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Skeletal morphology of the forefoot in shrews (Mammalia: Soricidae) of the genus Cryptotis, as revealed by digital x-rays

Variation in the forefoot skeleton of small-eared shrews (family Soricidae, genus Cryptotis ) has been previously documented, but the paucity of available skeletons for most taxa makes assessment of the degrees of intraspecific and interspecific variation difficult. We used a digital X-ray system to extract images of the forefoot skeleton from 101 dried skins of eight taxa (seven species, including two subspecies of one species) of these shrews. Lengths and widths of each of the four bones of digit III were measured directly from the digital images, and we used these data to quantify variation within and among taxa. Analysis of the images and measurements showed that interspecific variation exceeds intraspecific variation. In fact, most taxa could be distinguished in multivariate and some bivariate plots. Our quantitative data helped us define a number of specific forefoot characters that we subsequently used to hypothesize evolutionary relationships among the taxa using the exhaustive search option in PAUP, a computer program for phylogenetic analysis. The resulting trees generally concur with previously published evolutionary hypotheses for small-eared shrews. Cryptotis meridensis , a taxon not previously examined in recent phylogenies, is rooted at the base of the branch leading to the C. mexicana group of species. The position of this species suggests that the mostly South American C. thomasi group shares an early ancestor with the C. mexicana group.

Journal of Morphology↗

Winter versus summer habitat selection in a threatened ground squirrel

Hibernation is a strategy many species employ to survive periods of thermal stress or resource shortage (e.g., harsh thermal conditions, food limitations) and habitat requirements of hibernating species may differ between summer (the active season) and winter (during hibernation). Accounting for seasonal differences in habitat affinities will help ensure that management actions are more beneficial and land-use policies are more appropriate. The northern Idaho ground squirrel ( Urocitellus brunneus ) is a federally listed threatened species that is in decline and hibernates for approximately 8 months per year. We collared northern Idaho ground squirrels in Adams County, Idaho from 2013–2017. The majority of northern Idaho ground squirrels we collared selected hibernacula outside of the areas they used during the active season. Furthermore, habitat features of hibernacula locations differed from habitat features of active-season areas. Hibernacula locations had greater canopy closure compared to active-season locations (36.9% and 7.0% canopy closure, respectively) and hibernaculum habitat features (particularly distance to nearest log) influenced overwinter survival. Our results suggest that recovery efforts for northern Idaho ground squirrels should include protection and management for the full range of habitat conditions used throughout summer and winter. More broadly, we emphasize the need to identify and protect habitat during all seasons because habitat requirements can differ substantially during different portions of an animal's annual cycle and effective conservation will require management of year-round habitat needs.

Idaho↗

Selection of nest-site habitat by interior least terns in relation to sandbar construction

Federally endangered interior least terns ( Sternula antillarum ) nest on bare or sparsely vegetated sandbars on midcontinent river systems. Loss of nesting habitat has been implicated as a cause of population declines, and managing these habitats is a major initiative in population recovery. One such initiative involves construction of mid-channel sandbars on the Missouri River, where natural sandbar habitat has declined in quantity and quality since the late 1990s. We evaluated nest-site habitat selection by least terns on constructed and natural sandbars by comparing vegetation, substrate, and debris variables at nest sites ( n = 798) and random points ( n = 1,113) in bare or sparsely vegetated habitats. Our logistic regression models revealed that a broader suite of habitat features was important in nest-site selection on constructed than on natural sandbars. Odds ratios for habitat variables indicated that avoidance of habitat features was the dominant nest-site selection process on both sandbar types, with nesting terns being attracted to nest-site habitat features (gravel and debris) and avoiding vegetation only on constructed sandbars, and avoiding silt and leaf litter on both sandbar types. Despite the seemingly uniform nature of these habitats, our results suggest that a complex suite of habitat features influences nest-site choice by least terns. However, nest-site selection in this social, colonially nesting species may be influenced by other factors, including spatial arrangement of bare sand habitat, proximity to other least terns, and prior habitat occupancy by piping plovers ( Charadrius melodus ). We found that nest-site selection was sensitive to subtle variation in habitat features, suggesting that rigor in maintaining habitat condition will be necessary in managing sandbars for the benefit of least terns. Further, management strategies that reduce habitat features that are avoided by least terns may be the most beneficial to nesting least terns.

Missouri River↗

Occupancy and abundance of wintering birds in a dynamic agricultural landscape

Assessing wildlife management action requires monitoring populations, and abundance often is the parameter monitored. Recent methodological advances have enabled estimation of mean abundance within a habitat using presence–absence or count data obtained via repeated visits to a sample of sites. These methods assume populations are closed and intuitively assume habitats within sites change little during a field season. However, many habitats are highly variable over short periods. We developed a variation of existing occupancy and abundance models that allows for extreme spatio‐temporal differences in habitat, and resulting changes in wildlife abundance, among sites and among visits to a site within a field season. We conducted our study in sugarcane habitat within the Everglades Agricultural Area southeast of Lake Okeechobee in south Florida. We counted wintering birds, primarily passerines, within 245 sites usually 5 times at each site during December 2006–March 2007. We estimated occupancy and mean abundance of birds in 6 vegetation states during the sugarcane harvest and allowed these parameters to vary temporally or spatially within a vegetation state. Occupancy and mean abundance of the common yellowthroat ( Geothlypis trichas ) was affected by structure of sugarcane and uncultivated edge vegetation (occupancy = 1.00 [ = 0.96–1.00] and mean abundance = 7.9 [ = 3.2–19.5] in tall sugarcane with tall edge vegetation versus 0.20 [ = 0.04–0.71] and 0.22 [ = 0.04–1.2], respectively, in short sugarcane with short edge vegetation in one half of the study area). Occupancy and mean abundance of palm warblers ( Dendroica palmarum ) were constant (occupancy = 1.00, = 0.69–1.00; mean abundance = 18, = 1–270). Our model may enable wildlife managers to assess rigorously effects of future edge habitat management on avian distribution and abundance within agricultural landscapes during winter or the breeding season. The model may also help wildlife managers make similar management decisions involving other dynamic habitats such as wetlands, prairies, and even forested areas if forest management or fires occur during the field season.

Florida↗

Adapting standardized trout monitoring to a changing climate for the upper Yellowstone River, Montana, USA

Objective Long‐term standardized monitoring programs are fundamental to assessing how fish populations respond to anthropogenic stressors. Standardized monitoring programs may need to adopt new methods to adapt to rapid environmental changes that are associated with a changing climate. In the upper Yellowstone River, Montana, biologists have used a standardized, mark–recapture monitoring protocol to annually estimate the abundance of trout since 1978 to assess population status and trends. However, within the past two decades, climate change has caused changes in discharge timing that have prevented standardized monitoring from occurring annually. Methods We investigated the feasibility of using two analytical methods, N‐mixture models and mean capture probability, for estimating the abundance of three trout species in the upper Yellowstone River using the historical long‐term data set; these methods allow abundance to be estimated when a mark–recapture estimate cannot be obtained due to hydrologic conditions. Result When compared with abundance estimates from mark–recapture methods, N‐mixture models most often resulted in negatively biased abundance estimates, whereas mean capture probability analyses resulted in positively biased abundance estimates. Additionally, N‐mixture models produced negatively biased estimates when tested against true abundance values from simulated data sets. The bias in the N‐mixture model estimates was caused by poor model fit and variation in capture probability. The bias in the mean capture probability estimates was caused by heterogeneity in capture probability, likely caused by variable environmental conditions, which were not accounted for in the models. Conclusion N‐mixture models and mean capture probability are not viable alternatives for estimating abundance in the upper Yellowstone River. Thus, exploring additional adaptations to sampling methodologies and analytical approaches, including models that require individually marked fish, will be valuable for this system. Climate change will undoubtedly necessitate changes to standardized sampling methods throughout the world; thus, developing alternative sampling and analytical methods will be important for maintaining the utility of long‐term data sets.

Montana↗

Investigation of preparation techniques for δ2H analysis of keratin materials and a proposed analytical protocol

Accurate hydrogen isotopic measurements of keratin materials have been a challenge due to exchangeable hydrogen in the sample matrix and the paucity of appropriate isotopic reference materials for calibration. We found that the most reproducible δ 2 H VSMOW‐SLAP and mole fraction of exchangeable hydrogen, x (H) ex , of keratin materials were measured with equilibration at ambient temperature using two desiccators and two different equilibration waters with two sets of the keratin materials for 6 days. Following equilibration, drying the keratin materials in a vacuum oven for 4 days at 60 °C was most critical. The δ 2 H analysis protocol also includes interspersing isotopic reference waters in silver tubes among samples in the carousel of a thermal conversion elemental analyzer (TC/EA) reduction unit. Using this analytical protocol, δ 2 H VSMOW‐SLAP values of the non‐exchangeable fractions of USGS42 and USGS43 human‐hair isotopic reference materials were determined to be –78.5 ± 2.3 ‰ and –50.3 ± 2.8 ‰, respectively. The measured x (H) ex values of keratin materials analyzed with steam equilibration and N 2 drying were substantially higher than those previously published, and dry N 2 purging was unable to remove absorbed moisture completely, even with overnight purging. The δ 2 H values of keratin materials measured with steam equilibration were about 10 ‰ lower than values determined with equilibration in desiccators at ambient temperatures when on‐line evacuation was used to dry samples. With steam equilibrations the x (H) ex of commercial keratin powder was as high as 28 %. Using human‐hair isotopic reference materials to calibrate other keratin materials, such as hoof or horn, can introduce bias in δ 2 H measurements because the amount of absorbed water and the x (H) ex values may differ from those of unknown samples. Correct δ 2 H VSMOW‐SLAP values of the non‐exchangeable fractions of unknown human‐hair samples can be determined with atmospheric moisture equilibration by normalizing with USGS42 and USGS43 human‐hair reference materials when all materials have the same powder size.

Rapid Communications in Mass Spectrometry↗

Field evaluation of a two-dimensinal hydrodynamic model near boulders for habitat calculation

Two-dimensional hydrodynamic models are now widely used in aquatic habitat studies. To test the sensitivity of calculated habitat outcomes to limitations of such a model and of typical field data, bathymetry, depth and velocity data were collected for three discharges in the vicinity of two large boulders in the South Platte River (Colorado) and used in the River2D model. Simulated depth and velocity were compared with observed values at 204 locations and the differences in habitat numbers produced by observed and simulated conditions were calculated. The bulk of the differences between simulated and observed depth and velocity values were found to lie within the likely error of measurement. However, the effect of flow simulation outliers on potential habitat outcomes must be considered when using 2D models for habitat simulation. Furthermore, the shape of the habitat suitability relation can influence the effects of simulation errors. Habitat relations with steep slopes in the velocity ranges found in similar study areas are expected to be sensitive to the magnitude of error found here. Comparison of habitat values derived from simulated and observed depth and velocity revealed a small tendency to under-predict habitat values.

River Research and Applications↗

Effects of dams in river networks on fish assemblages in non-impoundment sections of rivers in Michigan and Wisconsin, USA

Regional assessment of cumulative impacts of dams on riverine fish assemblages provides resource managers essential information for dam operation, potential dam removal, river health assessment and overall ecosystem management. Such an assessment is challenging because characteristics of fish assemblages are not only affected by dams, but also influenced by natural variation and human-induced modification (in addition to dams) in thermal and flow regimes, physicochemical habitats and biological assemblages. This study evaluated the impacts of dams on river fish assemblages in the non-impoundment sections of rivers in the states of Michigan and Wisconsin using multiple fish assemblage indicators and multiple approaches to distinguish the influences of dams from those of other natural and human-induced factors. We found that environmental factors that influence fish assemblages in addition to dams should be incorporated when evaluating regional effects of dams on fish assemblages. Without considering such co-influential factors, the evaluation is inadequate and potentially misleading. The role of dams alone in determining fish assemblages at a regional spatial scale is relatively small (explained less than 20% of variance) compared with the other environmental factors, such as river size, flow and thermal regimes and land uses jointly. However, our results do demonstrate that downstream and upstream dams can substantially modify fish assemblages in the non-impoundment sections of rivers. After excluding river size and land-use influences, our results clearly demonstrate that dams have significant impacts on fish biotic-integrity and habitat-and-social-preference indicators. The influences of the upstream dams, downstream dams, distance to dams, and dam density differ among the fish indicators, which have different implications for maintaining river biotic integrity, protecting biodiversity and managing fisheries.

Wisconsin and Michigan↗

Response of a tall building far from the epicenter of the 11 March 2011 M 9.0 Great East Japan earthquake and aftershocks

The 11 March 2011 M 9.0 Great East Japan earthquake generated significant long-duration shaking that propagated hundreds of kilometers from the epicenter and affected urban areas throughout much of Honshu. Recorded responses of a tall building at 770 km from the epicenter of the mainshock and other related or unrelated events show how structures sensitive to long-period motions can be affected by distant sources. Even when the largest peak input motions to the building is about 3% g , the strong-shaking duration was about 140 s. The 300- to 1000-s prolonged responses of the building are primarily due to a combination of site resonance (e.g. structural fundamental frequency ~0.15 Hz and site frequency ~0.13–0.17 Hz) and low damping (~1–2%) of the structure. Response modification technologies can improve the response of the building during future earthquakes. The need-to-consider risks to such built environments from distant sources are emphasized.

Honshu↗

River otter distribution in Nebraska

The river otter ( Lontra canadensis ) was extirpated from Nebraska, USA, in the early 1900s and reintroduced starting in 1986. Information is needed regarding the distribution of river otters in Nebraska before decisions can be made regarding its conservation status. Understanding distribution of a species is critically important for effective management. We investigated river otter distribution in Nebraska with occupancy modeling and maximum entropy (Maxent) modeling using 190 otter sign observations on Nebraska's navigable rivers and 380 historical otter records from November 1977 to April 2014. Both methods identified the Platte River, Elkhorn River, central and eastern Niobrara River, and southern Loup River system as core areas within the distribution of otters in Nebraska. The Maxent model provided more liberal estimates of site occupancy and identified some smaller rivers as being within the distribution of otters in Nebraska, which were not identified using occupancy modeling. We recommend that multiple data sets and analysis methods be used to estimate species distribution because this allows for the broadest geographical coverage and decreases the likelihood of overlooking areas with fewer animal records. If further reintroduction efforts or translocation efforts are to take place in the future, we recommend focusing on areas with high modeled occupancy but few historical and survey records

Nebraska↗

Behavioral effects of environmental enrichment on harbor seals (Phoca vitulina concolor) and gray seals (Halichoerus grypus)

Zoos and aquariums have been incorporating environmental enrichment into their animal care programs for the past 30 years to increase mental stimulation and promote natural behaviors. However, most attempts to document the effects of enrichment on animal behavior have focused on terrestrial mammals. Staff at the National Aquarium in Baltimore conducted an investigation of the behavioral effects of enrichment on the seven harbor seals and two gray seals housed in the aquarium's outdoor seal exhibit. We expected that enrichment would change the amount of time the animals spent engaged in specific behaviors. The behaviors recorded were: resting in water, resting hauled out, maintenance, breeding display, breeding behavior, aggression, pattern swimming, random swimming, exploration, and out of sight. Activity levels (random swimming and exploration) were expected to increase, while stereotypic behaviors (pattern swimming) were expected to decrease. The frequency and duration of behaviors were documented for 90 hr in both the control phase (without enrichment) and the experimental phase (with enrichment). Statistically significant differences ( P <0.05) in the time spent in pattern swimming, random swimming, exploration, and out of sight were observed between the two phases. With enrichment, pattern swimming and out of sight decreased, while random swimming and exploration behavior increased. These findings demonstrate that enrichment can promote behaviors (random swimming and exploration) that are likely to be normal for phocids in the wild, and that may contribute to the behavioral complexity of these seals in captivity.

Zoo Biology↗

Antibody-producting cells correlated with body weight in juvenile Chinook salmon Oncorhynchus tshawytscha acclimated to optimal and elevated temperatures

The immune response of juvenile chinook salmon ( Oncorhynchus tshawytscha ) ranging in weight from approximately 10 to 55 g was compared when the fish were acclimated to either 13 or 21° C. A haemolytic plaque assay was conducted to determine differences in the number of antibody-producing cells (APC) among fish of a similar age but different body weights. Regression analyses revealed significant increases in the number of APC with increasing body weight when fish were acclimated to either water temperature. These results emphasise the importance of standardising fish weight in immunological studies of salmonids before exploring the possible effects of acclimation temperatures.

Fish & Shellfish Immunology↗

Evidence for growth hormone/insulin-like growth factor I axis regulation of seawater acclimation in the euryhaline teleost Fundulus heteroclitus

The ability of ovine growth hormone (oGH), recombinant bovine insulin-like growth factor I (rbIGF-I), recombinant human insulin-like growth factor II (rhIGF-II), and bovine insulin to increase hypoosmoregulatory capacity in the euryhaline teleost Fundulus heteroclitus was examined. Fish acclimated to brackish water (BW, 10 ppt salinity, 320 mOsm/kg H 2 O) were injected with a single dose of hormone and transferred to seawater (SW, 35 ppt salinity, 1120 mOsm/kg H 2 O) 2 days later. Fish were sampled 24 h after transfer and plasma osmolality, plasma glucose, and gill Na + ,K + -ATPase activity were examined. Transfer from BW to SW increased plasma osmolality and gill Na + ,K + -ATPase activity. Transfer from BW to BW had no effect on these parameters. rbIGF-I (0.05, 0.1, and 0.2 μg/g) improved the ability to maintain plasma osmolality and to increase gill Na + , K + -ATPase activity in a dose-dependent manner. oGH (0.5, 1, and 2 μg/g) also increased hypoosmoregulatory ability but only the higher doses (2 μg/g) significantly increased gill Na + ,K + -ATPase activity. oGH (1 μg/g) and rbIGF-I (0.1 μg/g) had a significantly greater effect on plasma osmolality and gill Na + ,K + -ATPase activity than either hormone alone. rhIGF-II (0.05, 0.1, and 0.2 μg/g) and bovine insulin (0.01 and 0.05 μg/g) were without effect. The results suggest a role of GH and insulin-like growth factor I (IGF-I) in seawater acclimation of F. heteroclitus. Based on these findings and previous studies, it is concluded that the capacity of the GH/IGF-I axis to increase hypoosmoregulatory ability may be a common feature of euryhalinity in teleosts.

Connecticut↗

Distribution and habitat of Nitellopsis obtuse (Characeae) in the Laurentian Great Lakes

Nitellopsis obtusa , a macroalga (Characeae) native to Europe and Asia, was found in U.S. waters of the St. Clair-Detroit River system in 1983, thus extending the range of this taxon into the Laurentian Great Lakes about 850 km from the St. Lawrence River where it was first discovered in North America in 1978. Its occurrence only in water frequented by commercial shipping vessels suggests that it is distributed via this mechanism. In the St. Clair-Detroit River system, N. obtusa was collected with a Ponar grab at four locations, and with a grapnel at one additional location. It was the ninth most frequently found macrophyte and it was most abundant at Belle Isle in the Detroit River, where the mean dry-weight biomass in Ponar samples was 0 g m -2 in June, 37 g m -2 in August, and 32 g m −2 in September. Maximum biomass of this taxon in one Ponar grab at this location was 289 g m -2 in September. The alga occurred primarily in water of relatively low current velocity (11.3 cm s −1 ) and in association with Vallisneria americana, Myriophyllum spicatum, Potamogeton richardsonii, Najas flexilis , and Elodea canadensis .

Hydrobiologia↗

Degradation of the disease-associated prion protein by a serine protease from lichens

The disease-associated prion protein (PrP(TSE)), the probable etiological agent of the transmissible spongiform encephalopathies (TSEs), is resistant to degradation and can persist in the environment. Lichens, mutualistic symbioses containing fungi, algae, bacteria and occasionally cyanobacteria, are ubiquitous in the environment and have evolved unique biological activities allowing their survival in challenging ecological niches. We investigated PrP(TSE) inactivation by lichens and found acetone extracts of three lichen species (Parmelia sulcata, Cladonia rangiferina and Lobaria pulmonaria) have the ability to degrade prion protein (PrP) from TSE-infected hamsters, mice and deer. Immunoblots measuring PrP levels and protein misfolding cyclic amplification indicated at least two logs of reductions in PrP(TSE). Degradative activity was not found in closely related lichen species or in algae or a cyanobacterium that inhabit lichens. Degradation was blocked by Pefabloc SC, a serine protease inhibitor, but not inhibitors of other proteases or enzymes. Additionally, we found that PrP levels in PrP(TSE)-enriched preps or infected brain homogenates are also reduced following exposure to freshly-collected P. sulcata or an aqueous extract of the lichen. Our findings indicate that these lichen extracts efficiently degrade PrP(TSE) and suggest that some lichens could have potential to inactivate TSE infectivity on the landscape or be a source for agents to degrade prions. Further work to clone and characterize the protease, assess its effect on TSE infectivity and determine which organism or organisms present in lichens produce or influence the protease activity is warranted.

PLoS ONE↗

Organochlorine residues in bat guano from nine Mexican caves, 1991

Samples of bat guano, primarily from Mexican free-tailed bats ( Tadarida brasiliensis ), were collected at nine bat roosts in caves in northern and eastern Mexico and analysed for organochlorine residues. DDE, the most abundant residue found in each cave, was highest (0.99 p.p.m. dry weight) at Ojuela Cave, Durango. Other studies of DDE in bat guano indicate that this concentration is too low to reflect harmful concentrations in the bats themselves. The DDE at Ojuela may represent either lingering residues from use of DDT years ago in the Ojuela area of perhaps depuration loss from migrant bats with summer maternity roost(s) in a DDE-contaminated area such as Carlsbad Cavern, New Mexico. Presence of o,p′ -DDT at Tio Bartolo Cave, Nuevo Leon, indicates recent use of DDT, but the concentration of this contaminant was low. Possible impacts on bat colonies of the organophosphorus and carbamate insecticides now in extensive use are unknown.

Ecotoxicology↗

Environmental contaminants in eggs of California least terns (Sterna antillarum browni)

A severe decline in the coastal breeding population of California least terns (Sterna antillarum browni) in California and Baja California (U.S. Fish Wildl. Serv. 1980) prompted both State and Federal governments to designate it an endangered species in 1970 (Massey 1974). Significant losses of nesting and feeding habitat have contributed greatly to the decline of this subspecies (Massey 1974; Atwood and Minsky 1983). However, environmental contaminants, such as organochlorine compounds and metals, may also have contributed to the decline. California least terns are primarily piscivorous during the nesting period (Massey 1974), feeding predominantly on jack-smelt (Atherinops californiensis), topsmelt (A. affinis), and northern anchovy (Engraulis mordax) (Atwood and Minsky 1983). Topsmelt had the highest levels of DDE (p,p'-DDE) (up to 3 #g/g wet wt) of fish collected from San Diego Bay by Ohlendorf et al. (1985). Eggs of Caspian terns ~. caspia) from that study contained up to 56/~g/g DDE, and DDE was associated with a reduction in eggshell thickness as determined by the thickness index. In addition to shell deficiencies, organochlorines can also cause reduced egg production, aberrant incubation behavior, delayed ovulation, embryotoxicosis, and mortality of chicks and adults (Blus 1982). Mercury (Hg) and selenium (Se) have caused decreased hatchability, altered nesting behavior, and embryotoxicosis in birds in field and laboratory studies (Cormors et al. 1975; Ohlendorf et al. 1986; Heinz et al. 1987). Our objective was to evaluate the role of contaminants in the decline of California least terns.

California↗

Reef growth and volcanism on the submarine southwest rift zone of Mauna Loa, Hawaii

A marine sampling program, utilizing the PISCES-5 submersible operated by the Hawaii Undersea Research Laboratory (NOAA), has confirmed the presence of a major submerged coral reef offshore from Ka Lae (South Point), Hawaii. The top of the reef is now 150–160 m below sea level. Radiocarbon and Useries dating indicates that it drowned about 13.9 ka by the combined effects of island subsidence (2.5 mm/year) and the rapid rise of sea level at the end of the last glaciation so that the relative submergence rate of more than 10 mm/year exceeded the upward growth rate of the reef. The submerged reef caps the offshore part of the southwest rift-zone ridge of Mauna Loa, which has apparently undergone little volcanic activity offshore since 170 ka, and possibly since 270 ka. This fact suggests that rift zone activity is becoming increasingly restricted toward the upper part of the volcano, a condition possibly heralding the end of the shield-building stage.

Hawaii↗