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Level II scour analysis for Bridge 27 (BRIDTH00490027) on Town Highway 049, crossing Broad Brook, Bridgewater, Vermont

This report provides the results of a detailed Level II analysis of scour potential at structure BRIDTH00490027 on town highway 49 crossing Broad Brook, Bridgewater, Vermont (figures 1–8). A Level II study is a basic engineering analysis of the site, including a quantitative analysis of stream stability and scour (U.S. Department of Transportation, 1993). A Level I study is included in Appendix E of this report. A Level I study provides a qualitative geomorphic characterization of the study site. Information on the bridge available from VTAOT files was compiled prior to conducting Level I and Level II analyses and can be found in Appendix D. The site is in the Green Mountain physiographic province of central Vermont in the town of Bridgewater. The 13.9-mi 2 drainage area is in a predominantly rural and forested basin. In the vicinity of the study site, the left and right banks are pasture with moderate tree cover on the immediate banks. Upstream of bridge 27, a gravel road runs parallel to the left bank. In the study area, the Broad Brook has an incised channel with a slope of approximately 0.007 ft/ft, an average channel top width of 54 ft and an average channel depth of 4 ft. The predominant channel bed materials are gravel and cobble with a D 50 (median diameter) of 77.9 mm or 0.256 ft. The geomorphic assessment at the time of the Level I and Level II site visit on November 9, 1994, indicated that the reach was stable. The town highway 49 crossing of the Broad Brook is a 32-ft-long, one-lane bridge consisting of one 31-ft steel-beam span (Vermont Agency of Transportation, written commun., August 24, 1994). The bridge is supported by vertical, concrete abutments with wingwalls. The left abutment is noted as settled due to previous undermining. Type-2 (less than 3 ft diameter) stone fill protects the upstream left and right wingwalls, the downstream right wingwall, the right abutment, the upstream right road embankment, and the downstream left and right road embankments. Type-3 (less than 4 ft diameter) stone fill protects the downstream left wingwall, but it’s condition was reported as slumping. The channel is skewed approximately 10 degrees to the opening; the opening-skew-to-roadway is also 10 degrees. Additional details describing conditions at the site are included in the Level II Summary and Appendices D and E. Scour depths and rock rip-rap sizes were computed using the general guidelines described in Hydraulic Engineering Circular 18 (Richardson and others, 1993). Scour depths were calculated assuming an infinite depth of erosive material and a homogeneous particle-size distribution. The scour analysis results are presented in tables 1 and 2 and a graph of the scour depths is presented in figure 8.

Vermont↗

Water-level measurements and chloride concentrations for selected wells in Louisiana, January 1988-October 1997

This report presents water - level measurements and chloride concentrations in water from selected wells completed in aquifers in Louisiana . The data were collected during the period January 1988 - October 1997 . Water - level data are presented for 109 wells , and chloride data are presented for 45 wells . Hydrographs and summaries of water - level trends are presented for wells completed in aquifers throughout the State. Chlorographs and summaries of chloride trends are presented for wells completed in the Mississippi River alluvial and Sparta aquifers; Chicot aquifer system; and Gramercy, Norco, and Gonzales-New Orleans aquifers. Data are presented in graphical and tabular formats.

Water-Resources Bulletin↗

Geologic framework for the national assessment of carbon dioxide storage resources—Southern Rocky Mountain Basins: Chapter M in Geologic framework for the national assessment of carbon dioxide storage resources

The U.S. Geological Survey has completed an assessment of the potential geologic carbon dioxide storage resources in the onshore areas of the United States. To provide geological context and input data sources for the resources numbers, framework documents are being prepared for all areas that were investigated as part of the national assessment. This report, chapter M, is the geologic framework document for the Uinta and Piceance, San Juan, Paradox, Raton, Eastern Great, and Black Mesa Basins, and subbasins therein of Arizona, Colorado, Idaho, Nevada, New Mexico, and Utah. In addition to a summary of the geology and petroleum resources of studied basins, the individual storage assessment units (SAUs) within the basins are described and explanations for their selection are presented. Although appendixes in the national assessment publications include the input values used to calculate the available storage resource, this framework document provides only the context and source of the input values selected by the assessment geologists. Spatial-data files of the boundaries for the SAUs, and the well-penetration density of known well bores that penetrate the SAU seal, are available for download with the release of this report.

Arizona, Colorado, Idaho, Nevada, New Mexico, Utah↗

Channel catfish (Ictalurus punctatus) leukocytes express estrogen receptor isoforms ERα and ERβ2 and are functionally modulated by estrogens

Estrogens are recognized as modulators of immune responses in mammals and teleosts. While it is known that the effects of estrogens are mediated via leukocyte-specific estrogen receptors (ERs) in humans and mice, leucocyte-specific estrogen receptor expression and the effects of estrogens on this cell population is less explored and poorly understood in teleosts. Here in, we verify that channel catfish ( Ictalurus punctaus ) leukocytes express ERα and ERβ2. Transcripts of these isoforms were detected in tissue-associated leukocyte populations by PCR, but ERβ2 was rarely detected in PBLs. Expression of these receptors was temporally regulated in PBLs following polyclonal activation by concanavalin A, lipopolysaccharide or alloantigen based on evaluation by quantitative and end-point PCR. Examination of long-term leukocyte cell lines demonstrated that these receptors are differentially expressed depending on leukocyte lineage and phenotype. Expression of ERs was also temporally dynamic in some leukocyte lineages and may reflect stage of cell maturity. Estrogens affect the responsiveness of channel catfish peripheral blood leukocytes (PBLs) to mitogens in vitro . Similarly, bactericidal activity and phorbol 12-myristate 13-acetate induced respiratory burst was modulated by 17β-estradiol. These actions were blocked by the pure ER antagonist ICI 182780 indicating that response is, in part, mediated via ERα. In summary, estrogen receptors are expressed in channel catfish leukocytes and participate in the regulation of the immune response. This is the first time leukocyte lineage expression has been reported in teleost cell lines.

Fish & Shellfish Immunology↗