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Biomedical and veterinary science can increase our understanding of coral disease

A balanced approach to coral disease investigation is critical for understanding the global decline of corals. Such an approach should involve the proper use of biomedical concepts, tools, and terminology to address confusion and promote clarity in the coral disease literature. Investigating disease in corals should follow a logical series of steps including identification of disease, systematic morphologic descriptions of lesions at the gross and cellular levels, measurement of health indices, and experiments to understand disease pathogenesis and the complex interactions between host, pathogen, and the environment. This model for disease investigation is widely accepted in the medical, veterinary and invertebrate pathology disciplines. We present standard biomedical rationale behind the detection, description, and naming of diseases and offer examples of the application of Koch's postulates to elucidate the etiology of some infectious diseases. Basic epidemiologic concepts are introduced to help investigators think systematically about the cause(s) of complex diseases. A major goal of disease investigation in corals and other organisms is to gather data that will enable the establishment of standardized case definitions to distinguish among diseases. Concepts and facts amassed from empirical studies over the centuries by medical and veterinary pathologists have standardized disease investigation and are invaluable to coral researchers because of the robust comparisons they enable; examples of these are given throughout this paper. Arguments over whether coral diseases are caused by primary versus opportunistic pathogens reflect the lack of data available to prove or refute such hypotheses and emphasize the need for coral disease investigations that focus on: characterizing the normal microbiota and physiology of the healthy host; defining ecological interactions within the microbial community associated with the host; and investigating host immunity, host-agent interactions, pathology, pathogenesis, and factors that promote the pathogenicity of the causative agent(s) of disease.

Journal of Experimental Marine Biology and Ecology↗

Accounting for spatiotemporal sampling variation in joint species distribution models

Estimating relative abundance is critical for informing conservation and management efforts and for making inferences about the effects of environmental change on populations. Freshwater fisheries span large geographic regions, occupy diverse habitats and consist of varying species assemblages. Monitoring schemes used to sample these diverse populations often result in populations being sampled at different times and under different environmental conditions. Varying sampling conditions can bias estimates of abundance when compared across time, location and species, and properly accounting for these biases is critical for making inferences. We develop a joint species distribution model (JSDM) that accounts for varying sampling conditions due to the environment and time of sampling when estimating relative abundance. The novelty of our JSDM is that we explicitly model sampling effort as the product of known quantities based on time and gear type and an unknown functional relationship to capture seasonal variation in species life history. We use the model to study relative abundance of six freshwater fish species across the state of Minnesota, USA. Our model enables estimates of relative abundance to be compared both within and across species and lakes, and captures the inconsistent sampling present in the data. We discuss how gear type, water temperature and day of the year impact catchability for each species at the lake level and throughout a year. We compare our estimates of relative abundance to those obtained from a model that assumes constant catchability to highlight important differences within and across lakes and species. Synthesis and applications : Our method illustrates that assumptions relating indices of abundance to observed catch data can greatly impact model inferences derived from JSDMs. Specifically, not accounting for varying sampling conditions can bias inference of relative abundance, restricting our ability to detect responses to management interventions and environmental change. While our focus is on freshwater fisheries, this model architecture can be adopted to other systems where catchability may vary as a function of space, time and species.

Journal of Applied Ecology↗

Dynamic social networks based on movement

Network modeling techniques provide a means for quantifying social structure in populations of individuals. Data used to define social connectivity are often expensive to collect and based on case-specific, ad hoc criteria. Moreover, in applications involving animal social networks, collection of these data is often opportunistic and can be invasive. Frequently, the social network of interest for a given population is closely related to the way individuals move. Thus, telemetry data, which are minimally invasive and relatively inexpensive to collect, present an alternative source of information. We develop a framework for using telemetry data to infer social relationships among animals. To achieve this, we propose a Bayesian hierarchical model with an underlying dynamic social network controlling movement of individuals via two mechanisms: an attractive effect and an aligning effect. We demonstrate the model and its ability to accurately identify complex social behavior in simulation, and apply our model to telemetry data arising from killer whales. Using auxiliary information about the study population, we investigate model validity and find the inferred dynamic social network is consistent with killer whale ecology and expert knowledge.

The Annals of Applied Statistics↗

Topobathymetric model of Mobile Bay, Alabama

Topobathymetric Digital Elevation Models (DEMs) are a merged rendering of both topography (land elevation) and bathymetry (water depth) that provides a seamless elevation product useful for inundation mapping, as well as for other earth science applications, such as the development of sediment-transport, sea-level rise, and storm-surge models. This 1/9-arc-second (approximately 3 meters) resolution model of Mobile Bay, Alabama was developed using multiple topographic and bathymetric datasets, collected on different dates. The topographic data were obtained primarily from the U.S. Geological Survey (USGS) National Elevation Dataset (NED) (http://ned.usgs.gov/) at 1/9-arc-second resolution; USGS Experimental Advanced Airborne Research Lidar (EAARL) data (2 meters) (http://pubs.usgs.gov/ds/400/); and topographic lidar data (2 meters) and Compact Hydrographic Airborne Rapid Total Survey (CHARTS) lidar data (2 meters) from the U.S. Army Corps of Engineers (USACE) (http://www.csc.noaa.gov/digitalcoast/data/coastallidar/). Bathymetry was derived from digital soundings obtained from the National Oceanic and Atmospheric Administration’s (NOAA) National Geophysical Data Center (NGDC) (http://www.ngdc.noaa.gov/mgg/geodas/geodas.html) and from water-penetrating lidar sources, such as EAARL and CHARTS. Mobile Bay is ecologically important as it is the fourth largest estuary in the United States. The Mobile and Tensaw Rivers drain into the bay at the northern end with the bay emptying into the Gulf of Mexico at the southern end. Dauphin Island (a barrier island) and the Fort Morgan Peninsula form the mouth of Mobile Bay. Mobile Bay is 31 miles (50 kilometers) long by a maximum width of 24 miles (39 kilometers) with a total area of 413 square miles (1,070 square kilometers). The vertical datum of the Mobile Bay topobathymetric model is the North American Vertical Datum of 1988 (NAVD 88). All the topographic datasets were originally referenced to NAVD 88 and no transformations were made to these input data. The NGDC hydrographic, multibeam, and trackline surveys were transformed from mean low water (MLW) or mean lower low water (MLLW) to NAVD 88 using VDatum (http://vdatum.noaa.gov). VDatum is a tool developed by the National Geodetic Survey (NGS) that performs transformations among tidal, ellipsoid-based, geoid-based, and orthometric datums using calibrated hydrodynamic models. The vertical accuracy of the input topographic data varied depending on the input source. Because the input elevation data were derived primarily from lidar, the vertical accuracy ranges from 6 to 20 centimeters in root mean square error (RMSE). he horizontal datum of the Mobile Bay topobathymetric model is the North American Datum of 1983 (NAD 83), geographic coordinates. All the topographic and bathymetric datasets were originally referenced to NAD 83, and no transformations were made to the input data. The bathymetric surveys were downloaded referenced to NAD 83 geographic, and therefore no horizontal transformations were required. The topbathymetric model of Mobile Bay and detailed metadata can be obtained from the USGS Web sites: http://nationalmap.gov/.

Alabama↗

Time to branch out? Application of hierarchical survival models in plant phenology

The sensitivity of phenology to environmental drivers can vary across geography and species. As such, models developed to predict phenology are typically site- or taxon-specific. Generation of site- and taxon-specific models is limited by the intensive in-situ phenological monitoring effort required to generate sufficient data to parameterize each model. Where in-situ phenological observations exist, the data are often subject to analytical issues due to the limited duration of any individual monitoring program, spotty site- and species- level coverage, lack of standardized methodology, and infrequent or variable census intervals. Together, these characteristics constrain our ability to make phenological inferences outside of select sites and taxa where long-duration, intensive monitoring has occurred. In this study, we leveraged two national, standardized phenology datasets to develop a multi-species and multi-site state-space survival model of the onset of deciduous tree and shrub spring (leaf out) and fall (leaf-color) events across temperate ecoregions of the United States. We used data from two national-scale phenological databases, a 9-year, broadly distributed dataset from the USA National Phenology Network and a 4-year dataset from the National Ecological Observatory Network, to quantify regional and interspecific variation in sensitivity to environmental drivers for both spring and fall leaf phenophases. Spring leaf out was generally promoted by longer days, spring growing degree day accumulation, overwinter chilling, and was suppressed by frost events, whereas fall leaf color was promoted by shorter days and cold accumulation. The sensitivity to most environmental drivers tended to be more variable among species than among the regions as defined here (EPA ecoregions of North America, excluding desert and tropical areas). The results of this study lay the groundwork for incorporating the growing collection of phenological observations into a generalized framework for predicting the transition states for any species, in any location.

Agricultural and Forest Meteorology↗

Effects of insecticide spray drift on arthropod prey resources of birds in grasslands in Minnesota

Soybean aphid ( Aphis glycines ) insecticides are used throughout the Upper Midwest and Great Plains regions of North America, including the farmland region of Minnesota, USA, to combat insect pests. These broad-spectrum, foliar spray insecticides have the potential to drift beyond target fields into nearby grassland cover where birds and other insectivores forage. Arthropods serve important roles in grassland ecology and are susceptible to mortality and sublethal effects from exposure to these pesticides. Our objective was to assess effects of soybean aphid insecticides on grassland arthropods, especially those that are important in grassland bird diets. We measured the abundance, consumable biomass, and family richness of insects and spiders in grasslands adjacent to soybean fields in an agricultural landscape. Soybean fields were treated with chlorpyrifos and lambda-cyhalothrin, which were the 2 most common foliar pesticides used to control soybean aphids in Minnesota. We compared measures at focal sites to samples collected at reference sites adjacent to corn fields not sprayed for aphids during 3 periods in mid-to-late summer: 1–3 days before spraying, 3–5 days post-spraying, and 19–21 days post-spraying. The abundance of arthropods in focal grasslands was lower 3–5 days after pesticide applications. Coleoptera family richness at focal sites was also lower than at reference sites 3–5 days after pesticide applications. These measures 19–21 days after application were similar to pre-spraying levels, indicating that arthropod populations rebounded during this period. Measures of consumable dry biomass, bird prey abundance, bird prey biomass, family richness of Araneae, family richness of Hemiptera, and family richness of Orthoptera were not different between focal and reference sites after spraying. Our results reveal that reductions in arthropod food abundance for grassland birds are associated with pesticide applications up to 5 days after spraying. We suggest that natural resource managers factor proximity to row crop fields and susceptibility to pesticide drift into decisions about where to add grassland cover to landscapes.

Minnesota↗

Aquatic pollution increases use of terrestrial prey subsidies by stream fish

Stream food webs are connected with their riparian zones through cross-ecosystem movements of energy and nutrients. The use and impact of terrestrial subsidies on aquatic consumers is determined in part by in situ biomass of aquatic prey. Thus, stressors such as aquatic pollutants that greatly reduce aquatic secondary production could increase the need for and reliance of stream consumers on terrestrial resource subsidies. To test this hypothesis, we surveyed stream fish, their diets and resource availability in 16 subalpine streams over a regional gradient of trace metals known to strongly impact aquatic insect communities (i.e. fish prey) in the Colorado Rocky Mountains, USA. Fish increased their reliance on terrestrial insect prey as stream metals increased. Relative biomass of terrestrial insects in stomach contents of Brook and Brown Trout increased with respect to aquatic insect biomass and total stomach contents. Drifting insect biomass showed a declining trend for aquatic, but not terrestrial insects, over the metal gradient. Trout densities were unrelated to metal concentrations in streams where we found fish. Synthesis and applications . Our results indicate that diets of aquatic consumers can become more terrestrial as aquatic stressors that limit in situ food production increase and that these subsidies may compensate for loss of aquatic resources. This work implies an important connection between preserving aquatic–terrestrial linkages and management of fish populations in stressed watersheds. Specifically, intact riparian zones and aquatic–terrestrial linkages are likely to be important for maintaining trout production in streams with moderate metal contamination.

Colorado↗

Using a full annual cycle model to evaluate long-term population viability of the conservation-reliant Kirtland's warbler after successful recovery

Long-term management planning for conservation-reliant migratory songbirds is particularly challenging because habitat quality in different stages and geographic locations of the annual cycle can have direct and carry-over effects that influence the population dynamics. The Neotropical migratory songbird Kirtland's warbler Setophaga kirtlandii (Baird 1852) is listed as endangered under the U.S. Endangered Species Act and Near Threatened under the IUCN Red List. This conservation-reliant species is being considered for U.S. federal delisting because the species has surpassed the designated 1000 breeding pairs recovery threshold since 2001. To help inform the delisting decision and long-term management efforts, we developed a population simulation model for the Kirtland's warbler that incorporated both breeding and wintering grounds habitat dynamics, and projected population viability based on current environmental conditions and potential future management scenarios. Future management scenarios included the continuation of current management conditions, reduced productivity and carrying capacity due to the changes in habitat suitability from the creation of experimental jack pine Pinus banksiana (Lamb.) plantations, and reduced productivity from alteration of the brown-headed cowbird Molothrus ater (Boddaert 1783) removal programme. Linking wintering grounds precipitation to productivity improved the accuracy of the model for replicating past observed population dynamics. Our future simulations indicate that the Kirtland's warbler population is stable under two potential future management scenarios: (i) continuation of current management practices and (ii) spatially restricting cowbird removal to the core breeding area, assuming that cowbirds reduce productivity in the remaining patches by ≤41%. The additional future management scenarios we assessed resulted in population declines. Synthesis and applications . Our study indicates that the Kirtland's warbler population is stable under current management conditions and that the jack pine plantation and cowbird removal programmes continue to be necessary for the long-term persistence of the species. This study represents one of the first attempts to incorporate full annual cycle dynamics into a population viability analysis for a migratory bird, and our results indicate that incorporating wintering grounds dynamics improved the model performance.

Michigan↗

Nest survival modelling using a multi-species approach in forests managed for timber and biofuel feedstock

Switchgrass ( Panicum virgatum ) intercropping is a novel forest management practice for biomass production intended to generate cellulosic feedstocks within intensively managed loblolly pine‐dominated landscapes. These pine plantations are important for early‐successional bird species, as short rotation times continually maintain early‐successional habitat. We tested the efficacy of using community models compared to individual surrogate species models in understanding influences on nest survival. We analysed nest data to test for differences in habitat use for 14 bird species in plots managed for switchgrass intercropping and controls within loblolly pine ( Pinus taeda ) plantations in Mississippi, USA. We adapted hierarchical models using hyper‐parameters to incorporate information from both common and rare species to understand community‐level nest survival. This approach incorporates rare species that are often discarded due to low sample sizes, but can inform community‐level demographic parameter estimates. We illustrate use of this approach in generating both species‐level and community‐wide estimates of daily survival rates for songbird nests. We were able to include rare species with low sample size (minimum n = 5) to inform a hyper‐prior, allowing us to estimate effects of covariates on daily survival at the community level, then compare this with a single‐species approach using surrogate species. Using single‐species models, we were unable to generate estimates below a sample size of 21 nests per species. Community model species‐level survival and parameter estimates were similar to those generated by five single‐species models, with improved precision in community model parameters. Covariates of nest placement indicated that switchgrass at the nest site (<4 m) reduced daily nest survival, although intercropping at the forest stand level increased daily nest survival. Synthesis and applications . Community models represent a viable method for estimating community nest survival rates and effects of covariates while incorporating limited data for rarely detected species. Intercropping switchgrass in loblolly pine plantations slightly increased daily nest survival at the research plot scale (0.1 km 2 ), although at a local scale (50 m 2 ) switchgrass negatively influenced nest survival. A likely explanation is intercropping shifted community composition, favouring species with greater disturbance tolerance.

Journal of Applied Ecology↗

Determinants of Pseudogymnoascus destructans within bat hibernacula: Implications for surveillance and management of white-nose syndrome

Fungal diseases are an emerging global problem affecting human health, food security and biodiversity. Ability of many fungal pathogens to persist within environmental reservoirs can increase extinction risks for host species and presents challenges for disease control. Understanding factors that regulate pathogen spread and persistence in these reservoirs is critical for effective disease management. White-nose syndrome (WNS) is a disease of hibernating bats caused by Pseudogymnoascus destructans ( Pd ), a fungus that establishes persistent environmental reservoirs within bat hibernacula, which contribute to seasonal disease transmission dynamics in bats. However, host and environmental factors influencing distribution of Pd within these reservoirs are unknown. We used model selection on longitudinally collected field data to test multiple hypotheses describing presence–absence and abundance of Pd in environmental substrates and on bats within hibernacula at different stages of WNS. First detection of Pd in the environment lagged up to 1 year after first detection on bats within that hibernaculum. Once detected, the probability of detecting Pd within environmental samples from a hibernaculum increased over time and was higher in sediment compared to wall surfaces. Temperature had marginal effects on the distribution of Pd . For bats, prevalence and abundance of Pd were highest on Myotis lucifugus and on bats with visible signs of WNS. Synthesis and applications . Our results indicate that distribution of Pseudogymnoascus destructans ( Pd ) within a hibernaculum is driven primarily by bats with delayed establishment of environmental reservoirs. Thus, collection of samples from Myotis lucifugus , or from sediment if bats cannot be sampled, should be prioritized to improve detection probabilities for Pd surveillance. Long-term persistence of Pd in sediment suggests that disease management for white-nose syndrome should address risks of sustained transmission from environmental reservoirs.

Journal of Applied Ecology↗

Applying the ecology of aquatic–terrestrial linkages to freshwater and riparian management

Global stressors such as climate change, invasive species, urbanization, agricultural practices, and pollution can alter aquatic resource subsidies to terrestrial consumers. The effects of these stressors on timing, quality, and quantity of aquatic subsidies, such as adult aquatic insects, to birds, herpetofauna, and mammals, have large implications for wildlife management (Baxter et al. 2004 , Saunders and Fausch 2007 , Walters et al. 2008 , Sullivan and Rodewald 2012 , Morrissey et al. 2015 , Kraus et al. 2016 , Larsen et al. 2016 , Sullivan et al. 2019 ). For example, insect-mediated contaminant transport from polluted rivers expose song bird nestlings and other protected birds to potentially toxic levels of persistent organic contaminants and pharmaceuticals (Walters et al. 2010 , Richmond et al. 2018 ). Recent declines in aquatic insect production caused by pollution and changes in land use have been tied to global declines in terrestrial insectivores such as birds and bats (Hallmann et al. 2014 , Morrissey et al. 2015 , Raby et al. 2018 ). These natural-resource impacts from aquatic–terrestrial exposure and loss of resource subsidies are leading to changes in monitoring protocols and how management agencies evaluate the effectiveness of corrective remedies (Muehlbauer et al. 2019 ). For researchers interested in the application of resource subsidy research, a logical next step is to help practitioners anticipate effects of global stressors on aquatic-terrestrial linkages and incorporate these principles into decision making.

Freshwater Science↗

Measuring and evaluating ecological flows from streams to regions: Steps towards national coverage

Living aquatic communities are largely determined and maintained by the volume and quality of flowing waters, both within lotic systems and in receiving waters of coastal systems. However, flow is one of the most frequently and extensively altered features of rivers and streams; alteration effects are likely to be exacerbated by climate change. Lotic systems vary and different fish species need different environmental conditions, and distinct problems are evident at various spatial scales. New synoptic flow and biological information now make it possible to evaluate the effects of altered flows throughout the Great Lakes Region at scales from the stream reach to the Region. We used estimates of river and streamflow and observed fish abundances to develop tools that specify the response of fish to alterations in those flows. We fit the logistic model to a cumulative fish abundance curve as a function of yield providing an empirical means to develop models of the response of cumulative fish abundance to flows. Response zones of yield for each species in each system type (based on size and thermal class) illustrate how criteria may be developed that can be used in decision‐making for management of flows. In our example application, we evaluate both the general response of brook trout ( Salvelinus fontinalis ) abundances (and fish diversity) to changes in flows and assess the sensitivity of each stream fish community to flow alteration. Mapping stream sensitivity to flow alteration throughout the US Great Lakes Region with a multiscale spatial framework showed how regional variability in sensitivity for any fish species or assemblage may be evaluated and provides managers with information to help determine where the best opportunities for protection or restoration of streamflows and associated communities exist. These results provide valuable tools and critical information to managers responsible for balancing water uses and maintaining high quality lotic ecosystems. These methods may be applied to any geographic region and can be extended nationally or globally, where flow, temperature, fish and landscape data are available.

Illinois, Indiana, Michigan, Minnesota, New York, ↗

Pit tag application in native freshwater mussels: Case studies across small, medium, and large rivers

Since their first use in the mid-1980s, external passive integrated transponder (PIT) tags have facilitated innovative investigations into multiple biological traits of animals. For native freshwater mussels, PIT tags are frequently used in capture-mark-recapture applications because they allow repeated, noninvasive sampling, are easy to apply, have high retention rates, and have negligible short-term effects on growth and survival. Because of these traits, resource managers and scientists are using PIT-tagged animals to estimate survival and movement of mussels associated with restoration efforts. However, consistency is limited in how PIT tags are affixed, monitored, and reported. Thus, our objectives were to (1) share our collective experiences in PIT tagging mussels across three case studies in small, medium, and large rivers and (2) propose guidelines for tagging and reporting data from PIT tag studies with native freshwater mussels to facilitate comparisons across future studies. The number of studies that have marked mussels with PIT tags has increased over the past 10 years. The ability to detect mussels using PIT tags has substantially advanced research in three areas of mussel ecology: (1) estimating vital rates (e.g., growth and survival), (2) tracking movements and behaviors of captively propagated, wild, and translocated individuals, and (3) improving our understanding of life history traits, such as reproductive timing. Each case study offers insights on tagging methods, tag loss, tag retention, and monitoring frequency across multiple species that range in conservation status from common to rare. We conclude with best-practice guidelines for placing PIT tags on freshwater mussels and a list of variables that could be reported in future studies to facilitate cross-system comparisons.

Freshwater Mollusk Biology and Conservation↗

Using mobile acoustic monitoring and false-positive N-mixture models to estimate bat abundance and population trends

Estimating the abundance of unmarked animal populations from acoustic data is challenging due to the inability to identify individuals and the need to adjust for observation biases including detectability (false negatives), species misclassification (false positives), and sampling exposure. Acoustic surveys conducted along mobile transects were designed to avoid counting individuals more than once, where raw counts are commonly treated as an index of abundance. More recently, false-positive abundance models have been developed to estimate abundance while accounting for imperfect detection and misclassification. We adapted these methods to model summertime abundance and trends of three species of bats at multiple spatial scales using acoustic recordings collected along mobile transects by partners of the North American Bat Monitoring Program (NABat) from 2012 to 2020. This multiscale modeling spanned individual transect routes, larger NABat grid cells (10 km × 10 km), and across the entire extent of modeled species ranges. We estimated relationships between species abundances and a suite of abiotic and biotic predictors (landcover types, climatological variables, physiographic diversity, building density, and the impacts of white-nose syndrome [WNS]) and found varying levels of support between species. We present clear evidence of substantial declines in populations of tricolored bats ( Perimyotis subflavus ) and little brown bats ( Myotis lucifugus ), declines that corresponded in space and time with the progression of WNS, a devastating disease of hibernating bats. In contrast, our analysis revealed that similar population-wide declines probably have not occurred in big brown bats ( Eptesicus fuscus ), a species known to be less affected by WNS. This study provides the first abundance-based species distribution predictions and population trends for bats in their summer ranges in North America. These models will probably be applicable to assessing wildlife populations in other monitoring programs where acoustic data are used or where false-negative and false-positive detections are present. Finally, our abundance framework (as a spatial point pattern process) can serve as a foundation from which more sophisticated integrated species distribution models that incorporate additional streams of monitoring data (e.g., stationary acoustics, captures) can be developed for North American bats.

Ecological Monographs↗

Accounting for false-positive acoustic detections of bats using occupancy models

1. Acoustic surveys have become a common survey method for bats and other vocal taxa. Previous work shows that bat echolocation may be misidentified, but common analytic methods, such as occupancy models, assume that misidentifications do not occur. Unless rare, such misidentifications could lead to incorrect inferences with significant management implications. 2. We fit a false-positive occupancy model to data from paired bat detector and mist-net surveys to estimate probability of presence when survey data may include false positives. We compared estimated occupancy and detection rates to those obtained from a standard occupancy model. We also derived a formula to estimate the probability that bats were present at a site given its detection history. As an example, we analysed survey data for little brown bats Myotis lucifugus from 135 sites in Washington and Oregon, USA. 3. We estimated that at an unoccupied site, acoustic surveys had a 14% chance per night of producing spurious M. lucifugus detections. Estimated detection rates were higher and occupancy rates were lower under the false-positive model, relative to a standard occupancy model. Un-modelled false positives also affected inferences about occupancy at individual sites. For example, probability of occupancy at individual sites with acoustic detections but no captures ranged from 2% to 100% under the false-positive occupancy model, but was always 100% under a standard occupancy model. 4. Synthesis and applications. Our results suggest that false positives sufficient to affect inferences may be common in acoustic surveys for bats. We demonstrate an approach that can estimate occupancy, regardless of the false-positive rate, when acoustic surveys are paired with capture surveys. Applications of this approach include monitoring the spread of White-Nose Syndrome, estimating the impact of climate change and informing conservation listing decisions. We calculate a site-specific probability of occupancy, conditional on survey results, which could inform local permitting decisions, such as for wind energy projects. More generally, the magnitude of false positives suggests that false-positive occupancy models can improve accuracy in research and monitoring of bats and provide wildlife managers with more reliable information.

Oregon, Washington↗

Using conservation value to assess land restoration and management alternatives across a degraded oak savanna landscape

1. Managers considering restoration of landscapes often face a fundamental challenge - what should be the habitat composition of the restored landscape? We present a method for evaluating an important conservation trade-off inherent in making that decision. 2. Oak savannas and grasslands were historically widespread across central North America but are now rare. Today, in north-west Indiana, USA, habitats spanning a range of woody vegetation density, from nearly treeless open habitats to forests, occur across the conserved landscape where savannas probably once dominated. To understand the benefits of different potential landscape compositions, we evaluated how different proportions of five habitats - open, savanna, woodland, scrub and forest - might affect the conservation value of the north-west Indiana landscape for birds. Two variables of potential conservation importance were examined: species diversity, a measure of avian community richness, and conservation index, the percentage of a bird species' global population occurring on a hectare of landscape, summed across all bird species present. Higher values of conservation index were associated with higher local densities of globally more rare and more threatened species. 3. Conservation index and species diversity were correlated negatively across hypothetical landscapes composed of different proportions of the five habitats. Therefore, a management trade-off existed between conservation index and species diversity because landscapes that maximized species diversity differed from landscapes that maximized conservation index. 4. A landscape of 50% open, 22% savanna, 15% scrub and 13% forest was predicted to represent a compromise at which conservation index and species diversity reached the same percentage of their maxima. In contrast, the current landscape is dominated by forest. 5. Synthesis and applications. We quantified the trade-off between two potential aspects of a landscape's conservation value for birds - the landscape's ability to promote avian species diversity and the landscape's use by threatened avian species. This quantification allowed us to evaluate the ability of different landscape compositions to achieve preferable trade-off compromises, such as maximizing diversity for a given level of landscape use by threatened species. Managers can use these trade-off results to determine which landscape compositions are associated with particular conservation and management priorities.

Journal of Applied Ecology↗

Water quality modeling in the systems impact assessment model for the Klamath River basin - Keno, Oregon to Seiad Valley, California

This report describes the water quality model developed for the Klamath River System Impact Assessment Model (SIAM). The Klamath River SIAM is a decision support system developed by the authors and other US Geological Survey (USGS), Midcontinent Ecological Science Center staff to study the effects of basin-wide water management decisions on anadromous fish in the Klamath River. The Army Corps of Engineersa?? HEC5Q water quality modeling software was used to simulate water temperature, dissolved oxygen and conductivity in 100 miles of the Klamath River Basin in Oregon and California. The water quality model simulated three reservoirs and the mainstem Klamath River influenced by the Shasta and Scott River tributaries. Model development, calibration and two validation exercises are described as well as the integration of the water quality model into the SIAM decision support system software. Within SIAM, data are exchanged between the water quantity model (MODSIM), the water quality model (HEC5Q), the salmon population model (SALMOD) and methods for evaluating ecosystem health. The overall predictive ability of the water quality model is described in the context of calibration and validation error statistics. Applications of SIAM and the water quality model are described.

California, Oregon↗

Hyperspectral Image Transects during Transient Events in Rivers (HITTER): Framework development and application to a tracer experiment on the Missouri River, USA

Rivers convey a broad range of materials, such as sediment, nutrients, and contaminants. Much of this transport can occur during or immediately after an episodic, pulsed event like a flood or an oil spill. Understanding the flow processes that influence the motion of these substances is important for managing water resources and conserving aquatic ecosystems. This study introduces a new remote sensing framework for characterizing dynamic phenomena at the scale of a channel cross-section: Hyperspectral Image Transects during Transient Events in Rivers (HITTER). We present a workflow that uses repeated hyperspectral scan lines acquired from a hovering uncrewed aircraft system (UAS) to quantify how a water attribute of interest varies laterally across the river and evolves over time. Data from a tracer experiment on the Missouri River are used to illustrate the components of the end-to-end processing chain we used to quantify the passage of a visible dye. The framework is intended to be flexible and could be applied in a number of different contexts. The results of this initial proof-of-concept investigation suggest that HITTER could potentially provide insight regarding the dispersion of a range of materials in rivers, which would facilitate ecological and geomorphic studies and help inform management.

Remote Sensing↗