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Principles and Practices for Quality Assurance and Quality Control

Quality assurance and quality control are vital parts of highway runoff water-quality monitoring projects. To be effective, project quality assurance must address all aspects of the project, including project management responsibilities and resources, data quality objectives, sampling and analysis plans, data-collection protocols, data quality-control plans, data-assessment procedures and requirements, and project outputs. Quality control ensures that the data quality objectives are achieved as planned. The historical development and current state of the art of quality assurance and quality control concepts described in this report can be applied to evaluation of data from prior projects.

Open-File Report↗

Field-based evaluation of two herbaceous plant community composition sampling methods for long-term monitoring in Northern Great Plains National Parks

The Northern Great Plains Inventory & Monitoring (I&M) Network (Network) of the National Park Service (NPS) consists of 13 NPS units in North Dakota, South Dakota, Nebraska, and eastern Wyoming. The Network is in the planning phase of a long-term program to monitor the health of park ecosystems. Plant community composition is one of the 'Vital Signs,' or indicators, that will be monitored as part of this program for three main reasons. First, plant community composition is information-rich; a single sampling protocol can provide information on the diversity of native and non-native species, the abundance of individual dominant species, and the abundance of groups of plants. Second, plant community composition is of specific management concern. The abundance and diversity of exotic plants, both absolute and relative to native species, is one of the greatest management concerns in almost all Network parks (Symstad 2004). Finally, plant community composition reflects the effects of a variety of current or anticipated stressors on ecosystem health in the Network parks including invasive exotic plants, large ungulate grazing, lack of fire in a fire-adapted system, chemical exotic plant control, nitrogen deposition, increased atmospheric carbon dioxide concentrations, and climate change. Before the Network begins its Vital Signs monitoring, a detailed plan describing specific protocols used for each of the Vital Signs must go through rigorous development and review. The pilot study on which we report here is one of the components of this protocol development. The goal of the work we report on here was to determine a specific method to use for monitoring plant community composition of the herb layer (< 2 m tall).

Open-File Report↗

Monitoring multiple species: Estimating state variables and exploring the efficacy of a monitoring program

Monitoring programs have the potential to identify population declines and differentiate among the possible cause(s) of these declines. Recent criticisms regarding the design of monitoring programs have highlighted a failure to clearly state objectives and to address detectability and spatial sampling issues. Here, we incorporate these criticisms to design an efficient monitoring program whose goals are to determine environmental factors which influence the current distribution and measure change in distributions over time for a suite of amphibians. In designing the study we (1) specified a priori factors that may relate to occupancy, extinction, and colonization probabilities and (2) used the data collected (incorporating detectability) to address our scientific questions and adjust our sampling protocols. Our results highlight the role of wetland hydroperiod and other local covariates in the probability of amphibian occupancy. There was a change in overall occupancy probabilities for most species over the first three years of monitoring. Most colonization and extinction estimates were constant over time (years) and space (among wetlands), with one notable exception: local extinction probabilities for Rana clamitans were lower for wetlands with longer hydroperiods. We used information from the target system to generate scenarios of population change and gauge the ability of the current sampling to meet monitoring goals. Our results highlight the limitations of the current sampling design, emphasizing the need for long-term efforts, with periodic re-evaluation of the program in a framework that can inform management decisions.

Biological Conservation↗

The potential uses of operational earthquake forecasting

This article reports on a workshop held to explore the potential uses of operational earthquake forecasting (OEF). We discuss the current status of OEF in the United States and elsewhere, the types of products that could be generated, the various potential users and uses of OEF, and the need for carefully crafted communication protocols. Although operationalization challenges remain, there was clear consensus among the stakeholders at the workshop that OEF could be useful.

Seismological Research Letters↗

Development of a domestic earthquake alert protocol combining the USGS pager and FEMA Hazus systems

The U.S. Geological Survey’s PAGER automated alert system provides rapid (10-20 min) loss estimates in terms of ranges of fatalities and economic impact for all significant earthquakes around the globe. In contrast, FEMA’s Hazus software, which is currently operated manually by FEMA personnel internally within several hours of any large domestic earthquake, provides more detailed loss information quantified in terms of physical damage to the building stock, as well as a broad range of social and economic consequences estimated at a much higher spatial resolution (census-tract level). Hazus was originally designed for FEMA’s mitigation planning, but has been used of late for post-earthquake situational awareness. While the quick alerts generated automatically by the PAGER system are a suitable way to initially identify consequences for earthquakes, there remains a need to provide detailed loss information for facilitating a wider array of post-earthquake decisions among emergency managers. As such, we have developed a prototype summary product that takes advantage of the benefits of both these loss models for significant domestic earthquakes. The signature product, a new twoPAGER report, will serve as a supplement to the widely deployed standard onePAGER product for all significant domestic earthquakes. Page one is the standard, automated PAGER alert content, with summary alert levels for overall fatality and economic loss estimates, as well as summary content on recent earthquakes, structure vulnerability, and historical secondary hazards. As soon as the Hazus run using the most up-to-date version of the USGS ShakeMap is completed and reviewed by both agencies, the second alert page will be generated and delivered via the USGS website as an update. This second page will contain a summary of the more comprehensive Hazus model results, including spatially distributed estimates of: affected population, economic impact, non-fatal injuries, displaced households, and damage to structures (including potential building safety evaluation requirements). This enhancement does not affect the timeliness and the importance of the initial (onePAGER) alerting content for significant earthquakes; however, the twoPAGER report will provide more detailed, damage and loss/impact content for the Nation in the critical hours following a damaging earthquake.

Conference Paper↗

Individual variation in baseline and stress-induced corticosterone and prolactin levels predicts parental effort by nesting mourning doves

Endocrine systems have an important mechanistic role in structuring life-history trade-offs. During breeding, individual variation in prolactin (PRL) and corticosterone (CORT) levels affects behavioral and physiological processes that drive trade-offs between reproduction and self-maintenance. We examined patterns in baseline (BL) and stress induced (SI; level following a standard capture-restraint protocol) levels of PRL and CORT for breeding mourning doves ( Zenaida macroura ). We determined whether the relationship of adult condition and parental effort to hormone levels in wild birds was consistent with life-history predictions. Both BL PRL and BL CORT level in adults were positively related to nestling weight at early nestling ages, consistent with the prediction of a positive relationship of hormone levels to current parental effort of adults and associated increased energy demand. Results are consistent with the two hormones acting together at baseline levels to limit negative effects of CORT on reproduction while maintaining beneficial effects such as increased foraging for nestling feeding. Our data did not support predictions that SI responses would vary in response to nestling or adult condition. The magnitude of CORT response in the parents to our capture-restraint protocol was negatively correlated with subsequent parental effort. Average nestling weights for adults with the highest SI CORT response were on average 10&ndash;15% lighter than expected for their age in follow-up visits after the stress event. Our results demonstrated a relationship between individual hormone levels and within population variation in parental effort and suggested that hormonal control plays an important role in structuring reproductive decisions for mourning doves.

Hormones and Behavior↗

On the use of high-resolution and deep-learning seismic catalogs for short-term earthquake forecasts: Potential benefits and current limitations

Enhanced earthquake catalogs provide detailed images of evolving seismic sequences. Currently, these data sets take some time to be released but will soon become available in real time. Here, we explore whether and how enhanced seismic catalogs feeding into established short-term earthquake forecasting protocols may result in higher predictive skill. We consider three enhanced catalogs for the 2016–2017 Central Italy sequence, featuring a bulk completeness lower by at least two magnitude units compared to the real-time catalog and an improved hypocentral resolution. We use them to inform a set of physical Coulomb Rate-and-State (CRS) and statistical Epidemic-Type Aftershock Sequence (ETAS) models to forecast the space-time occurrence of M3+ events during the first 6 months of the sequence. We track model performance using standard likelihood-based metrics and compare their skill against the best-performing CRS and ETAS models among those developed with the real-time catalog. We find that while the incorporation of the triggering contributions from new small magnitude detections of the enhanced catalogs is beneficial for both types of forecasts, these models do not significantly outperform their respective near real-time benchmarks. To explore the reasons behind this result, we perform targeted sensitivity tests that show how (a) the typical spatial discretizations of forecast experiments ( 2 km) hamper the ability of models to capture highly localized secondary triggering patterns and (b) differences in earthquake parameters (i.e., magnitude and hypocenters) reported in different catalogs can affect forecast evaluation. These findings will contribute toward improving forecast model design and evaluation strategies for next-generation seismic catalogs.

Journal of Geophysical Research--Solid Earth↗

Effects of capturing and collaring on polar bears: findings from long-term research on the southern Beaufort Sea population

Context: The potential for research methods to affect wildlife is an increasing concern among both scientists and the public. This topic has a particular urgency for polar bears because additional research is needed to monitor and understand population responses to rapid loss of sea ice habitat. Aims: This study used data collected from polar bears sampled in the Alaska portion of the southern Beaufort Sea to investigate the potential for capture to adversely affect behaviour and vital rates. We evaluated the extent to which capture, collaring and handling may influence activity and movement days to weeks post-capture, and body mass, body condition, reproduction and survival over 6 months or more. Methods: We compared post-capture activity and movement rates, and relationships between prior capture history and body mass, body condition and reproductive success. We also summarised data on capture-related mortality. Key results: Individual-based estimates of activity and movement rates reached near-normal levels within 2–3 days and fully normal levels within 5 days post-capture. Models of activity and movement rates among all bears had poor fit, but suggested potential for prolonged, lower-level rate reductions. Repeated captures was not related to negative effects on body condition, reproduction or cub growth or survival. Capture-related mortality was substantially reduced after 1986, when immobilisation drugs were changed, with only 3 mortalities in 2517 captures from 1987–2013. Conclusions: Polar bears in the southern Beaufort Sea exhibited the greatest reductions in activity and movement rates 3.5 days post-capture. These shorter-term, post-capture effects do not appear to have translated into any long-term effects on body condition, reproduction, or cub survival. Additionally, collaring had no effect on polar bear recovery rates, body condition, reproduction or cub survival. Implications: This study provides empirical evidence that current capture-based research methods do not have long-term implications, and are not contributing to observed changes in body condition, reproduction or survival in the southern Beaufort Sea. Continued refinement of capture protocols, such as the use of low-impact dart rifles and reversible drug combinations, might improve polar bear response to capture and abate short-term reductions in activity and movement post-capture.

Wildlife Research↗

Populations, productivity, and feeding habits of seabirds on St. Lawrence Island, Alaska: Final report

A field camp was established at Kongkok Bay near the southwest cape of St. Lawrence Island and occupied continuously from 24 May to 2 September 1987. Permanent study plots were selected for both cliff and crevice-nesting species, and regular observations were made throughout the breeding season to document attendance patterns, breeding phenology, and success. Periodic collections of adults offshore and of chick meals in the colonies were used to determine the food habits of study species. Additional plots for population monitoring of murres and kittiwakes were established in colonies near Savoonga on the north side of the island, and counts were made there between 23 July and 1 August. Shore based work was supplemented with offshore studies of seabird foraging distribution from the USFWS vessel TIGLAX between 18 August and 3 September 1987. Populations of all study species in the Kongkok Bay area increased since the last study of seabirds there (murres 20, kittiwakes 87, Least Auklets 8, Crested Auklets 442). Pelagic Cormorants, Common Murres, and Thick-billed Murres exhibited average, or above average, breeding success at Kongkok in 1987. Black-legged Kittiwakes exhibited near-total reproductive failure. Crested and Least Auklets had high levels of breeding success after the effects of observer disturbance were taken into account. Predation by microtine rodents and foxes was a significant source of chick mortality on auklets. Feeding concentrations were found primarily north of Gambell in the Anadyr Strait or western Chirikof Basin. Kittiwakes were dispersed widely over the study area. Diets of all species studied were normal and, with the exception of kittiwakes, there was no evidence of problems in obtaining food. Current methods for assessing population changes in cliff-nesting species are considered adequate, but better techniques are needed for crevice-nesting auklets. Time-lapse photography offers the greatest potential for monitoring auklet numbers. A protocol is suggested for monitoring populations of murres, kittiwakes, and auklets at colonies in the Bering and Chukchi Seas. It calls for annual visits to selected colonies during two stages of the nesting cycle to assess numbers and breeding productivity. The breeding failure of Black-legged Kittiwakes on St. Lawrence Island in 1987 was part of a pervasive syndrome of failure in this species observed throughout the Bering Sea and Gulf of Alaska in recent years. The causes of recurrent, widespread breeding failure need to be identified if kittiwakes are to have a role in area-wide population monitoring during the period of Alaskan development by the oil and gas industry.

Alaska↗

Population dynamics of the California Spotted Owl (Strix occidentalis occidentalis): a meta-analysis

We conducted a meta-analysis to provide a current assessment of the population characteristics of California Spotted Owls (Strix occidentalis occidentalis) resident on four study areas in the Sierra Nevada and one study area in southern California. Our meta-analysis followed rigorous a priori analysis protocols, which we derived through extensive discussion during a week-long analysis workshop. Because there is great interest in the owl?s population status, we used state-of-the-art analytical methods to obtain results as precise as possible. Our meta-analysis included data from five California study areas located on the Lassen National Forest (1990-2000), Eldorado National Forest (1986-2000), Sierra National Forest (1990-2000), Sequoia and Kings Canyon national parks (1990-2000), and San Bernardino National Forest (1987-1998). Four of the five study areas spanned the length of the Sierra Nevada, whereas the fifth study area encompassed the San Bernardino Mountains in southern California. Study areas ranged in size from 343 km2 (Sequoia and Kings Canyon) to 2,200 km (Lassen). All studies were designed to use capture-recapture methods and analysis. We used survival in a meta-analysis because field methods were very similar among studies. However, we did not use reproduction in a meta-analysis because it was not clear if variation among individual study-area protocols used to assess reproductive output of owls would confound results. Thus, we analyzed fecundity only by individual study area. We examined population trend using the reparameterized Jolly-Seber capture-recapture estimator (8t) We did not estimate juvenile survival rates because of estimation problems and potential bias because of juvenile emigration from study areas. We used mark-recapture estimators under an information theoretic framework to assess apparent survival rates of adult owls. The pooled estimate for adult apparent survival for the five study areas was 0.833, which was lower than pooled adult survival rates (0.850) from 15 Northern Spotted Owl (S. o. caurina) studies. Estimates of survival from the best model on the Lassen (N = 0.829, 95% confidence intervals [CI = 0.798 to 0.857), Eldorado (N = 0.815, 95% CI = 0.772 to 0.851), Sierra (N = 0.818, 95% CI = 0.781 to 0.850), and San Bernardino (N = 0.813, 95% CI = 0.782 to 0.841) were not different. However, the Sequoia and Kings Canyon population had a higher survival rate (N = 0.877, 95% CI = 0.842 to 0.905) than the other study areas. Management history and forest structure (e.g. presence of giant sequoia [Sequoiadendron giganteum]) on the Sequoia and Kings Canyon study area differed from all other study areas. There appears to be little or no evidence for temporal variation in adult apparent survival on any of the study areas. Although we did not directly compare fecundity estimates were highly variable among years within all study areas (CV of temporal process variation = 0.672-0.817). Estimates for fecundity among the study populations were Lassen (b = 0.336, SE = 0.083), Eldorado (b = 0.409, SE = 0.087), Sierra (b = 0.284, SE = 0.073), Sequoia and Kings Canyon (b = 0.289, SE = 0.074), and San Bernardino (b = 0.362, SE = 0.038). During most years, the Sierra Nevada populations showed either moderate or poor fecundity. However, 1992 appeared to be an exceptional reproductive year for owls in the Sierra Nevada. In contrast, the San Bernardino population had less variable reproduction (CV of temporal process variation = 0.217), but experienced neither the exceptional reproduction of 1992 nor the extremely poor years that characterized all of the Sierra Nevada study areas. Because fecundity may be influenced by weather patterns, it was possible that the different weather patterns between southern California and the Sierra Nevada accounted for that difference. Except for Eldorado, all estimates for 8t, were <1.0, but none was different from 8 = 1.0 given the 95% confidence i

Ornithological Monographs↗

Biodegradability of dissolved organic carbon in permafrost soils and aquatic systems: a meta-analysis

As Arctic regions warm and frozen soils thaw, the large organic carbon pool stored in permafrost becomes increasingly vulnerable to decomposition or transport. The transfer of newly mobilized carbon to the atmosphere and its potential influence upon climate change will largely depend on the degradability of carbon delivered to aquatic ecosystems. Dissolved organic carbon (DOC) is a key regulator of aquatic metabolism, yet knowledge of the mechanistic controls on DOC biodegradability is currently poor due to a scarcity of long-term data sets, limited spatial coverage of available data, and methodological diversity. Here, we performed parallel biodegradable DOC (BDOC) experiments at six Arctic sites (16 experiments) using a standardized incubation protocol to examine the effect of methodological differences commonly used in the literature. We also synthesized results from 14 aquatic and soil leachate BDOC studies from across the circum-arctic permafrost region to examine pan-arctic trends in BDOC. An increasing extent of permafrost across the landscape resulted in higher DOC losses in both soil and aquatic systems. We hypothesize that the unique composition of (yedoma) permafrost-derived DOC combined with limited prior microbial processing due to low soil temperature and relatively short flow path lengths and transport times, contributed to a higher overall terrestrial and freshwater DOC loss. Additionally, we found that the fraction of BDOC decreased moving down the fluvial network in continuous permafrost regions, i.e. from streams to large rivers, suggesting that highly biodegradable DOC is lost in headwater streams. We also observed a seasonal (January&ndash;December) decrease in BDOC in large streams and rivers, but saw no apparent change in smaller streams or soil leachates. We attribute this seasonal change to a combination of factors including shifts in carbon source, changing DOC residence time related to increasing thaw-depth, increasing water temperatures later in the summer, as well as decreasing hydrologic connectivity between soils and surface water as the thaw season progresses. Our results suggest that future climate warming-induced shifts of continuous permafrost into discontinuous permafrost regions could affect the degradation potential of thaw-released DOC, the amount of BDOC, as well as its variability throughout the Arctic summer. We lastly recommend a standardized BDOC protocol to facilitate the comparison of future work and improve our knowledge of processing and transport of DOC in a changing Arctic.

Biogeosciences↗

A pilot biodiversity inventory and monitoring protocol in support of coastal adaptation projects in tidal and nearshore subtidal habitats of Boston Harbor Islands

The Boston Harbor Islands National Recreation Area (BOHA) is at high risk to the impacts of sealevel rise (SLR) and erosion from coastal storms. In June 2021, the National Trust for Historic Preservation listed the islands as one of America’s 11 Most Endangered Historic Places due to climate change. BOHA partners have been working to find climate adaptive solutions to protect and sustain critical ecological and cultural resources on the islands. A range of coastal adaptation efforts are currently under consideration including increased shoreline armoring and nature-based adaptation solutions. Any action taken in the coastal zone will require an assessment of environmental and ecological communities that could potentially be impacted by disturbance caused by restoration or adaptation projects. The primary goals of the initial phase of this project were to: 1) synthesize occurrence and distribution records of biodiversity living in and using mixed coarse substrate habitats of the intertidal zone of the Boston Harbor Islands; and 2) identify and compile potential methods to develop a standard and repeatable monitoring protocol to track changes (natural or anthropogenic) in intertidal biodiversity over time and across locations; and 3) conduct preliminary site scoping of target islands to identify locations for collecting new baseline data. A biodiversity inventory list was compiled, showing a total of 451 unique species were observed in BOHA between 1861-2020. Of this list, 55 species (invertebrates: 47; algae: 8) were considered nonindigenous species; a watchlist was also developed to help BOHA partners identify potential future invaders that could colonize and impact intertidal communities due to ongoing climate change or disturbance events. Native species observed in BOHA were evaluated using existing conservation frameworks and climate vulnerability information to prioritize species at greatest risk from anthropogenic and environmental stressors for future actions. Lastly, site scoping activities during 2021 identified three types of sites for future intertidal monitoring initiatives: (1) sites with relatively high biodiversity and foundational species, (2) erosional sites near cultural areas of importance to NPS, and (3) sites with generic (common across islands) biodiversity. Overall results are anticipated to help the NPS and BOHA partners identify a suite of species and sites for future monitoring given anticipated adaptation projects and ongoing changes due to SLR, coastal storms and other stressors.

Massachusetts↗

Study design and methods for a wetland condition assessment on U.S. Fish and Wildlife Service fee-title lands in the Prairie Pothole Region of North Dakota, South Dakota, and Montana, USA

The U.S. Fish and Wildlife Service (FWS) manages wetlands and grasslands for wildlife habitat throughout the central North American Prairie Pothole Region (PPR). PPR wetlands, or potholes, are widely recognized as critical habitats for North American migratory waterfowl, waterbirds, and other wildlife. Potholes also provide other ecosystem services such as carbon sequestration, flood mitigation, filtration of pollutants, groundwater recharge, nutrient retention, and recreational opportunities. Wetland condition assessments have been completed nationally at coarse scales, but focused, regionwide assessments of the biological condition of potholes managed by the FWS are lacking. Therefore, FWS personnel require information pertaining to the biological condition and status of wetlands on FWS fee-title lands in the PPR to support management, restoration, and acquisition efforts. The biological condition of wetlands typically is reflected by their plant communities, and these communities correspond to past and current management and anthropogenic disturbances; thus, plant communities are a suitable surrogate of wetland condition. This report describes the study design, selection of sample sites, and field survey methods for a wetland condition assessment for FWS fee-title lands in the PPR of North Dakota, South Dakota, and Montana. Various spatial databases were gathered (for example, National Wetlands Inventory) to identify and assess potholes on FWS fee-title lands and to facilitate the selection of study sites. A spatially balanced, site-selection process resulted in the inclusion of 125 temporarily and 125 seasonally ponded potholes distributed across the area of interest; the first 100 for each classification were considered the primary study sites, whereas the remaining 25 were considered an oversample to replace those deemed not appropriate for sampling by field crews. Study sites were within native prairie and reseeded grasslands on FWS National Wildlife Refuges and Waterfowl Production Areas and are distributed among the primary physiographic subregions of the PPR: the Glaciated Plains, Missouri Coteau, and Prairie Coteau; a small number of sites also are within the Lake Agassiz Plain and Turtle Mountains. Site assessment protocols, vegetation survey methods, data analyses, and condition categories (for example, poor, good, very good) for the wetland assessment are based on the North Dakota Rapid Assessment Method and an Index of Plant Community Integrity developed for potholes. Results of the wetland condition assessment will aid FWS staff in assessing past and current management and help to identify priority areas for future management and acquisition.

North Dakota, South Dakota, Montana↗

Research plan for lands administered by the U.S. Department of the Interior in the Interior Columbia Basin and Snake River Plateau

This document presents a long-term research strategy designed to address current and future research needs for management of Department of the Interior-administered ecosystems in the Intermountain West. Although the research plan was developed in the context of the Interior Columbia Basin Ecosystem Management Project, the plan addresses many high-priority issues facing land managers throughout the Intermountain West. These issues pose management challenges that may be addressed with applied research both currently and in upcoming decades. Possessing a particular focus on semiarid ecosystems, the plan is a collection of research questions under five categories of research emphases: 1) restoration; 2) rangeland health; 3) aquatic-terrestrial connections; 4) development of monitoring and evaluation protocols; and 5) species and habitats at risk. The goal of the research strategy is to provide ideas for integrating emerging scientific understanding into future management in order to restore and maintain long-term ecosystem health and ecological integrity; provide consistent management direction over broad spatial and temporal scales; emphasize adaptive management over the long term; restore and maintain habitats for plant and animal species; and support economic and social needs of people, without compromising the above goals. Research questions are prioritized into three categories based on the immediacy of their need, feasibility of addressing the question rigorously under varying funding budgets, and magnitude of risk posed by not addressing the issue. The research strategy is intended to support and integrate with existing management efforts and strategies. As such, it melds observational studies with experimental manipulation, treating management actions as experiments whenever feasible. The research strategy focuses on disturbance processes and events that have been the primary drivers of change, to provide a predictive model for future changes. These drivers include fire, nonnative plants, herbivory, roads and associated human influences, and climate change. Whereas management in the western United States has striven to move from an inefficient species-based approach to a habitat-based approach, the plan focuses on ecosystem function and ecological processes as critical measures of habitat response. Because of the large amount and contiguity of public lands in the western United States, the region presents both a compelling opportunity to implement landscape-level science and a challenge to underst

Information and Technology Report↗

The use of forward looking infrared to locate bird carcasses in agricultural areas

Helicopter-mounted Forward Looking Infrared has mainly been used for large animal censuses. I examined the use of this instrument in locating bird carcasses in agricultural fields to improve current carcass searching techniques. Mallard (Arias platyrhynchos) and northern bobwhite quail (Colinus virginianus) carcasses were measured with an infrared thermometer immediately following death and for 5 consecutive nights to determine the optimal time for detection. Preliminary flights were conducted to design a protocol that was used in test flights. Bird species (mallard versus quail) and cover type (bare ground versus short grass) were compared in the flights. Carcasses were recovered with the aid of Global Positioning Systems. Carcasses remained above ambient ground temperatures for all or part of night 1. Quail carcass temperatures decreased faster than mallard carcasses. In warmer weather, carcass temperatures increased 3-5 nights following death. In colder weather, carcasses were 1-2 C cooler than the ground after the first night. Mallard and quail carcasses were both detected on bare ground and short grass cover types with Forward Looking Infrared. The carcass recovery rates were 40% arid 30% on bare ground and short grass, respectively. There were no significant differences in detection for species or cover type. In warmer weather, carcasses could be detected for several hours following death and again 3-5 nights after death. Carcasses may be detected as objects cooler than the ground in colder weather. Forward Looking Infrared was successful in detecting mallard and quail carcasses. Further research should evaluate improved mapping techniques to enhance carcass recovery.

Book↗

Effect of analytical conditions in wavelength dispersive electron microprobe analysis on the measurement of strontium-to-calcium (Sr/Ca) ratios in otoliths of anadromous salmonids

The use of strontium-to-calcium (Sr/Ca) ratios in otoliths is becoming a standard method to describe life history type and the chronology of migrations between freshwater and seawater habitats in teleosts (e.g. Kalish, 1990; Radtke et al., 1990; Secor, 1992; Rieman et al., 1994; Radtke, 1995; Limburg, 1995; Tzeng et al. 1997; Volk et al., 2000; Zimmerman, 2000; Zimmerman and Reeves, 2000, 2002). This method provides critical information concerning the relationship and ecology of species exhibiting phenotypic variation in migratory behavior (Kalish, 1990; Secor, 1999). Methods and procedures, however, vary among laboratories because a standard method or protocol for measurement of Sr in otoliths does not exist. In this note, we examine the variations in analytical conditions in an effort to increase precision of Sr/Ca measurements. From these findings we argue that precision can be maximized with higher beam current (although there is specimen damage) than previously recommended by Gunn et al. (1992).

Fishery Bulletin↗

Brook Floater rapid assessment monitoring protocol

The Brook Floater ( Alasmidonta varicosa ) is a small (<100 mm), stream dwelling freshwater mussel (Family: Unionidae) from Atlantic Slope drainages in the eastern U.S. (Nedeau 2008). Brook Floater have dramatically contracted in distribution over recent decades, and there is limited evidence of recruitment in most locations, despite minimal effort to document population status (Wicklow et al., 2017). Brook Floater is listed as a Species of Greatest Conservation Need (SGCN) throughout its range in the United States (state-listed as imperiled or critically imperiled in all 15 states), has been extirpated from two states (Rhode Island and Delaware) and was recently petitioned for Federal listing in 2011 (Wicklow et al. 2017). Currently, there is a U.S. Fish and Wildlife (USFWS) Species Status Assessment underway to determine if federal listing under the Endangered Species Act is warranted. Brook Floater is also listed as a species of special concern in Canada, the northern extent of its range. In 2016, a state wildlife grant was awarded to develop range-wide conservation initiatives and strategies, including the development of rapid assessment and long-term monitoring techniques, in addition to developing conservation strategies to improve its probability of persistence in the future. The purpose of this protocol is to describe and facilitate a rapid approach to estimating Brook Floater occupancy to better understand the factors that influence Brook Floater distribution. Occupancy estimation approaches allow for estimation of species occupancy (; percent area occupied) within some scale of interest (for our purposes, the watershed), while simultaneously estimating species detection probability (p; the probability of finding an organism, if present). Occupancy estimation has been used with many wildlife taxa and is essential for understanding the presence or absence of wildlife in a particular area while accounting for imperfect detection (i.e., p<1; MacKenzie et al. 2004, Shea et al. 2013, Wisniewski et al. 2013, Pandolfo et al. 2016, MacKenzie 2016). This approach does not rely on existing information about species presence or absence to select sites. Occupancy estimation operates on a robust probabilistic framework of randomly selected sites to infer what proportion of sites are occupied. Occupancy estimation also incorporates imperfect detection (p <1; i.e., animals are cryptic and elusive; observers have varying experience searching, etc.; MacKenzie et al. 2003). For example, two mussel species that occupy a site might have two very different detection histories, as determined by revisiting a site and using the same methods on repeated visits to find both species. See hypothetical results here: Visits 1 2 3 4 5 Mussel species A 1 1 1 0 1 Mussel species B 0 0 0 1 0 (1 = detected, 0=not detected) Both of these mussel species occupy this site, yet Mussel A was detected in 4 out of 5 visits (high p) and Mussel B was detected in 1 out of 5 visits (low p) with the methods used to survey this site. Covariates may explain differences in detection between species or visits. Organisms may be: 1) present and not observed, 2) present and unavailable for capture (i.e., buried in sediment), or 3) not present at the site. Occupancy estimation uses repeated visits of randomly selected sites to build species detection histories (i.e., 1, 0, 1) to simultaneously estimate occupancy () and p. Typically, repeated visits are discrete sampling events and are more time consuming because each site requires >3 separate visits. In our rapid assessment protocol, we use multiple independent observers searching longitudinal lanes to estimate detection in a single site visit as opposed to multiple discrete visits. Below are hypothetical results of occupancy by observer: Independent Observers 1 2 3 4 5 Mussel species A 1 1 0 1 1 Mussel species B 1 0 0 1 0 (1 = detected, 0=not detected) Objectives: The objectives of this rapid assessment survey approach are to guide collection of data that can be used to: A. Estimate the occupancy of Brook Floater within watersheds. B. Estimate the effects of reach- and watershed-scale habitat features on Brook Floater occurrence. C. Understand how survey covariates (e.g., surveyor experience) influence detection of Brook Floater. While this protocol explicitly targets collection of Brook Floater, it is likely that the methods can be adapted for occupancy surveys of other stream-dwelling freshwater mussel species.

Cooperator Science Series↗

Resources to inform the 2026 Colorado Climate Preparedness Roadmap: Compound hazards and associated public health implications

Changing environmental conditions are increasing the frequency and severity of extreme weather and environmental hazards, including heat waves, wildfires, drought, reduced water quality, and degraded air quality from ozone (O3) and fine particulate matter pollution (AghaKouchak et al., 2020; Bolinger et al., 2024; Colorado Health Institute, 2023a; Fann et al., 2015; IPCC, 2023a; Nagamoto et al., 2024; Nolte et al., 2018, 2021; Zscheischler et al., 2020). Strong scientific evidence indicates that climate change will increase risks to public health and safety throughout the 21st century, particularly through compound climate events (AghaKouchak et al., 2020; Cascio, 2018; Childs et al., 2022; Ebi, 2025; IPCC, 2023a, 2023b; Ma et al., 2024; Marlier et al., 2026; National Academy of Medicine, 2025; Reid et al., 2016; Reid & Maestas, 2019; Simpson et al., 2023; van den Hurk et al., 2023; Volckens, 2024; Zscheischler et al., 2018, 2020). Compound hazards, which are co-occurring or sequential hazards such as heat waves occurring alongside drought, wildfire, and poor air quality from smoke, can amplify public health risks beyond the effects of individual hazards alone (Marlier et al., 2026; Zscheischler et al., 2020). In Colorado, compound climate events with clear implications for public health are already evident (see “Core definitions” section for additional context on compound climate events). Recent statewide assessments have documented increasing heat waves, drought, and wildfires linked to rising temperatures, while emerging data show that climate change is reversing decades of air quality improvements through increased O3 and fine particulate matter pollution due to wildfire, and increased aerosolized topsoil (i.e., dust) due to drought (Bolinger et al., 2024; Childs et al., 2022; Fann et al., 2015; Ghanbari et al., 2023; Larsen et al., 2022; Nolte et al., 2018, 2021; Volckens, 2024). These environmental shifts are associated with increases in eye and kidney-health related concerns, heat-related illness, exacerbations of asthma and other respiratory conditions, increased risk of infectious disease, cardiovascular stress, and mental health impacts, with disparities in risk for children, older adults, outdoor workers, and communities with existing health and economic vulnerabilities (Baraghoshi et al., 2023; Butler-Dawson et al., 2026; Cascio, 2018; Cleland et al., 2022; Colorado Health Institute, 2023a; Grover et al., 2024; Lee et al., 2023; Ma et al., 2024; Macaluso et al., 2024; Marlier et al., 2026; National Academy of Medicine, 2025; Patnaik et al., 2024; Peters et al., 2019; Reid et al., 2016; Reid & Maestas, 2019). The state is actively pursuing strategies to mitigate the risks of these compound climate events on the public, including expanding climate and health surveillance, strengthening early-warning systems and public communication around smoke and air quality, and developing integrated policy agendas that link climate mitigation, adaptation, and health equity (CDPHE, 2025; Colorado Health Institute, 2023b; Zemaitis & Bailey, 2024). For example, state and partner organizations have articulated cross-sector goals to prepare for health impacts related to extreme heat, wildfires, flooding, drought, declines in water quality, and poor air quality, including updating workplace safety protocols, enhancing community-based resilience, adapting clinical care assessments to include climate-related stressors, and improving access to mental and behavioral health services following climate-related disasters (Colorado Health Institute, 2023b; CDPHE, 2025; Zemaitis & Bailey, 2024). To better prepare for current risks and make informed decisions, more information is needed, including methods to analyze historical and projected conditions in near real time, characterize interactions among compound hazards, and translate climate and air quality science into actionable guidance for public health agencies, health care systems, and communities across Colorado (Ebi, 2025; Marlier et al., 2026; National Academy of Medicine, 2025; Simpson et al., 2023; Zscheischler et al., 2018). For this report, we focus on extreme heat and outdoor air quality (ozone [O3] and fine particulate matter [PM2.5]) as the main compound hazards causing compound climate events, given that 1) the state of Colorado has experienced poor air quality for some time, 2) these climate events are affecting large population numbers, and 3) there is capacity to consider and respond to these climate-driven events.

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