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At least 145 records · Page 8Linked to original sources
USGS2007

Comparison of local- to regional-scale estimates of ground-water recharge in Minnesota, USA

Regional ground-water recharge estimates for Minnesota were compared to estimates made on the basis of four local- and basin-scale methods. Three local-scale methods (unsaturated-zone water balance, water-table fluctuations (WTF) using three approaches, and age dating of ground water) yielded point estimates of recharge that represent spatial scales from about 1 to about 1000 m 2 . A fourth method (RORA, a basin-scale analysis of streamflow records using a recession-curve-displacement technique) yielded recharge estimates at a scale of 10–1000s of km 2 . The RORA basin-scale recharge estimates were regionalized to estimate recharge for the entire State of Minnesota on the basis of a regional regression recharge (RRR) model that also incorporated soil and climate data. Recharge rates estimated by the RRR model compared favorably to the local and basin-scale recharge estimates. RRR estimates at study locations were about 41% less on average than the unsaturated-zone water-balance estimates, ranged from 44% greater to 12% less than estimates that were based on the three WTF approaches, were about 4% less than the age dating of ground-water estimates, and were about 5% greater than the RORA estimates. Of the methods used in this study, the WTF method is the simplest and easiest to apply. Recharge estimates made on the basis of the UZWB method were inconsistent with the results from the other methods. Recharge estimates using the RRR model could be a good source of input for regional ground-water flow models; RRR model results currently are being applied for this purpose in USGS studies elsewhere.

Minnesota↗
USGS2007

Recent climate trends and implications for water resources in the Catskill Mountain region, New York, USA

Climate scientists have concluded that the earth’s surface air temperature warmed by 0.6 °C during the 20th century, and that warming induced by increasing concentrations of greenhouse gases is likely to continue in the 21st century, accompanied by changes in the hydrologic cycle. Climate change has important implications in the Catskill region of southeastern New York State, because the region is a source of water supply for New York City. We used the non-parametric Mann–Kendall test to evaluate annual, monthly, and multi-month trends in air temperature, precipitation amount, stream runoff, and potential evapotranspiration (PET) in the region during 1952–2005 based on data from 9 temperature sites, 12 precipitation sites, and 8 stream gages. A general pattern of warming temperatures and increased precipitation, runoff, and PET is evident in the region. Regional annual mean air temperature increased significantly by 0.6 °C per 50 years during the period; the greatest increases and largest number of significant upward trends were in daily minimum air temperature. Daily maximum air temperature showed the greatest increase during February through April, whereas minimum air temperature showed the greatest increase during May through September. Regional mean precipitation increased significantly by 136 mm per 50 years, nearly double that of the regional mean increase in runoff, which was not significant. Regional mean PET increased significantly by 19 mm per 50 years, about one-seventh that of the increase in precipitation amount, and broadly consistent with increased runoff during 1952–2005, despite the lack of significance in the mean regional runoff trend. Peak snowmelt as approximated by the winter–spring center of volume of stream runoff generally shifted from early April at the beginning of the record to late March at the end of the record, consistent with a decreasing trend in April runoff and an increasing trend in maximum March air temperature. This change indicates an increased supply of water to reservoirs earlier in the year. Additionally, the supply of water to reservoirs at the beginning of winter is greater as indicated by the timing of the greatest increases in precipitation and runoff—both occurred during summer and fall. The future balance between changes in air temperature and changes in the timing and amount of precipitation in the region will have important implications for the available water supply in the region.

New York↗
USGS2007

Estimation of evapotranspiration by reed canarygrass using field observations and model simulations

Reed canarygrass (Phalaris arundinacea) commonly invades meadow wetlands, effectively dominating water use and outcompeting native plants. Objectives of this study were to (i) estimate daily, seasonal and annual water use by reed canarygrass using shallow water table fluctuations; and (ii) calibrate the ALMANAC (Agricultural Land Management Alternative with Numerical Assessment Criteria) model to accurately simulate water uptake by this grass. Using a groundwater well, the water table under an area in Iowa dominated by reed canarygrass was monitored hourly. Differences between water level measurements taken each hour were averaged to determine the hourly water table change in each month. Using these estimates of water use, the ALMANAC model was then calibrated to simulate plant transpiration values close to these water table use rates. Average monthly calculated daily plant water use rates were 3.3 mm d-1 in July and 2.3-2.8 mm d-1 in May, June, August, and September. Simulated bimonthly values for measured water use and plant transpiration simulated by the ALMANAC model differed by 14% or less. From May to October the mean ratio of measured to simulated values was 94%. Thus, the similarity between simulated plant transpiration and water use from the water table showed promise that this process-based model can realistically simulate water use under such grassland systems. ?? 2007 Elsevier B.V. All rights reserved.

Journal of Hydrology↗
USGS2013

Export of dissolved organic carbon from the Penobscot River basin in north-central Maine

Dissolved organic carbon (DOC) flux from the Penobscot River and its major tributaries in Maine was determined using continuous discharge measurements, discrete water sampling, and the LOADEST regression software. The average daily flux during 2004–2007 was 71 kg C ha −1 yr −1 (392 Mt C d −1 ), an amount larger than measured in most northern temperate and boreal rivers. Distinct seasonal variation was observed in the relation between concentration and discharge (C–Q). During June through December (summer/fall), there was a relatively steep positive C–Q relation where concentration increased by a factor of 2–3 over the approximately 20-fold range of observed stream discharge for the Penobscot River near Eddington, Maine. In contrast, during January through May (winter/spring), DOC concentration did not increase with increasing discharge. In addition, we observed a major shift in the C–Q between 2004–2005 and 2006–2007, apparently resulting from unprecedented rainfall, runoff, and soil flushing beginning in late fall 2005. The relative contribution to the total Penobscot River basin DOC flux from each tributary varied dramatically by season, reflecting the role of large regulated reservoirs in certain basins. DOC concentration and flux per unit watershed area were highest in tributaries containing the largest areas in palustrine wetlands. Tributary DOC concentration and flux was positively correlated to percentage wetland area. Climatic or environmental changes that influence the magnitude or timing of river discharge or the abundance of wetlands will likely affect the export of DOC to the near-coastal ocean.

Maine↗
USGS2013

Increases in flood magnitudes in California under warming climates

Downscaled and hydrologically modeled projections from an ensemble of 16 Global Climate Models suggest that flooding may become more intense on the western slopes of the Sierra Nevada mountains, the primary source for California’s managed water system. By the end of the 21st century, all 16 climate projections for the high greenhouse-gas emission SRES A2 scenario yield larger floods with return periods ranging 2–50 years for both the Northern Sierra Nevada and Southern Sierra Nevada, regardless of the direction of change in mean precipitation. By end of century, discharges from the Northern Sierra Nevada with 50-year return periods increase by 30–90% depending on climate model, compared to historical values. Corresponding flood flows from the Southern Sierra increase by 50–100%. The increases in simulated 50 year flood flows are larger (at 95% confidence level) than would be expected due to natural variability by as early as 2035 for the SRES A2 scenario.

California↗
USGS2015

Groundwater recharge assessment at local and episodic scale in a soil mantled perched karst aquifer in southern Italy

Groundwater recharge assessment of karst aquifers, at various spatial and temporal scales, is a major scientific topic of current importance, since these aquifers play an essential role for both socio-economic development and fluvial ecosystems. In this study, groundwater recharge was estimated at local and episodic scales in a representative perched karst aquifer in a region of southern Italy with a Mediterranean climate. The research utilized measurements of precipitation, air temperature, soil water content, and water-table depth, obtained in 2008 at the Acqua della Madonna test area (Terminio Mount karst aquifer, Campania region). At this location the aquifer is overlain by ash-fall pyroclastic soils. The Episodic Master Recession (EMR) method, an improved version of the Water Table Fluctuation (WTF) method, was applied to estimate the amount of recharge generated episodically by individual rainfall events. The method also quantifies the amount of precipitation generating each recharge episode, thus permitting calculation of the Recharge to the Precipitation Ratio (RPR) on a storm-by-storm basis. Depending on the seasonally varying air temperature, evapotranspiration, and precipitation patterns, calculated values of RPR varied between 35% and 97% among the individual episodes. A multiple linear correlation of the RPR with both the average intensity of recharging rainfall events and the antecedent soil water content was calculated. Given the relatively easy measurability of precipitation and soil water content, such an empirical model would have great hydrogeological and practical utility. It would facilitate short-term forecasting of recharge in karst aquifers of the Mediterranean region and other aquifers with similar hydrogeological characteristics. By establishing relationships between the RPR and climate-dependent variables such as average storm intensity, it would facilitate prediction of climate-change effects on groundwater recharge. The EMR methodology could further be applied to other aquifers for evaluating the relationship of recharge to various hydrometeorological and hydrogeological processes.

Journal of Hydrology↗
USGS2019

Evidence for conservative transport of dissolved organic carbon in major river basins in the Gulf of Maine Watershed

Transport and fate of dissolved organic carbon (DOC) in rivers are important aspects of the carbon cycle and the critical linkage between terrestrial, aquatic, and marine systems. Recent studies have quantified fluvial export to the marine environment in many systems, but in-stream losses of DOC are poorly constrained. This study compares DOC yields (kg C/ha) between the area-weighted averages of several tributaries within larger watersheds with the DOC yields of the larger watersheds to gain insight on in-stream losses in larger river systems. Four large watersheds, 22 tributaries to those watersheds, and 5 additional main stem locations in Maine were studied during 1 April to 15 November in 2011 through 2013. There were no significant differences in the area-weighted average DOC yield of the tributaries and the larger watersheds indicating little net in-stream loss in the main stems of the larger rivers. It is unlikely that inputs of DOC from un-gauged areas compensated for losses from gauged tributaries based on similarity in DOC yield longitudinally along the main stems of two of the rivers. In addition, wetland abundance, which is associated with higher DOC yield in this environment, did not consistently increase from tributaries to the larger watershed or longitudinally along the main stems. This geographic distribution of wetlands therefore also indicates that it is unlikely that inputs of DOC from un-gauged areas compensated for losses from gauged tributaries. These findings suggest that in-stream losses of DOC in these larger river systems are minimal and that the vast majority of DOC in major rivers in Maine is transported conservatively to the coastal ocean.

Maine↗
USGS2021

Summer runoff generation in foothill catchments of the Colorado Front Range

Climatic shifts, disturbances, and land-use change can alter hydrologic flowpaths, water quality, and water supply to downstream communities. Prior research investigating streamflow generation processes in mountainous areas has largely focused on high-elevation alpine and subalpine catchments; less is known about these processes in lower-elevation foothills and montane catchments. In these lower-elevation ecoregions, precipitation shifts seasonally from snow to rain, which can result in differing seasonal flowpaths. We analyzed stream water for electrical conductivity, SiO 2 , Ca, Mg, Na, Cl, SO 4 , K, and dissolved organic carbon on both a weekly and storm event basis from April to August 2018 in three small (<10 km 2 ) foothill catchments, and one larger (63.2 km 2 ) catchment extending from the foothills to the subalpine ecoregions, in the Colorado Front Range. Using two end-member hydrograph separations and concentration-runoff relationships, we inferred the dominant catchment-scale flowpaths of precipitation to the streams. We selected catchments with varying land use to investigate the relationship between these characteristics and hydrologic flowpaths. We observed that concentrations of lithogenic constituents generally increased and dissolved organic carbon decreased as seasonal runoff decreased in the three foothill catchments, reflecting a transition from shallow subsurface flowpaths to deeper subsurface flowpaths. Elevated SO 4 and Cl concentrations during low-flow periods in two of our catchments suggest that historical or current anthropogenic activities, such as mining, application of road salt, and/or near-stream septic systems, affect local stream and groundwater chemistry. In a foothill catchment with anthropogenic and geologic impervious surfaces, streamflow during storm responses was sourced from faster, surficial flowpaths compared to a less disturbed neighboring catchment, highlighting the influence of anthropogenic land-use on runoff generation. This study provides insight into the fundamental hydrology of foothill catchments and how they may function in the future with human development, precipitation shifts and disturbances.

Colorado↗
USGS2021

The influence of land cover and storm magnitude on hydrologic flowpath activation and runoff generation in steep tropical catchments of central Panama

Despite abundant research documenting that land use/land cover (LULC) have substantial impacts on the hydrology of humid tropical systems, field-based evidence for the physical mechanisms behind these impacts are still lacking. In particular, our understanding of the hydrologic flowpaths that generate runoff in these systems, and how they vary with respect to LULC is insufficient to inform both physically-based hydrologic modeling and land-use decision-making. In this study, we use end-member mixing analysis (EMMA) of stream chemistry, and hydrometric characterizations of hillslope soil moisture to identify hydrologic flowpaths in humid tropical steep-land catchments of varying LULC: mature tropical forest, young secondary tropical forest, cattle pasture. EMMA was applied to data from 14 storm events (six at the mature forest, five at the young secondary forest, and three at the cattle pasture) that were intensively sampled during the 2017 wet season representing a wide range of rainfall magnitudes and intensities. Additionally, volumetric-soil-moisture responses at multiple depths were characterized during and after 74 storm events occurring from 2015 to 2017. EMMA results indicated that lateral preferential flow within the top 30 cm of the soil profile was a dominant source of runoff generation at the two forested catchments, with the contribution of this flow path increasing with rainfall magnitude and intensity. This was corroborated by volumetric-soil-moisture data, that showed that a perched zone of saturation developed at 30 cm at the time of peak storm runoff during the largest events and lasted for the remaining duration of the event. EMMA indicated that runoff was a combination of infiltration-excess overland flow and lateral subsurface flow in the actively grazed pastoral catchment. There, overland flow contributed 62 % of runoff during the highest runoff rate sampled (35.3 mm/hr) and this contribution increased substantially with storm magnitude. This flowpath identification was also supported by volumetric-soil-moisture data at the pasture, with peak saturation at all depths during the largest storm events occurring up to 30 min after peak runoff. These results provide a mechanistic explanation for previously observed hydrologic differences among tropical LULCs. Additionally, the wide range of hydrologic conditions during these storm events provide a basis for understanding how future changes to this, and similar humid tropical regions will impact hydrological processes and water availability.

Journal of Hydrology↗
USGS2026

Drone–based camera discharge derived from the entropy–based probability concept and image velocimetry algorithms

In June and August 2023, the U.S. Geological Survey (USGS) conducted small Uncrewed Aircraft System (sUAS or drone) flights at five streamgages in the Upper Colorado River Basin, Colorado, USA. The objectives of this research were (1) compare maximum surface velocities and discharges derived from Space-time Image Velocimetry (STIV) and Particle Image Velocimetry (PIV) and (2) evaluate drone-based camera algorithms that rely on the entropy-based probability concept and mid-section method relative to standard discharge measurements. To evaluate the scalability and transferability of the methods, river reaches were selected with varying top widths, depths, and discharges and colocated with existing USGS streamgages. Percent errors between drone-based camera algorithms and hydroacoustic measurements were substantially smaller for large rivers such as the Colorado River, Colorado when compared to small rivers such as the Blue River, Colorado. A Wilcoxon signed-rank test suggests there was no difference between the discharges generated by STIV and PIV algorithms and hydroacoustic measurements. This implies that, if the appropriate algorithms are properly parameterized, drone-based camera discharges are consistent with standard discharge measurements when siting is conducted appropriately.

Colorado↗
USGS2008

Collaboration tools and techniques for large model datasets

In MREA and many other marine applications, it is common to have multiple models running with different grids, run by different institutions. Techniques and tools are described for low-bandwidth delivery of data from large multidimensional datasets, such as those from meteorological and oceanographic models, directly into generic analysis and visualization tools. Output is stored using the NetCDF CF Metadata Conventions, and then delivered to collaborators over the web via OPeNDAP. OPeNDAP datasets served by different institutions are then organized via THREDDS catalogs. Tools and procedures are then used which enable scientists to explore data on the original model grids using tools they are familiar with. It is also low-bandwidth, enabling users to extract just the data they require, an important feature for access from ship or remote areas. The entire implementation is simple enough to be handled by modelers working with their webmasters - no advanced programming support is necessary. ?? 2007 Elsevier B.V. All rights reserved.

Journal of Marine Systems↗
USGS2008

Surface drift prediction in the Adriatic Sea using hyper-ensemble statistics on atmospheric, ocean and wave models: Uncertainties and probability distribution areas

Despite numerous and regular improvements in underlying models, surface drift prediction in the ocean remains a challenging task because of our yet limited understanding of all processes involved. Hence, deterministic approaches to the problem are often limited by empirical assumptions on underlying physics. Multi-model hyper-ensemble forecasts, which exploit the power of an optimal local combination of available information including ocean, atmospheric and wave models, may show superior forecasting skills when compared to individual models because they allow for local correction and/or bias removal. In this work, we explore in greater detail the potential and limitations of the hyper-ensemble method in the Adriatic Sea, using a comprehensive surface drifter database. The performance of the hyper-ensembles and the individual models are discussed by analyzing associated uncertainties and probability distribution maps. Results suggest that the stochastic method may reduce position errors significantly for 12 to 72??h forecasts and hence compete with pure deterministic approaches. ?? 2007 NATO Undersea Research Centre (NURC).

Journal of Marine Systems↗
USGS2010

Predicting potentially toxigenic Pseudo-nitzschia blooms in the Chesapeake Bay

Harmful algal blooms are now recognized as a significant threat to the Chesapeake Bay as they can severely compromise the economic viability of important recreational and commercial fisheries in the largest estuary of the United States. This study describes the development of empirical models for the potentially domoic acid-producing Pseudo-nitzschia species complex present in the Bay, developed from a 22-year time series of cell abundance and concurrent measurements of hydrographic and chemical properties. Using a logistic Generalized Linear Model (GLM) approach, model parameters and performance were compared over a range of Pseudo-nitzschia bloom thresholds relevant to toxin production by different species. Small-threshold blooms (???10cellsmL-1) are explained by time of year, location, and variability in surface values of phosphate, temperature, nitrate plus nitrite, and freshwater discharge. Medium- (100cellsmL-1) to large- threshold (1000cellsmL-1) blooms are further explained by salinity, silicic acid, dissolved organic carbon, and light attenuation (Secchi) depth. These predictors are similar to other models for Pseudo-nitzschia blooms on the west coast, suggesting commonalities across ecosystems. Hindcasts of bloom probabilities at a 19% bloom prediction point yield a Heidke Skill Score of -53%, a Probability of Detection ~75%, a False Alarm Ratio of ~52%, and a Probability of False Detection ~9%. The implication of possible future changes in Baywide nutrient stoichiometry on Pseudo-nitzschia blooms is discussed. ?? 2010 Elsevier B.V.

Journal of Marine Systems↗
USGS2018

The geochemistry of loess: Asian and North American deposits compared

Loess is widely distributed over Asia and North America and constitutes one of the most important surficial deposits that serve as terrestrial records of the Quaternary. The oldest Pleistocene loess in China is likely ∼2.6 Ma, thus spanning much or all of the Pleistocene. In North America, most loess is no older than the penultimate glacial period, with the exception of Alaska, where the record may go back to ∼3.0 Ma. On both continents, loess deposits date primarily to glacial periods, and interglacial or interstadial periods are represented by paleosols. Both glacial and non-glacial sources of silts that comprise the bulk of loess deposits are found on both continents. Although loess has been considered to be representative of the average upper continental crust, there are regionally distinctive compositions of loess in both Asia and North America. Loess deposits in Asia from Yakutia, Tajikistan, and China have compositionally distinct major element compositions, due to varying abundances of silicate minerals, carbonate minerals, and clay minerals. In North America, loess in the Mississippi River valley, the Great Plains, and Alaska are also distinguishable with regard to major element composition that reflects highly diverse source sediments. Trace element geochemistry (Sc-Th-Zr and the rare earth elements) also shows regional diversity of loess bodies, in both Asia and North America. On both continents, most loess bodies show significant contributions from later-cycle, altered sedimentary rocks, as opposed to direct derivation from igneous rocks. Further, some loess bodies have detectable contributions from mafic igneous rocks as well as major contributions from average, upper-crustal, felsic rocks. Intercalated paleosols in loess sections show geochemical compositions that differ significantly from the underlying loess parent materials. Ratios of soluble-to-insoluble elements show depletions in paleosols due to chemical weathering losses of calcite, dolomite, plagioclase, mica, apatite, and smectite. In Asia and North America, the last interglacial paleosol is more weathered than equivalent modern soils, which could be due either to a climate that was warmer and more humid, a longer period of pedogenesis, or both. In Asia, early Pleistocene loess and paleosols are both more weathered than those from the middle and late Pleistocene, forming prior to a mid-Pleistocene aridification of Asia from uplift of the Tibetan Plateau. Understanding the geochemistry of loess and paleosols can tell us much about past atmospheric circulation, past temperature and moisture regimes, and even tectonic processes.

Journal of Asian Earth Sciences↗
USGS2010

Interpretation and analysis of planetary structures

Structural geology is an integral part of planetary science. Planetary structures provide the framework for determining the character and sequence of crustal deformation while simultaneously establishing the observational basis required to test geodynamic hypotheses for the deformation of planetary and satellite lithospheres. The availability of datasets that record spatial and topographic information with a resolution that matches or, in many cases, exceeds, what is available for Earth-based studies permits the deformation of several planets and satellites to be investigated down to the local or outcrop scales. The geometry and kinematics of common planetary structures such as joints, igneous dikes, deformation bands, faults, and folds can be determined with confidence from their distinctive morphologic and topographic signatures, enabling the structural histories and deformation magnitudes to be determined. Segmentation, displacement profiles, relay ramps, footwall anticlines, displacement-controlled depocenters, and other well-known characteristics of terrestrial normal fault and graben systems reveal the sequence and processes of fault growth in numerous planetary examples. Systems of thrust faults having both blind and surface-breaking components are important elements on several bodies including Mercury, the Moon, and Mars. Strike-slip faults have been identified on bodies including Mars and Europa with oblique extension found on Ganymede. Using field-based studies of Earth-based structures as a guide, planetary structures provide a means to explore and evaluate the causative stresses. Despite the wide range in structural styles across the solar system, plate tectonics is recognized only on the Earth, with the other planets and satellites deforming in the absence of large-scale horizontal motions and attendant plate recycling.

Journal of Structural Geology↗
USGS2012

Observed source parameters for dynamic rupture with non-uniform initial stressand relatively high fracture energy

We have conducted dynamic rupture propagation experiments to establish the relations between in-source stress drop, fracture energy and the resulting particle velocity during slip of an unconfined 2 m long laboratory fault at normal stresses between 4 and 8 MPa. To produce high fracture energy in the source we use a rough fault that has a large slip weakening distance. An artifact of the high fracture energy is that the nucleation zone is large such that precursory slip reduces fault strength over a large fraction of the total fault length prior to dynamic rupture, making the initial stress non-uniform. Shear stress, particle velocity, fault slip and acceleration were recorded coseismically at multiple locations along strike and at small fault-normal distances. Stress drop increases weakly with normal stress. Average slip rate depends linearly on the fault strength loss and on static stress drop, both with a nonzero intercept. A minimum fracture energy of 1.8 J/m 2 and a linear slip weakening distance of 33 μm are inferred from the intercept. The large slip weakening distance also affects the average slip rate which is reduced by in-source energy dissipation from on-fault fracture energy. Because of the low normal stress and small per event slip (∼86 μm), no thermal weakening such as melting or pore fluid pressurization occurs in these experiments. Despite the relatively high fracture energy, and the very low heat production, energy partitioning during these laboratory earthquakes is very similar to typical earthquake source properties. The product of fracture energy and fault area is larger than the radiated energy. Seismic efficiency is low at ∼2%. The ratio of apparent stress to static stress drop is ∼27%, consistent with measured overshoot. The fracture efficiency is ∼33%. The static and dynamic stress drops when extrapolated to crustal stresses are 2–7.3 MPa and in the range of typical earthquake stress drops. As the relatively high fracture energy reduces the slip velocities in these experiments, the extrapolated average particle velocities for crustal stresses are 0.18–0.6 m/s. That these experiments are consistent with typical earthquake source properties suggests, albeit indirectly, that thermal weakening mechanisms such as thermal pressurization and melting which lead to near complete stress drops, dominate earthquake source properties only for exceptional events unless crustal stresses are low.

Journal of Structural Geology↗
USGS2018

Frictional properties and 3-D stress analysis of the southern Alpine Fault, New Zealand

New Zealand's Alpine Fault (AF) ruptures quasi-periodically in large-magnitude earthquakes. Paleoseismological evidence suggests that about half of all recognized AF earthquakes terminated at the boundary between the Central and South Westland sections of the fault. There, fault geometry and the polarity of uplift change. The South Westland AF exhibits oblique-normal fault motion on a structure oriented 052°/82°SE that, for at least 35 km along strike, contains saponite-rich principal slip zone gouges. New hydrothermal friction experiments reveal that the saponite fault gouge is frictionally weak, exhibiting friction coefficients between μ = 0.12 and μ = 0.16 for a range of temperatures ( T = 25–210 °C) and effective normal stresses ( σ n ' = 31.2–93.6 MPa). The saponite gouge is rate-strengthening in all velocity steps performed at velocities between 0.01 and 3.0 μm/s, behavior conducive to aseismic creep. A three-dimensional stress analysis shows that the South Westland AF is favorably oriented with respect to the regional stress field for slip within the frictionally weak saponite fault gouge. Geometrically, the fault is severely misoriented for slip in any fault-forming materials with friction coefficients exceeding μ ∼0.5. The combination of weak gouges prone to aseismic creep, strong asperities , and low resolved shear stress may impede earthquake rupture propagation along the South Westland Alpine Fault.

Journal of Structural Geology↗
USGS2006

Strand-specific, real-time RT-PCR assays for quantification of genomic and positive-sense RNAs of the fish rhabdovirus, Infectious hematopoietic necrosis virus

The fish rhabdovirus, Infectious hematopoietic necrosis virus (IHNV), is an important pathogen of salmonids. Cell culture assays have traditionally been used to quantify levels of IHNV in samples; however, real-time or quantitative RT-PCR assays have been proposed as a rapid alternative. For viruses having a single-stranded, negative-sense RNA genome, standard qRT-PCR assays do not distinguish between the negative-sense genome and positive-sense RNA species including mRNA and anti-genome. Thus, these methods do not determine viral genome copy number. This study reports development of strand-specific, qRT-PCR assays that use tagged primers for enhancing strand specificity during cDNA synthesis and quantitative PCR. Protocols were developed for positive-strand specific (pss-qRT-PCR) and negative-strand specific (nss-qRT-PCR) assays for IHNV glycoprotein (G) gene sequences. Validation with synthetic RNA transcripts demonstrated the assays could discriminate the correct strand with greater than 1000-fold fidelity. The number of genome copies in livers of IHNV-infected fish determined by nss-qRT-PCR was, on average, 8000-fold greater than the number of infectious units as determined by plaque assay. We also compared the number of genome copies with the quantity of positive-sense RNA and determined that the ratio of positive-sense molecules to negative-sense genome copies was, on average, 2.7:1. Potential future applications of these IHNV strand-specific qRT-PCR assays are discussed.

Journal of Virological Methods↗