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At least 1,243 records · Page 69Linked to original sources

Comparison of sample types from white-tailed deer (Odocoileus virginianus) for DNA extraction and analyses

Collection of biological samples for DNA is necessary in a variety of disciplines including disease epidemiology, landscape genetics, and forensics. Quantity and quality of DNA varies depending on the method of collection or media available for collection (e.g., blood, tissue, fecal). Blood is the most common sample collected in vials or on Whatman Flinders Technology Associates (FTA) cards with short- and long-term storage providing adequate DNA for study objectives. The focus of this study was to determine if biological samples stored on Whatman FTA Elute cards were a reasonable alternative to traditional DNA sample collection, storage, and extraction. Tissue, nasal swabs, and ocular fluid were collected from white-tailed deer ( Odocoileus virginianus ). Tissue samples and nasal swabs acted as a control to compare extraction and DNA suitability for microsatellite analysis for nasal swabs and ocular fluid extracted from FTA Elute cards. We determined that FTA Elute cards improved the extraction time and storage of samples and that nasal swabs and ocular fluid containing pigmented fluid were reasonable alternatives to traditional tissue DNA extractions.

Pennsylvania↗

Monitoring abundance of aggregated animals (Florida manatees) using an unmanned aerial system (UAS)

Imperfect detection is an important problem when counting wildlife, but new technologies such as unmanned aerial systems (UAS) can help overcome this obstacle. We used data collected by a UAS and a Bayesian closed capture-mark-recapture model to estimate abundance and distribution while accounting for imperfect detection of aggregated Florida manatees ( Trichechus manatus latirostris ) at thermal refuges to assess use of current and new warmwater sources in winter. Our UAS hovered for 10 min and recorded 4 K video over sites in Collier County, FL. Open-source software was used to create recapture histories for 10- and 6-min time periods. Mean estimates of probability of detection for 1-min intervals at each canal varied by survey and ranged between 0.05 and 0.92. Overall, detection probability for sites varied between 0.62 and 1.00 across surveys and length of video (6 and 10 min). Abundance varied by survey and location, and estimates indicated that distribution changed over time, with use of the novel source of warmwater increasing over time. The highest cumulative estimate occurred in the coldest winter, 2018 (N = 158, CI 141–190). Methods here reduced survey costs, increased safety and obtained rigorous abundance estimates at aggregation sites previously too difficult to monitor.

Florida↗

Multistage time-to-event models improve survival inference by partitioning mortality processes of tracked organisms

Advances in tagging technologies are expanding opportunities to estimate survival of fish and wildlife populations. Yet, capture and handling effects could impact survival outcomes and bias inference about natural mortality processes. We developed a multistage time-to-event model that can partition the survival process into sequential phases that reflect the tagged animal experience, including handling and release mortality, post-release recovery mortality, and subsequently, natural mortality. We demonstrate performance of multistage survival models through simulation testing and through fish and bird telemetry case studies. Models are implemented in a Bayesian framework and can accommodate left, right, and interval censorship events. Our results indicate that accurate survival estimates can be achieved with reasonable sample sizes ( n ≈ 100 + ) "> 𝑛≈100+) and that multimodel inference can inform hypotheses about the configuration and length of survival stages needed to adequately describe mortality processes for tracked specimens. While we focus on survival estimation for tagged fish and wildlife populations, multistage time-to-event models could be used to understand other phenomena of interest such as migration, reproduction, or disease events across a range of taxa including plants and insects.

Scientific Reports↗

Supplying ecosystem services on US rangelands

Rangelands comprise 40% of the conterminous United States and they supply essential ecosystem services to society. A scenario assessment was conducted to determine how accelerating biophysical and societal drivers may modify their future availability. Four scenarios emerged: two may maintain rural communities by sustaining the prevailing ecosystem service of beef cattle production, and two may transform rural communities through expansion of renewable energy technologies and infusion of external capital from amenity land sales. Collaborative organizations representing diverse societal sectors may most effectively identify and manage trade-offs among ecosystem service availability, and equitably prioritize food and energy security, environmental quality and cultural identity.

Nature Sustainability↗

Arsenic and other geogenic contaminants in global groundwater

Geogenic groundwater contaminants (GGCs) affect drinking-water availability and safety, with up to 60% of groundwater sources in some regions contaminated by more than recommended concentrations. As a result, an estimated 300–500 million people are at risk of severe health impacts and premature mortality. In this Review, we discuss the sources, occurrences and cycling of arsenic, fluoride, selenium and uranium, which are GGCs with widespread distribution and/or high toxicity. The global distribution of GGCs is controlled by basin geology and tectonics, with GGC enrichment in both orogenic systems and cratonic basement rocks. This regional distribution is broadly influenced by climate, geomorphology and hydrogeochemical evolution along groundwater flow paths. GGC distribution is locally heterogeneous and affected by in situ lithology, groundwater flow and water–rock interactions. Local biogeochemical cycling also determines GGC concentrations, as arsenic, selenium and uranium mobilizations are strongly redox-dependent. Increasing groundwater extraction and land-use changes are likely to modify GGC distribution and extent, potentially exacerbating human exposure to GGCs, but the net impact of these activities is unknown. Integration of science, policy, community involvement programmes and technological interventions is needed to manage GGC-enriched groundwater and ensure equitable access to clean water.

Nature Reviews Earth & Environment↗

Future water constraints on United States lithium mining under climate change

Lithium is necessary for low-carbon technologies that combat climate change, but lithium extraction is water-intensive. Changes in temperature and precipitation arising from climate change are altering water distribution, which could further strain supplies for new mines and industry, farms, and households. Here we explored how climate change, water use, and mining siting could impact lithium mining in the United States. We analyzed whether there would be sufficient water available to support the single existing and 22 proposed U.S. lithium mines at mid-century under four socioeconomic-climate scenarios and five climate models. Though dependent on socioeconomic-climate scenario, climate model, and lithium deposit type, available water supply in most subbasins would likely be unable to support new mines’ water demands, or even non-mining water demands from other sectors. Water scarcity could hinder the ability of the United States to produce enough lithium to meet domestic demand thereby necessitating higher imports.

conterminous United States↗

Impacts of onshore wind energy production on biodiversity

Wind is increasingly used as a renewable source of energy worldwide. However, harvesting wind energy can have negative consequences for biodiversity. In this Review, we summarize the growth of onshore wind power, its impacts on species and ecosystems, and how those impacts are assessed and mitigated. Across the construction, operation and decommissioning stages, wind facilities are associated with wildlife fatality and behavioural change as well as alteration, loss and fragmentation of terrestrial and aerial habitat. These negative consequences can be mitigated by avoiding construction of wind turbines at sensitive sites, detecting and deterring wildlife, curtailing turbines to reduce fatalities, and replacing lost habitats. Uncertainty about wildlife populations and their demographic parameters, the rate and extent of build-out of onshore wind energy, and best practices for mitigation, as well as variability in regulatory requirements by country or region, all contribute to the difficulty of predicting the consequences of this technology for biodiversity. Scenario-based modelling that incorporates population- and community-level consequences to biodiversity from varying degrees of wind energy development — including the cumulative effects of multiple facilities — is key to addressing this uncertainty.

Nature Biodiversity Reviews↗

An underwater observation dataset for fish classification and fishery assessment

Using Dual-Frequency Identification Sonar (DIDSON), fishery acoustic observation data was collected from the Ocqueoc River, a tributary of Lake Huron in northern Michigan, USA. Data were collected March through July 2013 and 2016 and included the identification, via technology or expert analysis, of eight fish species as they passed through the DIDSON’s field of view. A set of short DIDSON clips containing identified fish was curated. Additionally, two other datasets were created that include visualizations of the acoustic data and longer DIDSON clips. These datasets could complement future research characterizing the abundance and behavior of valued fishes such as walleye ( Sander vitreus ) or white sucker ( Catostomus commersonii ) or invasive fishes such as sea lamprey ( Petromyzon marinus ) or European carp ( Cyprinus carpio ). Given the abundance of DIDSON data and the fact that a portion of it is labeled, these data could aid in the creation of machine learning tools from DIDSON data, particularly for invasive sea lamprey which are amply represented and a destructive invader of the Laurentian Great Lakes.

Scientific Data↗

A portable trap with electric lead catches up to 75% of an invasive fish species

A novel system combining a trap and pulsed direct current electricity was able to catch up to 75% of tagged invasive sea lamprey Petromyzon marinus in free-flowing streams. Non-target mortality was rare and impacts to non-target migration were minimal; likely because pulsed direct current only needed to be activated at night (7 hours of each day). The system was completely portable and the annual cost of the trapping system was low ($4,800 U.S. dollars). Use of the technology is poised to substantially advance integrated control of sea lamprey, which threaten a fishery valued at 7 billion U.S. dollars annually, and help restore sea lamprey populations in Europe where they are native, but imperiled. The system may be broadly applicable to controlling invasive fishes and restoring valued fishes worldwide, thus having far reaching effects on ecosystems and societies.

Scientific Reports↗

Local geology controlled the feasibility of vitrifying Iron Age buildings

During European prehistory, hilltop enclosures made from polydisperse particle-and-block stone walling were exposed to temperatures sufficient to partially melt the constituent stonework, leading to the preservation of glassy walls called ‘vitrified forts’. During vitrification, the granular wall rocks partially melt, sinter viscously and densify, reducing inter-particle porosity. This process is strongly dependent on the solidus temperature, the particle sizes, the temperature-dependence of the viscosity of the evolving liquid phase, as well as the distribution and longevity of heat. Examination of the sintering behaviour of 45 European examples reveals that it is the raw building material that governs the vitrification efficiency. As Iron Age forts were commonly constructed from local stone, we conclude that local geology directly influenced the degree to which buildings were vitrified in the Iron Age. Additionally, we find that vitrification is accompanied by a bulk material strengthening of the aggregates of small sizes, and a partial weakening of larger blocks. We discuss these findings in the context of the debate surrounding the motive of the wall-builders. We conclude that if wall stability by bulk strengthening was the desired effect, then vitrification represents an Iron Age technology that failed to be effective in regions of refractory local geology.

Scientific Reports↗

Introduction: Metallurgical slags - Environmental liability or valuable resource?

Slags are important by-products generated by ferrous and non-ferrous pyrometallurgical operations, with hundreds of millions of tonnes generated globally each year. Depending on the chemical and mineralogical compositions of slags, they may be disposed of as waste, which can then weather and release contaminants into the environment with the potential to impact the ecosystem and humans. Alternately, slags can find use as raw materials with numerous applications in civil engineering or environmental technologies. Furthermore, residual metals, either those targeted for extraction or those that co-occur in the ore or furnace feed and partition into the slag, can be recovered for value. With the ultimate goal of a sustainable environment and a circular economy, the research on slags that is presented in this book will lead us to a better understanding of the environmental consequences of slag disposed of as waste and motivate us to find value in it.

Book chapter↗

Re-evaluation and extension of the scope of elements in US Geological Survey Standard Reference Water Samples

More than 100 US Geological Survey (USGS) Standard Reference Water Samples (SRWSs) were analyzed for numerous trace constituents, including Al, As, B, Ba, Be, Bi, Br, Cd, Cr, Co, Cu, I, Fe, Pb, Li, Mn, Mo, Ni, Rb, Sb, Se, Sr, Te, Tl, U, V, Zn and major elements (Ca, Mg, Na, SiO2, SO4, Cl) by inductively coupled plasma mass spectrometry and inductively coupled plasma atomic emission spectrometry. In addition, 15 USGS SRWSs and National Institute of Standards and Technology (NIST) standard reference material (SRM) 1641b were analyzed for mercury using cold vapor atomic fluorescence spectrometry. Also USGS SRWS Hg-7 was analyzed using isotope dilution-inductively coupled plasma mass spectrometry. The results were compared with the reported certified values of the following standard reference materials: NIST SRM 1643a, 1643b, 1643c and 1643d and National Research Council of Canada Riverine Water Reference Materials for Trace Metals SLRS-1, SLRS-2 and SLRS-3. New concentration values for trace and major elements in the SRWSs, traceable to the certified standards, are reported. Additional concentration values are reported for elements that were neither previously published for the SRWSs nor traceable to the certified reference materials. Robust statistical procedures were used that were insensitive to outliers. These data can be used for quality assurance/quality control purposes in analytical laboratories.

Analyst↗

Resolving global versus local/regional Pu sources in the environment using sector ICP-MS

Sector inductively coupled plasma mass spectrometry is a versatile method for the determination of plutonium activities and isotopic compositions in samples containing this element at fallout levels. Typical detection limits for 239+240Pu are 0.1, 0.02 and 0.002 Bq kg -1Pu for samples sizes of 0.5 g, 3 g, and 50 g of soil, respectively. The application of sector ICP-MS-based Pu determinations is demonstrated in studies in sediment chronology, soil Pu inventory and depth distribution, and the provenance of global fallout versus local or regional Pu sources. A sediment core collected from Sloans Lake (Denver, Colorado, USA) exhibits very similar 137Cs and 239+240Pu activity profiles; 240Pu/239Pu atom ratios indicate possible small influences from the Nevada Test Site and/or the Rocky Flats Environmental Technology Site. An undisturbed soil profile from Lockett Meadow (Flagstaff, Arizona, USA) exhibits an exponential decrease in 239+240Pu activity versus depth; 240Pu/239Pu in the top 3 cm is slightly lower than the global fallout range of 0.180 ?? 0.014 due to possible regional influence of Nevada Test Site fallout. The 239??240Pu inventory at Lockett Meadow is 56 ?? 4 Bq m-2, consistent with Northern Hemisphere mid-latitude fallout. Archived NdF3 sources, prepared from Polish soils, demonstrate that substantial 239+240Pu from the 1986 Chernobyl disaster has been deposited in north eastern regions of Poland; compared to global fallout, Chernobyl Pu exhibits higher abundances of 240Pu and 241Pu. The ratios 240Pu/239pu and 241Pu/239Pu co-vary and range from 0.186-0.348 and 0.0029-0.0412, respectively, in forest soils (241Pu/239Pu = 0.2407??[240Pu/239Pu] - 0.0413; r2 = 0.9924). ?? The Royal Society of Chemistry 2004.

Journal of Analytical Atomic Spectrometry↗

Persistent organic pollutants in Alaskan ringed seal ( Phoca hispida ) and walrus ( Odobenus rosmarus ) blubber

Since 1987, the Alaska Marine Mammal Tissue Archival Project (AMMTAP) has collected tissues from 18 marine mammal species. Specimens are archived in the National Institute of Standards and Technology's National Biomonitoring Specimen Bank (NIST-NBSB). AMMTAP has collected blubber, liver and/or kidney specimens from a number of ringed seals ( Phoca hispida ) from the areas near Nome and Barrow, Alaska and walruses ( Odobenus rosmarus ) from several locations in the Bering Sea. Thirty-three ringed seal and 15 walrus blubber samples from the NIST-NBSB were analyzed for persistent organic pollutants (POPs). The compounds determined included PCBs (28 congeners or congener groups), DDT and related compounds, hexachlorobenzene (HCB), hexachlorocyclohexane isomers (HCHs), chlordanes, dieldrin, and mirex. POP concentrations in ringed seal blubber were significantly higher in Barrow than in Nome when statistically accounting for the interaction of age and gender; HCB, however, was not statistically different between the two locations. Unlike males, POP concentrations and age were not significantly correlated in females probably as a result of lactational loss. POP concentrations in walrus blubber were lower than in ringed seal blubber for ΣPCBs, chlordanes, and HCHs, but higher for dieldrin and mirex. POP concentrations in ringed seals and walrus from Alaska provide further evidence that the western Arctic tends to have lower or similar POP concentrations compared to the eastern Canadian Arctic.

Alaska↗

Development of a standard reference material for Cr(vi) in contaminated soil

Over the last several decades, considerable contamination by hexavalent chromium has resulted from the land disposal of Chromite Ore Processing Residue (COPR). COPR contains a number of hexavalent chromium-bearing compounds that were produced in high temperature industrial processes. Concern over the carcinogenic potential of this chromium species, and its environmental mobility, has resulted in efforts to remediate these waste sites. To provide support to analytical measurements of hexavalent chromium, a candidate National Institute of Standards and Technology (NIST) Standard Reference Material?? (SRM 2701), having a hexavalent chromium content of approximately 500 mg kg -1, has been developed using material collected from a waste site in Hudson County, New Jersey, USA. The collection, processing, preparation and preliminary physico-chemical characterization of the material are discussed. A two-phase multi-laboratory testing study was carried out to provide data on material homogeneity and to assess the stability of the material over the duration of the study. The study was designed to incorporate several United States Environmental Protection Agency (USEPA) determinative methods for hexavalent chromium, including Method 6800 which is based on speciated isotope dilution mass spectrometry (SIDMS), an approach which can account for chromium species inter-conversion during the extraction and measurement sequence. This journal is ?? The Royal Society of Chemistry 2008.

Journal of Analytical Atomic Spectrometry↗

Emerging investigator series: Atmospheric cycling of indium in the northeastern United States

Indium is critical to the global economy and is used in an increasing number of electronics and new energy technologies. However, little is known about its environmental behavior or impacts, including its concentrations or cycling in the atmosphere. This study determined indium concentrations in air particulate matter at five locations across the northeastern United States over the course of one year, in 1995. Historical records from a Massachusetts bog core showed that indium atmospheric concentrations in this region changed only modestly between 1995 and 2010. Atmospheric indium concentrations varied significantly both geographically and temporally, with average concentrations in PM 3 of 2.1 ± 1.6 pg m −3 (1 standard deviation), and average particle-normalized concentrations of 0.2 ± 0.2 μg In per g PM 3 . Peaks in the particle-normalized concentrations in two New York sites were correlated with wind direction; air coming from the north contributed higher concentrations of indium than air coming from the west. This correlation, along with measurements of indium in zinc smelter emissions and coal fly ash, suggests that indium in the atmosphere in the northeastern United States comes from a relatively constant low-level input from coal combustion in the midwest, and higher but more sporadic contributions from the smelting of lead, zinc, copper, tin, and nickel north of the New York sample sites. Understanding the industrial sources of indium to the atmosphere and how they compare with natural sources can lead to a better understanding of the impact of human activities on the indium cycle, and may help to establish a baseline for monitoring future impacts as indium use grows.

Massachusetts, New York↗

Dinucleotide repeat polymorphisms in waterfowl (family Anatidae): Characterization of a sex-linked (Z-specific) and 14 autosomal loci

Canada goose ( Branta Canadensis ) and harlequin duck ( Histrionicus histrionicus ) DNAs were digested with Sau 3AI, and size selected (300-700 bp) fragments were ligated into Bam HI-digested pBluscriptII KS + . The enrichment protocol of Ostrander et al . 1 was followed. The resulting libraries were screened using a [ƴ- 32 P]ATP end-labelled (CA) 20 oligonucleotides as a hybridization probe. Positive clones were sequenced using cycle-sequencing protocols (Epicentre Technologies, Madison, WI) and primers flanking the inserts. PCR primers were designed to amplify the repeat and yield amplification products of ≈100-200 bp. DNA samples were screened for variation at these loci using [ƴ- 32 P]ATP end-labelled primers. The products were resolved using 6% denaturing polyacrylamide gels and autoradiography.

Animal Genetics↗

Conservation endocrinology: A noninvasive tool to understand relationships between carnivore colonization and ecological carrying capacity

Reproductive technology, especially the diagnosis of pregnancy by radioimmunoassay of fecal steroid metabolites, is an important component of captive propagation, but its role in our understanding of ecological interactions and in situ biological restoration has been more limited. Where large herbivores have been 'released' from predation by the extirpation of carnivores, controversy often exists about possible detrimental effects at the ecosystem level A related concern is that the reestablishment of large carnivores may decrease the availability of prey populations for human subsistence. We suggest that pregnancy assays can be a valuable tool to help distinguish between the roles of predation versus food-imposed limitations on population size and their effects on juvenile recruitment in wild species. We explored this issue through analyses of fecal progestagen concentration (FPC) levels to document pregnancy in moose (Alces alces) in the southern Greater Yellowstone Ecosystem, a site where wolves (Canis lupus) and grizzly bears (Ursus arctos) are recolonizing former habitats after an absence of more than 60 years. Pregnancy was clearly discernible (mean FPC for pregnant and nonpregnant females, respectively: 10.60 vs. 2.57 ??g/g; p < 0.0001). Among the potential confounding variables that need to be considered if FPC is applied to ecological and demographic questions are whether baseline values are affected by handling, whether neonate survival has been assessed, and whether sampling efforts are directed at both pregnant and nonpregnant animals. With these issues accounted for, the local moose population experienced juvenile survival rates among the highest in North America. Pregnancy rates, however, dropped from 90% in 1966 to about 75% today, rendering them in the lowest fifteenth percentile among moose populations in North America. Our findings suggest that a relatively low frequency of juvenile moose is not the likely result of predation, and they illustrate how endocrinology can be applied to issues involving reproductive events within an ecological context. They also affirm that noninvasive and generally inexpensive endocrinological procedures will be applicable to understanding interactions between recolonizing predators and prey, an issue that will continue to arise because of global restoration efforts, and to the study of rare ungulates in remote systems where data on reproductive events are difficult to obtain.

Wyoming↗