USGS Science⌕ Search

SEARCH · USGS Science

Results for “Physiology & Behavior”

Search indexed USGS publications on groundwater, aquifers, geologic maps, mineral resources and earthquakes. Explore source records by subject and place.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 163 records · Page 9Linked to original sources
USGS2008

Endocrine effects of the herbicide linuron on the American Goldfinch ( Carduelis tristis )

Certain contaminants alter normal physiological function, morphology, and behavior of exposed organisms through an endocrine mechanism. We evaluated how the herbicide linuron, an endocrine-active compound, affects physiological parameters and secondary sex characteristics of the American Goldfinch ( Carduelis tristis ). When administered at relatively low doses (control, 1.0, 4.0, and 16.0 μg linuron per gram of body mass per day), linuron delayed prealternate molt progression in a dose-dependent manner. At the high dose level, linuron exposure lowered hematocrit and female plasma thyroxine concentrations and increased body mass. Neither plasma testosterone concentrations nor the color of plumage or integument of birds in the treatment groups were different from those of the control group. Overall, the physiological effects that were measured suggested disruption of thyroid function. These results highlight the importance of continual monitoring of avian populations for potential effects of exposure to pesticides and other chemicals at sublethal concentrations.

The Auk↗
USGS1976

Physiological response of juvenile coho salmon (Oncorhynchus kisutch) and rainbow trout (Salmo gairdneri) to handling and crowding stress in intensive fish culture

Moving 4–5-in. coho salmon ( Oncorhynchus kisutch ) held in soft (20 ppm CaCO 3 ) water from the relatively light loading density of 0.5 lb/ft 3 to 1, 2, or 4 lb/ft 3 (density index, DI = 0.1, 0.2, 0.4, 0.8) caused significant stress as indicated by loss of feeding behavior, but only minimal physiological disturbances, as indicated by lack of hyperglycemia or hypochloremia. However, moving them to 6 or 12 lb/ft 3 (DI = 1.2, 2.4) caused significant physiological stress which required at least a week for recovery. Smolting coho salmon were physiologically stressed by population densities of 1 lb/ft 3 or more and a subclinical corynebacterial kidney infection was activated. Rainbow trout ( Salmo gairdneri ) (4–5 in.) were physiologically stressed when moved and held at 1 lb/ft 3 or more but retained normal feeding behavior. This indicates that handling and crowding stress will be minimized in softwater areas if densities in fish distribution trucks or in ponds or raceways during disease treatments are held to 0.1–0.5 lb/gal.

Journal of the Fisheries Research Board of Canada↗
USGS2013

Comparing mechanisms of host manipulation across host and parasite taxa

Parasites affect host behavior in several ways. They can alter activity, microhabitats or both. For trophically transmitted parasites (the focus of our study), decreased activity might impair the ability of hosts to respond to final-host predators, and increased activity and altered microhabitat choice might increase contact rates between hosts and final-host predators. In an analysis of trophically transmitted parasites, more parasite groups altered activity than altered microhabitat choice. Parasites that infected vertebrates were more likely to impair the host’s reaction to predators, whereas parasites that infected invertebrates were more likely to increase the host’s contact with predators. The site of infection might affect how parasites manipulate their hosts. For instance, parasites in the central nervous system seem particularly suited to manipulating host behavior. Manipulative parasites commonly occupy the body cavity, muscles and central nervous systems of their hosts. Acanthocephalans in the data set differed from other taxa in that they occurred exclusively in the body cavity of invertebrates. In addition, they were more likely to alter microhabitat choice than activity. Parasites in the body cavity (across parasite types) were more likely to be associated with increased host contact with predators. Parasites can manipulate the host through energetic drain, but most parasites use more sophisticated means. For instance, parasites target four physiological systems that shape behavior in both invertebrates and vertebrates: neural, endocrine, neuromodulatory and immunomodulatory. The interconnections between these systems make it difficult to isolate specific mechanisms of host behavioral manipulation.

Journal of Experimental Biology↗
USGS2015

Inferring spatial and temporal behavioral patterns of free-ranging manatees using saltwater sensors of telemetry tags

Diving or respiratory behavior in aquatic mammals can be used as an indicator of physiological activity and consequently, to infer behavioral patterns. Five Antillean manatees, Trichechus manatus manatus , were captured in Chetumal Bay and tagged with GPS tracking devices. The radios were equipped with a micropower saltwater sensor (SWS), which records the times when the tag assembly was submerged. The information was analyzed to establish individual fine-scale behaviors. For each fix, we established the following variables: distance ( D ), sampling interval ( T ), movement rate ( D / T ), number of dives ( N ), and total diving duration (TDD). We used logic criteria and simple scatterplots to distinguish between behavioral categories: ‘Travelling’ ( D / T ≥ 3 km/h), ‘Surface’ (↓TDD, ↓ N ), ‘Bottom feeding’ (↑TDD, ↑ N ) and ‘Bottom resting’ (↑TDD, ↓ N ). Habitat categories were qualitatively assigned: Lagoon, Channels, Caye shore, City shore, Channel edge, and Open areas. The instrumented individuals displayed a daily rhythm of bottom activities, with surfacing activities more frequent during the night and early in the morning. More investigation into those cycles and other individual fine-scale behaviors related to their proximity to concentrations of human activity would be informative

Chetumal Bay↗
USGS2016

Sex differences in contaminant concentrations of fish: a synthesis

Comparison of whole-fish polychlorinated biphenyl (PCB) and total mercury (Hg) concentrations in mature males with those in mature females may provide insights into sex differences in behavior, metabolism, and other physiological processes. In eight species of fish, we observed that males exceeded females in whole-fish PCB concentration by 17 to 43%. Based on results from hypothesis testing, we concluded that these sex differences were most likely primarily driven by a higher rate of energy expenditure, stemming from higher resting metabolic rate (or standard metabolic rate (SMR)) and higher swimming activity, in males compared with females. A higher rate of energy expenditure led to a higher rate of food consumption, which, in turn, resulted in a higher rate of PCB accumulation. For two fish species, the growth dilution effect also made a substantial contribution to the sex difference in PCB concentrations, although the higher energy expenditure rate for males was still the primary driver. Hg concentration data were available for five of the eight species. For four of these five species, the ratio of PCB concentration in males to PCB concentration in females was substantially greater than the ratio of Hg concentration in males to Hg concentration in females. In sea lamprey (Petromyzon marinus), a very primitive fish, the two ratios were nearly identical. The most plausible explanation for this pattern was that certain androgens, such as testosterone and 11-ketotestosterone, enhanced Hg-elimination rate in males. In contrast, long-term elimination of PCBs is negligible for both sexes. According to this explanation, males ingest Hg at a higher rate than females, but also eliminate Hg at a higher rate than females, in fish species other than sea lamprey. Male sea lamprey do not possess either of the above-specified androgens. These apparent sex differences in SMRs, activities, and Hg-elimination rates in teleost fishes may also apply, to some degree, to higher vertebrates including humans. Our synthesis findings will be useful in: (1) developing sex-specific bioenergetics models for fish, (2) developing sex-specific risk assessment models for exposure of humans and wildlife to contaminants, and (3) refining Hg mass balance models for fish and higher vertebrates.

Biology of Sex Differences↗
USGS1999

Tadpoles: the Biology of Anuran Larvae

The recent alarming declines in amphibian populations worldwide and the suitability of amphibians for use in answering research questions in disciplines as diverse as molecular systematics, animal behavior, ecology, and evolutionary biology have focused enormous attention on tadpoles. Yet despite this growing interest, relatively little is known about these fascinating creatures. n this invaluable reference, leading experts on tadpole biology relate what we currently know about tadpoles and what we might learn from them in the future. Tadpoles provides detailed summaries of tadpole morphology, development, behavior, ecology, and environmental physiology; explores the evolutionary consequences of the tadpole stage; synthesizes available information on their biodiversity, and presents a standardized terminology and an exhaustive literature review of tadpole biology.

Book↗
USGS2000

Contaminant effect endpoints in terrestrial vertebrates at and above the individual level

Use of biochemical, physiological, anatomical, reproductive and behavioral characteristics of wild terrestrial vertebrates to assess contaminant exposure and effects has become commonplace over the past 3 decades. At the level of the individual organism, response patterns have been associated with and sometimes causally linked to contaminant exposure. However, such responses at the organismal level are rarely associated with or causally linked to effects at the population level. Although the ultimate goal of ecotoxicology is the protection of populations, communities, and ecosystems, most of the existing science and regulatory legislation focus on the level of the individual. Consequently, much of this overview concentrates on contaminant effects at the organismal level, with some extrapolation to higher-level effects. In this chapter, we review the state of the science for the evaluation of biotic end-points used to assess contaminant exposure and effects at or above the level of the individual. In addition, we describe extant contaminant concentration thresholds, guidelines, or standards (toxicant criteria) in environmental matrices (e.g., water, soil, sediment, foods) that have been developed to protect wild terrestrial vertebrates. Suggestions are provided to develop and embellish the use and value of such endpoints and criteria for extrapolation of effects to higher levels of biological organization. Increasing focus on populations, communities, and ecosystems is needed to develop biologically meaningful regulatory guidelines that will protect natural resources.

Book chapter↗
USGS2020

Growth and behavior of North American microbes on Phragmites australis leaves

Phragmites australis subsp. australis is a cosmopolitan wetland grass that is invasive in many regions of the world, including North America, where it co-occurs with the closely related Phragmites australis subsp. americanus. Because the difference in invasive behavior is unlikely to be related to physiological differences, we hypothesize that interactions with unique members of their microbiomes may significantly affect the behavior of each subspecies. Therefore, we systematically inoculated both plant lineages with a diverse array of 162 fungal and bacterial isolates to determine which could (1) differentiate between Phragmites hosts, (2) infect leaves at various stages of development, or (3) obtain plant-based carbon saprophytically. We found that many of the microbes isolated from Phragmites leaves behave as saprophytes. Only 1% (two taxa) were determined to be strong pathogens, 12% (20 taxa) were weakly pathogenic, and the remaining 87% were nonpathogenic. None of the isolates clearly discriminated between host plant lineages, and the Phragmites cuticle was shown to be a strong nonspecific barrier to infection. These results largely agree with the broad body of literature on leaf-associated phyllosphere microbes in Phragmites.

Microorganisms↗
USGS2017

Book review: Behavioral ecology of the eastern red-backed salamander: 50 years of research

In commemoration of the 100th anniversary of the British Ecological Society, Sutherland et al. (2013) identified 100 questions of fundamental significance in “pure” (i.e., not applied) ecology. A somewhat unexpected outcome of these authors’ exercise was the realization that, after 100 years of comprehensive, intensive scientific research, there remained “profound knowledge gaps” in ecology, such as a clear understanding of “the central mechanisms driving ecosystems…communities…, and even population dynamics.” Animal behavior (along with other attributes such as physiology and genetics) is such a mechanism that can structure ecological interactions, and the study of behavioral ecology provides important insights into many fundamental ecological phenomena. For example, the well-known historical characterization of ecology as the study of the distribution and abundance of organisms (Andrewartha and Birch 1954) invokes numerous questions, such as: what factors influence coexistence among competing species, or between predators and their prey? Ultimately, the answers to these and other questions are best addressed with fine-scale, mechanistic studies of habitat selection, foraging behavior/prey selection, and movement/dispersal behavior. Similarly, at the population level, insight into the spatial distribution of individuals could be gained with studies of territoriality, dominance hierarchies, and even mate choice.

Herpetological Review↗
USGS1976

Feeding ecology of breeding gadwalls on saline wetlands

The feeding ecology of breeding gadwalls ( Anas strepera ) from saline wetlands in North Dakota was examined in relation to sex, pair mates, reproductive status, food availability, and wetland type during the spring and summer of 1971 and 1972. Esophagi of males and females contained 40.4 and 48.2 percent animal food, respectively, between 17 April and 25 August. Animal foods consumed by paired females varied with reproductive condition and were independent of their mates. Invertebrates increased from 47.7 i?? 17.4 percent in the diet during prelaying to 72.0 i?? 18.4 percent during laying and declined to 46.3 i?? 30.0 percent during postlaying. Aquatic insects dominated the diet during egg-laying and were selected disproportionately relative to their availability. Esophageal contents indicated that diversity of plant and animal foods in the diet varied inversely with specific conductance. Major factors influencing food selection of the breeding birds are discussed as interactions among their physiological status, their anatomical and behavioral characteristics, and the abundance and behavior of food organisms as influenced by chemical and physical features of the environment. The data suggested that these interrelated ecological factors act simultaneously to control the phenology of events and determine the foods utilized.

North Dakota↗
USGS2009

Warmwater and coldwater fish in two-story stranding waters

Two-story fisheries occur in lakes or reservoirs characterized by two distinct spatial strata, warmwater and coldwater. These strata develop as the system begins to warm in the spring or summer in response to solar radiation and then separate into an upper warmwater stratum (epilimnion, hereafter referred to as the upper stratum) and a lower coldwater stratum (hypolimnion, hereafter referred to as the lower stratum) separated by the thermocline, a zone of rapidly declining temperatures with depth (i.e., stratification; Figure 10.1). Each stratum or story is dominated by fish species with different physiological constraints, dietary preferences, and behavior. The original use of the term “two-story” described lentic systems in which the lower stratum was less than 21°C during summer and dominated by stocked salmonids, the upper stratum was dominated by sunfishes or perches, and attempts were made to simultaneously manage both strata as sport fisheries (e.g., Jones 1982). However, the terminology is now used more generally to describe any two-stratum system where a coldwater species dominates the lower stratum, often piscivores; a warmwater species dominates the upper stratum, often a planktivore or omnivore; and both contribute to the sport fishery. In general, the upper stratum is characterized by a large, relatively shallow, littoral zone where a diverse assemblage of fishes interact with the bottom, whereas the lower stratum is characterized as deep and pelagic with fewer fish species.

Book chapter↗
USGS2025

Cardiac and behavioral responses to chemical and electrical immobilization in Lake Trout

Objective Immobilizing or anesthetizing fish is important for promoting fish welfare in numerous routine activities that involve handling. Electroanesthesia, an alternative to traditional chemical anesthetics, uses weak electrical current to immobilize fish while current is applied, resulting in near-immediate induction and recovery. The physiological effects of electroanesthesia appear to be minimal or comparable to those of chemical anesthetics, but knowledge gaps exist on cardiac responses during treatment and behavioral responses in a controlled setting. Methods Lake Trout Salvelinus namaycush were immobilized by one of four treatments: control (physical restraint); eugenol (AQUI-S 20E); and continuously applied, low-voltage electroanesthesia with either constant DC or transcutaneous electrical nerve stimulation (TENS). We evaluated the heart rate while fish were immobilized, reflexes at 1 h posttreatment, and survival and movements in a laboratory setting over a 6-d posttreatment period. Results The heart rates of fish immobilized with either electroanesthesia treatment (constant DC: mean ± SE = 12.6 ± 1.1 beats/min; TENS: 13.1 ± 1.2 beats/min) were not significantly different from those of control fish (10.0 ± 1.2 beats/min) while immobilized. However, fish immobilized with eugenol exhibited heart rates that were more than three times higher (32.1 ± 1.2 beats/min) than those of control fish while immobilized. Treatments had no effect on reflex scores at 1 h posttreatment, the 6-d survival rate of fish (100%), or movement behavior during the hours (1–24 h) or days (6 d) after treatment. Conclusions This study adds to a growing body of research finding a lack of negative physiological or behavioral responses resulting from continuously applied electroanesthesia, suggesting that it is at least as safe as many chemical anesthetics in this respect. The significant cardiac response of Lake Trout while immobilized with eugenol warrants further investigation. While the body of research on immobilizing agents in fish grows, species-specific data remain sparse, and users would benefit from pilot testing before wider application.

Transactions of the American Fisheries Society↗
USGS2020

Ringed seal (Pusa hispida) seasonal movements, diving, and haul-out behavior in the Beaufort, Chukchi, and Bering Seas (2011–2017)

Continued Arctic warming and sea-ice loss will have important implications for the conservation of ringed seals, a highly ice-dependent species. A better understanding of their spatial ecology will help characterize emerging ecological trends and inform management decisions. We deployed satellite transmitters on ringed seals in the summers of 2011, 2014, and 2016 near Utqiaġvik (formerly Barrow), Alaska to monitor their movements, diving, and haul-out behavior. We present analyses of tracking and dive data provided by 17 seals that were tracked until at least January of the following year. Seals mostly ranged north of Utqiaġvik in the Beaufort and Chukchi Seas during summer before moving into the southern Chukchi and Bering Seas during winter. In all seasons, ringed seals occupied a diversity of habitats and spatial distributions; from near shore and localized, to far offshore and wide-ranging in drifting sea-ice. Continental shelf waters were occupied for >96% of tracking-days, during which repetitive-diving (suggestive of foraging) primarily to the seafloor was the most frequent activity. From mid-summer to early-fall, 12 seals made ~ one-week forays off-shelf to the deep Arctic Basin, most reaching the retreating pack-ice, where they spent most of their time hauled out. Diel activity patterns suggested greater allocation of foraging efforts to midday hours. Haul-out patterns were complementary, occurring mostly at night until April-May when midday hours were preferred. Ringed seals captured in 2011—concurrent with an unusual mortality event (UME) that affected all ice seal species—differed morphologically and behaviorally from seals captured in other years. Speculations about the physiology of molting and its role in energetics, habitat use, and behavior are discussed; along with possible evidence of purported ringed seal ecotypes.

Alaska↗
USGS2009

Ecological Requirements for Pallid Sturgeon Reproduction and Recruitment in the Lower Missouri River: A Research Synthesis 2005-08

This report provides a synthesis of results obtained between 2005 and 2008 from the Comprehensive Sturgeon Research Program, an interagency collaboration between the U.S. Geological Survey, Nebraska Game and Parks Commission, U.S. Fish and Wildlife Service, and the U.S. Army Corps of Engineers' Missouri River Recovery - Integrated Science Program. The goal of the Comprehensive Sturgeon Research Program is to improve fundamental understanding of reproductive ecology of the pallid sturgeon with the intent that improved understanding will inform river and species management decisions. Specific objectives include: *Determining movement, habitat-use, and reproductive behavior of pallid sturgeon; *Understanding reproductive physiology of pallid sturgeon and relations to environmental conditions; *Determining origin, transport, and fate of drifting pallid sturgeon larvae, and evaluating bottlenecks for recruitment of early life stages; *Quantifying availability and dynamics of aquatic habitats needed by pallid sturgeon for all life stages; and *Managing databases, integrating understanding, and publishing relevant information into the public domain. Management actions to increase reproductive success and survival of pallid sturgeon in the Lower Missouri River have been focused on flow regime, channel morphology, and propagation. Integration of 2005-08 Comprehensive Sturgeon Research Program research provides insight into linkages among flow regime, re-engineered channel morphology, and pallid sturgeon reproduction and survival. The research approach of the Comprehensive Sturgeon Research Program integrates opportunistic field studies, field-based experiments, and controlled laboratory studies. The field study plan is designed to explore the role of flow regime and associated environmental cues using two complementary approaches. An upstream-downstream approach compares sturgeon reproductive behavior between an upstream section of the Lower Missouri River with highly altered flow regime to a downstream section that maintains much of its pre-regulation flow variability. The upstream section also has the potential for an experimental approach to compare reproductive behavior in years with pulsed flow modifications ('spring rises') to years without. The reproductive cycle of the female sturgeon requires several years to progress through gonadal development, oocyte maturation, and spawning. Converging lines of evidence support the hypothesis that maturation and readiness to spawn in female sturgeon is cued many months before spawning. Information on reproductive readiness of shovelnose sturgeon indicates that sturgeon at different locations along the Lower Missouri River between St. Louis and Gavins Point Dam are all responding to the same early cue. Although not a perfect surrogate, the more abundant shovelnose sturgeon is morphologically, physiologically, and genetically similar to pallid sturgeon, and thereby provides a useful comparative model for the rarer species. Day length is the likely candidate to define a temporal spawning window. Within the spawning window, one or more additional, short-term, and specific cues may serve to signal ovulation and release of gametes. Of three potential spawning cues - water temperature, water discharge, and day of year - water temperature is the most likely proximate cue because of the fundamental physiological role temperature plays in sturgeon embryo development and survival, and the sensitivity of many fish hormones to temperature change. It also is possible that neither temperature nor discharge is cueing spawning; instead, reproductive behavior may result from the biological clock advancing an individual fish's readiness to spawn day after day through the spawning period until the right moment, independent of local environmental conditions. Separation of the individual effects of discharge events, water temperature, and other possible factors, such as proximity to male

Scientific Investigations Report↗
USGS2023

Long-term assessment of relationships between changing environmental conditions and the physiology of southern Beaufort Sea polar bears (Ursus maritimus)

Climate change is influencing polar bear ( Ursus maritimus ) habitat, diet, and behavior but the effects of these changes on their physiology is not well understood. Blood-based biomarkers are used to assess the physiologic health of individuals but their usefulness for evaluating population health, especially as it relates to changing environmental conditions, has rarely been explored. We describe links between environmental conditions and physiologic functions of southern Beaufort Sea polar bears using data from blood samples collected from 1984 to 2018, a period marked by extensive environmental change. We evaluated associations between 13 physiologic biomarkers and circumpolar (Arctic oscillation index) and regional (wind patterns and ice-free days) environmental metrics and seasonal and demographic co-variates (age, sex, season, and year) known to affect polar bear ecology. We observed signs of dysregulation of water balance in polar bears following years with a lower annual Arctic oscillation index. In addition, liver enzyme values increased over time, which is suggestive of potential hepatocyte damage as the Arctic has warmed. Biomarkers of immune function increased with regional-scale wind patterns and the number of ice-free days over the Beaufort Sea continental shelf and were lower in years with a lower winter Arctic oscillation index, suggesting an increased allocation of energetic resources for immune processes under these conditions. We propose that the variation in polar bear immune and metabolic function is likely indicative of physiologic plasticity, a response that allows polar bears to remain in homeostasis even as they experience changes in nutrition and habitat in response to changing environments.

Alaska↗
USGS2016

Hibernal habitat selection by Wood Frogs ( Lithobates sylvaticus ) in a northern New England montane landscape

Poikilothermic species, such as amphibians, endure harsh winter conditions via freeze-tolerance or freeze-avoidance strategies. Freeze-tolerance requires a suite of complex, physiological mechanisms (e.g., cryoprotectant synthesis); however, behavioral strategies (e.g., hibernal habitat selection) may be used to regulate hibernaculum temperatures and promote overwintering survival. We investigated the hibernal ecology of the freeze-tolerant Wood Frog ( Lithobates sylvaticus ) in north-central Maine. Our objectives were to characterize the species hibernaculum microclimate (temperature, relative humidity), evaluate hibernal habitat selection, and describe the spatial arrangement of breeding, post-breeding, and hibernal habitats. We monitored 15 frogs during two winters (2011/12: N = 10; 2012/13: N = 5), measured hibernal habitat features at micro (2 m) and macro (10 m) spatial scales, and recorded microclimate hourly in three strata (hibernaculum, leaf litter, ambient air). We compared these data to that of 57 random locations with logistic regression models, Akaike Information Criterion, and Kolmogorov–Smirnov tests. Hibernaculum microclimate was significantly different and less variable than leaf litter, ambient air, and random location microclimate. Model averaging indicated that canopy cover (−), leaf litter depth (+), and number of logs and stumps (+; microhabitat only) were important predictors of Wood Frog hibernal habitat. These habitat features likely act to insulate hibernating frogs from extreme and variable air temperatures. For example, decreased canopy cover facilitates increased snowpack depth and earlier snowpack accumulation and melt. Altered winter temperature and precipitation patterns attributable to climate change may reduce snowpack insulation, facilitate greater temperature variation in the underlying hibernacula, and potentially compromise Wood Frog winter survival.

Maine↗
USGS1995

Infectious and parasitic diseases of the gray wolf and their potential effects on wolf populations in North America

Numerous infections and parasitic diseases have been reported for the gray wolf, including more than 10 viral, bacterial, and mycotic disease and more than 70 species of helminths and ectoparasites. However, few studies have documented the role of diseases in population dynamics. Disease can affect wolf populations directly by causing mortality or indirectly by affecting physiological and homeostatic processes, thriftiness, reproduction, behavior, or social structure. In addition, wolves are hosts to diseases that can affect prey species, thus affecting wolf populations indirectly by reducing prey abundance or increasing vulnerability to predation. Diseases such as canine distemper and infectious canine hepatitis are enzootic in wolf populations, whereas rabies occurs in wolves primarily as a result of transmission from other species such as artic and red foxes. Contact between wolves and domestic pets and livestock may affect the composition of diseases in wolves and their effects on wolf populations. Dogs were suspected of introducing lice and canine parovirus to several wolf populations. THe potential for disease to affect wolf populations and other wild and domestic animals should be considered in wolf management plans, particularly in plans for reintroduction of wolves to area within their former range.

Conference Paper↗