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Le stereorestituteur Kelsh

Le stéréorestituteur Kelsh est un appareil de restitution à double projection basé sur le principe des anaglyphes, comme le Multiplex par exemple, mais il a une plus grande précision que ce dernier, vu que les négatifs sont utilisés directement pour la restitution, sans que l'on soit obligé de les réduire au préalable. Une telle solution devient possible lorsque l'échelle de l'image plastique (modèle) est au moins 7 fois plus grande que celle des clichés. L'appareil comprend un support reposant par 4 pieds sur la table à dessin. Sa partie supérieure porte, par l'intermédiaire de trois vis calantes, un cadre dans lequel les deux chambres de projection sont suspendues. Des leviers de commande, agissant sur les chambres, permettent d'introduire la base, le déversement et les inclinaisons transversale et longitudinale. Les clichés conjugués ou des diapositifs sont directement placés dans les chambres de projection munies d'objectifs. En projetant les deux clichés au moyen de couleurs complémentaires, l'observateur — muni de lunettes à verres colorés (couleurs complémentaires) — observe l'image plastique audessus de la table à dessin, l'orientation relative des chambres ayant été établie au préalable. Pour le report des points de l'image plastique sur la minute, l'opérateur dispose d'une tablette amovible à marque-repère lumineuse qu'il déplace à la main et dont la hauteur au-dessus de la table à dessin peut être commandée par une molette. Pour établir l'orientation absolue de l'image plastique, il suffit d'incliner convenablement le cadre de suspension à l'aide des vis calantes, l'orientation relative n'étant pas détruite par cette opération. Les deux clichés sont éclairés par des projecteurs munis d'une suspension à la cardan et reliés à la tablette de restitution par des tiges télescopiques. Moyennant ce dispositif, l'éclairage est concentré sur deux petites régions conjuguées des clichés et l'observateur ne voit ainsi qu'une petite partie de l'image spatiale, ce qui présente l'avantage de ne pas l'éblouir. D'autre part, les tiges télescopiques impriment, suivant l'inclinaison, un déplacement vertical plus ou moins prononcé aux objectifs de projection, ce qui permet d'éliminer les erreurs de distorsion. Pour les travaux de triangulation aérienne, l'auteur propose d'appliquer la méthode des plaques à fontes radiales (templets). Pour le passage d'un modèle à l'autre, l'orientation spatiale des chambres de restitution est déterminée au moyen d'une nivelle placée sur les clichés. Un premier essai a été effectué sur 7 modèles (échelle des clichés 1/34000) et après compensation l'erreur probable altimétrique des douze points connus s'est élevée à 1.50 m tandis que l'erreur maximum était de 4 m. L'erreur altimétrique des restitutions effectuées par le U.S. Forest Service en Californie avec des clichés ou 1/48000, où les différences de niveau dépassaient parfois 600 m par cliché, varie entre 1 m et 2.70 m.

Photogrammetria↗

Toxicity of acidified chitosan for cultured rainbow trout (Oncorhynchus mykiss)

Chitosan is a deacetylation product of chitin. It is used as a flocculent for sewage and brewery wastes, and as a chelator of heavy metals. In aquaculture, chitosan has been used as an immunostimulant for protection against bacterial diseases in fish, for controlled release of vaccines, and as a diet supplement. Chitosan has generally been considered to be nontoxic to animals, but when it was dissolved in acetic acid and added to a culture system at 1.0 ppm to remove organic solids, we found acute toxicity to rainbow trout ( Oncorhynchus mykiss ). In controlled experiments to determine the extent of toxicity, we found that trout died after several hours exposure to 0.75 ppm and died in 24 h after exposure to 0.075 ppm. Exposure to 0.038 ppm resulted in mortality after 6 days exposure, while exposure to 0.019 ppm resulted in no mortality after 14 days exposure. Histological examination of gills, skin, muscle, and internal organs indicated significant and consistent pathological changes only in gills. Lifting of lamellar epithelium, hypertrophy and hyperplasia of lamellar epithelial cells occurred in trout exposed to 0.019 and 0.038 ppm. In trout exposed to 0.75 or 0.075 ppm chitosan, large areas of lamellar fusion were observed. These results show that soluble acidified chitosan is highly toxic to rainbow trout even at low concentrations.

Aquaculture↗

Flow cytometric evaluation of antibiotic effects on viability and mitochondrial function of refrigerated spermatozoa of Nile tilapia

Improved techniques for storage and evaluation of fish sperm would enhance breeding programs around the world. The goal of this study was to test the effect of antibiotics on refrigerated sperm from Nile tilapia (Oreochromis niloticus) by use of flow cytometry with 2 dual-staining protocols for objective assessment of sperm quality. Concentrations of 1 x 109 sperm/mL were suspended in Ringer's buffer at 318 mOsmol/kg (pH 8.0). The fluorescent stains Sybr 14 (10 ??M), propidium iodide (2.4 mM), and rhodamine 123 (0.13 ??M) were used to assess cell viability and mitochondrial function. Three concentrations of ampicillin, gentamicin, and an antibiotic/antimycotic solution were added to fresh spermatozoa. Motility estimates and flow cytometry measurements were made daily during 7 d of refrigerated storage (4 ??C). The highest concentrations of gentamicin and antibiotic/antimycotic and all 3 concentrations of ampicillin significantly reduced sperm viability. The highest of each of the 3 antibiotic concentrations significantly reduced mitochondrial function. This study demonstrates that objective sperm quality assessments can be made using flow cytometry and that addition of antibiotics at appropriate concentrations can lengthen refrigerated storage time for tilapia spermatozoa. With minor modifications, these protocols can be adapted for use with sperm from other species and with other tissue types.

Theriogenology↗

Role of selenium toxicity and oxidative stress in aquatic birds

Adverse effects of selenium (Se) in wild aquatic birds have been documented as a consequence of pollution of the aquatic environment by subsurface agricultural drainwater and other sources. These effects include mortality, impaired reproduction with teratogenesis, reduced growth, histopathological lesions and alterations in hepatic glutathione metabolism. A review is provided, relating adverse biological effects of Se in aquatic birds to altered glutathione metabolism and oxidative stress. Laboratory studies, mainly with an organic form of Se, selenomethionine, have revealed oxidative stress in different stages of the mallard (Anas platyrhynchos) life cycle. As dietary and tissue concentrations of Se increase, increases in plasma and hepatic GSH peroxidase activities occur, followed by dose-dependent increases in the ratio of hepatic oxidized to reduced glutathione (GSSG:GSH) and ultimately hepatic lipid peroxidation measured as an increase in thiobarbituric acid reactive substances (TBARS). One or more of these oxidative effects were associated with teratogenesis (4.6 ppm wet weight Se in eggs), reduced growth in ducklings (15 ppm Se in liver), diminished immune function (5 ppm Se in liver) and histopathological lesions (29 ppm Se in liver) in adults. Manifestations of Serelated effects on glutathione metabolism were also apparent in field studies in seven species of aquatic birds. Reduced growth and possibly immune function but increased liver:body weight and hepatic GSSG:GSH ratios were apparent in American avocet (Recurvirostra americana) hatchlings from eggs containing 9 ppm Se. In blacknecked stilts (Himantopus mexicanus), which contained somewhat lower Se concentrations, a decrease in hepatic GSH was apparent with few other effects. In adult American coots (Fulica americana), signs of Se toxicosis included emaciation, abnormal feather loss and histopathological lesions. Mean liver concentrations of 28 ppm Se (ww) in the coots were associated with elevated hepatic GSH peroxidase, depletion of hepatic protein bound thiols and total thiols, but a small increase in GSH. Diving ducks in the San Francisco Bay area exhibited a positive correlation between hepatic Se concentration and GSH peroxidase activity (r=0.63, P<0.05), but a negative correlation between hepatic Se and GSH concentration (r=0.740, P<0.05). In willets (Catoptrophorus semipalmatus) from the San Diego area, positive correlations occurred between hepatic Se concentration and GSSG (r=0.70, P<0.001), GSSG:GSH ratio, and TBARS. In emperor geese (Chen canagica) from western Alaska, blood levels of up to 9.4 ppm occurred and were associated with increased plasma GSH peroxidase activity (r=0.62, P<0.001), but with decreased plasma GSSG reductase activity. When evaluating Se toxicity, interactive nutritional factors, including other elements and dietary protein, should also be taken into consideration. Further studies are needed to examine the relationship between different forms of environmentally occurring selenium, arsenic and mercury on reproduction, hepatotoxicity and immune function of aquatic birds. Further selenium nutritional interaction studies may also help to illucidate the mechanism of selenium induced teratogenesis, by optimizing GSH and other antioxidant defense mechanisms in a manner that would stabilize or raise the cell's threshold for susceptibility to toxic attack from excess selenium. It is concluded that Se-related manifestations of oxidative stress may serve as useful bioindicators of Se exposure and toxicity in wild aquatic birds.

Aquatic Toxicology↗

Probabilistic assessment methodology for continuous-type petroleum accumulations

The analytic resource assessment method, called ACCESS (Analytic Cell-based Continuous Energy Spreadsheet System), was developed to calculate estimates of petroleum resources for the geologic assessment model, called FORSPAN, in continuous-type petroleum accumulations. The ACCESS method is based upon mathematical equations derived from probability theory in the form of a computer spreadsheet system. ?? 2003 Elsevier B.V. All rights reserved.

International Journal of Coal Geology↗

Habitat evaluation using GIS a case study applied to the San Joaquin Kit Fox

Concern over the fate of plant and animal species throughout the world has accelerated over recent decades. Habitat loss is considered the main culprit in reducing many species' abundance and range, leading to numerous efforts to plan and manage habitat preservation. Our work uses Geographic Information Systems (GIS) data and modeling to define a spatially explicit analysis of habitat value, using the San Joaquin Kit Fox (Vulpes macrotis mutica) of California (USA) as an example. Over the last 30 years, many field studies and surveys have enhanced our knowledge of the life history, behavior, and needs of the kit fox, which has been proposed as an umbrella or indicator species for grassland habitat in the San Joaquin Valley of California. There has yet been no attempt to convert much of this field knowledge into a model of spatial habitat value useful for planning purposes. This is a significant omission given the importance and visibility of the imperiled kit fox and increasing trends toward spatially explicit modeling and planning. In this paper we apply data from northern California to derive a small-cell GIS raster of habitat value for the kit fox that incorporates both intrinsic habitat quality and neighborhood context, as well the effects of barriers such as roads. Such a product is a useful basis for assessing the presence and amounts of good (and poor) quality habitat and for eventually constructing GIS representations of viable animal territories that could be included in future reserves. ?? 2001 Elsevier Science B.V.

Landscape and Urban Planning↗

Heavy mineral provinces of the Palos Verdes margin, southern California

Natural sources of sediment for the Palos Verdes margin, southern California, have been augmented by effluent discharged from Los Angeles County Sanitation District's sewage-treatment facility and by the reactivation of the Portuguese Bend landslide. Heavy minerals in very fine and fine sand (63-250 ??m) from beach and shelf sites off the Palos Verdes Peninsula distinguish effluent-affected sediment from unaffected deposits, and track the sediment contributed by the Portuguese Bend landslide. Heavy minerals also identify heterogeneous sediment sources for the nearshore zone and relate outer-shelf sediment to depositional cells north and south of the area.

Continental Shelf Research↗

Modeling white sturgeon movement in a reservoir: The effect of water quality and sturgeon density

We developed a movement model to examine the distribution and survival of white sturgeon (Acipenser transmontanus) in a reservoir subject to large spatial and temporal variation in dissolved oxygen and temperature. Temperature and dissolved oxygen were simulated by a CE-QUAL-W2 model of Brownlee Reservoir, Idaho for a typical wet, normal, and dry hydrologic year. We compared current water quality conditions to scenarios with reduced nutrient inputs to the reservoir. White sturgeon habitat quality was modeled as a function of temperature, dissolved oxygen and, in some cases, suitability for foraging and depth. We assigned a quality index to each cell along the bottom of the reservoir. The model simulated two aspects of daily movement. Advective movement simulated the tendency for animals to move toward areas with high habitat quality, and diffusion simulated density dependent movement away from areas with high sturgeon density in areas with non-lethal habitat conditions. Mortality resulted when sturgeon were unable to leave areas with lethal temperature or dissolved oxygen conditions. Water quality was highest in winter and early spring and lowest in mid to late summer. Limiting nutrient inputs reduced the area of Brownlee Reservoir with lethal conditions for sturgeon and raised the average habitat suitability throughout the reservoir. Without movement, simulated white sturgeon survival ranged between 45 and 89%. Allowing movement raised the predicted survival of sturgeon under all conditions to above 90% as sturgeon avoided areas with low habitat quality. ?? 2003 Elsevier B.V. All rights reserved.

Ecological Modelling↗

Osmoregulatory actions of the GH/IGF axis in non-salmonid teleosts

Salmonid fishes provided the first findings on the influence of the growth hormone (GH)/insulin-like growth factor I (IGF-I) axis on osmoregulation in teleost fishes. Recent studies on non-salmonid species, however, indicate that this physiological action of the GH/IGF-I axis is not restricted to salmonids or anadromous fishes. GH-producing cells in the pituitary of fish acclimated to different salinities show different degrees of activation depending on the species studied. Plasma GH levels either increase or do not change after transfer of fish from freshwater to seawater. Treatment with GH or IGF-I increases salinity tolerance and/or increases gill Na + ,K + -ATPase activity of killifish ( Fundulus heteroclitus ), tilapia ( Oreochromis mossambicus and Oreochromis niloticus ) and striped bass ( Morone saxatilis ). As in salmonids, a positive interaction between GH and cortisol for improving hypoosmoregulatory capacity has been described in tilapia ( O . mossambicus ). Research on the osmoregulatory role of the GH/IGF-I axis is derived from a small number of teleost species. The study of more species with different osmoregulary patterns will be necessary to fully clarify the osmoregulatory role of GH/IGF-I axis in fish. The available data does suggest, however, that the influence of the GH/IGF-I axis on osmoregulation may be a common feature of euryhalinity in teleosts.

Comparative Biochemistry and Physiology, Part B: B↗

Putting aquifers into atmospheric simulation models: An example from the Mill Creek Watershed, Northeastern Kansas

Aquifer-atmosphere interactions can be important in regions where the water table is shallow (<2 m). A shallow water table provides moisture for the soil and vegetation and thus acts as a source term for evapotranspiration to the atmosphere. A coupled aquifer-land surface-atmosphere model has been developed to study aquifer-atmosphere interactions in watersheds, on decadal timescales. A single column vertically discretized atmospheric model is linked to a distributed soil-vegetation-aquifer model. This physically based model was able to reproduce monthly and yearly trends in precipitation, stream discharge, and evapotranspiration, for a catchment in northeastern Kansas. However, the calculated soil moisture tended to drop to levels lower than were observed in drier years. The evapotranspiration varies spatially and seasonally and was highest in cells situated in topographic depressions where the water table is in the root zone. Annually, simulation results indicate that from 5-20% of groundwater supported evapotranspiration is drawn from the aquifer. The groundwater supported fraction of evapotranspiration is higher in drier years, when evapotranspiration exceeds precipitation. A long-term (40 year) simulation of extended drought conditions indicated that water table position is a function of groundwater hydrodynamics and cannot be predicted solely on the basis of topography. The response time of the aquifer to drought conditions was on the order of 200 years indicating that feedbacks between these two water reservoirs act on disparate time scales. With recent advances in the computational power of massively parallel supercomputers, it may soon become possible to incorporate physically based representations of aquifer hydrodynamics into general circulation models (GCM) land surface parameterization schemes. ?? 2002 Elsevier Science Ltd. All rights reserved.

Advances in Water Resources↗

Biogeochemical sulphur cycle in an extreme environment - Life beneath a high arctic glacier, Nunavut, Canada

Unique springs discharge from the surface of a high arctic glacier, releasing H2S, and depositing native sulphur, gypsum, and calcite. A rare CaCO3 polymorph, vaterite, is also observed. Physical and chemical conditions of the spring water and surrounding environment, as well as mineralogical and isotopic signatures, argue for biologically mediated redox reactions controlling sulfur. Cell counts and DNA analyses, confirm bacteria are present in the spring system. ?? 2003 Elsevier Science B.V. All rights reserved.

Conference Paper↗

Deformities, PCBs, and TCDD-equivalents in double-crested cormorants ( Phalacrocorax auritus ) and Caspian terns ( Hydroprogne caspia ) of the Upper Great Lakes 1986–1991: Testing a cause-effect hypothesis

Deformities have been reported in many species of colonial waterbirds from several localities on the Laurentian Great Lakes. The hypothesis that deformities were caused by either polychlorinated biphenyls (PCBs) or contaminants measured as 2,3,7,8-tetrachlorodibenzo- p -dioxin equivalents (TCDD-EQs) is tested in this review of available data on concentrations of contaminants in eggs and observed deformities in embryos and chicks of double-crested cormorants ( Phalacrocorax auritus ) and Caspian terns ( Hydroprogne caspia ) between 1986 and 1991. Hatched chicks, live and dead eggs retrieved from 37 colonies in the upper Great Lakes were assessed for gross anatomical deformities. Rates of embryo death from seven regions of the upper Great Lakes were measured annually between 1986–1991. Half the embryos found dead in eggs were deformed. Nineteen types of abnormalities or deformities were observed. Subcutaneous edema in cormorants and gastroschisis in terns were the most common abnormalities in live or dead eggs. One of ten crossed-billed cormorant embryos survived to hatch. No bill-deformed terns hatched, although tern embryos had a greater rate of crossed-bills than cormorants. The suite of deformities and abnormalities found was similar to that produced in chickens by exposure to planar polychlorinated biphenyl (pPCB) and dioxin congeners. Hatching and deformity rates were correlated with concentrations ofpPCBs and TCDD-EQs. Planar PCB congeners that contributed most of the TCDD-EQs were present at concentrations sufficient to cause the observed effects. TCDD-EQs measured by H4IIE rat hepatoma cell 7-ethoxyresorufin O-deethylase (EROD) bioassay were highly correlated with deformity rates observed in cormorant chicks, live and dead eggs, and egg death rates. Similar correlations of TCDD-EQs with deformity rates were found in hatched tern chicks, dead eggs, and egg death rates, but not in live eggs. TCDD-EQs were more highly correlated to deformity and embryo death rates than total PCBs. The weight of evidence and these data are sufficient to reject the null hypothesis that there is no causal relationship between the incidence of deformities in cormorants and terns and exposure to planar halogenated compounds measured as TCDD-EQs or total PCBs in the Great Lakes.

Journal of Great Lakes Research↗

Mobility of 2,2',5,5'-tetrachlorobiphenyl in model systems containing bottom sediments and water from the lower Fox River, Wisconsin

Sediment-water partitioning and diffusive transport of 2,2’,5,5’-tetrachlorobiphenyl, PCB congener IUPAC #52 (TCB52) were examined in laboratory experiments with sediments from two sites in the lower Fox River, Wisconsin. Native water was pumped at controlled flow rates through cells containing sediments amended with a known activity of carbon-14-labeled TCB52. Concentrations of TCB52 in water and sediments were determined by liquid scintillation measurements of carbon-14 activity. Sediment-water partitioning was independent of flow rate for rates up to 8 m/d. Distribution coefficients (K d ) and soil-sorption coefficients (K oc ) were found to be at maximum levels 5–10 cm below the surface, despite an absence of significant variation in the fraction of organic carbon (f oc ) through the same profile. Other factors, such as the effects of colloids and microbial activity in the sediments, are likely to be important in controlling the PCB distribution. Log K d and log K oc ranges were 4.1–4.9 and 5.3–6.1, respectively, and calculated effective diffusivities at the sediment-water interface ranged from 3 to 8 × 10 −10 cm 2 /s. Gradual increases with time in TCB52 concentrations in the water phase, possibly due to effects of microbial activity, were observed. Diffusion experiments and models showed that the TCB52 migration rate within the sediment column is 8-9 mm/yr.

Journal of Great Lakes Research↗

In vivo and in vitro transfer of trout spleen sections for early analysis of the immune response

To determine the earliest time after in vivo immunisation that the spleen could be excised and held in vitro to detect an immune response, lake trout ( Salvelinus namaycush ) were exposed to DNP-Ficoll or Yersinia ruckeri O antigen administered by intraperitoneal injection or by bath. The spleens were excised from the fish at selected times after immunisation, placed in vitro and held in tissue culture media at 14°C until 10 days after the in vivo immunisation. They then were analysed for an immune response by counting the numbers of plaqueforming cells (PFC). PFC were first detected in samples taken 3 days after injection with either antigen. With bath immunisation, however, PFC were first seen 6 days post-immunisation when using the Y. ruckeri O antigen and no PFC above background levels appeared in fish bathed in the DNP-Ficoll solution. On day 10 after immunisation, the spleens taken directly from immunised fish always produced more PFC than when the spleens were taken through in vitro culture. A unique feature of this method, is that an immune response to an antigen may be detected by excising the spleen and holding it in in vitro culture without necessarily holding the fish for long periods of time after antigen injection or bath. This technique also adds more information on how rapidly the bacterins or antigens are processed by fish to initiate an immune response.

Fish and Shellfish Pathology↗

Status and opportunities for genomics research with rainbow trout

The rainbow trout (Oncorhynchus mykiss) is one of the most widely studied of model fish species. Extensive basic biological information has been collected for this species, which because of their large size relative to other model fish species are particularly suitable for studies requiring ample quantities of specific cells and tissue types. Rainbow trout have been widely utilized for research in carcinogenesis, toxicology, comparative immunology, disease ecology, physiology and nutrition. They are distinctive in having evolved from a relatively recent tetraploid event, resulting in a high incidence of duplicated genes. Natural populations are available and have been well characterized for chromosomal, protein, molecular and quantitative genetic variation. Their ease of culture, and experimental and aquacultural significance has led to the development of clonal lines and the widespread application of transgenic technology to this species. Numerous microsatellites have been isolated and two relatively detailed genetic maps have been developed. Extensive sequencing of expressed sequence tags has begun and four BAC libraries have been developed. The development and analysis of additional genomic sequence data will provide distinctive opportunities to address problems in areas such as evolution of the immune system and duplicate genes. ?? 2002 Elsevier Science Inc. All rights reserved.

Conference Paper↗

Fires, floods and other extreme events – How watershed processes under climate change will shape our coastlines

Ongoing sea-level rise has brought renewed focus on terrestrial sediment supply to the coast because of its strong influence on whether and how long beaches, marshes and other coastal landforms may persist into the future. Here, we summarise findings of sediment discharge from several coastal rivers, revealing that infrequent, large-magnitude events have disproportionate influence on the morphodynamics of coastal landforms and littoral cells. These event-dominated effects are most pronounced for small, steep mountainous rivers that supply beach and wetland sediment along the world’s active tectonic margins, although infrequent events are important drivers of sediment discharge for rivers worldwide. Additionally, extreme events (recurrence intervals of decades to centuries) that follow wildfires, earthquakes, volcanic eruptions, extreme precipitation or – most notably – combinations of these factors can redefine coastal sediment budgets and morphology. Some of these extreme events (e.g., wildfires plus rainfall) are increasing in magnitude and frequency under modern climate warming, with the likely result of increasing sediment flux to affected coastlines. Climate change is also altering watershed processes in both high latitudes and high altitudes, resulting in increased sediment supply to downstream catchments. We conclude that sediment inputs to coastal systems are highly variable with time, and that the variability and trends in sediment input are as important to characterise as long-term averages.

Cambridge Prisms: Coastal Futures↗

Monitoring interdecadal coastal change along dissipative beaches via satellite imagery at regional scale

Coastal morphological changes can be assessed using shoreline position observations from space. However, satellite-derived waterline (SDW) and shoreline (SDS; SDW corrected for hydrodynamic contributions and outliers) detection methods are subject to several sources of uncertainty and inaccuracy. We extracted high-spatiotemporal-resolution (~50 m-monthly) time series of mean high water shoreline position along the Columbia River Littoral Cell (CRLC), located on the US Pacific Northwest coast, from Landsat missions (1984–2021). We examined the accuracy of the SDS time series along the mesotidal, mildly sloping, high-energy wave climate and dissipative beaches of the CRLC by validating them against 20 years of quarterly in situ beach elevation profiles. We found that the accuracy of the SDS time series heavily depends on the capability to identify and remove outliers and correct the biases stemming from tides and wave runup. However, we show that only correcting the SDW data for outliers is sufficient to accurately measure shoreline change trends along the CRLC. Ultimately, the SDS change trends show strong agreement with in situ data, facilitating the spatiotemporal analysis of coastal change and highlighting an overall accretion signal along the CRLC during the past four decades.

Oregon, Washington↗

Status assessment of the Endangered snow leopard Panthera uncia and other large mammals in the Kyrgyz Alay , using community knowledge corrected for imperfect detection

The Endangered snow leopard Panthera uncia occurs in the Central Asian Mountains, which cover c. 2 million km 2 . Little is known about its status in the Kyrgyz Alay Mountains, a relatively narrow stretch of habitat connecting the southern and northern global ranges of the species. In 2010 we gathered information on current and past (1990, the last year of the Soviet Union) distributions of snow leopards and five sympatric large mammals across 14,000 km 2 of the Kyrgyz Alay. We interviewed 95 key informants from local communities. Across 49 400-km 2 grid cells we obtained 1,606 and 962 records of species occurrence (site use) in 1990 and 2010, respectively. The data were analysed using the multi-season site occupancy framework to incorporate uncertainty in detection across interviewees and time periods. High probability of use by snow leopards in the past was recorded in > 70% of the Kyrgyz Alay. Between the two sampling periods 39% of sites showed a high probability of local extinction of snow leopard. We also recorded high probability of local extinction of brown bear Ursus arctos (84% of sites) and Marco Polo sheep Ovis ammon polii (47% of sites), mainly in regions used intensively by people. Data indicated a high probability of local colonization by lynx Lynx lynx in 41% of the sites. Although wildlife has declined in areas of central and eastern Alay, regions in the north-west, and the northern and southern fringes appear to retain high conservation value.

Oryx↗