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Biological, environmental, and geomorphological factors influencing reach-specific survival of spring Chinook Salmon smolts upstream of the Columbia–Snake River hydrosystem

Objective Pacific salmon Oncorhynchus spp. are exhibiting catastrophic declines throughout much of the Pacific Northwest, and juvenile mortality contributions are disproportionately high. Many Pacific salmon smolt populations are exhibiting high natal stream mortality; however, detailed natal stream reach-specific survival knowledge is lacking. Our objectives were to estimate natal stream reach-specific survival and identify influential covariates for spring Chinook Salmon O. tshawytscha smolts. An additional objective was to evaluate (i.e., estimate postrestoration cumulative survival) a novel and strategic large-scale river restoration approach (i.e., stream confluence restoration) in two Pacific Northwest spring Chinook Salmon natal streams. Methods Using radiotelemetry techniques during March–June (2011–2017), we estimated natal stream reach-specific survival for spring Chinook Salmon smolts in Catherine Creek and the Grande Ronde River, northeast Oregon. We examined the interrelated influences of temporal, biological, environmental, and geomorphological covariates on the behavior, travel time, and reach-specific survival of spring Chinook Salmon smolts that were emigrating through two hydrologically altered natal streams in the interior Columbia River basin. For each natal stream smolt tag-group, Cormack–Jolly–Seber reach-specific survival estimates that were adjusted for premature tag failure (i.e., bias-corrected) were generated. Subsequently, smolt effects were estimated using reach-specific travel times and survival estimates coupled with individual time-varying covariates from radio-tagged smolts and occupied reaches using an existing predator–prey model (i.e., mean free-path length [ XT ] model). Complementary population-specific principal coordinate analyses (PCoAs) were conducted to facilitate visualization of multicovariate resemblances in ordination space. Last, we employed our best-fitting XT model to model cumulative changes in smolt survival that were associated with a large-scale stream channel restoration scenario (i.e., restoration of the historical Catherine Creek and Grande Ronde River confluence). Results In aggregate, Catherine Creek smolts exhibited high mortality throughout Catherine Creek but near-100% survival upon entering the Grande Ronde River. In contrast, Grande Ronde River smolts generally exhibited low mortality upstream from the Grande Ronde Valley and high mortality throughout the Grande Ronde Valley. Our best-fitting XT model indicated that smolt survival was positively correlated with discharge, body size, and current velocity but negatively correlated with avian predation. Natural-origin smolts from Catherine Creek displayed higher λ-values (i.e., distance between predator–prey encounters) than their significantly larger and faster emigrating hatchery conspecifics, indicating that hatchery smolts may be more susceptible to predation. Our population-specific PCoAs further revealed that novel water (i.e., unique water chemistry) and emigration rate were the strongest predictors of reach occupancy in multivariate ordination space. Under a stream restoration scenario of restoring the historical hydrological template (i.e., restored channel configuration), our best-fitting XT model predicted changes in population-specific cumulative survivals to the lower Grande Ronde Valley (Imbler, Oregon) ranging from −38.4% to 69.0% for Grande Ronde River and Catherine Creek spring Chinook Salmon smolts, respectively. Conclusions Our best-fitting XT model and PCoAs identified the relative importance of biological, environmental, and geomorphological factors to both natural- and hatchery-origin Chinook Salmon smolt survival in two neighboring interior natal streams in the Columbia River basin. Our research indicates that concurrent habitat restoration and fish management strategies, including focused attention on smolt-rearing nursery habitat restoration, piscivorous avian control plans, adaptive management strategies for hatchery smolt releases, and discharge and novel water regime restoration projects, may yield survival benefits to “in-basin” Chinook Salmon smolts. Our research can inform origin-type-specific Chinook Salmon smolt management, habitat restoration, and future research decisions throughout the upper Grande Ronde River subbasin and potentially the Pacific Northwest.

Oregon, Washington

A comparative and experimental evaluation of performance of stocked diploid and triploid brook trout

Despite numerous negative impacts, nonnative trout are still being stocked to provide economically and socially valuable sport fisheries in western mountain lakes. We evaluated relative performance and potential differences in feeding strategy and competitive ability of triploid versus diploid brook trout Salvelinus fontinalis in alpine lakes, as well as behavioral and performance differences of diploid and triploid brook trout in two controlled experimental settings: behavioral experiments in the laboratory and performance evaluations in ponds. Across lakes, catch per unit effort (CPUE) and relative weight (Wr ) were not significantly different between ploidy levels. Mean sizes were also similar between ploidy levels except in two of the larger lakes where diploids attained slightly larger sizes (approximately 20 mm longer). We observed no significant differences between diploids and triploids in diet, diet preference, or trophic structure. Similarly, growth and condition did not differ between ploidy levels in smaller-scale pond experiments, and aggressive behavior did not differ between ploidy levels (fed or unfed fish trials) in the laboratory. Independent of ploidy level, the relative performance of brook trout varied widely among lakes, a pattern that appeared to be a function of lake size or a factor that covaries with lake size such as temperature regime or carrying capacity. In summary, we observed no significant differences in the relative performance of brook trout from either ploidy level across a number of indices, systems, and environmental conditions, nor any indication that one group is more aggressive or a superior competitor than the other. Collectively, these results suggest that triploid brook trout will offer a more risk-averse and promising management opportunity when they are stocked to these lakes and elsewhere to simultaneously meet the needs for the sport fishery and conservation objectives.

North American Journal of Fisheries Management

Interactions between walleyes and smallmouth bass in a Missouri River reservoir with consideration of the influence of temperature and prey

Walleyes Sander vitreus are the most popular fish among South Dakota anglers, but smallmouth bass Micropterus dolomieu were introduced to provide new angling opportunities. Some walleye anglers have reported reductions in the quality of walleye fisheries since the introduction of smallmouth bass and attribute this to the consumption of young walleyes by smallmouth bass and competition for shared prey resources. We quantified the diets of walleyes and smallmouth bass in the lower reaches of Lake Sharpe (a Missouri River reservoir), calculated the diet overlap between the two predators, and determined whether they partitioned shared prey based on size. We also quantified walleye diets in the upper reach of the reservoir, which has a different prey base and allowed us to compare the growth rates of walleyes within Lake Sharpe. Age-0 gizzard shad Dorosoma cepedianum composed a substantial proportion of the diets of both predators, regardless of location, for most of the growing season; the patterns in shad vulnerability appeared to drive the observed patterns in diet overlap. Smallmouth bass appeared to consume a smaller size range of gizzard shad than did walleyes, which consumed a wide range. Smallmouth bass consumed Sander spp. in some months, but in very low quantities. Given that global climate change is expected to alter the population and community dynamics in Great Plains reservoirs, we also used a bioenergetics approach to predict the potential effects of limiting prey availability (specifically, the absence of gizzard shad and rainbow smelt Osmerus mordax ) and increased water temperatures (as projected from global climate change models) on walleye and smallmouth bass growth. The models indicated that the absence of rainbow smelt from the diets of walleyes in upper Lake Sharpe would reduce growth but that the absence of gizzard shad would have a more marked negative effect on both predators at both locations. The models also indicated that higher water temperatures would have an even greater negative influence on walleye growth; however, smallmouth bass growth was predicted to increase with higher temperatures. Fisheries managers should consider strategies to enhance the prey base or mitigate the effects of increased water temperatures that may occur in the future as a result of global climate change. Such proactive actions may alleviate potential future competition between walleyes and smallmouth bass resulting from changes in the fish community.

North American Journal of Fisheries Management

Earning their stripes: The potential of tiger trout and other salmonids as biological controls of forage fishes in a western reservoir

Maintaining a balance between predator and prey populations can be an ongoing challenge for fisheries managers, especially in managing artificial ecosystems such as reservoirs. In a high-elevation Utah reservoir, the unintentional introduction of the Utah Chub Gila atraria and its subsequent population expansion prompted managers to experimentally shift from exclusively stocking Rainbow Trout Oncorhynchus mykiss to also stocking tiger trout (female Brown Trout Salmo trutta × male Brook Trout Salvelinus fontinalis ) and Bonneville Cutthroat Trout O. clarkii utah (hereafter, Cutthroat Trout) as potential biological control agents. We measured a combination of diet, growth, temperature, and abundance and used bioenergetic simulations to quantify predator demand versus prey supply. Utah Chub were the predominant prey type for tiger trout, contributing up to 80% of the diet depending on the season. Utah Chub represented up to 70% of the total diet consumed by Cutthroat Trout. Although Utah Chub dominated the fish biomass in the reservoir, we still estimated abundances of 238,000 tiger trout, 214,000 Cutthroat Trout, and 55,000 Rainbow Trout. Consequently, when expanded to the population level of each predator, tiger trout and Cutthroat Trout consumed large quantities of Utah Chub on an annual basis: tiger trout consumed 508,000 kg (2,660 g/predator) of the standing prey population, and Cutthroat Trout consumed an estimated 322,000 kg (1,820 g/predator). The estimated combined consumption by Cutthroat Trout and tiger trout exceeded the estimate of Utah Chub annual production. As such, our results suggest that the high rates of piscivory exhibited by Cutthroat Trout and tiger trout in artificial lentic ecosystems are likely sufficient to effectively reduce the overall abundance of forage fishes and to prevent forage fishes from dominating fish assemblages. Collectively, this research provides the first documented findings on tiger trout ecology and performance, which will aid managers in designing and implementing the best stocking strategy to optimize sport fish performance, control undesirable forage fish, and enhance and maintain angler satisfaction.

Utah

Comparing conventional tagging methods and acoustic telemetry to inform management of Lake Whitefish in Lake Michigan

Objective Studies of fish movement using conventional tags or acoustic telemetry have different benefits and biases that can influence how conclusions are used in a management context. Our objective was to determine whether these two methods provided similar inferences regarding movements and spawning site fidelity of Lake Whitefish Coregonus clupeaformis in Lake Michigan. Additionally, we assessed movement patterns and used telemetry to assess residency time of Lake Whitefish to provide managers with information on which stocks might be exposed to harvest in different regions. Methods Lake Whitefish were tagged during spawning in (1) North and Moonlight bays, (2) Big Bay de Noc, (3) the Menominee River, and (4) the Fox River. Proportions of fish moving between southern Green Bay, northern Green Bay, and Lake Michigan were compared between tag types. Spawning site fidelity was estimated for each tagging site. Seasonal residency indices were calculated using acoustic telemetry detections. Result Estimates differed between the two methods, but overall trends were similar. Fox River fish rarely left southern Green Bay, and fish tagged in North and Moonlight bays rarely entered Green Bay (<10% of individuals). Big Bay de Noc and Menominee River fish moved into other regions more often (>50% of individuals). The residency indices indicated that Big Bay de Noc fish spent most of their time in Lake Michigan while Menominee River fish spent little time in northern Green Bay despite transitioning to the region. Compared to telemetry, conventional tag recoveries underestimated the proportion of individuals moving among regions. Spawning site fidelity estimates (28–100%) varied among tagging groups and between methods. Conclusion Our results suggest that data from conventional tags can inform management at broad geographic scales. However, acoustic telemetry can provide fine-scale information. Information gained from telemetry can be useful in understanding exposure to fishing mortality, which may be valuable for informing management decisions.

Lake Michigan

Lake sturgeon population trends in the St. Clair–Detroit River System, 2001–2019

Lake Sturgeon Acipenser fulvescens are listed as threatened or endangered in 15 states or provinces within their native range. Accordingly, investments in habitat and population restoration for this species have increased throughout the Great Lakes. To aide evaluation of restoration efficacy, robust population parameters are needed to inform management decisions. The St. Clair – Detroit River System (SCDRS) contains one of the largest self-sustaining Lake Sturgeon populations in the Great Lakes; however recent estimates of population abundance and growth parameters have not been assessed. Our study used baited setline and mark-recapture data collected between 2001 – 2019 to estimate whether the number of Lake Sturgeon captured varied annually and/or with water temperature and whether population abundance and population growth rate varied among three sub-populations located in the SCDRS. Trends in the number of Lake Sturgeon captured on setlines varied among sub-populations and by life stage. Annual trends in the number of Lake Sturgeon captured remained consistent over time in the upper St. Clair River, decreased for adults and increased for subadults in the lower St. Clair River, and increased in the Detroit River. With sub-population abundance of 20,184 (95% CI = 12,533 – 27,816) in the upper St. Clair River/southern Lake Huron, 6,523 (95% CI = 5,720 – 7,327) in the lower St. Clair River, and 6,416 (95% CI = 4,065 – 8,767) in the Detroit River, our study confirms that the SCDRS contains the largest Lake Sturgeon population with unimpeded access to the Great Lakes. The geometric mean population growth rate ( λ ) for all sub-populations indicated stable populations and ranged from 1.00 – 1.16. Our study provides an updated assessment of Lake Sturgeon population parameters that serve as a baseline to evaluate habitat restoration efforts and inform management of the SCDRS recreational Lake Sturgeon fishery.

Michigan, Ontario

Demographic characteristics of an adfluvial bull trout population in Lake Pend Oreille, Idaho

Introductions of nonnative species, habitat loss, and stream fragmentation have caused the Bull Trout Salvelinus confluentus to decline throughout much of its native distribution. Consequently, in June 1998, the Bull Trout was listed under the U.S. Endangered Species Act as threatened. The Bull Trout has existed in Lake Pend Oreille and its surrounding tributaries since the last ice age, and the lake once supported a world-renowned Bull Trout fishery. To quantify the current status of the Bull Trout population in Lake Pend Oreille, Idaho, we compared the mean age, growth, maturity, and abundance with reports in a study conducted one decade earlier. Abundance was estimated by mark–recapture for Bull Trout caught in trap nets and gill nets set in Lake Pend Oreille during ongoing suppression netting of Lake Trout S. namaycush in 2007–2008. Bull Trout sampled in 2006–2008 were used to estimate age structure, survival, growth, and maturity. Estimated Bull Trout abundance was similar to that estimated one decade earlier in Lake Pend Oreille. Bull Trout residing in Lake Pend Oreille between 2006 and 2008 were between ages 4 and 14 years; their growth was fastest between ages 1 and 2 and slowed thereafter. Male and female Bull Trout matured at a similar age, but females grew faster than males, thereby maturing at a larger size. Our findings suggest that management has effectively addressed current threats to increase the likelihood of long-term persistence of the Bull Trout population in Lake Pend Oreille.

Idaho

Patterns of live baitfish use and release among recreational anglers in a regulated landscape

The release of live baitfish by anglers has been identified as a high-risk pathway for the introduction of aquatic invasive species due to the potential for invasive fish, invertebrates, or pathogens to be released simultaneously with the baitfish. Consequently, the release of live baitfish is illegal in many jurisdictions, but little is known about compliance rates or angler motivations for illegal release. To assess the incidence of live baitfish release in Minnesota, USA, a state with significant live baitfish use and substantial recreational fisheries, we administered a mail survey to a random sample of 4,000 anglers who held a 2018-2019 annual fishing license and received 671 completed responses. To mitigate potential recall bias, we also administered 345 intercept surveys at waterbody access sites around the state to ask anglers about their current day’s behaviors. A total of 481 (72%) of the mail survey respondents reported that they used live baitfish and of those, 99 (20%) reported that they release their leftover live baitfish into the water at least some of the time. Of the anglers surveyed at waterbody access sites, 59 (19%) were using live baitfish on the day they were surveyed and of those, 11 (18%) released their leftover baitfish into the water. The reasons anglers provided for releasing their baitfish included convenience and their mistaken understanding that released baitfish benefit the recipient ecosystem. The potential for invasive species introductions through baitfish releases is high given the reported rate of baitfish releases. However, there is also significant opportunity for management interventions aimed at changing perceptions and providing convenient disposal alternatives to illegal release to reduce the risk presented by this pathway.

Minnesota

Response of juvenile Chinook Salmon to an experimental reservoir operation to facilitate downstream migration

Objectives High-head dams and storage reservoirs impact ecological processes, impeding recovery efforts of Pacific salmon Oncorhynchus spp. in the western United States. These projects can delay migration, increase risks of predation and disease, and induce mortality for juvenile Pacific salmon during downstream passage. To mitigate these negative effects, large-scale changes to dam operations have been proposed as interim measures or alternatives to engineered fish passage solutions. A novel deep drawdown operation of the Lookout Point Project (two dams and reservoirs) in Oregon serves as a case study for learning from a large-scale management experiment. Beginning in July 2024, Lookout Point Reservoir was slowly lowered to attain minimal levels from mid-November to mid-December with the intent of expediting downstream passage through the project for juvenile Chinook Salmon Oncorhynchus tshawytscha . We evaluated the impacts of the months-long operation on two life histories of juvenile Chinook Salmon with different rearing environments and growth rates. Methods Acoustic telemetry was used to monitor juvenile Chinook Salmon in the Lookout Point Project. Environmental data, including water temperature and hydrological conditions, were collected. We fitted a time-integrated migration survival model to these data to estimate the effects of management-influenced covariates and fish length on apparent survival and residence time in each reach of the Lookout Point Project. Results Larger fish had higher survival than smaller fish in each reach of the Lookout Point Project. Apparent survival through each dam of the Lookout Point Project was highest and forebay residence times lowest during the latter part of the drawdown (late November). However, early periods of the drawdown (September) were associated with a transition from a thermally stratified reservoir to a warm isothermal reservoir that negatively impacted reservoir survival. Conclusions The deep drawdown of the Lookout Point Project achieved the management intent to improve downstream passage for juvenile Chinook Salmon at the lowest reservoir elevations but also caused unintended changes in the reservoir rearing environment prior to full drawdown. Further research could resolve remaining uncertainties regarding the overall effectiveness of drawdown operations with respect to the population dynamics of different juvenile life history strategies. Understanding these factors would help optimize future management actions aimed at Pacific salmon recovery above the Lookout Point Project.

Oregon

Effects of commercial harvest on shovelnose sturgeon populations in the Upper Mississippi River

Shovelnose sturgeon Scaphirhynchus platorynchus have become an increasingly important commercial species in the upper Mississippi River (UMR) because of the collapse of foreign sturgeon (family Acipenseridae) populations and bans on imported caviar. In response to concerns about the sustainability of the commercial shovelnose sturgeon fishery in the UMR, we undertook this study to describe the demographics of the shovelnose sturgeon population and evaluate the influence of commercial harvest on shovelnose sturgeon populations in the UMR. A total of 1,682 shovelnose sturgeon were collected from eight study pools in 2006 and 2007 (Pools 4, 7, 9, 11, 13, 14, 16, and 18). Shovelnose sturgeon from upstream pools generally had greater lengths, weights, and ages than those from downstream pools. Additionally, mortality estimates were lower in upstream pools (Pools 4, 7, 9, and 11) than in downstream pools (Pools 13, 14, 16, and 18). Linear regression suggested that the slower growth of shovelnose sturgeon is a consequence of commercial harvest in the UMR. Modeling of potential management scenarios suggested that a 685-mm minimum length limit is necessary to prevent growth and recruitment overfishing of shovelnose sturgeon in the UMR.

Upper Mississippi River

Trends in relative weight of walleye stocks in Wyoming reservoirs

The relative weight ( W r ) index of body condition provided insight into the stock dynamics of walleye Stizostedion vitreum in six reservoirs in the North Platte River drainage of Wyoming. The three most upstream reservoirs are managed as both walleye and trout ( Oncorhynchus spp. and Salmo spp.) fisheries; trout are stocked annually. The three downstream reservoirs are managed for coolwater and warmwater fishes, and walleye fry are stocked almost annually in two of the reservoirs. Positive relations between stocking densities of trout and W r of walleyes and between water levels and W r of walleyes were observed. Length-related trends in W r within walleye stocks over time were related to prey availability.

Wyoming

Genetic structure and diversity among brook trout from Isle Royale, Lake Nipigon, and three Minnesota tributaries of Lake Superior

Brook trout Salvelinus fontinalis from Isle Royale, Michigan, three Minnesota tributaries of Lake Superior, and Lake Nipigon in Ontario were analyzed for genetic variation at 12 microsatellite DNA loci. Analysis of molecular variance, genetic distance measures, and cluster analysis were used to examine the diversity, gene flow, and relatedness among the samples. The diversity estimates for the samples from Isle Royale were similar to those for the samples collected from Minnesota tributaries of Lake Superior, and all estimates were lower than those reported in other studies of brook trout from eastern North America. Genetic differences were detected among the brook trout at Isle Royale, Lake Nipigon, and the Minnesota tributaries of Lake Superior. Further, the population in Tobin Harbor at the eastern end of Isle Royale was distinct from the populations from tributaries at the southwestern end of the island. The Minnesota tributary population formed a group that was genetically distinct from those from Isle Royale and Lake Nipigon. The Isle Royale population should be managed to preserve the genetic and phenotypic variation that distinguishes it from the other brook trout populations analyzed to date.

Michigan, Minnesota, Ontario

Biology, status, and management of the yellowstone cutthroat trout

Yellowstone cutthroat trout Oncorhynchus clarkii bouvieri were historically distributed in the Yellowstone River drainage (Montana and Wyoming) and the Snake River drainage (Wyoming, Idaho, Utah, Nevada, and probably Washington). Individual populations evolved distinct life history characteristics in response to the diverse environments in which they were isolated after the last glaciation. Anthropogenic activities have resulted in a substantial decline (42% of the historical range is currently occupied; 28% is occupied by core [genetically unaltered] populations), but the number of extant populations, especially in headwater streams, has precluded listing of this taxon under the Endangered Species Act. Primary threats to persistence of Yellowstone cutthroat trout include (1) invasive species, resulting in hybridization, predation, disease, and interspecific competition; (2) habitat degradation from human activities such as agricultural practices, water diversions, grazing, dam construction, mineral extraction, grazing, timber harvest, and road construction; and (3) climate change, including an escalating risk of drought, wildfire, winter flooding, and rising temperatures. Extirpation of individual populations or assemblages has led to increasing isolation and fragmentation of remaining groups, which in turn raises susceptibility to the demographic influences of disturbance (both human and stochastic) and genetic factors. Primary conservation strategies include (1) preventing risks associated with invasive species by isolating populations of Yellowstone cutthroat trout and (2) connecting occupied habitats (where possible) to preserve metapopulation function and the expression of multiple life histories. Because persistence of isolated populations may be greater in the short term, current management is focused on isolating individual populations and restoring habitats; however, this approach implies that humans will act as dispersal agents if a population is extirpated because of stochastic events.

Idaho, Montana, Utah, Wyoming

Channel unit use by Smallmouth Bass: Do land-use constraints or quantity of habitat matter?

I examined how land use influenced the distribution of Smallmouth Bass Micropterus dolomieu in channel units (discrete morphological features—e.g., pools) of streams in the Midwestern USA. Stream segments ( n = 36), from four clusters of different soil and runoff conditions, were identified that had the highest percent of forest ( n = 12), pasture ( n = 12), and urban land use ( n = 12) within each cluster. Channel units within each stream were delineated and independently sampled once using multiple gears in summer 2006. Data were analyzed using a generalized linear mixed model procedure with a binomial distribution and odds ratio statistics. Land use and channel unit were strong predictors of age-0, age-1, and age->1 Smallmouth Bass presence. Each age-class was more likely to be present in streams within watersheds dominated by forest land use than in those with pasture or urban land uses. The interaction between land use and channel unit was not significant in any of the models, indicating channel unit use by Smallmouth Bass did not depend on watershed land use. Each of the three age-classes was more likely to use pools than other channel units. However, streams with high densities of Smallmouth Bass age >1 had lower proportions of pools suggesting a variety of channel units is important even though habitat needs exist at the channel-unit scale. Management may benefit from future research addressing the significance of channel-unit quality as a possible mechanism for how land use impacts Smallmouth Bass populations. Further, management efforts aimed at improving stream habitat would likely be more beneficial if focused at the stream segment or landscape scale, where a variety of quality habitats might be supported.

MIssouri

Using genetic data to estimate capture rate of Wisconsin and Leech Lake strains of Muskellunge stocked in four Wisconsin Lakes

Many inland fisheries are supported by stocking of hatchery-produced fish, and fisheries managers often face difficult decisions regarding strain selection. Stocking evaluations designed to quantify differences in strain performance provide valuable data for designing stocking programs. Here, we use genetic tools to investigate capture rate of two strains of Muskellunge stocked in Wisconsin lakes. We genotyped a total of 1,011 Muskellunge at 13 microsatellites and used data from five reference populations to assign fish stocked in four Wisconsin lakes to their strain of origin. The strains stocked in these lakes were derived from Wisconsin populations in the Upper Chippewa and Wisconsin River drainages and from Leech Lake, Minnesota. Leech Lake Muskellunge demonstrated much lower capture rates than the Wisconsin strain, but results were variable, with a 10% capture rate of Leech Lake strain fish in Lake Monona and 2% capture rate in Lake Wissota despite similar stocking rates (~25%) in both lakes. We hypothesize that the higher capture rates of Wisconsin strain Muskellunge could be due adaptative advantages of the Wisconsin strain in these waters and suggest that managers continue to stock the nearest native (i.e. Wisconsin) strain to achieve the highest return on investment. Keywords: Muskellunge, propagation, Wisconsin, Leech Lake, stocking evaluation, microsatellite, population genetics ms accepted

Wisconsin

Genetic structure and diversity of the endemic Carolina Madtom and conservation implications

Identification and conservation of genetic diversity within and among freshwater fish populations are important to better manage and conserve imperiled species. The Carolina Madtom Noturus furiosus is a small, nongame catfish that is endemic to the Tar and Neuse River basins of North Carolina. Genetic structure has not been studied in the species, and given recent population declines in both basins, identification of remaining genetic diversity within the species is vital for informing conservation efforts. To assess the status and trends of Carolina Madtom genetic structure, we analyzed genetic markers from 173 individuals to (1) define population genetic structure, (2) assess intra- and interbasin genetic differentiation in the Tar and Neuse River basins, and (3) present management implications to guide conservation efforts. Using 10 microsatellite primers developed for the related Yellowfin Madtom N. flavipinnis , we observed low genetic diversity in Carolina Madtoms. Genotype frequencies within samples were not in Hardy–Weinberg equilibrium, with a deficit of heterozygotes that could be due to family structure, inbreeding, or segregation of null alleles. Mean (±SD) M -ratios for the Tar River (0.414 ± 0.117) and Neuse River (0.117 ± 0.102) basin collections indicated that both populations have experienced recent demographic bottlenecks, with that in the Neuse River basin population being more severe. Effective population size estimates for the respective populations were small, on the order of tens of individuals, driving low genetic diversity within populations. However, the multilocus population differentiation metrics (mean ± SE = 0.135 ± 0.031) and D EST (0.125 ± 0.029) were significantly different from zero ( P < 0.001), indicating significant genetic differentiation between the Tar and Neuse River basin populations. Our findings will inform managers on the status of genetic variation in the Carolina Madtom and will guide conservation toward protective listing and management decisions to maintain the viability of this important endemic species.

North Carolina

Factors affecting post-release survival of coded-wire tagged Lake Trout Salvelinus namaycush in Lake Michigan at four historical spawning locations

Since the 1950s, fishery agencies on Lake Michigan have pursued Lake Trout Salvelinus namaycush rehabilitation through Sea Lamprey Petromyzon marinus control, harvest regulations, and by stocking millions of fish annually. Stocking was prioritized at four historically important spawning locations beginning in 1985, and coded wire tags (CWTs) were used to help evaluate performance. We used data from CWT fish captured in fishery-independent surveys from 1998 – 2014 to evaluate relative post-release survival of Lake Trout, estimated by catch-per-unit-effort and corrected for the number of fish stocked (CPUE), across 173 CWT tag lots of the 1994 – 2003 year classes stocked at these four locations. Boosted regression tree (BRT) models were used to assess the relative influence of four variables on Lake Trout CPUE in two age groups (age 4-5 years and 6-10 years) and paired with analyses of variance to test for statistical significance. Genetic strain (29.1%), stocking location (27.8%), mortality at release (23.1%) and predator density (19.9%) had similar influence on the relative survival of younger fish, whereas relative survival of older fish was heavily influenced by stocking location (79.8%). Survival of both age groups was lowest for fish stocked in the Northern Refuge, where the age structure was truncated due to fishery harvest and Sea Lamprey predation. Survival of stocked fish was higher at the Southern Refuge, Clay Banks, and Julian’s Reef, where mortality from sea lamprey and harvest was lower, and where increases in wild Lake Trout have been observed in recent years. Stocked Lake Michigan remnant genetic strains also appeared to survive better than strains from other lakes at these three locations, but strain effects could not be fully disentangled from effects of stocking location, and continued stocking of multiple genetic strains may provide resiliency toward future selection pressures. Continued progress toward rehabilitation will require reducing fishing and lamprey-induced mortality in northern Lake Michigan to build parental stocks of advanced ages as well as balancing efforts among competing management goals.

Lake Michigan

Assessing factors related to Walleye stocking success in the Midwestern United States

Objective The objective of this study was to evaluate stocking success of Walleye Sander vitreus in lakes and reservoirs across the Midwestern United States to inform stocking practices for state agencies. Demand for Walleye stocking may increase if climate change limits the potential for natural recruitment in lakes. Consequently, the strategic distribution of Walleye stocking may maximize fishing opportunities. Methods We synthesized data from 2226 Walleye fry and fingerling stocking events on 653 lakes in the Midwestern United States and used random forest algorithms and mixed-effects linear models to identify abiotic and biotic factors related to Walleye stocking success. Result Latitude and year explained relatively little variation in stocking success compared to within-lake variation. Relative abundance of Largemouth Bass Micropterus nigricans was an important indicator of Walleye stocking success for fry and fingerlings, with stocking success generally decreasing with increased bass abundance. There was an interaction between lake surface area and growing degree-days, as large lakes (>2500 ha) seemed to be more conducive to Walleye stocking success regardless of growing degree-days. The models that we developed did not accurately predict exact levels of Walleye stocking success but were 92–94% accurate in predicting whether the stocking success of both fry and fingerlings would be at or above the 50th percentile. Conclusion These findings may help to inform the management and stocking allocation of Walleye and suggest that future increases in Largemouth Bass abundance and growing degree-days could limit the effectiveness of stocking in some lakes.

Illinois, Indiana, Iowa, Michigan, Minnesota, Nebr