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At least 1,657 records · Page 92Linked to original sources

Early Pliocene (Zanclean) sea surface temperature for PlioMIP3

Paleoclimate researchers have been comparing Pliocene environmental data to paleoclimate model results since the 1980s. The Pliocene Model Intercomparison Project (PlioMIP) began in 2008 with a focus on the Late Pliocene. Here we assess the availability and utility of sea surface temperature (SST) data for verification of Pliocene Model Intercomparison Project (PlioMIP3) Early Pliocene (Zanclean) experiments. We analyze published data in terms of quantity and spatial distribution. Only SST estimates derived using alkenone paleo thermometry are reported, and all estimates are based upon the same temperature calibration. Sea surface temperature data are selected from within three distinct time intervals: The early Zanclean 5.3 Ma – 4.2 Ma time slab, and two time slices within the early Zanclean, chosen by PlioMIP3 at 4.870 Ma and 4.474 Ma. Results show the early Zanclean time slab contains 2055 individual estimates. Approximately ∼ 80% of these estimates come from Sites 609, 642, 846, 847, 882, 907, and 1146. There are 17 sites with a total of 42 estimates within the 4.474 Ma ±10 kyr time slice, and 15 sites with a total of 47 data points within the 4.870 Ma ±10 kyr interval. The sparse spatial and temporal distribution of Zanclean data, relative to the data available for the mid Piacenzian, makes point-by-point data model comparison suspect. We suggest interpreting model output against lower resolution long term trends in proxy data, and comparison of models through temperature gradients, may be the most useful application of currently available data. Integrating Zanclean age coastal plain sequences within data model comparison schemes, for increased understanding of regional climate impacts, also holds great potential.

Global and Planetary Change↗

Limitations and potential applicability of mixing models and transit time estimates for mine-affected watersheds based on stable isotopes

Stable isotopes are a widely used tool in defining mixing of distinct waters and identifying solute sources on mine sites. Although the utility of stable isotopes in these approaches is well-acknowledged, analytical uncertainties and lack of endmember definition may limit the application of results in providing unambiguous information to facilitate remediation. In this context, this study used a multi-year high-frequency (monthly to weekly) dataset of δ 2 H, δ 18 O, and δ 34 S collected from streams surrounding a mine site with a draining adit, the Captain Jack Mill superfund site near Boulder, Colorado, USA, to evaluate mixing between multiple distinctive potential solute and water sources. Iterative endmember mixing analysis was conducted to evaluate if stable isotopes can discriminate between mine water that is evaporatively fractionated in a surface pond versus diffuse discharge of mine water to the stream through the streambed. Timeseries analysis of paired estimated precipitation and stream isotopes was also conducted to determine if mine water discharge affects watershed-scale transit time metrics. Results indicate that model uncertainty propagated from analytical uncertainty overwhelms endmember mixing results using δ 2 H and δ 18 O because all water on the site is sourced from meteoric precipitation, limiting the definition of distinct endmembers. In contrast, endmember mixing results using δ 34 S are highly sensitive to the simulated mixing fractions and results indicate that approximately half of the sulfate in the downstream stream water is derived from the mine adit and half is sourced from upstream watershed sources such as surface mine waste piles or diffuse groundwater discharge. Transit time metrics are statistically indistinguishable from upstream to downstream of the mine, meaning that mine water discharge does not cause a shift in transit times at the watershed scale. This study outlines notable limitations in the application of stable isotopes for informing remedial decisions at mine sites.

Colorado↗

Revisiting chlorophyll a thresholds for San Francisco Bay: Insights from observations of phytoplankton molecular abundance

Harmful Algal Blooms (HABs) are a hazard for coastal environments worldwide; identifying screening thresholds of chlorophyll- a (chl- a) associated with increased risk of HABs is a management priority. Molecular surveillance of coastal phytoplankton and bivalve biotoxins could be used to link chl- a with HAB risk, but requires an understanding of whether the HAB risks increase uniformly as chl-a rises, or whether some taxa are disproportionately favored, and if these relationships vary by season. In this study, we present a novel use of molecular abundance data to investigate the scientific bases for estuarine chl- a thresholds protective against HABs. In San Francisco Bay (SFB), California, the relationship between molecular relative abundance (as measured by 18S metabarcoding) of nine different HAB taxa, absolute quantitative polymerase chain reaction (qPCR) abundance, and mussel toxin concentrations of a subset of the taxa were investigated for thresholds as a function of increasing chl- a . Our results show most HAB taxa did not increase in absolute or relative abundance during SFB’s spring bloom interval, when chl- a levels were highest (>10 µg/L) but the assemblage was dominated by non-harmful diatoms. However, several flagellated, mixotrophic taxa did increase above their molecular baseline in fall, and the combined probability of any HAB occurring above baseline was elevated when chl- a reached ∼4.6 µg/L in the fall. This work demonstrates the promise of molecular approaches in disentangling the seasonally complex interplay between stressors and phytoplankton/HAB community responses and has the potential to provide clearer, more cost-effective monitoring and mitigation strategies for managers.

California↗

Comparative evaluation of three extraction techniques: Validation of a novel approach for the determination of 51 Per- and Polyfluoroalkyl substances in Graminaceous plants

Per- and polyfluoroalkyl substances (PFAS) are a class of over 15,000 xenobiotic compounds that have permeated across the globe and are largely resistant to natural forms of degradation, resulting in their long-term environmental persistence. These compounds can bioaccumulate and cause toxicological effects in organisms living within PFAS contaminated ecosystems. Grasses and feedstocks grown on contaminated farmlands are intermediates in the transmission of PFAS from contaminated soil to livestock and humans and may provide a potential avenue for biomonitoring of PFAS as they permeate through agricultural food systems. However, extraction methods for these compounds in graminaceous plants are sparse, non-standardized and limited in analytical range of PFAS compounds. This study outlines the development of a novel method of extraction using an alkaline, methanol-based solid-liquid extraction technique for 51 PFAS in graminaceous plant material, including nine separate PFAS subclasses. In comparison to two other previously published methods, this alkaline, methanolic solid-liquid approach has statistically significantly improved analyte recoveries for 22 of the 51 tested PFAS, ranging from 64.7–139% recovery in previously unexposed prairie grass ( Panicum virgatum and Dactylis glomerata ). Method detection limits range from 0.091 – 53.7 parts per trillion across tested PFAS analytes, demonstrating increased precision and sensitivity when compared to two other tested extraction procedures. This method’s strategy of alkaline, methanolic SLE may serve as a foundation for further testing across new plant taxa and an increased range of PFAS analytes.

Journal of Hazardous Materials Advances↗

Downstream persistence of cyanobacteria in New Jersey's Raritan River basin

Few studies have examined cyanobacteria persistence starting from lacustrine cyanobacteria harmful algal blooms (HABs) downstream. Multiple lakes and reservoirs within New Jersey's Raritan Basin Water Supply Complex (RBWSC) feature recurrent HABs and discharge water into the Raritan River. As the RBWSC provides drinking water to 1.5 million people, these HABs create drinking water treatment and human health concerns. This study quantitatively reviews the persistence of cyanobacteria communities across the RBWSC from cyanobacteria-dominated lacustrine sources to downstream drinking water and qualitatively assess what, if any, water chemistry parameters influenced the persistence, growth, or decline of cyanobacteria during downstream transport. Statistical tests showed there was a lack of continuity between cyanobacteria communities along the Raritan River, except for periods of higher streamflow and higher releases from the Spruce Run Reservoir. Results along the Millstone River suggested the potential for in-river cyanobacteria growth, which overlapped with periods of low streamflow and high nutrient concentrations in the summer and fall. A permutational analysis of variance (PERMANVOA) test found significantly dissimilar ( p < 0.01) cyanobacteria communities between lake and reservoir outlet sites and downstream sites in the Raritan, but not the Millstone River ( p = 0.928). A similarity percentage (SIMPER) analysis found cyanobacteria communities changed from high densities of bloom forming cyanobacteria ( Aphanocapsa, Aphanizomenon, Microcystis) at the lake outlet sites to low densities of non-bloom forming cyanobacteria ( Synechcoccus, Chroococcus) along the Raritan mainstem, with Aphanocapsa the most notable bloom forming genera overlapping between sites. Investigating cyanobacteria on a watershed-specific basis could enhance understanding of the site-specific transport mechanisms and water chemistry parameters that facilitate community persistence.

New Jersey↗

Mars as one system: Insights from the 8th international conference on Mars polar science and exploration

The 8th International Conference on Mars Polar Science and Exploration (ICMPSE) provided new insights into the complex interplay between Martian ice, climate, and atmospheric dynamics. The conference emphasized a holistic view of Mars as a system, where surface processes, subsurface ice, atmospheric dynamics, and historical climate shifts are interconnected. Research updates spanned cutting-edge remote sensing techniques and observations, terrestrial analog studies, and theoretical modeling, providing a multidimensional view of Mars' dynamic history and present-day activity. Numerous presentations highlighted the importance of understanding Mars as an integrated and dynamic system where surface and atmospheric processes interact over various timescales. This paper synthesizes unresolved questions and the major themes discussed, including mid-latitude glaciation, the evolution of the polar layered deposits (PLDs), terrestrial analog studies, climate modeling, and future mission planning. The findings contributed to refining models of Martian climate history, assessing the potential for extant life, and planning future human exploration. We also discuss the historical perspective of Mars Polar Science and new findings since the 7th conference in this series and anticipate the next four to 20 years of science on Mars related to ice, climate, and the atmosphere.

Icarus↗

Lunar analog study using portable gamma-ray neutron detector: Radiochemical mapping of silicic-to-basaltic volcanic terrains in the San Francisco Volcanic Field, Arizona

Studying lunar silicic volcanism provides key insights into the Moon’s crustal evolution, magmatic processes, and volcanic history. The Lunar-VISE (Lunar Vulkan Imaging and Spectroscopy Explorer) mission will investigate the Gruithuisen domes, a unique lunar region hypothesized to have formed through silicic volcanism. Using instruments on a Firefly Aerospace lander and a Honeybee Robotics rover, Lunar-VISE will analyze mineralogy, geochemistry, and surface properties to determine the origin and evolution of the domes, with a gamma-ray and neutron spectrometer (LV-GRNS) among its payload instruments. In preparation for this mission, we conducted preliminary fieldwork using a handheld gamma-ray neutron detector with NaI(Tl) and Cs LiYCl :Ce (CLYC) scintillators. This study focuses on various rhyolitic and basaltic volcanic centers in the San Francisco Volcanic Field (SFVF) near Flagstaff, Arizona—specifically Sugarloaf Peak, Bonito Lava Flow, and Robinson Mountain, a region containing several well-characterized lunar analog sites. The SFVF was selected for this study due to its broad compositional diversity spanning basaltic to rhyolitic compositions, its well-preserved volcanic morphologies, and its extensive use in previous NASA field campaigns and astronaut training exercises, making it an ideal terrestrial laboratory for testing planetary exploration techniques. We measured natural radioactivity, specifically from potassium (K), thorium (Th), and uranium (U) and other elements in their decay chains, which serve as diagnostic tracers of magmatic differentiation and crustal evolution processes, to assess geochemical variability across compositionally diverse terrains. The detector’s sensitivity was assessed across varying concentration levels. Regionally averaged concentrations of radioisotopes were determined by gamma-ray spectroscopy at selected sites. Our measurements reveal significant compositional variations between sites, with Sugarloaf Peak (rhyolitic, silicic dome) exhibiting the highest average radioisotope concentrations (K: 3.29 ± 0.24 wt%, U: 14.35 ± 1.81 ppm, Th: 27.14 ± 2.43 ppm), while Bonito Lava Flow (K: 1.13 ± 0.08 wt%, U: 3.86 ± 0.52 ppm, Th: 7.52 ± 1.02 ppm), and Robinson Mountain (K: 1.46 ± 0.16 wt%, U: 5.59 ± 1.06 ppm, Th: 11.75 ± 1.98 ppm) show lower concentrations aligned with basaltic compositions. Gamma-ray fluxes were elevated by a factor of approximately three to four at Sugarloaf Peak relative to nearby basaltic terrains, consistent with expected geochemical differentiation patterns. Furthermore, at meter scales, proximity to geological features significantly affects measurements. Th concentrations adjacent to a cliff face at Sugarloaf’s base were 28% higher than values measured 3-5 meters away from the same feature, demonstrating localized compositional heterogeneity. Analysis of station-by-station measurements reveals that K, U, and Th concentrations show an elevation trend at Robinson Mountain and generally higher concentrations near Sugarloaf Peak’s summit, suggesting progressive magmatic differentiation and/or the presence of more evolved lithologies at higher elevations. By mapping these elements on Earth using GRNS, we aim to optimize measurement techniques for Lunar-VISE and similar rover-borne missions by demonstrating the applicability of portable GRNS instruments for planetary surface exploration in an analog environment.

Arizona↗

Amazonian mass balance history of Martian ice reservoirs: Current knowledge and uncertainties

A major part of the history of the Amazonian period on Mars is the movement of water ice between near-surface reservoirs across the planet. This work assesses the ages and volumes of different parts of the Martian water ice inventory over the last ∼1 Gyr, to reconstruct the time history of ice reservoirs to the extent that it is currently known and determine whether they provide a mass-balanced history. We find that the available constraints do not provide a mass-balanced history over the last 1 Gyr: published age and volume estimates for past ice are unable to account for the former location of most ice known to exist today. Possible explanations for this may include: (i) observational uncertainties on reported ages and volumes; (ii) longer-term past presence of the Polar Layered Deposits than indicated by reported age estimates; (iii) peak paleo-volumes of mid-latitude glaciers or other extant deposits much larger than their present-day state; (iv) large past volumes of equatorial or mid-latitude ice; (v) missing H 2 O reservoirs that are not well known enough to be included in an inventory at present, such as a large abundance of adsorbed water; and (vi) secular increase of the near-surface ice inventory over the last 1 Gyr. These are not mutually exclusive, but shortcomings exist for each. Determination of a more accurate mass balance history will provide insights into the major events of the Amazonian period.

Icarus↗

Contrasting haemosporidian infections in two ecologically distinct wading birds from breeding colonies in the southeastern United States

Wading birds may serve as ideal hosts for avian hemoparasites, as they are long-lived, undertake extensive movements, form dense breeding colonies, and inhabit water-associated environments that support vectors. Although previous studies have reported parasite species and prevalence in various wading bird species, little is known about their associations with bird behavior and life stage. To address this gap, we examined haemosporidian infections in two ecologically distinct species, white ibis ( Eudocimus albus ) and tricolored heron ( Egretta tricolor ), to explore differences in life stage and movement that may explain prevalence differences. We combined blood screening for hemoparasites with satellite tracking data describing birds’ movement patterns. We screened 95 white ibis (67 juveniles and 28 adults or subadults) and 69 tricolored herons (45 juveniles and 24 adults). We detected a single Haemoproteus plataleae lineage in both species, with higher infection prevalence in white ibis (42.1 %) than in tricolored herons (14.5 %). Among white ibis, adults showed a higher prevalence (67.9 %) than juveniles (31.3 %), whereas in tricolored herons, adults had a prevalence of 8.3 % and juveniles 17.8 %. Non-breeding season movement data showed that white ibis used both freshwater and saline habitats across the southeastern United States, which may also serve as habitats for vectors. In contrast, tricolored herons remained mainly along coastal areas, using saline habitats in the southeastern United States and wintering sites in Central America, which may be less favorable for vectors. Overall, white ibis may serve as major reservoirs and sources of reinfection for H. plataleae , whereas tricolored herons may facilitate parasite dispersal between breeding colonies along the coasts of the southeastern United States and wintering areas in Central America. This study presents the first direct comparison of hemoparasite infections in two ecologically distinct wading birds and highlights movement data as key to explaining infection differences, providing a baseline for future studies.

Alabama↗

Edible baits for systemic flea control, plague mitigation, and wildlife conservation: Evaluation of four active ingredients with three rodent species in western North America

The flea-borne agent of plague, Yersinia pestis , is lethal to endangered black-footed ferrets ( Mustela nigripes , BFFs) and the prairie dogs ( Cynomys spp., PDs) on which BFFs depend for habitat and prey. We developed bait pellets containing insecticides for flea control with PDs. Individual baits contained 0.46, 0.91, 1.21, or 1.52 mg fipronil, 5.40 mg afoxolaner, 50.62 mg fluralaner, or 85.20 mg spinosad. From 2023 to 2025, we tested the baits with black-tailed PDs ( C. ludovicianus , BTPDs), Gunnison's PDs ( C. gunnisoni , GPDs), and Richardson's ground squirrels ( Urocitellus richardsonii , RGSs). We sampled hosts 2810 times and detected 8825 fleas across 2 U.S. States, 1 Canadian Province, 6 sites, 9 PD colonies, and 41 sampling plots. Over ∼12 mo across 5 replicates in South Dakota, USA, bait pellets with 0.91 or 1.52 mg fipronil, applied at a rate of 125 baits/ha, were more effective in reducing the abundance of fleas on BTPDs than 0.46 mg fipronil or the 3 other active ingredients; on 2 South Dakota replicates with data from 24 mo posttreatment, the effects of fipronil pellets on flea abundance had waned after ∼24 mo. Similarly, over ∼12 mo on 2 replicates in Arizona, USA, pellets with 1.52 mg fipronil were more effective in reducing the abundance of fleas on GPDs than pellets with 0.46 mg fipronil; on 1 replicate with available data from ∼2 yr posttreatment, the effects of fipronil pellets had waned after ∼24 mo. Over ∼8-11 mo across 2 replicates in Saskatchewan, Canada, baits with 1.21 mg fipronil/pellet were more effective in suppressing the abundance of fleas on BTPDs and RGSs when applied at 250 pellets/ha than 62 pellets/ha; flea control had waned after ∼20-23 mo. When applied annually at 125-250/ha, baits with 0.84-1.52 mg fipronil (FipBits) provided an effective, efficient, and affordable tool for flea control on PD colonies.

Arizona, Saskatchewan, South Dakota↗

Effects of fipronil bait pellets on two cricetid species: Potential implications for plague mitigation and wildlife conservation

We evaluated the effects of fipronil bait pellets on two cricetids that commonly occupy colonies of black-tailed prairie dogs ( Cynomys ludovicianus ; BTPDs): western deer mice ( Peromyscus sonoriensis ) and northern grasshopper mice ( Onychomys leucogaster ). In one experiment, bait pellets (0.96 mg fipronil/bait) were applied at 75 baits/ha to three 1.44-ha plots on a BTPD colony. Mouse abundance declined by 70% from before to 6-10 d after treatment. In a second experiment, bait pellets (0.46 or 1.52 mg fipronil/bait) were applied at 125 baits/ha to four plots (0.85-1.86 ha) on two BTPD colonies; two non-treated plots were baselines (1.09 and 2.06 ha). From before to 11-15 d after treatment, mouse abundance declined by 51%- 67% on the treated plots vs. a decline of 9% on the non-treated plots. Mouse survival from before to 11-15 d after treatment was 51% lower on the treated plots. In a third experiment, bait pellets (0.84 mg fipronil/bait) were applied at 125 baits/acre on two 1.44-ha plots on a BTPD colony; two 1.44-ha non-treated plots were baselines. Mouse survival from before to 30-44 d after treatment was 45% lower on the treated plots; the abundance of deer mice on the treated plots remained similar from before to 30-44 d after treatment, perhaps due to juvenile recruitment and/or immigration. In a laboratory experiment, 33 deer mice offered one bait pellet (0.86 mg fipronil/bait) consumed 27% of their bait, on average (range = 0-100%). Over 3 d, deer mouse mortality was estimated at 53%; mortality increased with fipronil dose, which averaged 11 mg fipronil/kg body mass (range = 3-46 mg/kg). Brain samples were available from 31 deer mice; all tested positive for fipronil sulfone, the primary mammalian metabolite of fipronil, at 19 to 61,205 ng fipronil sulfone/g. Additional experiments could determine if these findings scale up to larger landscapes.

South Dakota↗

A weight-of-evidence system for integrating monitoring and environmental indicators to guide biological conservation and resource management of native taxa

Many state and federal environmental management agencies are charged with using data-driven evidence to conserve and restore threatened and endangered species that are adversely impacted by humans. Yet, statistical analyses of available environmental indicator data do not always promote unambiguous, ecologically-meaningful, evidence-based decisions for managing native biota. Here we modified our multi-step quantitative adaptive management framework to identify natural and anthropogenic environmental indicators that maintain or threaten native species. In a proof-of-concept example, we quantified how three native stream fish taxa responded to nine environmental indicators based on three criteria (i.e., statistical significance, ecological relevance, and agreement with expectations). The resulting Weight of Evidence (WoE) integration identified five groups of indicators, clarified ambiguous trends, and identified promising future directions that would not have emerged from examining a single analysis or a single taxon alone. Our evaluation and categorization of environmental indicators established the biological foundation on which multi-disciplinary sustainability rests. Our research contribution demonstrates how following a structured integration framework can lead to novel insights that benefit conservation across organisms and systems even when employing relatively simple analytical techniques. Our results address the following often ignored questions: How do we systematically link together different analytical components of the same larger problems? How do we ecologically integrate similar and dissimilar results across analyses? How can we identify generalities by comparing multiple taxa?

Kansas↗

Sedimentological and geochemical characterization of lacustrine deposits of the Babouri-Figuil basin, northern Cameroon: Implications for source rocks distribution and petroleum exploration

The West and Central African Rift System (WCARS) refers to the series of Cretaceous rift basins where commercial hydrocarbon accumulations have been discovered. Some of the WCARS frontier basins are currently being investigated to increase our understanding of these basins in light of new commercial discoveries. The present study was performed in the Babouri-Figuil Basin (BFB), which is genetically related to the WCARS and constitutes an area of interest in terms of petroleum prospecting, where the distribution of petroleum source rocks and potential targets for petroleum exploration across the entire basin is poorly understood. For the current study, an integrated facies analysis along with organic and inorganic geochemical techniques were applied to the basin's Cretaceous deposits with the aim of reconstructing the paleodepositional environment, assessing factors that triggered the input of organic matter, and providing a spatial overview of the organic matter accumulation in the basin based on outcrop samples. An alluvial fan-lacustrine-braided river system is inferred from the facies analysis of the stratigraphic sequence consisting of conglomerate, sandstone, siltstone, limestone, marlstone, and claystone. Bulk analysis of organic matter reveals that black shale and massive claystone are the main prospective petroleum source rocks in the basin. Inorganic geochemical analyses reveal the influence of anoxic conditions, moderate to high primary productivity, and low terrigenous inputs in organic matter enrichment. The formations rich in organic matter are predominantly concentrated in the western and eastern parts of the basin which may represent areas with depressions, characterized by high accommodation space. In terms of the regional context of the WCARS rift basins, typical hydrocarbon exploration in the BFB may target basal-conglomerate, sandstone beds situated directly above or/and interbedded with the Lower Cretaceous source rocks, and the Upper Cretaceous sandstone beds. Basement rocks (granite, granodiorite, and gneisses) and oil shale deposits may represent potential unconventional hydrocarbon exploration. The current integrated study provides an insight that should guide future hydrocarbon exploration campaigns in the basin.

Babouri-Figuil basin↗

Bird guilds exhibit varied responses to floodplain forest restoration in the Colorado River delta, Mexico

Grouping species into guilds can be useful to inform management decisions locally and at broader scales because guilds lack species-specificity. We investigated the response of five breeding bird guilds to riparian habitat restoration in the arid Colorado River delta, based on two decades of bird detections (2002–2021) at 230 bird count stations across 7 routes in actively revegetated (“restored”) sites, and 20 routes in non-actively revegetated (“control”) sites. We used guilds based on habitat associations. We also described changes in vegetation and explored their influence on bird species detections and guild dynamics. Riparian forest bird specialists responded positively to active revegetation, but this positive response was delayed and weaker in a river reach where restoration began later and featured less typical riparian vegetation. Birds associated with wetland habitat showed a positive response to restoration in the wettest reach, which had a baseline of high abundance of wetland birds in control sites and relatively abundant macrophyte cover. Conversely, the abundance of desert scrub bird specialists was highest in the driest and least vegetated restored reach. Generalists only exhibited decreased detections in the wettest restored reach. All this occurred while declines of riparian forest, wetland, desert scrub, and generalist bird species observed over a decade prior to restoration had stabilized in control sites. Detections of birds associated with agricultural fields increased in the study area, irrespective of restoration efforts. Our study demonstrates that the choice of bird guilds as ecological indicators can significantly influence the interpretation of restoration outcomes.

Arizona↗

Characterization and modeling approach for planning restoration strategies in a complex basin affected by acid mine drainage

The management of acid mine drainage (AMD) impacted catchments, such as the Odiel River basin, in southwestern Spain, prioritizes reclamation to meet water resources needs. Assessing water composition across its watercourses is needed to identify major AMD contributors and potentially guide remediation efforts. An equilibrium-based mixing model was developed to simulate AMD pollutant load reductions and estimate the impact of selective restorations on water quality in the Odiel River, particularly at the planned Alcolea Reservoir near its outlet. Sampling under varying flow conditions (average vs. high flow) showed a reduction in acidity transport (from 54.0 to 42.5 ton/day), attributed to greater neutralization effects during high flows. Over 90 % of metal-fluxes originate alone from the Riotinto (73.6 %), Tharsis (14.5 %), and San Telmo (5.00 %) mining districts, among many other mines. While geochemical model estimates fit well with observed data (R 2 = 0.99), some deviations in non-conservative constituents (i.e. pH, Fe and Al) were observed (R 2 = 0.73–0.99), likely due to uncertainties in solubility constants and redox/Fe speciation. After evaluating model reliability, two reduction scenarios (50 % and 100 %) were applied to the three primary sources. Full removal of contamination could subtantially improve impounded reservoir water quality, with pH values of 4.93 and 7.64, and a net acidity between 8.75 and 4.63 mg/L eq. CaCO 3 in both average and high flows, respectively. Such differences may be related to flow regime effects on water quality. However, a 50 % reduction is insufficient to meet drinking or irrigation standards, highlighting the need for full and appropriate reclamation. The model offers a management tool for decision-making in the restoration of the Odiel River basin and could be transferable to similar AMD-affected basins worldwide.

Odiel River basin, southwestern Spain↗

Unexpected appetites: Metabarcoding reveals grass carp (Ctenopharyngodon idella) foraging behaviors in the Great Lakes

Grass carp ( Ctenopharyngodon idella ) are herbivorous fish introduced to North America in the 1960s, and they are now established throughout the Mississippi River basin and Laurentian Great Lakes. Despite increasingly intensified management efforts, limited information exists on their foraging ecology. We used DNA metabarcoding to characterize the gut contents of grass carp captured from 2019 to 2022 to evaluate spatial, temporal, and environmental patterns in diet composition across Lake Erie and Lake Michigan. Contrary to long-standing assumptions that grass carp primarily consume submerged aquatic vegetation (SAV), results from Lake Erie’s western and central basins revealed a diet dominated by terrestrial and terrestrial-wetland plant genera, including maples ( Acer ), willows ( Salix ), cottonwoods ( Populus ), and nightshades ( Solanum ). In contrast, fish harvested from Lake Erie’s eastern basin and Lake Michigan tributaries consumed more SAV, particularly pondweeds like Potamogeton and Stuckenia . Diet composition varied by basin, river, and year, but not by ploidy (diploid vs. triploid). Distance-based redundancy analyses identified environmental variables as drivers of diet variability. These findings reframe previous risk assessments which were based on diets from small artificial environments, suggesting greater dietary plasticity and revealing previously overlooked terrestrial-aquatic food web connections. Results presented here could inform management actions and sampling locations. Understanding grass carp foraging behavior in invaded systems can provide a critical tool for refining removal strategies, preventing further expansion, and improving future ecological risk assessments.

Great Lakes↗

Competition between invasive round goby and native slimy sculpin in a coldwater laboratory setting

Slimy sculpin ( Cottus cognatus ) abundance has substantially declined in many regions of the Laurentian Great Lakes coincident with the arrival and range expansions of invasive round goby ( Neogobius melanostomus ). Previous studies have shown that round goby can outcompete native mottled sculpin ( C. bairdii ) for shelter and negatively affect native spoonhead sculpin ( C. ricei ) growth, but direct behavioral interactions of slimy sculpin and round goby have not been observed. We conducted a laboratory experiment in which a pair of slimy sculpin were placed in an aquarium that contained an artificial spawning shelter and one round goby (treatment, n = 10) or no other fish (control, n = 10) for at least 30 days at temperatures reflective of their potential offshore overlap. We measured slimy sculpin egg deposition, aggressive interactions, artificial shelter occupancy, growth, and survival. Slimy sculpin were less likely to occupy shelters and experienced increased intraspecific competition when a round goby was present. In contrast, round goby rarely chased slimy sculpin, slimy sculpin were more likely to chase round goby, and slimy sculpin growth, egg deposition, and survival were not significantly different between groups. Differences in density between treatments and the relative size of fish could confound our observations. However, the dominant slimy sculpin occupied the shelter at similar rates between experimental groups if the round goby was smaller. Our results indicate that larger round goby overlapping with slimy sculpin could affect slimy sculpin shelter and space use, but the Great Lakes environment may create more stress for slimy sculpin than we observed.

Lake Michigan, Lake Superior↗