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At least 1,243 records · Page 69Linked to original sources

Environmental conditions associated with occurrences of the threatened Yaqui Catfish in the Yaqui River Basin, Mexico

The Yaqui Catfish Ictalurus pricei is an understudied species, with limited information available on its ecology, distribution, and local habitat use. Native to the southwestern United States and northwestern Mexico, Yaqui Catfish populations are declining, which has prompted listing of the species as threatened in the United States and as a species of concern in Mexico. Water overallocation, habitat degradation, invasive species introductions, and hybridization with nonnative Channel Catfish I . punctatus have caused the populations in Mexico to decline. The United States population collapsed after years of low recruitment. To better focus conservation efforts as well as define habitat associated with Yaqui Catfish occurrences, we assessed the distribution in the Yaqui River basin of Mexico by using historical data at a landscape scale. Yaqui Catfish were historically found across the watershed among a diversity of environments but were most frequently associated with small, intermittent streams. Basin land cover was dominated by forest, shrubland, and grassland, and Yaqui Catfish generally occurred in stream segments at similar proportions. However, a small number of Yaqui Catfish occurrences were associated with urban and cropland land cover types in proportions greater than the availability of those categories on the landscape. With the species facing declines in the region, this work will help to inform future conservation efforts aimed at securing the Yaqui Catfish, protecting suitable habitat, and better defining its current status in Mexico.

Yaqui River Basin↗

Organohalogenated contaminants in multiple life stages of the Pacific lamprey (Entosphenus tridentatus), Oregon, USA

Pacific lamprey (Entosphenus tridentatus) are ecologically and culturally important anadromous animals native to the West Coast of the United States. Pacific lamprey populations are in decline, and contaminants may be a contributing factor. Between 2017 and 2021, three life stages of Pacific lamprey and collocated sediment samples were collected in Oregon (larval lamprey, sediment, and returning adult lamprey) and off the coast of Oregon and Washington (ocean juvenile lamprey). Tissue and sediment samples were analyzed for 56 organohalogenated compounds that included legacy pesticides, current use pesticides, polybrominated diphenyl ether congeners, and polychlorinated biphenyl congeners. Organohalogenated compounds were detected in all three Pacific lamprey life stages. The organohalogenated compounds detected in collocated sediment and larval lamprey samples were generally dissimilar, and compounds detected in larval lamprey indicate potential point sources along the rivers. Ocean-caught juvenile lamprey had significantly higher lipid contents than returning adult lamprey, but lipid content and concentrations of select compounds were not strongly correlated. Concentrations of select compounds detected in both ocean juvenile and returning adult lamprey were either not significantly different or were higher in returning adult lamprey. Concentrations of some compounds in returning adult lamprey—which are consumed by Indigenous peoples—exceeded state and national human health consumption thresholds. Collaboration among Tribes and public-sector agencies helped make this study successful.

Oregon↗

Ecology of kokanee salmon (Oncorhynchus nerka) and rainbow trout (Oncorhynchus mykiss) in Crater Lake, Oregon

Originally barren of fish, Crater Lake was stocked with approximately 1.8 million salmonids from 1888 to 1941. Rainbow trout ( Oncorhynchus mykiss ) and kokanee salmon ( O. nerka ) now inhabit the lake. This study was conducted from 1986 to 1991 to document and compare kokanee salmon and rainbow trout ecology in the lake to better evaluate the ecological implications of the presence of these non-native fish. Kokanee salmon exhibited cyclic patterns in population age structure, condition, abundance, and biomass from 1986 to 1991. One dominant year class of relatively low abundance and high condition was present from 1986 to 1987. Multiple year classes with increasing abundance and decreasing condition were present from 1989 through 1991. Rainbow trout maintained a diverse population structure throughout the study with a trend toward a relative increase of older age classes and larger fish. Vertical and horizontal migrations of kokanee salmon occurred within and between the nearshore and offshore zones of the lake. Rainbow trout were located along the edge of the lake. Kokanee salmon fed primarily offshore on zooplankton and small-bodied insects. Kokanee salmon cropped the Daphnia population and altered the zooplankton community structure during the study period. Rainbow trout fed nearshore on large-bodied vertebrates and invertebrates. Introduced fish in Crater Lake exhibited the potential to impact limnetic and benthic community structure and nutrient flux within and between these communities.

Oregon↗

Systematic shifts in the variation among host individuals must be considered in climate-disease theory

To make more informed predictions of host–pathogen interactions under climate change, studies have incorporated the thermal performance of host, vector and pathogen traits into disease models to quantify effects on average transmission rates. However, this body of work has omitted the fact that variation in susceptibility among individual hosts affects disease spread and long-term patterns of host population dynamics. Furthermore, and especially for ectothermic host species, variation in susceptibility is likely to be plastic, influenced by variables such as environmental temperature. For example, as host individuals respond idiosyncratically to temperature, this could affect the population-level variation in susceptibility, such that there may be predictable functional relationships between variation in susceptibility and temperature. Quantifying the relationship between temperature and among-host trait variation will therefore be critical for predicting how climate change and disease will interact to influence host–pathogen population dynamics. Here, we use a model to demonstrate how short-term effects of temperature on the distribution of host susceptibility can drive epidemic characteristics, fluctuations in host population sizes and probabilities of host extinction. Our results emphasize that more research is needed in disease ecology and climate biology to understand the mechanisms that shape individual trait variation, not just trait averages.

Proceedings of the Royal Society B: Biological Sci↗

Failure to meet the exchangeability assumption in Bayesian multispecies occupancy models: Implications for study design

Bayesian hierarchical models are ubiquitous in ecology. Random effect model structures are often employed that treat individual effects as deviations from larger population-level effects. In this way individuals are assumed to be "exchangeable" samples. Ecologists may address this exchangeability assumption intuitively, but might in certain modeling contexts ignore it altogether, including in situations where it may have large implications for study design. Multispecies occupancy models based on detection/non-detection data are an approach that can be utilized by those tasked with monitoring rare and endangered species because most literature suggests that, compared to single species occupancy models, improved parameter estimates are assured. Yet, we illustrate through a power analysis how sampling requirements to detect experimental treatment effects vary tremendously depending on whether the species exchangeability assumption is met. The degree to which species in a community respond similarly to covariates governs the ability to accurately estimate parameters using multispecies occupancy models. Detecting small or moderate changes in occupancy resulting from habitat restoration treatments may be impossible for small datasets (e.g., < 36 sampling locations, each surveyed < 8 times) even with a paired treatment-control design if the exchangeability assumption is violated. By contrast, when the assumption is met, small effects may be confidently estimated with as few as 12 sampling locations (6 pairs) and 6-8 survey events. Often, it may be impossible to know whether the exchangeability assumption is met. The statistical power needed to accurately estimate species-specific effects using detection/non-detection multispecies occupancy models depends on the unknown values of treatment effects and whether responses by species in the community diverge. When the species exchangeability assumption is violated, and at lower levels of sampling effort, multispecies occupancy models may provide worse inference than single species occupancy models.

BioRxiv↗

Dynamic social networks based on movement

Network modeling techniques provide a means for quantifying social structure in populations of individuals. Data used to define social connectivity are often expensive to collect and based on case-specific, ad hoc criteria. Moreover, in applications involving animal social networks, collection of these data is often opportunistic and can be invasive. Frequently, the social network of interest for a given population is closely related to the way individuals move. Thus, telemetry data, which are minimally invasive and relatively inexpensive to collect, present an alternative source of information. We develop a framework for using telemetry data to infer social relationships among animals. To achieve this, we propose a Bayesian hierarchical model with an underlying dynamic social network controlling movement of individuals via two mechanisms: an attractive effect and an aligning effect. We demonstrate the model and its ability to accurately identify complex social behavior in simulation, and apply our model to telemetry data arising from killer whales. Using auxiliary information about the study population, we investigate model validity and find the inferred dynamic social network is consistent with killer whale ecology and expert knowledge.

The Annals of Applied Statistics↗

Acoustic telemetry reveals large-scale migration patterns of walleye in Lake Huron

Fish migration in large freshwater lacustrine systems such as the Laurentian Great Lakes is not well understood. The walleye ( Sander vitreus ) is an economically and ecologically important native fish species throughout the Great Lakes. In Lake Huron walleye has recently undergone a population expansion as a result of recovery of the primary stock, stemming from changing food web dynamics. During 2011 and 2012, we used acoustic telemetry to document the timing and spatial scale of walleye migration in Lake Huron and Saginaw Bay. Spawning walleye ( n = 199) collected from a tributary of Saginaw Bay were implanted with acoustic tags and their migrations were documented using acoustic receivers ( n = 140) deployed throughout U.S. nearshore waters of Lake Huron. Three migration pathways were described using multistate mark-recapture models. Models were evaluated using the Akaike Information Criterion. Fish sex did not influence migratory behavior but did affect migration rate and walleye were detected on all acoustic receiver lines. Most (95%) tagged fish migrated downstream from the riverine tagging and release location to Saginaw Bay, and 37% of these fish emigrated from Saginaw Bay into Lake Huron. Remarkably, 8% of walleye that emigrated from Saginaw Bay were detected at the acoustic receiver line located farthest from the release location more than 350 km away. Most (64%) walleye returned to the Saginaw River in 2012, presumably for spawning. Our findings reveal that fish from this stock use virtually the entirety of U.S. nearshore waters of Lake Huron.

Michigan, Ontario↗

Modeling the complex impacts of timber harvests to find optimal management regimes for Amazon tidal floodplain forests

At the Amazon estuary, the oldest logging frontier in the Amazon, no studies have comprehensively explored the potential long-term population and yield consequences of multiple timber harvests over time. Matrix population modeling is one way to simulate long-term impacts of tree harvests, but this approach has often ignored common impacts of tree harvests including incidental damage, changes in post-harvest demography, shifts in the distribution of merchantable trees, and shifts in stand composition. We designed a matrix-based forest management model that incorporates these harvest-related impacts so resulting simulations reflect forest stand dynamics under repeated timber harvests as well as the realities of local smallholder timber management systems. Using a wide range of values for management criteria (e.g., length of cutting cycle, minimum cut diameter), we projected the long-term population dynamics and yields of hundreds of timber management regimes in the Amazon estuary, where small-scale, unmechanized logging is an important economic activity. These results were then compared to find optimal stand-level and species-specific sustainable timber management (STM) regimes using a set of timber yield and population growth indicators. Prospects for STM in Amazonian tidal floodplain forests are better than for many other tropical forests. However, generally high stock recovery rates between harvests are due to the comparatively high projected mean annualized yields from fast-growing species that effectively counterbalance the projected yield declines from other species. For Amazonian tidal floodplain forests, national management guidelines provide neither the highest yields nor the highest sustained population growth for species under management. Our research shows that management guidelines specific to a region&rsquo;s ecological settings can be further refined to consider differences in species demographic responses to repeated harvests. In principle, such fine-tuned management guidelines could make management more attractive, thus bridging the currently prevalent gap between tropical timber management practice and regulation.

Amazon River, Mazagão watershed↗

The bioenergetic consequences of invasive-induced food web disruption to Lake Ontario alewives

Alewives Alosa pseudoharengus are the dominant prey fish in Lake Ontario, and their response to ecological change can alter the structure and function of the Lake Ontario food web. Using stochastic population-based bioenergetic models of Lake Ontario alewives for 1987–1991 and 2001–2005, we evaluated changes to alewife production, consumption, and associated bioenergetic ratios after invasive-induced food web disruption. After the disruption, mean biomass of alewives declined from 28.0 to 14.6 g/m 2 , production declined from 40.8 to 13.6 g·m −2 ·year −1 , and consumption declined from 342.1 to 137.2 g·m −2 ·year −1 , but bootstrapping of error sources suggested that the changes were not statistically significant. Population-based bioenergetic ratios of production to biomass ( P/B ratio), total consumption to biomass ( Q/B ratio), and production efficiency did not change. Pathways of energy flow measured as prey-group-specific Q/B ratios changed significantly between the two time periods for invasive predatory cladocerans (from 0.6 to 1.3), Mysis diluviana (from 0.4 to 2.5), and other prey (from 0.8 to 0.1), but the observed decline in the zooplankton Q/B ratio (from 10.6 to 5.5) was not significant. Gross production efficiency did not change; values ranged from 8% to 15%. Age-group mean gross conversion efficiency (GCE) declined with age; GCE ranged from 7.5% to 11.0% for yearlings, was approximately 5% for age-2 alewives, and was less than 2% for age-3 and older alewives. The GCE increased significantly between the time periods for yearling alewives. Our analyses support the hypothesis that after 2003, alewives could not sustain their growth while feeding on zooplankton closer to shore. Modeling of observed spatial variation in diet and alternative occupied temperatures demonstrates the potential for reducing consumption by alewives. Our results suggest that Lake Ontario alewives can exploit spatial heterogeneity in resource patches and thermal habitat to partially mitigate the effects of food web disruption. Fish management implications are discussed.

North American Journal of Fisheries Management↗

Comparison of longitudinal stream temperature profiles and significant thermal features from airborne thermal infrared and float surveys of the Skykomish, Snoqualmie, and Middle Fork Snoqualmie Rivers, King and Snohomish Counties, Washington, summer 2020 and 2021

Summer water temperatures in the Skykomish, Snoqualmie, and Middle Fork Snoqualmie Rivers in western Washington have in recent decades exceeded the water temperature criteria for aquatic life uses set by the Washington Department of Ecology. This temperature increase is of particular concern because these rivers provide critical habitat for several salmonid populations, including Endangered Species Act-listed Chinook salmon ( Onchorhynchus tshawytscha ), steelhead trout ( O. mykiss ), and bull trout ( Salvelinus confluentus ), thus helping sustain Endangered Species Act-listed Southern Resident orcas ( Orcinus orca ). To inform salmonid restoration efforts within these rivers, this study used high-resolution thermal infrared (TIR) and three-band red, green, blue imagery acquired from repeated airborne surveys conducted in August 2020 and 2021 to (1) quantify longitudinal stream temperature profiles (LTPs) and (2) identify and characterize significant thermal features (STFs), including cold-water anomalies that could represent thermal refuges and serve as salmonid habitat. In addition, drag-probe water temperature surveys (“float surveys”) were performed on the Skykomish and Middle Fork Snoqualmie Rivers during August–September 2020 and on a segment of the Middle Fork Snoqualmie River in August 2021. These float surveys were intended to evaluate this thermal profiling method in comparison to airborne TIR surveys, by employing a novel method of processing float survey data to adjust for diurnal heating. The Middle Fork Snoqualmie River warmed about 7 degrees Celsius (°C) from upstream to downstream in the 2020 airborne TIR survey and 9 °C in the 2021 airborne TIR survey, and the Snoqualmie River warmed about 4 °C in both surveys. The water temperature of the Skykomish River cooled in the 2020 and 2021 surveys, primarily because of cold inflow from the Sultan River. The overall shapes of airborne TIR LTPs of the same river were similar in the 2020 and 2021 surveys, with increasing and decreasing gradients in temperature tending to be nearly parallel over the same reaches and abrupt changes in temperature typically identified at the same locations. A total of 854 STFs were identified in the 2020 TIR imagery, and 732 STFs were identified in the 2021 TIR imagery. Interannual persistence was detected in 36.4 to 61.3 percent of lateral groundwater, side channel, and small tributary STFs, depending on the river surveyed, and in 14.8 to 28.7 percent of hyporheic and diffuse groundwater STFs. Hyporheic flow was commonly detected at the downstream end of a riffle, but not often detected directly downstream from large woody debris. Shade from riparian vegetation did not reduce water temperatures but rather maintained the water temperature recorded just upstream from the shaded section. The adjusted average water temperature profiles from the float surveys were comparable to the LTPs derived from the airborne TIR surveys, with differences in temperature gradient primarily because the surveys were performed under different streamflow, radiation, and shading conditions. Though float surveys were found to be a valuable means of obtaining thermal profiles comparable to profiles obtained by airborne TIR surveys, one key advantage of airborne TIR surveys is that they may be used to precisely locate STFs over long distances, during a short survey duration, and in areas inaccessible to most watercraft.

Washington↗

Distribution and seasonal differences in Pacific Lamprey and Lampetra spp eDNA across 18 Puget Sound watersheds

Lampreys have a worldwide distribution, are functionally important to ecological communities and serve significant roles in many cultures. In Pacific coast drainages of North America, lamprey populations have suffered large declines. However, lamprey population status and trends within many areas of this region are unknown and such information is needed for advancing conservation goals. We developed two quantitative PCR-based, aquatic environmental DNA (eDNA) assays for detection of Pacific Lamprey ( Entosphenus tridentatus ) and Lampetra spp, using locked nucleic acids (LNAs) in the probe design. We used these assays to characterize the spatial distribution of lamprey in 18 watersheds of Puget Sound, Washington, by collecting water samples in spring and fall. Pacific Lamprey and Lampetra spp were each detected in 14 watersheds and co-occurred in 10 watersheds. Lamprey eDNA detection rates were much higher in spring compared to fall. Specifically, the Pacific Lamprey eDNA detection rate was 3.5 times higher in spring and the Lampetra spp eDNA detection rate was 1.5 times higher in spring even though larval lamprey are present in streams year-round. This significant finding highlights the importance of seasonality on eDNA detection. Higher stream discharge in the fall likely contributed to reduced eDNA detection rates, although seasonal life history events may have also contributed. These eDNA assays differentiate Pacific Lamprey and Lampetra spp across much of their range along the west coast of North America. Sequence analysis indicates the Pacific Lamprey assay also targets other Entosphenus spp and indicates the Lampetra spp assay may have limited or no capability of detecting Lampetra in some locations south of the Columbia River Basin. Nevertheless, these assays will serve as a valuable tool for resource managers and have direct application to lamprey conservation efforts, such as mapping species distributions, occupancy modeling, and monitoring translocations and reintroductions.

Puget Sound↗

Ecotoxicology of wild mammals

An international group of 32 scientists has critically reviewed the scientific literature on exposure and effects of environmental contaminants in wild mammals. Although the absolute number of toxicological studies in domesticated and wild mammals eclipses that for birds, a detailed examination of scientific publications and databases reveal that information for 'wild' birds is actually greater than that for 'wild' mammals. Of the various taxa of mammals, ecotoxicological data is most noticeably lacking for marsupials and monotremes. In contrast, rodents (comprising 43% of all mammal species) have been studied extensively, despite evidence of their tolerance to some organochlorine compounds, rodenticides, and even radionuclides. Mammalian species at greatest risk of exposure include those that consume a high percentage of their body weight on a daily basis (e.g., shrews, moles and bats). Aquatic mammals tend to bioaccumulate tremendous burdens of lipophilic contaminants, although storage in their fat depots may actually limit toxicity. Carnivores appear to be more sensitive to adverse effects of environmental contaminants than herbivores. Remarkably few of the thousands of compounds manufactured worldwide have been toxicologically evaluated in wild mammals, and concentrations of even fewer have been monitored in tissues. Overarching research needs include: development of new exposure/effects models and better methods for estimation of species sensitivities; generation of comparative data on contaminant bioavailability, sublethal responses and detoxication mechanisms; enhanced understanding of pesticide, industrial contaminant and metal interactions; identification of endocrine disruptive contaminants and their overall ecological significance; and finally, estimating the relative contribution of environmental contamination as a factor affecting wild mammal populations.

Society of Environmental Toxicology and Chemistry,↗

Spatial capture-recapture

Spatial Capture-Recapture provides a revolutionary extension of traditional capture-recapture methods for studying animal populations using data from live trapping, camera trapping, DNA sampling, acoustic sampling, and related field methods. This book is a conceptual and methodological synthesis of spatial capture-recapture modeling. As a comprehensive how-to manual, this reference contains detailed examples of a wide range of relevant spatial capture-recapture models for inference about population size and spatial and temporal variation in demographic parameters. Practicing field biologists studying animal populations will find this book to be a useful resource, as will graduate students and professionals in ecology, conservation biology, and fisheries and wildlife management.

Book↗

Appendix D: Synthesis element 1 (revised): Water temperature effects on fisheries and stream health in nontidal waters

A limited review of relevant scientific literature related to temperature sensitivities of fish species, stream health indicators, and any related geospatial information was conducted. Based on this review, we provide a syntheses of information related to nontidal waters in the Chesapeake Bay Rising stream temperatures will have a range of impacts on nontidal aquatic ecosystems. Cold headwaters and associated species like brook trout and sculpin are especially vulnerable to higher stream temperatures. Efforts could be taken to identify and protect high quality resilient cold headwater brook trout (Salvelinus fontinalis) habitat. More information on groundwater impacts on stream temperatures and ecologically relevant temperature thresholds for species of concern could help resource managers identify temperature resilient habitats and populations. A vulnerability assessment could be valuable to better understand the drivers and stressors of rising stream temperatures, their effects on aquatic resources, and the risk to fish and other aquatic species. Further research could help in developing and fully vetting a complete list of cold/cool water benthic macroinvertebrate taxa and freshwater mussel taxa that are vulnerable to temperature change in the Chesapeake watershed.

Report↗

Characterizing Golden Eagle risk to lead and anticoagulant rodenticide exposure: A review

Contaminant exposure is among the many threats to Golden Eagle ( Aquila chrysaetos ) populations throughout North America, particularly lead poisoning and anticoagulant rodenticides (AR). These threats may act in concert with others (e.g., lead poisoning and trauma associated with striking objects) to exacerbate risk. Golden Eagles are skilled hunters but also exploit scavenging opportunities, making them particularly susceptible to contaminant exposure from ingesting tissues of poisoned or shot animals. Lead poisoning has long been recognized as an important source of mortality for Golden Eagles throughout North America. More recently, ARs have been associated with both sublethal and lethal effects in raptor species worldwide. In this review, we examine the current state of knowledge for lead and AR exposure in Golden Eagles, drawing from the broader raptor contaminant ecology literature. We examine lead and AR sources within Golden Eagle habitats, exposure routes and toxicity, effects on individuals and populations, synergistic effects, and data and information needs. Continued research addressing data needs and information gaps will help with Golden Eagle conservation planning.

Journal of Raptor Research↗

Metamorphosis in an era of increasing climate variability

Most animals have complex life cycles including metamorphosis or other discrete life stage transitions during which individuals may be particularly vulnerable to environmental stressors. With climate change, individuals will be exposed to increasing thermal and hydrologic variability during metamorphosis, which may affect survival and performance through physiological, behavioral, and ecological mechanisms. Furthermore, because metamorphosis entails changes in traits and vital rates, it is likely to play an important role in how populations respond to increasing climate variability. To identify mechanisms underlying population responses and associated trait and life history evolution, we need new approaches to estimating changes in individual traits and performance throughout metamorphosis, and we need to integrate metamorphosis as an explicit life stage in analytical models.

Trends in Ecology & Evolution↗

Distribution, abundance, and migration timing of Greater and Lesser Sandhill Cranes wintering in the Sacramento-San Joaquin River Delta region of California

The Sacramento-San Joaquin River Delta region of California (hereafter, Delta region) is an important wintering region for the Central Valley Population of greater sandhill cranes ( Grus canadensis tabida ) and lesser sandhill cranes ( G. c. canadensis ), but basic information about the ecology of these birds is lacking to design a biologically sound conservation strategy. During the winters of 2007-08 and 2008-09, we conducted roost counts, roadside surveys, aerial surveys, and tracked radio-marked birds to define the geographic area used by sandhill cranes in the Delta region, document migration chronology, and estimate subspecies-specific abundance. Radio-marked sandhill cranes arrived in our study area beginning 3 October, most arrived in mid-October, and the last radio-marked sandhill crane arrived on 10 December. Departure dates ranged from 15 January to 13 March. Mean arrival and departure dates were similar between subspecies. From mid-December through early-February in 2007-2008, the Delta population ranged from 20,000 to 27,000 sandhill cranes. Abundance varied at the main roost sites during winter because sandhill cranes responded to changes in water conditions. Sandhill cranes used an area of approximately 1,500 km 2 for foraging. Estimated peak abundance in the Delta region was more than half the total number counted on recent Pacific Flyway midwinter surveys, indicating the Delta region is a key area for efforts in conservation and recovery of wintering sandhill cranes in California. Based on arrival dates, flooding of sandhill crane roost sites should be staggered with some sites flooded in early September and most sites flooded by early October. Maintained flooding through mid-March would provide essential roosting habitat until most birds have departed the Delta region on spring migration.

California↗

Polar bear management in Alaska 1997-2000

Since the Twelfth Working Meeting of the IUCN/SSC Polar Bear Specialist Group in 1997, a number of changes in the management of polar bears have occurred in Alaska. On October 16, 2000, the governments of the United States and the Russian Federation signed the “Agreement on the Conservation and Management of the Alaska-Chukotka Polar Bear Population.” This agreement provides substantial benefits for the effective conservation of polar bears shared between the U.S. and Russia. It will require enactment of enabling legislation by the U.S. Congress and other steps by Russia before the agreement has the force of law. A copy of the agreement is included as Appendix 1 to this report. Also, during this period, regulations were developed to implement 1994 amendments to the Marine Mammal Protection Act (MMPA), which allow polar bear trophies taken in approved Canadian populations by U.S. citizens to be imported into the U.S. A summary of the regulatory actions and a table listing populations approved for importation and the number of polar bears imported into the U.S. since 1997 is included in this report. Regarding oil and gas activities in polar bear habitat, three sets of regulations were published authorizing the incidental, non-intentional, taking of small numbers of polar bears concurrent to oil and gas activities. Cooperation continued with the Alaska Nanuuq Commission, representing the polar bear hunting communities in Alaska, as well as with the North Slope Borough and the Inuvialuit Game Council in their agreement for the management of the Southern Beaufort Sea polar bear population. Harvest summaries and technical assistance in designing and assistance in conducting a National Park Service/Alaska Nanuuq Commission study to collect traditional ecological knowledge of polar bear habitat use in Chukotka were provided. In addition, a long-range plan was developed to address and minimize polar bear-human conflicts in North Slope communities. We continued to monitor the harvest of polar bears in Alaska and collect and analyze specimens for presence and level of organochlorine compounds and trace elements. A paper on genetic assessment of hunter reported sex of harvested bears was recently published (Schliebe et al. 1999). Population status and trend assessment efforts continued. An aerial survey of polar bears in the Eastern Chukchi Sea and western portions of the Southern Beaufort Sea was conducted from the U.S. Coast Guard icebreaker “Polar Star” in August 2000. The first year of a multi-year survey of barrier islands and coastlines during the open water and freeze-up phase was conducted in the central Southern Beaufort Sea during fall 2000.

Alaska↗