than the fry tested). Walleye and channel catfish were selected to evaluate the effects of life stage (fry vs. fingerling), temperature (walleye - 15, 20, or 25????C; channel catfish - 22, 27, or 32????C), exposure duration (60 vs. 180??min), and water chemistry (walleye only - reconstituted soft water vs. well water). Except for channel catfish fry, survival was significantly reduced only when fish were treated at 100 or 200??mg/L. Channel catfish fry survival was significantly reduced when exposed at 60??mg/L for 180??min at 27????C. Based on our mortality data, chloramine-T administered once daily for 60??min on four consecutive days at concentrations of up to 20??mg/L is not likely to adversely affect survival of cool or warmwater fish cultured in freshwater. Crown Copyright ?? 2007.

Aquaculture↗

Occurrence and tissue distribution of per- and polyfluoroalkyl substances (PFAS) in fishes from waterbodies with point and non-point sources in Massachusetts, USA

Per- and polyfluoroalkyl substances (PFAS) are persistent environmental contaminants with known bioaccumulative and toxic effects in aquatic ecosystems. This study assessed site-specific differences in PFAS contamination in fish from Ashumet Pond, Sudbury River, and Great Herring Pond (reference site) in Massachusetts. Fish from Ashumet Pond exhibited the highest PFAS concentrations, particularly perfluorooctane sulfonate (PFOS), which exceeded levels in plasma almost 650 times those at the reference site. Principal component analysis identified distinct PFAS profiles at each site, reflecting localized contamination sources. Temporal analysis at Ashumet Pond revealed a substantial increase in plasma PFOS and perfluorodecanoic acid (PFDA) from 2020 to 2022. Tissue distribution analyses showed the highest PFAS concentrations in plasma, followed by liver and muscle, consistent with PFAS binding affinity for blood proteins. Species-specific differences in PFAS bioaccumulation were observed, with largemouth bass ( Micropterus nigricans ) exhibiting higher body burdens than banded killifish ( Diaphanus fundulus ), likely due to trophic position and dietary exposure. Histopathological assessments and gene transcript analyses revealed associations between PFAS exposure and inflammatory responses, oxidative stress, endocrine disruption, and immune-related pathways, with the most pronounced molecular effects observed at the downstream site of the Sudbury River. This study underscores the importance of understanding site-specific contamination sources, exposure pathways, and biological effects of PFAS in fish. These findings would benefit from additional research on sediment contamination, temporal analyses at each site, trophic transfer, and transcriptomic analyses across multiple organs to further elucidate PFAS toxicity mechanisms and guide remediation efforts.

Massachusetts↗

Stream fish colonization but not persistence varies regionally across a large North American river basin

Many species have distributions that span distinctly different physiographic regions, and effective conservation of such taxa will require a full accounting of all factors that potentially influence populations. Ecologists recognize effects of physiographic differences in topography, geology and climate on local habitat configurations, and thus the relevance of landscape heterogeneity to species distributions and abundances. However, research is lacking that examines how physiography affects the processes underlying metapopulation dynamics. We used data describing occupancy dynamics of stream fishes to evaluate evidence that physiography influences rates at which individual taxa persist in or colonize stream reaches under different flow conditions. Using periodic survey data from a stream fish assemblage in a large river basin that encompasses multiple physiographic regions, we fit multi-species dynamic occupancy models. Our modeling results suggested that stream fish colonization but not persistence was strongly governed by physiography, with estimated colonization rates considerably higher in Coastal Plain streams than in Piedmont and Blue Ridge systems. Like colonization, persistence was positively related to an index of stream flow magnitude, but the relationship between flow and persistence did not depend on physiography. Understanding the relative importance of colonization and persistence, and how one or both processes may change across the landscape, is critical information for the conservation of broadly distributed taxa, and conservation strategies explicitly accounting for spatial variation in these processes are likely to be more successful for such taxa.

Alabama, Florida, Georgia↗

Fish body midline segmentation using binary search

Body and caudal fin locomotion is ubiquitous in aquatic vertebrates, and kinematic models describing it are used in robotics, biomechanics and fisheries research. This paper presents a new algorithm to translate continuous body midlines of fish into a series of interconnected segments by identifying favorable joint positions along the body. The algorithm employs binary search to generate parsimonious kinematic models, aiming at minimizing the number of segments yet keeping approximation error below a user-defined threshold. To achieve this, the algorithm maximizes the length of each segment by determining the most distal joint position through repetitive shrinking of the search space. Theoretical and empirical analysis using two different datasets show that the binary search algorithm is substantially faster when compared to segment growing algorithm, which employs linear search to generate its models. There is four-fold improvement in computation time when generating models with less than 10 segments, which are typically sufficient to describe fish and fish-inspired robot movements. Furthermore, the multi-segment models generated by the binary search algorithm matched the ground truth models obtained through dynamic programming in over 97% of cases, and on average, contained one fewer segment than those produced by the Ramer–Douglas–Peucker algorithm, which is widely used in curvature simplification tasks. Our findings suggest that the binary search algorithm provides a computationally efficient approach for generating compact kinematic models and may facilitate the analysis of large datasets with high temporal and spatial resolution.

Computers and Electronics in Agriculture↗

Temporal changes in aquatic-invertebrate and fish assemblages in streams of the north-central and northeastern U.S.

Many management agencies seek to evaluate temporal changes in aquatic assemblages at monitoring sites, but few have sites with ecological time series that are long enough for this purpose. Trends in aquatic-invertebrate and fish assemblage composition were assessed at 27 long-term monitoring sites in the north-central and northeastern United States. Temporal changes were identified using serial trend analysis. Sites with significant serial trends were further evaluated by relating explanatory environmental variables (e.g., streamflow, habitat, and water chemistry) to changes in assemblage composition. Significant trends were found at 19 of 27 study sites; however, differences in the sensitivity of the aquatic fauna to environmental stressors were identified. For example, significant trends in fish assemblages were found at more sites (15 of 27) than for aquatic-invertebrate assemblages (10 of 27 sites). In addition, trends in the invertebrate assemblage were most often explained by changes in streamflow processes (e.g., duration and magnitude of low- and high-flows, streamflow variability, and annual rates of change), whereas trends in the fish assemblage were more related to changes in water chemistry. Results illustrate the value of long-term monitoring for the purpose of assessing temporal trends in aquatic assemblages. The ability to detect trends in assemblage composition and to attribute these changes to environmental factors is necessary to understand mechanistic pathways and to further our understanding of how incremental anthropogenic alterations modify aquatic assemblages over time. Finally, this study's approach to trends analysis can be used to better inform the design of monitoring programs as well as support the ongoing management needs of stakeholders, water-resource agencies, and policy makers.

Connecticut, Illinois, Indiana, Iowa, Massachusett↗

Assessment of multi-stressors on compositional turnover of diatom, invertebrate and fish assemblages along an urban gradient in Pacific Northwest streams (USA)

This study is part of the regional stream-quality assessment (RSQA) conducted by the U.S. Geological Survey (USGS) National Water Quality Assessment (NAWQA) project. The purpose of this study is to examine small streams along land-use and stressor gradients at the regional scale and to evaluate the relative importance of instream stressors on diatom, macroinvertebrate, and fish assemblages. In 2015, the RSQA project assessed stream quality in 82 wadeable streams that were selected along an urban land-use gradient in the Pacific Northwest Region (PNW) of the United States. This study evaluates the effects of four major categories of measured instream stressors – flow (i.e. alteration), water quality, habitat, and contaminants (in water and sediment) – on stream biota. We used gradient forest (GF) models to evaluate taxon specific responses to the various stressors for the three biotic assemblages. Results for diatom, invertebrate and fish assemblages showed that several environmental variables including substrate size, dissolved oxygen, and two or more different contaminants were selected in each of the GF models. In general, all three assemblages were negatively associated with any contaminant measures above zero, except the more tolerant taxa in each assemblage, which responded positively to contaminants. Total nitrogen (TN) and total phosphorus (TP) were important in both the diatom and invertebrate GF models but not in the fish models, which were related to temperature and stream flow. TP and TN were the top two variables for diatom GF models and various taxa responded at a range of nutrient concentrations; however, some taxa responded at low concentrations, for example around 0.02 for TP and 0.5 mg/L for TN. In general, the three biotic assemblages responded to multiple stressors following general patterns of known sensitive versus tolerant taxa for each of the biotic groups studied, yet the GF models allow us to explore taxon specific responses. For example, most of the sensitive Ephemeroptera, Plecoptera, Trichoptera invertebrate taxa (EPT) responded negatively when any contaminant increased above zero; yet some taxa such as the tolerant Trichoptera Cheumatopsyche responded positively to contaminants and many of the other stressors. The findings of this study demonstrate the value of using multiple assemblages to monitoring stressor gradients associated with urban stream systems and the importance of evaluating the responses of individual taxa to stressors.

Washington, Oregon↗

An integrated analysis for estimation of survival, growth, and movement of unmarked juvenile anadromous fish

Managers invest substantial resources to promote recovery of declining anadromous fish stocks. Recovery strategies are manifold and often include management actions intended to stimulate somatic growth, increase in-river survival, and motivate juvenile outmigration during favorable environmental conditions. Evaluating the efficacy of these management actions is difficult, however, because monitoring data that explicitly track individuals from egg deposition to juvenile outmigration are typically lacking. We developed an integrated population model that links two different and often collected types of anadromous fish monitoring data: spawning ground surveys and rotary screw trap juvenile catch data. The integrated model accounts for incomplete detection and uses the two sources of data to estimate juvenile demographic parameters in a multistate framework. We evaluated the model's performance using simulated data under a range of conditions typically encountered in similar surveys. Simulation results indicated that the model estimated juvenile survival, growth, and movement with no-to-minimal bias (i.e., ≥ 50 % of simulations ± 0–0.05). As an example case study, we fit the model to empirical fall-run Chinook Salmon ( Oncorhynchus tshawytscha ) monitoring data collected in California's Central Valley, U.S.A. In doing so, we evaluated the influence of environmental conditions (e.g., discharge, water temperature) and habitat availability on juvenile demographic rates. We demonstrated that through our integrated approach we could estimate state transition probabilities that are typically inestimable for naturally produced, unmarked juvenile fish when using traditional statistical approaches to analyze these types of monitoring data. Furthermore, the structure of our model can serve as a useful foundation for decision-support models within adaptive management programs by directly linking management actions, decision-support-model predictions, and monitoring.

California↗

Sediment transport modeling in Lake Ontario embayments: Impacts on fish spawning substrates

Anthropogenically-driven sedimentation changes have had adverse environmental impacts on aquatic environments, including reductions in fish spawning habitats in embayments worldwide. This study was motivated by the need to understand the impacts of waves and current-driven sedimentation patterns on traditional spawning areas and their effect on sustainable fish reproduction in the Great Lakes. Coupled hydrodynamic, wave, and sediment transport models were developed within the Delft3D-SWAN (DS) framework to predict sedimentation patterns in two embayments in Lake Ontario, Sodus Bay and Chaumont Bay, that have been historically important fish spawning habitats. These bays, with distinct geomorphic characteristics and connectivity to Lake Ontario, offer an opportunity to examine how wind-generated waves and currents impact bed shear stress and subsequent sedimentation patterns. Areas experiencing greater wave-induced bed shear stress were identified and compared between the two bays. Simulated sediment transport patterns showed notable erosion near the lake-bay connections and increased deposition in the inner areas of both embayments. Observed Cisco embryo deposition corresponded to regions of high sheer stress and lower sedimentation, indicating physical attributes in those areas that are important for embryo survival. These results show where sediment settling and erosion occur in the two bays and highlight potential impacts on traditional spawning areas.

New York↗

Persistence of organochlorine chemical residues in fish from the Tombigbee River (Alabama, USA): Continuing risk to wildlife from a former DDT manufacturing facility

Organochlorine pesticide and total polychlorinated biphenyl (PCB) concentrations were measured in largemouth bass from the Tombigbee River near a former DDT manufacturing facility at McIntosh, Alabama. Evaluation of mean p,p???- and o,p???-DDT isomer concentrations and o,p???- versus p,p???-isomer proportions in McIntosh bass indicated that DDT is moving off site from the facility and into the Tombigbee River. Concentrations of p,p???-DDT isomers in McIntosh bass remained unchanged from 1974 to 2004 and were four times greater than contemporary concentrations from a national program. Total DDT in McIntosh bass exceeded dietary effect concentrations developed for bald eagle and osprey. Hexachlorobenzene, PCBs, and toxaphene concentrations in bass from McIntosh also exceeded thresholds to protect fish and piscivorous wildlife. Whereas concentrations of DDT and most other organochlorine chemicals in fish have generally declined in the U.S. since their ban, concentrations of DDT in fish from McIntosh remain elevated and represent a threat to wildlife.

Environmental Pollution↗

PFAS contamination in Florida estuarine fishes: Levels, patterns, and estimated human and ecological health risks

Per- and polyfluoroalkyl substances (PFAS) pose a significant health threat due to their environmental persistence and toxicity. While PFAS contamination is widespread in Florida, the state currently lacks fish consumption advisories (FCAs) for these compounds, despite existing FCAs for legacy pollutants. This study quantified 40 PFAS in edible muscle tissue from 264 fish (16 species) across four estuaries along Florida's Atlantic coast to assess ecological and human health risks. The highest concentrations of total PFAS were found in Red Drum ( Sciaenops ocellatus ; 0.209 – 51.6 ng/g wet weight) and Spotted Seatrout ( Cynoscion nebulosus; 2.01 – 24.3 ng/g ww) in the Indian River Lagoon, where up to 68% of Red Drum and 75% of Spotted Seatrout exceeded ecological quality standards, indicating potential impacts on predators. PFOS was the predominant PFAS, driving ecological and human health risks. Estimated daily intakes (EDIs) of PFOS exceeded the EPA reference dose (RfD) by up to 3 orders of magnitude, with the highest exposures concentrated in Red Drum and Spotted Seatrout from the Indian River Lagoon. These findings highlight that PFAS monitoring of tissue concentrations in fish across Florida's freshwater and marine systems would be needed for accurately quantifying exposure risks. Such efforts are fundamental to informing regulatory frameworks regarding FCAs and ensuring the long-term protection of aquatic ecosystems and human health.

Florida↗

Evaluation of a field protocol for internally-tagging fish predators using difficult-to-tag ictalurid catfish as examples

Tagging protocols that result in high tag retention will benefit fisheries professionals who use telemetry data. Ictalurid catfish historically have had very poor telemetry tag retention. Here, we use these difficult-to-tag taxa to address two research objectives. First, we evaluated our field-based internal tagging methodology by quantifying six tag retention metrics using data from 48 acoustically-tagged blue catfish ( Ictalurus furcatus ) released into Milford Reservoir, KS, USA. Second, to better understand the results of this field evaluation, we evaluated tag retention and survival in the hatchery for the closely-related channel catfish ( I. punctatus ). Field and hatchery studies provided complementary information. Eighty percent of tagged blue catfish were detected at the end of our 5-mo field study (1,139,402 = total detections; 24,243 = average detections per fish). A 13-week hatchery evaluation of our field methodology also had high tag retention (100%). Hatchery treatments that used a lateral-ventral incision had higher tag retention and survival than those treatments that included a mid-ventral incision. Time invested in training, protocol refinement, and field organization were also important. Furthermore, monitoring the surgical history and recovery of individual fish allowed us to correct problems before mortality occurred. Because a need exists for a range of internal tagging methodologies that are effective on an assortment of fish species tagged under a variety of circumstances, our high tag retention methodology may be useful to the expanding research community that studies native and non-native catfish, as well as, other telemetry researchers who seek beneficial refinements to their tagging protocols.

Kansas↗

Assessing the spawning ecology of fish in situ using a benthic pump sampler

In situ observations of incubating fish eggs can identify spawning sites and spawning habitat preferences, informing the ecology of fishes with benthic eggs. Suction pumps have been used to sample benthic-incubating, non-adhesive fish eggs, yet their sampling efficiency is not well known. Imperfect or systematically variable egg detection could bias resulting ecological inference if left unaddressed. Here we present results from replicate field trials and examine the effects of varying substrate type, intake design, sampling effort, and egg density on the ability of a gasoline-powered diaphragm pump, rated for 66 gal/min (approx. 250 L/min), to detect egg presence and estimate relative or absolute abundance. A wider box-shaped intake was effective at detecting the presence of eggs on fine, silty substrates, but had limited effectiveness on larger-grained substrates. A narrower cone-shaped intake consistently detected the presence of eggs on silt, gravel, and shallow cobble, and demonstrated potential to measure relative and absolute egg abundance. Neither intake design was able to collect eggs from deep interstitial spaces (e.g. several layers of cobble), indicating that there are limitations to the types of substrates on which benthic sampling pumps can operate. Both intake designs often collected eggs from outside the edge of the intake opening. Most eggs were collected within a two minute period, but increasing pumping time to four minutes produced better egg detection outcomes. Our results suggest that stationary benthic sampling pumps are viable tools for directly sampling incubating eggs on most substrates, but have imperfect and varying sampling efficiency resulting from intake design and substrate type.

Fisheries Research↗

Hydroacoustic survey standardization: Inter-vessel differences in fish densities and potential effects of vessel avoidance

Hydroacoustics is used broadly to assess fish populations in marine and freshwater systems. Large-scale surveys often employ multiple vessels to complete a survey. Vessels can be a source of variation in multi-vessel surveys, and accounting for this variation is critical to precise and accurate assessments, whether as indices or measures of absolute abundance. We examined areal and volumetric density estimates, area backscattering coefficient (ABC), and mean target strength (TS) produced by three vessels in Lake Erie along three cross-lake transects in July 2014. Our study showed positive correlation in areal ABC, mean TS, and density among vessels; however, there was an almost 30 % higher density obtained with the largest vessel, on average. As a result, vessel choice could contribute to variation and/or bias in forage fish surveys deploying multi-vessels conducted on Lake Erie. Additionally, in some situations, differential depth patterns of fish contributed to vessel related differences in survey results suggesting a difference in vessel avoidance behaviors among targeted forage species, primarily emerald shiner Notropis atherinoides and rainbow smelt Osmerus mordax . This work demonstrates the potential magnitude of differences among vessels and highlights the need to identify and account for or otherwise control for these effects when monitoring or managing fisheries through standardized hydroacoustic assessments surveys.

Lake Erie↗

Optimizing per vessel hour capture efficiency for rare, heterogeneously distributed fishes: Invasive grass carp Ctenopharyngodon idella in the Sandusky River

Natural resources management is often concerned with conserving rare-native or controlling rare-invasive fishes. Informing and assessing conservation and control efforts frequently requires information from captures. When little is understood about spatial and temporal fish distributions, captures can be infrequent and costly. If successful management depends on effective management response, optimizing for efficiency may be the difference between success and failure. We compared per vessel hour capture efficiencies for invasive grass carp ( Ctenopharyngodon idella ) between two methods: electrofishing-only (electrofishing) and in combination with a trammel net (combination). Capture and effort information including 174 captures from 1853 capture attempts from 1706 total hours of effort in the Sandusky River, OH, USA from 2020–2023 was used to fit a generalized linear model. Captures were allowed to vary by river kilometer, month, and year to account for unequal capture rates and effort. Captures were offset by total vessel hours or the count of independent efforts to compare methods that prioritize detection at a single location (e.g., combination) to methods that prioritize exploiting more locations (e.g., electrofishing). Including trammel nets was intended to increase single site detection, but we found that electrofishing-only was at least 2.4x more efficient (catch per vessel hour) than when combined with a trammel net with no significant difference in catch per removal effort. Complex methods intended to increase single site detection may reduce the number of efforts completed. Therefore, overall capture efficiency and total capture numbers for rare fish may be increased through methods that prioritize per-hour efficiency.

Ohio↗