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Ocean-atmosphere forcing of centennial hydroclimatic variability in the Pacific Northwest

Reconstructing centennial timescale hydroclimate variability during the late Holocene is critically important for understanding large-scale patterns of drought and their relationship with climate dynamics. We present sediment oxygen isotope records spanning the last two millennia from 10 lakes, as well as climate model simulations, indicating that the Little Ice Age was dry relative to the Medieval Climate Anomaly in much of the Pacific Northwest of North America. This pattern is consistent with observed associations between the El Niño Southern Oscillation (ENSO), the Northern Annular Mode and drought as well as with proxy-based reconstructions of Pacific ocean-atmosphere variations over the past 1000 years. The large amplitude of centennial variability indicated by the lake data suggests that regional hydroclimate is characterized by longer-term shifts in ENSO-like dynamics, and that an improved understanding of the centennial timescale relationship between external forcing and drought conditions is necessary for projecting future hydroclimatic conditions in western North America.

Pacific Northwest↗

Movements of four native Hawaiian birds across a naturally fragmented landscape

Animals often increase their fitness by moving across space in response to temporal variation in habitat quality and resource availability, and as a result of intra and inter-specific interactions. The long-term persistence of populations and even whole species depends on the collective patterns of individual movements, yet animal movements have been poorly studied at the landscape level. We quantified movement behavior within four native species of Hawaiian forest birds in a complex lava-fragmented landscape: Hawai‛i ‘amakihi Chlorodrepanis virens , ‘oma‘o Myadestes obscurus , ‘apapane Himatione sanguinea , and ‘i‘iwi Drepanis coccinea . We evaluated the relative importance of six potential intrinsic and extrinsic drivers of movement behavior and patch fidelity: 1) forest fragment size, 2) the presence or absence of invasive rats ( Rattus sp.), 3) season, 4) species, 5) age, and 6) sex. The study was conducted across a landscape of 34 forest fragments varying in size from 0.07 to 12.37 ha, of which 16 had rats removed using a treatment-control design. We found the largest movements in the nectivorous ‘apapane and ‘i‘iwi, intermediate levels in the generalist Hawai‛i ‘amakihi, and shortest average movement for the ‘oma‘o, a frugivore. We found evidence for larger patch sizes increasing patch fidelity only in the ‘oma‘o, and an effect of rat-removal increasing patch fidelity of Hawai‛i ‘amakihi only after two years of rat-removal. Greater movement during the non-breeding season was observed in all species, and season was an important factor in explaining higher patch fidelity in the breeding season for ‘apapane and ‘i‘iwi. Sex was important in explaining patch fidelity in ‘oma‘o only, with males showing higher patch fidelity. Our results provide new insights into how these native Hawaiian species will respond to a changing environment, including habitat fragmentation and changing distribution of threats from climate change.

Journal of Avian Biology↗

Relationship between oxidative stress, pathology, and behavioral signs of lead poisoning in mallards

Some of the adverse effects of lead (Pb) may be associated with oxidative damage of lipids, proteins, or DNA. In a previous study a linkage was observed between the susceptibilities of waterfowl species to Pb poisoning with oxidative stress . To investigate this relationship among the individuals of a single species, for 3 wk 4 groups of 72 mallards were fed diets containing high or low levels of vitamin E (20 or 220 Ul/kg) and high or low levels of Pb (0 or 2 g/kg). During the first week of Pb exposure, mallards developed hemolytic anemia, and during the second week, signs of neurological impairment. Histological findings in the Pb-exposed mallards were hemosiderosis, demyelinization of sciatic and brachial nerves, and tumefaction of renal tubular epithelium with the presence of intranuclear inclusion bodies. Lipid peroxidation increased with Pb exposure in blood, liver, bile, and brain, but decreased in nerves. Glutathione (GSH) increased with Pb exposure in liver and bile, and its oxidized/reduced ratio only increased in bile. Pb exposure inhibited GSH peroxidase activity (GPX) in plasma, liver, and brain, and decreased protein thiols (PSH) in blood and liver. Vitamin E resulted in significantly lower lipid peroxidation in nerves of control birds relative to unsupplemented controls, but did not alleviate any sign of lead posioning. Pb-induced pathological changes associated with hepatic and nervous functions were significantly correlated with lower GPX activity and PSH concentrations in these tissues rather than lipid peroxidation. Data suggest that inhibition of antioxidant enzymes and interaction with sulfhydryl groups of proteins may play a more important role in Pb poisoning of waterfowl than lipid peroxidation.

Journal of Toxicology and Environmental Health, Pa↗

Evidence of Absence software

Evidence of Absence software (EoA) is a user-friendly application used for estimating bird and bat fatalities at wind farms and designing search protocols. The software is particularly useful in addressing whether the number of fatalities has exceeded a given threshold and what search parameters are needed to give assurance that thresholds were not exceeded. The software is applicable even when zero carcasses have been found in searches. Depending on the effectiveness of the searches, such an absence of evidence of mortality may or may not be strong evidence that few fatalities occurred. Under a search protocol in which carcasses are detected with nearly 100 percent certainty, finding zero carcasses would be convincing evidence that overall mortality rate was near zero. By contrast, with a less effective search protocol with low probability of detecting a carcass, finding zero carcasses does not rule out the possibility that large numbers of animals were killed but not detected in the searches. EoA uses information about the search process and scavenging rates to estimate detection probabilities to determine a maximum credible number of fatalities, even when zero or few carcasses are observed.

Data Series↗

Genetic structure of the Common Eider in the western Aleutian Islands prior to fox eradication

Since the late 18 th century bird populations residing in the Aleutian Archipelago have been greatly reduced by introduced arctic foxes ( Alopex lagopus ). We analyzed data from microsatellite, nuclear intron, and mitochondrial (mtDNA) loci to examine the spatial genetic structure, demography, and gene flow among four Aleutian Island populations of the Common Eider ( Somateria mollissima ) much reduced by introduced foxes. In mtDNA, we found high levels of genetic structure within and between island groups (Φ ST = 0.643), but we found no population subdivision in microsatellites or nuclear introns. Differences in genetic structure between the mitochondrial and nuclear genomes are consistent with the Common Eider's breeding and winter biology, as females are highly philopatric and males disperse. Nevertheless, significant differences between islands in the mtDNA of males and marginal significance ( P = 0.07) in the Z -linked locus Smo 1 suggest that males may also have some level of fidelity to island groups. Severe reduction of populations by the fox, coupled with females' high philopatry, may have left the genetic signature of a bottleneck effect, resulting in the high levels of genetic differentiation observed in mtDNA (Φ ST = 0.460–0.807) between islands only 440 km apart. Reestablishment of the Common Eider following the fox's eradication was likely through recruitment from within the islands and bolstered by dispersal from neighboring islands, as suggested by the lack of genetic structure and asymmetry in gene flow between Attu and the other Near Islands.

Aleutian Islands↗

A GIS modeling method applied to predicting forest songbird habitat

We have developed an approach for using “presence” data to construct habitat models. Presence data are those that indicate locations where the target organism is observed to occur, but that cannot be used to define locations where the organism does not occur. Surveys of highly mobile vertebrates often yield these kinds of data. Models developed through our approach yield predictions of the amount and the spatial distribution of good-quality habitat for the target species. This approach was developed primarily for use in a GIS context; thus, the models are spatially explicit and have the potential to be applied over large areas. Our method consists of two primary steps. In the first step, we identify an optimal range of values for each habitat variable to be used as a predictor in the model. To find these ranges, we employ the concept of maximizing the difference between cumulative distribution functions of (1) the values of a habitat variable at the observed presence locations of the target organism, and (2) the values of that habitat variable for all locations across a study area. In the second step, multivariate models of good habitat are constructed by combining these ranges of values, using the Boolean operators “and” and “or.” We use an approach similar to forward stepwise regression to select the best overall model. We demonstrate the use of this method by developing species-specific habitat models for nine forest-breeding songbirds (e.g., Cerulean Warbler, Scarlet Tanager, Wood Thrush) studied in southern Ohio. These models are based on species’ microhabitat preferences for moisture and vegetation characteristics that can be predicted primarily through the use of abiotic variables. We use slope, land surface morphology, land surface curvature, water flow accumulation downhill, and an integrated moisture index, in conjunction with a land-cover classification that identifies forest/nonforest, to develop these models. The performance of these models was evaluated with an independent data set. Our tests showed that the models performed better than random at identifying where the birds occurred and provided useful information for predicting the amount and spatial distribution of good habitat for the birds we studied. In addition, we generally found positive correlations between the amount of habitat, as predicted by the models, and the number of territories within a given area. This added component provides the possibility, ultimately, of being able to estimate population sizes. Our models represent useful tools for resource managers who are interested in assessing the impacts of alternative management plans that could alter or remove habitat for these birds.

Ecological Applications↗

Effects of flow modification on a cattail wetland at the mouth of Irondequoit Creek near Rochester, New York: Water levels, wetland biota, sediment, and water quality

An 11-year (1990-2001) study of the Ellison Park wetland, a 423-acre, predominantly cattail (Typha glauca) wetland at the mouth of Irondequoit Creek, was conducted to document the effects that flow modifications, including installation of a flow-control structure (FCS) in 1997 and increased diversion of stormflows to the backwater areas of the wetland, would have on the wetland's ability to decrease chemical loads transported by Irondequoit Creek into Irondequoit Bay on Lake Ontario. The FCS was designed to raise the water-surface elevation and thereby increase the dispersal and detention of stormflows in the upstream half of the wetland; this was expected to promote sedimentation and microbial utilization of nutrients, and thereby decrease the loads of certain constituents, primarily phosphorus, that would otherwise be carried into Irondequoit Bay. An ecological monitoring program was established to document changes in the wetland's water levels, biota, sedimentation rates, and chemical quality of water and sediment that might be attributable to the flow modifications. Water-level increases during storms were mostly confined to the wetland area, within about 5,000 ft upstream from the FCS. Backwater at a point of local concern, about 13,000 ft upstream, was due to local debris jams or constriction of flow by bridges and was not attributable to the FCS. Plant surveys documented species richness, concentrations of nutrients and metals in cattail tissues, and cattail productivity. Results indicated that observed differences among survey periods and between the areas upstream and downstream from the FCS were due to seasonal changes in water levels—either during the current year or at the end of the previous year's growing season—that reflected the water-surface elevation of Lake Ontario, rather than water-level control by the FCS. Results showed no adverse effects from the naturally high water levels that prevail annually during the spring and summer in the wetland, nor from the short-duration increases in water levels that result from FCS operation. Fish surveys documented the use of the wetland by 44 species, of which 25 to 29 species were found in any given year. Community composition was relatively consistent during the study, but seasonal and year-to-year variations in dominant resident and nonresident species were noted, and probably reflected natural or regional population patterns in Lake Ontario and Irondequoit Bay. The FCS allowed fish passage at all water levels and had no discernible adverse effect on the fish community. Bird surveys documented the use of the wetland by more than 90 species for breeding, feeding, and migration. Ground-nesting birds were unaffected by the FCS. Seasonally high water levels, rather than short-duration increases caused by the FCS, might have caused the scarcity or absence of certain wetland species by limiting the extent of breeding habitat for some species and the exposure of mud flats that attracted other species. Some noticeably scarce or absent species also were rare or absent elsewhere along the south-central shore of Lake Ontario. Benthic-macroinvertebrate studies were of minimal use for evaluating the effect of the FCS because no surveys were conducted after FCS installation. The precontrol results allowed assessment of the ecological quality of the wetland on the basis of biotic indices, and generally indicated moderately to severely impaired conditions. Differences between the macroinvertebrate communities in the southern part of the wetland and those in the northern part were attributed to habitat differences, such as substrate composition, water depth, and density of submerged aquatic vegetation. Sedimentation rates in the areas upstream and downstream from the FCS increased after the flow modifications, more in the area upstream from the FCS than in the downstream area. The concurrent downstream increase and the dynamic patterns of deposition and scour indicated that although the FCS and the other flow modifications undoubtedly were major factors in the postcontrol upstream increase in sedimentation rates, other factors, such as the magnitude, frequency, and the timing (season) of peak flows, might also have contributed. Periodic analyses of sediment samples from three longterm depositional sites in the wetland documented the concentrations of major and trace elements, polycyclic aromatic hydrocarbons, and organochlorine and organophosphate compounds. The concentrations of most constituents showed no substantial fluctuation or consistent upward or downward trend during the years sampled, nor did they identify any change after FCS installation. Comparison of the measured concentrations with sediment-quality guidelines that are used to assess the ecological quality of substrate environments indicated that the wetland was moderately to severely impaired—an assessment consistent with the benthic-macroinvertebrate biotic indices. During the precontrol period (1990–96), the wetland was a sink for particulate constituents (removal efficiencies for total phosphorus and total suspended solids were 28 and 47 percent, respectively), but had little effect on conservative constituents (chloride and sulfate). The wetland was a source of orthophosphate and ammonia (removal efficiencies were -38 and -84 percent, respectively). During the postcontrol period (1997–2001), the wetland continued to be a sink for particulate constituents (removal efficiencies for total phosphorus and total suspended solids were 45 and 52 percent, respectively); the exportation of orthophosphate by the wetland decreased (by 7 percent), whereas that of ammonia increased (by about 70 percent). The outflow loads of orthophosphate and ammonia represented about 15 and 2.3 percent of total phosphorus and total nitrogen loads, respectively. Changes in the loads of conservative constituents were negligible, and the overall removal efficiencies for other constituents during the precontrol period differed from those of the postcontrol period by no more than 5.4 percent. Statistical analyses of monthly inflow and outflow loads indicated significant differences between inflow and outflow loads of most constituents during the pre- and postcontrol periods. Load data were adjusted to remove the effects of dissimilar hydrologic conditions that prevailed during the pre- and postcontrol periods, and to isolate the water-quality-improvement effect that could be attributed solely to the FCS. Results indicated that the FCS contributed significantly to the decrease in total phosphorus loads, and slightly to a decrease in ammonia-plus-organic nitrogen loads, but had little or no significant effect on loads of other constituents.

New York↗

A genetic evaluation of morphology used to identify harvested Canada geese

Using maximum likelihood estimators (in genetic stock identification), we used genetic markers to evaluate the utility of 2 morphological measures (culmen length and plumage color) to correctly identify groups of hunter-harvested dusky (Branta canadensis occidentalis) and dusky-like Canada geese on the wintering grounds within the Pacific Flyway. Significant levels of genetic differentiation were observed across all sampled breeding sites for both nuclear microsatellite loci and mtDNA when analyzed at the sequence level. The ability to discriminate among geese from these sites using genetic markers was further demonstrated using computer simulations. We estimated contributions from the Copper River Delta, the primary breeding area of dusky Canada geese, to groups of hunter-harvested geese classified as dusky Canada geese on the basis of morphology as 50.6 ?? 10.1(SE)% for females and 50.3 ?? 13.0% for males. We also estimated that 16 ?? 8.1% of females classified as dusky Canada geese on the basis of morphology originated from Middleton Island, Alaska; a locale currently managed as a subpopulation of dusky Canada geese, even though the majority of geese from this area possess a unique mtdna haplotype not found on the Copper River Delta. The use of culmen length and plumage color to identify the origin of breeding populations in the harvest provides conservative criteria for management of dusky Canada geese as individuals of other breeding populations are misassigned as dusky Canada geese and birds of the lighter-plumaged dusky-like group did not appear to originate from, breeding sites of the dusky Canada goose. Our analyses demonstrate that genetic markers can accurately estimate the proportion of genetically differentiated areas that comprise an admixed group, but they also raise questions about the management scale of Pacific Flyway Canada geese (e.g., at the subspecies or breeding population level) and the use of morphological and genetic characteristics to monitor the harvest of different populations within admixed wintering flocks.

Journal of Wildlife Management↗

Dynamic habitat selection by two wading bird species with divergent foraging strategies in a seasonally fluctuating wetland

Seasonal and annual variation in food availability during the breeding season plays an influential role in the population dynamics of many avian species. In highly dynamic ecosystems like wetlands, finding and exploiting food resources requires a flexible behavioral response that may produce different population trends that vary with a species' foraging strategy. We quantified dynamic foraging-habitat selection by breeding and radiotagged White Ibises ( Eudocimus albus ) and Great Egrets ( Ardea alba ) in the Florida Everglades, where fluctuation in food resources is pronounced because of seasonal drying and flooding. The White Ibis is a tactile “searcher” species in population decline that specializes on highly concentrated prey, whereas the Great Egret, in a growing population, is a visual “exploiter” species that requires lower prey concentrations. In a year with high food availability, resource-selection functions for both species included variables that changed over multiannual time scales and were associated with increased prey production. In a year with low food availability, resource-selection functions included short-term variables that concentrated prey (e.g., water recession rates and reversals in drying pattern), which suggests an adaptive response to poor foraging conditions. In both years, the White Ibis was more restricted in its use of habitats than the Great Egret. Real-time species—habitat suitability models were developed to monitor and assess the daily availability and quality of spatially explicit habitat resources for both species. The models, evaluated through hindcasting using independent observations, demonstrated that habitat use of the more specialized White Ibis was more accurately predicted than that of the more generalist Great Egret.

The Auk↗

Emergent sandbar construction for least terns on the Missouri River: Effects on forage fishes in shallow-water habitats

Emergent sandbars on the Missouri River are actively managed for two listed bird species, piping plovers and interior least terns. As a plunge-diving piscivore, endangered least terns rely on ready access to appropriately sized slender-bodied fish: <52 mm total length for adults and <34 mm total length for young chicks. As part of a multi-agency recovery programme, aimed at enhancing nesting habitat for plovers and terns, the U.S. Army Corps of Engineers mechanically created several emergent sandbars on the Missouri River. However, it was unknown whether sandbar construction is a benefit or a detriment to forage abundance for least terns. Therefore, we studied the shallow-water (<1.5 m) fish community near river and mechanically created emergent sandbars during three nesting seasons (2006–2008). We sampled every 2 weeks each year from late May to July within 15–16 areas to document the relative abundance, species richness and size classes of fish. Fish relative abundance was negatively related to depth. Catches were dominated by schooling species, including emerald shiner, sand shiner, spotfin shiner and bigmouth buffalo. Significant inter-annual differences in relative abundance were observed, with generally increasing trends in intra-seasonal relative abundance of shiners and the smallest size classes of fish (<34 mm). Significant differences in the fish communities between the sandbar types were not detected in this study. Results suggest that mechanical sandbar habitats host comparable fish communities at similar levels of relative abundance. Further analyses are required to evaluate if the levels of fish relative abundance are adequate to support least tern foraging and reproduction.

Nebraska, South Dakota↗

Long-term prairie falcon population changes in relation to prey abundance, weather, land uses, and habitat conditions

We studied a nesting population of Prairie Falcons ( Falco mexicanus ) in the Snake River Birds of Prey National Conservation Area (NCA) from 1974-1997 to identify factors that influence abundance and reproduction. Our sampling period included two major droughts and associated crashes in Townsend's ground squirrel ( Spermophilus townsendii ) populations. The number of Prairie Falcon pairs found on long-term survey segments declined significantly from 1976-1997. Early declines were most severe at the eastern end of the NCA, where fires and agriculture have changed native shrubsteppe habitat. More recent declines occurred in the portion of canyon near the Orchard Training Area (OTA), where the Idaho Army National Guard conducts artillery firing and tank maneuvers. Overall Prairie Falcon reproductive rates were tied closely to annual indexes of ground squirrel abundance, but precipitation before and during the breeding season was related inversely to some measures of reproduction. Most reproductive parameters showed no significant trends over time, but during the 1990s, nesting success and productivity were lower in the stretch of canyon near the OTA than in adjacent areas. Extensive shrub loss, by itself, did not explain the pattern of declines in abundance and reproduction that we observed. Recent military training activities likely have interacted with fire and livestock grazing to create less than favorable foraging opportunities for Prairie Falcons in a large part of the NCA. To maintain Prairie Falcon populations in the NCA, managers should suppress wildfires, restore native plant communities, and regulate potentially incompatible land uses.

The Condor↗

Coast salish and U.S. Geological Survey: Tribal journey water quality project

The ancestral waters of the Coast Salish People, the Salish Sea, comprise a large inland sea contained within both United States (Puget Sound) and Canadian (Georgia Strait) territory. The Salish Sea is home to more than 220 species of fish, 29 species of marine mammals, more than 40 species of commercial and recreationally harvested invertebrates, and numerous resident and migratory bird species (Washington Sea Grant Program, 2000). Unfortunately, at least 60 of these marine based species are listed as threatened, endangered or of concern (Fraser and others, 2006), many of which sustained Coast Salish for millennia and are of essential cultural importance. The cumulative impacts of human activities and climate change are deteriorating coastal ecosystems and accelerating the loss of ecologically and culturally important marine resources. Watershed modifications, coastal development and industrial activities are altering river and tidal flow, sediment transport, and nutrient delivery all across the region, leading to the break down of ecosystem functions and decreasing biodiversity, thus changing the face of the Salish Sea. A cooperative trans-boundary partnership between the U.S. Environmental Protection Agency (USEPA) and the Government of Canada has identified Salish Sea indicators of health. Several of these indicators have been noted as having degrading quality including urbanization and forest change, river, stream, and lake quality, marine species at risk, toxics in harbor seals, and marine water quality conditions (USEPA, 2008). The functioning of the Salish Sea ecosystem is increasingly threatened by ever more frequent observations and expanding zones of anoxia. The complexities of monitoring, protecting, and restoring such a large and diverse geographical area are exacerbated by a political border. The Coast Salish Peoples and U.S. Geological Survey (USGS) have commenced on a partnership to examine water quality throughout the Georgia Straits and Puget Sound, blending tradition and science, in response to this deterioration of coastal environments and loss of essential habitats and marine resources of cultural and ecological importance throughout the ancestral waters of the Salish Sea. This report describes the Coast Salish Tribal Journey Water Quality Project, its inception, the results of the 2008 Tribal Journey project, lessons learned, and recommendations for future directions.

Report↗

Preening behavior of adult gyrfalcons tagged with backpack transmitters

Radio transmitters provide data that enhance understanding of raptor biology (Walls and Kenward 2007) and are now used to answer a multitude of research questions (Meyburg and Fuller 2007). However, transmitters affect the birds that carry them (Barron et al. 2010), and it is important to document and evaluate such effects (Casper 2009). For example, decreased survival has been documented in Prairie Falcons (Falco mexicanus; Steenhof et al. 2006), Northern Goshawks (Accipiter gentilis; Reynolds et al. 2004), and Spotted Owls (Strix occidentalis; Paton et al. 1991) tagged with radio transmitters. However, no such effects were reported for Peregrine Falcons (Falco peregrinus; Fuller et al. 1998, McGrady et al. 2002) and a number of other species (Kenward 2001). White and Garrott (1990) noted that in general, animals tagged with radio transmitters often altered their behaviors for 1–14 d after release during an adjustment period that included increased preening and grooming frequencies. Although more than 90 Gyrfalcons (Falco rusticolus) have been tagged with radio transmitters (e.g., Burnham 2007, McIntyre et al. 2009, T. Booms unpubl. data), the effects of transmitters on this species are not well documented. Anecdotal information suggests some Gyrfalcons might be negatively affected by radio-tagging (Booms et al. 2008). As part of a study investigating Gyrfalcon breeding biology, we conducted opportunistic, focused observations on two radio-tagged adult female Gyrfalcons and their unmarked mates. We here describe and quantify preening behavior of Gyrfalcons shortly after radio-tagging.

Journal of Raptor Research↗

FrogwatchUSA

full text: Frogs and toads are perhaps the most approachable and available of all our wildlife. In many, if not most places, they are abundant. In wetter parts of the East, almost anyone outside on a warm rainy night in spring will hear their dream-like calls, bellows, trills and snores. Even in the deserts of the Southwest, a nocturnal trip after a summer monsoon will yield toads moving across the roads toward a cacophonous orgy of mating and calling in the roadside ditches and desert pools. Birds share with frogs and toads this same sense of presence in our daily lives. But the difference is that birds are like the attractive neighbor who just never gives you the time of day, while frogs are more like the troglodyte who appears regularly to chat, philosophize, and have a beer. Uninvited, frogs appear in our water gardens, toads are on our stoops in the morning, we catch them when we are kids, raise their babies in the aquarium, and feel sorry when we find we have run them over with the lawnmower. When concerns about declining populations of amphibians reached the mass media, the Secretaries' office became involved. In addition to using traditional research mechanisms to investigate the problem, the Secretary also wanted to involve the public directly. The combination of high public appeal and the relative ease with which frog calls can be learned made a large-scale monitoring program for frogs and toads possible. What emerged was a program called Frogwatch USA, modeled after a successful Canadian program with a similar name. A web site was created (www.frogwatch.org) that presented potential frogwatchers with directions and a way to register their site online as well as enter their data. Observers chose where to count frogs depending on what they felt was important. For some it was their backyard, others chose vulnerable wetlands in their neighborhoods, or spots on local refuges and parks. Initially funded at $8,000 a year and then after two years increased to $25,000, most of the first part of this program's life was spent developing the tools and web site to document counts of frogs online. Despite the lack of time available to promote the program, send out press releases and recruit observers, news of the program quickly spread by word of mouth and the electronic media. Many newspaper articles later, we found a large number of people had become involved with counting frogs in their neighborhoods and backyards. Current figures show 1,456 observers who have registered at 1,683 sites logging almost 5,000 visits. These visits yield information on when and what species are calling from wetlands throughout the United States. These records are usually the only records of information about frogs and toads for those sites and become a permanent record that can be revisited in future years. Additionally, when observers make a lot of visits or there are many sites in a region, a phenology of calls can be created that documents when it is most likely in that year for each species to be recorded. Finally, even for those observers whose data we may mistrust and therefore are likely to eliminate from analyses, these people have taken the time to leave their televisions, go outside, and directly experience frogs, toads, and all that occur in Nature. In 1999 it was decided that FrogwatchUSA needed to work with another group that specifically focused on environmental education and outreach. After talking to a number of organizations we found that the National Wildlife Federation, with their Backyard Wildlife Habitat, Endangered Species, and other programs along with their four million members who are interested in nature, would be an excellent match. Thus a partnership was born. After over a year of work between Interior and National Wildlife Federation biologists and lawyers, an agreement has been created that places the Federation as the lead of Frogwatch USA. It will now take care of res

People, Land, and Water↗

A catalog of Louisiana's nesting seabird colonies

Summarizing his colonial nesting waterbird survey experiences along the northern coast of the Gulf of Mexico in a paper presented to the Colonial Waterbird Group of the Waterbird Society (Portnoy 1978), bird biologist John W. Portnoy stated, “This huge concentration of nesting waterbirds, restricted almost entirely to the wetlands and estuaries of southern Louisiana, is unmatched in all of North America; for example, a 1975 inventory of wading birds along the Atlantic Coast from Maine to Florida [Custer and Osborn, in press], tallied 250,000 breeding [waterbirds] of 14 species, in contrast with the 650,000 birds of 15 species just from Sabine Pass to Mobile Bay.” The “650,000 birds” to which Portnoy referred, were tallied by him in a 1976 survey of coastal Louisiana, Mississippi, and Alabama (see below, under “Major Surveys” section). According to the National Atlas of Coastal Waterbird Colonies in the Contiguous United States: 1976-82 (Spendelow and Patton 1988), the percentages of the total U.S. populations of Laughing Gull (11%), Forster's Tern (52%), Royal Tern (16%), Sandwich Tern (77%), and Black Skimmer (44%) which annually nest in Louisiana are significant – perhaps crucially so in the cases of Forster's Tern, Sandwich Tern, and Black Skimmer. Nearly three decades after Spendelow and Patton's determinations above, coastal Louisiana still stands out as the major center of colonial wading bird and seabird nesting in all of the United States. Within those three intervening decades, however, the collective habitats which comprise Louisiana's now fragile coastal zone have taken major hits from commercial/residential, oil & gas, and other industrial development, primarily in the form of coastal erosion exacerbated by these and other factors (Portnoy 1978, Spendelow and Patton 1988, Martin and Lester 1990, Green, et al. 2006). Moreover, during this same period, both geologic subsidence rates (Tornqvist et al. 2008) and mean sea-level (Tornqvist et al. 2002) have increased, along with significant tropical storm activity; all of which have combined to impact available marsh, barrier island, beach, and dredge spoil nesting habitat for waterbirds, especially seabirds, throughout the coastal zone of Louisiana. The primary objective of this publication is to detail those coastal Louisiana colonial seabird nesting sites for which we have reasonably accurate data, in a tabular, site-by-site format. All major survey (1976-2008) data of site-by-site seabird species counts, as well as several smaller data sets, referred to in the site history tables as “miscellaneous observations” obtained during the May-June seabird breeding period, are included. It is our hope that these data will provide a dependable foundation from which future colonial seabird nesting surveys might be planned and carried out, as well as showcase the importance of coastal Louisiana's seabird rookeries, and contribute to their conservation.

Louisiana↗

Hybridization and asymmetrical introgression between the vulnerable Gray‐Headed Chickadee and a more abundant congener, the Boreal Chickadee: Implications for conservation

Hybridization is a common process among bird species that can precipitate a mix of positive or negative species outcomes. Particularly for rare populations, detrimental effects of hybridization on demographic growth rates and genetic integrity are of serious concern. In Alaska and a small region of northwestern Canada, the endemic subspecies of Gray-headed Chickadee ( Poecile cinctus lathami ) has declined in recent decades from being locally common to being extremely rare. The more widespread Boreal Chickadee ( P. hudsonicus ) has become increasingly abundant in areas of sympatry. These changes in abundance may have led to hybridization between Gray-headed Chickadees and Boreal Chickadees. We used a series of analyses to test for signatures of introgression at mitochondrial DNA and nuclear DNA using historical museum samples of both species collected between 1875 and 1979 as well as contemporary Boreal Chickadee samples. In addition, we modeled Gray-headed Chickadee and Boreal Chickadee demographic histories to better understand patterns of effective population size changes and gene flow over time. Introgression of Gray-headed Chickadee nuclear DNA was detected in contemporary and historical Boreal Chickadee populations, and two first-generation hybrid backcrosses were observed in the historical Boreal Chickadee samples. Lack of mitochondrial DNA introgression or backcrossing into the Gray-headed Chickadee historical samples may be an artifact of mate scarcity during the period before local abundances of Boreal Chickadee exceeded Gray-headed Chickadees. Demographic modeling with nuclear loci estimated a low level of symmetric gene flow between Gray-headed Chickadees and Boreal Chickadees since the time of divergence. Our study suggests that hybridization may be linked to Gray-headed Chickadee declines and represents a case study of how museum collections can be used to infer introgression in a population too scarce to directly investigate.

Ecology and Evolution↗

Use of blood clotting assays to assess potential anticoagulant rodenticide exposure and effects in free-ranging birds of prey

Non-target wildlife, particularly birds of prey, are widely exposed to and acutely poisoned by anticoagulant rodenticides (ARs). An unresolved issue surrounding such exposure, however, is the potential for sublethal effects. In particular, the consequences of AR exposure and resulting coagulopathy on health and survival of unintentionally exposed animals, which often encounter a multitude of anthropogenic stressors, are understudied. In a wildlife rehabilitation setting, AR intoxication may be masked by more obvious injuries related to collision with vehicles or electrocution, thereby obfuscating proximate from ultimate cause of mortality. An assessment of coagulation function of admitted wildlife may provide a means of identifying animals exhibiting sublethal coagulopathy, and ultimately ensuring provision of appropriate and swift treatment. In conjunction with routine diagnostics for injury and disease, we performed two blood clotting assays (prothrombin time, Russell's viper venom time) affected by vitamin K-dependent coagulopathy of samples from six species of live raptors admitted to a rehabilitation facility. We also measured clotting time in pre-fledgling barn owl chicks ( Tyto furcata ) from 10 nest sites in Lower Mainland Canada. Prolonged clotting time or failure to form a clot altogether was observed in 23.0% of 61 sampled raptors admitted to the rehabilitation facility. This is a biologically significant proportion of individuals given the fortuitous and likely biased nature by which raptors are found and admitted to rehabilitation facilities. In contrast, there was little evidence of coagulopathy in 19 pre-fledgling barn owl chicks. The utility of avian coagulation tests for diagnosing AR exposure is promising, yet there remains a need to establish species specific reference values and standardize assay methodologies among testing facilities.

Science of the Total Environment↗

Geographic variation in the intensity of warming and phenological mismatch between Arctic shorebirds and invertebrates

Responses to climate change can vary across functional groups and trophic levels, leading to a temporal decoupling of trophic interactions or ‘phenological mismatches.’ Despite a growing number of single-species studies that identified phenological mismatches as a nearly universal consequence of climate change, we have a limited understanding of the spatial variation in the intensity of this phenomenon nor what influences this variation. In this study, we tested for geographic patterns in phenological mismatches between six species of shorebirds and their invertebrate prey at ten sites spread across ~13º latitude and ~84º longitude in the Arctic over three years. At each site, we quantified the phenological mismatch between shorebirds and their invertebrate prey at: 1) an individual nest level, as the difference in days between the seasonal peak in food and the peak demand by chicks, and 2) a population level, as the overlapped area under fitted curves for total daily biomass of invertebrates and dates of the peak demand by chicks. We tested whether the intensity of past climatic change observed at each site corresponded with the extent of phenological mismatch and used Structural Equation Modeling to test for causal relationships among: 1) environmental factors, including geographic location and current climatic conditions, 2) the timing of invertebrate emergence and the breeding phenology of shorebirds, and 3) the phenological mismatch between the two trophic levels. The extent of phenological mismatch varied more among different sites than among different species within each site. A greater extent of phenological mismatch at both the individual-nest and population-levels coincided with changes in the timing of snowmelt as well as the potential dissociation of long-term snow phenology from changes in temperature. The timing of snowmelt also affected the shape of the food and demand curves, which determined the extent of phenological mismatch at the population level. Finally, we found larger mismatches at more easterly longitudes, which may be affecting the population dynamics of shorebirds, as two of our study species show regional population declines in only the eastern part of their range. This suggests that phenological mismatches may be resulting in demographic consequences for arctic-nesting birds.

Alaska↗