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A culture-based survey of fungi in soil from bat hibernacula in the eastern United States and its implications for detection of Geomyces destructans, the causal agent of bat white-nose syndrome

The recent emergence of white-nose syndrome (WNS), a fungal disease causing unprecedented mortality among hibernating bats of eastern North America, has revealed a knowledge gap regarding fungal communities associated with bats and their hibernacula. We used culture-based techniques to investigate the diversity of fungi in soil samples collected from 24 bat hibernacula in the eastern United States. Ribosomal RNA regions (internal transcribed spacer and partial intergenic spacer) were sequenced to preliminarily characterize isolates. Geomyces species were one of the most abundant and diverse groups cultured, representing approximately 33% of all isolates. Geomyces destructans was isolated from soil samples from three hibernacula in states where WNS is known to occur, and many of the other cultured Geomyces isolates likely represent undescribed taxa. Further characterization of the diversity of fungi that occur in hibernacula will both facilitate an improved understanding of the ecology of G. destructans within this complex fungal community and provide an opportunity to identify characteristics that differentiate G. destructans from non-pathogenic relatives.

Mycologia↗

Differences in breeding bird assemblages related to reed canary grass cover cover and forest structure on the Upper Mississippi River

Floodplain forest of the Upper Mississippi River provides habitat for an abundant and diverse breeding bird community. However, reed canary grass Phalaris arundinacea invasion is a serious threat to the future condition of this forest. Reed canary grass is a well-known aggressive invader of wetland systems in the northern tier states of the conterminous United States. Aided by altered flow regimes and nutrient inputs from agriculture, reed canary grass has formed dense stands in canopy gaps and forest edges, retarding tree regeneration. We sampled vegetation and breeding birds in Upper Mississippi River floodplain forest edge and interior areas to 1) measure reed canary grass cover and 2) evaluate whether the breeding bird assemblage responded to differences in reed canary grass cover. Reed canary grass was found far into forest interiors, and its cover was similar between interior and edge sites. Bird assemblages differed between areas with more or less reed canary grass cover (.53% cover breakpoint). Common yellowthroat Geothlypis trichas , black-capped chickadee Parus atricapillus , and rose-breasted grosbeak Pheucticus ludovicianus were more common and American redstart Setophaga ruticilla , great crested flycatcher Myiarchus crinitus , and Baltimore oriole Icterus galbula were less common in sites with more reed canary grass cover. Bird diversity and abundance were similar between sites with different reed canary grass cover. A stronger divergence in bird assemblages was associated with ground cover ,15%, resulting from prolonged spring flooding. These sites hosted more prothonotary warbler Protonotaria citrea , but they had reduced bird abundance and diversity compared to other sites. Our results indicate that frequently flooded sites may be important for prothonotary warblers and that bird assemblages shift in response to reed canary grass invasion.

Upper Mississippi River↗

Fire effects on wildlife in Central Hardwoods and Appalachian regions

Fire is being prescribed and used increasingly to promote ecosystem restoration (e.g., oak woodlands and savannas) and to manage wildlife habitat in the Central Hardwoods and Appalachian regions, USA. However, questions persist as to how fire affects hardwood forest communities and associated wildlife, and how fire should be used to achieve management goals. We provide an up-to-date review of fire effects on various wildlife species and their habitat in the Central Hardwoods and Appalachians. Documented direct effects (i.e., mortality) on wildlife are rare. Indirect effects (i.e., changes in habitat quality) are influenced greatly by light availability, fire frequency, and fire intensity. Unless fire intensity is great enough to kill a portion of the overstory, burning in closed-canopy forests has provided little benefit for most wildlife species in the region because it doesn’t result in enough sunlight penetration to elicit understory response. Canopy reduction through silvicultural treatment has enabled managers to use fire more effectively. Fire intensity must be kept low in hardwoods to limit damage to many species of overstory trees. However, wounding or killing trees with fire benefits many wildlife species by allowing increased sunlight to stimulate understory response, snag and subsequent cavity creation, and additions of large coarse woody debris. In general, a fire-return interval of 2 yr to 7 yr benefits a wide variety of wildlife species by providing a diverse structure in the understory; increasing browse, forage, and soft mast; and creating snags and cavities. Historically, dormant-season fire was most prevalent in these regions, and it still is when most prescribed fire is implemented in hardwood systems as burn-days are relatively few in the growing season of May through August because of shading from leaf cover and high fuel moisture. Late growing-season burning increases the window for burning, and better control on woody composition is possible. Early growing-season fire may pose increased risk for some species, especially herpetofauna recently emerged from winter hibernacula (April) or forest songbirds that nest in the understory (May to June). However, negative population-level effects are unlikely unless the burned area is relatively large and early growing-season fire is used continually. We did not find evidence that fire is leading to population declines for any species, including Endangered Species Act (ESA)-listed species (e.g., Indiana bat [ Myotis sodalis Mill. Allen] or northern long-eared bat [ M. septentrionalis Trouess.]). Instead, data indicate that fire can enhance habitat for bats by increasing suitability of foraging and day-roost sites. Similarly, concern over burning and displacement of woodland salamanders (Plethodontidae), another taxa of heightened conservation concern, is alleviated when fire is prescribed along ecologically appropriate aspect and slope gradients and not forced into mesic, high site index environments where salamanders are most common. Because topography across the Central Hardwoods and Appalachians is diverse, we contend that applying fire on positions best suited for burning is an effective approach to increase regional landscape heterogeneity and biological diversity. Herein, we offer prescriptive concepts for burning for various wildlife species and guilds in the Central Hardwoods and Appalachians.

Appalachian region, Central Hardwoods region↗

Impacts of mastication fuel treatments on California, USA, chaparral vegetation structure and composition

Mechanical fuel treatments are a primary pre-fire strategy for potentially mitigating the threat of wildland fire, yet there is limited information on how they impact shrubland ecosystems. Our goal was to assess the impact of mechanical mastication fuel treatments on chaparral vegetation and to determine the extent to which they emulate early post-fire succession. Mastication treatments significantly reduced the height and cover of woody vegetation and increased herbaceous cover and diversity. Non-native cover, density, and diversity were also significantly higher in masticated treatments. Comparisons with post-fire data from two studies showed that certain ephemeral post-fire endemics were absent or of limited occurrence from masticated plots in comparison to their abundance on adjacent burned plots. Structurally, masticated sites differed in the dense woody debris cover, whereas burned sites had little such ground cover. Regional comparison of masticated plots to previously published post-fire studies found that burned sites had greater cover, density, and diversity of native species. However, masticated sites and burned sites were broadly similar in distribution of different growth forms. Results from our study show that the use of mastication fuel treatments in chaparral are not in alignment with some resource conservation goals, but in some cases it is recognized that such sacrifice of natural resources may be an acceptable tradeoff to potentially mitigating fire hazard.

California↗

Collaborative decision-analytic framework to maximize resilience of tidal marshes to climate change

Decision makers that are responsible for stewardship of natural resources face many challenges, which are complicated by uncertainty about impacts from climate change, expanding human development, and intensifying land uses. A systematic process for evaluating the social and ecological risks, trade-offs, and cobenefits associated with future changes is critical to maximize resilience and conserve ecosystem services. This is particularly true in coastal areas where human populations and landscape conversion are increasing, and where intensifying storms and sea-level rise pose unprecedented threats to coastal ecosystems. We applied collaborative decision analysis with a diverse team of stakeholders who preserve, manage, or restore tidal marshes across the San Francisco Bay estuary, California, USA, as a case study. Specifically, we followed a structured decision-making approach, and we using expert judgment developed alternative management strategies to increase the capacity and adaptability to manage tidal marsh resilience while considering uncertainties through 2050. Because sea-level rise projections are relatively confident to 2050, we focused on uncertainties regarding intensity and frequency of storms and funding. Elicitation methods allowed us to make predictions in the absence of fully compatible models and to assess short- and long-term trade-offs. Specifically we addressed two questions. (1) Can collaborative decision analysis lead to consensus among a diverse set of decision makers responsible for environmental stewardship and faced with uncertainties about climate change, funding, and stakeholder values? (2) What is an optimal strategy for the conservation of tidal marshes, and what strategy is robust to the aforementioned uncertainties? We found that when taking this approach, consensus was reached among the stakeholders about the best management strategies to maintain tidal marsh integrity. A Bayesian decision network revealed that a strategy considering sea-level rise and storms explicitly in wetland restoration planning and designs was optimal, and it was robust to uncertainties about management effectiveness and budgets. We found that strategies that avoided explicitly accounting for future climate change had the lowest expected performance based on input from the team. Our decision-analytic framework is sufficiently general to offer an adaptable template, which can be modified for use in other areas that include a diverse and engaged stakeholder group.

California↗

Comparison of two benthic assemblage sampling gears for use on intertidal oyster reefs in Louisiana

Background Estuarine biodiversity plays a vital role in supporting ecosystem functions yet remains threatened by climate change and anthropogenic activity. Tracking and identifying estuarine biodiversity trends helps management ensure long-term provisions of human and environmental benefits by contributing to the estimation of habitat loss and the monitoring of restoration and conservation progress. However, the sampling gear and biodiversity metric used may indicate different conclusions, which can lead to uncertainty in the actual state of the ecosystem-level biodiversity. Sampling benthic biodiversity in complex estuarine habitats, such as oyster reefs, is particularly challenging because no one gear type captures entire target assemblages, and differences in gear efficiency on these complex habitats make comparisons across gear types challenging. Methods We investigated how estimates of oyster reef-associated benthic taxa abundance, richness, Pielou’s evenness, and Shannon-Wiener diversity differed across three Crassostrea virginica reefs in Louisiana between suction sampler and substrate tray sampling gears ( n = 6), and how gear influenced comparisons across reefs (3 reefs × 6 replicates × 2 gears). Results Abundance and richness were higher, and Pielou’s evenness was lower, in trays compared to suction samples at all reefs. Shannon-Wiener diversity was similar in suction samples and trays at two out of three reefs. Amphipod taxa were numerically dominant in trays, skewing the distribution of abundances and driving the reef assemblage differences between gears. Abundance and Shannon-Wiener diversity were similar across reefs within each gear. However, there were significant differences in richness across reefs in tray samples only, while evenness differed across reefs only in suction samples. Our results highlight that gear choices, along with biodiversity metrics tracked, can result in different conclusions in biodiversity trends, ultimately affecting conservation decisions and management.

Louisiana↗

Relative abundance and molecular evolution of Lake Sinai Virus (Sinaivirus) clades

Lake Sinai Viruses (Sinaivirus) are commonly detected in honey bees ( Apis mellifera ) but no disease phenotypes or fitness consequences have yet been demonstrated. This viral group is genetically diverse, lacks obvious geographic structure, and multiple lineages can co-infect individual bees. While phylogenetic analyses have been performed, the molecular evolution of LSV has not been studied extensively. Here, I use LSV isolates from GenBank as well as contigs assembled from honey bee Sequence Read Archive (SRA) accessions to better understand the evolutionary history of these viruses. For each ORF, substitution rate variation, codon usage, and tests of positive selection were evaluated. Outlier regions of high or low diversity were sought with sliding window analysis and the role of recombination in creating LSV diversity was explored. Phylogenetic analysis consistently identified two large clusters of sequences that correspond to the current LSV1 and LSV2 nomenclature, however lineages sister to LSV1 were the most frequently detected in honey bee SRA accessions. Different expression levels among ORFs suggested the occurrence of subgenomic transcripts. ORF1 and RNA-dependent RNA polymerase had higher evolutionary rates than the capsid and ORF4. A hypervariable region of the ORF1 protein-coding sequence was identified that had reduced selective constraint, but a site-based model of positive selection was not significantly more likely than a neutral model for any ORF. The only significant recombination signals detected between LSV1 and LSV2 initiated within this hypervariable region, but assumptions of the test (single-frame coding and independence of substitution rate by site) were violated. LSV codon usage differed strikingly from that of honey bees and other common honey-bee viruses, suggesting LSV is not strongly co-evolved with that host. LSV codon usage was significantly correlated with that of Varroa destructor , however, despite the relatively weak codon bias exhibited by the latter. While codon usage between the LSV1 and LSV2 clusters was similar for three ORFs, ORF4 codon usage was uncorrelated between these clades, implying rapid divergence of codon use for this ORF only. Phylogenetic placement and relative abundance of LSV isolates reconstructed from SRA accessions suggest that detection biases may be over-representing LSV1 and LSV2 in public databases relative to their sister lineages.

PeerJ↗

An updated genetic marker for detection of Lake Sinai Virus and metagenetic applications

Background Lake Sinai Viruses (LSV) are common RNA viruses of honey bees ( Apis mellifera ) that frequently reach high abundance but are not linked to overt disease. LSVs are genetically heterogeneous and collectively widespread, but despite frequent detection in surveys, the ecological and geographic factors structuring their distribution in A. mellifera are not understood. Even less is known about their distribution in other species. Better understanding of LSV prevalence and ecology have been hampered by high sequence diversity within the LSV clade. Methods Here we report a new polymerase chain reaction (PCR) assay that is compatible with currently known lineages with minimal primer degeneracy, producing an expected 365 bp amplicon suitable for end-point PCR and metagenetic sequencing. Using the Illumina MiSeq platform, we performed pilot metagenetic assessments of three sample sets, each representing a distinct variable that might structure LSV diversity (geography, tissue, and species). Results The first sample set in our pilot assessment compared cDNA pools from managed A. mellifera hives in California ( n = 8) and Maryland ( n = 6) that had previously been evaluated for LSV2, confirming that the primers co-amplify divergent lineages in real-world samples. The second sample set included cDNA pools derived from different tissues (thorax vs. abdomen, n = 24 paired samples), collected from managed A. mellifera hives in North Dakota. End-point detection of LSV frequently differed between the two tissue types; LSV metagenetic composition was similar in one pair of sequenced samples but divergent in a second pair. Overall, LSV1 and intermediate lineages were common in these samples whereas variants clustering with LSV2 were rare. The third sample set included cDNA from individual pollinator specimens collected from diverse landscapes in the vicinity of Lincoln, Nebraska. We detected LSV in the bee Halictus ligatus (four of 63 specimens tested, 6.3%) at a similar rate as A. mellifera (nine of 115 specimens, 7.8%), but only one H. ligatus sequencing library yielded sufficient data for compositional analysis. Sequenced samples often contained multiple divergent LSV lineages, including individual specimens. While these studies were exploratory rather than statistically powerful tests of hypotheses, they illustrate the utility of high-throughput sequencing for understanding LSV transmission within and among species.

California, Maryland, Nebraska↗

Northeast

The distinct seasonality of the Northeast’s climate supports a diverse natural landscape adapted to the extremes of cold, snowy winters and warm to hot, humid summers. This natural landscape provides the economic and cultural foundation for many rural communities, which are largely supported by a diverse range of agricultural, tourism, and natural resource-dependent industries (see Ch. 10: Ag & Rural, Key Message 4) . The recent dominant trend in precipitation throughout the Northeast has been towards increases in rainfall intensity, with increases in intensity exceeding those in other regions of the contiguous United States. Further increases in rainfall intensity are expected, with increases in total precipitation expected during the winter and spring but with little change in the summer. Monthly precipitation in the Northeast is projected to be about 1 inch greater for December through April by end of century (2070–2100) under the higher scenario (RCP8.5). Ocean and coastal ecosystems are being affected by large changes in a variety of climate-related environmental conditions. These ecosystems support fishing and aquaculture, tourism and recreation, and coastal communities. Observed and projected increases in temperature, acidification, storm frequency and intensity, and sea levels are of particular concern for coastal and ocean ecosystems, as well as local communities and their interconnected social and economic systems. Increasing temperatures and changing seasonality on the Northeast Continental Shelf have affected marine organisms and the ecosystem in various ways. The warming trend experienced in the Northeast Continental Shelf has been associated with many fish and invertebrate species moving northward and to greater depths. Because of the diversity of the Northeast’s coastal landscape, the impacts from storms and sea level rise will vary at different locations along the coast. Northeastern cities, with their abundance of concrete and asphalt and relative lack of vegetation, tend to have higher temperatures than surrounding regions due to the urban heat island effect. During extreme heat events, nighttime temperatures in the region’s big cities are generally several degrees higher than surrounding regions, leading to higher risk of heat-related death. Urban areas are at risk for large numbers of evacuated and displaced populations and damaged infrastructure due to both extreme precipitation events and recurrent flooding, potentially requiring significant emergency response efforts and consideration of a long-term commitment to rebuilding and adaptation, and/or support for relocation where needed. Much of the infrastructure in the Northeast, including drainage and sewer systems, flood and storm protection assets, transportation systems, and power supply, is nearing the end of its planned life expectancy. Climate-related disruptions will only exacerbate existing issues with aging infrastructure. Sea level rise has amplified storm impacts in the Northeast (Key Message 2), contributing to higher surges that extend farther inland, as demonstrated in New York City in the aftermath of Superstorm Sandy in 2012. Service and resource supply infrastructure in the Northeast is at increasing risk of disruption, resulting in lower quality of life, economic declines, and increased social inequality. Loss of public services affects the capacity of communities to function as administrative and economic centers and triggers disruptions of interconnected supply chains (Ch. 16: International, Key Message 1) . Increases in annual average temperatures across the Northeast range from less than 1°F (0.6°C) in West Virginia to about 3°F (1.7°C) or more in New England since 1901. Although the relative risk of death on very hot days is lower today than it was a few decades ago, heat-related illness and death remain significant public health problems in the Northeast. For example, a study in New York City estimated that in 2013 there were 133 excess deaths due to extreme heat. These projected increases in temperature are expected to lead to substantially more premature deaths, hospital admissions, and emergency department visits across the Northeast. For example, in the Northeast we can expect approximately 650 additional premature deaths per year from extreme heat by the year 2050 under either a lower (RCP4.5) or higher (RCP8.5) scenario and from 960 (under RCP4.5) to 2,300 (under RCP8.5) more premature deaths per year by 2090. Communities, towns, cities, counties, states, and tribes across the Northeast are engaged in efforts to build resilience to environmental challenges and adapt to a changing climate. Developing and implementing climate adaptation strategies in daily practice often occur in collaboration with state and federal agencies (e.g., New Jersey Climate Adaptation Alliance 2017, New York Climate Clearinghouse 2017, Rhode Island STORMTOOLS 2017, EPA 2017, CDC 2015 ). Advances in rural towns, cities, and suburban areas include low-cost adjustments of existing building codes and standards. In coastal areas, partnerships among local communities and federal and state agencies leverage federal adaptation tools and decision support frameworks (for example, NOAA’s Digital Coast, USGS’s Coastal Change Hazards Portal, and New Jersey’s Getting to Resilience). Increasingly, cities and towns across the Northeast are developing or implementing plans for adaptation and resilience in the face of changing climate (e.g., EPA 2017 ). The approaches are designed to maintain and enhance the everyday lives of residents and promote economic development. In some cities, adaptation planning has been used to respond to present and future challenges in the built environment. Regional efforts have recommended changes in design standards when building, replacing, or retrofitting infrastructure to account for a changing climate.

Report↗

Klamath-Siskiyou herpetofauna: Biogeographic patterns and conservation strategies

The Klamath-Siskiyou region of southwest Oregon and northwest California (USA) has some of the most complex landscape mosaics and plant communities in western North America, reflecting its marked diversity of precipitation and topography. With 38 native species of amphibians and reptiles, the Klamath-Siskiyou region has the most species-rich herpetofauna of any similarly sized mountain range in the Pacific Northwest. Although it is a biodiversity 'hot spot,' there are only two endemic species, both salamanders, in the Klamath-Siskiyou region. High diversity is due to the overlap of two major biogeographic groups: the Arcto- (= northern) and Madro- (= southern) Tertiary herpetofaunas. Many of the amphibians in the Klamath-Siskiyou region are restricted to specialized habitats. Much of our knowledge about the biology of the regional fauna is based on studies elsewhere. Distributional surveys and ecological research are needed to address how the herpetofauna responds to timber harvest and other human activities that may reduce populations and increase fragmentation of suitable habitats. Conservation of the region's diverse herpetofauna should emphasize strategies directed at habitat specialists and species at the latitudinal limits of their ranges.

California, Oregon↗

Vegetation assessment of forests of Pagan Island, Commonwealth of the Northern Mariana Islands

As part of the Marianas Expedition Wildlife Surveys-2010, the forest vegetation of the island of Pagan, Commonwealth of the Northern Mariana Islands (CNMI), was sampled with a series of systematic plots along 13 transects established for monitoring forest bird populations. Shrubland and grassland were also sampled in the northern half of the island. Data collected were woody plant density, tree diameter at breast height, woody plant density in height classes below 2 m, and ground cover measured with the point-intercept method. Coconut forests ( Cocos nucifera ) were generally found to have low native tree diversity, little regeneration of trees and shrubs in the forest understory, and little live ground cover. The sole exception was a coconut-dominated forest of the northeast side of the island that exhibited high native tree diversity and a large number of young native trees in the understory. Ironwood ( Casuarina equisetifolia ) forests on the northern half of the island were nearly monocultures with almost no trees other than ironwood in vegetation plots, few woody plants in the understory, and low ground cover dominated by native ferns. Mixed native forests of both northern and southern sections of the island had a diversity of native tree species in both the canopy and the sparse understory. Ground cover of native forests in the north had a mix of native and alien species, but that of the southern half of the island was dominated by native ferns and woody plants. During vegetation surveys in June–July 2010, 215 vascular plant species were observed on Pagan; 21 new island records of alien plants and 12 new island records of native plants were documented. Many of the new and recently sighted alien plants of the northern section of the island appeared to be in the incipient stage of invasion. Most of the new native plant sightings and a number of other rare tree and shrub species of Pagan were limited to forests of the rugged southern half of the island.

Pagan Island↗

Water transfer projects and the role of fisheries biologists

Water transfer projects are commonly considered important mechanisms for meeting increasing water demands. However, the movement of water from one area to another may have broad ecosystem effects, including on fisheries. The Southern Division of the American Fisheries Society held a symposium in 1995 at Virginia Beach, Virginia, to discuss the ecological consequences of water transfer and identify the role of fisheries biologists in such projects. Presenters outlined several case studies, including the California State Water Project, Garrison Diversion Project (North Dakota), Lake Texoma Water Transfer Project (Oklahoma-Texas), Santee-Cooper Diversion and Re-diversion projects (South Carolina), and Tri-State Comprehensive Study (Alabama-Florida-Georgia). Results from these studies suggest that fisheries biologists have provided critical information regarding potential ecological consequences of water transfer. If these professionals continue to be called for information regarding the ecological consequences of water transfer projects, developing a broader understanding of the ecological processes that affect the fish species they manage may be necessary. Although the traditional role of fisheries biologists has focused on the fishing customer base, fisheries management issues are only one component of the broad spectrum of ecosystem issues resulting from water transfer.

Conference Paper↗

Significance of nearshore trace-fossil assemblages of the cambro-ordovician deadwood formation and Aladdin Sandstone, South Dakota

The Cambro-Ordovician Deadwood Formation and Aladdin Sandstone represent intertidal and subtidal, nearshore deposystems that contain few well-preserved body fossils, but contain abundant trace fossils. The present study uses the much neglected trace-fossil fauna to describe the diverse paleoenvironments represented in the Deadwood-Aladdin deposystems, and to better understand the environmental conditions that controlled benthic life in the Early Paleozoic. The Deadwood-Aladdin ichnotaxa can be separated into three distinct assemblages based on the changing sedimentologic and hydrodynamic conditions that existed across the Cambro-Ordovician shelf. Trace-fossil assemblages and corresponding lithofacies characteristics indicate that the Deadwood-Aladdin deposystems formed within an intertidal-flat and subtidal-shelf environment. Based on the distribution and numbers of preserved ichnotaxa, the intertidal flat can be subdivided further into an ecologically stressful inner sand-flat environment, and a more normal marine outer sand-flat environment, both of which belong to a mixed, Skolithos-Cruziana softground ichnofacies. The inner sand flat is characterized by low diversity, low numbers, and a general lack of complexly constructed ichnotaxa. Trace fossils common to both assemblages tend to be smaller in the inner flat compared to the outer sand flat. Taphonomic effects, such as substrate type and sediment heterogeneity, also aid in differentiating between the inner and outer sand-flat assemblages. The subtidal shelf environment is categorized in the Cruziana Ichnofacies. Ichnological evidence of periodic tempestite deposition and hardground development within this subtidal regime is manifested by high diversity and low abundance of ichnogenera.

Annals of Carnegie Museum↗

Operational modeling system with dynamic-wave routing

A near real-time streamflow-simulation system utilizing continuous-simulation rainfall-runoff generation with dynamic-wave routing is being developed by the U.S. Geological Survey in cooperation with the Du Page County Department of Environmental Concerns for a 24-kilometer reach of Salt Creek in Du Page County, Illinois. This system is needed in order to more effectively manage the Elmhurst Quarry Flood Control Facility, an off-line stormwater diversion reservoir located along Salt Creek. Near real time simulation capabilities will enable the testing and evaluation of potential rainfall, diversion, and return-flow scenarios on water-surface elevations along Salt Creek before implementing diversions or return-flows. The climatological inputs for the continuous-simulation rainfall-runoff model, Hydrologic Simulation Program - FORTRAN (HSPF) are obtained by Internet access and from a network of radio-telemetered precipitation gages reporting to a base-station computer. The unit area runoff time series generated from HSPF are the input for the dynamic-wave routing model. Full Equations (FEQ). The Generation and Analysis of Model Simulation Scenarios (GENSCN) interface is used as a pre- and post-processor for managing input data and displaying and managing simulation results. The GENSCN interface includes a variety of graphical and analytical tools for evaluation and quick visualization of the results of operational scenario simulations and thereby makes it possible to obtain the full benefit of the fully distributed dynamic routing results.

Conference Paper↗

Evaluation of stream water quality in Atlanta, Georgia, and the surrounding region (USA)

A water-quality index (WQI) was developed from historical data (1986-1995) for streams in the Atlanta Region and augmented with 'new' and generally more comprehensive biweekly data on four small urban streams, representing an industrial area, a developed medium-density residential area and developing and developed low-density residential areas. Parameter WQIs were derived from percentile ranks of individual water-quality parameter values for each site by normalizing the constituent ranks for values from all sites in the area for a base period, i.e. 1990-1995. WQIs were developed primarily for nutrient-related parameters due to data availability. Site WQIs, which were computed by averaging the parameter WQIs, range from 0.2 (good quality) to 0.8 (poor quality), and increased downstream of known nutrient sources. Also, annual site WQI decreases from 1986 to 1995 at most long-term monitoring sites. Annual site WQI for individual parameters correlated with annual hydrological characteristics, particularly runoff, precipitation quantity, and water yield, reflecting the effect of dilution on parameter values. The WQIs of the four small urban streams were evaluated for the core-nutrient-related parameters, parameters for specific dissolved trace metal concentrations and sediment characteristics, and a species diversity index for the macro-invertebrate taxa. The site WQI for the core-nutrient-related parameters used in the retrospective analysis was, as expected, the worst for the industrial area and the best for the low-density residential areas. However, macro-invertebrate data indicate that although the species at the medium-density residential site were diverse, the taxa at the site were for species tolerant of degraded water quality. Furthermore, although a species-diversity index indicates no substantial difference between the two low-density residential areas, the number for macro-invertebrates for the developing area was much less than that for the developed area, consistent with observations of recent sediment problems probably associated with construction in the basin. However, sediment parameters were similar for the two sites suggesting that the routine biweekly measurements may not capture the short-term increases in sediment transport associated with rainstorms. The WQI technique is limited by the number and types of parameters included in it, the general conditions of those parameters for the range of conditions in area streams, and by the effects of external factors, such as hydrology, and therefore, should be used with caution.

IAHS-AISH Publication↗

Herpetofauna of the cedar glades and associated habitats of the Inner Central Basin of middle Tennessee

The cedar glades and barrens of the Inner Central Basin (ICB) of middle Tennessee support a unique and diverse flora and fauna and represent some of the state's most valued natural areas. We conducted herpetofaunal inventories of the cedar glades, associated barrens, cedar-hardwood forest, and adjacent aquatic habitats of the Stones River drainage of Middle Tennessee, focusing our sampling effort primarily at seven state- or federally owned properties in Rutherford and Wilson counties. These properties included Stones River National Battlefield (SRNB), Flat Rock State Natural Area (FRSNA), Vesta Cedar Glade State Natural Area (VSNA), Fall Creek Recreation Area (FCRA) on J. Percy Priest Wildlife Management Area, Cedars of Lebanon State Forest (CLSF), Cedars of Lebanon State Forest Natural Area (CLSNA), and Cedars of Lebanon State Park (CLSP). We used a variety of inventory techniques in terrestrial, aquatic, and subterranean habitats to survey these properties periodically from 1989 to 2010. We documented 49 species (22 amphibian and 27 reptile) accounting for 75.4% of the 65 herpetofaunal species thought to occur in the ICB, including records for Cemophora coccinea, Aneides aeneus, Gyrinophilus palleucus, Ambystoma barbouri, and Pseudotriton montanus. We found differences in alpha and beta diversity between sites, with the CLSF complex containing a high of 41 herpetofaunal species and FRSNA containing a low of 23 species. Beta diversity comparisons indicated similarity in amphibian species composition between FRSNA and CLSF and between SRNB and CLSF (9 shared species), and in reptile species composition between VSNA and the CLSF complex (16 shared species). We compare the results of our inventory with two previous studies conducted in the area and discuss the relative abundance, conservation, and threats to the herpetofaunal community of these habitats.

Herpetological Conservation and Biology↗

2011 Georgiana Slough non-physical barrier performance evaluation project report

The Sacramento River and its tributaries support populations of anadromous fish species including winter-run, spring-run, fall-run, and late fall–run Chinook salmon ( Oncorhynchus tshawytscha ); and steelhead ( O. mykiss ). Several of these species are listed as threatened or endangered under the California Endangered Species Act (CESA), federal Endangered Species Act (ESA), or both. These species spawn and rear in Sacramento River tributaries; adults use the mainstem Sacramento River for primarily upstream migration and juveniles use it for downstream migration. Juvenile Chinook salmon and steelhead migrate through the lower river during winter and spring. During their downstream migration, juvenile salmonids encounter alternative pathways, such as Sutter and Steamboat Sloughs, the Sacramento–San Joaquin Delta (Delta), Delta Cross Channel (DCC), and Georgiana Slough. Likewise, sturgeon juveniles migrate downstream in the Sacramento River basin to the Delta, utilizing the distributary channels to rear within and migrate through the system. Georgiana Slough is a natural channel that allows water and fish to move into the interior Delta. Previous studies have demonstrated that juvenile Chinook salmon experience greater mortality when migrating into Georgiana Slough than those juveniles that continue to migrate downstream in the Sacramento River (Perry 2010). Movement and/or diversion of these fish into the interior and south Delta increases the likelihood of losses through predation, entrainment into non-project Delta diversions, and mortality associated with the State Water Project (SWP) and Central Valley Project (CVP) pumping facilities in the south Delta (Perry 2010; NMFS 2009). Figure ES-1 shows the migration pathways in the lower Sacramento River and Delta for outmigrating anadromous salmonids, and the location of the DCC, and the SWP and CVP pumping facilities in the south Delta. Passage of juvenile salmonids from the Sacramento River into the interior Delta through the DCC can be reduced through seasonal closure of the radial gates (February through May); however, no similar protection is available to reduce the movement of juvenile salmonids from the Sacramento River into the interior Delta through Georgiana Slough. Flows into Georgiana Slough improve water quality and flushing in the interior Delta and free access encourages use by recreational boaters. Because of these benefits, alternatives to the installation of a physical barrier (i.e. radial gates), are being investigated. Under the ESA, the National Marine Fisheries Service (NMFS) issued the 2009 Biological and Conference Opinion for the Long‐Term Operations of the Central Valley Project and State Water Project (BO) for Chinook salmon and other listed anadromous fish species (NMFS 2009). Reasonable and Prudent Alternative (RPA) Action IV.1.3 of the BO requires the California Department of Water Resources (DWR) and the U.S. Bureau of Reclamation (Reclamation) to consider engineering solutions to reduce the diversion of juvenile salmonids from the Sacramento River into the interior and south Delta. DWR implemented the 2011 Georgiana Slough NonPhysical Barrier (GSNPB) Study to test the effectiveness of using a non-physical barrier, referred to as a behavioral Bio-Acoustic Fish Fence (BAFF), that combines three stimuli to deter juvenile Chinook salmon from entering Georgiana Slough: sound, high-intensity modulated light (previously known as stroboscopic light), and a bubble curtain. This report presents the results of the experimental tests conducted in 2011.

California↗

Mobile Bay

Mobile Bay is the largest bay found in Alabama’s coastal area (Handley et al., 2007). It was named an Estuary of National Significance in 1995 under the U.S. Environmental Protection Agency’s (EPA) National Estuary Program (NEP), and its Comprehensive Conservation Management Plan was completed in 2002. Mobile Bay is 1,070 km 2 (413 miles 2 ) in area and 51 km (32 miles) long, making it the sixth largest estuary in the continental United States (Mobile Bay NEP, 2008). Its ecosystem provides habitat for more than 300 species of birds, 310 species of fish, 68 species of reptiles, 57 species of mammals, 40 species of amphibians, and 15 species of shrimp (Mobile Bay NEP, 1997). Mobile Bay lies between the Mississippi and Atlantic Flyways (Mobile Bay NEP, 2003). Commercial and residential development and industrial use is heavy in the Mobile Bay area. Although local growth and industrial markets support the Mobile Bay area economy, the resulting environmental damage to the very ecosystem upon which they depend remains a threat to the environment, economy, and population. The Mobile Bay ecosystem boasts high biological diversity and productivity and supports many freshwater and saltwater species of recreational and commercial importance. The great diversity of Mobile Bay reflects the diversity of Alabama, which is home to the largest number of different plant and animal species of all states east of the Mississippi River (Stein, 2002), and is bolstered by the unique climate and geographic conditions surrounding the bay. Freshwater inflow from the Mobile-Tensaw River Delta, ranging from 60,000 to 3,700,000 gallons per second (Wallace, 1996), mixes with saltwater from the Gulf of Mexico, which enters Mobile Bay via wind and tides (Burgan and Engle, 2006). Because of the unique conditions surrounding Mobile Bay, including shallow waters, a dynamic climate, and artificial hydrologic modifications—such as the construction of the Mobile Bay Causeway in the 1920s, which serves as an unintentional barrier between Delta waters north of the Causeway and saline waters south of the Causeway, the salinity of Mobile Bay is highly variable. Mobile Bay receives an average of 165 cm (65 inches) of rain per year from tropical storms, summer thunderstorms, and winter cold fronts (Stout et al., 1998). The climate and geography that have made Mobile Bay so rich in resources have also contributed to the threats surrounding its ecosystem. The extensive amount of rain in Mobile Bay creates large amounts of runoff, polluting the waters with fertilizers, chemicals, sediment, oil, trash, and sewage (Mobile Bay NEP, 1997). Tourism, ecotourism, recreational and commercial fishing, recreational boating, shipping, and chemical, pulp, and paper production are significant industries in Mobile Bay and the surrounding areas. Despite the approximate \$3 billion and 55,000 jobs these industries bring into the community (Alabama Tourism Department, 2010), the growth, development, and environmental stress they create are major threats to the Mobile Bay ecosystem. Among the nation’s states, Alabama ranks fifth in number of different species (144 endemic species), second in number of extinctions that have already occurred (90 extinct species) and fourth in number of species at risk for extinction (14.8% at risk out of 4,533 total species; Stein, 2002). Twenty-one of these threatened and endangered species are found in Mobile Bay, whose brackish waters provide a nursery area for many species of vertebrates and invertebrates. Some of these species include the Alabama sturgeon, Gulf sturgeon, heavy pigtoe mussel, inflated heel-splitter mussel, West Indian manatee, Alabama beach mouse, Perdido beach mouse, Alabama red-bellied turtle, gopher tortoise, Kemp’s ridley sea turtle, green sea turtle, loggerhead sea turtle, eastern indigo snake, flatwoods salamander, piping plover, red-cockaded woodpecker, and wood stork. Habitat loss underlies the decline of some bird species in Mobile Bay, and large mammals such as the red wolf, Florida panther, and Florida black bear are no longer found in the area. However, some rare species, such as the swallow-tailed kite, sandhill crane, and gopher tortoise can still be found (Duke and Kruczynski, 1992). The value of wetlands in Mobile Bay and the rest of the Gulf of Mexico is still being investigated. Although various monetary valuations of wetlands exist, critics remark that undervaluation of wetlands is inevitable (Mobile Bay NEP, 2008) and that estimates often do not place appropriate value on ecological services (Mitsch and Gosselink, 2000). Additionally, many estimates account only for anthropogenic values. One estimate concludes that one acre of wetlands performs \$3,000 worth of water purification each year (Mobile Bay NEP, 1997). With more than 76,890 hectares (190,000 acres) of wetlands in the Mobile Bay area, that equates to a value exceeding one-half billion dollars every year. Tourism, fishing, boating, production, and shipping are significant industries in the Mobile Bay area. More than 90% of fish landed in recreational and commercial fishing in the bay depend on bay habitat, including wetlands, for life requirements (Mobile Bay NEP, 1997). The Port of Mobile is Alabama’s only ocean-ship port (Mobile Bay NEP, 2008). Baldwin County, on the eastern side of the bay, experienced a population increase of 75% from 1990 to 2007, with an 89% increase in housing units (Mobile Bay NEP, 2008). Development and industry support the Mobile Bay economy, but they depend on the continued health, sustainability, and production of the water and living resources of the Mobile Bay ecosystem. Wetland loss, along with other forms of environmental degradation, remains a threat to the Mobile Bay ecosystem and Mobile Bay’s socioeconomic foundation.

Alabama↗