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Evaluating sources of bias in pedigree-based estimates of breeding population size

Applications of genetic-based estimates of population size are expanding, especially for species for which traditional demographic estimation methods are intractable due to the rarity of adult encounters. Estimates of breeding population size ( N S ) are particularly amenable to genetic-based approaches as the parameter can be estimated using pedigrees reconstructed from genetic data gathered from discrete juvenile cohorts, therefore eliminating the need to sample adults in the population. However, a critical evaluation of how genotyping and sampling effort influence bias in pedigree reconstruction, and how these biases subsequently influence estimates of N S , is needed to evaluate the efficacy of the approach under a range of scenarios. We simulated a model system to understand the interactive effects of genotyping and sampling effort on error in genetic pedigrees reconstructed from the program COLONY . We then evaluated how errors in pedigree reconstruction influenced bias and precision in estimates of N S using three different rarefaction estimators. Results indicated that pedigree error can be minimal when adequate genetic data are available, such as when juvenile sample sizes are large and/or individuals are genotyped at many informative loci. However, even in cases for which data are limited, using results of the simulation analysis to understand the magnitude and sources of bias in reconstructed pedigrees can still be informative when estimating N S . We applied results of the simulation analysis to evaluate Nˆ S for a population of federally endangered Atlantic sturgeon ( Acipenser oxyrinchus oxyrinchus ) in the Delaware River, USA. Our results indicated that N S is likely to be three orders of magnitude lower compared with historic breeding population sizes, which is a considerable advancement in our understanding of the population status of Atlantic sturgeon in the Delaware River. Our analyses are broadly applicable in the design and interpretation of studies seeking to estimate N S and can help to guide conservation decisions when ecological uncertainty is high. The utility of these results is expected to grow as rapid advances in genetic technologies increase the popularity of genetic population monitoring and estimation.

Delaware, New Jersey, Pennsylvania↗

Using neutral landscape models to evaluate the umbrella species concept in an ecotone

Context Steep declines in North American rangeland biodiversity have prompted researchers and managers to use umbrella species as a tool to manage diverse suites of co-occurring wildlife, but efficacy of this method has been variable. Evaluation of prairie and shrubland grouse as umbrellas is typically restricted to observed overlap between umbrella and background species, but this approach does not distinguish between overlap due to ubiquity or niche overlap. Objectives We demonstrate a novel application of neutral landscape models (NLMs) to test the effectiveness of greater sage-grouse ( Centrocercus urophasianus ) as an umbrella species for grassland songbirds at a grassland-sagebrush ecotone in northeastern Wyoming, USA. Methods We leveraged existing spatial data representing sage-grouse habitat in two distinct seasons (nesting and late brood-rearing) and density and distribution of eight grassland songbirds. We applied a permutation-based analysis using NLMs to determine whether overlap between background species and greater sage-grouse was greater than expected by chance. Results Three species (western meadowlark Sturnella neglecta , loggerhead shrike Lanius ludovicianus , and lark bunting Calamospiza melanocorys ) had greater overlap than expected with at least one type of greater sage-grouse habitat, while western kingbirds ( Tyrannus verticalis ) indicated avoidance of all sage-grouse habitat assessed. Conclusions NLMs provided a more nuanced evaluation of the umbrella species concept than previously available and allowed us to differentiate between overlap due to ubiquity (e.g., vesper sparrow; Pooecetes gramineus ) rather than overlap in habitat use. All grassland passerine species with greater than expected overlap with sage-grouse habitat either nest in sagebrush (loggerhead shrike) or often select nest locations underneath small shrubs (western meadowlark, lark bunting). These results indicate that nesting substrate is a potential niche axis to consider when evaluating the umbrella species concept, especially within sagebrush-grassland ecotones.

Wyoming↗

A novel approach for next generation water use mapping using Landsat and Sentinel-2 satellite data

Evapotranspiration (ET) is needed in a range of applications in hydrology, climatology, ecology, and agriculture. Remote sensing-based estimation is the only viable and economical method for ET estimation over large areas. The current Landsat satellites provide images every 16 days limiting the ability to capture biophysical changes affecting ET. Thus, we explored the potential integration of Landsat 8 and Sentinel-2 data for estimating ET using a surface energy balance model. The results indicate the proposed Landsat-Sentinel data fusion approach substantially reduced relative errors from 48% to 10% on area-wide and from 49% to 17% on pixel-wide compared to linear interpolation between two Landsat images. The proposed approach had a better agreement with expected actual ET maps across high-vegetation conditions than in low-vegetation conditions. The finer temporal resolution and better accuracy of ET maps based on Landsat-Sentinel integration is of great importance in managing limited water resources.

Arizona, California↗

The cost of fear

What should parents do when they detect indications of more predators nearby that might eat their babies? This scenario is commonly faced by parents in the wild, and the consequences are important. The number of offspring that organisms produce has a major influence on fitness and, when averaged across a population, affects whether this population will increase or decrease. Offspring production thus has critical implications for evolution via fitness, and ecology and conservation via demography. On page 1398 of this issue, Zanette et al. ( 1 ) show that the fear of predation can, by itself, strongly affect the number of offspring produced over an annual cycle by song sparrows (see the figure).

Science↗

Habitat suitability and conservation of the Giant Gartersnake (Thamnophis gigas) in the Sacramento Valley of California

Resource managers often have little information regarding the habitat requirements and distribution of rare species. Factor analysis-based habitat suitability models describe the ecological niche of a species and identify locations where these conditions occur on the landscape using existing occurrence data. We used factor analyses to assess the suitability of habitats for Thamnophis gigas (Giant Gartersnake), a rare, threatened species endemic to the Central Valley of California, USA, and to map the locations of habitat suitable for T. gigas in the Sacramento Valley. Factor analyses indicated that the niche of T. gigas is composed of sites near rice agriculture with low stream densities. Sites with high canal densities and near wetlands also appeared suitable, but results for these variables were sensitive to potential sampling bias. In the Sacramento Valley, suitable habitats occur primarily in the central portion of the valley floor. Based upon the results of the factor analyses, recovery planning for T. gigas will require an on-the-ground assessment of the current distribution and abundance of T. gigas, maintaining the few remaining natural wetlands and the practice of rice agriculture in the Sacramento Valley, and studying the effects of agricultural practices and land use changes on populations of T. gigas. ?? 2010 by the American Society of Ichthyologists and Herpetologists.

Copeia↗

Development of a decision support tool for water and resource management using biotic, abiotic, and hydrological assessments of Topock Marsh, Arizona

Topock Marsh is a large wetland adjacent to the Colorado River and the main feature of Havasu National Wildlife Refuge (Havasu NWR) in southern Arizona. In 2010, the U.S. Fish and Wildlife Service (FWS) and Bureau of Reclamation began a project to improve water management capabilities at Topock Marsh and protect habitats and species. Initial construction required a drawdown, which caused below-average inflows and water depths in 2010–11. U.S. Geological Survey Fort Collins Science Center (FORT) scientists collected an assemblage of biotic, abiotic, and hydrologic data from Topock Marsh during the drawdown and immediately after, thus obtaining valuable information needed by FWS. Building upon that work, FORT developed a decision support system (DSS) to better understand ecosystem health and function of Topock Marsh under various hydrologic conditions. The DSS was developed using a spatially explicit geographic information system package of historical data, habitat indices, and analytical tools to synthesize outputs for hydrologic time periods. Deliverables include high-resolution orthorectified imagery of Topock Marsh; a DSS tool that can be used by Havasu NWR to compare habitat availability associated with three hydrologic scenarios (dry, average, wet years); and this final report which details study results. This project, therefore, has addressed critical FWS management questions by integrating ecologic and hydrologic information into a DSS framework. This DSS will assist refuge management to make better informed decisions about refuge operations and better understand the ecological results of those decisions by providing tools to identify the effects of water operations on species-specific habitat and ecological processes. While this approach was developed to help FWS use the best available science to determine more effective water management strategies at Havasu NWR, technologies used in this study could be applied elsewhere within the region.

Arizona↗

Sources of mercury in sediments, water, and fish of the lakes of Whatcom County, Washington

Concerns about mercury (Hg) contamination in Lake Whatcom, Washington, were raised in the late 1990s after a watershed protection survey reported elevated concentrations of Hg in smallmouth bass. The U.S. Geological Survey, the Whatcom County Health Department, and the Washington State Department of Ecology (Ecology) cooperated to develop a study to review existing data and collect new data that would lead to a better understanding of Hg deposition to Lake Whatcom and other lakes in Whatcom County, Washington. A simple atmospheric deposition model was developed that allowed comparisons of the deposition of Hg to the surfaces of each lake. Estimates of Hg deposition derived from the model indicated that the most significant deposition of Hg would have occurred to the lakes north of the City of Bellingham. These lakes were in the primary wind pattern of two municipal waste incinerators. Of all the lakes examined, basin 1 of Lake Whatcom would have been most affected by the Hg emissions from the chlor-alkali plant and the municipal sewage-sludge incinerator in the City of Bellingham. The length-adjusted concentrations of Hg in largemouth and smallmouth bass were not related to estimated deposition rates of Hg to the lakes from local atmospheric sources. Total Hg concentrations in the surface sediments of Lake Whatcom are affected by the sedimentation of fine-grained particles, whereas organic carbon regulates the concentration of methyl-Hg in the surface sediments of the lake. Hg concentrations in dated sediment core samples indicate that increases in Hg sedimentation were largest during the first half of the 20th century. Increases in Hg sedimentation were smaller after the chlor-alkali plant and the incinerators began operating between 1964 and 1984. Analysis of sediments recently deposited in basin 1 of Lake Whatcom, Lake Terrell, and Lake Samish indicates a decrease in Hg sedimentation. Concentrations of Hg in Seattle precipitation and in tributary waters were used to calculate current (2002-03) loadings of Hg to Lake Whatcom. Hg in tributaries contributed 59 percent of the total Hg, whereas non-local atmospheric deposition was estimated to have contributed 41 percent of the 303 grams of Hg entering Lake Whatcom each year. However, these inputs cannot be verified without a better understanding of the sources of sediment to Lake Whatcom.

Scientific Investigations Report↗

The gold tegu, Tupinambis teguixin (Linnaeus, 1758) sensu lato (Squamata: Teiidae): evidence for an established population in Florida

Gold tegus, Tupinambis teguixin (Linnaeus, 1758), are generalist predators from South America and are ecologically similar to Argentine black and white tegus ( Salvator merianae ), a successful invader in Florida. We trapped gold tegus in Broward and Miami-Dade counties, Florida, USA. In Miami-Dade County, collection occurred from 2008 through 2016. We combined new trapping records with previous literature records. Ten gold tegus of both sexes and multiple size classes over a nine year period indicate a reproducing population in Miami-Dade County. Tupinambis teguixin is the sixth established non-native teiid species in Florida. Additionally, we report Tupinambis teguixin from Sarasota and Lee counties, Florida, USA. Determining population status in those counties requires further study. It is critical to differentiate this species from Salvator merianae during removal efforts. The possibility of eradicating Tupinambis teguixin exists if proper identification enables reliable monitoring of the populations.

Florida↗

Measures of fish behavior as indicators of sublethal toxicosis during standard toxicity tests

Behavioral functions essential for growth and survival can be dramatically altered by sublethal exposure to toxicants. Measures of these behavioral responses are effective in detecting adverse effects of sublethal contaminant exposure. Behavioral responses of fishes can be qualitatively and quantitatively evaluated during routine toxicity tests. At selected intervals of exposure, qualitative evaluations are accomplished through direct observations, whereas video recordings are used for quantitative evaluations. Standardized procedures for behavioral evaluation are readily applicable to different fish species and provide rapid, sensitive, and ecologically relevant assessments of sublethal exposure. The methods are readily applied to standardized test protocols.

Book chapter↗

Distribution probability of the Virginia northern flying squirrel in the High Allegheny Mountains

In the central Appalachians of Virginia and West Virginia, the Virginia northern flying squirrel ( Glaucomys sabrinus fuscus ; VNFS) is a subspecies of northern flying squirrel generally associated with red spruce ( Picea rubens )-dominated forests at high elevations. Listed as endangered by the U.S. Fish and Wildlife Service from 1985 to 2013, the VNFS currently is the subject of a 10-year post-delisting assessment. Still considered a state-listed species in Virginia and a species of greatest conservation need in West Virginia, the VNFS serves as a focal target for red spruce restoration activities in the High Allegheny Region (HAR) of the two states. Owing to the cryptic nature of VNFS and its low detection probability in live-capture surveys, managers in the region rely on habitat models to assess probable presence. Using long-term nest-box, live-trapping, and radio-telemetry data matched with updated high elevation forest-type coverage data for the region, we created a new VNFS resource selection function and spatial coverage map. Inputting red spruce cover, increasing elevation, and decreasing landform index (increasing site shelteredness) composed the best model explaining VNFS occurrence. The calculated amount of low-quality habitat was congruent with previous modeling efforts; however, inclusion of more VNFS occurrence records in the current effort indicated that previous efforts substantially underestimated the amount (>400%) of extant high quality VNFS habitat. We estimate the HAR to contain approximately 197,952 ha with ≥0.50 predicted probability of occurrence of VNFS. In addition to potentially improving current and future VNFS live-capture surveys, with this model managers may better target forests for red spruce restoration to increase high elevation forest ecological integrity and to improve habitat patch connectedness for VNFS.

Maryland, Virginia, West Virginia↗

Increasing connectivity between metapopulation ecology and landscape ecology

Metapopulation ecology and landscape ecology aim to understand how spatial structure influences ecological processes, yet these disciplines address the problem using fundamentally different modeling approaches. Metapopulation models describe how the spatial distribution of patches affects colonization and extinction, but often do not account for the heterogeneity in the landscape between patches. Models in landscape ecology use detailed descriptions of landscape structure, but often without considering colonization and extinction dynamics. We present a novel spatially explicit modeling framework for narrowing the divide between these disciplines to advance understanding of the effects of landscape structure on metapopulation dynamics. Unlike previous efforts, this framework allows for statistical inference on landscape resistance to colonization using empirical data. We demonstrate the approach using 11 yr of data on a threatened amphibian in a desert ecosystem. Occupancy data for Lithobates chiricahuensis (Chiricahua leopard frog) were collected on the Buenos Aires National Wildlife Refuge (BANWR), Arizona, USA from 2007 to 2017 following a reintroduction in 2003. Results indicated that colonization dynamics were influenced by both patch characteristics and landscape structure. Landscape resistance increased with increasing elevation and distance to the nearest streambed. Colonization rate was also influenced by patch quality, with semi‐permanent and permanent ponds contributing substantially more to the colonization of neighboring ponds relative to intermittent ponds. Ponds that only hold water intermittently also had the highest extinction rate. Our modeling framework can be widely applied to understand metapopulation dynamics in complex landscapes, particularly in systems in which the environment between habitat patches influences the colonization process

Ecology↗

Metagenomic analysis of planktonic microbial consortia from a non-tidal urban-impacted segment of James River

Knowledge of the diversity and ecological function of the microbial consortia of James River in Virginia, USA, is essential to developing a more complete understanding of the ecology of this model river system. Metagenomic analysis of James River's planktonic microbial community was performed for the first time using an unamplified genomic library and a 16S rDNA amplicon library prepared and sequenced by Ion PGM and MiSeq, respectively. From the 0.46-Gb WGS library (GenBank:SRR1146621; MG-RAST:4532156.3), 4 × 10 6 reads revealed >3 × 10 6 genes, 240 families of prokaryotes, and 155 families of eukaryotes. From the 0.68-Gb 16S library (GenBank:SRR2124995; MG-RAST:4631271.3; EMB:2184), 4 × 10 6 reads revealed 259 families of eubacteria. Results of the WGS and 16S analyses were highly consistent and indicated that more than half of the bacterial sequences were Proteobacteria , predominantly Comamonadaceae . The most numerous genera in this group were Acidovorax (including iron oxidizers, nitrotolulene degraders, and plant pathogens), which accounted for 10 % of assigned bacterial reads. Polaromonas were another 6 % of all bacterial reads, with many assignments to groups capable of degrading polycyclic aromatic hydrocarbons. Albidiferax (iron reducers) and Variovorax (biodegraders of a variety of natural biogenic compounds as well as anthropogenic contaminants such as polycyclic aromatic hydrocarbons and endocrine disruptors) each accounted for an additional 3 % of bacterial reads. Comparison of these data to other publically-available aquatic metagenomes revealed that this stretch of James River is highly similar to the upper Mississippi River, and that these river systems are more similar to aquaculture and sludge ecosystems than they are to lakes or to a pristine section of the upper Amazon River. Taken together, these analyses exposed previously unknown aspects of microbial biodiversity, documented the ecological responses of microbes to urban effects, and revealed the noteworthy presence of 22 human-pathogenic bacterial genera (e.g., Enterobacteriaceae , pathogenic Pseudomonadaceae , and ‘ Vibrionales' ) and 6 pathogenic eukaryotic genera (e.g., Trypanosomatidae and Vahlkampfiidae). This information about pathogen diversity may be used to promote human epidemiological studies, enhance existing water quality monitoring efforts, and increase awareness of the possible health risks associated with recreational use of James River.

Virginia↗

Fuel-reduction management alters plant composition, carbon and nitrogen pools, and soil thaw in Alaskan boreal forest

Increasing wildfire activity in Alaska's boreal forests has led to greater fuel-reduction management. Management has been implemented to reduce wildfire spread, but the ecological impacts of these practices are poorly known. We quantified the effects of hand-thinning and shearblading on above- and belowground stand characteristics, plant species composition, carbon (C) and nitrogen (N) pools, and soil thaw across 19 black spruce ( Picea mariana ) dominated sites in interior Alaska treated 2-12 years prior to sampling. The density of deciduous tree seedlings was significantly higher in shearbladed areas compared to unmanaged forest (6.4 vs. 0.1 stems m −2 ), and unmanaged stands exhibited the highest mean density of conifer seedlings and layers (1.4 stems m −2 ). Understory plant community composition was most similar between unmanaged and thinned stands. Shearblading resulted in a near complete loss of aboveground tree biomass C pools while thinning approximately halved the C pool size (1.2 kg C m −2 compared to 3.1 kg C m −2 in unmanaged forest). Significantly smaller soil organic layer (SOL) C and N pools were observed in shearbladed stands (3.2 kg C m −2 and 116.8 g N m −2 ) relative to thinned (6.0 kg C m −2 and 192.2 g N m −2 ) and unmanaged (5.9 kg C m −2 and 178.7 g N m −2 ) stands. No difference in C and N pool sizes in the uppermost 10 cm of mineral soil was observed among stand types. Total C stocks for measured pools was 2.6 kg C m −2 smaller in thinned stands and 5.8 kg C m −2 smaller in shearbladed stands when compared to unmanaged forest. Soil thaw depth averaged 13 cm deeper in thinned areas and 46 cm deeper in shearbladed areas relative to adjacent unmanaged stands, although variability was high across sites. Deeper soil thaw was linked to shallower SOL depth for unmanaged stands and both management types, however for any given SOL depth, thaw tended to be deeper in shearbladed areas compared to unmanaged forest. These findings indicate that fuel-reduction management alters plant community composition, C and N pools, and soil thaw depth, with consequences for ecosystem structure and function beyond those intended for fire management.

Alaska↗

Survey of invasive ants at Hakalau Forest National Wildlife Refuge

We conducted a survey for invasive ants at Hakalau Forest National Wildlife Refuge, Hawai‘i Island, during 2009–2010 to evaluate potential threats to native arthropod communities and food webs. The focal area of the survey was the upper portion of the Hakalau Unit of the refuge, where native forest was being restored in abandoned cattle pastures. This area, between 1575 and 1940 m elevations, contained much alien kikuyu grass (Pennisetum clandestinum), but koa (Acacia koa) trees and other native species that were planted in the past 20 years were rapidly filling in the pasture. We surveyed for ants at predetermined points along roads, fences, and corridors of planted koa. Sampling methods primarily consisted of hand searching and pitfall traps, but bait cards were used additionally in some instances. Our results indicated that a single species, Cardiocondyla kagutsuchi, was widespread across the upper portion of the refuge. Cardiocondyla kagutsuchi seemed absent, or at least rare, in areas of tall, dense grass. Due to the undulating topography of the area, however, the dense grass cover was interspersed with outcroppings of exposed, gravelly soil. Presumably due to warming by the sun, many of the outcropped habitats supported colonies of C. kagutsuchi. We did not detect ants in the old-growth forest below the abandoned pastures, presumably because microhabitat conditions under the forest canopy were unsuitable. Although ecological impacts of C. kagutsuchi have not been reported, they may be limited by the small size of the ant, the relatively small size of colonies, and the apparent preference of the ant for disturbed areas that are dominated by alien species. Notably, our survey of Keanakolu-Mana Road between the Observatory Road (John A. Burns Way) and the town of Waimea detected a population of Argentine ants (Linepithema humile) approximately 5.1 km north of the Maulua Section of the refuge. We also surveyed for ants on the Kona Forest Unit of the refuge. This small survey focused on approximately 14 km of roads located below about 1600 m elevation. We found two species, Solenopsis papuana and Nylanderia bourbonica. Solenopsis papuana was more widespread, being found along the southern, northern, and western boundaries, while N. bourbonica was detected only at 790 m elevation on the southern boundary. Of the two species, S. papuana seemed more likely to affect native arthropod communities due to its tendency to form relatively large, aggressive colonies and its ability to inhabit intact mesic and wet forests below 1100 m elevation. In contrast, the restriction of N. bourbonica to disturbed habitats indicated a reduced threat to native arthropod communities. Our results on the Kona Forest Unit corroborated those of a study conducted during 1999–2000, although the earlier study was more intensive over time and yielded small numbers of two additional species, Cardiocondyla wroughtonii and Tetramorium bicarinatum, both of which were detected below 792 m elevation along the southern boundary.

Hawaii↗

Quantifying variance across spatial scales as part of fire regime classifications

The emergence of large‐scale fire classifications and products informed by remote sensing data has enabled opportunities to include variability or heterogeneity as part of modern fire regime classifications. Currently, basic fire metrics such as mean fire return intervals are calculated without considering spatial variance in a management context. Fire return intervals are also only applicable at a particular grain size (defined as the spatial unit of interest) even though they are typically applied homogeneously. In this study, we utilized a 29‐yr fire occurrence database to show how spatial variance changes with respect to grain as postulated by Wiens ( 1989 ) when reporting fire patterns within the Great Plains, USA. We utilized data from the Monitoring Trends in Burn Severity database of fire occurrence for the years 1984–2012. We analyzed median numbers of fire along with their variance at four spatial grains ranging from small units (e.g., plots at 3 × 3 km resolution) to large units (e.g., landscapes at 1500 × 2700 km resolution). Median number of fire occurrences was consistently low, irrespective of grain. Despite the consistency in low median numbers of fires across grain, variance in the numbers of fires between units decreased. Variance within units, however, did not change as grain increased indicating fire‐pattern‐scale inconsistencies. Fire pattern interpretations depended entirely on the scale at which it is calculated. Given that the Great Plains region has a large disparity in fire patterns (i.e., some regions burn often, while others may never burn), fire regime classifications will benefit from including scale‐specific variance estimates as a foundation for understanding changes in fire regimes and corresponding social–ecological and policy responses.

Ecosphere↗

Changes in floral diversities, floral turnover rates, and climates in Campanian and Maastrichtian time, North Slope of Alaska

One-hundred-and-ten angiosperm pollen taxa have been found in upper Campanian to Masstrichtian rocks of the Colville River region, North Slope of Alaska. These are the highest paleolatitude Campanian and Maastrichtian floras known from North America. Total angiosperm pollen diversity rose during the Campanian and declined toward the end of the Maastrichtian. However, anemophilous porate pollen of the Betulaceae-Myricaceae-Ulmaceae complex increased gradually in diversity during the late Campanian and Maastrichtian and into the Paleocene. Turnover of angiosperm taxa was active throughout most of late Campanian and Maastrichtian time; rapid turnover affected mainly the taxa of zoophilous herbs, representing an bundant but ecologically subordinate element of the vegetation. Last appearances of pollen taxa during the late Campanian and Maastrichtian probably represented mainly extinctions rather than emigrations; end- Cretaceous angiosperm extinctions in the North American Arctic began well before the Cretaceous-Tertiary boundary event. The last appearances in the late Maastrichtian took place in bursts; they appear to represent stepwise rather than gradual events, which may indicate the existence of pulses of climatic change particularly in late Maastrichtian time. ?? 1989.

Alaska↗

Heritage strain and diet of wild young of year and yearling lake trout in the main basin of Lake Huron

Restoration of lake trout Salvelinus namaycush stocks in Lake Huron is a fish community objective developed to promote sustainable fish communities in the lake. Between 1985 and 2004, 12.65 million lake trout were stocked into Lake Huron representing eight different genetic strains. Collections of bona fide wild fish in USGS surveys have increased in recent years and this study examined the ancestry and diet of fish collected between 2004 and 2006 to explore the ecological role they occupy in Lake Huron. Analysis of microsatellite DNA revealed that both pure strain and inter-strain hybrids were observed, and the majority of fish were classified as Seneca Lake strain or Seneca Lake hybrids. Diets of 50 wild age-0 lake trout were examined. Mysis, chironomids, and zooplankton were common prey items of wild age-0 lake trout. These results indicate that stocked fish are successfully reproducing in Lake Huron indicating a level of restoration success. However, continued changes to the benthic macroinvertebrate community, particularly declines of Mysis, may limit growth and survival of wild fish and hinder restoration efforts.

Journal of Great Lakes Research↗

The fecal bacteria

The Fecal Bacteria offers a balanced, integrated discussion of fecal bacteria and their presence and ecology in the intestinal tract of mammals, in the environment, and in the food supply. This new volume covers their use in examining and assessing water quality in order to offer protection from illnesses related to swimming in or ingesting contaminated water, in addition to discussing their use in engineering considerations of water quality, modeling, monitoring, and regulations. Fecal bacteria are additionally used as indicators of contamination of ready-to-eat foods and fresh produce. The intestinal environment, the microbial community structure of the gut microbiota, and the physiology and genomics of this broad group of microorganisms are explored in the book. With contributions from an internationally recognized group of experts, the book integrates medicine, public health, environmental, and microbiological topics in order to provide a unique, holistic understanding of fecal bacteria. Moreover, it shows how the latest basic science and applied research findings are helping to solve problems and develop effective management strategies. For example, readers will discover how the latest tools and molecular approaches have led to our current understanding of fecal bacteria and enabled us to improve human health and water quality. The Fecal Bacteria is recommended for microbiologists, clinicians, animal scientists, engineers, environmental scientists, food safety experts, water quality managers, and students. It will help them better understand fecal bacteria and use their knowledge to protect human and environmental health. They can also apply many of the techniques and molecular tools discussed in this book to the study of a broad range of microorganisms in a variety of habitats.

Book↗