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Observational, virological, and serological data provide insights into an outbreak of highly pathogenic avian influenza among wild birds on the Yukon-Kuskokwim Delta, Alaska in 2022

In 2021–22, clade 2.3.4.4b highly pathogenic avian influenza (HPAI) viruses were introduced by wild birds into North America, leading to geographically widespread disease. In response to HPAI outbreaks throughout late 2021 and early 2022, we recorded observations of sick and dead birds, estimated abundance of carcasses, collected swab and sera samples to detect viruses, and monitored bird nesting on the Yukon-Kuskokwim Delta region of Alaska to document potential effects of disease. Thirty-six reports of sick and dead birds were registered across the region. Nineteen carcasses were opportunistically collected for diagnostic testing, of which 12 were confirmed to be infected with clade 2.3.4.4b HPAI viruses. Carcass abundance estimates from line-distance sampling provided evidence that the most common species of dead birds from the western Yukon-Kuskokwim Delta region were Cackling Goose ( Branta hutchinsii minima ), Glaucous Gull ( Larus hyperboreus ), and Black Brant ( Branta bernicla nigricans ). Only one paired cloacal and oropharyngeal swab sample from a Northern Pintail ( Anas acuta ) tested positive for clade 2.3.4.4b HPAI virus, out of 464 live-captured duck and goose samples. Of 195 sera samples from waterfowl screened for antibodies reactive to influenza A viruses, antibodies were found in 41–98% of samples collected from Emperor Goose ( Anser canagicus ), Cackling Goose, Black Brant, and Spectacled Eider ( Somateria fischeri ). In addition, 15–98% of the same sera samples were reactive to a clade 2.3.4.4b H5 antigen. Fewer Black Brant and Emperor Goose nests were found on long-term study plots during 2022 than in previous years. Collectively, we found that HPAI viruses affected at least seven species of wild birds inhabiting the region during 2022. The full scope of impacts of HPAI at this location during 2022 is unknown, but our data indicate that acute effects to avian population health on the Yukon-Kuskokwim Delta region were likely modest.

Alaska↗

The epizootiology of type C botulism in fish-eating birds at Salton Sea, California

During 1996, type C avian botulism killed over 15,000 fish-eating birds at the Salton Sea in southern California. Amont those affected were nearly 10,000 western white pelicans (Pelecanus erythrorhynchos) and over 1,200 endangered California brown pelicans (Pelecanus occidentalis californicus). Since 1996, smaller epizootics have occurred every year. Type C botulism is not typically associated with fish-eating birds. In the case of the Salton Sea, Mozambique tilapia (Oreochromis mossambicus) are the suspected source of type C toxin, although the mechanism by which the fish acquire the toxin is still unknown. The goals of this study were to: 1) Determine presence/absence of active Clostridium botulinum type C and type C botulinum toxin in tilapia in the Salton Sea. 2) Use geospatial analyses to evaluate relationships between patterns of mortality in birds and fish and presence/absence of toxin and/or toxin-producing bacteria in sediments and fish. We investigated a method of detecting C. botulinum type C cells in the intestinal contents of Mozambique tilapia. This method involved extraction of predominantly cellular DNA and uses a polymerase chain reaction assay to detect presence of type C toxin gene. We collected sick, dead and healthy fish from various sites throughout the Sea during the summers of 1999 to 2001 in order to test them for the presence of active C. botulinum type C by PCR and for the presence of type C toxin by ELISA and mouse test. The results demonstrate that the tilapia population in the Salton Sea harbors C. botulinum type C cells within their gastrointestinal tract and the prevalence of this organism varies from year to year. The total number of fish with toxin-producing bacteria was significantly greater in 2000 than in 2001. No difference in the numbers of positives was detected between sick and dead fish compared to live fish, and there were no differences noted with regard to location of fish collection. The prevalence of active type C toxin in tilapia was low and the majority of samples positive for toxin by ELISA were negative on mouse test. Further investigation is currently underway. From 1999-2001, routine surveillance was conducted July through November to detect morbidity and mortality in pelicans. Location of retrieval and total number of birds collected were documented. In all three years, the majority of botulism-affected pelicans were retrieved from the vicinities of the river deltas although the Whitewater River had the fewest birds of the three. It has yet to be determined what roles the tilapia and pelican populations play in influencing these patterns.

California↗

Ranking terrestrial vertebrate species for utility in biomonitoring and vulnerability to environmental contaminants

The measurement of contaminant tissue concentrations or exposure-related effects in biota has been used extensively to monitor pollution and environmental health. Terrestrial vertebrates have historically been an important group of species in such evaluations, not only because many are excellent sentinels of environmental contamination, but also because they are valued natural resources in their own right that may be adversely affected by toxicant exposure. Selection of appropriate vertebrates for biomonitoring studies frequently relies on expert opinion, although a few rigorous schemes are in use for predicting vulnerability of birds to the adverse effects of petroleum crude oil. A Utility Index that ranks terrestrial vertebrate species as potential sentinels of contaminants in a region, and a Vulnerability Index that assesses the threat of specific groups of contaminants to these species , have been developed to assist decision makers in risk assessments of persistent organic pollutants, cholinesterase-inhibiting pesticides, petroleum crude oil, mercury, and lead shot. Twenty-five terrestrial vertebrate species commonly found in Atlantic Coast estuarine habitat (Rattner et al. 2001a) were ranked for their utility as biomonitors of contamination and their vulnerability to pollutants in this region. No single species , taxa, or class of vertebrates was found to be an ideal sentinel for all groups of contaminants . Although birds have overwhelmingly been used to monitor contaminants compared to other terrestrial vertebrate classes, the nonmigratory nature and dietary habits of the snapping turtle and mink consistently resulted in ranking these species as excellent sentinels as well. Vulnerability of Atlantic Coast populations of these species varied considerably among groups of contaminants . Usually a particular species was found to be at high risk to only one or two groups of contaminants , although a noteworthy exception is the bald eagle, which is highly vulnerable to all five of the contaminant groups examined. This index could be further enhanced by generation of additional comparative toxicity data to facilitate interspecific extrapolations. The Utility and Vulnerability Indices have application to many types of habitats in addition to estuaries and are of value to natural resource and risk managers that routinely conduct local, regional, or national environmental quality assessments.

Archives of Environmental Contamination and Toxico↗

Long-term trends of local bird populations based on monitoring schemes: Are they suitable for justifying management measures?

Local biodiversity monitoring is important to assess the effects of global change, but also to evaluate the performance of landscape and wildlife protection, since large-scale assessments may buffer local fluctuations, rare species tend to be underrepresented, and management actions are usually implemented on local scales. We estimated population trends of 58 bird species using open-population N-mixture models based on count data in two localities in southeastern Spain, which have been collected according to a citizen science monitoring program (SACRE, Monitoring Common Breeding Birds in Spain) over 21 and 15 years, respectively. We performed different abundance models for each species and study area, accounting for imperfect detection of individuals in replicated counts. After selecting the best models for each species and study area, empirical Bayes methods were used for estimating abundances, which allowed us to calculate population growth rates ( λ ) and finally population trends. We also compared the two local population trends and related them with national and European trends, and species functional traits (phenological status, dietary, and habitat specialization characteristics). Our results showed increasing trends for most species, but a weak correlation between populations of the same species from both study areas. In general, local population trends were consistent with the trends observed at national and continental scales, although contrasting patterns exist for several species, mainly with increasing local trends and decreasing Spanish and European trends. Moreover, we found no evidence of a relationship between population trends and species traits. We conclude that using open-population N-mixture models is an appropriate method to estimate population trends, and that citizen science-based monitoring schemes can be a source of data for such analyses. This modeling approach can help managers to assess the effectiveness of their actions at the local level in the context of global change.

Journal of Ornithology↗

Salmonella enterica serovar Typhimurium from wild birds in the United States represent distinct lineages defined by bird type

Salmonella enterica serovar Typhimurium is typically considered a host generalist; however, certain isolates are associated with specific hosts and show genetic features of host adaptation. Here, we sequenced 131 S. Typhimurium isolates from wild birds collected in 30 U.S. states during 1978-2019. We found that isolates from broad taxonomic host groups including passerine birds, water birds (Aequornithes), and larids (gulls and terns) represented three distinct lineages and certain S. Typhimurium CRISPR types presented in individual lineages. We also showed that lineages formed by wild bird isolates differed from most isolates originating from domestic animal sources, and genomes from these lineages substantially improved source attribution of Typhimurium genomes to wild birds by a machine learning classifier. Furthermore, virulence gene signatures that differentiated S. Typhimurium from passerines, water birds, and larids were detected. Passerine isolates tended to lack S. Typhimurium-specific virulence plasmids. Isolates from the passerine, water bird, and larid lineages had close genetic relatedness with human clinical isolates, including those from a 2021 U.S. outbreak linked to passerine birds. These observations indicate that S. Typhimurium from wild birds in the United States are likely host-adapted, and the representative genomic dataset examined in this study can improve source prediction and facilitate outbreak investigation.

Applied and Environmental Microbiology↗

Demographic and spatiotemporal patterns of avian influenza infection at the continental scale, and in relation to annual life cycle of a migratory host

Since the spread of highly pathogenic avian influenza (HPAI) H5N1 in the eastern hemisphere, numerous surveillance programs and studies have been undertaken to detect the occurrence, distribution, or spread of avian influenza viruses (AIV) in wild bird populations worldwide. To identify demographic determinants and spatiotemporal patterns of AIV infection in long distance migratory waterfowl in North America, we fitted generalized linear models with binominal distribution to analyze results from 13,574 blue-winged teal ( Anas discors , BWTE) sampled in 2007 to 2010 year round during AIV surveillance programs in Canada and the United States. Our analyses revealed that during late summer staging (July-August) and fall migration (September-October), hatch year (HY) birds were more likely to be infected than after hatch year (AHY) birds, however there was no difference between age categories for the remainder of the year (winter, spring migration, and breeding period), likely due to maturing immune systems and newly acquired immunity of HY birds. Probability of infection increased non-linearly with latitude, and was highest in late summer prior to fall migration when densities of birds and the proportion of susceptible HY birds in the population are highest. Birds in the Central and Mississippi flyways were more likely to be infected compared to those in the Atlantic flyway. Seasonal cycles and spatial variation of AIV infection were largely driven by the dynamics of AIV infection in HY birds, which had more prominent cycles and spatial variation in infection compared to AHY birds. Our results demonstrate demographic as well as seasonal, latitudinal and flyway trends across Canada and the US, while illustrating the importance of migratory host life cycle and age in driving cyclical patterns of prevalence.

PLoS ONE↗

Evidence for foraging -site fidelity and individual foraging behavior of pelagic cormorants rearing chicks in the Gulf of Alaska

The Pelagic Cormorant ( Phalacrocorax pelagicus ) is the most widespread cormorant in the North Pacific, but little is known about its foraging and diving behavior. However, knowledge of seabirds' foraging behavior is important to understanding their function in the marine environment. In 2006, using GPS dataloggers, we studied the foraging behavior of 14 male Pelagic Cormorants rearing chicks on Middleton Island, Alaska. For foraging, the birds had high fidelity to a small area 8 km north of the colony. Within that area, the cormorants' diving activity was of two distinct kinds—near-surface dives (1–6 m) and benthic dives (28–33 m). Individuals were consistent in the depths of their dives, either mostly shallow or mostly deep. Few showed no depth preference. Dive duration, time at maximum depth, and pauses at the water surface between consecutive dives were shorter for shallow dives than for deep dives. The cormorants made dives of both types throughout the day, but the frequency of deep dives increased toward evening. Maximum foraging range was 9 km; maximum total distance traveled per trip was 43.4 km. Trip durations ranged from 0.3 to 7.7 hr. Maximum depth of a dive was 42.2 m, and duration of dives ranged from 4 to 120 sec. We found that Pelagic Cormorants at Middleton Island were faithful to one particular foraging area and individuals dived in distinct patterns. Distinct, specialized foraging behavior may be advantageous in reducing intra- and interspecific competition but may also render the species vulnerable to changing environmental conditions.

Alaska↗

Insular and migrant species, longevity records, and new species records on Guana Island, British Virgin Islands

We conducted mist netting each October from 1994 to 2004 on Guana Island, British Virgin Islands, and recorded bird sightings to develop a more complete inventory of the island's resident and migrant species. During our study, we recorded four new species for the British Virgin Islands: Magnolia Warbler (Dendroica magnolia; 1996), Golden-winged Warbler (Vermivora chrysoptera; 1997), Swainson's Thrush (Catharus ustulatus; 2000), and Red-necked Phalarope (Phalaropus lobatus; 2004). Blackpoll Warbler (Dendroica striata) was the most frequently captured Neotropical migrant landbird, despite only being first detected in the region in 1989. Captures and detections of other Neotropical migrant landbirds suggest that many species may be more common in the region than previously believed, or, as speculated by other researchers, that migrant routes may be shifting eastward due to habitat degradation on western Caribbean islands. We also used recapture data to establish longevity records of resident species, including Caribbean Elaenia (Elaenia martinica; ??? 7 years), Bananaquit (Coereba flaveola; 7 years), Black-faced Grassquit (Tiaris bicolor; ???9 years), and Zenaida Dove (Zenaida aurita; 5 years). Longevities of other resident species were similar to, or slightly less than, those reported elsewhere.

Wilson Journal of Ornithology↗

Occupancy in continuous habitat

The probability that a site has at least one individual of a species ('occupancy') has come to be widely used as a state variable for animal population monitoring. The available statistical theory for estimation when detection is imperfect applies particularly to habitat patches or islands, although it is also used for arbitrary plots in continuous habitat. The probability that such a plot is occupied depends on plot size and home-range characteristics (size, shape and dispersion) as well as population density. Plot size is critical to the definition of occupancy as a state variable, but clear advice on plot size is missing from the literature on the design of occupancy studies. We describe models for the effects of varying plot size and home-range size on expected occupancy. Temporal, spatial, and species variation in average home-range size is to be expected, but information on home ranges is difficult to retrieve from species presence/absence data collected in occupancy studies. The effect of variable home-range size is negligible when plots are very large (>100 x area of home range), but large plots pose practical problems. At the other extreme, sampling of 'point' plots with cameras or other passive detectors allows the true 'proportion of area occupied' to be estimated. However, this measure equally reflects home-range size and density, and is of doubtful value for population monitoring or cross-species comparisons. Plot size is ill-defined and variable in occupancy studies that detect animals at unknown distances, the commonest example being unlimited-radius point counts of song birds. We also find that plot size is ill-defined in recent treatments of "multi-scale" occupancy; the respective scales are better interpreted as temporal (instantaneous and asymptotic) rather than spatial. Occupancy is an inadequate metric for population monitoring when it is confounded with home-range size or detection distance.

Ecosphere↗

Common tern colonies along the mid-Atlantic coast. I. Nestling chronology

1. Sixteen Common Tern colonies in Rhode Island, New Jersey, Virginia, and North Carolina were studied from May through July 1980 by four investigators. Nests were marked during egg laying and were monitored every 5-7 days until fledging of young. 2. Results from 1980 indicate that ambient temperature differences in the four study areas account for differences in the onset of egg laying with North Carolina and Virginia colonies beginning 12-16 days before New Jersey and Rhode Island birds. Temperature alone, however, does not seem to have a significant effect on either median egg-laying dates or total duration of the season. 3. The low frequency of late-season nesting was not correlated with colony size or success of peak nests in 1980, but renesting (or late nesting) in all study areas was rare because of the benign weather conditions during the spring. Similarly, no significant correlation was found between success of nests and colony size.

Colonial Waterbirds↗

Preliminary Surficial Geologic Map of the Mesquite Lake 30' X 60' Quadrangle, California and Nevada

The Quaternary surficial geologic map of the Mesquite Lake, California-Nevada 30'X60' quadrangle depicts deposit age and geomorphic processes of erosion and deposition, as identified by a composite of remote sensing investigations, laboratory analyses, and field work, in the arid to semi-arid Mojave Desert area, straddling the California-Nevada border. Mapping was motivated by the need to address pressing scientific and social issues such as understanding and predicting the effects of climate and associated hydrologic changes, human impacts on landscapes, ecosystem function, and natural hazards at a regional scale. As the map area lies just to the south of Las Vegas, Nevada, a rapidly expanding urban center, land use pressures and the need for additional construction materials are forecasted for the region. The map contains information on the temporal and spatial patterns of surface processes and hazards that can be used to model specific landscape applications. Key features of the geologic map include: (1) spatially extensive Holocene alluvial deposits that compose the bulk of Quaternary units (~25%), (2) remote sensing and field studies that identified fault scarps or queried faults in the Kingston Wash area, Shadow Mountains, southern Pahrump Valley, Bird Spring Range, Lucy Gray Mountains and Piute Valley, (3) a lineament indicative of potential fault offset is located in Mesquite Valley, (4) active eolian dunes and sand ramps located on the east side of Mesquite, Ivanpah, and Hidden Valleys adjacent to playas, (4) groundwater discharge deposits in southern Pahrump Valley, Spring Mountains, and Lucy Gray Mountains and (5) debris-flow deposits spanning almost the entire Quaternary period in age.

Open-File Report↗

Food habits of American Kestrels in the Southern High Plains of Texas

The American Kestrel ( Falco sparverius ) is in general decline across its North American distribution. In contrast to widespread patterns of decline, kestrel populations appear stable in the southern Great Plains region. Historically, this region had a very low occurrence of kestrels, and their current abundance is highly likely due to vegetation and structures associated with settlement by people of European descent. To determine prey use by breeding kestrels, we placed motion-activated video cameras at preexisting kestrel nest boxes located in the Southern High Plains in 2017. We recorded over 4200 prey deliveries during 1748 hr of observation at five nests over the 4-wk brood-rearing period. On basis of frequency, these deliveries were dominated by reptiles (74.8%), with invertebrates (18.2%), mammals (4.4%), birds (2.9%), and unidentified (1.2%) prey used to lesser extents. Prey delivery rates were high relative to other studies; across the brood-rearing period we recorded an average of 2.3 deliveries/hr, equating to an average of 0.49 deliveries and 3.85 g of prey/nestling/hr. Because invertebrates dominate the diet reported in most kestrel food habit studies, the volume of reptiles captured as prey was unexpected. Even more unanticipated was the number of large prey captured, including juvenile eastern cottontails ( Sylvilagus floridanus ) and ground squirrels ( Ictidomys tridecemlineatus , Xerospermophilus spilosoma ). We suspect the proportion of vertebrate prey captured during the nesting season may explain the local high rates of nesting success and number of young fledged.

Texas↗

Trichomoniasis as a factor in mourning dove population decline in Fillmore, Utah

We examined whether trichomoniasis had been a factor in a dramatic mourning dove ( Zenaida macroura ) population decline in Fillmore, Utah (USA). We reasoned that if we could not find a high proportion of doves showing clinical signs of disease then the population was not being affected. Prevalences of Trichomonas gallinae in doves were 21% for 1992 and 14% for 1993. We also examined 230 birds and found only one with oral lesions. These prevalences were similar to those observed at the same study site in the 1950's, or reported elsewhere. Based on our results, we believe that trichomoniasis was not affecting the Fillmore mourning dove population at the time of our study.

Utah↗

Monitoring breeding and migration of neotropical migratory birds at Point Loma, San Diego County, California, 5-year summary, 2011–15

Executive Summary We operated a bird banding station on the Point Loma peninsula in western San Diego County, California, during spring and summer from 2011 to 2015. The station was established in 2010 as part of a long-term monitoring program for neotropical migratory birds during spring migration and for breeding birds as part of the Monitoring Avian Productivity and Survivorship (MAPS) program. During spring migration (April and May), 2011–15, we captured 1,760 individual birds of 54 species, 91 percent (1,595) of which were newly banded, fewer than 1 percent (3) of which were recaptures that were banded in previous years, and 9 percent (143 hummingbirds, 2 hawks, and 17 other birds) of which we released unbanded. We observed an additional 22 species that were not captured. Thirty-four individuals were captured more than once. Bird capture rate averaged 0.49 ± 0.07 captures per net-hour (range 0.41–0.56). Species richness per day averaged 6.87 ± 0.33. Cardellina pusilla (Wilson’s warbler) was the most abundant spring migrant captured, followed by Empidonax difficilis (Pacific-slope flycatcher), Vireo gilvus (warbling vireo), Zonotrichia leucophrys (white-crowned sparrow), and Selasphorus rufus (rufous hummingbird). Captures of white-crowned sparrow decreased, and captures of Pacific-slope flycatcher increased, over the 5 years of our study. Fifty-six percent of known-sex individuals were male and 44 percent were female. The peak number of new species arriving per day ranged from April 1 (2013-six species) to April 16 (2012-five species). A significant correlation was determined between the number of migrants captured each day per net-hour and the density of echoes on the Next-Generation Radar (NEXRAD) images across all 5 years, and in each year except 2014. NEXRAD radar imagery appears to be a useful tool for detecting pulses in migration. Our results indicate that Point Loma provides stopover habitat during migration for 76 migratory species, including 20 species of conservation concern. Two of these species, Vireo bellii pusillus (least Bell’s vireo) and Empidonax traillii (willow flycatcher) are listed as State and (or) federally threatened or endangered. Except for Archilochus alexandri (black-chinned hummingbird) and Setophaga occidentalis (hermit warbler), which arrived later during the migratory season in latter years of our study, median arrival dates for migratory species tended to be earlier each year or did not change across 5 years. Of the five most common migratory species, white-crowned sparrow and rufous hummingbird arrived earlier in latter years of the study, but Pacific-slope flycatcher, warbling vireo, and Wilson’s warbler median arrival dates were variable and showed no trend. We captured 1,680 individuals of 66 species during the MAPS/breeding season (May through August) across the 5 years of our study, 72 percent (1,211) of which were newly banded, 10 percent (167) of which were recaptures, and 18 percent (302 hummingbirds and other birds that escaped prior to banding) of which we released unbanded. Bird capture rate averaged 0.65 ± 0.21 captures per net-hour (range 0.12–2.54). Species richness per day ranged from 9.80 ± 5.01 to 14.20 ± 4.57. Calypte anna (Anna’s hummingbird) was the most abundant breeding species captured, followed by Oreothlypis celata (orange-crowned warbler), Psaltriparus minimus (bushtit), Pipilo maculatus (spotted towhee), Thryomanes bewickii (Bewick’s wren), Melozone crissalis (California towhee), and Chamaea fasciata (wrentit). Fifty-one percent of known-sex captures were female, and 49 percent were male. Thirty-one percent of known-age captures were juveniles. Populations of bushtits and orange-crowned warbler decreased significantly over 5 years. Anna’s hummingbird abundance was high for 4 years, and then decreased in 2015. Bewick’s wren and wrentit populations were highest in 2015. There was no obvious pattern in spotted towhee and California towhee abundance across 5 years. Annual breeding productivity for most species was low in 2014 and high in 2015. Bewick’s wren had the highest breeding productivity of the six most commonly captured species, followed by bushtit. Orange-crowned warbler had the lowest breeding productivity. Breeding productivity was a significant predictor of population size the next year for bushtit, but not for any other resident breeding species examined. Adult survivorship was generally high from 2013 to 14, and low from 2014 to 15. Wrentits had the highest survivorship of the most common species captured, followed by California towhee and orange-crowned warbler. Adult survivorship was lowest for bushtits and spotted towhees. Adult survivorship was a significant predictor of population size for bushtits, but not for any other resident species examined. Our monitoring results indicate that Point Loma provides breeding habitat for seven species of conservation concern. One of these species, the federally threatened Polioptila californica californica (California gnatcatcher), was documented breeding at the study site.

California↗

Woodcock utilization of commercial timberlands in the northeast

This paper reports the results of studies in Maine (1975-77) and New Brunswick (1974) on the utilization of commercial timber areas by woodcock (Philohela minor). Openings created by logging operations were utilized for singing grounds and nocturnal roosting habitat. Singing male densities of 3.4 birds/1oo ha on were found on New Brunswick elearcuts, 0.24 birds/IOO ha on northern Maine elearcuts, and 1.8-2.1 birds/IOO ha on central Maine selective cuts. Singing male densities in New Brunswick were greater on cuts smaller than 20 ha than on larger cuts. Compared to abandoned agricultural land and alder thickets, earthworm biomass on timber harvest areas was lower and fewer birds used the cuts as diurnal habitat. Regenerating stands provided low quality diurnal habitat, which appeared to limit woodcock numbers on the study areas. The importance of commercial timber harvest operations to future woodcock populations is discussed.

Book chapter↗

Detection of Splendidofilaria sp. (Onchocercidae:Splendidofilariinae) Microfilaria within Alaskan ground-dwelling birds in the grouse subfamily tetraoninae using taqman probe-based real-time PCR

Grouse and ptarmigan (Galliformes) harbor fairly diverse helminth faunas that can impact the host's health, including filarial nematodes in the genus Splendidofilaria . As host and parasite distributions are predicted to shift in response to recent climate change, novel parasites may be introduced into a region and impose additional stressors on bird populations. Limited information is available on the prevalence of filariasis in Alaska galliforms. To date, no molecular surveys have been completed. Past studies relied on examining blood smears or total body necropsies, which are time-consuming and may not detect filarial parasites with low prevalence in hosts. Therefore, we developed a TaqMan probe-based real-time PCR assay targeting the cytochrome c oxidase 1 gene ( COI ) of Splendidofilaria to decrease processing times and increase sensitivity as well as provide baseline data on the diversity of filariid infections in galliform species in Alaska. We screened a combined total of 708 galliform samples (678 unique individual birds) from different tissues (blood, muscle, and lung) for the presence of filarial DNA across the state of Alaska. Real-time PCR screening revealed an overall prevalence of filarial infection of 9.5% across species: Bonasa umbellus (0%, n = 23), Dendragapus fuliginosus (0%, n = 8), Falcipennis canadensis (26.8%, n = 198), Lagopus lagopus (2.6%, n = 274), Lagopus leucura (0%, n = 23), Lagopus muta (3%, n = 166), and Tympanuchus phasianellus (12.5%, n = 16). We observed microfilarial infections throughout most of Alaska except in Arctic regions and the Aleutian Islands where viable vectors may not be present.

Journal of Parasitology↗

Copper pellets simulating oral exposure to copper ammunition: Absence of toxicity in American kestrels ( Falco sparverius )

To evaluate the potential toxicity of copper (Cu) in raptors that may consume Cu bullets, shotgun pellets containing Cu, or Cu fragments as they feed on wildlife carcasses, we studied the effects of metallic Cu exposure in a surrogate, the American kestrel ( Falco sparverius ). Sixteen kestrels were orally administered 5 mg Cu/g body mass in the form of Cu pellets (1.18–2.00 mm in diameter) nine times during 38 days and 10 controls were sham gavaged on the same schedule. With one exception, all birds retained the pellets for at least 1 h, but most (69%) regurgitated pellets during a 12-h monitoring period. Hepatic Cu concentrations were greater in kestrels administered Cu than in controls, but there was no difference in Cu concentrations in the blood between treated and control birds. Concentration of the metal-binding protein metallothionein was greater in male birds that received Cu than in controls, whereas concentrations in female birds that received Cu were similar to control female birds. Hepatic Cu and metallothionein concentrations in kestrels were significantly correlated. Histopathologic alterations were noted in the pancreas of four treated kestrels and two controls, but these changes were not associated with hepatic or renal Cu concentrations, and no lesions were seen in other tissues. No clinical signs were observed, and there was no treatment effect on body mass; concentrations of Cu, hemoglobin, or methemoglobin in the blood; or Cu concentrations in kidney, plasma biochemistries, or hematocrit. Based on the parameters we measured, ingested Cu pellets pose little threat to American kestrels (and presumably phylogenetically related species), although the retention time of pellets in the stomach was of relatively short duration. Birds expected to regurgitate Cu fragments with a frequency similar to kestrels are not likely to be adversely affected by Cu ingestion, but the results of our study do not completely rule out the potential for toxicity in species that might retain Cu fragments for a longer time.

Archives of Environmental Contamination and Toxico↗

Ground water in the southeastern Uinta Basin, Utah and Colorado

The potential for developing oil-shale resources in the southeastern Uinta Basin of Utah and Colorado has created the need for information on the quantity and quality of water available in the area. This report describes the availability and chemical quality of ground water, which might provide a source or supplement of water supply for an oil-shale industry. Ground water in the southeastern Uinta Basin occurs in three major aquifers. Alluvial aquifers of small areal extent are present i n val ley-f i 11 deposits of six major drainages. Consolidated-rock aquifers include the birds's-nest aquifer i n the Parachute Creek Member of the G reen River Formation, which is limited to the central part of the study area; and the Douglas Creek aquifer, which includes parts of the Douglas Creek Member of the Green River Formation and parts of the intertonguing Renegade Tongue of the Wasatch Formation; this aquifer underlies most of the study area. The alluvial aquifers are recharged by infiltration of streamflow and leakage from consolidated-rock aquifers. Recharge is estimated to average about 32,000 acre-feet per year. Discharge from alluvial aquifers, primarily by evapotranspiration, also averages about 32,000 acre-feet per year. The estimated volume of recoverable water in storage in alluvial aquifers is about 200,000 acre-feet. Maximum yields to individual wells are less than 1,000 gallons per minute. Recharge to the bird's-nest aquifer, primarily from stream infiltration and downward leakage from the overlying Uinta Formation, is estimated to average 670 acre-feet per year. Discharge from the bird's-nest aquifer, which is primarily by seepage to Bitter Creek and the White River, is estimated to be at 670 acre-feet per year. The estimated volume of recoverable water in storage in the bird's-nest aquifer is 1.9 million acre-feet. Maximum yields to individual wells in some areas may be as much as 5,000 gallons per minute. A digital-computer model of the flow system was used to evaluate the effects of oil-shale development on the bird's-nest aquifer at the Federal lease tracts Ua and Ub. Results of model simulations indicate that during construction of a vertical access shaft, a pumping rate of about 900 gallons per minute would be required to dewaterthe aquifer. The model also indicates that the construction of a proposed reservoir on the White River may raise water levels in the bird's-nest aquifer near the reservoir site by as much as 45 feet. The flow model was used to evaluate the potential ground-water supply available for oil-shale development in the vicinity of the Federal lease tracts Ua and Ub. The results of the simulation indicate that bird's-nest aquifer could supply about 10,000 acre-feet of water per year at that site, for a period of 20 years. Downdraw after 20 years of pumping would exceed 250 feet near the simulated well field. Based on the results of the model simulation, it is estimated that the aquifer could simultaneously supply another 10,000 acre-feet of water per year in the northern part of the study area, but some interference between well fields could be expected. The Douglas Creek aquifer is recharged by precipitation and stream infiltration at an average rate of about 20.000 acre-feet per year. Discharge is estimated to be about the same and is primarily through springs and diffuse seepage. The estimated volume of recoverable water in storage is 16 million acre-feet. Maximum yields to individual wells are estimated to be less than 500 gallons per minute. A model of the flow system in the Douglas Creek aquifer indicates that the aquifer could supply about 700 acre-feet of water per year for oil-shale development at Federal lease tracts Ua and Ub and at the TOSCO Corp. site. After 20 years of pumping, water levels in production wells would be near the base of the aquifer. Based on the results of the model simulation, it is estimated that the aquifer could supply another 700 acre-feet of water per year in the southern part of the modeled area, but some interference between wells could be expected. Chemical quality of the ground water in the southeastern Uinta Basin varies considerably. Water from alluvial wells ranges from about 440 to 27,800 milligrams per liter of dissolved solids. Water from two consolidated-rock aquifers has dissolved-solids concentrations ranging from 870 to 5,810 milligrams per liter in the bird's-nest aquifer, and from 640 to 6,100 milligrams per liter in the Douglas Creek aquifer. Water from alluvial wells generally is a sodium sulfate type, whereas water in both the consolidated-rock aquifers generally changes from a sodium sulfate type to a sodium bicarbonate type. All ground water is very alkaline, and the alluvial aquifers contain very hard water. None of the water is suitable for public supply, but all the water could be used for industrial purposes such as washing and cooling. Changes in chemical composition of the ground water can be attributed to several physiochemical processes, including mineral precipitation and dissolution, oxidation and reduction, mixing, ion exchange, and evaporative concentration. Mass-transfer modeling of these processes shows how they can account for the variability in the ground-water quality. The mass-transfer model of the Bitter Creek alluvial aquifer shows that evaporative concentration, combined with precipitation of calcite, dolomite, gypsum, and release of carbon dioxide to the atmosphere results in the documented changes in the pH and dissolved solids in the water. The water-quality changes in the consolidated-rock aquifers are a result of precipitation of calcium carbonate and perhaps dolomite (calcium magnesium carbonate) with the reduction of sulfate by organic carbon, as well as ion exchange of magnesium for sodium. These processes result in large values of pH and alkalinity in the water.

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