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At least 1,423 records · Page 79Linked to original sources

Conservation status of the American horseshoe crab, (Limulus polyphemus): A regional assessment

Horseshoe crabs have persisted for more than 200 million years, and fossil forms date to 450 million years ago. The American horseshoe crab ( Limulus polyphemus ), one of four extant horseshoe crab species, is found along the Atlantic coastline of North America ranging from Alabama to Maine, USA with another distinct population on the coasts of Campeche, Yucatán and Quintana Roo in the Yucatán Peninsula, México. Although the American horseshoe crab tolerates broad environmental conditions, exploitation and habitat loss threaten the species. We assessed the conservation status of the American horseshoe crab by comprehensively reviewing available scientific information on its range, life history, genetic structure, population trends and analyses, major threats, and conservation. We structured the status assessment by six genetically-informed regions and accounted for sub-regional differences in environmental conditions, threats, and management. The transnational regions are Gulf of Maine (USA), Mid-Atlantic (USA), Southeast (USA), Florida Atlantic (USA), Northeast Gulf of México (USA), and Yucatán Peninsula (México). Our conclusion is that the American horseshoe crab species is vulnerable to local extirpation and that the degree and extent of risk vary among and within the regions. The risk is elevated in the Gulf of Maine region due to limited and fragmented habitat. The populations of horseshoe crabs in the Mid-Atlantic region are stable in the Delaware Bay area, and regulatory controls are in place, but the risk is elevated in the New England area as evidenced by continuing declines understood to be caused by over-harvest. The populations of horseshoe crabs in the Southeast region are stable or increasing. The populations of horseshoe crabs in the Florida Atlantic region show mixed trends among areas, and continuing population reductions at the embayment level have poorly understood causes. Within the Northeast Gulf of Mexico, causes of population trends are poorly understood and currently there is no active management of horseshoe crabs. Horseshoe crabs within México have conservation protection based on limited and fragmented habitat and geographic isolation from other regions, but elevated risk applies to the horseshoe crabs in the Yucatán Peninsula region until sufficient data can confirm population stability. Future species status throughout its range will depend on the effectiveness of conservation to mitigate habitat loss and manage for sustainable harvest among and within regions.

Reviews in Fish Biology and Fisheries↗

Congruent population genetic structure but differing depths of divergence for three alpine stoneflies with similar ecology and geographic distributions

Comparative population genetic studies provide a powerful means for assessing the degree to which evolutionary histories may be congruent among taxa while also highlighting the potential for cryptic diversity within existing species. In the Rocky Mountains, three confamilial stoneflies ( Zapada glacier , Lednia tumana , and Lednia tetonica ; Plecoptera, Nemouridae) occupy cold alpine streams that are primarily fed by melting ice. Lednia tumana and L. tetonica are sister species diagnosed from systematic morphological differences, and they are endemic to areas surrounding Glacier National Park and Grand Teton National Park, respectively, in the U.S. Rocky Mountains. Zapada glacier is also present in alpine streams from Glacier National Park to the Teton Range, sometimes co‐occurring with either Lednia species. We used mitochondrial sequence data to clarify species boundaries, compare population genetic patterns, and test demographic models in a coalescent framework for the three stoneflies. We addressed four questions: (1) Is there genetic support for the morphology‐based species boundaries in Lednia ? (2) Is there genetic support for cryptic, or as‐yet undescribed, diversity within Z. glacier ? (3) Do similar geographic distributions and ecological requirements yield spatial congruence of genetic structure between high‐elevation Lednia and Z. glacier populations? (4) Is there evidence for contemporary gene flow among isolated populations in either group? Our results supported the existing taxonomy with Z. glacier and the two Lednia species differing in their depths of divergence among study regions (e.g. maximum sequence divergence within Z. glacier = 1.2% versus 5% between L. tumana and L. tetonica ). However, spatial population genetic patterns were broadly congruent, indicating stonefly populations isolated on mountaintop islands. Coalescent modelling supported the possibility of rare, extremely limited contemporary gene flow among Z. glacier populations, with no support for gene flow between L. tumana and L. tetonica . The focal stoneflies and associated assemblages occupy the highest elevation, coldest permanent alpine streams in the study region. This lotic habitat type faces an uncertain future under a diminishing alpine cryosphere. Given spatial congruence of genetic structure demonstrating unique biodiversity associated with individual alpine islands, we encourage conservation management strategies be developed and applied at corresponding spatial scales.

Montana, Wyoming↗

Soil characteristics are associated with gradients of big sagebrush canopy structure after disturbance

Reestablishing shrub canopy cover after disturbance in semi-arid ecosystems, such as sagebrush steppe, is essential to provide wildlife habitat and restore ecosystem functioning. While several studies have explored the effects of landscape and climate factors on the success or failure of sagebrush seeding, the influence of soil properties on gradients of shrub canopy structure in successfully seeded areas remains largely unexplored. In this study, we evaluated associations between soil properties and gradients in sagebrush canopy structure in stands that had successfully reestablished after fire and subsequent seeding treatments. Using a dataset collected across the Great Basin, USA, of sagebrush stands that had burned and reestablished between 1986 and 2013, we tested soil depth and texture, soil surface classification, biological soil crusts plus mean historical precipitation, solar heatload, and fire history as modeling variables to explore gradients in sagebrush canopy structure growth in terms of cover, height, and density. Deeper soils were associated with greater sagebrush canopy structure development in terms of plant density and percent cover, coarser textured soils were associated with greater sagebrush cover and density, and more clayey soils were typically associated with greater height. Biological crust presence was also positively associated with enhanced sagebrush canopy growth, but adding more demographically or morphologically explicit descriptions of biocrust communities did not improve explanatory power. Increasing heatload had a negative effect on sagebrush canopy structure growth, and increased mean annual precipitation was only associated with greater sagebrush height. Given that conservation and restoration of the sagebrush steppe ecosystems has become a priority for land managers, the associations we identify between gradients in post-fire sagebrush canopy structure growth and field-identifiable soil characteristics may improve planning of land treatments for sagebrush restoration and the understanding of semi-arid ecosystem functioning and post-disturbance dynamics.

Ecosphere↗

Increased scientific rigor will improve reliability of research and effectiveness of management

Rigorous science that produces reliable knowledge is critical to wildlife management because it increases accurate understanding of the natural world and informs management decisions effectively. Application of a rigorous scientific method based on hypothesis testing minimizes unreliable knowledge produced by research. To evaluate the prevalence of scientific rigor in wildlife research, we examined 24 issues of the Journal of Wildlife Management from August 2013 through July 2016. We found 43.9% of studies did not state or imply a priori hypotheses, which are necessary to produce reliable knowledge. We posit that this is due, at least in part, to a lack of common understanding of what rigorous science entails, how it produces more reliable knowledge than other forms of interpreting observations, and how research should be designed to maximize inferential strength and usefulness of application. Current primary literature does not provide succinct explanations of the logic behind a rigorous scientific method or readily applicable guidance for employing it, particularly in wildlife biology; we therefore synthesized an overview of the history, philosophy, and logic that define scientific rigor for biological studies. A rigorous scientific method includes 1) generating a research question from theory and prior observations, 2) developing hypotheses (i.e., plausible biological answers to the question), 3) formulating predictions (i.e., facts that must be true if the hypothesis is true), 4) designing and implementing research to collect data potentially consistent with predictions, 5) evaluating whether predictions are consistent with collected data, and 6) drawing inferences based on the evaluation. Explicitly testing a priori hypotheses reduces overall uncertainty by reducing the number of plausible biological explanations to only those that are logically well supported. Such research also draws inferences that are robust to idiosyncratic observations and unavoidable human biases. Offering only post hoc interpretations of statistical patterns (i.e., a posteriori hypotheses) adds to uncertainty because it increases the number of plausible biological explanations without determining which have the greatest support. Further, post hoc interpretations are strongly subject to human biases. Testing hypotheses maximizes the credibility of research findings, makes the strongest contributions to theory and management, and improves reproducibility of research. Management decisions based on rigorous research are most likely to result in effective conservation of wildlife resources.

Journal of Wildlife Management↗

Using microbial source tracking to identify fecal contamination sources in an embayment in Hempstead Harbor on Long Island, New York

The U.S. Geological Survey worked collaboratively with the New York State Department of Environmental Conservation to assess the potential sources of fecal contamination entering Hempstead Harbor, an embayment on the northern shore of Nassau County, Long Island, New York. Water samples are routinely collected by the New York State Department of Environmental Conservation in the harbor and analyzed for fecal coliform bacteria, an indicator of fecal contamination, to determine the need for closure of shellfish beds for harvest and consumption. Fecal coliform and other bacteria are an indicator of the potential presence of pathogenic (disease-causing) bacteria. However, indicator bacteria alone cannot determine the biological or geographical sources of contamination; therefore, microbial source tracking was implemented to determine various biological sources of contamination. In addition, information such as the location, weather and season, surrounding land use, and additional water-quality data (including nutrient and stable isotopes of nitrate analyses) for the location where a sample was collected help determine the geographical source and conveyance of land-based water to the embayment. Our analysis revealed an abundance of human and canine fecal contamination throughout the Hempstead Harbor landscape and that water from municipal separate storm sewer system conveyances was the most likely transport mechanism of this fecal contamination. Resuspension of bed sediment may contribute to fecal contamination in the harbor, but more targeted analyses are needed to support this finding. There was little evidence of groundwater-contributing fecal bacteria by direct discharge from the subsurface. A classification scheme was developed to convey the degree of fecal contamination to stakeholders and resource managers. Based on this classification scheme, the culvert at Glenwood Road and the outfall and the spillway at Skillman Street were identified as locations that contribute substantial fecal contamination to Hempstead Harbor.

New York↗

Using microbial source tracking to identify contamination sources in Port Jefferson Harbor, Setauket Harbor, and Conscience Bay on Long Island, New York

The U.S. Geological Survey worked in cooperation with the New York State Department of Environmental Conservation to assess the potential sources of fecal contamination entering Port Jefferson Harbor, Setauket Harbor, and Conscience Bay, an embayment complex on the northern shore of Suffolk County, Long Island, New York. Water samples are routinely collected by the New York State Department of Environmental Conservation in the harbor and analyzed for fecal coliform bacteria, an indicator of fecal contamination, to determine the need for closure of shellfish beds for harvest and consumption. Fecal coliform and other bacteria are an indicator of the potential presence of pathogenic (disease-causing) bacteria. However, indicator bacteria alone cannot determine the biological or geographical sources of contamination; therefore, microbial source tracking was implemented to determine various biological sources of contamination. In addition, information such as the location, weather and season, and surrounding land use where a sample was collected help determine the geographical source and conveyance of land-based water to the embayment. Our analysis revealed that the most substantial source of fecal contamination to the Port Jefferson Harbor complex was discharge from sites draining ponds and wetlands, particularly during the summer months. Fecal coliform bacteria at sites where ponds and wetlands drain are increased by stormwater runoff, which is another substantial source of fecal contamination. Human markers, likely originating from the outfall of the Port Jefferson sewage treatment plant, were found at least once in every sample collected within Port Jefferson Harbor; however, fecal coliform concentrations were low, indicating that the sewage treatment plant is not a likely source of fecal contamination to the embayment. Canine markers detected in Conscience Bay were associated with high fecal coliform, particularly in wet summer samples. Waterfowl markers were most often detected in source water for Port Jefferson Harbor, but in Conscience Bay, waterfowl was frequently detected within the bay itself. Resuspension of bed sediment may contribute to fecal contamination in the harbor, but more targeted analyses are needed to support this finding. There was little evidence of groundwater-contributing fecal bacteria by direct discharge from the subsurface. A classification scheme was developed to convey the degree of fecal contamination to stakeholders and resource managers. Based on this classification scheme, the Culvert North of State Route 25A, Culvert North of Shore Road, Old Mill Creek Culvert, and Mill Pond Culvert to Conscience Bay sites were identified as locations that contribute substantial fecal contamination to the Port Jefferson Harbor complex.

New York↗

Using microbial source tracking to identify fecal contamination sources in Patchogue and Bellport Bays on Long Island, New York

The U.S. Geological Survey worked in cooperation with the New York State Department of Environmental Conservation to assess the potential sources of fecal contamination entering Patchogue and Bellport Bays, two embayments on the south shore of Suffolk County, Long Island, New York. Water samples are routinely collected by the New York State Department of Environmental Conservation in the bays and analyzed for fecal coliform bacteria, an indicator of fecal contamination, to determine the need for closure of shellfish beds for harvest and consumption. Fecal coliform and other bacteria are an indicator of the potential presence of pathogenic (disease-causing) bacteria. However, indicator bacteria alone cannot determine the biological or geographical sources of contamination; therefore, microbial source tracking was implemented to determine various biological sources of contamination. In addition, information such as the location, weather and season, and surrounding land use where a sample was collected help determine the geographical source and conveyance of land-based water to the embayment. Analysis revealed that the most substantial source of fecal contamination to Patchogue and Bellport Bays was discharge from sites draining ponds and wetlands into the rivers and tributaries sampled, particularly during the summer months. Fecal coliform bacteria at sites where ponds and wetlands drain are increased by stormwater runoff, which is another substantial source of fecal contamination. Overall, canine- and waterfowl-associated bacterial contributions were prevalent in source samples in both bays. Human-associated markers were present in surface-water source samples and completely absent in receptor samples in Patchogue Bay. The Fireplace Neck receptor site in Bellport Bay had a human-associated marker present in the summer wet sample only. Human markers were detected at the sample site downstream from the Patchogue wastewater treatment plant but were associated with low fecal coliform concentrations, indicating that the wastewater treatment plant is not a likely source of fecal contamination to Patchogue Bay. The lack of human-associated marker detections within Patchogue and Bellport Bays in summer source samples coupled with low to no detections of fecal coliform, especially where small marinas are present in creeks and tributaries, suggest that boats do not substantially contribute fecal coliform bacteria to the bays. There was little evidence of groundwater-contributing fecal bacteria by direct discharge from the subsurface. Further, the sandy sediment alongside Patchogue and Bellport Bays is unlikely to contribute fecal coliform bacteria from the test host organisms when resuspended in the water column. A classification scheme was developed to convey the degree of fecal contamination to stakeholders and resource managers. Based on this classification scheme, the Corey Creek Near Middle Road, Patchogue River Near Division Street, and Swan River Mouth sampling sites were identified as locations that contribute substantial fecal contamination to Patchogue Bay. In Bellport Bay, the Culvert at Beaverdam Creek site was identified as the location contributing the most substantial fecal contamination.

New York↗

Using microbial source tracking to identify fecal contamination sources in Sag Harbor on Long Island, New York

The U.S. Geological Survey worked in cooperation with the New York State Department of Environmental Conservation to assess the potential sources of fecal contamination entering Sag Harbor, an embayment complex on the northern shore of the south fork of Suffolk County, Long Island, New York. Water samples are routinely collected by the New York State Department of Environmental Conservation in the harbor and analyzed for fecal coliform bacteria, an indicator of fecal contamination, to determine the need for closure of shellfish beds for harvest and consumption. Fecal coliform and other bacteria are an indicator of the potential presence of pathogenic (disease-causing) bacteria. However, indicator bacteria alone cannot determine the biological or geographical sources of contamination; therefore, microbial source tracking was implemented to determine various biological sources of contamination. In addition, information such as the location, weather and season, and surrounding land use where a sample was collected help determine the geographical source and conveyance of land-based water to the embayment. Analysis revealed that the most substantial source of fecal contamination to Sag Harbor was discharge from sites draining ponds and wetlands, particularly during the summer months. Fecal coliform bacteria at sites where ponds and wetlands drain are increased by stormwater runoff, which is another substantial source of fecal contamination. Human markers were detected in all four samples at the Sag Harbor Sewage Treatment Plant Outfall site but were associated with low fecal coliform concentrations, indicating that the sewage treatment plant is not a likely source of fecal contamination to the embayment. The Ligonee Brook Culvert, Paynes Creek near Marjorie Lane, and Otter Pond Culvert sites were identified as locations that contribute fecal contamination to Sag Harbor. These three locations had high fecal coliform bacteria concentrations in the summer, one of which was positive for canine microbial source tracking markers (Ligonee Brook Culvert), and another positive for waterfowl markers (Paynes Creek near Marjorie Lane). The absence of fecal coliform bacteria and human microbial source tracking markers in groundwater samples indicates that water from septic systems does not influence the harbor; however, elevated fecal coliform bacteria concentrations were not often detected. Further, the sandy sediment alongside Sag Harbor is unlikely to contribute fecal coliform bacteria when resuspended in the water column through tidal shifts or boat activity.

New York↗

Using microbial source tracking to identify fecal contamination sources in Lake Montauk on Long Island, New York

The U.S. Geological Survey worked in cooperation with the Concerned Citizens of Montauk and the New York State Department of Environmental Conservation to assess the potential sources of fecal contamination entering Lake Montauk, an artificial embayment on the tip of the southern fork of Suffolk County, Long Island, New York. Water samples are routinely collected by the New York State Department of Environmental Conservation in the harbor and analyzed for fecal coliform bacteria, an indicator of fecal contamination, to determine the need for closure of shellfish beds for harvest and consumption. Fecal coliform and other bacteria are an indicator of the potential presence of pathogenic (disease-causing) bacteria. However, indicator bacteria alone cannot determine the biological or geographical sources of contamination; therefore, microbial source tracking was implemented to determine various biological sources of contamination. In addition, information such as the location, weather and season, and surrounding land use where a sample was collected help determine the geographical source and conveyance of land-based water to the embayment. Overall, human and waterfowl markers were infrequently and sporadically present in source and receptor samples at low concentrations. By evaluating the microbial source tracking markers alongside fecal coliform data and land-use information, geographical sources of fecal contamination discharging from various source sites, such as culverts and ponds, were better differentiated. Analysis revealed that stormwater runoff and pond drainage were the most likely transport mechanisms for fecal contamination to Lake Montauk. When considering Lake Montauk as a whole, the highest frequency of fecal coliform detections in source site samples was found to be under wet summer conditions, as evidenced by the high fecal coliform concentrations at the South Beach, Stepping Stones Pond, and Stepping Stones Pond Culvert sites (300, 220, and more than 16,000 most probable number per 100 milliliters, respectively). No point sources of fecal coliform contamination to Lake Montauk were identified; however, receptor site samples adjacent to marinas (Lake Montauk Inlet and Star Island North sites) had a high frequency of human marker detections but were associated with fecal coliform concentrations at or below the reporting limit. The absence of fecal coliform and human microbial source tracking markers in groundwater samples indicated that water from septic systems did not influence the lake during this study. Further, the sandy sediment sample collected at the South Beach site was negative for all microbial source tracking markers and is unlikely to contribute fecal coliform from the tested host organisms when resuspended in the water column through tidal shifts or boat activity.

New York↗

Using microbial source tracking to identify fecal contamination sources in Great South Bay on Long Island, New York

The U.S. Geological Survey worked in cooperation with the New York State Department of Environmental Conservation to assess the potential sources of fecal contamination entering a part of Great South Bay (referred to as Great South Bay for the purposes of this report) near the hamlets of West Sayville, Sayville, and Bayport on the southern shore of Suffolk County on Long Island, New York. Water samples are routinely collected by the New York State Department of Environmental Conservation in the bay and analyzed for fecal coliform bacteria, an indicator of fecal contamination, to determine the need for closure of shellfish beds for harvest and consumption. Fecal coliform and other bacteria are an indicator of the potential presence of pathogenic (disease-causing) bacteria. However, indicator bacteria alone cannot determine the biological or geographical sources of contamination; therefore, microbial source tracking was implemented to determine various biological sources of contamination. In addition, information such as the location, weather and season, and surrounding land use where a sample was collected help determine the geographical source and conveyance of land-based water to the embayment. Analysis revealed that the most substantial source of fecal contamination to Great South Bay was discharge from sites draining ponds and wetlands into the tributaries sampled, Brown and Green Creeks, particularly during the summer months. Fecal coliform bacteria at sites where ponds and wetlands drain are increased by stormwater runoff, which is another substantial source of fecal contamination. Sites with high concentrations of fecal coliform bacteria in the summer exacerbated by stormwater include the Brown Creek Culvert at Middle Road, Mill Pond Culvert near South Street, and Green Creek Culvert near Montauk Highway sites. The canine Bacteroides (BacCan) marker was the most frequently detected microbial source tracking marker with 15 positive detections in surface water across the landscape, followed by the waterfowl Helicobacter (GFD) marker with 9 detections and the human Bacteroides (HF183) marker with 4 detections in surface water (excluding 2 detections in sediment). The ruminant Bacteroides (Rum2Bac) marker was not detected in any samples collected during this study. The detection frequency of BacCan was similar for all sampling conditions and seasons, suggesting canine influence is unrelated to weather events and is a year-round occurrence. BacCan was detected in 14 of 16 source samples and only 1 of 16 receptor samples, which suggests that canine fecal contamination is likely diluted in the bay. There was a similar amount of marker detections when comparing weather condition (wet or dry) and season (winter or summer), suggesting that fecal contamination was unrelated to weather events or time of year. Eight waterfowl marker detections were in samples collected during the winter, and only one during the summer, implicating seasonal avian fecal contamination throughout the embayment. The human marker was detected in only one surface-water receptor sample, during the wet winter sampling event at the Green Creek Mid-Bay site. Three of four human marker detections were in the samples collected during the wet winter sampling event, indicating that weather and season may influence the presence of human markers in Great South Bay, but human markers are not overly prevalent.

New York↗

Using microbial source tracking to identify fecal contamination sources in South Oyster Bay on Long Island, New York

The U.S. Geological Survey worked in cooperation with the New York State Department of Environmental Conservation to assess the potential sources of fecal contamination entering South Oyster Bay, a shallow embayment on the southern shore of Long Island, New York. Water samples are routinely collected by the New York State Department of Environmental Conservation in the bay and analyzed for fecal coliform bacteria, an indicator of fecal contamination, to determine the need for closure of shellfish beds for harvest and consumption. Fecal coliform and other bacteria are an indicator of the potential presence of pathogenic (disease-causing) bacteria. However, indicator bacteria alone cannot determine the biological or geographical sources of contamination; therefore, microbial source tracking was implemented to determine various biological sources of contamination. In addition, information such as the location, weather and season, and surrounding land use where a sample was collected help determine the geographical source and conveyance of land-based water to the embayment. Analysis revealed that the most substantial source of fecal contamination to South Oyster Bay was stormwater, particularly during the summer months. The highest frequency of fecal coliform detections in source sites were under wet summer conditions, and the highest fecal coliform concentrations were under wet summer conditions at the Cedar Creek near Bay Place and Unqua Lake Culvert sites (more than 16,000 most probable number per 100 milliliters each). The human-associated Bacteroides marker was the most frequently detected microbial source tracking marker in South Oyster Bay (50 percent positive detections). The human marker was detected at least twice in all surface water source and receptor sites, except for the Massapequa Lake East Culvert source site that did not have any positive human marker detections. Canine contamination was prolific at source sites but was associated with low fecal coliform concentrations in the winter months. All detections of the canine-associated Bacteroides marker were in samples collected during the winter season and were associated with fecal coliform concentrations below the reporting limit, indicating that birds are not a persistent source of fecal coliform to South Oyster Bay. The absence of fecal coliform and human markers in groundwater samples collected throughout the larger study area indicates that water from cesspools or septic tanks do not contribute fecal coliform to the bay. Further, microbial source tracking markers were not detected in the sandy sediment collected at Zachs Bay. Based a classification scheme developed to convey the degree of fecal contamination to stakeholders and resource managers, the Cedar Creek near Bay Place and Unqua Lake Culvert sites were identified as locations that contribute substantial fecal contamination to South Oyster Bay.

New York↗

Drivers of landscape change in the northwest boreal region

The northwest boreal region (NWB) of North America is a land of extremes. Extending more than 1.3 million square kilometers (330 million acres), it encompasses the entire spectrum between inundated wetlands below sea level to the tallest peak in North America. Permafrost gradients span from nearly continuous to absent. Boreal ecosystems are inherently dynamic and continually change over decades to millennia. The braided rivers that shape the valleys and wetlands continually change course, creating and removing vast wetlands and peatlands. Glacial melt, erosion, fires, permafrost dynamics, and wind-blown loess are among the shaping forces of the landscape. As a result, species interactions and ecosystem processes are shifting across time. The NWB is a data-poor region, and the intention of the NWB Landscape Conservation Cooperative is to determine what data are not available and what data are available. For instance, historical baseline data describing the economic and social relationships in association with the ecological condition of the NWB landscape are often lacking. Likewise, the size and remoteness of this region make it challenging to measure basic biological information, such as species population sizes or trends. The paucity of weather and climate monitoring stations also compound the ability to model future climate trends and impacts, which is part of the nature of working in the north. The purpose of this volume is to create a resource for regional land and resource managers and researchers by synthesizing the latest research on the historical and current status of landscape-scale drivers (including anthropogenic activities) and ecosystem processes, future projected changes of each, and the effects of changes on important resources. Generally, each chapter is coauthored by researchers and land and natural resource managers from the United States and Canada.

Northwest Boreal Region↗

Directional connectivity in hydrology and ecology

Quantifying hydrologic and ecological connectivity has contributed to understanding transport and dispersal processes and assessing ecosystem degradation or restoration potential. However, there has been little synthesis across disciplines. The growing field of ecohydrology and recent recognition that loss of hydrologic connectivity is leading to a global decline in biodiversity underscore the need for a unified connectivity concept. One outstanding need is a way to quantify directional connectivity that is consistent, robust to variations in sampling, and transferable across scales or environmental settings. Understanding connectivity in a particular direction (e.g., streamwise, along or across gradient, between sources and sinks, along cardinal directions) provides critical information for predicting contaminant transport, planning conservation corridor design, and understanding how landscapes or hydroscapes respond to directional forces like wind or water flow. Here we synthesize progress on quantifying connectivity and develop a new strategy for evaluating directional connectivity that benefits from use of graph theory in ecology and percolation theory in hydrology. The directional connectivity index (DCI) is a graph-theory based, multiscale metric that is generalizable to a range of different structural and functional connectivity applications. It exhibits minimal sensitivity to image rotation or resolution within a given range and responds intuitively to progressive, unidirectional change. Further, it is linearly related to the integral connectivity scale length—a metric common in hydrology that correlates well with actual fluxes—but is less computationally challenging and more readily comparable across different landscapes. Connectivity-orientation curves (i.e., directional connectivity computed over a range of headings) provide a quantitative, information-dense representation of environmental structure that can be used for comparison or detection of subtle differences in the physical-biological feedbacks driving pattern formation. Case-study application of the DCI to the Everglades in south Florida revealed that loss of directional hydrologic connectivity occurs more rapidly and is a more sensitive indicator of declining ecosystem function than other metrics (e.g., habitat area) used previously. Here and elsewhere, directional connectivity can provide insight into landscape drivers and processes, act as an early-warning indicator of environmental degradation, and serve as a planning tool or performance measure for conservation and restoration efforts.

Ecological Applications↗

Review of trap-and-haul for managing Pacific salmonids (Oncorhynchus spp.) in impounded river systems

High-head dams are migration barriers for Pacific salmon Oncorhynchus spp. in many river systems and recovery measures for impacted stocks are limited. Trap-and-haul has been widely used in attempts to facilitate recovery but information from existing programs has not been synthesized to inform improvements to aid recovery of salmonids in systems with high-head dams. We reviewed 17 trap-and-haul programs regarding Pacific salmon to: (1) summarize information about facility design, operation and biological effects; (2) identify critical knowledge gaps; and (3) evaluate trap-and-haul as a current and future management tool. Existing programs are operated to address a range of management goals including restoring access to historical habitats, temporarily reducing exposure to dangerous in-river conditions, and reintroducing ecological processes upstream from dams. Information gathered from decades of operation on facility design criteria and fish handling protocols, and robust literature on fish collection and passage are available. While many aspects of trap-and-haul have been evaluated, effects on population productivity and sustainability remain poorly understood. Long-term and systematic studies of trap-and-haul outcomes are rare, and assessments can be confounded by concurrent management actions and broad ecological and climatic effects. Existing data suggest that performance and effectiveness vary among programs and over various time scales within programs. Although critical information gaps exist, trap-and-haul is an important management and conservation tool for providing Pacific salmonids access to historical habitats. Successful application of trap-and-haul programs requires long-term commitment and an adaptive management approach by dam owners and stakeholders, and careful planning of new programs.

California, Idaho, Oregon, Washington↗

Spatially explicit decision support for selecting translocation areas for Mojave desert tortoises

Spatially explicit decision support systems are assuming an increasing role in natural resource and conservation management. In order for these systems to be successful, however, they must address real-world management problems with input from both the scientific and management communities. The National Training Center at Fort Irwin, California, has expanded its training area, encroaching U.S. Fish and Wildlife Service critical habitat set aside for the Mojave desert tortoise ( Gopherus agassizii ), a federally threatened species. Of all the mitigation measures proposed to offset expansion, the most challenging to implement was the selection of areas most feasible for tortoise translocation. We developed an objective, open, scientifically defensible spatially explicit decision support system to evaluate translocation potential within the Western Mojave Recovery Unit for tortoise populations under imminent threat from military expansion. Using up to a total of 10 biological, anthropogenic, and/or logistical criteria, seven alternative translocation scenarios were developed. The final translocation model was a consensus model between the seven scenarios. Within the final model, six potential translocation areas were identified.

Biodiversity and Conservation↗

Implications of estuarine transport for water quality

In this chapter, some implications of estuarine transport for water quality are discussed. This is not an exhaustive review of all physical processes potentially important to water quality in estuaries. Rather, the focus is on (1) some fundamental relationships, concepts, and helpful idealizations (e.g., evolution equations for reactive scalars, transport time scales, scaling and non-dimensional numbers), (2) some common and often dominant physical processes in terms of their influence on estuarine water quality (e.g., stratification and turbulent mixing), and (3) some less prevalently discussed but probably widely important issues regarding high-frequency (i.e., intradaily) processes and their influence on water quality. Here, “water quality” refers to the full range of suspended constituents (or “scalars”, i.e., non-vector quantities) in an estuarine water column. These constituents may be dissolved or particulate, mineral, chemical, or biological, or they may represent physical properties of the water (e.g., temperature). The spatial distribution of a water quality constituent is influenced by the hydrodynamic environment in which it is suspended, but it may be additionally subject to motility, positive buoyancy, or negative buoyancy (e.g., some phytoplankton or zooplankton). Water quality scalars may be conservative (i.e., non-reactive, such as salt) or non-conservative (i.e., reactive and thereby potentially changing in concentration or form during transit; e.g., nitrogen, phosphorus, or phytoplankton). Hydrodynamic and transport processes are important not only because they “move stuff around” but also because, in the case of reactive scalars, those processes may expose the scalars to a range of environments, each of which may be associated with distinct rates of scalar transformation.

Book chapter↗

Assessing biological effects from highway-runoff constituents

Increased emphasis on evaluation of nonpoint-source pollution has intensified the need for techniques that can be used to discern the toxicological effects of complex chemical mixtures. In response, the use of biological assessment techniques is receiving increased regulatory emphasis. When applied with documented habitat assessment and chemical analysis, these techniques can increase our understanding of the influence of environmental contaminants on the biological integrity and ecological function of aquatic communities. The contaminants of greatest potential concern in highway runoff are those that arise from highway construction, maintenance, and use. The major contaminants of interest are deicers; nutrients; metals; petroleum-related organic compounds, such as polycyclic aromatic hydrocarbons (PAHs), benzene, toluene, ethylbenzene, and xylene (BTEX), and methyl tert -butyl ether (MTBE); sediment washed off the road surface; and agricultural chemicals used in highway maintenance. Hundreds, if not thousands, of biological endpoints (measurable responses of living organisms) may be either directly or associatively affected by contaminant exposure. Measurable effects can occur throughout ecosystem processes across the wide range of biological complexity, ranging from responses at the biochemical level to the community level. The challenge to the environmental scientist is to develop an understanding of the relationship of effects at various levels of biological organization in order to determine whether a causal relationship exists between chemical exposure and substantial ecological impairment. This report provides a brief history of the evolution of biological assessment techniques, a description of the major classes of contaminants that are of particular interest in highway runoff, an overview of representative biological assessment techniques, and a discussion of data-quality considerations. Published reports with a focus on the effects of highway runoff on the local ecosystem were reviewed to provide information on (1) the suitability of the existing data for a quantitative national synthesis, (2) the methods available to study the effects of highway runoff on local ecosystems, and (3) the potential for adverse effects on the roadside environment and receiving waters. Although many biological studies have been done, the use of different methods and a general lack of sufficient documentation precludes a quantitative national synthesis on the basis of the existing data. The Federal Highway Administration, the U.S. Environmental Protection Agency, the U.S. Geological Survey, the Intergovernmental Task Force on Monitoring Water Quality, and the National Resources Conservation Service all have developed and documented methods for assessing the effects of contaminants on ecosystems in receiving waters. These published methods can be used to formulate a set of protocols to provide consistent information from highway-runoff studies. Review of the literature indicates (qualitatively) that highway runoff (even from highways with high traffic volume) may not usually be acutely toxic. Tissue analysis and community assessments, however, indicate effects from highway- runoff sediments near discharge points (even from sites near highways with relatively low traffic volumes). At many sites, elevated concentrations of highway-runoff constituents were measured in tissues of species associated with aquatic sediments. Community assessments also indicate decreases in the diversity and productivity of aquatic ecosystems at some sites receiving highway runoff. These results are not definitive, however, and depend on many site-specific criteria that were not sufficiently documented in most of the studies reviewed.

Open-File Report↗

Applications of spatial statistical network models to stream data

Streams and rivers host a significant portion of Earth's biodiversity and provide important ecosystem services for human populations. Accurate information regarding the status and trends of stream resources is vital for their effective conservation and management. Most statistical techniques applied to data measured on stream networks were developed for terrestrial applications and are not optimized for streams. A new class of spatial statistical model, based on valid covariance structures for stream networks, can be used with many common types of stream data (e.g., water quality attributes, habitat conditions, biological surveys) through application of appropriate distributions (e.g., Gaussian, binomial, Poisson). The spatial statistical network models account for spatial autocorrelation (i.e., nonindependence) among measurements, which allows their application to databases with clustered measurement locations. Large amounts of stream data exist in many areas where spatial statistical analyses could be used to develop novel insights, improve predictions at unsampled sites, and aid in the design of efficient monitoring strategies at relatively low cost. We review the topic of spatial autocorrelation and its effects on statistical inference, demonstrate the use of spatial statistics with stream datasets relevant to common research and management questions, and discuss additional applications and development potential for spatial statistics on stream networks. Free software for implementing the spatial statistical network models has been developed that enables custom applications with many stream databases.

WIREs Water↗