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Effects of housing density and cage floor space on C57BL/6J mice

The Guide for the Care and Use of Laboratory Animals (the Guide) is widely accepted as the housing standard by most Institutional Animal Care and Use Committees. The recommendations are based on best professional judgment rather than experimental data. Current efforts are directed toward replacing these guidelines with data-driven, species-appropriate standards. Our studies were undertaken to determine the optimum housing density for C57BL/6J mice, the most commonly used inbred mouse strain. Four-week-old mice were housed for 8 weeks at four densities (the recommended ???12 in2 [ca. 77.4 cm 2]/mouse down to 5.6 in2 [ca. 36.1 cm2]/mouse) in three cage types with various amounts of floor space. Housing density did not affect a variety of physiologic parameters but did affect certain micro-environmental parameters, although these remained within accepted ranges. A second study was undertaken housing C57BL/6J mice with as little as 3.2 in2/mouse (ca. 20.6 cm2). The major effect was elevated ammonia concentrations that exceeded limits acceptable in the workplace at increased housing densities; however, the nasal passages and eyeballs of the mice remained microscopically normal. On the basis of these results, we conclude that C57BL/6J mice as large as 29 g may be housed with 5.6 in2 of floor space per mouse. This area is approximately half the floor space recommended in the Guide. The role of the Guide is to ensure that laboratory animals are well treated and housed in a species-appropriate manner. Our data suggest that current policies could be altered in order to provide the optimal habitation conditions matched to this species' social needs. Copyright 2004 by the American Association for Laboratory Animal Science.

Comparative Medicine↗

Building the team for team science

The ability to effectively exchange information and develop trusting, collaborative relationships across disciplinary boundaries is essential for 21st century scientists charged with solving complex and large-scale societal and environmental challenges, yet these communication skills are rarely taught. Here, we describe an adaptable training program designed to increase the capacity of scientists to engage in information exchange and relationship development in team science settings. A pilot of the program, developed by a leader in ecological network science, the Global Lake Ecological Observatory Network (GLEON), indicates that the training program resulted in improvement in early career scientists’ confidence in team-based network science collaborations within and outside of the program. Fellows in the program navigated human-network challenges, expanded communication skills, and improved their ability to build professional relationships, all in the context of producing collaborative scientific outcomes. Here, we describe the rationale for key communication training elements and provide evidence that such training is effective in building essential team science skills.

Ecosphere↗

Ground-water recharge in the arid and semiarid southwestern United States

Ground-water recharge in the arid and semiarid southwestern United States results from the complex interplay of climate, geology, and vegetation across widely ranging spatial and temporal scales. Present-day recharge tends to be narrowly focused in time and space. Widespread water-table declines accompanied agricultural development during the twentieth century, demonstrating that sustainable ground-water supplies are not guaranteed when part of the extracted resource represents paleorecharge. Climatic controls on ground-water recharge range from seasonal cycles of summer monsoonal and winter frontal storms to multimillennial cycles of glacial and interglacial periods. Precipitation patterns reflect global-scale interactions among the oceans, atmosphere, and continents. Large-scale climatic influences associated with El Niño and Pacific Decadal Oscillations strongly, but irregularly, control weather in the study area, so that year-to-year variations in precipitation and ground-water recharge are large and difficult to predict. Proxy data indicate geologically recent periods of naturally occurring multidecadal droughts unlike any in the modern instrumental record. Any anthropogenically induced climate change will likely reduce ground-water recharge through diminished snowpack at higher elevations. Future changes in El Niño and monsoonal patterns, both crucial to precipitation in the study area, are highly uncertain in current models. Current land-use modifications influence ground-water recharge through vegetation, irrigation, and impermeable area. High mountain ranges bounding the study area—the San Bernadino Mountains and Sierra Nevada to the west, and the Wasatch and southern Colorado Rocky Mountains to the east—provide external geologic controls on ground-water recharge. Internal geologic controls stem from tectonic processes that led to numerous, variably connected alluvial-filled basins, exposure of extensive Paleozoic aquifers in mountainous recharge areas, and distinct modes of recharge in the Colorado Plateau and Basin and Range subregions. The chapters in this professional paper present (first) an overview of climatic and hydrogeologic framework (chapter A), followed by a regional analysis of ground-water recharge across the entire study area (chapter B). These are followed by an overview of site-specific case studies representing different subareas of the geographically diverse arid and semiarid southwestern United States (chapter C); the case studies themselves follow in chapters D–K. The regional analysis includes detailed hydrologic modeling within the framework of a high-resolution geographic-information system (GIS). Results from the regional analysis are used to explore both the distribution of ground-water recharge for mean climatic conditions as well as the influence of two climatic patterns—the El Niño-Southern Oscillation and Pacific Decadal Oscillation—that impart a high degree of variability to the hydrologic cycle. Individual case studies employ a variety of geophysical and geochemical techniques to investigate recharge processes and relate the processes to local geologic and climatic conditions. All of the case studies made use of naturally occurring tracers to quantify recharge. Thermal and geophysical techniques that were developed in the course of the studies are presented in appendices. The quantification of ground-water recharge in arid settings is inherently difficult due to the generally low amount of recharge, its spatially and temporally spotty nature, and the absence of techniques for directly measuring fluxes entering the saturated zone from the unsaturated zone. Deep water tables in arid alluvial basins correspond to thick unsaturated zones that produce up to millennial time lags between changes in hydrologic conditions at the land surface and subsequent changes in recharge to underlying ground water. Recent advances in physical, chemical, isotopic, and modeling techniques have fostered new types of recharge assessments. Chemical and isotopic techniques include an increasing variety of environmental tracers that are useful and robust. Physically based techniques include the use of heat as a tracer and computationally intensive geophysical imaging tools for characterizing hydrologic conditions in the unsaturated zone. Modeling-based techniques include spatially distributed water-budget computations using high-resolution remotely sensed and ground-based geographic data. Application of these techniques to arid and semiarid settings in the southwestern United States reveals distinct patterns of recharge corresponding to geologic setting, climatic and vegetative history, and land use. Analysis of recharge patterns shows that large expanses of alluvial basin floors are drying out under current climatic conditions, with little to no recharge to underlying ground water. Ground-water recharge occurs mainly beneath upland catchments in which thin soils overlie permeable bedrock, ephemeral channels in which flow may average only several hours per year, and active agricultural areas. The chapters in this professional paper represent a coordinated attempt to develop a better understanding of one of the Nation's most critical yet difficult-to-quantify renewable resources.

Professional Paper↗

U.S. Geological Survey Activities Related to American Indians and Alaska Natives Fiscal Year 2003

Introduction Information is a resource for Native American governments, communities, organizations, and people. The U.S. Geological Survey (USGS) provides technical expertise, reports, and other impartial information sources that benefit Native Americans interested in subsistence issues, water, resources and land use, and the health of many parts of the environment. Native decisions based in USGS data and analyses support the goals of Native self-sufficiency, economic development, and conservation. The USGS works in cooperation with American Indian and Alaska Native governments, conducting research on: water energy and mineral resources; animals and plants of environmental, economic, or subsistence importance; and natural hazards. Digital data on cartography, energy and mineral resources, streamflow, biota, and other topics are available to American Indian and Alaska Native individuals and institutions. The USGS also recognizes the need to learn from and share knowledge with Native peoples. This report describes most of the activities that the USGS conducted with American Indian and Alaska Native governments, educational institutions, and individuals during Federal fiscal year (FY) 2003. Some of these USGS activities were conducted cooperatively with the Bureau of Indian Affairs (BIA) or other Federal entities. Others were collaborations with Tribes, Tribal organizations, and professional societies.

Report↗

Geologic mapping of Kentucky: A history and evaluation of the Kentucky Geological Survey--U.S. Geological Survey Mapping Program, 1960-1978

In 1960, the U.S. Geological Survey and the Kentucky Geological Survey began a program to map the State geologically at a scale of 1:24,000 and to publish the maps as 707 U.S. Geological Survey Geologic Quadrangle Maps. Fieldwork was completed by the spring of 1977, and all maps were published by December 1978. Geologic mapping of the State was proposed by the Kentucky Society of Professional Engineers in 1959. Wallace W. Hagan, Director and State Geologist of the Kentucky Geological Survey, and Preston McGrain, Assistant State Geologist, promoted support for the proposal among organizations such as Chambers of Commerce, industrial associations, professional societies, and among members of the State government. It was also arranged for the U.S. Geological Survey to supply mapping personnel and to publish the maps; the cost would be shared equally by the two organizations. Members of the U.S. Geological Survey assigned to the program were organized as the Branch of Kentucky Geology. Branch headquarters, including an editorial staff, was at Lexington, Ky., but actual mapping was conducted from 18 field offices distributed throughout the State. The Publications Division of the U.S. Geological Survey established a cartographic office at Lexington to prepare the maps for publication. About 260 people, including more than 200 professionals, were assigned to the Branch of Kentucky Geology by the U.S. Geological Survey at one time or another. The most geologists assigned any one year was 61. To complete the mapping and ancillary studies, 661 professional man-years were required, compared with an original estimate of 600 man-years. A wide variety of field methods were used, but most geologists relied on the surveying altimeter to obtain elevations. Surface data were supplemented by drill-hole records, and several dozen shallow diamond-drill holes were drilled to aid the mapping. Geologists generally scribed their own maps, with a consequent saving of publication costs. Paleontologists and stratigraphers of the U.S. Geological Survey cooperated closely with the program. Paleontologic studies were concentrated in the Ordovician of central Kentucky, the Pennsylvanian of eastern and western Kentucky, and the Mesozoic and Cenozoic of westernmost Kentucky. In addition to financial support, the Kentucky Geological Survey provided economic data, stratigraphic support, and drillhole records to the field offices. Geologists of the State Survey made subsurface structural interpretations, constructed bedrock topography maps, and mapped several quadrangles. Some of the problems encountered were the inadequacy of much of the existing stratigraphic nomenclature, the uneven quality of some of the mapping, and the effects of relative isolation on the professional development of some of the geologists. The program cost a total of $20,927,500. In terms of 1960 dollars, it cost $16,035,000; this compares with an original estimate of $12,000,000. Although it is difficult to place a monetary value on the geologic mapping, the program has contributed to newly discovered mineral wealth, jobs, and money saved by government and industry. The maps are used widely in the exploration for coal, oil and gas, fluorspar, limestone, and clay. The maps are also used in planning highways and locations of dams, in evaluating foundation and excavation conditions, in preparing environmental impact statements, and in land-use planning.

Kentucky↗

Dynamics of land-use change and conservation in the Prairie Pothole Region of the United States: environmental and economic implications with linkages to rural community well-being

Rural America has changed dramatically over the last century, from having over half the population living in rural settings to only 20 percent residing in a rural area today, and outmigration of younger populations from rural communities remains a constant issue for local governing officials. A declining tax base and concurrent rising costs for maintenance and repair of aging infrastructure add further challenges to policy decisions. Reduced enrollment has caused school closures or mergers. Farm consolidation and technical advances reduced the demand for local labor. On the positive side, however, record-high commodity prices have amplified farm income to new heights. The increased revenues can lead to farmers spending additional money within the local region, while at the same time increased transportation of products has impacted local infrastructure such as roads and bridges. Such dynamics present challenges for municipal leaders charged with promoting economic development and balanced spending, while at the same time maintaining the way of life and rural character that are so important to area residents. The Prairie Pothole Region (PPR) of the United States covers much of the Northern Great Plains, including parts of North and South Dakota, Minnesota, Iowa, and a small part of Montana, and extends across a broad swath of Alberta and Saskatchewan. The region is defined largely by its rural character but has experienced extensive land conversion over the last century, with agricultural areas replacing native prairie habitat. Additional pressures arise from oil and gas development, global markets for agricultural production, and increased demands for biofuel feedstocks. Record-high commodity prices increase pressure on the native prairie as farmers look for new cropland acres. The volatility of commodity prices has raised fears over the intensity of land conversion to row-crop agriculture, the economic health and resiliency of rural communities, and ultimately, population dynamics and outmigration of younger generations. Land-use pressures are increased by the exponential growth of oil and gas production in the region, where some 8,200 wells are now in production within the Williston Basin of North Dakota, accompanied by increased population pressures on housing and municipal services. The U.S. Department of Agriculture’s Conservation Reserve Program (CRP)--a cropland retirement program with close to 4.8 million acres enrolled in the PPR--faces uncertainty in upcoming legislative actions, with a large majority of property enrollments scheduled to expire by 2017. The CRP historically has provided improved habitat conditions, reductions of soil damage through erosion and loss of nutrients, and sequestration of millions of tons of atmospheric carbon. In turn, wildlife-related recreation levels have increased in many parts of the PPR, with money spent in local communities. Contemporary resource-management and rural-development planning increasingly emphasize the need for diversification and integration of resource-extractive industries with nonmarket-based recreational and amenity values that tie into quality of life. Ultimately, each community is unique in its environmental, social, economic, and fiscal endowments. One rural-development policy may work better in one community than another. In addition, rural-development issues such as migration, job growth, and taxes are diverse in themselves. The goal of this report is to qualitatively and quantitatively discuss the economic impacts of land-use decisions in rural areas, particularly in the PPR.

Professional Paper↗

Climate change effects on North American fish and fisheries to inform adaptation strategies

Climate change is a global persistent threat to fish and fish habitats throughout North America. Climate-induced modification of environmental regimes, including changes in streamflow, water temperature, salinity, storm surges, and habitat connectivity can change fish physiology, disrupt spawning cues, cause fish extinctions and invasions, and alter fish community structure. Reducing greenhouse emissions remains the primary mechanism to slow the pace of climate change, but local and regional management agencies and stakeholders have developed an arsenal of adaptation strategies to help partially mitigate the effects of climate change on fish. We summarize common stressors posed by climate change in North America, including (1) increased water temperature, (2) changes in precipitation, (3) sea level rise, and (4) ocean acidification, and present potential adaptation strategies that fishery professionals may apply to help vulnerable fish and fisheries cope with a changing climate. Although our adaptation strategies are primarily from North America, they have broader geographic applicability to fish and aquatic biota in other jurisdictions. These strategies provide opportunities for managers to mitigate the effects of climate change on fish and fish habitat while needed global policies to reduce greenhouse gas emissions emerge, which may offer more lasting solutions.

Fisheries Magazine↗

National Park Service Vegetation Mapping Inventory Program: Jean Lafitte National Historical Park and Preserve Vegetation Mapping Project

The National Park Service, Natural Resource Stewardship and Science office in Fort Collins, Colorado, publishes a range of reports that address natural resource topics. These reports are of interest and applicability to a broad audience in the National Park Service and others in natural resource management, including scientists, conservation and environmental constituencies, and the public. The Natural Resource Report Series is used to disseminate comprehensive information and analysis about natural resources and related topics concerning lands managed by the National Park Service. The series supports the advancement of science, informed decision-making, and the achievement of the National Park Service mission. The series also provides a forum for presenting lengthier results that may not be accepted by publications with page limitations. All manuscripts in the series receive the appropriate level of peer review to ensure that the information is scientifically credible, technically accurate, appropriately written for the intended audience, and designed and published in a professional manner. This report received formal peer review by subject-matter experts whose background and expertise put them on par technically and scientifically with the authors of the information. The peer review was led according to the Fundamental Science Practices of the U.S. Geological Survey. Views, statements, findings, conclusions, recommendations, and data in this report do not necessarily reflect views and policies of the National Park Service, U.S. Department of the Interior. Mention of trade names or commercial products does not constitute endorsement or recommendation for use by the U.S. Government. This report is available in digital format from the Gulf Coast Network website and the Natural Resource Publications Management website.

Louisiana↗

Tracking fisheries through time: The American Fisheries Society as an historical lens

The historical context of current environment conditions offers vital guidance to North American fisheries professionals as they strive to develop effective management plans and policies. This retrospective includes understanding the remarkable historic fisheries resource and causative reasons for establishing their public fisheries agencies and the American Fisheries Society (Society). Through a review of primary historical documents and literature for North America, this paper identifies: factors contributing to the of founding of the Society and public fish commissions; shows how selected resource issues evolved; and documents how and why selected fisheries and aquatic habitat policies changed in response to those issues. Overexploitation, landscape-scale habitat alterations, mining, and dams were the causative agents for emergence of fisheries agencies and the Society, and these environmental factors remain relevant today. Beginning in the 1960s, after 100 years of inaction, North Americans grew tired of degraded waters and fisheries and forced policy changes that have directly and indirectly affected the fisheries we manage today. The historical events and resulting corrective legislation are taken for granted by those unaware that our Society has actively participated in developing policies to address these environmental harms. Further, the Society continues to play a vital role in identifying key issues, providing conduits for information to cope with impairments, and advocating for policies to conserve intact habitats and improve degraded systems. The struggles documented in this paper offer crucial lessons as we continue to be challenged by legacy resource issues and face emerging environmental stressors such as climate change as well as regression in long-standing environmental protection policies.

Fisheries Magazine↗

U.S. Geological Survey Activities Related to American Indians and Alaska Natives: Fiscal Year 2005

Introduction This report describes the activities that the U.S. Geological Survey (USGS) conducted with American Indian and Alaska Native governments, educational institutions, and individuals during Federal fiscal year (FY) 2005. Most of these USGS activities were collaborations with Tribes, Tribal organizations, or professional societies. Others were conducted cooperatively with the Bureau of Indian Affairs (BIA) or other Federal entities. The USGS is the earth and natural science bureau within the U.S. Department of the Interior (DOI). The USGS does not have regulatory or land management responsibilities. As described in this report, there are many USGS activities that are directly relevant to American Indians, Alaska Natives, and to Native lands. A USGS website, dedicated to making USGS more accessible to American Indians, Alaska Natives, their governments, and institutions, is available at www.usgs.gov/indian. This website includes information on how to contact USGS American Indian/Alaska Native Liaisons, training opportunities, and links to other information resources. This report and previous editions are also available through the website. The USGS realizes that Native knowledge and cultural traditions of living in harmony with nature result in unique Native perspectives that enrich USGS studies. USGS seeks to increase the sensitivity and openness of its scientists to the breadth of Native knowledge, expanding the information on which their research is based. USGS scientific studies include data collection, mapping, natural resource modeling, and research projects. These projects typically last 2 or 3 years, although some are parts of longer-term activities. Some projects are funded cooperatively, with USGS funds matched or supplemented by individual Tribal governments, or by the BIA. These projects may also receive funding from the U.S. Environmental Protection Agency (USEPA), the Indian Health Service (part of the Department of Health and Human Services), or other Federal agencies. The USGS routinely works with its sister bureaus in the Department of the Interior to provide the scientific information and expertise needed to meet the Department's science priorities. Some USGS activities described in this report are conducted as collateral tasks that result from USGS employees identifying and responding to perceived needs. These endeavors are usually prompted by employee interests and frequently involve educational activities. The education is often a reciprocal learning and teaching experience for USGS employees and for Native participants. Through these activities, USGS employees help to fulfill a mission of the USGS - to demonstrate scientific relevance - while helping their fellow citizens. Increasingly, some of the educational activities are becoming parts of formal USGS projects. USGS employees also take initiative in assisting American Indians and Alaska Natives by participating in several organizations that promote awareness of science career opportunities among Native peoples and help build support and communication networks. One such group is the American Indian Science and Engineering Society (AISES). USGS employees join this organization on a voluntary basis, bringing the benefits of this expanded network to the USGS, as many employees do with other professional organizations. The studies briefly described in this report span subsistence issues, wildlife health, water quality, mineral resources, monitoring and modeling to gather information and predict what may happen in the future. Although each project description relates to Native Americans in some way, the projects vary widely, including who conducted the work, the goals and products, the duration of the study, and whether it was local or covered a broad area. Each major organizational unit of the USGS has identified an American Indian/Alaska Native liaison. The USGS has a regional organizational structure, with Western, Central,

Circular↗

Environmental considerations related to mining of nonfuel minerals

Throughout most of human history, environmental stewardship during mining has not been a priority partly because of the lack of applicable laws and regulations and partly because of ignorance about the effects that mining can have on the environment. In the United States, the National Environmental Policy Act of 1969, in conjunction with related laws, codified a more modern approach to mining, including the responsibility for environmental stewardship, and provided a framework for incorporating environmental protection into mine planning. Today, similar frameworks are in place in the other developed countries of the world, and international mining companies generally follow similar procedures wherever they work in the world. The regulatory guidance has fostered an international effort among all stakeholders to identify best practices for environmental stewardship. The modern approach to mining using best practices involves the following: (a) establishment of a pre-mining baseline from which to monitor environmental effects during mining and help establish geologically reasonable closure goals; (b) identification of environmental risks related to mining through standardized approaches; and (c) formulation of an environmental closure plan before the start of mining. A key aspect of identifying the environmental risks and mitigating those risks is understanding how the risks vary from one deposit type to another—a concept that forms the basis for geoenvironmental mineral-deposit models. Accompanying the quest for best practices is the goal of making mining sustainable into the future. Sustainable mine development is generally considered to be development that meets the needs of the present generation without compromising the ability of future generations to meet their own needs. The concept extends beyond the availability of nonrenewable mineral commodities and includes the environmental and social effects of mine development. Global population growth, meanwhile, has decreased the percentage of inhabitable land available to support society’s material needs. Presently, the land area available to supply the mineral resources, energy resources, water, food, shelter, and waste disposal needs of all Earth’s inhabitants is estimated to be 135 square meters per person. Continued global population growth will only increase the challenges of sustainable mining. Current trends in mining are also expected to lead to new environmental challenges in the future, among which are mine-waste management issues related to mining larger deposits for lower ore grade; water-management issues related to both the mining of larger deposits and the changes in precipitation brought about by climate change; and greenhouse gas issues related to reducing the carbon footprint of larger, more energy-intensive mining operations.

Professional Paper↗

State of the Earth’s cryosphere at the beginning of the 21st century: Glaciers, global snow cover, floating ice, and permafrost and periglacial environments

This chapter is the tenth in a series of 11 book-length chapters, collectively referred to as “this volume,” in the series U.S. Geological Survey Professional Paper 1386, Satellite Image Atlas of Glaciers of the World. In the other 10 chapters, each of which concerns a specific glacierized region of Earth, the authors used remotely sensed images, primarily from the Landsat 1, 2, and 3 series of spacecraft, in order to analyze that glacierized region and to monitor changes in its glaciers. Landsat images, acquired primarily during the period 1972 through 1981, were used by an international team of glaciologists and other scientists to study the various glacierized regions and (or) to discuss related glaciological topics. In each glacierized region, the present distribution of glaciers within its geographic area is compared, wherever possible, with historical information about their past areal extent. The atlas provides an accurate regional inventory of the areal extent of glacier ice on our planet during the 1970s as part of an expanding international scientific effort to measure global environmental change on the Earth’s surface. However, this chapter differs from the other 10 in its discussion of observed changes in all four elements of the Earth’s cryosphere (glaciers, snow cover, floating ice, and permafrost) in the context of documented changes in all components of the Earth System. Human impact on the planet at the beginning of the 21st century is pervasive. The focus of Chapter A is on changes in the cryosphere and the importance of long-term monitoring by a variety of sensors carried on Earth-orbiting satellites or by a ground-based network of observatories in the case of permafrost. The chapter consists of five parts. The first part provides an introduction to the Earth System, including the interrelationships of the geosphere (cryosphere, hydrosphere, lithosphere, and atmosphere), the biosphere, climate processes, biogeochemical cycles, and the critically important hydrologic cycle, in which glacier ice is the second largest reservoir of water after the oceans. The second part assesses the state of glaciers in all of the glacierized regions of the planet, primarily as drawn in the other 10 chapters. It includes sections on ice cores and the climate record they contain, volumetric changes in glaciers, harnessing spaceborne sensors to measure changes in glaciers, and related topics. The third part summarizes trends in global snow cover. The fourth part summarizes long-term changes in area and thickness of floating ice, including polar sea ice and freshwater (lake and river) ice. The fifth part assesses the loss of permafrost and changes in periglacial environments at high latitudes and high altitudes.

Professional Paper↗

Establishing aquatic restoration priorities using a watershed approach

Since the passage of the Clean Water Act in 1972, the United States has made great strides to reduce the threats to its rivers, lakes, and wetlands from pollution. However, despite our obvious successes, nearly half of the nation’s surface water resources remain incapable of supporting basic aquatic values or maintaining water quality adequate for recreational swimming. The Clean Water Act established a significant federal presence in water quality regulation by controlling point and non-point sources of pollution. Point-sources of pollution were the major emphasis of the Act, but Section 208 specifically addressed non-point sources of pollution and designated silviculture and livestock grazing as sources of non-point pollution. Non-point source pollutants include runoff from agriculture, municipalities, timber harvesting, mining, and livestock grazing. Non-point source pollution now accounts for more than half of the United States water quality impairments. To successfully improve water quality, restoration practitioners must start with an understanding of what ecosystem processes are operating in the watershed and how they have been affected by outside variables. A watershed-based analysis template developed in the Pacific Northwest can be a valuable aid in developing that level of understanding. The watershed analysis technique identifies four ecosystem scales useful to identify stream restoration priorities: region, basin, watershed, and site. The watershed analysis technique is based on a set of technically rigorous and defensible procedures designed to provide information on what processes are active at the watershed scale, how those processes are distributed in time and space. They help describe what the current upland and riparian conditions of the watershed are and how these conditions in turn influence aquatic habitat and other beneficial uses. The analysis is organized as a set of six steps that direct an interdisciplinary team of specialists to examine the biotic and abiotic processes influencing aquatic habitat and species abundance. This process helps develop an understanding of the watershed within the context of the larger ecosystem. The understanding gained can then be used to identify and prioritize aquatic restoration activities at the appropriate temporal and spatial scale. The watershed approach prevents relying solely on site-level information, a common problem with historic restoration efforts. When the watershed analysis process was used in the Whitefish Mountains of northwest Montana, natural resource professionals were able to determine the dominant habitat forming processes important for native fishes and use that information to prioritize, plan, and implement the appropriate restoration activities at the watershed scale. Despite considerable investments of time and resources needed to complete an analysis at the watershed scale, the results can prevent the misdiagnosis of aquatic problems and help ensure that the objectives of aquatic restoration will be met.

Journal of Environmental Management↗

Guidance documents: Continued support to improve operations of fish hatcheries and field sites to reduce the impact or prevent establishment of New Zealand Mudsnails and other invasive mollusks

This project tested and revised a risk assessment/management tool authored by Moffitt and Stockton designed to provide hatchery biologists and others a structure to measure risk and provide tools to control, prevent or eliminate invasive New Zealand mudsnails (NZMS) and other invasive mollusks in fish hatcheries and hatchery operations. The document has two parts: the risk assessment tool, and an appendix that summarizes options for control or management. The framework of the guidance document for risk assessment/hatchery tool combines approaches used by the Hazard Analysis and Critical Control Points (HACCP) process with those developed by the Commission for Environmental Cooperation (CEC), of Canada, Mexico, and the United States, in the Tri-National Risk Assessment Guidelines for Aquatic Alien Invasive Species. The framework approach for this attached first document assesses risk potential with two activities: probability of infestation and consequences of infestation. Each activity is treated equally to determine the risk potential. These two activities are divided into seven basic elements that utilize scientific, technical, and other relevant information in the process of the risk assessment. To determine the probability of infestation four steps are used that have scores reported or determined and averaged. This assessment follows a familiar HACCP process to assess pathways of entry, entry potential, colonization potential, spread potential. The economic, environmental and social consequences are considered as economic impact, environmental impact, and social and cultural influences. To test this document, the Principal Investigator worked to identify interested hatchery managers through contacts at regional aquaculture meetings, fish health meetings, and through the network of invasive species managers and scientists participating in the Western Regional Panel on Aquatic Nuisance Species and the 100th Meridian Initiative's Columbia River Basin Team, and the Western New Zealand Mudsnail Conference in Seattle. Targeted hatchery workshops were conducted with staff at Dworshak National Fish Hatchery Complex (ID), Similkameen Pond, Oroville WA, and Ringold Springs State Hatchery (WA). As a result of communications with hatchery staff, invasive species managers, and on site assessments of hatchery facilities, the document was modified and enhanced. Additional resources were added to keep it up to date. The result is a more simplified tool that can lead hatchery or management personnel through the process of risk assessment and provide an introduction to the risk management and communication process. In addition to the typical HACCP processes, this tool adds steps to rate and consider uncertainty and the weight of evidence regarding options and monitoring results . Uncertainty of outcome exists in most tools that can be used to control or prevent NZMS or other invasive mollusks from infesting an area. In additional this document emphasizes that specific control tools and plans must be tailored to each specific setting to consider the economic, environmental and social influences. From the testing and evaluation process, there was a strong recognition that a number of control and prevention tools previously suggested and reported in the literature from laboratory and small scale trials may not be compatible with regional and national regulations, economic constraints, social or cultural constraints, engineering or water chemistry characteristics of each facility. The options for control are summarized in the second document, Review of Control Measures for Hatcheries Infested with NZMS (Appendix A) that provides sources for additional resources and specific tools, and guidance regarding the feasibility and success of each approach. This tool also emphasizes that management plans need to be adaptive and incorporate oversight from professionals familiar with measuring risks of fish diseases, and treatments (e.g. the fish health practitioners and water quality and effluent management teams). Finally, with such a team, the adaptive management approach must be ongoing, and become a regular component of hatchery operations. Although it was the intent that this two part document would be included as part of the revised National Management and Control Plan for the NZMS proposed by the U.S. Fish and Wildlife Service (USFWS) and others, it is provided as a stand-alone document.

Cooperator Science Series↗

Perceptions of strengths and deficiencies: Disconnects between graduate students and prospective employers

The US Botanical Capacity Assessment Project (BCAP) was initiated as a first step to gauge the nation's collective ability to meet the environmental challenges of the 21st century. The project, in which the authors of this article are involved, specifically aimed to identify multisector contributions to and gaps in botanical capacity in order to develop growth opportunities to address research and management problems. One of the primary gaps revealed by the BCAP surveys was that the skills graduate students identified as their greatest strengths closely matched the areas future employers (government and private sectors) identified as needing greatest improvement. Although our survey focused on only one discipline (botany), we suspect that the results are applicable throughout the science, technology, engineering, and mathematics disciplines. We suggest that it is critical for university faculty and administrators to team with professionals from government, nonprofit, and for-profit organizations to identify critical and desired knowledge and skill sets and implement the necessary curriculum changes to provide graduates with the tools they need.

BioScience↗

Mussel community assessment tool for the Upper Mississippi River system

Upper Mississippi River (UMR) resource managers need a quantitative means of evaluating the health of mussel assemblages to measure effects of management and regulatory actions, assess restoration techniques, and inform regulatory tasks. Our objective was to create a mussel community assessment tool (MCAT), consisting of a suite of metrics and scoring criteria, to consistently compare the relative health of UMR mussel assemblages. We developed an initial MCAT using quantitative data from 25 sites and 10 metrics. Metrics fell in five broad groups: conservation status and environmental sensitivity, taxonomic composition, population processes, abundance, and diversity. Metric scoring categories were based on quartile analysis: 25% scoring as good, 50% scoring as fair, and 25% scoring as poor. Scores were meant to facilitate establishing management priorities and mitigation options for the conservation of mussels. Scoring categories assumed that a healthy mussel assemblage consists of species with a variety of reproductive and life-history strategies, a low percentage of tolerant species, and a high percentage of sensitive species; shows evidence of adequate recruitment, a variety of age classes, and low mortality; and has high abundance, species richness, and species and tribe evenness. Metrics were validated using a modified Delphi technique. MCAT metrics generally reflected the professional opinions of UMR resource managers and provided a consistent evaluation technique with uniform definitions that managers could use to evaluate mussel assemblages. Additional data sets scored a priori by UMR resource managers were used to further validate metrics, resulting in data from 33 sites spanning over 980 km of the UMR. Initial and revised MCAT scores were similar, indicating that data represent the range of mussel assemblages in the UMR. Mussel assemblages could be evaluated using individual metrics or a composite score to suit management purposes. With additional data, metrics could be calibrated on a local scale or applied to other river systems.

Illinois, Iowa, Minnesota, Missouri, Wisconsin↗

Blue Mountains Ecoregion: Chapter 16 in Status and trends of land change in the Western United States--1973 to 2000

The Blue Mountains Ecoregion encompasses approximately 65,461 km² (25,275 mi²) of land bordered on the north by the Columbia Plateau Ecoregion, on the east by the Northern Rockies Ecoregion, on the south by the Snake River Basin and the Northern Basin and Range Ecoregions, and on the west by the Cascades and the Eastern Cascades Slopes and Foothills Ecoregions (fig. 1) (Omernik, 1987; U.S. Environmental Protection Agency, 1997). Most of the Blue Mountains Ecoregion is located within Oregon (83.5 percent); 13.8 percent is in Idaho, and 2.7 percent is in Washington. The Blue Mountains are composed of primarily Paleozoic volcanic rocks, with minor sedimentary, metamorphic, and granitic rocks. Lower mountains and numerous basin-and-range areas, as well as the lack of Quaternary-age volcanoes, distinguish the Blue Mountains from the adjacent Cascade Range (Thorson and others, 2003).

Idaho;Oregon;Washington↗

The effects of management practices on grassland birds—Greater Sage-Grouse ( Centrocercus urophasianus )

Keys to Greater Sage-Grouse ( Centrocercus urophasianus ) management are maintenance of expansive stands of sagebrush ( Artemisia species [spp.]), especially varieties of big sagebrush ( Artemisia tridentata ) with abundant forbs in the understory, particularly during spring; undisturbed and somewhat open sites for leks; and healthy perennial grass and forb stands intermixed with sagebrush for brood rearing. Within suitable habitats, areas should have 15–25 percent canopy cover of sagebrush 30–80 centimeters (cm) tall for nesting and 10–25 percent canopy cover 40–80 cm tall for brood rearing. In winter habitats, shrubs should be exposed 25–35 cm above snow and have 10–30 percent canopy cover exposed above snow. In nesting and brood-rearing habitats, the understory should have at least 15 percent cover of grasses and at least 10 percent cover of forbs greater than or equal to 18 cm tall. Greater Sage-Grouse have been reported to use habitats with 5–110 cm average vegetation height, 5–160 cm visual obstruction reading, 3–51 percent grass cover, 3–20 percent forb cover, 3–69 percent shrub cover, 7–63 percent sagebrush cover, 14–51 percent bare ground, and 0–18 percent litter cover. Unless otherwise noted, this account refers to habitat requirements and environmental factors affecting Greater Sage-Grouse but not Gunnison Sage-Grouse ( Centrocercus minimus ). Habitats used by Gunnison Sage-Grouse are generally similar to habitats used by Greater Sage-Grouse, but some differences have been reported. The Greater Sage-Grouse is a game bird and is hunted throughout most of its current range. This account does not address harvest or its effects on populations; rather, this account focuses on the effects of habitat management.

Professional Paper↗