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Twenty-five essential research questions to inform the protection and restoration of freshwater biodiversity

Freshwater biodiversity is declining at an unprecedented rate. Freshwater conservationists and environmental managers have enough evidence to demonstrate that action must not be delayed but have insufficient evidence to identify those actions that will be most effective in reversing the current trend. Here, the focus is on identifying essential research topics that, if addressed, will contribute directly to restoring freshwater biodiversity through supporting ‘bending the curve’ actions (i.e. those actions leading to the recovery of freshwater biodiversity, not simply deceleration of the current downward trend). The global freshwater research and management community was asked to identify unanswered research questions that could address knowledge gaps and barriers associated with ‘bending the curve’ actions. The resulting list was refined into six themes and 25 questions. Although context-dependent and potentially limited in global reach, six overarching themes were identified: (i) learning from successes and failures; (ii) improving current practices; (iii) balancing resource needs; (iv) rethinking built environments; (v) reforming policy and investments; and (vi) enabling transformative change. Bold, efficient, science-based actions are necessary to reverse biodiversity loss. We believe that conservation actions will be most effective when supported by sound evidence, and that research and action must complement one another. These questions are intended to guide global freshwater researchers and conservation practitioners, identify key projects and signal research needs to funders and governments. Our questions can act as springboards for multidisciplinary and multisectoral collaborations that will improve the management and restoration of freshwater biodiversity.

Aquatic Conservation: Marine and Freshwater Ecosys↗

Evidence for the aquatic binding of arsenate by natural organic matter-suspended Fe(III)

Dialysis experiments with arsenate and three different NOM samples amended with Fe(III) showed evidence confirming the formation of aquatic arsenate-Fe(III)-NOM associations. A linear relationship was observed between the amount of complexed arsenate and the Fe(III) content of the NOM. The dialysis results were consistent with complex formation through ferric iron cations acting as bridges between the negatively charged arsenate and NOM functional groups and/or a more colloidal association, in which the arsenate is bound by suspended Fe(III)-NOM colloids. Sequential filtration experiments confirmed that a significant proportion of the iron present at all Fe/C ratios used in the dialysis experiments was colloidal in nature. These colloids may include larger NOM species that are coagulated by the presence of chelated Fe(III) and/or NOM-stabilized ferric (oxy)hydroxide colloids, and thus, the solution-phase arsenate-Fe(III)-NOM associations are at least partially colloidal in nature. ?? 2006 American Chemical Society.

Environmental Science & Technology↗

Population dynamics of Corbicula fluminea (Müller, 1774) in mesohaline and oligohaline habitats: Invasion success in a Southern Europe estuary

Due to its range expansion and potential ecological effects, Corbicula fluminea is considered one of the most important non-indigenous species (NIS) in aquatic ecosystems. Its presence since 2003 in the upstream area of Mondego estuary (oligohaline and mesohaline sectors) was studied during thirteen months, from December 2007 to December 2008. Monthly mean abundance and biomass ranged from 542 to 11142 individuals m -2 and 13.1–20.4 g Ash Free Dry Weight m -2 , respectively. Populations of C.fluminea were composed mostly of juveniles, always present in extremely high densities compared to other estuarine ecosystems (e.g. Minho estuary) suggesting a continuous recruitment pattern. The hydraulic regime of the River Mondego favours the downstream colonization of the upper Mondego estuary by recruits produced upstream. However, salinity in these sectors of the estuary apparently neither favours growth nor the establishment of structured populations of this species. Other factors like contaminants and predation, which were not studied, could also contribute to the community structure observed.

Estuarine, Coastal and Shelf Science↗

Geographic drivers of mercury entry into aquatic food webs revealed by mercury stable isotopes in dragonfly larvae

Atmospheric mercury (Hg) emissions and subsequent transport and deposition are major concerns within protected lands, including national parks, where Hg can bioaccumulate to levels detrimental to human and wildlife health. Despite this risk to biological resources, there is limited understanding of the relative importance of different Hg sources and delivery pathways within protected regions. Here, we used Hg stable isotope measurements of a single aquatic bioindicator, dragonfly larvae, to determine if these tracers can resolve spatial patterns in Hg sources, delivery mechanisms, and aquatic cycling at a national scale. Mercury isotope values in dragonfly tissues varied among habitat types (e.g., lentic, lotic, wetland) and geographic location. Photochemical-derived isotope fractionation was habitat-dependent and influenced by factors that impact light penetration directly or indirectly, including dissolved organic matter, canopy cover, and total phosphorus. Strong patterns for Δ 200 Hg emerged in the western US, highlighting the relative importance of wet deposition sources in arid regions in contrast to dry deposition delivery in forested regions. This work highlights the efficacy of dragonfly larvae as biosentinels for Hg isotope studies due to their ubiquity across freshwater ecosystems and ability to track variation in Hg sources and processing attributed to small-scale habitat and large-scale regional patterns.

Environmental Science and Technology↗

Metagenomic analysis of planktonic microbial consortia from a non-tidal urban-impacted segment of James River

Knowledge of the diversity and ecological function of the microbial consortia of James River in Virginia, USA, is essential to developing a more complete understanding of the ecology of this model river system. Metagenomic analysis of James River's planktonic microbial community was performed for the first time using an unamplified genomic library and a 16S rDNA amplicon library prepared and sequenced by Ion PGM and MiSeq, respectively. From the 0.46-Gb WGS library (GenBank:SRR1146621; MG-RAST:4532156.3), 4 × 10 6 reads revealed >3 × 10 6 genes, 240 families of prokaryotes, and 155 families of eukaryotes. From the 0.68-Gb 16S library (GenBank:SRR2124995; MG-RAST:4631271.3; EMB:2184), 4 × 10 6 reads revealed 259 families of eubacteria. Results of the WGS and 16S analyses were highly consistent and indicated that more than half of the bacterial sequences were Proteobacteria , predominantly Comamonadaceae . The most numerous genera in this group were Acidovorax (including iron oxidizers, nitrotolulene degraders, and plant pathogens), which accounted for 10 % of assigned bacterial reads. Polaromonas were another 6 % of all bacterial reads, with many assignments to groups capable of degrading polycyclic aromatic hydrocarbons. Albidiferax (iron reducers) and Variovorax (biodegraders of a variety of natural biogenic compounds as well as anthropogenic contaminants such as polycyclic aromatic hydrocarbons and endocrine disruptors) each accounted for an additional 3 % of bacterial reads. Comparison of these data to other publically-available aquatic metagenomes revealed that this stretch of James River is highly similar to the upper Mississippi River, and that these river systems are more similar to aquaculture and sludge ecosystems than they are to lakes or to a pristine section of the upper Amazon River. Taken together, these analyses exposed previously unknown aspects of microbial biodiversity, documented the ecological responses of microbes to urban effects, and revealed the noteworthy presence of 22 human-pathogenic bacterial genera (e.g., Enterobacteriaceae , pathogenic Pseudomonadaceae , and ‘ Vibrionales' ) and 6 pathogenic eukaryotic genera (e.g., Trypanosomatidae and Vahlkampfiidae). This information about pathogen diversity may be used to promote human epidemiological studies, enhance existing water quality monitoring efforts, and increase awareness of the possible health risks associated with recreational use of James River.

Virginia↗

Fisheries research and monitoring activities of the Lake Erie Biological Station, 2025

Lake Erie has the most populated watershed of all the Great Lakes and has undergone dramatic anthropogenic changes. Since the 1800s, overexploitation of fish populations, habitat destruction, non-native species proliferation, industrial contamination, and changes in nutrient loading have impacted the fish community including declines in or extirpation of many native species (Regier et al. 1969, Hartman 1973; Leach & Nepszy 1976; Ludsin et al. 2001). Implementation of the Clean Water Act and Great Lakes Water Quality Agreement in the 1970s improved habitat conditions (Reutter 2019), which contributed to several strong percid year-classes (Vandergoot et al. 2019). These strong year-classes also benefited from more restrictive management practices that reduced harvest, ultimately rehabilitating Lake Erie percid stocks (Kayle et al. 2015, STC 2020). Historically, Lake Erie supported a cool water fish community dominated by percids and salmonids. Recently updated FCOs set forth a vision that “Lake Erie will consist of diverse fish communities that support ongoing societal benefits, including thriving commercial and recreational fisheries, improved fish habitat and desirable ecosystem performance, and reduced adverse impacts from invasive fish” (Francis et al. 2020). Today, mixed fisheries resulting from seasonally changing cool and warm water habitats have developed in Lake Erie, and the new FCOs reflect a desire to manage both predator and prey fish communities within them. Although Lake Erie management agencies have traditionally focused on numerical indices of a few economically important species, aquatic ecosystem models are typically evaluated in terms of entire fish community biomass. As a result, our understanding of fish community structure and ecosystem dynamics from biomass-based models has been limited to short-term investigations and proxy measurements (e.g., length-weight conversion; FTG 2020). Therefore, many Lake Erie fish community databases are now incorporating biomass-based measurements. In response, USGS revised the Lake Erie trawl program to provide biomass-based measurements for all encountered species (Table 1). The survey design change occurred in 2012, coincident with commissioning of a new research vessel and a change in bottom trawl gear. These modifications already altered the existing time series; therefore, the survey design was also expanded to include greater spatial coverage and increased sample size generating a new time series. The purpose of this report is to develop a comprehensive understanding of the long-term changes and fish community dynamics including population dynamics of key fishes of interest to management agencies, such as native percids and their prey. Here, we summarize survey results for the most recent series of West Basin trawl data from 2013 through 2025. Note that a detailed description of the sampling process along with traditional numericallybased catch data (e.g., fish/ha) for individual species can be downloaded online (DuFour et al. 2026) or obtained for earlier years (https://doi.org/10.5066/F75M63X0; U.S. Geological Survey, Great Lakes Science Center 2019).

western Lake Erie↗

Common insecticide disrupts aquatic communities: A mesocosm-to-field ecological risk assessment of fipronil and its degradates in U.S. streams

Insecticides in streams are increasingly a global concern, yet information on safe concentrations for aquatic ecosystems is sparse. In a 30-day mesocosm experiment exposing native benthic aquatic invertebrates to the common insecticide fipronil and four degradates, fipronil compounds caused altered emergence and trophic cascades. Effect concentrations eliciting a 50% response (EC 50 ) were developed for fipronil and its sulfide, sulfone, and desulfinyl degradates; taxa were insensitive to fipronil amide. Hazard concentrations for 5% of affected species derived from up to 15 mesocosm EC 50 values were used to convert fipronil compound concentrations in field samples to the sum of toxic units (∑TU Fipronils ). Mean ∑TU Fipronils exceeded 1 (indicating toxicity) in 16% of streams sampled from five regional studies. The Species at Risk invertebrate metric was negatively associated with ∑TU Fipronils in four of five regions sampled. This ecological risk assessment indicates that low concentrations of fipronil compounds degrade stream communities in multiple regions of the United States.

Science Advances↗

Proceedings of a workshop on the development and evaluation of habitat suitability criteria: A compilation of papers and discussions presented at Colorado State University, Fort Collins, Colorado, December 8-12, 1986

The development of reliable habitat suitability criteria is critical to the successful implementation of the Instream Flow Incremental Methodology (IFIM), or any other habitat based evaluation technology. It is also a fascinating topic of research, for several reasons. First, the “science” of habitat quantification is relatively young. Descriptions of habitat use and partitioning can be traced back to Darwin, if not further. Attempts to actually quantify habitat use can be found predominantly during the last two decades, with most of the activity occurring in about the last five years. Second, this work is challenging because we are usually working with fish or some other organism that lives out of sight in an environment that is foreign to humans. Most of the data collection techniques that have been developed for standard fisheries work are unsuited, without modification, for criteria development. These factors make anyone involved in this type of research a pioneer, of sorts. Pioneers often make new and wonderful discoveries, but they also sometimes get lost. In our opinion, however, there is an even more rewarding aspect to criteria development research. It seems that the field of biology has tended to become increasingly clinical over the years. Criteria development demands the unobtrusive observation of organisms in their natural environment, a fact that allows the biological to be a naturalist and still get paid for it. The relative youth and importance of habitat quantification have resulted in rapid advancements in the state of the art. The expansion of methods is vividly demonstrated simply by comparing the two Instream Flow Information Papers written on the subject in 1978 and in 1986. One of the missions of the Aquatic Systems Branch (formerly the Instream Flow Group) is to serve as a clearinghouse for new techniques and methods. In keeping with this role, a workshop was conducted during December 1986 to discuss current and newly evolving methods for developing and evaluating habitat suitability criteria. Participation in this workshop was largely by invitation only. The objective was to obtain insights into problems and possible solutions to criteria development, from the perspective of professionals closely involved with the subject. These proceedings of that workshop are intended to supplement the information contained in Instream Flow Information Paper 21, "Development and Evaluation of Habitat Suitability Criteria for Use in the Instream Flow Incremental Methodology." The workshop was closely arranged in five sessions, roughly following the outline of Information Paper 21. The first session dealt with various aspects of study design and how they can influence the outcome of a study. Session two investigated techniques for developing criteria from professional judgment, and some of the problems encountered when personal or agency prejudice enters the picture. Session three concentrated on field data collection procedures, whereas session four examined methods of converting field data into curves. Field verification studies were discussed in session five. Each presentation in the workshop was followed by a question and answer period of 15 to 30 minutes. These discussions were recorded, transcribed, and appended to the end of each paper in these proceedings. We have attempted to capture the essence of these discussions as accurately as possible, but hope that the reader can appreciate the difficulty in translating a free-ranging discussion (from a barely audible tape) to something that makes sense in print. These question and answer sessions constitute the peer review for each of the papers. This provides the reader with the unique opportunity to review the interactions between authors and reviewers.

Book↗

Nutrients mediate the effects of temperature on methylmercury concentrations in freshwater zooplankton

Methylmercury (MeHg) bioaccumulation in freshwater aquatic systems is impacted by anthropogenic stressors, including climate change and nutrient enrichment. The goal of this study was to determine how warmer water temperatures and excess nutrients would alter zooplankton communities and phytoplankton concentrations, and whether those changes would in turn increase or decrease MeHg concentrations in freshwater zooplankton. To test this, we employed a 2x2 factorial experimental design with nutrient and temperature treatments. Mesocosms were filled with ambient water and plankton from Cottage Grove Reservoir, Oregon, U.S.A, a waterbody that has experienced decades of elevated MeHg concentrations and corresponding fish consumption advisories due to run-off from Black Butte Mine tailings, located within the watershed. Treatment combinations of warmer temperature (increased by 0.7°C) and nutrient addition (a single pulse of 10 ambient concentrations of nitrogen and phosphorous), control, and a combination of temperature and nutrients were applied to mesocosms.

Oregon↗

Influence of sediment chemistry and sediment toxicity on macroinvertebrate communities across 99 wadable streams of the Midwestern USA

Simultaneous assessment of sediment chemistry, sediment toxicity, and macroinvertebrate communities can provide multiple lines of evidence when investigating relations between sediment contaminants and ecological degradation. These three measures were evaluated at 99 wadable stream sites across 11 states in the Midwestern United States during the summer of 2013 to assess sediment pollution across a large agricultural landscape. This evaluation considers an extensive suite of sediment chemistry totaling 274 analytes (polycyclic aromatic hydrocarbons, organochlorine compounds, polychlorinated biphenyls, polybrominated diphenyl ethers, trace elements, and current-use pesticides) and a mixture assessment based on the ratios of detected compounds to available effects-based benchmarks. The sediments were tested for toxicity with the amphipod Hyalella azteca (28-d exposure), the midge Chironomus dilutus (10-d), and, at a few sites, with the freshwater mussel Lampsilis siliquoidea (28-d). Sediment concentrations, normalized to organic carbon content, infrequently exceeded benchmarks for aquatic health, which was generally consistent with low rates of observed toxicity. However, the benchmark-based mixture score and the pyrethroid insecticide bifenthrin were significantly related to observed sediment toxicity. The sediment mixture score and bifenthrin were also significant predictors of the upper limits of several univariate measures of the macroinvertebrate community (EPT percent, MMI (Macroinvertebrate Multimetric Index) Score, Ephemeroptera and Trichoptera richness) using quantile regression. Multivariate pattern matching (Mantel-like tests) of macroinvertebrate species per site to identified contaminant metrics and sediment toxicity also indicate that the sediment mixture score and bifenthrin have weak, albeit significant, influence on the observed invertebrate community composition. Together, these three lines of evidence (toxicity tests, univariate metrics, and multivariate community analysis) suggest that elevated contaminant concentrations in sediments, in particular bifenthrin, is limiting macroinvertebrate communities in several of these Midwest streams.

Science of the Total Environment↗

An introduction to standardized sampling

In 2009, the first edition of Standard Methods for Sampling North American Freshwater Fishes was published. This was the first time in the history of fisheries science that standardization of methods and equipment had taken place on such a large geographic scale. Since its publication, the methods have been used extensively across North America by local, state, and federal agencies, organizations, and universities who have seen the advantages of large-scale data comparison. Authors have been invited to present these methods in other locations around the world to help with standard sampling programs on other continents. Now, with large-scale issues such as human-caused climate change, effects of landscape-scale regulations, and effects of habitat degradation continuing to increase in importance, the ability to compare data across wide regions and political boundaries, compare data over time, and collect data with improved accuracy and precision is more important than ever. This new edition of Standard Methods is sponsored by the American Fisheries Society (AFS), the U.S. Fish and Wildlife Service, and the Association of Fish and Wildlife Agencies (AFWA), with contributions by numerous state, provincial and federal agencies, and numerous academic institutions and nongovernmental organizations (NGOs). It is authored by over 100 experts in fisheries sampling from across Canada, Mexico, and the United States. Most techniques for water body types addressed in the first edition have been kept the same-in the interest of standardization over time; however, many important additions have been made. Like the first edition, these methods are designed for fish community assessments in North American aquatic systems (e.g., for this edition, lakes, ponds, rivers, and streams containing warmwater and coldwater species; the Great Lakes, wetlands, and cenotes). Although other methods may be available that better target a more specific size-group or species, these techniques were selected as most effective for general surveys of these systems and typically are the most effective for capturing the common fishes found in these waters.

Book chapter↗

Simulating the effect of climate change on stream temperature in the Trout Lake Watershed, Wisconsin

The potential for increases in stream temperature across many spatial and temporal scales as a result of climate change can pose a difficult challenge for environmental managers, especially when addressing thermal requirements for sensitive aquatic species. This study evaluates simulated changes to the thermal regime of three northern Wisconsin streams in response to a projected changing climate using a modeling framework and considers implications of thermal stresses to the fish community. The Stream Network Temperature Model (SNTEMP) was used in combination with a coupled groundwater and surface water flow model to assess forecasts in climate from six global circulation models and three emission scenarios. Model results suggest that annual average stream temperature will steadily increase approximately 1.1 to 3.2 °C (varying by stream) by the year 2100 with differences in magnitude between emission scenarios. Daily mean stream temperature during the months of July and August, a period when cold-water fish communities are most sensitive, showed excursions from optimal temperatures with increased frequency compared to current conditions. Projections of daily mean stream temperature, in some cases, were no longer in the range necessary to sustain a cold water fishery.

Wisconsin↗

Ecological risks of shale oil and gas development to wildlife, aquatic resources and their habitats

Technological advances in hydraulic fracturing and horizontal drilling have led to the exploration and exploitation of shale oil and gas both nationally and internationally. Extensive development of shale resources has occurred within the United States over the past decade, yet full build out is not expected to occur for years. Moreover, countries across the globe have large shale resources and are beginning to explore extraction of these resources. Extraction of shale resources is a multistep process that includes site identification, well pad and infrastructure development, well drilling, high-volume hydraulic fracturing and production; each with its own propensity to affect associated ecosystems. Some potential effects, for example from well pad, road and pipeline development, will likely be similar to other anthropogenic activities like conventional gas drilling, land clearing, exurban and agricultural development and surface mining (e.g., habitat fragmentation and sedimentation). Therefore, we can use the large body of literature available on the ecological effects of these activities to estimate potential effects from shale development on nearby ecosystems. However, other effects, such as accidental release of wastewaters, are novel to the shale gas extraction process making it harder to predict potential outcomes. Here, we review current knowledge of the effects of high-volume hydraulic fracturing coupled with horizontal drilling on terrestrial and aquatic ecosystems in the contiguous United States, an area that includes 20 shale plays many of which have experienced extensive development over the past decade. We conclude that species and habitats most at risk are ones where there is an extensive overlap between a species range or habitat type and one of the shale plays (leading to high vulnerability) coupled with intrinsic characteristics such as limited range, small population size, specialized habitat requirements, and high sensitivity to disturbance. Examples include core forest habitat and forest specialists, sagebrush habitat and specialists, vernal pond inhabitants and stream biota. We suggest five general areas of research and monitoring that could aid in development of effective guidelines and policies to minimize negative impacts and protect vulnerable species and ecosystems: (1) spatial analyses, (2) species-based modeling, (3) vulnerability assessments, (4) ecoregional assessments, and (5) threshold and toxicity evaluations.

Environmental Science & Technology↗

Spatial and temporal trends in occurrence of emerging and legacy contaminants in the Lower Columbia River 2008-2010

The Lower Columbia River in Oregon and Washington, USA, is an important resource for aquatic and terrestrial organisms, agriculture, and commerce. An 86-mile stretch of the river was sampled over a 3 year period in order to determine the spatial and temporal trends in the occurrence and concentration of water-borne organic contaminants. Sampling occurred at 10 sites along this stretch and at 1 site on the Willamette River using the semipermeable membrane device (SPMD) and the polar organic chemical integrative sampler (POCIS) passive samplers. Contaminant profiles followed the predicted trends of lower numbers of detections and associated concentrations in the rural areas to higher numbers and concentrations at the more urbanized sites. Industrial chemicals, plasticizers, and PAHs were present at the highest concentrations. Differences in concentrations between sampling periods were related to the amount of rainfall during the sampling period. In general, water concentrations of wastewater-related contaminants decreased and concentrations of legacy contaminants slightly increased with increasing rainfall amounts.

Oregon;Washington↗

A dynamic water-quality modeling framework for the Neuse River estuary, North Carolina

As a result of fish kills in the Neuse River estuary in 1995, nutrient reduction strategies were developed for point and nonpoint sources in the basin. However, because of the interannual variability in the natural system and the resulting complex hydrologic-nutrient inter- actions, it is difficult to detect through a short-term observational program the effects of management activities on Neuse River estuary water quality and aquatic health. A properly constructed water-quality model can be used to evaluate some of the potential effects of manage- ment actions on estuarine water quality. Such a model can be used to predict estuarine response to present and proposed nutrient strategies under the same set of meteorological and hydrologic conditions, thus removing the vagaries of weather and streamflow from the analysis. A two-dimensional, laterally averaged hydrodynamic and water-quality modeling framework was developed for the Neuse River estuary by using previously collected data. Development of the modeling framework consisted of (1) computational grid development, (2) assembly of data for model boundary conditions and model testing, (3) selection of initial values of model parameters, and (4) limited model testing. The model domain extends from Streets Ferry to Oriental, N.C., includes seven lateral embayments that have continual exchange with the main- stem of the estuary, three point-source discharges, and three tributary streams. Thirty-five computational segments represent the mainstem of the estuary, and the entire framework contains a total of 60 computa- tional segments. Each computational cell is 0.5 meter thick; segment lengths range from 500 meters to 7,125 meters. Data that were used to develop the modeling framework were collected during March through October 1991 and represent the most comprehensive data set available prior to 1997. Most of the data were collected by the North Carolina Division of Water Quality, the University of North Carolina Institute of Marine Sciences, and the U.S. Geological Survey. Limitations in the modeling framework were clearly identified. These limitations formed the basis for a set of suggestions to refine the Neuse River estuary water-quality model.

North Carolina↗

Accumulation of contaminants in fish from wastewater treatment wetlands

Increasing demands on water resources in arid environments make reclamation and reuse of municipal wastewater an important component of the water budget. Treatment wetlands can be an integral part of the water-reuse cycle providing both water-quality enhancement and habitat functions. When used for habitat, the bioaccumulation potential of contaminants in the wastewater is a critical consideration. Water and fish samples collected from the Tres Rios Demonstration Constructed Wetlands near Phoenix, Arizona, which uses secondary-treated wastewater to maintain an aquatic ecosystem in a desert environment, were analyzed for hydrophobic organic compounds (HOC) and trace elements. Semipermeable membrane devices (SPMD) were deployed to investigate uptake of HOC. The wetlands effectively removed HOC, and concentrations of herbicides, pesticides, and organic wastewater contaminants decreased 40−99% between inlet and outlet. Analysis of Tilapia mossambica and Gambusia affinis indicated accumulation of HOC, including p,p ‘-DDE and trans -nonachlor. The SPMD accumulated the HOC detected in the fish tissue as well as additional compounds. Trace-element concentrations in whole-fish tissue were highly variable, but were similar between the two species. Concentrations of HOC and trace elements varied in different fish tissue compartments, and concentrations in Tilapia liver tissue were greater than those in the whole organism or filet tissue. Bioconcentration factors for the trace elements ranged from 5 to 58 000 and for the HOC ranged from 530 to 150 000.

Environmental Science & Technology↗

Concentrations of cadmium, lead, and zinc in fish from mining-influenced waters of northeastern Oklahoma: Sampling of blood, carcass, and liver for aquatic biomonitoring

The Tri-States Mining District (TSMD) of Missouri (MO), Kansas (KS), and Oklahoma (OK), USA, was mined for lead (Pb) and zinc (Zn) for more than a century. Mining ceased more than 30 years ago, but wastes remain widely distributed in the region, and there is evidence of surface- and groundwater contamination in the Spring River-Neosho River (SR-NR) system of northeastern OK. In October 2001, we collected a total of 74 fish from six locations in the SR-NR system that included common carp (Cyprinus carpio), channel- and flathead catfish (Ictalurus punctatus and Pylodictis olivaris), largemouth- and spotted bass (Micropterus salmoides and Micropterus punctulatus), and white crappie (Pomoxis annularis). We obtained additional fish from locations in MO that included three reference sites and one site that served as a "positive control" (heavily contaminated by Pb). Blood, carcass (headed, eviscerated, and scaled) and liver (carp only) samples were analyzed for cadmium (Cd), Pb, and Zn. Our objectives were to assess the degree to which fish from the OK portion of the SR-NR system are contaminated by these elements and to evaluate fish blood sampling for biomonitoring. Concentrations of Cd and Pb in carp and catfish from OK sites were elevated and Pb concentrations of some approached those of the highly contaminated site in MO, but concentrations in bass and crappie were relatively low. For Zn, correlations were weak among concentrations in the three tissues and none of the samples appeared to reflect site contamination. Variability was high for Cd in all three tissues of carp; differences between sites were statistically significant (p < 0.05) only for blood even though mean liver concentrations were at least 100-fold greater than those in blood. Blood concentrations of Cd and Pb were positively correlated (r 2 = 0.49 to 0.84) with the concentration of the same element in carp and catfish carcasses or in carp livers, and the corresponding multiple regression models were highly significant (p < 0.001). Our data indicate that potentially nonlethal blood sampling can be useful for monitoring of selected metals in carp, catfish, and perhaps other fishes. ?? 2005 Springer Science+Business Media, Inc.

Archives of Environmental Contamination and Toxico↗

Development and Evaluation of a Riparian Buffer Mapping Tool

Land use and land cover within riparian areas greatly affect the conditions of adjacent water features. In particular, riparian forests provide many environmental benefits, including nutrient uptake, bank stabilization, steam shading, sediment trapping, aquatic and terrestrial habitat, and stream organic matter. In contrast, residential and commercial development and associated transportation infrastructure increase pollutant and nutrient loading and change the hydrologic characteristics of the landscape, thereby affecting both water quality and habitat. Restoring riparian areas is a popular and cost effective restoration technique to improve and protect water quality. Recognizing this, the Chesapeake Executive Council committed to restoring 10,000 miles of riparian forest buffers throughout the Chesapeake Bay watershed by the year 2010. In 2006, the Chesapeake Executive Council further committed to 'using the best available...tools to identify areas where retention and expansion of forests is most needed to protect water quality'. The Chesapeake Bay watershed encompasses 64,000 square miles, including portions of six States and Washington, D.C. Therefore, the interpretation of remotely sensed imagery provides the only effective technique for comprehensively evaluating riparian forest protection and restoration opportunities throughout the watershed. Although 30-meter-resolution land use and land cover data have proved useful on a regional scale, they have not been equally successful at providing the detail required for local-scale assessment of riparian area characteristics. Use of high-resolution imagery (HRI) provides sufficient detail for local-scale assessments, although at greater cost owing to the cost of the imagery and the skill and time required to process the data. To facilitate the use of HRI for monitoring the extent of riparian forest buffers, the U.S. Forest Service and the U.S. Geological Survey Eastern Geographic Science Center funded the development of a prototype semiautomated image classification tool, RBMapper, that is designed for use by technicians with limited image processing training. This document provides an overview of the RBMapper tool, includes instructions on how to obtain the RBMapper tool and tutorial datasets, and contains a summary evaluation of the tool

Open-File Report↗