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Brain cholinesterase activity in fledgling starlings: Implications for monitoring exposure of songbirds to ChE inhibitors

Exposure of songbirds to organophosphate and carbamate pesticides is often determined by comparing the brain cholinesterase (ChE) activity of individuals that have been exposed with that of unexposed birds of the same species (e.g., see Busby et al. 1981, 1982; Hamilton et al. 1981). Knowledge of the normal pattern of ChE development in the brains of nestling and fledgling passerines is a necessary prerequisite for monitoring exposure to ChE inhibitors, and assessing the impacts of these inhibitors on the reproductive success and survival of songbirds. In a previous study, Grue et al. (1981) reported that ChE activity in the brains of wild nestling European starlings (Sturnus vulgaris), 3 to 18 days old, varied significantly with age and appeared to increase toward adult levels at a constant rate. Grue et al. (1981) hypothesized that if brain ChE activity continued to develop at a constant rate, levels in the brains of fledgling starlings about 28 days old would be comparable to those in the brains of adults. The present study was conducted to test this hypothesis.

Bulletin of Environmental Contamination and Toxico↗

Seasonal brain acetylcholinesterase activity in three species of shorebirds overwintering in Texas

There was no seasonal variation in average brain AChE activity for the 3 species of wild birds collected between October and February. Further work needs to be done, however, covering an even broader time frame which includes the reproductive cycle. It appears that some birds feeding at the mouth of an agricultural drain, at some distance from the nearest pesticide applications, were affected by AChE inhibitors.

Bulletin of Environmental Contamination and Toxico↗

Biomonitors of stream quality on agricultural areas: fish versus invertebrates

Although the utility of using either fish or benthic invertebrates as biomonitors of stream quality has been clearly shown, there is little comparative information on the usefulness of the groups in any particular situation. We compared fish to invertebrate assemblages in their ability to reflect habitat quality of sediment-impacted streams in agricultural regions of northeast Missouri, USA. Habitat quality was measured by a combination of substrate composition, riparian type, buffer strip width, and land use. Invertebrates were more sensitive to habitat differences when structural measurements, species diversity and ordination, were used. Incorporating ecological measurements, by using the Index of Biological Integrity, increased the information obtained from the fish assemblage. The differential response of the two groups was attributed to the more direct impact of sediments on invertebrate life requisites; the impact of sedimentation on fish is considered more indirect and complex, affecting feeding and reproductive mechanisms.

Environmental Management↗

Nesting habitat of Belding’s Savannah sparrows in coastal salt marshes

Although the Belding’s Savannah sparrow ( Passerculus sandwichensis beldingl ) is listed as endangered in California, little is known about the factors that affect its abundance and distribution. Numbers of breeding pairs, nesting territory sizes, and vegetation characteristics were measured at fourteen study plots in two southern California coastal wetlands, Tijuana Estuary and Los Peñasquitos Lagoon. Sparrows nested in middle salt marsh habitat in areas of dense pickleweed ( Salicornia virginica ) or saltgrass ( Distichlis spicata ). No nesting territories were found in transitional upland or low marsh habitat. Territory sizes were highly variable and significantly larger at Tijuana Estuary. Territory size may be related to nest site availability and water levels at time of establishment. More research is needed on the effects of habitat quality on reproductive success and territory size.

California↗

Downstream effects of dams on channel geometry and bottomland vegetation: Regional patterns in the Great Plains

The response of rivers and riparian forests to upstream dams shows a regional pattern related to physiographic and climatic factors that influence channel geometry. We carried out a spatial analysis of the response of channel geometry to 35 dams in the Great Plains and Central Lowlands, USA. The principal response of a braided channel to an upstream dam is channel-narrowing, and the principal response of a meandering channel is a reduction in channel migration rate. Prior to water management, braided channels were most common in the southwestern Plains where sand is abundant, whereas meandering channels were most common in the northern and eastern Plains. The dominant response to upstream dams has been channel-narrowing in the southwestern Plains (e.g., six of nine cases in the High Plains) and reduction in migration rate in the north and east (e.g., all of twelve cases in the Missouri Plateau and Western Lake Regions). Channel-narrowing is associated with a burst of establishment of native and exotic woody riparian pioneer species on the former channel bed. In contrast, reduction in channel migration rate is associated with a decrease in reproduction of woody riparian pioneers. Thus, riparian pioneer forests along large rivers in the southwestern Plains have temporarily increased following dam construction while such forests in the north and east have decreased. These patterns explain apparent contradictions in conclusions of studies that focused on single rivers or small regions and provide a framework for predicting effects of dams on large rivers in the Great Plains and elsewhere. These conclusions are valid only for large rivers. A spatial analysis of channel width along 286 streams ranging in mean annual discharge from 0.004 to 1370 cubic meters per second did not produce the same clear regional pattern, in part because the channel geometries of small and large streams are affected differently by a sandy watershed.

Great Plains↗

Pollen dispersal by catapult: Experiments of Lyman J. Briggs on the flower of mountain laurel

The flower of Kalmia latifolia L. employs a catapult mechanism that flings its pollen to considerable distances. Physicist Lyman J. Briggs investigated this phenomenon in the 1950s after retiring as longtime director of the National Bureau of Standards, attempting to explain how hydromechanical effects inside the flower’s stamen could make it possible. Briggs’s unfinished manuscript implies that liquid under negative pressure generates stress, which, superimposed on the stress generated from the flower’s growth habit, results in force adequate to propel the pollen as observed. With new data and biophysical understanding to supplement Briggs’s experimental results and research notes, we show that his postulated negative-pressure mechanism did not play the exclusive and crucial role that he credited to it, though his revisited investigation sheds light on various related processes. Important issues concerning the development and reproductive function of Kalmia flowers remain unresolved, highlighting the need for further biophysical advances.

Physics in Perspective↗

Polychlorinated biphenyls, mercury, and potential endocrine disruption in fish from the Hudson River, New York, USA

Tissue residues of total mercury (Hg), total polychlorinated biphenyls (PCBs), and lipid-based PCBs; plasma concentrations of endocrine biomarkers; and reproductive and histologic biomarkers were assessed in 460 carp (Cyprinus carpio), bass (Micropterus salmoides and Micropterus dolomieui), and bullhead (Ameiurus nebulosus) collected from eight sites across the Hudson River Basin in the spring of 1998 to determine if endocrine disruption was evident in resident fish species and to evaluate contaminant-biomarker interrelations. Total PCBs in bed sediments (maximum 2,500 ??g kg-1) could explain 64 to 90% of the variability in lipid-based PCB residues in tissues (maximum 1,250 ??g PCB g-lipid-1) of the four species. The 17??-estradiol to 11-ketotestosterone ratio, typically less than 1.0 in male fish and greater than 1.0 in females, exceeded 1.4 in all male largemouth bass and 35% of male carp and bullhead at one site 21 km downstream from a major PCB source. Endocrine biomarkers were significantly correlated with total Hg in female smallmouth bass and carp, and with lipid-based PCBs in males of all four species. Empirical evidence of endocrine modulation in blood plasma of male and female fish from sites with and without high PCB residues in bed sediments and fish tissues suggest that PCBs, Hg, or other contaminants may disrupt normal endocrine function in fish of the Hudson River. ?? Eawag, 2006.

Aquatic Sciences↗

Fertilization of eggs of Lake Michigan lake trout Salvelinus namaycush in lake water: Effect of PCBs (Aroclor 1254)

Various studies indicate that PCBs appear to have an adverse effect on the viability of fertilized eggs and subsequent early life stages of lake trout and related species. Our tests detected no impairment of fertilization of lake trout eggs in PCB-dosed lake water. The concentration of PCBs in the fertilization medium that we used was more than 20 times as high as estimated ambient levels in southeastern Lake Michigan and it appears unlikley that ambient levels of PCBs in the water at fertilization would contribute significantly to the apparent widespread reproductive failure of lake trout there.

Bulletin of Environmental Contamination and Toxico↗

Changes in size and trends of North American sea duck populations associated with North Pacific oceanic regime shifts

Broad-scale multi-species declines in populations of North American sea ducks for unknown reasons is cause for management concern. Oceanic regime shifts have been associated with rapid changes in ecosystem structure of the North Pacific and Bering Sea. However, relatively little is known about potential effects of these changes in oceanic conditions on marine bird populations at broad scales. I examined changes in North American breeding populations of sea ducks from 1957 to 2011 in relation to potential oceanic regime shifts in the North Pacific in 1977, 1989, and 1998. There was strong support for population-level effects of regime shifts in 1977 and 1989, but little support for an effect of the 1998 shift. The continental-level effects of these regime shifts differed across species groups and time. Based on patterns of sea duck population dynamics associated with regime shifts, it is unclear if the mechanism of change relates to survival or reproduction. Results of this analysis support the hypothesis that population size and trends of North American sea ducks are strongly influenced by oceanic conditions. The perceived population declines appear to have halted >20 years ago, and populations have been relatively stable or increasing since that time. Given these results, we should reasonably expect dramatic changes in sea duck population status and trends with future oceanic regime shifts.

Marine Biology↗

Avian predator buffers against variability in marine habitats with flexible foraging behavior

How well seabirds compensate for variability in prey abundance and composition near their breeding colonies influences their distribution and reproductive success. We used tufted puffins ( Fratercula cirrhata ) as forage fish samplers to study marine food webs from the western Aleutian Islands (53°N, 173°E) to Kodiak Island (57°N, 153°W), Alaska, during August 2012–2014. Around each colony we obtained data on: environmental characteristics (sea surface temperature and salinity, seafloor depth and slope, tidal range, and chlorophyll- a ), relative forage fish biomass (hydroacoustic backscatter), and seabird community composition and density at-sea. On colonies, we collected puffin chick-meals to characterize forage communities and determine meal energy density, and measured chicks to obtain a body condition index. There were distinct environmental gradients from west to east, and environmental variables differed by ecoregions: the (1) Western-Central Aleutians, (2) Eastern Aleutians, and, (3) Alaska Peninsula. Forage fish biomass, species richness, and community composition all differed markedly between ecoregions. Forage biomass was strongly correlated with environmental gradients, and environmental gradients and forage biomass accounted for ~ 50% of the variability in at-sea density of tufted puffins and all seabird taxa combined. Despite the local and regional variability in marine environments and forage, the mean biomass of prey delivered to puffin chicks did not differ significantly between ecoregions, nor did chick condition or puffin density at-sea. We conclude that puffins can adjust their foraging behavior to produce healthy chicks across a wide range of environmental conditions. This extraordinary flexibility enables their overall success and wide distribution across the North Pacific Ocean.

Aleutian Islands, Gulf of Alaska↗

Quantifying salinity and season effects on eastern oyster clearance and oxygen consumption rates

There are few data on Crassostrea virginica physiological rates across the range of salinities and temperatures to which they are regularly exposed, and this limits the applicability of growth and production models using these data. The objectives of this study were to quantify, in winter (17 °C) and summer (27 °C), the clearance and oxygen consumption rates of C. virginica from Louisiana across a range of salinities typical of the region (3, 6, 9, 15 and 25). Salinity and season (temperature and reproduction) affected C. virginica physiology differently; salinity impacted clearance rates with reduced feeding rates at low salinities, while season had a strong effect on respiration rates. Highest clearance rates were found at salinities of 9–25, with reductions ranging from 50 to 80 and 90 to 95% at salinities of 6 and 3, respectively. Oxygen consumption rates in summer were four times higher than in winter. Oxygen consumption rates were within a narrow range and similar among salinities in winter, but varied greatly among individuals and salinities in summer. This likely reflected varying stages of gonad development. Valve movements measured at the five salinities indicated oysters were open 50–60% of the time in the 6–25 salinity range and ~ 30% at a salinity of 3. Reduced opening periods, concomitant with narrower valve gap amplitudes, are in accord with the limited feeding at the lowest salinity (3). These data indicate the need for increased focus on experimental determination of optimal ranges and thresholds to better quantify oyster population responses to environmental changes.

Marine Biology↗

Measuring carbon and nitrogen bioassimilation, burial, and denitrification contributions of oyster reefs in Gulf coast estuaries

The eastern oyster ( Crassostrea virginica ) and the reefs they create provide significant ecosystem services. This study measured their possible role in nutrient mitigation through bioassimilation, burial, and oyster-mediated sediment denitrification in near-shore shallow water (< 1 m water depth) and deep-water (> 1 m water depth) oyster reefs in Louisiana. Nitrogen (N) and carbon (C) in shell and tissue differed by oyster reproductive status, size, and habitat type. Changes in tissue percent N and C post-spawning combined with significant reductions in tissue dry weight from the release of gametes, resulted in 20 and 46% reductions in tissue N and C load (mg), respectively, for a 100-mm oyster. Oyster reefs did not enhance burial rates, with burial range rates estimated at 1.4–2.6 g N m −2 year −1 , and 26.9–43.8 g C m −2 year −1 . Closed-system ex situ incubations indicated net denitrification in all habitat types studied, with the highest rates exceeding 600 µmol N m −2 h −1 during the summer, but no enhancement attributable to oyster reefs specifically. Within the highly productive, organic-rich wetland complex systems of coastal Louisiana, oyster reefs were not associated with enhanced denitrification, likely due to the organic-rich setting, and redundant supplies of organic nitrogen and carbon from adjacent marshes. Context remains critical in determining ecosystem provision of habitats, and efforts to extrapolate and predict nitrogen removal across locations necessitates consideration of local conditions. Considering the large extent of reefs and oyster production across coastal Louisiana, oyster habitats may still contribute to N and C mitigation, but their unique contribution likely comes from bioassimilation, and removal of the oysters from the system.

Louisiana↗

Metal concentrations in zebra mussels and sediments from embayments and riverine environments of eastern Lake Erie, southern Lake Ontario, and the Niagara River

Concentrations of 14 metals were studied in the soft tissues of zebra mussels ( Dreissena polymorpha ) and sediments from 16 Great Lakes embayments and riverine environments. Samples were collected in 1993 and 1994 during the early and late autumn period when the body mass of mussels is least affected by reproductive activities. There was a significant difference in geometric mean concentrations of all metals except Cu in mussels sampled from different sites, and there was a significant difference in the geometric mean concentrations of all metals but Cd, Mn, and Zn between years. The higher metal concentrations in mussels from this study were generally similar to those in mussels from contaminated European and U.S. locations, and those with lower concentrations were similar to those from uncontaminated European and U.S. locations. Geometric mean sediment concentrations of all metals differed significantly among sites. Sediment concentrations of metals from some sites were above EPA guidelines for moderately polluted harbor sediments. Sites where zebra mussels had higher concentrations of Al, Cr, and V tended to be the same sites as those where sediment concentrations of these metals were also higher. However, there was not a significant statistical relationship between concentrations of metals in zebra mussels and sediments, except for Mg.

Lake Erie, Lake Ontario, Niagara River↗

Assessing contaminant sensitivity of endangered and threatened aquatic species: Part III. Effluent toxicity tests

Toxicity tests using standard effluent test procedures described by the U.S. Environmental Protection Agency were conducted with Ceriodaphnia dubia, fathead minnows (Pimephales promelas), and seven threatened and endangered (listed) fish species from four families: (1) Acipenseridae: shortnose sturgeon (Acipenser brevirostrum); (2) Catostomidae; razorback sucker (Xyrauchen texanus); (3) Cyprinidae: bonytail chub (Gila elegans), Cape Fear shiner (Notropis mekistocholas) Colorado pikeminnow (Ptychocheilus lucius), and spotfin chub (Cyprinella monacha); and (4) Poecillidae: Gila topminnow (Poeciliopsis occidentalis). We conducted 7-day survival and growth studies with embryo-larval fathead minnows and analogous exposures using the listed species. Survival and reproduction were also determined with C. dubia. Tests were conducted with carbaryl, ammonia-or a simulated effluent complex mixture of carbaryl, copper, 4-nonylphenol, pentachlorophenol and permethrin at equitoxic proportions. In addition, Cape Fear shiners and spotfin chub were tested using diazinon, copper, and chlorine. Toxicity tests were also conducted with field-collected effluents from domestic or industrial facilities. Bonytail chub and razorback suckers were tested with effluents collected in Arizona whereas effluent samples collected from North Carolina were tested with Cape Fear shiner, spotfin chub, and shortnose sturgeon. The fathead minnow 7-day effluent test was often a reliable estimator of toxic effects to the listed fishes. However, in 21 % of the tests, a listed species was more sensitive than fathead minnows. More sensitive species results varied by test so that usually no species was always more or less sensitive than fathead minnows. Only the Gila topminnow was consistently less sensitive than the fathead minnow. Listed fish species were protected 96% of the time when results for both fathead minnows and C. dubia were considered, thus reinforcing the value of standard whole-effluent toxicity tests using those two species. If the responses of specific listed species are important for management decisions, our study supports the value in developing culture and testing procedures for those species. ?? 2005 Springer Science+Business Media, Inc.

Archives of Environmental Contamination and Toxico↗

Environmental contaminants in fish and their associated risk to piscivorous wildlife in the Yukon River Basin, Alaska

Organochlorine chemical residues and elemental contaminants were measured in northern pike (Esox lucius), longnose sucker (Catostomus catostomus), and burbot (Lota lota) from 10 sites in the Yukon River Basin (YRB) during 2002. Contaminant concentrations were compared to historical YRB data and to toxicity thresholds for fish and piscivorous wildlife from the scientific literature. A risk analysis was conducted to screen for potential hazards to piscivorous wildlife for contaminants that exceeded literature-based toxicity thresholds. Concentrations of total DDT (sum of p,p???-homologs; 1.09-13.6 ng/g), total chlordane (0.67-7.5 ng/g), dieldrin (<0.16-0.6 ng/g), toxaphene (<11-34 ng/g), total PCBs (<20-87 ng/g), TCDD-EQ (???1.7 pg/g), arsenic (0.03-1.95 ??g/g), cadmium (<0.02-0.12 ??g/g), copper (0.41-1.49 ??g/g), and lead (<0.21-0.27 ??g/g) did not exceed toxicity thresholds for growth and reproduction in YRB fish. Concentrations of mercury (0.08-0.65 ??g/g), selenium (0.23-0.85 ??g/g), and zinc (11-56 ??g/g) exceeded toxicity thresholds in one or more samples and were included in the risk analysis for piscivorous wildlife. No effect hazard concentrations (NEHCs) and low effect hazard concentrations (LEHCs), derived from literature-based toxicity reference values and avian and mammalian life history parameters, were calculated for mercury, selenium, and zinc. Mercury concentrations in YRB fish exceeded the NEHCs for all bird and small mammal models, which indicated that mercury concentrations in fish may represent a risk to piscivorous wildlife throughout the YRB. Low risk to piscivorous wildlife was associated with selenium and zinc concentrations in YRB fish. Selenium and zinc concentrations exceeded the NEHCs and LEHCs for only the small bird model. These results indicate that mercury should continue to be monitored and assessed in Alaskan fish and wildlife. ?? 2006 Springer Science+Business Media, Inc.

Archives of Environmental Contamination and Toxico↗

Mercury exposure and effects on cavity-nesting birds from the Carson River, Nevada

Mercury (Hg) concentrations were 15-40 times higher in the eggs and livers of tree swallows (Tachycineta bicolor) and house wrens (Troglodytes aedon) that nested along the Carson River at and below Dayton, Nevada than in the same species above the mining-impacted areas. Hg contamination was mainly the result of processing mills in the 1800s that used Hg to separate gold and silver from ore. The exposure pattern of tree swallows and house wrens along the Carson River was consistent with their trophic status (i.e., lower levels in liver tissue of aquatic insectivores than in piscivorous birds nesting nearby). Even though they are aquatic insectivores, tree swallows and house wrens were exposed to the same amount of Hg as piscivores in the Florida Everglades; this indicated the extreme level of Hg contamination in the Carson River. Only 70-74% of the eggs hatched. This was less than the nationwide average for these two species that generally hatch ???85% of eggs. Although the sample size was small, Hg might be impacting reproductive end points in cavity-nesting birds from the Carson River. Other trace elements were present at background concentrations. ?? 2006 Springer Science+Business Media, Inc.

Archives of Environmental Contamination and Toxico↗

Concentrations of metals in blood and feathers of nestling ospreys (Pandion haliaetus) in Chesapeake and Delaware Bays

In 2000, 2001, and 2002, blood and feather samples were collected from 40–45-day-old nestling ospreys ( Pandion haliaetus ) from Chesapeake Bay and Delaware Bay and River. Concentrations of 18 metals, metalloids, and other elements were determined in these samples by inductively coupled plasma-mass spectroscopy, and Hg concentrations were measured by cold vapor atomic absorption spectroscopy. When compared to concurrent reference areas (South, West, and Rhode Rivers), mean As and Hg concentrations in blood were greater ( p < 0.05) in two of three Chesapeake Bay regions of concern (Baltimore Harbor [As: 1.18 vs. 0.548 μg/g dw], Anacostia River [Hg: 0.305 vs. 0.178 μg/g dw], and Elizabeth River [As: 0.876 vs. 0.663 μg/g dw; Hg: 0.260 vs. 0.180 μg/g dw]). Lead was detected more frequently in blood of nestlings from the highly industrialized Elizabeth River compared to the rural reference area. When compared to the concurrent reference area, mean Al, Ba, Hg, Mn, and Pb concentrations in feathers were substantially greater ( p < 0.05) in one or more Chesapeake regions of concern (Anacostia River [Al: 206 vs. 62.1 μg/g dw; Ba: 3.31 vs. 0.823 μg/g dw; Mn: 65.4 vs. 22.9 μg/g dw] and Elizabeth River [Al: 165 vs. 63.5 μg/g dw; Hg: 1.24 vs. 0.599 μg/g dw; Pb 1.47 vs. 0.543 μg/g dw]). When compared to the coastal Inland Bays reference area, feathers of nestlings from northern Delaware Bay and River had greater concentrations ( p < 0.05) of Ba (1.90 vs. 0.660 μg/g dw), Fe (258 vs. 109 μg/g dw), Mn (18.5 vs. 4.66 μg/g dw), Mo (0.130 vs. 0.040 μg/g dw), Pb (1.96 vs. 0.624 μg/g dw), and V (0.671 vs. 0.325 μg/g dw), presumably due to extensive metal-working and petroleum refinery activities. Concentrations of Hg in nestling feathers from Delaware were frequently greater than in the Chesapeake. The present findings and those of related reproductive studies suggest that concentrations of several heavy metals (e.g., Cd, Hg, Pb) in nestling blood and feathers from Chesapeake and Delaware Bays were below toxicity thresholds and do not seem to be affecting chick survival during the nestling period.

Delaware, Maryland, New Jersey, Virginia↗

Element concentrations in tree swallows (Tachycineta bicolor) from the U.S. and Binational Great Lakes’ areas of concern

Selected elements were targeted in state Remedial Action Plans as one group of chemicals affecting the Beneficial Use Impairments of Great Lakes Areas of Concern (AOC). Livers of nestling tree swallows, Tachycineta bicolor, were harvested from 76 sites in the Great Lakes which included multiple sites at 27 AOCs and 12 reference sites from 2010 to 2015 and analyzed for 21 elements. Mercury concentrations were at background levels at all sites. Elevated cadmium (Cd) concentrations were associated with industry. The highest Cd values were from the Black River, OH AOC and associated with historic coke production, but were not at toxic levels. Lead (Pb) concentrations were highest on the Rouge River, MI AOC, the oldest and most heavily populated and industrialized area in southeast Michigan. Individual lead Pb concentrations were elevated to a level associated with delta-aminolevulinic acid dehydratase inhibition, but not to a level considered toxic. In contrast, livers harvested from sites on the southwest shore of Lake Michigan had selenium concentrations elevated to levels associated with reduced avian reproduction. One likely source of the high Se concentrations was pollution from a local coal-fired power plant. Concentrations of the remaining elements were at background levels.

Archives of Environmental Contamination and Toxico↗