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USGS2017

Identifying ecologically relevant scales of habitat selection: diel habitat selection in elk

Although organisms make resource selection decisions at multiple spatiotemporal scales, not all scales are ecologically relevant to any given organism. Ecological patterns and rhythms such as behavioral and climatic patterns may provide a consistent method for identifying ecologically relevant scales of habitat selection. Using elk ( Cervus canadensis ) as an example species, we sought to test the ability of behavioral patterns to empirically partition diel scales for modeling habitat selection. We used model selection to partition diel scales by shifts in dominant behavior and then used resource selection probability functions to model elk habitat selection hierarchically at diel scales within seasons. Model selection distinguished four diel temporal partitions following elk crepuscular behavioral patterns: dawn, midday, dusk, and night. Across seasons, model‐averaged coefficients indicated that elk shifted from selecting grassland cover at dawn/dusk, to selecting for greater canopy and forest cover at midday, and then to areas with greater herbaceous biomass at night. Top models changed between diel intervals in spring and fall but stayed the same across diel intervals in winter and summer. In winter, elk selected for southern aspects during midday, for unburned areas at dawn/dusk, and for areas burned within 1–3 yr at dawn/dusk and night. In spring, elk selected for northern aspects and for areas burned within 1–3 yr at midday, for areas farther from roads at dawn/dusk and midday, and for areas farther from water at midday. In summer, elk changed diel preferences for fewer covariates: At dawn/dusk and midday, elk selected for areas farther from water and avoided forest cover, and at night, elk selected for areas burned within 1–3 yr. In fall, elk selected for areas burned the previous year at dawn/dusk and night, for higher elevations at midday, and for areas closer water at night. Using behavioral patterns to identify ecologically relevant scales can help identify overlooked habitat requirements such as diel changes in preference for fire history, forage availability, and cover. We show that the ecological relevancy of a given scale (e.g., a diel temporal scale) can change throughout a given extent (e.g., across seasons).

New Mexico↗

USGS1999

Relation of pesticide concentrations to season, streamflow, and land use in seven New Jersey streams

The presence and variability of pesticides in seven New Jersey streams was documented by analyzing 146 samples collected from the streams from April 1996 through June 1998. The samples were analyzed for 85 pesticides, including 50 herbicides, 28 insecticides, and 7 degradation products, at method detection limits that ranged from 0.001 to 0.018 μg/L (micrograms per liter). Pesticides were frequently detected; however, concentrations were generally low. The pesticides most frequently detected were atrazine, in 97 percent of the samples; prometon, 96 percent; metolachlor, 95 percent; desethyl-atrazine, 91 percent; simazine, 88 percent; diazinon, 58 percent; alachlor, 56 percent; and carbaryl, 54 percent. Detection frequencies were highest during the growing season (April-September). At least one pesticide was detected in all but one of these samples, and 49 percent of the samples contained 9 or more pesticides. The numbers of pesticides detected at a given site ranged from 13 to 29. Ten pesticides were detected at concentrations that exceeded established water-quality criteria. Thirty-one of these detections were in samples collected during the growing season and one during the nongrowing season. The pesticides that exceeded the U.S. Environmental Protection Agency (USEPA) maximum contaminant level for drinking water were atrazine, which exceeded 3 μg/L in four samples, and alachlor, 2 μg/L in two samples. Cyanazine exceeded the USEPA liftime health advisory level (HAL) of 1 μg/L in two samples. These eight detections occurred during runoff shortly after spring pesticide applications and represent a potential threat to municipal water supplies in the Raritan River basin. Concentrations of chlorpyrifos, chlorthalonil, diazinon, ethyl-parathion, and methyl-azinphos exceeded the chronic life criteria for the protection of aquatic life (ACQR) in 20 samples at four sites during the growing season. Dieldrin was detected in four samples and DDE in two samples at concentrations that exceeded New Jersey Department of Environmental Protection (NJDEP) human health criteria. Individual and total-pesticide concentrations and total numbers of pesticides detected in the samples varied with season and flow conditions. Median and maximum concentrations of most of the pesticides were highest during runoff in the growing season. Pesticide concentrations were typically lower and less variable in the nongrowing season than in the growing season, regardless of changes in hydrologic conditions; however, median concentrations of most pesticides were slightly lower during runoff than during base flow. The median total-pesticide concentration and median total number of pesticides detected were highest and most variable in runoff samples in the growing season. In the nongrowing season, the median total-pesticide concentration was lowest in runoff samples and least variable during base-flow conditions. Median total numbers of pesticides were lowest and least varibale in the nongrowing season during base-flow conditions at most sites. The highest total-pesticide concentrations were detected in samples from the two small agricultural basins (greater than 25 percent of land use is agricultural) during runoff in late spring and early summer. In general, insecticides were detected more frequently and in greater concentrations at urban sites. Concentrations of agricultural herbicides generally decreased with increasing flow at the four sites with less than 10 percent agriculture land use and increased with increasing flow at the three sites with more than 25 percent agricultural land use. Most of the pesticides that correlated positively with streamflow were detected at sites where land use in the basin would indicate the use of those particular pesticides. Most of the pesticides that correlated negatively with streamflow were present at the site in the Coastal Plain or at sites in which the land use in the basin would not indicate heavy u

New Jersey;New York↗

USGS2003

Orbital control of low-latitude seasonality during the Eemian

We used Sr/Ca and stable isotope data from well dated and preserved corals from the northeastern Caribbean to determine the seasonal environmental conditions for four continuous years during the Eemian, the last time the Earth was in a prolonged warm phase. We determined that the seasonal range in SST during the Eemian was 25°–30° C. This is ∼1–2° larger than at present and caused primarily by winter cooling and, only to a small degree, by summer warming. As climate modeling studies indicate, the bias towards colder winters can be explained by changes in low latitude insolation induced by altered orbital parameters, modulated by atmospheric CO 2 levels that were lower than today. Milankovitch forcing at higher latitudes was probably less important.

Geophysical Research Letters↗

USGS2007

Concentrations of selected pharmaceuticals and antibiotics in south-central Pennsylvania waters, March through September 2006

This report presents environmental and quality-control data from analyses of 15 pharmaceutical and 31 antibiotic compounds in water samples from streams and wells in south-central Pennsylvania. The analyses are part of a study by the U.S. Geological Survey (USGS) in cooperation with the Pennsylvania Department of Environmental Protection (PADEP) to define concentrations of selected emerging contaminants in streams and well water in Pennsylvania. Sampling was conducted at 11 stream sites and at 6 wells in 9 counties of south-central Pennsylvania. Five of the streams received municipal wastewater and 6 of the streams received runoff from agricultural areas dominated by animal-feeding operations. For all 11 streams, samples were collected at locations upstream and downstream of the municipal effluents or animal-feeding operations. All six wells were in agricultural settings. A total of 120 environmental samples and 21 quality-control samples were analyzed for the study. Samples were collected at each site in March/April, May, July, and September 2006 to obtain information on changes in concentration that could be related to seasonal use of compounds. For streams, 13 pharmaceuticals and 11 antibiotics were detected at least 1 time. Detections included analytical results that were estimated or above the minimum reporting limits. Seventy-eight percent of all detections were analyzed in samples collected downstream from municipal-wastewater effluents. For streams receiving wastewater effluents, the pharmaceuticals caffeine and para-xanthine (a degradation product of caffeine) had the greatest concentrations, 4.75 μg/L (micrograms per liter) and 0.853 μg/L, respectively. Other pharmaceuticals and their respective maximum concentrations were carbamazepine (0.516 μg/L) and ibuprofen (0.277 μg/L). For streams receiving wastewater effluents, the antibiotic azithromycin had the greatest concentration (1.65 μg/L), followed by sulfamethoxazole (1.34 μg/L), ofloxacin (0.329 μg/L), and trimethoprim (0.256 μg/L). For streams receiving runoff from animal-feeding operations, the only pharmaceuticals detected were acetaminophen, caffeine, cotinine, diphenhydramine, and carbamazepine. The maximum concentration for pharmaceuticals was 0.053 μg/L. Three streams receiving runoff from animal-feeding operations had detections of one or more antibiotic compound--oxytetracycline, sulfadimethoxine, sulfamethoxazole, and tylosin. The maximum concentration for antibiotics was 0.157 μg/L. The average number of compounds (pharmaceuticals and antibiotics) detected in sites downstream from animal-feeding operations was three. The average number of compounds detected downstream from municipal-wastewater effluents was 13. For wells used to supply livestock, four compounds were detected--two pharmaceuticals (cotinine and diphenhydramine) and two antibiotics (tylosin and sulfamethoxazole). There were five detections in all the well samples. The maximum concentration detected in well water was for cotinine, estimated to be 0.024 μg/L. Seasonal occurrence of pharmaceutical and antibiotic compounds in stream water varied by compound and site type. At four stream sites, the same compounds were detected in all four seasonal samples. At other sites, pharmaceutical or antibiotic compounds were detected only one time in seasonal samples. Winter samples collected in streams receiving municipalwastewater effluent had the greatest number of compounds detected (21). Research analytical methods were used to determine concentrations for pharmaceuticals and antibiotics. To assist in evaluating the quality of the analyses, detailed information is presented on laboratory methodology and results from qualitycontrol samples. Quality-control data include results for nine blanks, nine duplicate environmental sample pairs, and three laboratory-spiked environmental samples as well as the recoveries of compounds in laboratory surrogates and laboratory reagent spikes.

Pennsylvania↗

USGS2026

Seasonal and decadal changes in winter body condition of four sympatric diving ducks

Winter body condition is an important driver of survival, reproductive output, and overall population health in waterfowl. Diving duck species use distinct habitats, exploit unique resources, and can collectively provide an integrated index of winter habitat quality. The San Francisco Bay (SFB) is the largest estuary on the west coast of North America, serving as critical wintering habitat for diving ducks in the Pacific Flyway. To evaluate the body condition of diving ducks after multiple decades of tidal habitat restoration in SFB, we compared structurally corrected measurements (annual and decadal) of total carcass lipid among canvasback ( Aythya valisineria ), greater scaup ( Aythya marila ), lesser scaup ( Aythya affinis ), and ruddy duck ( Oxyura jamaicensis ). We found greater and lesser scaup gained lipid reserves within the estuary prior to spring migration; however, canvasback and ruddy duck lipid levels declined throughout the winter and into the spring. Lipid loss over the wintering period could indicate a reduction in foraging resource availability, dietary shifts, or metabolic changes. These deficiencies could have negative cross-seasonal implications, as these species must accumulate resources outside of their wintering areas to fuel migration and reproduction. Lipid levels were elevated in lesser scaup collected in 2017-2019 compared to 1998-2000, while we found no decadal differences in lipid levels for canvasback or greater scaup. Our findings suggest tidal restoration in SFB, among other factors, may benefit some diving duck species.

California↗

USGS2001

Seasonal and event-scale variations in solute chemistry for four Sierra Nevada catchments

Hydrobiogeochemical processes controlling stream water chemistry were examined in four small (<5 km2) catchments having contrasting bedrock lithologies in the western Sierra Nevada foothills of California. The Mediterranean climate with its cool/wet and hot/dry cycle produces strong seasonal patterns in hydrological, biological and geochemical processes. Stream water solutes fall into three general groups according to seasonal fluctuation in concentration: Strong, rainy season minimum-dry season maximum (Cl-, SO42-, base cations); weak, rainy season minimum-dry season maximum (Si); and rainy season maximum-dry season minimum (NO3- and K+). Solute dynamics in soil solutions and stream water suggest that mixing of drainage waters from bedrock and soil sources regulate stream water solute concentrations. Patterns are further altered by the leaching of solutes accumulated in the soil over the summer period of desiccation and the temporal discoupling of nutrient cycles that occurs due to differences in the timing between vegetation growth (late spring) and leaching (early winter). Solute concentrations are remarkably similar between watersheds with varying bedrock types, with the exception of nitrate, sulfate and bicarbonate. Three watersheds have nitrogen-bearing metasedimentary bedrock that contributes to elevated nitrate concentrations in stream waters. Watersheds whose bedrock includes mineralized veins of sulfide and carbonate minerals similarly have greater sulfate and bicarbonate concentrations in stream water. Hydrobiogeochemical processes are highly dynamic at the seasonal and storm-event temporal scales and spatially complex at the watershed scale making management of stream water chemical composition, such as nitrate concentrations, very challenging. ?? 2001 Elsevier Science B.V.

Journal of Hydrology↗

USGS2014

Detecting emergence, growth, and senescence of wetland vegetation with polarimetric synthetic aperture radar (SAR) data

Wetlands provide ecosystem goods and services vitally important to humans. Land managers and policymakers working to conserve wetlands require regularly updated information on the statuses of wetlands across the landscape. However, wetlands are challenging to map remotely with high accuracy and consistency. We investigated the use of multitemporal polarimetric synthetic aperture radar (SAR) data acquired with Canada’s Radarsat-2 system to track within-season changes in wetland vegetation and surface water. We speculated, a priori , how temporal and morphological traits of different types of wetland vegetation should respond over a growing season with respect to four energy-scattering mechanisms. We used ground-based monitoring data and other ancillary information to assess the limits and consistency of the SAR data for tracking seasonal changes in wetlands. We found the traits of different types of vertical emergent wetland vegetation were detected well with the SAR data and corresponded with our anticipated backscatter responses. We also found using data from Landsat’s optical/infrared sensors in conjunction with SAR data helped remove confusion of wetland features with upland grasslands. These results suggest SAR data can provide useful monitoring information on the statuses of wetlands over time.

Water↗

USGS2017

Habitat use by juvenile salmonids in Lake Ontario tributaries-species, age, diel and seasonal effects

Understanding the habitat needs of fish and how these requirements may change seasonally over a 24-h period is important, especially for highly managed sport species. Consequently, we examined the diel and seasonal habitat use of four juvenile salmonid species in streams in the Lake Ontario watershed. For juvenile Atlantic salmon Salmo salar and juvenile rainbow trout Oncorhynchus mykiss , differences in day versus night habitat use were more profound than seasonal differences. Observed differences in day versus night habitat for all species and age classes were mainly due to the use of less object oriented cover at night and to a lesser extent to the use of slower velocities and smaller substrate at night. Seasonal differences in habitat use were also observed, likely due to increased fish size, and included movement to deeper and faster water and the use of larger substrate and more cover from summer to winter. Different habitat variables were important to individual species. Juvenile Atlantic salmon were associated with higher water velocities, juvenile rainbow trout with larger substrate and more cover, and subyearling Chinook salmon O. tshawytscha and subyearling coho salmon O. kisutch with small substrate and less cover. Our observations demonstrate that habitat partitioning occurs and likely reduces intraspecific and interspecific competition which may increase the potential production of all four species in sympatry. Consequently, these findings provide important information for resource managers charged with managing, protecting, and enhancing Great Lakes tributaries where all or some of these species occur.

New York↗

USGS2014

Characterizing lentic freshwater fish assemblages using multiple sampling methods

Characterizing fish assemblages in lentic ecosystems is difficult, and multiple sampling methods are almost always necessary to gain reliable estimates of indices such as species richness. However, most research focused on lentic fish sampling methodology has targeted recreationally important species, and little to no information is available regarding the influence of multiple methods and timing (i.e., temporal variation) on characterizing entire fish assemblages. Therefore, six lakes and impoundments (48–1,557 ha surface area) were sampled seasonally with seven gear types to evaluate the combined influence of sampling methods and timing on the number of species and individuals sampled. Probabilities of detection for species indicated strong selectivities and seasonal trends that provide guidance on optimal seasons to use gears when targeting multiple species. The evaluation of species richness and number of individuals sampled using multiple gear combinations demonstrated that appreciable benefits over relatively few gears (e.g., to four) used in optimal seasons were not present. Specifically, over 90 % of the species encountered with all gear types and season combinations ( N  = 19) from six lakes and reservoirs were sampled with nighttime boat electrofishing in the fall and benthic trawling, modified-fyke, and mini-fyke netting during the summer. Our results indicated that the characterization of lentic fish assemblages was highly influenced by the selection of sampling gears and seasons, but did not appear to be influenced by waterbody type (i.e., natural lake, impoundment). The standardization of data collected with multiple methods and seasons to account for bias is imperative to monitoring of lentic ecosystems and will provide researchers with increased reliability in their interpretations and decisions made using information on lentic fish assemblages.

Environmental Monitoring and Assessment↗

USGS2015

Simulating hydrologic response to climate change scenarios in four selected watersheds of New Hampshire

The State of New Hampshire has initiated a coordinated effort to proactively prepare for the effects of climate change on the natural and human resources of New Hampshire. An important aspect of this effort is to develop a vulnerability assessment of hydrologic response to climate change. The U.S. Geological Survey, in cooperation with the New Hampshire Department of Health and Human Services, is developing tools to predict how projected changes in temperature and precipitation will affect change in the hydrology of watersheds in the State. This study is a test case to assemble the information and create the tools to assess the hydrologic vulnerabilities in four specific watersheds. The study uses output from general circulation models to drive hydrologic simulations of streamflow, groundwater base flow (hereafter referred to as base flow), and snowfall in four representative watersheds in New Hampshire during the 21st century, including the watersheds of the Ashuelot, Oyster, Pemigewasset, and Souhegan Rivers. Simulations show that on average, relative to current conditions, streamflow is likely to increase and base flow is likely to decrease, although this change is highly variable by geographic location and season. Streamflow variability will likely increase, with more high streamflows and more low streamflows. The largest increases in streamflow are in the winter, with small decreases in summer. Change in base flow varies across the State with the largest change in the northern Pemigewasset River watershed. Changes in snowfall are consistently decreasing for all watersheds on average, with the largest change also in the Pemigewasset. However, monthly snowfall totals during any given winter could be higher in the future than expected under current conditions. Increasing frequency of floods (the largest seven floods expected to occur in 20 years) could be more significant than the size of the floods, except in the northern high altitude watersheds. In other words, the projections indicate a pattern of multiple floods that might not breach the riverbanks, yet the increased frequency could put additional strain on the existing river banks, infrastructure, and nearby human settlements. There is also likely to be an increase in high flows during the winter and spring months, which could result in more uncertainty in planning for the design, operation, and maintenance of infrastructure, including roads and utilities. Similarly, it is expected that, on average, there will be less base flow available and a wider range of seasonal fluctuation in base flow than experienced historically. These issues could necessitate more attention to planning and management of the resource. Based on past experience, the most important effects of climate change could be less certain planning options and a greater need for planning that accounts for the effects of larger streamflows than are currently available. The effects of hydrologic change on human health and well-being could be most readily apparent with respect to changes in streamflow and the subsequent increase in the frequency of minor flooding and the frequency of summer and fall low streamflows. These changes could require the development of plans to adapt, protect, and upgrade infrastructure, such as bridges, culverts, roads, and other structures. The precipitation runoff modeling shows that rivers and watersheds in New Hampshire will likely change in response to climate change, and that this response varies with season and latitude. Although four representative areas were simulated in this study, additional models could be used to predict the response over the entire State.

New Hampshire↗

USGS2015

Intra-annual patterns in adult band-tailed pigeon survival estimates

Context: The band-tailed pigeon ( Patagioenas fasciata ) is a migratory species occurring in western North America with low recruitment potential and populations that have declined an average of 2.4% per year since the 1960s. Investigations into band-tailed pigeon demographic rates date back to the early 1900s, and existing annual survival rate estimates were derived in the 1970s using band return data. Aims: The primary purpose of the paper was to demonstrate that the apparent paradox between band-tailed pigeon population dynamics (long-term steady decline) and breeding season survival rates (very high) can be explained by changes in survival probability during the remainder of the year. Methods: We trapped Pacific coast band-tailed pigeons during two separate periods: we equipped pigeons with very high frequency (VHF) radio-transmitters in 1999–2000 (1999 = 20; 2000 = 34); and outfitted pigeons with solar powered platform transmitting terminal (PTT) transmitters in 2006–08 (n = 20). We used known fate models to estimate annual survival rates and seasonal survival variation among four periods based on an annual behavioural cycle based on phenological events (nesting, autumn migration, winter and spring migrations). We used model averaged parameter estimates to account for model selection uncertainty. Key results: Neither body condition nor sex were associated with variation in band-tailed pigeon survival rates. Weekly survival during the nesting season did not differ significantly between VHF-marked (0.996; CI = 0.984–0.999) and PTT-marked pigeons (0.998; CI = 0.990–1.00). Model averaged annual survival of PTT-marked pigeons was 0.682 (95% CI = 0.426–0.861) and was similar to annual survival estimated in previous studies using band return data. Survival probability was lowest during both migration periods and highest during the nesting period. Conclusions: Our survival estimates are consistent with those of prior studies and suggest that mortality risk is greatest during migration. Weekly survival probability during winter was nearly the same as during the nesting season; however, winter was the longest period and survival throughout winter was lower than other seasons. Implications: We present the first inter-seasonal analysis of survival probability of the Pacific coast race of band-tailed pigeons and illustrate important temporal patterns that may influence future species management including harvest strategies and disease monitoring.

Wildlife Research↗

USGS2009

Seasonal movements and Home-range use by female pronghorns in sagebrush-steppe communities of western South Dakota

Knowledge of seasonal movements by pronghorns ( Antilocapra americana ) within the easternmost extension of sagebrush-steppe communities is limited. Current hypotheses regarding movement patterns suggest that pronghorns initiate seasonal movements in response to severe winter weather, snowfall patterns, spatial and temporal variation in forage abundance, and availability of water. From January 2002 to August 2005, we monitored movements of 76 adult (≥1.5 years) female pronghorns on 2 study areas (Harding and Fall River counties) in western South Dakota. We collected 8,750 visual locations, calculated 204 home ranges, and documented 17 seasonal movements. Eighty-four percent ( n = 55) of pronghorns were nonmigratory and 10% ( n = 6) were conditional migrators. Mean distance between summer and winter range was 23.1 km ( SE = 2.8 km, n = 13). Five adult pronghorns (8%) dispersed a mean distance of 37.6 km ( SE = 12.4 km); of which 1 female moved a straight-line distance of 75.0 km. Winter and summer home-range size varied ( P < 0.0001) between study sites. Mean 95% adaptive kernel winter and summer home-range size of pronghorns was 55.5 and 19.7 km 2 , respectively, in Harding County and 127.2 and 65.9 km 2 , respectively, in Fall River County. Nonmigratory behavior exhibited by pronghorns was likely associated with minimal snow cover and moderate temperatures during winter 2002–2004. Variation in size of adult seasonal home ranges between sites was likely associated with differences in forage distribution and availability between regions.

Journal of Mammalogy↗

USGS2010

Conserving migratory land birds in the New World: Do we know enough?

Migratory bird needs must be met during four phases of the year: breeding season, fall migration, wintering, and spring migration; thus, management may be needed during all four phases. The bulk of research and management has focused on the breeding season, although several issues remain unsettled, including the spatial extent of habitat influences on fitness and the importance of habitat on the breeding grounds used after breeding. Although detailed investigations have shed light on the ecology and population dynamics of a few avian species, knowledge is sketchy for most species. Replication of comprehensive studies is needed for multiple species across a range of areas. Information deficiencies are even greater during the wintering season, when birds require sites that provide security and food resources needed for survival and developing nutrient reserves for spring migration and, possibly, reproduction. Research is needed on many species simply to identify geographic distributions, wintering sites, habitat use, and basic ecology. Studies are complicated, however, by the mobility of birds and by sexual segregation during winter. Stable-isotope methodology has offered an opportunity to identify linkages between breeding and wintering sites, which facilitates understanding the complete annual cycle of birds. The twice-annual migrations are the poorest-understood events in a bird's life. Migration has always been a risky undertaking, with such anthropogenic features as tall buildings, towers, and wind generators adding to the risk, Species such as woodland specialists migrating through eastern North America have numerous options for pausing during migration to replenish nutrients, but some species depend on limited stopover locations. Research needs for migration include identifying pathways and timetables of migration, quality and distribution of habitats, threats posed by towers and other tall structures, and any bottlenecks for migration. Issues such as human population growth, acid deposition, climate change, and exotic diseases are global concerns with uncertain consequences to migratory birds and even lesscertain remedies. Despite enormous gaps in our understanding of these birds, research, much of it occurring in the past 30 years, has provided sufficient information to make intelligent conservation efforts but needs to expand to handle future challenges. ?? 2010 by the Ecological Society of America.

Ecological Applications↗

USGS2026

Using quantitative polymerase chain reaction to assess phytoplankton and indicate eutrophication in freshwater rivers: A multiyear nationwide study across the United States

Phytoplankton are essential primary producers in fresh surface water that are critical to the health of ecosystems. However, phytoplankton overgrowth due to eutrophication threatens ecological, economic, and public health. Therefore, assessing phytoplankton is fundamental for understanding the productivity, health, and trophic status of freshwater ecosystems. Light microscopy and chlorophyll a assessment are common approaches for studying phytoplankton. They are easy to use, cost-effective, and reliable but have significant limitations. Microscopy has a low throughput and is time-consuming and labor-intensive. Chlorophyll a assessment does not reveal phytoplankton community composition and structure. For comparison, quantitative polymerase chain reaction (qPCR) is widely applied in quantifying microorganisms, offering multiple advantages, including high throughput, sensitivity, accuracy, and robustness. However, a research gap remains regarding the feasibility of using qPCR to assess phytoplankton and indicate trophic status of freshwater bodies. We conducted a nationwide, multiyear study in the United States to compare the performance of qPCR, microscopy, and chlorophyll a assessment in assessing phytoplankton and trophic statuses of multiple freshwater rivers. From early summer to late fall in 2017, 2018, and 2019, we assessed phytoplankton, chlorophyll a , pheophytin a , and the overall Trophic Level Index ( TLI Overall ) at the sampling sites in 12 large freshwater rivers in three regions (western, midcontinent, and eastern) across the United States. The seasonal summed abundance of four major phytoplankton taxa [Bacillariophyta (diatoms), Cyanobacteria (blue-green algae), Chlorophyta (green algae), and Dinoflagellates (Dinophyta)] ranged from 6.88 log 10 (GCN·L –1 ) (the Connecticut River, 2017) to 9.29 log 10 (GCN·L –1 ) (the Kansas River, 2019) (GCN: gene or genome copy number). qPCR- and microscopy-based phytoplankton abundance of eight phytoplankton taxa had a significant positive allometric or log-linear correlation (adjusted R 2 = 0.836, p -value < 0.001, n = 815). In addition, qPCR-based phytoplankton abundance had positive allometric or log-linear correlations with chlorophyll a (adjusted R 2 = 0.5437, p -value < 0.001, n = 164), pheophytin a (adjusted R 2 = 0.3378, p -value < 0.001, n = 164), and TLI Overall (adjusted R 2 = 0.4789, p -value < 0.001, n = 164). Therefore, qPCR is a promising alternative to microscopy and chlorophyll a for studying phytoplankton and trophic status in freshwater rivers. Moreover, phytoplankton abundance had limited temporal variation within each sampling season and over the three sampling seasons in 2017, 2018, and 2019 but showed clear spatial variation. The midcontinent sites had significantly higher phytoplankton abundance, chlorophyll a concentrations, pheophytin a concentrations, and TLI Overall values than those in the eastern and western rivers, reflecting the higher trophic statuses of the midcontinent rivers. This work also provides the thresholds of qPCR-based phytoplankton abundance for delineating trophic statuses in freshwater rivers. Overall, this work demonstrates that qPCR is a promising tool for studying phytoplankton and characterizing the trophic status of freshwater rivers.

Book chapter↗

USGS1998

Colloid particle sizes in the Mississippi River and some of its tributaries, from Minneapolis to below New Orleans

An on-board technique was developed that combined discharge-weighted pumping to a high-speed continuous-flow centrifuge for isolation of the particulate-sized material with ultrafiltration for isolation of colloid-sized material. In order to address whether these processes changed the particle sizes during isolation, samples of particles in suspension were collected at various steps in the isolation process to evaluate changes in particle size. Particle sizes were determined using laser light-scattering photon correlation spectroscopy and indicated no change in size during the colloid isolation process. Mississippi River colloid particle sizes from twelve sites from Minneapolis to below New Orleans were compared with sizes from four tributaries and three seasons, and from predominantly autochthonous sources upstream to more allochthonous sources downstream.

Hydrological Processes↗

USGS1989

Effects of herbage removal on productivity of selected high-Sierra meadow community types

We investigated the effects of herbage removal on three subalpine meadow plant communities in the Rock Creek drainage of Sequoia National Park, California, USA. In the xeric Carex exserta Mkze. (short-hair sedge) type, annual aboveground productivity averaged 19 g/m 2 in control plots (clipped once after plant senescence in late September) over a five-year period. Annual aboveground productivity was enhanced about 30%–35% when plots in this community type were clipped more frequently (i.e., “additional” herbage removal in the early, mid, and late seasons) during each of four treatment years but was reduced by 13%–19% during a fifth (recovery) year in which all but late September clipping was suspended. In a moderately mesic Eleocharis pauciflora (Lightf.) Link. (few-flowered spike rush)- Calamagrostis breweri Thurb. (short-hair grass) type, control plot productivity averaged 115 g/m 2 /yr and was reduced by 20–30% by the additional herbage removal. A more mesic Deschampsia caespitosa (L.) Beauv. (tufted hairgrass)- Carex rostrata Stokes, (beaked sedge) type had the greatest mean above-ground productivity (169 g/m 2 /yr) but also showed damage (i.e., decrease in productivity by 15%–20%) caused by the additional herbage removal. These data suggest that long-term, intensive herbage removal may be more detrimental to moderately mesic and mesic subalpine meadow community types than to xeric types.

California↗

USGS2019

Prediction of ice‐free conditions for a perennially ice‐covered Antarctic lake

Although perennially ice‐covered Antarctic lakes have experienced variable ice thicknesses over the past several decades, future ice thickness trends and associated aquatic biological responses under projected global warming remain unknown. Heat stored in the water column in chemically stratified Antarctic lakes that have middepth temperature maxima can significantly influence the ice thickness trends via upward heat flux to the ice/water interface. We modeled the ice thickness of the west lobe of Lake Bonney, Antarctica, based on possible future climate scenarios utilizing a 1D thermodynamic model that accounts for surface radiative fluxes as well as the heat flux associated with the temperature evolution of the water column. Model results predict that the ice cover of Lake Bonney will shift from perennial to seasonal within one to four decades, a change that will drastically influence ecosystem processes within the lake.

Journal of Geophysical Research F: Earth Surface↗

USGS2004

Habitat use and spatial structure of a barking frog (Eleutherodactylus augusti) population in southeastern Arizona

Barking Frogs ( Eleutherodactylus augusti ) are the northernmost ranging member of the large tropical family Leptodactylidae. We investigated the ecology of this saxicolous species at the northern edge of its range in a canyon in southern Arizona. We captured 54 frogs on discontinuous rock outcrops; eight of nine females and 39 of 45 males were on limestone outcrops. The remaining frogs were closer to limestone outcrops by more than 200 m than would be expected if they were distributed randomly with respect to limestone formations. Seven of 10 frogs radio-tracked had core home ranges (50% fixed kernel) from 94 to 100% on limestone; the other three frogs did not have any part of their home range on limestone outcrops. During five years of mark-recapture efforts, no frogs were found on a different outcrop from the one where they were originally captured; no radio-tracked frogs moved between outcrops during the breeding season. We estimated that four to 20 Barking Frogs occupied each outcrop; these groups probably are connected primarily by juvenile dispersal. As an organism living at the edge of its range, Barking Frogs in Arizona may rely heavily on extensive underground areas such as those found in limestone to protect them from a physiologically challenging environment. To manage for the persistence of Barking Frogs in southern Arizona, we must identify and protect habitat patches and movement pathways among them.

Arizona↗