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Handbook of acute toxicity of chemicals to fish and aquatic invertebrates : summaries of toxicity tests conducted at Columbia National Fisheries Research Laboratory, 1965-78

Acute toxicity is a major subject of research at Columbia National Fisheries Research Laboratory for evaluating the impact of toxic chemicals on fishery resources. The Laboratory has played a leading role in developing research technology for toxicity testing and data interpretation. In 1965-78, more than 400 chemicals were tested against a variety of invertebrates and fish species representative of both cold- and warm-water climates. The use of acute toxicity tests for assessing the potential hazard of chemical contaminants to aquatic organisms is well documented (Boyd 1957; Henderson et al. 1960; Sanders and Cope 1966; Macek and McAllister 1970). Static acute toxicity tests provide rapid and (within limits) reproducible concentration-response curves for estimating toxic effects of chemicals on aquatic organisms. These tests provide a database for determining relative toxicity of a large number of chemicals to a variety of species and for estimating acute effects of chemical spills on natural aquatic systems; they also assist in determining priority and design of additional toxicity studies. Acute toxicity tests usually provide estimates of the exposure concentration causing 50% mortality (LC50) to test organisms during a specified period of time. For certain invertebrates, the effective concentration is based on immobilization, or some other identifiable endpoint, rather than on lethality. The application of the LC50 has gained acceptance among toxicologists and is generally the most highly rated test for assessing potential adverse effects of chemical contaminants to aquatic life (Brungs and Mount 1978; American Institute for Biological Sciences 1978 a ). The literature contains numerous papers dealing with the acute toxicity of chemicals to freshwater organisms. However, there is a tremendous need for a concise compendium of toxicity data covering a large variety of chemicals and test species. This Handbook is a compilation of a large volume of acute toxicity data from the Columbia Laboratory and its field laboratories. It presents definitive acute toxicity data on 271 chemicals tested against a variety of freshwater invertebrates and fishes. The chemicals represent all major groups of pesticides, as well as numerous industrial chemicals. This compilation should serve as a useful database for the many agencies and organizations dealing with research and management programs concerned with the impact of chemicals on aquatic resources. The Columbia Laboratory has played a major role in developing currently used standard methodology for static acute toxicity testing. The use of standardized methodology greatly reduces variation in results. The data presented here have been carefully scrutinized to eliminate tests that failed to follow acceptable procedures. Handling of test organisms and procedures for static toxicity tests followed those described by Lennon and Walker (1964) and Macek and McAllister (1970), and conform well with those recommended by Brauhn and Schoettger (1975) and the Committee on Methods for Toxicity Tests with Aquatic Organisms (1975). The species of fish and invertebrates that were tested are listed in phylogenetic order in Tables 1 and 2. Fish were obtained from Federal and State hatcheries as either eggs or fry. Original stocks of invertebrates were collected and cultured from wild populations with no known source of contamination; these populations were replenished regularly. The invertebrates were cultured in the Laboratory by methods similar to those described by Sanders and Cope (1966). Test chemicals usually consisted of technical or analytical grade samples of known purity. Formulations of the chemicals were also tested when available. When purity of test chemicals was known, all calculated concentrations were based on percent active ingredients. Stock solutions were prepared immediately before each test, with commercial grade acetone as the carrier solvent. Occasionally, ethanol or dimethyl-formamide was substituted. Solvent concentrations did not exceed 0.5 mL/L in final dilution water. Test water (dilution water) was reconstituted from deionized water of at least 10 6 ohms resistivity by the addition of appropriate reagent grade chemicals (Marking 1969). Water was buffered to maintain a pH of 7.2 to 7.5, an alkalinity of 30 to 35 mg/L, and a hardness of 40 to 50 mg/L as CaCO 3 . Test water was mixed thoroughly and aerated before transfer into test chambers. Fish were acclimated to dilution water by gradually changing the water in acclimated tanks from 100% well water to 100% reconstituted water over a 1- to 3-day period at the desired testing temperature. Invertebrates were acclimated from well water to dilution water over a 4- to 6-h period. Toxicity tests were conducted under static conditions without aeration, and the organisms were not fed during acclimation or testing. Temperature of test solutions was maintained within ± 1°C of that required for a given test. Toxicity tests with fish were conducted in 18.9-liter (5-gal) wide-mouthed jars containing 15 liters of test solution. Fingerling fish weighing 0.2 to 1.5 g were tested at each concentration. Caution was taken not to exceed 0.8 g of test organisms per liter of solution. Duplicate test chambers were used to accommodate larger fish. Test chambers varied in size for invertebrates, depending on the species used; volume of test solution ranged from 0.25 to 4 liters. At least 10 organisms were exposed to each concentration for all definitive tests. At least six concentrations were used per toxicity test. The tests began upon initial exposure to the toxicant and continued for 96 h. Immobilization tests with invertebrates were conducted for only 48 h. The number of dead or affected organisms in each test chamber were recorded and the dead organisms were removed every 24 h; general observations on the condition of test organisms were also recorded at these times. Toxicity data were analyzed by a statistical method described by Litchfield and Wilcoxon (1949) to determine LC50 (theoretical estimate of the concentration lethal to 50% of the test animals) and 95% confidence intervals. This method is recommended by the American Public Health Association (1971) and by Sprague (1969) for determining median lethal concentrations. The procedure is easily modified for computing a single LC50 when replicate tests are performed.

Resource Publication↗

A rapid field test for sylvatic plague exposure in wild animals

Plague surveillance is routinely conducted to predict future epizootics in wildlife and exposure risk for humans. The most common surveillance method for sylvatic plague is detection of antibodies to Yersinia pestis F1 capsular antigen in sentinel animals, such as coyotes ( Canis latrans ). Current serologic tests for Y. pestis , hemagglutination (HA) test and enzyme-linked immunosorbent assay (ELISA), are expensive and labor intensive. To address this need, we developed a complete lateral flow device for the detection of specific antibodies to Y. pestis F1 and V antigens. Our test detected anti-F1 and anti-V antibodies in serum and Nobuto filter paper samples from coyotes, and in serum samples from prairie dogs ( Cynomys ludovicianus ), lynx ( Lynx canadensis ), and black-footed ferrets ( Mustela nigripes ). Comparison of cassette results for anti-F1 and anti-V antibodies with results of ELISA or HA tests showed correlations ranging from 0.68 to 0.98. This device provides an affordable, user-friendly tool that may be useful in plague surveillance programs and as a research tool.

Colorado, Montana, New Mexico↗

Olympic Fisher Reintroduction Project: Progress report 2008-2011

This progress report summarizes the final year of activities of Phase I of the Olympic fisher restoration project. The intent of the Olympic fisher reintroduction project is to reestablish a self-sustaining population of fishers on the Olympic Peninsula. To achieve this goal, the Olympic fisher reintroduction project released 90 fishers within Olympic National Park from 2008 to 2010. The reintroduction of fishers to the Olympic Peninsula was designed as an adaptive management project, including the monitoring of released fishers as a means to (1) evaluate reintroduction success, (2) investigate key biological and ecological traits of fishers, and (3) inform future reintroduction, monitoring, and research efforts. This report summarizes reintroduction activities and preliminary research and monitoring results completed through December 2011. The report is non-interpretational in nature. Although we report the status of movement, survival, and home range components of the research, we have not completed final analyses and interpretation of research results. Much of the data collected during the monitoring and research project will be analyzed and interpreted in the doctoral dissertation being developed by Jeff Lewis; the completion of this dissertation is anticipated prior to April 2013. We anticipate that this work, and analyses of other data collected during the project, will result in several peer-reviewed scientific publications in ecological and conservation journals, which collectively will comprise the final reporting of work summarized here. These publications will include papers addressing post-release movements, survival, resource selection, food habits, and age determination of fishers.

Report↗

Granular avalanches across irregular three-dimensional terrain: 1. Theory and computation

To establish a theoretical basis for predicting and interpreting the behavior of rapid mass movements on Earth's surface, we develop and test a new computational model for gravity-driven motion of granular avalanches across irregular, three-dimensional (3-D) terrain. The principles embodied in the model are simple and few: continuum mass and momentum conservation and intergranular stress generation governed by Coulomb friction. However, significant challenges result from the necessity of satisfying these principles when deforming avalanches interact with steep and highly variable 3-D terrain. We address these challenges in four ways. (1) We formulate depth-averaged governing equations that are referenced to a rectangular Cartesian coordinate system (with z vertical) and that account explicitly for the effect of nonzero vertical accelerations on depth-averaged mass and momentum fluxes and stress states. (2) We compute fluxes of mass and momentum across vertical cell boundaries using a high-resolution finite volume method and Roe-type Riemann solver. Our algorithm incorporates flux difference splitting, an entropy correction for the flux, and eigenvector decomposition to embed the effects of driving and resisting forces in Riemann solutions. (3) We use a finite element method and avalanche displacements predicted by Riemann solutions to compute Coulomb stresses conjugate to the displacements in 3-D stress space. (4) We test the model output against analytical solutions, a sand cone conceptual experiment, and (in a companion paper) data from detailed laboratory experiments. Model results illustrate a complex interplay of basal traction and internal stress, and they successfully predict not only the gross behavior but also many details of avalanche motion from initiation to deposition.

Journal of Geophysical Research Earth Surface↗

Hydrodynamic and salinity tesponse to tidal restoration in the Herring River Estuary, MA, considering present and future sea levels

Coastal salt marshes are crucial ecosystems that provide habitat for a variety of species, improve water quality, and play a major role in the global carbon cycle. However, many salt marshes have been severely damaged by human activities such as diking and draining for urban development. Recently, there has been a noticeable shift toward the prioritization of coastal marsh restoration to re-establish their ecosystem services. The removal of anthropogenic barriers such as dikes, sluices, and culverts is a critical component of many projects because it allows for the restoration of tidal flow to support natural hydrologic regimes and salinity conditions, which play a dominant role in determining the ecological and biogeochemical functioning of marshes. This study examines how proposed removal of hydraulic structures will influence the hydrologic potential for marsh restoration in the Herring River Estuary in Cape Cod, Massachusetts, USA. Construction of dikes, roadways, and low-capacity culverts over the last century has substantially restricted tidal flow in the Herring River Estuary, leading to degradation of salt marsh habitat. The estuary is now undergoing the first phase of a restoration project to re-introduce natural hydrologic conditions, increase salinity, and restore salt marsh habitat. To assess how the Herring River Estuary will respond to human- and climate-driven modifications, we develop and apply a validated hydrodynamic model to simulate the complex tidal and salinity dynamics of the estuary under a range of restoration and sea level rise scenarios. We then quantify how salinity and critical hydrologic variables, including tidal range and depth of mean high water, will evolve for various restoration scenarios considering present and future sea levels. The results of this research can inform coastal management and restoration plans that re-create the natural functioning of the system while protecting critical infrastructure and reducing the risk of restoration failure.

Massachusetts↗

Research on polar bears in Alaska, 1983-1985

Research on the ecology and status of polar bear populations in Alaska has continued since 1967. Research was a joint U.S. Fish and Wildlife Service/Alaska Department of Fish and Game effort until passage of the Marine Mammal Protection Act in 1972, and has been largely a Federal effort since then. In 1985, Alaskan polar beer research continues to be carried out by the Research Division of the U.S. Fish and Wildlife Service (DOI). A recent reorganization removed authority for ecological research in Alaska from the Denver Wildlife Research Center, and vested it with the newly created Alaska Office of Fish and Wildlife Research. This new research office is the center for Federal fish and Wildlife related research throughout the state of Alaska and in its coastal waters. Although the responsibility for polar bear research lies with the U.S. Fish and Wildlife Service, numerous other organizations and agencies deserve mention for their cooperation and support of the ongoing research. These include: the U.S. National Oceanic and Atmospheric Administration (DOC), The U.S. Minerals Management Service (DOI), The Canadian Wildlife Service, The Northwest Territories Wildlife Service, the Yukon Wildlife Service, Dome Petroleum Ltd, Gulf Canada, and the Alaska Department of Fish and game.

Alaska↗

Development of small uncrewed aerial systems for multi-instrument geophysical data acquisition in active geothermal systems

Small Uncrewed Aerial Systems (sUAS) serve as critical platforms for geophysical data collection at an intermediate scale between lower resolution, regional datasets collected via crewed aerial surveys, and high resolution, but spatially sparse sampling of ground-based data collection methods. Advances in sensor design and sUAS capabilities have led to rapid advances in the amount and type of geophysical data that can be acquired using sUAS-based survey designs (Gavazzi et al., 2019). Here we showcase the utility of a single sUAS (the Matrice 600 Pro and accompanying sensor package) that collects magnetic, thermal infra-red (TIR) and gas (CO2, SO2, H2S, water vapor) data for use in geothermal resource exploration and monitoring, with case studies in eastern California and Iceland. The work highlights the flexibility of modern sUAS systems for single-team acquisition of multiple independent but coupled geophysical data which allow for a multidisciplinary approach to geothermal systems research. We summarize the workflows involved in collecting each dataset as well as several common issues encountered both during data collection and data processing.

Conference Paper↗

Integrated environmental modeling: a vision and roadmap for the future

Integrated environmental modeling (IEM) is inspired by modern environmental problems, decisions, and policies and enabled by transdisciplinary science and computer capabilities that allow the environment to be considered in a holistic way. The problems are characterized by the extent of the environmental system involved, dynamic and interdependent nature of stressors and their impacts, diversity of stakeholders, and integration of social, economic, and environmental considerations. IEM provides a science-based structure to develop and organize relevant knowledge and information and apply it to explain, explore, and predict the behavior of environmental systems in response to human and natural sources of stress. During the past several years a number of workshops were held that brought IEM practitioners together to share experiences and discuss future needs and directions. In this paper we organize and present the results of these discussions. IEM is presented as a landscape containing four interdependent elements: applications, science, technology, and community. The elements are described from the perspective of their role in the landscape, current practices, and challenges that must be addressed. Workshop participants envision a global scale IEM community that leverages modern technologies to streamline the movement of science-based knowledge from its sources in research, through its organization into databases and models, to its integration and application for problem solving purposes. Achieving this vision will require that the global community of IEM stakeholders transcend social, and organizational boundaries and pursue greater levels of collaboration. Among the highest priorities for community action are the development of standards for publishing IEM data and models in forms suitable for automated discovery, access, and integration; education of the next generation of environmental stakeholders, with a focus on transdisciplinary research, development, and decision making; and providing a web-based platform for community interactions (e.g., continuous virtual workshops).

Environmental Modelling and Software↗

China's rare-earth industry

Introduction China's dominant position as the producer of over 95 percent of the world output of rare-earth minerals and rapid increases in the consumption of rare earths owing to the emergence of new clean-energy and defense-related technologies, combined with China's decisions to restrict exports of rare earths, have resulted in heightened concerns about the future availability of rare earths. As a result, industrial countries such as Japan, the United States, and countries of the European Union face tighter supplies and higher prices for rare earths. This paper briefly reviews China's rare-earth production, consumption, and reserves and the important policies and regulations regarding the production and trade of rare earths, including recently announced export quotas. The 15 lanthanide elements-lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, and lutetium (atomic numbers 57-71)-were originally known as the rare earths from their occurrence in oxides mixtures. Recently, some researchers have included two other elements-scandium and yttrium-in their discussion of rare earths. Yttrium (atomic number 39), which lies above lanthanum in transition group III of the periodic table and has a similar 3+ ion with a noble gas core, has both atomic and ionic radii similar in size to those of terbium and dysprosium and is generally found in nature with lanthanides. Scandium (atomic number 21) has a smaller ionic radius than yttrium and the lanthanides, and its chemical behavior is intermediate between that of aluminum and the lanthanides. It is found in nature with the lanthanides and yttrium. Rare earths are used widely in high-technology and clean-energy products because they impart special properties of magnetism, luminescence, and strength. Rare earths are also used in weapon systems to obtain the same properties.

Open-File Report↗

This week?s Citation Classic: 'Stickel, L. F. A comparison of certain methods of measuring ranges of small mammals. J. Mammalogy 35: 1-15, 1954.'

During my first employment as a junior biologist conducting food habits analyses at the Patuxent Research Refuge (now Patuxent Wildlife Research Center) of the US Fish and Wildlife Service, the first station director, Arnold L. Nelson, gave each staff biologist the opportunity to spend a little time conducting field studies on the 2,600-acre research area. These studies were to help in ecological evaluation of the area, but also, no doubt, functioned as a morale builder for biologists daily engaged in difficult, demanding and confining laboratory work. I undertook to measure the population density of small mammals in different habitats. The state-of-the-art methods recommended to me and prevalent in the literature worried me, especially after preliminary field work. Reading the classic, paper by W.H. Burt [Terrioriality and home range concepts as applied to mammals. J. Mammalogy 24:346-352, 1943] was a turning point for me in seeing that knowledge of home-range size was the key to measuring population density. My first papers employed these concepts in field evaluations. However, they could give-only limited attention to the question of how home ranges could be measured. Furthermore, the importance of knowledge of the home range to habitat evaluation and to understanding animal behavior was becoming increasingly apparent. Advocacy of many different methods of measuring home ranges appeared in the literature. It did not seem possible to evaluate them objectively. It occurred to me that artificial populations could be used to help in understanding the mechanisms of measuring home ranges. The effects of random trap-visiting, bias toward central traps, trap-spacing, range shape, and other factors could be considered. So grids of traps were inked onto oversize graph paper, and simulated ranges were cut from plastic to be tossed as randomly as possible on the trap-grids. It was a slow, laborious job. Analysis showed that random captures in artificial populations produced patterns that were similar in many ways to those observed in field studies; the necessity of appropriate trap-spacing in relation to range size became obvious. Some commonly used methods of expressing range size proved to be clearly superior to others. However, the artificial populations could not fully simulate actual populations, where use patterns changed as conditions changed and the ?boundaries? of ranges tended to shift continuously or with time. Progress was made, but problems remained. I suspect that the reasons the paper has been cited frequently rest on the continuing interest in home-range behavior and the continuing difficulty in field evaluations, despite the many advances in observational methodology. The sophisticated techniques that became possible with the use of computers [R.I. Jennrich and F.B. Turner. Measurement of non-circular home range. J. Theor. Biol 23:227-237, 1969] also presented new possibilities for analysis and understanding of home-range characteristics and created a resurgence of interest in home-range behavior and its biological significance.

Current Contents / Agriculture, Biology & Environm↗

Report of Committee on Runoff, 1942–43

The Committee on Runoff was not formally constituted until February 6, 1943, when the members of the Section, as listed above, were asked to serve. At the suggestion of President CHURCH the Committee has been so selected that there is Nation‐wide geographic distribution from West to East with the majority of the Committee composed of younger men. If the 33 papers prepared for discussion at the regular sessions of the Section of Hydrology at the annual meeting of 1943 can be used as a measure, war instead of curtailing the activities of the Section has acted as an impetus. Also in the field of hydrology as a whole, war activities have apparently not resulted in a decrease of activities. There has been, however, a gradual decrease of activities in the field of research and an increase of activities in the field of applied hydrology. The enormous expansion of our industrial machine and the great concentration of armies and industrial workers into restricted areas; the demands for water, for power, for food and for municipal use; and operations underlying many of our war efforts, secret and otherwise—all these have created problems requiring the full‐time effort of hydrologists, both in private and governmental service. It is with some degree of satisfaction that each one of us can feel that either as a result of our past research or in our present positions we have been or are doing our bit to win the war.

Eos, Transactions, American Geophysical Union↗

Modeling downstream fining in sand-bed rivers. I: Formulation

In this paper a numerical modeling formulation is presented for simulation of the development of the longitudinal profile and bed sediment distribution in sand-bed rivers. The objective of the model application, which is presented in the companion paper (Wright and Parker, 2005), is to study the development of two characteristics of large, low-slope, sand-bed rivers: (1) a downstream decrease in bed slope (i.e. concave upward longitudinal profile) and (2) a downstream decrease in characteristic bed sediment diameter (e.g. the median bed surface size D 50 ). Three mechanisms that lead to an upward concave profile and downstream fining are included in the modeling formulation: (1) a delta prograding into standing water at the downstream boundary, (2) sea-level rise, and (3) tectonic subsidence. In the companion paper (Wright and Parker, 2005) the model is applied to simulate the development of the longitudinal profile and downstream fining in sand-bed rivers flowing into the ocean during the past 5000 years of relatively slow sea-level rise.

Journal of Hydraulic Research↗

Measuring settling velocity in a strongly tidal estuary

Predicting sediment transport in estuarine systems requires understanding sediment settling velocity, its range of fluctuations, and the shortcomings of the tools to measure it. Previous studies have used Laser In-Situ Scattering and Transmissometry (LISST) instruments to measure particle size and Acoustic Doppler Velocimeters (ADV) to return estimates of settling velocity. We deployed both instruments over a 4-week period in summer 2016 in San Pablo Bay, CA, and observed conflicting results from the two instruments. The LISST indicated the settling velocity decreased with increasing bed shear stress, while the ADV showed that settling velocity increased. Comparing the experimental conditions from this study with previous research reveals that the ADV method requires steady state conditions that were not achieved. Although the ADV method uses easily measured quantities to return the settling velocity, it relies on assumptions that are not always valid in estuarine settings.

California↗

Propagation of captive American kestrels

A colony of kestrels ( Palco sparverius ) was established at the Patuxent Wildlife Research Center in 1964 in connection with work on pesticides. The kestrels were acquired from the wild, both as nestlings and as full-grown birds, and were housed in several rows of outdoor pens. Each 50 x 20 ft pen was covered with wire netting and had its long sides in common with adjacent pens. During the first two reproductive seasons, untreated parent birds (dosed birds are not included in this paper) ate eggs and young. Cannibalism virtually ceased after the diet was changed from ground beef or horsemeat supplemented with liver, vitamins, and minerals to one containing a finely ground mixture of laboratory rodents, chicken heads, skinned chicken necks, and supplements; hatching success thereafter generally equaled that of a wild population. In 1967, 16 pairs of untreated hawks (3-year-old females) laid clutches averaging 4.9 eggs, hatched 88 percent of their eggs, and fledged 88 percent of their young. In 1968, 10 pairs of this group (4-year-old females) laid clutches averaging 4.9 eggs, hatched 51 percent of their eggs, and fledged 85 percent of their young. Nine yearling pairs (hatched in captivity) laid clutches in 1968 averaging 5.1 eggs, hatched 87 percent of their eggs, and fledged all of their young.

Maryland↗

Improving scientific communication through the use of U.S. Geological Survey Video Podcasts

It is crucial that scientist find innovative ways of effectively communicating research to resource managers, public officials, and the general public. New technologies, such as video podcasts, are being used as an outreach tool to communicate results from the U.S Geological Survey (USGS) National Water-Quality Assessment (NAWQA) program. The purpose of these podcasts is to summarize scientific research and methods from the NAWQA program. Video podcasts are audio podcasts that incorporate video clips to illustrate ideas presented in simple, concise language with brief 3 to 5-minute films. The process of creating concise podcast messages expands the potential audience for communicating research findings, but the production of video podcasts requires adequate allocation of time and resources. Audience responses to NAWQA podcasts thus far indicate that video is an effective means of sharing scientific information with a broader audience.

Conference Paper↗

A simplified slope-area method for estimating flood discharges in natural channels

Discharge of a stream may be computed from the slope of the water surface, the cross-sectional area, and an estimate of channel roughness. This, the slope-area method, is widely used to compute flood peak discharges from high-water marks. Reliability of a computed discharge depends largely on the roughness coefficient, which must be estimated. This paper shows that results of comparable accuracy can be obtained from area and slope alone in natural channels; a roughness coefficient is not needed because roughness and slope are related. The estimating equation and suggestions for application of the simplified method are included.

Journal of Research of the U.S. Geological Survey↗

The potential roles of biological soil crusts in dryland hydrologic cycles

Biological soil crusts (BSCs) are the dominant living cover in many drylands of the world. They possess many features that can influence different aspects of local hydrologic cycles, including soil porosity, absorptivity, roughness, aggregate stability, texture, pore formation, and water retention. The influence of biological soil crusts on these factors depends on their internal and external structure, which varies with climate, soil, and disturbance history. This paper presents the different types of biological soil crusts, discusses how crust type likely influences various aspects of the hydrologic cycle, and reviews what is known and not known about the influence of biological crusts on sediment production and water infiltration versus runoff in various drylands around the world. Most studies examining the effect of biological soil crusts on local hydrology are done by comparing undisturbed sites with those recently disturbed by the researchers. Unfortunately, this greatly complicates interpretation of the results. Applied disturbances alter many soil features such as soil texture, roughness, aggregate stability, physical crusting, porosity, and bulk density in ways that would not necessarily be the same if crusts were not naturally present. Combined, these studies show little agreement on how biological crusts affect water infiltration or runoff. However, when studies are separated by biological crust type and utilize naturally occurring differences among these types, results indicate that biological crusts in hyperarid regions reduce infiltration and increase runoff, have mixed effects in and regions, and increase infiltration and reduce runoff in semiarid cool and cold drylands. However, more studies are needed before broad generalizations can be made on how biological crusts affect infiltration and runoff. We especially need studies that control for sub-surface soil features such as bulk density, micro- and macropores, and biological crust structure. Unlike the mixed effects of biological crusts on infiltration and runoff among regions, almost all studies show that biological crusts reduce sediment production, regardless of crust or dryland type.

Hydrological Processes↗

Moving environmental DNA methods from concept to practice for monitoring aquatic macroorganisms

The discovery that macroorganisms can be detected from their environmental DNA (eDNA) in aquatic systems has immense potential for the conservation of biological diversity. This special issue contains 11 papers that review and advance the field of eDNA detection of vertebrates and other macroorganisms, including studies of eDNA production, transport, and degradation; sample collection and processing to maximize detection rates; and applications of eDNA for conservation using citizen scientists. This body of work is an important contribution to the ongoing efforts to take eDNA detection of macroorganisms from technical breakthrough to established, reliable method that can be used in survey, monitoring, and research applications worldwide. While the rapid advances in this field are remarkable, important challenges remain, including consensus on best practices for collection and analysis, understanding of eDNA diffusion and transport, and avoidance of inhibition in sample collection and processing. Nonetheless, as demonstrated in this special issue, eDNA techniques for research and monitoring are beginning to realize their potential for contributing to the conservation of biodiversity globally.

Biological Conservation↗