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Diatom paleoecology Pass Key core 37, Everglades National Park, Florida Bay

During the 20th century, there have been large-scale anthropogenic modifications to the South Florida ecosystem. The effects of these changes on Florida Bay and its biological communities are currently of political and scientific interest. This study is part of a larger effort to reconstruct the history of environmental changes in the bay, using paleoecological techniques. We are using diatom indicators preserved in Florida Bay sediments to infer long-term water quality, productivity, nutrient, and salinity changes. We are also obtaining information concerning the natural variability of the ecosystem. Diatoms are microscopic algae, the remains of which are generally well preserved in sediments, and their distributions are closely linked to water quality. Diatoms were extracted from a 70-cm sediment core collected from the Pass Key mudbank of Florida Bay by the U.S. Geological Survey. Between 300-500 diatom valves from each of 15 core samples were identified and counted. Estimates of absolute abundance, species richness, Shannon-Wiener diversity, and centric:pennate ratios were calculated for each sample that was counted. Information on the ecology of the diatom species is presented, and changes in diatom community composition are evaluated. Samples contained an average of four million diatom valves per gram of sediment. Major changes in the diatom community are evident down core. These include increases in the percent abundance of marine diatoms in the time period represented by the core, probably the result of increasing salinity at Pass Key. Benthic diatoms become less abundant in the top half of the core. This may be related to a number of factors including the die-off of sea grass beds or increased turbidity of the water column. Once the chronology of the Pass Key core 37 is established, these down-core changes can be related to historical events and compared with other indicators in the sedimentary record that are currently being investigated by U.S Geological Survey researchers. This report is preliminary and has not been reviewed for conformity with U.S. Geological Survey editorial standards or with the North American Stratigraphic Code. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

Open-File Report↗

Zooplankton assemblages in montane lakes and ponds of Mount Rainier National Park, Washington State, USA

Water quality and zooplankton samples were collected during the ice-free periods between 1988 and 2005 from 103 oligotrophic montane lakes and ponds located in low forest to alpine vegetation zones in Mount Rainier National Park, Washington State, USA. Collectively, 45 rotifer and 44 crustacean taxa were identified. Most of the numerically dominant taxa appeared to have wide niche breadths. The average number of taxa per lake decreased with elevation and generally increased as maximum lake depths increased (especially for rotifers). With one exception, fish presence/absence did not explain the taxonomic compositions of crustacean zooplankton assemblages. Many rotifer species were common members of zooplankton assemblages in montane lakes and ponds in western North America, whereas the crustacean taxa were common to some areas of the west, but not others. Constraints of the environmental variables did not appear to provide strong gradients to separate the distributions of most zooplankton species. This suggests that interspecific competitive interactions and stochastic processes regulate the taxonomic structures of the zooplankton assemblages at the landscape level. Crustacean species that had broad niche breadths were associated with different rotifer taxa across the environmental gradients. Studies of zooplankton assemblages need to address both crustacean and rotifer taxa, not one or the other.

Journal of Plankton Research↗

An analytical framework for estimating aquatic species density from environmental DNA

Environmental DNA (eDNA) analysis of water samples is on the brink of becoming a standard monitoring method for aquatic species. This method has improved detection rates over conventional survey methods and thus has demonstrated effectiveness for estimation of site occupancy and species distribution. The frontier of eDNA applications, however, is to infer species density. Building upon previous studies, we present and assess a modeling approach that aims at inferring animal density from eDNA. The modeling combines eDNA and animal count data from a subset of sites to estimate species density (and associated uncertainties) at other sites where only eDNA data are available. As a proof of concept, we first perform a cross-validation study using experimental data on carp in mesocosms. In these data, fish densities are known without error, which allows us to test the performance of the method with known data. We then evaluate the model using field data from a study on a stream salamander species to assess the potential of this method to work in natural settings, where density can never be known with absolute certainty. Two alternative distributions (Normal and Negative Binomial) to model variability in eDNA concentration data are assessed. Assessment based on the proof of concept data (carp) revealed that the Negative Binomial model provided much more accurate estimates than the model based on a Normal distribution, likely because eDNA data tend to be overdispersed. Greater imprecision was found when we applied the method to the field data, but the Negative Binomial model still provided useful density estimates. We call for further model development in this direction, as well as further research targeted at sampling design optimization. It will be important to assess these approaches on a broad range of study systems.

Ecology and Evolution↗

Natural source zone depletion of crude oil in the subsurface: Processes controlling mass losses of individual compounds

At many petroleum hydrocarbon spill sites, residual spilled product forms a long-term source of groundwater contamination. The phrase source zone natural depletion is used to refer to the mass loss rates. Overall mass lost under environmental conditions was analyzed using conservative biomarker concentrations for a 1979 oil spill in northern Minnesota, USA. After 40–41 years, an average of 50% of the mass was lost with values ranging from 22% to 57% depending on location. It is also important to understand the composition changes in the source. To understand controls on the losses of individual compounds, concentrations of volatile hydrocarbons in oil samples were compared with aqueous solubilities, and pore-space oil saturations. The results of the comparison show that losses of the oil compounds were controlled by pore-space oil saturations, solubility, and susceptibility to degradation under methanogenic conditions. Compounds that degrade under methanogenic conditions, including toluene, o -xylene, and n -alkanes are more depleted compared to benzene, ethylbenzene, and m - and p -xylene for which losses are dominated by dissolution. These rates and compound-specific behaviors form a foundation for improved modeling approaches and risk analyses.

Minnesota↗

High-speed limnology: Using advanced sensors to investigate spatial variability in biogeochemistry and hydrology

Advanced sensor technology is widely used in aquatic monitoring and research. Most applications focus on temporal variability, whereas spatial variability has been challenging to document. We assess the capability of water chemistry sensors embedded in a high-speed water intake system to document spatial variability. This new sensor platform continuously samples surface water at a range of speeds (0 to >45 km h –1 ) resulting in high-density, mesoscale spatial data. These novel observations reveal previously unknown variability in physical, chemical, and biological factors in streams, rivers, and lakes. By combining multiple sensors into one platform, we were able to detect terrestrial–aquatic hydrologic connections in a small dystrophic lake, to infer the role of main-channel vs backwater nutrient processing in a large river and to detect sharp chemical changes across aquatic ecosystem boundaries in a stream/lake complex. Spatial sensor data were verified in our examples by comparing with standard lab-based measurements of selected variables. Spatial fDOM data showed strong correlation with wet chemistry measurements of DOC, and optical NO 3 concentrations were highly correlated with lab-based measurements. High-frequency spatial data similar to our examples could be used to further understand aquatic biogeochemical fluxes, ecological patterns, and ecosystem processes, and will both inform and benefit from fixed-site data.

Wisconsin↗

U.S. Geological Survey Activities Related to American Indians and Alaska Natives: Fiscal Year 2005

Introduction This report describes the activities that the U.S. Geological Survey (USGS) conducted with American Indian and Alaska Native governments, educational institutions, and individuals during Federal fiscal year (FY) 2005. Most of these USGS activities were collaborations with Tribes, Tribal organizations, or professional societies. Others were conducted cooperatively with the Bureau of Indian Affairs (BIA) or other Federal entities. The USGS is the earth and natural science bureau within the U.S. Department of the Interior (DOI). The USGS does not have regulatory or land management responsibilities. As described in this report, there are many USGS activities that are directly relevant to American Indians, Alaska Natives, and to Native lands. A USGS website, dedicated to making USGS more accessible to American Indians, Alaska Natives, their governments, and institutions, is available at www.usgs.gov/indian. This website includes information on how to contact USGS American Indian/Alaska Native Liaisons, training opportunities, and links to other information resources. This report and previous editions are also available through the website. The USGS realizes that Native knowledge and cultural traditions of living in harmony with nature result in unique Native perspectives that enrich USGS studies. USGS seeks to increase the sensitivity and openness of its scientists to the breadth of Native knowledge, expanding the information on which their research is based. USGS scientific studies include data collection, mapping, natural resource modeling, and research projects. These projects typically last 2 or 3 years, although some are parts of longer-term activities. Some projects are funded cooperatively, with USGS funds matched or supplemented by individual Tribal governments, or by the BIA. These projects may also receive funding from the U.S. Environmental Protection Agency (USEPA), the Indian Health Service (part of the Department of Health and Human Services), or other Federal agencies. The USGS routinely works with its sister bureaus in the Department of the Interior to provide the scientific information and expertise needed to meet the Department's science priorities. Some USGS activities described in this report are conducted as collateral tasks that result from USGS employees identifying and responding to perceived needs. These endeavors are usually prompted by employee interests and frequently involve educational activities. The education is often a reciprocal learning and teaching experience for USGS employees and for Native participants. Through these activities, USGS employees help to fulfill a mission of the USGS - to demonstrate scientific relevance - while helping their fellow citizens. Increasingly, some of the educational activities are becoming parts of formal USGS projects. USGS employees also take initiative in assisting American Indians and Alaska Natives by participating in several organizations that promote awareness of science career opportunities among Native peoples and help build support and communication networks. One such group is the American Indian Science and Engineering Society (AISES). USGS employees join this organization on a voluntary basis, bringing the benefits of this expanded network to the USGS, as many employees do with other professional organizations. The studies briefly described in this report span subsistence issues, wildlife health, water quality, mineral resources, monitoring and modeling to gather information and predict what may happen in the future. Although each project description relates to Native Americans in some way, the projects vary widely, including who conducted the work, the goals and products, the duration of the study, and whether it was local or covered a broad area. Each major organizational unit of the USGS has identified an American Indian/Alaska Native liaison. The USGS has a regional organizational structure, with Western, Central,

Circular↗

Distribution and transport of Olympia oyster, Ostrea lurida, larvae in northern Puget Sound, Washington, USA

As efforts for restoring Olympia oyster (Ostrea lurida) populations have expanded, there is an increased need to understand local factors that could influence the long-term success of these projects. To address concerns over potential limitations to recruitment at a restoration site in northern Puget Sound, Washington, USA, a study was developed to characterize physical processes governing larval transport in conjunction with larval abundance and environmental factors. Larval presence was not associated with tide cycle, season, or a combination of tide cycle and season. In terms of location, larvae were more likely to be present at offshore and intertidal sites versus the estuarine lagoon where the adult population resides. Larval density was higher during late summer ebbs versus early summer floods. Across sampling dates and locations, larval sizes ranged from 184 to 263 µm, indicating that larvae were released into the water column throughout the reproductive season and retained in the embayment for at least ~16 days. Throughout different tidal cycles in Skagit Bay, acoustic Doppler current profilers were used to measure current direction and velocities, concurrent with plankton sampling. Surface currents in the study area alternated between a clockwise and counterclockwise gyre during initial ebb and flood tides, respectively. Larvae exported from the source population during initial to mid-ebbs are swept into a northward gyre, and potentially retained at intertidal sites alongshore. These results will provide resource managers attempting to restore native bivalves with the ability to expand populations by identifying optimal areas for habitat enhancement through natural recruitment.

Washington↗

Heterogeneous detection probabilities for imperiled Missouri River fishes: implications for large-river monitoring programs

Occupancy modeling was used to determine (1) if detection probabilities (p) for 7 regionally imperiled Missouri River fishes (Scaphirhynchus albus, Scaphirhynchus platorynchus, Cycleptus elongatus, Sander canadensis, Macrhybopsis aestivalis, Macrhybopsis gelida, and Macrhybopsis meeki) differed among gear types (i.e. stationary gill nets, drifted trammel nets, and otter trawls), and (2) how detection probabilities were affected by habitat (i.e. pool, bar, and open water), longitudinal position (five 189 to 367 rkm long segments), sampling year (2003 to 2006), and season (July 1 to October 30 and October 31 to June 30). Adult, large-bodied fishes were best detected with gill nets (p: 0.02–0.74), but most juvenile large-bodied and all small-bodied species were best detected with otter trawls (p: 0.02–0.58). Trammel nets may be a redundant sampling gear for imperiled fishes in the lower Missouri River because most species had greater detection probabilities with gill nets or otter trawls. Detection probabilities varied with river segment for S. platorynchus, C. elongatus, and all small-bodied fishes, suggesting that changes in habitat influenced gear efficiency or abundance changes among river segments. Detection probabilities varied by habitat for adult S. albus and S. canadensis, year for juvenile S. albus, C. elongatus, and S. canadensis, and season for adult S. albus. Concentrating sampling effort on gears with the greatest detection probabilities may increase species detections to better monitor a population's response to environmental change and the effects of management actions on large-river fishes.

Endangered Species Research↗

The impact of the 2009-10 El Niño Modoki on U.S. West Coast beaches

High-resolution beach morphology data collected along much of the U.S. West Coast are synthesized to evaluate the coastal impacts of the 2009–10 El Niño. Coastal change observations were collected as part of five beach monitoring programs that span between 5 and 13 years in duration. In California, regional wave and water level data show that the environmental forcing during the 2009–10 winter was similar to the last significant El Niño of 1997–98, producing the largest seasonal shoreline retreat and/or most landward shoreline position since monitoring began. In contrast, the 2009–10 El Niño did not produce anomalously high mean winter-wave energy in the Pacific Northwest (Oregon and Washington), although the highest 5% of the winter wave-energy measurements were comparable to 1997–98 and two significant non-El Niño winters. The increase in extreme waves in the 2009–10 winter was coupled with elevated water levels and a more southerly wave approach than the long-term mean, resulting in greater shoreline retreat than during 1997–98, including anomalously high shoreline retreat immediately north of jetties, tidal inlets, and rocky headlands. The morphodynamic response observed throughout the U.S. West Coast during the 2009–10 El Niño is principally linked to the El Niño Modoki phenomena, where the warm sea surface temperature (SST) anomaly is focused in the central equatorial Pacific (as opposed to the eastern Pacific during a classic El Niño), featuring a more temporally persistent SST anomaly that results in longer periods of elevated wave energy but lower coastal water levels.

Geophysical Research Letters↗

Seasonally dynamic nutrient modeling quantifies storage lags and time-varying reactivity across large river basins

Nutrients that have gradually accumulated in soils, groundwaters, and river sediments in the United States over the past century can remobilize and increase current downstream loading, obscuring effects of conservation practices aimed at protecting water resources. Drivers of storage accumulation and release of nutrients are poorly understood at the spatial scale of basins to watersheds. Predicting water quality outcomes in large river basins demands modeling storage lags and time varying reactivity that models of mean conditions typically cannot elucidate. We developed a seasonally dynamic approach to large-scale nutrient modeling based on a multiscale framework and nutrient storage lags were quantified for the nearly 190 000 small catchments that feed the rivers across the northeastern United States where catchment mean transit times were found to be around 4.7 (2–10) years for nitrogen and 1.3 (0.7–2) years for phosphorus. Nutrient loads carried in river flow in the current season contained a significant—and sometimes dominant—portion of mass lagged in its release from catchment storage repositories. Our approach of integrating storage releases with seasonally dynamic hydroclimatic drivers sets the stage to assess the accumulated effects of nutrient storage and lagged releases to the river interacting with seasonally varying nutrient reactivity and societal management actions throughout large river basins.

Environmental Research Letters↗

Modeling pesticide fate in a small tidal estuary

The exposure analysis modeling system (EXAMS), a pesticide fate model developed by the U.S. Environmental Protection Agency, was modified to model the fate of the herbicides atrazine and metolachlor in a small tidally dominated estuary (Bath Creek) in North Carolina, USA where freshwater inflow accounts for only 3% of the total flow. The modifications simulated the changes that occur during the tidal cycle in the estuary, scenarios that are not possible with the original EXAMS model. Two models were created within EXAMS, a steady-state model and a time-variant tidally driven model. The steady-state model accounted for tidal flushing by simply altering freshwater input to yield an estuary residence time equal to that measured in Bath Creek. The tidal EXAMS model explicitly incorporated tidal flushing by modifying the EXAMS code to allow for temporal changes in estuary physical attributes (e.g., volume). The models were validated with empirical measurements of atrazine and metolachlor concentrations in the estuary shortly after herbicide application in nearby fields and immediately following a rain event. Both models provided excellent agreement with measured concentrations. The steady-state EXAMS model accurately predicted atrazine concentrations in the middle of the estuary over the first 3 days and under-predicted metolachlor by a factor of 2-3. The time-variant, tidally driven EXAMS model accurately predicted the rise and plateau of both herbicides over the 6-day measurement period. We have demonstrated the ability of these modified EXAMS models to be useful in predicting pesticide fate and exposure in small tidal estuaries. This is a significant improvement and expansion of the application of EXAMS, and given the wide use of EXAMS for surface water quality modeling by both researchers and regulators and the ability of EXAMS to interface with terrestrial models (e.g., pesticide root zone model) and bioaccumulation models, we now have an easily-accessible and widely accepted means of modeling chemical fate in estuaries. ?? 2006 Elsevier B.V. All rights reserved.

Ecological Modelling↗

Approaches to setting organism-based ballast water discharge standards

As a vector by which foreign species invade coastal and freshwater waterbodies, ballast water discharge from ships is recognized as a major environmental threat. The International Maritime Organization (IMO) drafted an international treaty establishing ballast water discharge standards based on the number of viable organisms per volume of ballast discharge for different organism size classes. Concerns that the IMO standards are not sufficiently protective have initiated several state and national efforts in the United States to develop more stringent standards. We evaluated seven approaches to establishing discharge standards for the >50-μm size class: (1) expert opinion/management consensus, (2) zero detectable living organisms, (3) natural invasion rates, (4) reaction–diffusion models, (5) population viability analysis (PVA) models, (6) per capita invasion probabilities (PCIP), and (7) experimental studies. Because of the difficulty in synthesizing scientific knowledge in an unbiased and transparent fashion, we recommend the use of quantitative models instead of expert opinion. The actual organism concentration associated with a “zero detectable organisms” standard is defined by the statistical rigor of its monitoring program; thus it is not clear whether such a standard is as stringent as other standards. For several reasons, the natural invasion rate, reaction–diffusion, and experimental approaches are not considered suitable for generating discharge standards. PVA models can be used to predict the likelihood of establishment of introduced species but are limited by a lack of population vital rates for species characteristic of ballast water discharges. Until such rates become available, PVA models are better suited to evaluate relative efficiency of proposed standards rather than predicting probabilities of invasion. The PCIP approach, which is based on historical invasion rates at a regional scale, appears to circumvent many of the indicated problems, although it may underestimate invasions by asexual and parthenogenic species. Further research is needed to better define propagule dose–responses, densities at which Allee effects occur, approaches to predicting the likelihood of invasion from multi-species introductions, and generation of formal comparisons of approaches using standardized scenarios.

Ecological Applications↗

Relating large-scale climate variability to local species abundance: ENSO forcing and shrimp in Breton Sound, Louisiana, USA

Climate creates environmental constraints (filters) that affect the abundance and distribution of species. In estuaries, these constraints often result from variability in water flow properties and environmental conditions (i.e. water flow, salinity, water temperature) and can have significant effects on the abundance and distribution of commercially important nekton species. We investigated links between large-scale climate variability and juvenile brown shrimp Farfantepenaeus aztecus abundance in Breton Sound estuary, Louisiana (USA). Our goals were to (1) determine if a teleconnection exists between local juvenile brown shrimp abundance and the El Niño Southern Oscillation (ENSO) and (2) relate that linkage to environmental constraints that may affect juvenile brown shrimp recruitment to, and survival in, the estuary. Our results identified a teleconnection between winter ENSO conditions and juvenile brown shrimp abundance in Breton Sound estuary the following spring. The physical connection results from the impact of ENSO on winter weather conditions in Breton Sound (air pressure, temperature, and precipitation). Juvenile brown shrimp abundance effects lagged ENSO by 3 mo: lower than average abundances of juvenile brown shrimp were caught in springs following winter El Niño events, and higher than average abundances of brown shrimp were caught in springs following La Niña winters. Salinity was the dominant ENSO-forced environmental filter for juvenile brown shrimp. Spring salinity was cumulatively forced by winter river discharge, winter wind forcing, and spring precipitation. Thus, predicting brown shrimp abundance requires incorporating climate variability into models.

Climate Research↗

Differential response of carbon fluxes to climate in three peatland ecosystems that vary in the presence and stability of permafrost

Changes in vegetation and soil properties following permafrost degradation and thermokarst development in peatlands may cause changes in net carbon storage. To better understand these dynamics, we established three sites in Alaska that vary in permafrost regime, including a black spruce peat plateau forest with stable permafrost, an internal collapse scar bog formed as a result of thermokarst, and a rich fen without permafrost. Measurements include year-round eddy covariance estimates of carbon dioxide (CO 2 ), water, and energy fluxes, associated environmental variables, and methane (CH 4 ) fluxes at the collapse scar bog. The ecosystems all acted as net sinks of CO 2 in 2011 and 2012, when air temperature and precipitation remained near long-term means. In 2013, under a late snowmelt and late leaf out followed by a hot, dry summer, the permafrost forest and collapse scar bog were sources of CO 2 . In this same year, CO 2 uptake in the fen increased, largely because summer inundation from groundwater inputs suppressed ecosystem respiration. CO 2 exchange in the permafrost forest and collapse scar bog was sensitive to warm air temperatures, with 0.5 g C m −2 lost each day when maximum air temperature was very warm (≥29°C). The bog lost 4981 ± 300 mg CH 4 m −2 between April and September 2013, indicating that this ecosystem acted as a significant source of both CO 2 and CH 4 to the atmosphere in 2013. These results suggest that boreal peatland responses to warming and drying, both of which are expected to occur in a changing climate, will depend on permafrost regime.

Alaska↗

Storm-event-transport of urban-use pesticides to streams likely impairs invertebrate assemblages

Insecticide use in urban areas results in the detection of these compounds in streams following stormwater runoff at concentrations likely to cause toxicity for stream invertebrates. In this 2013 study, stormwater runoff and streambed sediments were analyzed for 91 pesticides dissolved in water and 118 pesticides on sediment. Detections included 33 pesticides, including insecticides, fungicides, herbicides, degradates, and a synergist. Patterns in pesticide occurrence reveal transport of dissolved and sediment-bound pesticides, including pyrethroids, from upland areas through stormwater outfalls to receiving streams. Nearly all streams contained at least one insecticide at levels exceeding an aquatic-life benchmark, most often for bifenthrin and (or) fipronil. Multiple U.S. EPA benchmark or criterion exceedances occurred in 40 % of urban streams sampled. Bed sediment concentrations of bifenthrin were highly correlated ( p &thinsp;<&thinsp;0.001) with benthic invertebrate assemblages. Non-insects and tolerant invertebrates such as amphipods, flatworms, nematodes, and oligochaetes dominated streams with relatively high concentrations of bifenthrin in bed sediments, whereas insects, sensitive invertebrates, and mayflies were much more abundant at sites with no or low bifenthrin concentrations. The abundance of sensitive invertebrates, % EPT, and select mayfly taxa were strongly negatively correlated with organic-carbon normalized bifenthrin concentrations in streambed sediments. Our findings from western Clackamas County, Oregon (USA), expand upon previous research demonstrating the transport of pesticides from urban landscapes and linking impaired benthic invertebrate assemblages in urban streams with exposure to pyrethroid insecticides.

Oregon↗

Sediment vulnerability ranking for river basins across the contiguous U.S. using integrated biophysical and social-ecological data

Nearly half of streams and rivers in the United States (U.S.) have fair to poor conditions for aquatic ecosystem health as declared by the U.S. Environmental Protection Agency, and sediment was identified as a primary factor impairing these waterbodies. We rank sediment vulnerability using physical, ecological, and socioeconomic datasets in 163 4-digit Hydrologic Unit Codes within 18 regions across the contiguous U.S. based on the National Hydrography Dataset. We refer to sediment vulnerability as the potential for excess sediment to adversely impact social-ecological aspects of streams and rivers. Metrics were organized into six variable groups: (1) geomorphic setting, (2) modeled sediment and phosphorus, (3) hydrologic conditions, (4) anthropogenic factors and land use, (5) socioeconomic considerations, and (6) measures of ecosystem sensitivity. Correlations and principal component analysis were used to reduce and select metrics that explained the highest variability across basins. Results from the regional analysis identify basins with higher sediment vulnerability in each region, whereas national results identify hotspots of highly ranked basins in the Midwest and the central U.S. with a concentration of moderately to highly ranked basins in the south-central and southwestern U.S. Indicators of socioeconomic and ecological vulnerability integrated with physical drivers of sediment production help identify important gaps in sediment monitoring and research.

contiguous United States↗

Exposure-related effects of Zequanox on juvenile lake sturgeon (Acipenser fulvescens) and lake trout (Salvelinus namaycush)

The environmental fate, persistence, and non-target animal impacts of traditional molluscicides for zebra, Dreissena polymorpha , and quagga, D. bugensis , mussel control led to the development of the biomolluscicide Zequanox. Although previous research has demonstrated the specificity of Zequanox, one study indicated sensitivity of salmonids and lake sturgeon, Acipenser fulvescens , following non-label compliant exposures to Zequanox. This study was conducted to evaluate sublethal and lethal impacts of Zequanox exposure on juvenile lake sturgeon and lake trout, Salvelinus namaycush , following applications that were conducted in a manner consistent with the Zequanox product label. Fish were exposed to 50 or 100 mg/L of Zequanox as active ingredient for 8 h and then held for 33 d to evaluate latent impacts. No acute mortality was observed in either species; however, significant latent mortality (P < 0.01, df = 9; 46.2%) was observed in lake trout that were exposed to the highest dose of Zequanox. Statistically significant (P < 0.03, df = 9), but biologically minimal differences were observed in the weight (range 20.17 to 21.49 g) of surviving lake sturgeon at the termination of the 33 d post-exposure observation period. Statistically significant (P < 0.05, df = 9) and biologically considerable differences were observed in the weight (range 6.19 to 9.55 g) of surviving lake trout at the termination of the 33 d post-exposure observation period. Histologic evaluation of lake trout gastrointestinal tracts suggests that the mode of action in lake trout is different from the mode of action that induces zebra and quagga mussel mortality. Further research could determine the sensitivity of other salmonid species to Zequanox and determine if native fish will avoid Zequanox treated water.

Management of Biological Invasions↗

How quickly do oil and gas wells “Water Out”? Quantifying and contrasting water production trends

Water production from petroleum (oil and natural gas) wells is a topic of increasing environmental and economic importance, yet quantification efforts have been limited to date, and patterns between and within petroleum plays are largely unscrutinized. Additionally, classification of reservoirs as “unconventional” (also known as “continuous”) carries scientific and regulatory importance, but in some cases the distinction from "conventional" wells is unclear. Using water, oil, and gas production data, we calculated a set of quantitative metrics that elucidate trends in the water-to-petroleum ratio over the life of each producing well. The percent growth of the water-to-petroleum ratio quantifies the degree to which a well “waters out” over time; values calculated for 153,900 wells in 18 oil and gas plays show generally much higher values for conventional wells than for continuous/unconventional wells. Analysis of the percent growth along with the slope and median metrics reveals greater variation between conventional plays and between continuous (unconventional) plays than previously recognized. Further, an example from the Bakken Formation in the Williston Basin, USA, illustrates that, within a single play, the metrics provide insight into spatial variation of water production trends, as influenced by geology and reservoir characteristics. By quantifying the variability of water production trends within individual plays and between plays, including differences between conventional and continuous (unconventional) plays, these results provide a more nuanced view of water production from oil and gas wells than has previously been possible and they illustrate the degree to which water management considerations vary spatially and temporally.

Natural Resources Research↗