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Permethrin contamination of sawgrass marshes and potential risk for the imperiled Klot’s skipper butterfly (Euphyes pilatka klotsi)

Nontarget effects from mosquito control operations are possible in habitats adjacent to areas targeted by ultra-low-volume (ULV) sprays of permethrin for adult mosquito control. We assessed the risks of permethrin exposure to butterflies, particularly the imperiled Klot's skipper, when exposed to ground-based ULV sprays. Samples of larval host plant leaves (sawgrass) were collected in June (in mosquito season) and January (outside mosquito season) of 2015 from sawgrass marsh habitats of the National Key Deer Wildlife Refuge (Big Pine Key, FL, USA) and analyzed for permethrin. Permethrin detection was higher in June (detected on 70% of samples) than in January (30%), and concentrations were significantly higher in June (geomean = 2.1 ng/g, median = 2.4) relative to January (0.4 ng/g, median = 0.2). Dietary risk for 4th to 5th-instar larvae was low based on the measured residues. The AGricultural DISPersal model (Ver. 8.26) was used to estimate permethrin residues on sawgrass following ULV sprays (deposited residues) to estimate immediate postspray risk. Estimated deposited residues (33–543 ng/g) were much higher than measured residues, which leads to a higher risk likelihood for butterfly larvae immediately after ULV sprays. The difference between estimated and measured residues, and between the two risk estimations, reflects uncertainty in risk estimates based on the measured residues. Research on modeling deposited pesticide residues following ground-based ULV spray is limited. More research on estimating deposited pesticide residues from truck-mounted ULV sprayers could help reduce uncertainty in the risk predictions for nontarget insects like butterflies.

Florida↗

Use of fish telemetry in rehabilitation planning, management, and monitoring in Areas of Concern in the Laurentian Great Lakes

Freshwater ecosystems provide many ecosystem services; however, they are often degraded as a result of human activity. To address ecosystem degradation in the Laurentian Great Lakes, Canada and the United States of America established the Great Lakes Water Quality Agreement (GLWQA). In 1987, 43 highly polluted and impacted areas were identified under the GLWQA as having one or more of 14 Beneficial Use Impairments (BUIs) to the physical and chemical habitat for fish, wildlife and humans, and were designated as Areas of Concern (AOC). Subnational jurisdictions combined with local stakeholders, with support from federal governments, developed plans to remediate and restore these sites. Biotelemetry (the tracking of animals using electronic tags) provides information on the spatial ecology of fish in the wild relevant to habitat management and stock assessment. Here, seven case studies are presented where biotelemetry data were directly incorporated within the AOC Remedial Action Plan (RAP) process. Specific applications include determining seasonal fish–habitat associations to inform habitat restoration plans, identifying the distribution of pollutant-indicator species to identify exposure risk to contamination sources, informing the development of fish passage facilities to enable fish to access fragmented upstream habitats, and assessing fish use of created or restored habitats. With growing capacity for fish biotelemetry research in the Great Lakes, we discuss the strengths and weaknesses of incorporating biotelemetry into AOC RAP processes to improve the science and practice of restoration and to facilitate the delisting of AOCs.

Great Lakes↗

Adaptive management framework and decision support tool for invasive annual bromes in seven Northern Great Plains National Park Service units

National Park Service (NPS) units in the northern Great Plains (NGP) were established to preserve and interpret the history of the United States, protect and showcase unusual geology and paleontology, and provide a home for vanishing large wildlife. A unifying feature among these national parks, monuments, and historic sites is northern mixed-grass prairie, which not only provides background scenery and habitat but is the foundation of many park missions. As recognition of the prairie’s importance to park fundamental resources and values has grown, so too has the realization that invasive plants threaten these values by reducing native species diversity, altering food webs, and marring the visitor experience. Cheatgrass ( Bromus tectorum ) and Japanese brome ( Bromus japonicus )—collectively referred to as “annual bromes”—are of particular concern because of their documented increase through time, and their association with lower native plant diversity, in NGP parks. A variety of grazing, herbicide-application, and prescribed-fire experiments have shown promising short-term results for controlling annual bromes in research-scale plots in the NGP, but it is unclear whether these management actions will be as effective at the larger spatial and longer temporal scales relevant to park management. When uncertainties about the effectiveness of different management actions cannot be answered with traditional research approaches in time to prevent resource degradation, yet recurrent management decisions must be made, an adaptive management approach may be appropriate. Thus, in 2017, we began to develop the ABAM—Annual Brome Adaptive Management—framework. The aim of this framework is to reduce uncertainties about methods for controlling annual bromes in seven NGP parks through a formal process of learning from the application of on-going management. A uniform framework across seven parks provides greater opportunities for reducing these uncertainties compared to a single park acting alone or to multiple parks using different adaptive management frameworks. This technical report details the development and expected implementation of the ABAM framework. After briefly introducing the issue (Section 1) and describing the context in which the framework was developed (Section 2), the report describes how a structured decision-making process was used to frame the problem, determine concrete objectives, and decide the alternative actions for achieving those objectives that the framework would be designed around (Section 3). Then the report describes the process used to develop the ABAM decision support system (Section 4). At the core of this system is the ABAM decision support tool, a Bayesian decision network built on nearly two decades of vegetation monitoring data from NGP parks, as well as current literature and ABAMspecific experiments. This tool, referred to as the ABAM model by its intended users, is built to work with the existing vegetation monitoring, prescribed fire, and invasive plant management programs that support the seven ABAM parks. In Section 5, the report describes how output from the ABAM model is produced and used in annual vegetation management decision making. It describes the ABAM R package (Baldwin et al. 2021) and an example R script that leads a user through an annual workflow using the model and the package. This workflow updates the model with information from new monitoring events following management actions of prescribed fire, herbicide application, or a combination thereof. With the updated model, data describing the current condition of vegetation in park management units, and current data for environmental factors included in the model (soil texture, slope, weather, and grazing), the user then runs the model to predict future vegetation conditions—and managers’ happiness with the outcome—in response to each of 10 management actions for each management unit in each park. These predictions inform managers’ decisions regarding locations and types of management actions to apply in the upcoming year. The ABAM framework is in its infancy, and the report concludes (Section 6) with a discussion of its longer-term viability. Successful adaptive management requires commitment for the long term, likely decades. Currently, the predictions of the decision support tool are not expected to be highly accurate, but they ideally will improve over time as more management actions are applied and their outcomes are captured by monitoring. We designed the ABAM decision support tool to work with the existing management and monitoring resources in ABAM parks to maximize the sustainability of the model’s use, but the ABAM framework requires more than the model. Because this application of an adaptive management framework supported by a quantitative decision support tool to guide vegetation management is unique within the NPS (to our knowledge), institutional knowledge and mechanisms for long-term implementation of the ABAM framework do not exist within the agency. Additionally, the ABAM model and the data that inform it could be improved in a variety of ways. Thus, this report concludes with a discussion of ways to both sustain and improve upon the work completed so far.

Montana, Nebraska, South Dakota, Wyoming↗

Genetic structure of restored Brook Trout populations in the Southern Appalachian Mountains indicates successful reintroductions

Wildlife reintroduction is an important conservation tool for threatened species, yet identifying appropriate source populations poses a challenge. In particular, the possibility of outbreeding depression is cited as a constraint limiting the range of candidate source populations for translocation. When multiple source lineages are mixed during reintroduction, genetic monitoring is necessary to evaluate whether sources contribute equally to subsequent generations and whether they are interbreeding as expected. Moreover, statistical analysis of genetic data should account for complex life histories that might affect the timescale of admixture and genetic drift. Here, we use samples collected over a 23-year period and a stochastic age-structured model to analyze the genetic mixing process in reintroduced Brook Trout ( Salvelinus fontinalis ) populations in the Southern Appalachians. Each restored population was seeded with two to three source populations. Previous research inferred reproductive isolation between source populations leading to a proposal of splitting the species into multiple taxa. In contrast, we found patterns of ancestry that were consistent with random mating and no advantage for one source lineage over any other. Brook Trout from different source streams are mixing as expected in the restoration sites. This result does not support the hypothesis that Brook Trout in the Southern Appalachian Mountains includes several distinct species. Mixing different sources from the same watershed seems to be an effective way to increase genetic diversity of reintroduced populations while minimizing risk to source populations.

North Carolina, Tennessee↗

Integrated rangeland fire management strategy actionable science plan completion assessment: Invasives topic, 2015–20

Loss and degradation of sagebrush rangelands due to an accelerated invasive annual grass-wildfire cycle and other stressors are significant management, conservation, and economic issues in the western United States. These sagebrush rangelands comprise a unique biome spanning 11 states, support over 350 wildlife species, and provide important ecosystem services that include stabilizing the economies of western communities. Impacts to sagebrush ecosystem processes over large areas due to the annual grass-wildfire cycle necessitated the development of a coordinated, science-based strategy for improving efforts to achieve long-term protection, conservation, and restoration of sagebrush rangelands, which was framed in 2015 under the Integrated Rangeland Fire Management Strategy (IRFMS). Central to this effort was the development of an Actionable Science Plan (Plan) that identified 37 priority science needs (Needs) for informing the actions proposed under the 5 topics (Fire, Invasives, Restoration, Sagebrush and Sage-Grouse, Climate and Weather) that were part of the collective focus of the IRFMS. Notable keys to this effort were identification of the Needs co-produced by managers and researchers, and a focus on resulting science being “actionable.” Substantial investments aimed at fulfilling the Needs identified in the Plan have been made since its release in 2016. While the state of the science has advanced considerably, the extent to which knowledge gaps remain relative to identified Needs is relatively unknown. Moreover, new Needs have likely emerged since the original strategy as results from actionable science reveal new questions and possible (yet untested) solutions. A quantifiable assessment of the progress made on the original science Needs can identify unresolved gaps and new information that can help inform prioritization of future research efforts. This report details a systematic literature review that evaluated how well peer-reviewed journal articles and formal technical reports published between January 1, 2015, and December 31, 2020, addressed six needs (hereinafter “Needs”) identified under the Invasives topic in the Plan. The topic outlined research Needs related to the control of invasive plant species in sagebrush rangelands, with a special emphasis on invasive annual grasses. We established the level of progress towards addressing each Need following a standardized set of criteria, and developed summaries detailing how research objectives nested within Needs identified in the Plan (“Next Steps”) were either addressed well, partially addressed, or remain outstanding (that is, addressed poorly) in the literature through 2020. Our searches resulted in the inclusion of 198 science products that at least partially addressed a Need identified in the Invasives topic. The Needs that were well and partially addressed included: studies of natural and anthropogenic factors influencing the distribution and spread; methods of preventing, eradicating and controlling invasive plant species; development of mapping techniques that provide regularly updated annual grass and fine fuel projections; and assessment of the efficacy of potential cheatgrass biocontrol agents. Needs that were addressed poorly included (1) investigations of livestock grazing as a tool for managing invasive plants and (2) investigations of cheatgrass die-offs and identification and subsequent study of potential biocontrol agents associated with those die-offs. The information provided in this assessment will assist updating the Plan along with other science strategies.

western United States↗

Integrated rangeland fire management strategy actionable science plan completion assessment: Restoration topic, 2015–20

Loss and degradation of sagebrush rangelands due to an accelerated invasive annual grass-wildfire cycle and other stressors are substantial management, conservation, and economic issues in the western United States. These sagebrush rangelands comprise a unique biome spanning 11 states, support over 350 wildlife species, and provide important ecosystem services that include stabilizing the economies of western communities. Impacts to sagebrush ecosystem processes over large areas due to the annual grass-wildfire cycle necessitated the development of a coordinated, science-based strategy for improving efforts to achieve long-term protection, conservation, and restoration of sagebrush rangelands, which was framed in 2015 under the Integrated Rangeland Fire Management Strategy (IRFMS). Central to this effort was the development of an Actionable Science Plan (Plan) that identified 37 priority science needs (Needs) for informing the actions proposed under the 5 topics (Fire, Invasives, Restoration, Sagebrush and Sage-Grouse, Climate and Weather) that were part of the collective focus of the IRFMS. Notable keys to this effort were identification of the Needs co-produced by managers and researchers, and a focus on resulting science being “actionable.” Substantial investments aimed at fulfilling the Needs identified in the Plan have been made since its release in 2016. While the state of the science has advanced considerably, the extent to which knowledge gaps remain relative to identified Needs is relatively unknown. Moreover, new Needs have likely emerged since the original strategy as results from actionable science reveal new questions, and possible (yet untested) solutions. A quantifiable assessment of the progress made on the original science Needs can identify unresolved gaps and new information that can help inform prioritization of future research efforts. This report details a systematic literature review that evaluated how well peer-reviewed journal articles and formal technical reports published between January 1, 2015, and December 31, 2020, addressed 10 needs (hereinafter “Needs”) identified under the Restoration topic in the Plan. The topic outlined research Needs for improving restoration success in degraded sagebrush rangelands. We established the level of progress towards addressing each Need following a standardized set of criteria, and developed summaries detailing how research objectives nested within Needs identified in the Plan (“Next Steps”) were either addressed well, partially addressed or remain outstanding (that is, addressed poorly) in the literature through 2020. Our searches resulted in the inclusion of 371 science products that at least partially addressed a Need identified in the Restoration topic. The Needs that were well and partially addressed included: the development of methods to improve seeding success of native plants after fire; evaluation of short- and long-term plant responses relative to biotic and abiotic factors following restoration treatments; assessments of long-term effects on ecosystem processes following conifer removal treatments and development of decision support tools to aid in conifer management; determination of the costs of restoration treatments relative to the benefits to sage-grouse occupancy, survival, and populations; and assessments of the factors which contribute to soil degradation and development of soil enhancement methods that enhance seed germination and seedling survival while resisting nonnative plants. Needs that were not addressed well included: the development of methods for the rapid recovery of sagebrush and native herbaceous plants following wildfire and determination of whether treatments benefit sage-grouse and mitigate impacts from fires; determination of thresholds for successful restoration across a range of environmental conditions and development of decision support tools to assist with restoration effectiveness monitoring; and development and evaluation of the utility of seed transfer zones. The information provided in this assessment will assist updating the Plan along with other science strategies.

western United States↗

Integrated rangeland fire management strategy actionable science plan completion assessment—Fire topic, 2015–20

Loss and degradation of sagebrush rangelands due to an accelerated invasive annual grass-wildfire cycle and other stressors are significant management, conservation, and economic issues in the western United States. These sagebrush rangelands comprise a unique biome spanning 11 states, support over 350 wildlife species, and provide important ecosystem services that include stabilizing the economies of western communities. Impacts to sagebrush ecosystem processes over large areas due to the annual grass-wildfire cycle necessitated the development of a coordinated, science-based strategy for improving efforts to achieve long-term protection, conservation, and restoration of sagebrush rangelands, which was framed in 2015 under the Integrated Rangeland Fire Management Strategy (IRFMS). Central to this effort was the development of an Actionable Science Plan (Plan) that identified 37 priority science needs (Needs) for informing the actions proposed under the 5 topics (Fire, Invasives, Restoration, Sagebrush and Sage-Grouse, Climate and Weather) that were part of the collective focus of the IRFMS. Notable keys to this effort were identification of the Needs co-produced by managers and researchers, and a focus on resulting science being “actionable.” Substantial investments aimed at fulfilling the Needs identified in the Plan have been made since its release in 2016. While the state of the science has advanced considerably, the extent to which knowledge gaps remain relative to identified Needs is relatively unknown. Moreover, new Needs have likely emerged since the original strategy as results from actionable science reveal new questions and possible (yet untested) solutions. A quantifiable assessment of the progress made on the original science Needs can identify unresolved gaps and new information that can help inform prioritization of future research efforts. This report details a systematic literature review that evaluated how well peer-reviewed journal articles and formal technical reports published between January 1, 2015, and December 31, 2020, addressed eight needs (hereinafter known as “Needs”) identified under the Fire topic in the Plan, defined as any non-structure fire that occurs in vegetation or natural fuels, including wildfires and prescribed fires. The topic outlined research Needs broadly focused on understanding the mechanisms and management of threats posed to the maintenance of large, contiguous sagebrush rangelands by fire. We established the level of progress towards addressing each Need following a standardized set of criteria, and developed summaries detailing how research objectives nested within Needs identified in the Plan (‘Next Steps’) were either addressed well, partially addressed or remain outstanding (in other words., addressed poorly) in the literature through 2020. Our searches resulted in the inclusion of 156 science products that at least partially addressed a Need identified in the Fire topic. The Needs that were well and partially addressed included: studies of relationships between fire and the sagebrush ecosystem (Need 1) investigations of the responses of sage-grouse to burned area characteristics (Need 2); spatial modeling of fire risk (Need 3); studies of the effects of fuels management treatments (Need 4); sagebrush reduction treatments on multiple characteristics of the sagebrush ecosystem (Need 6); and assessments of the role of fire in maintaining healthy sagebrush communities (Need 7). Needs addressed poorly included: assessments of the effects of fuel breaks on sage-grouse (Need 5); and investigations of characteristics associated with the effectiveness of fuel breaks (Need 8). The information provided in this assessment will assist updating the Plan along with other science strategies.

western United States↗

Assessment of a habitat equivalency analysis for freshwater mussels in the upper Mississippi River

The upper Mississippi River (UMR) contains diverse, dense, and reproducing assemblages of native freshwater mussels. In the case of an injury to mussels and their habitats, such as a hazardous material spill, train derailment, or barge grounding, resource managers have few restoration strategies. Resource managers need a means to document, quantify, and mitigate adverse effects on mussels resulting from injury. Habitat equivalency analysis (HEA), developed for use with a wide variety of habitat types, is a restoration scaling technique that compares ecological services lost from injury to ecological services gained through restoration actions. The U.S. Fish and Wildlife Service and Iowa Department of Natural Resources modified the HEA for use with native mussels. The mussel HEA has been applied within the UMR to estimate the quantity of restoration needed to compensate the public for injuries to mussels due to contaminant spills and construction projects. Our objective was to describe the UMR HEA for a general audience and assess if the four biological input variables used in the mussel HEA were reasonable based on literature values. We also evaluated the performance of HEA under a range of input scenarios. Although the input estimates used in HEA were within ranges reported in the peer-reviewed literature or were supported by professional judgment in the absence of peer-reviewed literature, outcomes of the mussel HEA were highly variable and would benefit from additional research to reduce uncertainty in the biological inputs. The application of HEA to mussels provides resource managers with a tool to quantify mussel-related ecological services lost from injury and to guide restoration efforts in the UMR.

Freshwater Mollusk Biology and Conservation↗

Scallopleaf sage (salvia vaseyi: Lamiaceae) discovered in Arizona

During the course of field work in Cabeza Prieta National Wildlife Refuge, southwestern Arizona, in 2003, James Cain and Brian Jansen collected Salvia vaseyi, previously known only from the western edge of the Sonoran Desert in California and Baja California. Our findings indicate this shrub might be more widespread in southwestern Arizona mountains. Salvia vaseyi in Arizona seems to represent a relict population. There are other shrubby Salvia in Arizona, but S. vaseyi is the most xeric-mhabiting species and has the narrowest ecological and geographical range.

Journal of the Botanical Research Institute of Tex↗

Model description and evaluation of the mark-recapture survival model used to parameterize the 2012 status and threats analysis for the Florida manatee ( Trichechus manatus latirostris )

This report provides supporting details and evidence for the rationale, validity and efficacy of a new mark-recapture model, the Barker Robust Design, to estimate regional manatee survival rates used to parameterize several components of the 2012 version of the Manatee Core Biological Model (CBM) and Threats Analysis (TA). The CBM and TA provide scientific analyses on population viability of the Florida manatee subspecies ( Trichechus manatus latirostris ) for U.S. Fish and Wildlife Service’s 5-year reviews of the status of the species as listed under the Endangered Species Act. The model evaluation is presented in a standardized reporting framework, modified from the TRACE (TRAnsparent and Comprehensive model Evaluation) protocol first introduced for environmental threat analyses. We identify this new protocol as TRACE-MANATEE SURVIVAL and this model evaluation specifically as TRACE-MANATEE SURVIVAL, Barker RD version 1. The longer-term objectives of the manatee standard reporting format are to (1) communicate to resource managers consistent evaluation information over sequential modeling efforts; (2) build understanding and expertise on the structure and function of the models; (3) document changes in model structures and applications in response to evolving management objectives, new biological and ecological knowledge, and new statistical advances; and (4) provide greater transparency for management and research review.

Open-File Report↗

Integrated rangeland fire management strategy actionable science plan completion assessment— Climate and weather topic, 2015–20

Loss and degradation of sagebrush ( Artemisia spp.) rangelands due to an accelerated invasive annual grass-wildfire cycle and other stressors are significant management, conservation, and economic issues in the western U.S. These sagebrush rangelands comprise a unique biome spanning 11 states, support over 350 wildlife species, and provide important ecosystem services that include stabilizing the economies of western communities. Impacts to sagebrush ecosystem processes over large areas due to the annual grass-wildfire cycle necessitated the development of a coordinated, science-based strategy for improving efforts to achieve long-term protection, conservation, and restoration of sagebrush rangelands, which was framed in 2015 under the Integrated Rangeland Fire Management Strategy (IRFMS). Central to this effort was the development of an Actionable Science Plan (Plan) that identified 37 priority science needs (hereinafter, “Needs”) for informing the actions proposed under the five topics (Fire, Invasives, Restoration, Sagebrush and Sage-Grouse ( Centrocercus urophasianus ), Climate and Weather) that were part of the collective focus of the IRFMS. Notable keys to this effort were identification of the Needs co-produced by managers and researchers, and a focus on resulting science being “actionable.” Substantial investments aimed at fulfilling the Needs identified in the Plan have been made since its release in 2016. While the state of the science has advanced considerably, the extent to which knowledge gaps remain relative to identified Needs is relatively unknown. Moreover, new Needs have likely emerged since the original strategy as results from actionable science reveal new questions and possible (yet untested) solutions. A quantifiable assessment of the progress made on the original science Needs can identify unresolved gaps and new information that can help inform prioritization of future research efforts. This report details a systematic literature review that evaluated how well peer-reviewed journal articles and formal technical reports published between January 1, 2015, and December 31, 2020, addressed four Needs identified under the Climate and Weather topic in the Plan. The topic outlined research Needs broadly focused on understanding the potential effects of climate change on vegetative resilience to inform restoration of sagebrush rangelands. We established the level of progress towards addressing each Need following a standardized set of criteria, and developed summaries detailing how research objectives nested within Needs identified in the Plan (hereinafter, “Next Steps”) were either addressed well, partially addressed or remain outstanding (that is, addressed poorly) in the literature through 2020. Our searches resulted in the inclusion of 92 science products that at least partially addressed a Need identified in the Climate and Weather topic. The Needs that were well and partially addressed included: studies of the complex set of climatic relationships that influence sagebrush rangeland restoration and seeding success; the identification of seed collection areas across the range of environmental variability inhabited by target restoration species; and develop predictive models to assess targeted restoration species’ responses to mid-century climatic conditions. The Need addressed poorly was the identification of native plant species, genotypes and ecotypes, and seed mixes that may be resilient to a changing climate. The information provided in this assessment will assist updating the Plan, and can inform updates of other relevant science planning documents as needed.

Open-File Report↗

Threats to desert tortoise populations: a critical review of the literature

Decision in resource management are generally based on a combination of sociopolitical, economic, and environmental factors, and may be biased by personal values. These three components often contradict each other resulting in controversy. Controversies can usually be reduced when solid scientific evidence is used to support or refute a decision. However, it is important to recognize that data often do little to alter antagonists' positions when differences in values are the bases if the dispute. But, supporting data can make the decision more defensible, both legally and ethically, especially if the data supporting all opposing viewpoints are included in the decision-making process. Resource management decisions must be made using the best scientific information currently available. However, scientific data vary in two important measures of quality: reliability and validity. The reliability of the data is a measure of the degree to which the observations or conclusion can be repeated. Validity of the data is a measure of the degree to which the observation or conclusion reflects what actually occurs in nature. How the data are collected strongly affects the reliability of validity of ecological conclusions that can be made. Research data potentially relevant to management come from different sources, and the source often provides clues to the reliability and, to a certain extent, validity of data. Understanding the quality of data being used to make management decisions helps to separate the philosophical or value-based aspects of arguments from the objective ones, thus helping to clarify the decisions and judgments that need to be made. The West Mojave Plan is a multispecies, bioregional plan for the management of natural resources within a 9.4 million-acre area of the Mojave Desert in California. The plan addresses the legal requirements for the recovery of the desert tortoise ( Gopherus agazzizii ), a threatened species, but also covers an additional approximately 80 species of plants and animals assigned special status by the Bureau of Land Management, U. S. Fish and Wildlife Service, and California Department of Fish and Game. Within the planning area, 28 separate jurisdictions (counties, cities, towns, military installations, etc.) seek programmatic prescriptions that will facilitate streamlined environmental review, result in expedited authorization for development projects, and protect listed and unlisted species into the foreseeable future to avoid or minimize conflicts between proposed development and species' conservation and recovery. All of the scientific data available concerning the biology and management of these approximately 80 species and their habitats must be evaluated to develop a scientifically credible plan. This document provides an overview and evaluation of the knowledge of the major threats to the persistence and recovery of desert tortoise populations. I was specifically asked to evaluate the scientific veracity of the data and reports available. I summarize the data presently available with particular focus on the West Mojave Desert, evaluate the scientific integrity of the data. and identify major gaps in the available knowledge. I do not attempt to provide in-depth details on each study or threat; for more details I encourage the reader to consult the individual papers or reports city throughout this report (many of which are available at most university libraries and at the West Mojave Plan office in Riverside, California). I also do not attempt to characterize or evaluate the past or present management actions, except where they have direct bearing on evaluation of threats, nor do I attempt, for the most part, to acquire, generate, or evaluate new or existing, but uninterpreted data.

California↗

Quantifying thermal-imager effectiveness for detecting bird nests on farms

We conducted a designed experiment to test whether having a thermal-imaging camera available affected researchers' nest detection rates when searching for bird nests in cropland and grassland habitat in an agricultural landscape of Iowa, USA, in 2016. With known active nests present, naïve observers searched for nests with and without a thermal imager available. We did not find a difference in detection probabilities, although only a large difference would have been detectable with our sample size. Extraneous heat signatures from reflected solar radiation and dense vegetation were key factors limiting the usefulness of thermal imagers for locating nests.

Iowa↗

Estimating density and detection of bobcats in fragmented Midwestern landscapes using spatial capture-recapture data from camera traps

Camera-trapping data analyzed with spatially explicit capture–recapture (SCR) models can provide a rigorous method for estimating density of small populations of elusive carnivore species. We sought to develop and evaluate the efficacy of SCR models for estimating density of a presumed low-density bobcat ( Lynx rufus ) population in fragmented landscapes of west-central Illinois, USA. We analyzed camera-trapping data from 49 camera stations in a 1,458-km 2 area deployed over a 77-day period from 1 February to 18 April 2017. Mean operational time of cameras was 52 days (range = 32–67 days). We captured 23 uniquely identifiable bobcats 113 times and recaptured these same individuals 90 times; 15 of 23 (65.2%) individuals were recaptured at ≥2 camera traps. Total number of bobcat capture events was 139, of which 26 (18.7%) were discarded from analyses because of poor image quality or capture of only a part of an animal in photographs. Of 113 capture events used in analyses, 106 (93.8%) and 7 (6.2%) were classified as positive and tentative identifications, respectively; agreement on tentative identifications of bobcats was high (71.4%) among 3 observers. We photographed bobcats at 36 of 49 (73.5%) camera stations, of which 34 stations were used in analyses. We estimated bobcat density at 1.40 individuals (range = 1.00–2.02)/100 km 2 . Our modeled bobcat density estimates are considerably below previously reported densities (30.5 individuals/100 km 2 ) within the state, and among the lowest yet recorded for the species. Nevertheless, use of remote cameras and SCR models was a viable technique for reliably estimating bobcat density across west-central Illinois. Our research establishes ecological benchmarks for understanding potential effects of colonization, habitat fragmentation, and exploitation on future assessments of bobcat density using standardized methodologies that can be compared directly over time. Further application of SCR models that quantify specific costs of animal movements (i.e., least-cost path models) while accounting for landscape connectivity has great utility and relevance for conservation and management of bobcat populations across fragmented Midwestern landscapes.

Illinois↗

Decision analysis to advance environmental sustainability

Decision analysis provides a robust framework for complex decisions related to environmental sustainability and conservation, including for energy and water, fisheries and wildlife management, agriculture, and climate change response. The complexities of these problems stem from their large scope and scale, which leads to multiple decision makers, stakeholders, rightsholders, and other entities with potentially competing objectives. These problems often are time limited (e.g., urgent action is required to prevent species’ extinction), involve management interventions over long time scales and delayed responses to management (deep uncertainty), and are impeded by limited resources (funding, capacity, etc.). In this Special Issue on “Decision Analysis to Advance Environmental Sustainability,” we present five case studies of applications of decision analysis to complex problems in environmental sustainability and conservation. These case studies incorporate multiple objectives related to ecological and environmental sustainability, economic and social concerns, and logistics of implementation. They showcase a wide range of tools and applications to these problems. We also provide suggestions for new avenues of research and application of decision analysis to problems of environmental sustainability and conservation, including how to incorporate other decision-making tools into decision analysis processes, how to broaden the reach of decision analysis to other sustainability problems, how to incorporate more stakeholders and rightsholders into the decision process, the potential to incorporate new technology into these processes, identifying more creative alternatives, how to secure more funding, ways to move from decision to action, and how to move beyond status quo to make big transitions necessary to achieve sustainability.

Decision Analysis↗

Effects of structural marsh management and salinity on invertebrate prey of waterbirds in marsh ponds during winter on the Gulf Coast Chenier Plain

Aquatic invertebrates are important food resources for wintering waterbirds, and prey selection generally is limited by prey size. Aquatic invertebrate communities are influenced by sediments and hydrologic characteristics of wetlands, which were affected by structural marsh management (levees, water-control structures and impoundments; SMM) and salinity on the Gulf Coast Chenier Plain of North America. Based on previous research, we tested general predictions that SMM reduces biomass of infaunal invertebrates and increases that of epifaunal invertebrates and those that tolerate low levels of dissolved oxygen (O2) and salinity. We also tested the general prediction that invertebrate biomass in freshwater, oligohaline, and mesohaline marshes are similar, except for taxa adapted to specific ranges of salinity. Finally, we investigated relationships among invertebrate biomass and sizes, sediment and hydrologic variables, and marsh types. Accordingly, we measured biomass of common invertebrate by three size classes (63 to 199 ??m, 200 to 999 ??m, and ???1000 ??m), sediment variables (carbon content, C:N ratio, hardness, particle size, and O, penetration), and hydrologic variables (salinity, water depth,temperature, 02, and turbidity) in ponds of impounded freshwater (IF), oligohaline (IO), mesohaline (IM), and unimpounded mesohaline (UM) marshes during winters 1997-1998 to 1999-2000 on Rockefeller State Wildlife Refuge, near Grand Chenier, Louisiana, USA. As predicted, an a priori multivariate analysis of variance (MANOVA) contrast indicated that biomass of an infaunal class of invertebrates (Nematoda, 63 to 199 ??m) was greater in UM marsh ponds than in those of IM marshes, and biomass of an epifaunal class of invertebrates (Ostracoda, 200 to 999 ??m) was greater in IM marsh ponds than in those of UM marshes. The observed reduction in Nematoda due to SMM also was consistent with the prediction that SMM reduces invertebrates that do not tolerate low salinity. Furthermore, as predicted, an a priori MANOVA contrast indicated that biomass of a single invertebrate class adapted to low salinity (Oligochaeta, 200 to 999 ??m) was greater in ponds of IF marshes than in those of IO and IM marshes. A canonical correspondence analysis indicated that variation in salinity and O2 penetration best explained differences among sites that maximized biomass of the common invertebrate classes. Salinity was positively correlated with the silt-clay fraction, O2, and O2 penetration, and negatively correlated with water depth, sediment hardness, carbon, and C:N. Nematoda, Foraminifera, and Copepoda generally were associated with UM marsh ponds and high salinity, whereas other invertebrate classes were distributed among impounded marsh ponds and associated with lower salinity. Our results suggest that SMM and salinity have relatively small effects on invertebrate prey of wintering waterbirds in marsh ponds because they affect biomass of Nematoda and Oligochaeta, and few waterbirds consume these invertebrates. ?? 2003, The Society of Wetland Scientists.

Wetlands↗

Understanding the influence of predation by introduced fishes on juvenile salmonids in the Columbia River Basin: Closing some knowledge gaps. Interim Report of Research 2010

Project overview Predation on juvenile salmonids by fish in the Columbia River Basin (CRB) has impacted salmon survival and is a topic that has received considerable attention over the last three decades. Some of the earliest and most detailed research focused on the food habits, consumption rates, abundance, and distribution of predaceous northern pikeminnow Ptychocheilus oregonensis , smallmouth bass Micropterus dolomieu , walleye Sander vitreus , and channel catfish Ictalurus punctatus in John Day Reservoir (Beamesderfer and Rieman 1991; Poe et al. 1991; Vigg et al. 1991). This group of researchers also estimated the loss of juvenile salmonids to predation by some of these predators (Rieman et al. 1991). Since this pioneering effort, others have evaluated various aspects of predation-related mortality on juvenile salmonids in the CRB, focusing mostly on northern pikeminnow and smallmouth bass (e.g., Tabor et al. 1993; Zimmerman 1999; Naughton et al. 2004). Perhaps the most significant finding coming from this body of research was that the native northern pikeminnow was the dominant predator of juvenile salmonids in the CRB. Indeed, Beamesderfer et al. (1996) estimated that northern pikeminnow consumed about 16 million (8%) of the estimated 200 million juvenile salmonids emigrating annually in the CRB, far surpassing the consumption of smallmouth bass, walleye, and channel catfish combined. Because of this, large-scale management fisheries (i.e., the northern pikeminnow management program, or NPMP; see Rieman and Beamesderfer 1990; Beamesderfer et al. 1996) have been implemented in the CRB since 1990 to achieve a 10%–20% exploitation rate on northern pikeminnow and reduce predation on juvenile salmonids. The NPMP has been a success, resulting in up to 38% potential reductions in predation (Friesen and Ward 1999; Knutsen and Ward 1999; Ward and Zimmerman 1999;). In contrast to the NPMP, Oregon and Washington state fish and wildlife agencies manage and enhance recreational fisheries for smallmouth bass and walleye by implementing size and harvest limit regulations. Recently, many biologists and fish managers have become concerned about the impact of non-native predaceous fishes on juvenile salmonid survival. For example, Poe et al. (1994) warned that smallmouth bass, walleye, and channel catfish were expanding their populations in some areas, that these fish could be significant predators on juvenile salmonids, and that they may compete with northern pikeminnow for common prey items, resulting in higher consumption rates of salmonids by the native predator. Sanderson et al. (2009) reported that the impact of non-indigenous species (including piscivorous fishes) on salmon survival within the CRB can be severe and suggested that managing nonindigenous species may be imperative for salmon recovery. Assessing the current ecological impacts of introduced fishes throughout the CRB will fill information gaps associated with their impact on salmonid survival and contribute to the description of CRB food webs. In response to these recent concerns about the potential predatory impact of non-native piscivores on salmon survival, the Bonneville Power Administration (BPA) and the Columbia Basin Fish and Wildlife Authority (CBFWA) co-hosted a workshop to address predation on juvenile salmonids in the CRB by non-native fish (Halton 2008). The purpose of the workshop was to review, evaluate, and develop strategies to reduce predation by non-native fishes on juvenile salmonids. In the end, discussion at the workshop and at subsequent meetings considered two potential ideas to reduce predation by non-native fish on juvenile salmonids; (1) understanding the role of juvenile American shad Alosa sapidissima in the diet of non-native predators in the fall; and (2) the effects of localized, intense reductions of smallmouth bass in areas of particularly high salmonid predation. In this report, we describe initial efforts to understand the influence of juvenile American shad as a prey item for introduced predators in the middle Columbia River. Our first objective, addressed in Chapter 1, was to evaluate the efficacy of nonlethal methods to describe the physiological condition of smallmouth bass, walleye, and channel catfish from late summer through late fall. Such information will be used to understand the contribution of juvenile American shad to the energy reserves of predaceous fish prior to winter. In Chapter 2, we describe the results of some limited sampling to document the food habits of smallmouth bass, walleye, and channel catfish in three reservoirs of the middle Columbia River during late fall. Collectively, we hope to increase our understanding of the contribution of juvenile American shad to the diets of introduced predators and the contribution of this diet to their energy reserves, growth, and perhaps over-winter survival. Managers should be able to use this information for deciding whether to control the population of American shad in the CRB or for managing introduced predaceous fish in the CRB.

Oregon, Washington↗

Status and trends monitoring of the mainstem Columbia River: sample frame development and review of programs relevant to the development of an integrated approach to monitoring

Implementing an Integrated Status and Trends Monitoring program (ISTM) for the mainstem Columbia River will help identify trends in important natural resources and help us understand the long-term collective effects of management actions. In this report, we present progress towards the completion of a stepwise process that will facilitate the development of an ISTM for the mainstem Columbia River. We discuss planning and regulatory documents that can be used to identify monitoring goals and objectives and present existing monitoring and research activities that should be considered as the development of a Columbia River ISTM proceeds. We also report progress towards the development of sample frames for the Columbia and Snake Rivers and their floodplains. The sample frames were formulated using Digital Elevation Models (DEM’s) of the river channel and upland areas and a Generalized Random-Tessellation Stratified (GRTS) algorithm for an area based resource to generate “master sample(s).” Working with the Pacific Northwest Aquatic Monitoring Partnership (PNAMP) we facilitated the transfer of the sample frames to the PNAMP “Monitoring Sample Designer” tool. We then discuss aspects of response and survey designs as they pertain to the formulation of a mainstem Columbia River ISTM. As efforts to formulate an ISTM for the mainstem Columbia River proceed, practitioners should utilize the extensive literature describing the planning and implementation of fish and wildlife mitigation and recovery efforts in the Columbia River Basin. While we make progress towards establishing an ISTM framework, considerable work needs to be done to formulate an ISTM program for the mainstem Columbia River. Long-term monitoring programs have been established for other large rivers systems; scientists that have experience planning, implementing, and maintaining large river monitoring efforts such as those in the Colorado, Illinois, and Mississippi Rivers should be consulted and involved as efforts proceed.

Idaho;Oregon;Washington↗