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Forest rodents provide directed dispersal of Jeffrey pine seeds

Some species of animals provide directed dispersal of plant seeds by transporting them nonrandomly to microsites where their chances of producing healthy seedlings are enhanced. We investigated whether this mutualistic interaction occurs between granivorous rodents and Jeffrey pine ( Pinus jeffreyi ) in the eastern Sierra Nevada by comparing the effectiveness of random abiotic seed dispersal with the dispersal performed by four species of rodents: deer mice ( Peromyscus maniculatus ), yellow-pine and long-eared chipmunks ( Tamias amoenus and T. quadrimaculatus ), and golden-mantled ground squirrels ( Spermophilus lateralis ). We conducted two caching studies using radio-labeled seeds, the first with individual animals in field enclosures and the second with a community of rodents in open forest. We used artificial caches to compare the fates of seeds placed at the range of microsites and depths used by animals with the fates of seeds dispersed abiotically. Finally, we examined the distribution and survival of naturally establishing seedlings over an eight-year period. Several lines of evidence suggested that this community of rodents provided directed dispersal. Animals preferred to cache seeds in microsites that were favorable for emergence or survival of seedlings and avoided caching in microsites in which seedlings fared worst. Seeds buried at depths typical of animal caches (5–25 mm) produced at least five times more seedlings than did seeds on the forest floor. The four species of rodents differed in the quality of dispersal they provided. Small, shallow caches made by deer mice most resembled seeds dispersed by abiotic processes, whereas many of the large caches made by ground squirrels were buried too deeply for successful emergence of seedlings. Chipmunks made the greatest number of caches within the range of depths and microsites favorable for establishment of pine seedlings. Directed dispersal is an important element of the population dynamics of Jeffrey pine, a dominant tree species in the eastern Sierra Nevada. Quantifying the occurrence and dynamics of directed dispersal in this and other cases will contribute to better understanding of mutualistic coevolution of plants and animals and to more effective management of ecosystems in which directed dispersal is a keystone process.

Ecology↗

1.13 – Emerging contaminants

Since the Industrial Revolution, a diversity of large-scale chemical innovations has impacted aquatic systems in urban environments. Beginning in the 1990s, there has been a growing scientific interest and public awareness of the effects of the chemicals used in domestic, commercial, industrial, and agricultural applications, referred to in this article as ‘emerging contaminants’ (ECs), on ecosystem and human health. The growing global population and its increasing demands on water supplies in conjunction with climate-induced changes in hydrologic regimes place stress on freshwater resources, resulting in a greater reliance on reuse of reclaimed municipal wastewater treatment plant (WWTP) effluents to meet human and environmental needs. WWTP effluents are a major source of ECs, and it is important to have an understanding of the chemical composition of the reclaimed water, because many ECs are biologically active and the effects of chronic exposure to low concentration complex mixtures are unknown. Several classes of ECs that have been shown to be widespread in the aquatic environment are discussed in this chapter, including surfactants, complexing agents, fragrances, antimicrobials, industrial chemicals, pharmaceuticals, natural and synthetic estrogens, and disinfection byproducts. All of these compounds are biologically active via a variety of modes of action, and can occur in aquatic systems at concentrations ranging from <0.001 to >100 μg l −1 .

Book chapter↗

Concentrations and size distribution of TiO2 and Ag engineered particles in five wastewater treatment plants in the United States

The growing use of engineered particles ( e.g. , nanosized and pigment sized particles, 1 to 100 nm and 100 to 300 nm, respectively) in a variety of consumer products increases the likelihood of their release into the environment. Wastewater treatment plants (WWTPs) are an important pathways of introduction of engineered particles to the aquatic systems. This study reports the concentrations, removal efficiencies, and particle size distributions of Ag and TiO 2 engineered particles in five WWTPs in three states in the United States. The concentration of Ag engineered particles was quantified as the total Ag concentration, whereas the concentration of TiO 2 engineered particles was quantified using mass-balance calculations and shifts in the elemental ratio of Ti/Nb above their natural background elemental ratio. Ratios of Ti/Nb in all WWTP influents, activated sludges, and effluents were 2–12 times higher ( e.g. , 519 to 3243) than the natural background Ti/Nb ratio ( e.g. , 267 ± 9), indicating that 49–92% of Ti originates from anthropogenic sources. The concentration of TiO 2 engineered particles (in μg TiO 2 L −1 ) in the influent, activated sludge, and effluent varied within the ranges of 70–670, 3570–6700, and 7–30, respectively. The concentration of Ag engineered particles (in μg Ag L −1 ) in the influent, activated sludge, and effluent varied within the ranges of 0.11–0.33, 1.45–1.65, and 0.01–0.04, respectively. The overall removal efficiency ( e.g. , effluent/influent concentrations) of TiO 2 engineered particles ( e.g. , 90 to 96%) was higher than that for Ag engineered particles ( e.g. , 82 to 95%). Particles entering WWTPs are in the nanosized range for Ag ( e.g. , >99%) and a mixture of nanosized ( e.g. , 15 to 90%) and pigment sized particles ( e.g. , 10 to 85%) for TiO 2 . Nearly all Ag (>99%) and 55 to 100% of TiO 2 particles discharged to surface water with WWTP effluent are within the nanosize range. This study provides evidence that TiO 2 and Ag engineered nanomaterials enter aquatic systems with WWTP effluents, and that their concentrations are expected to increase with the increased applications of TiO 2 and Ag engineered nanomaterials in consumer products.

California, Massachusetts, South Carolina↗

Palaeobotanical evidence for a June 'impact winter' at the Cretaceous/Tertiary boundary

A large bolide impact, such as that thought to have occurred at the Cretaceous/Tertiary (K/T) boundary, should produce large amounts of light-attenuating debris, thereby causing an 'impact winter' 1–3 . Because of thermal buffering in the oceans, evidence for a brief (1–2 months 2–4 ) impact winter would be found only in terrestrial environments. Aquatic leaves in the K/T boundary section near Teapot Dome, Wyoming, preserve structural deformation that can be duplicated experimentally in extant aquatic leaves by freezing. Reproductive stages reached by the fossil aquatic plants at the time of death suggest that freezing took place in approximately early June. Both the existence of the structurally deformed plants and the high abundance of fern spores occur in a horizon containing sparse impact debris, but below the horizon containing abundant impact debris; I therefore suggest that the lower horizon represents debris and effects from a large, distant bolide impact, and the upper horizon represents a small, nearby bolide impact.

Nature↗

Born of fire - restoring sagebrush steppe

Fire is a natural feature of sagebrush grasslands in the Great Basin. The invasion of exotic annual grasses, such as Bromus tectorum (cheatgrass), has changed the environment in these ecosystems. Invasive annual grasses provide a dense and continuous source of fuel that extends the season for fires and increases the frequency of fires in the region. Frequent fires eventually eliminate the native sagebrush. These annual grasses also change soil nutrients, especially carbon and nitrogen, such that invasive annual grasses are favored over the native plants. The Forest and Rangeland Ecosystem Science Center of the U.S. Geological Survey (USGS) is studying how to reduce the problems caused by these invasive annual grasses and restore native sagebrush grasslands. The areas of research include understanding disturbance regimes, especially fire, discerning the role of nutrients in restoring native plants, determining the potential to restore forbs important for wildlife, and ascertaining the past and present use of native and nonnative plants in revegetation projects.

Fact Sheet↗

Encroachment of oriental bittersweet into Pitcher’s thistle habitat

Common invasive species and rare endemic species can grow and interact at the ecotone between forested and non-forested dune habitats. To investigate these interactions, a comparison of the proximity and community associates of a sympatric invasive ( Celastrus orbiculatus ; oriental bittersweet) and native ( C. scandens ; American bittersweet) liana species to federally threatened Cirsium pitcheri (Pitcher's thistle) in the dunes habitats of Lake Michigan was conducted. Overall, the density of the invasive liana species was significantly greater in proximity to C. pitcheri than the native species. On the basis of composition, the three focal species occurred in both foredune and blowout habitats. The plant communities associated with the three focal species overlapped in ordination space, but there were significant differences in composition. The ability of C. orbiculatus to rapidly grow and change the ecological dynamics of invasion sites adds an additional threat to the successional habitats of C. pitcheri .

Lake Michigan↗

Monitoring and research at Walnut Creek National Wildlife Refuge

Walnut Creek National Wildlife Refuge-Prairie Learning Center (Walnut Creek or the Refuge) is one of the newest additions to the National Wildlife Refuge System, which consists of over 480 units throughout the United States operated by the U.S. Department of the Interior, Fish and Wildlife Service (the Service). Located about 20 miles east of Des Moines, Iowa, the Refuge has an approved acquisition boundary containing 8,654 acres (Figure 1). Acquisition is from willing sellers only, and to date the Service has purchased approximately 5,000 acres. The acquisition boundary encompasses about 43% of the watershed of Walnut Creek, which bisects the Refuge and drains into the Des Moines River to the southeast. Approximately 25%-30% of the Walnut Creek watershed is downstream of the Refuge. As authorized by Congress in 1990, the purposes of the Refuge are to (U.S. Fish and Wildlife Service 1992): • restore native tallgrass pairie, wetland, and woodland habitats for breeding and migratory waterfowl and resident wildlife; • serve as a major environmental education center providing opportunities for study; • provide outdoor recreation benefits to the public; and • provide assistance to local landowners to improve their lands for wildlife habitat. To implement these purposes authorized by Congress, the Refuge has established the goal of recreating as nearly as possible the natural communities that existed at the time of settlement by Euro-Americans (circa 1840). Current land use is largely agricultural, including 69% cropland, 17% grazed pasture, and 7.5% grassland (dominantly brome) enrolled in the Conservation Reserve Program). About 1,395 acres of relict native communities also exist on the Refuge, including prairie (725 acres), oak savanna and woodland (450 acres), and riparian or wetland areas (220 acres). Some of these relicts are highly restorable; others contain only a few prairie plants in a matrix of brome and will be more difficult to restore. When the process of restoration (management and enhancement of existing remnant communities) and reconstruction (reestablishment of native communities on agricultural sites) is completed, it is anticipated that the Refuge will consist of approximately 5,900 acres of prairie, 2,000 acres of oak savanna or woodland, and 750 acres of wetland and riparian communities. Ecological restoration and reconstruction at this scale in the tallgrass prairie ecosystem is unprecedented in the United States.

Report↗

Colocating artificial intelligence data centers with energy infrastructure on Federal public lands—A science synthesis and spatial analysis to inform decision making

Executive Summary Artificial intelligence (AI) is rapidly transforming industries and economies, creating an urgent need to strategically plan for the energy and infrastructure required to support increasing AI use. U.S. Federal agencies and bureaus have been directed to explore ways to accelerate permitting, development, and deployment of energy resources and AI technologies, including encouraging the colocation of energy infrastructure and data centers. To inform these initiatives, this report synthesizes relevant scientific information and presents a spatial analysis of existing energy infrastructure and data centers on or near U.S. Federal public lands managed by the Bureau of Land Management (BLM). The purpose of this science synthesis and spatial analysis is to provide the BLM with foundational information for considering potential colocation of data centers with energy infrastructure on Federal public lands to support evidence-based decisions. Additionally, this report provides insight into current (2025) and potential future energy demands by providing projections of a range of potential future environmental conditions relevant to maintaining industry-recommended cooling temperature standards necessary for efficient data center operations. As a part of this effort, a rapid response literature review was conducted of the best available science on the topic of data center development and energy infrastructure in July–August 2025, supplemented by additional resources recommended by U.S. Federal agency and bureau subject matter experts (hereafter experts; including the U.S. Department of Energy National Laboratory of the Rockies) and peer reviewers. To better understand current conditions relevant to AI data center development, a spatial analysis was conducted across Alaska and 11 States in the Western United States, Arizona, California, Colorado, Idaho, Montana, Nevada, New Mexico, Oregon, Utah, Washington, and Wyoming, all of which contain extensive BLM-managed surface lands (hereafter referred to as “BLM lands”) that could be considered for the colocation of energy infrastructure and AI data centers. This effort identified BLM lands within 10 miles of existing transmission lines, consistent with methods used in previous BLM programmatic environmental impact statements. This report describes the types of data centers operating within the United States, which vary in ownership, size, technology, and proximity to end users. This report then outlines the primary considerations of data center development, including reliable energy supply, natural resources (such as water availability to support cooling requirements), and relevant policy and regulatory considerations. Energy supply considerations are pivotal for data center operation. Between 2014 and 2018, data centers in the United States accounted for nearly 2 percent of the Nation’s total electricity consumption, and data center energy consumption is projected to increase from 2 to 6.7–12 percent of total U.S. electricity use by 2028. These energy requirements necessitate careful consideration of energy supply when considering potentially suitable locations for data center development. Experts anticipate that an increase in renewable energy generation will likely support most potential future power demand needs, including for data centers, followed by increases in natural gas, nuclear, and geothermal energy production. Additional capacity in the form of battery storage will likely not generate electricity, but may improve the reliability and flexibility of supply, helping to ensure that growing data center loads can be met. However, the U.S. Department of Energy estimates that the United States will need, on average, 57 percent more energy transmission infrastructure by 2035 to account for the growing power demand introduced by development such as data centers. Cooling server equipment in data centers requires large amounts of electricity and water, and this demand can be exacerbated by hot and humid conditions. Energy efficient water-based cooling technologies may reduce electricity consumption onsite but require more water consumption. This additional water demand has the potential to increase water stress and competition with other users. As such, developing data centers will likely need a thorough assessment of current and potential future water availability, as well as consideration of how water demand may change across other sectors. Data center development involves policy and regulatory considerations, as projects must undergo environmental review and authorization processes that can take 18–24 months. Coordinating these environmental reviews and authorizations with other energy development projects, such as building new transmission lines, may cause additional delays. Recent efforts by the U.S. Department of Energy and U.S. Department of the Interior aim to expedite environmental reviews and authorizations and improve coordination across agencies. The spatial analysis identified 771 existing AI data centers and more than 3,300 power plants. The spatial analysis found that 6 percent of AI data centers and 22 percent of power plants in the Western United States were on or within 1 mile of BLM lands, and California had the largest number of facilities. Most existing AI data centers were near high-voltage transmission lines and close to power plants, supporting efficient energy delivery. More than 90,000,000 acres of BLM lands were within 10 miles of existing high-voltage transmission lines, representing 38 percent of BLM lands in the study area. Available transmission infrastructure and the overlap with BLM lands varied by State, and Alaska had limited overlap compared to the rest of the Western United States. To operate most efficiently, data center temperatures must be at or below 80.6 degrees Fahrenheit. This analysis of future temperature and precipitation projections indicated increasing cooling demands for data centers, particularly in Arizona, California, and Nevada, where rising temperatures are expected to increase energy and operational costs while potentially stressing current regional electrical grid infrastructure. This report highlights relevant energy supply, natural resources, and regulatory considerations for data center development on BLM lands. This report does not provide a comprehensive ecological, regulatory, land suitability, or permitting analysis. The factors described here are contextual considerations only and are not intended to identify, rank, quantify, or recommend optimal areas for data center colocation. This spatial analysis focused solely on energy considerations relevant to data centers and did not consider water availability, critical habitats, BLM National Conservation Lands, areas of cultural or historical significance, and other sensitive resources. These topics are recognized as critical but were not within the scope of this science synthesis and spatial analysis.

Scientific Investigations Report↗

Alternative lifestyles: A plague persistence hypothesis

Several explanations have been posited for how the plague bacterium ( Yersinia pestis ) reemerges during sylvatic cycles within the same foci over many years, and often without direct evidence of host die-offs. One prevalent view is that transmission-optimized Y. pestis bacteria, exhibiting epizootic/enzootic behavior, almost continually replicate and survive through repeated, linked, host-centered propagation events. These bacteria, we will refer to as “ r-pestis ” type ecotype(s), represent a limited number of phenotypic lineages exhibiting optimal transmissibility and high rates of reproduction. These attributes, it is thought, assure their durability through time. For continuous r-pestis type expansions to be successful, adequate numbers of fleas and hosts must become infected to produce massive numbers of bacteria. In the process, host and flea numbers decline as they succumb to plague. Here we hypothesize that r-pestis population expansions seed the environment and confront a unique, highly competitive local milieu, where natural selection favors new ecotypes that incorporate a range of emergent adaptive survival strategies. These newly adapted survivors we recognize as a range of “ K - pestis ” ecotypes with greater durability and lower reproduction rates. These emergent K - pestis forms may arise in succession or coexist for varying periods of time with r-pestis ecotypes, and with other K - pestis ecotypes. Among K-pestis ecotypes, we hypothesize that through adaptive radiations, some persist within flea life stages, soil, organic waste, amoebae, plants, carcasses, hosts, or within niches yet to be characterized. In some settings, after a long quiet period, when favorable, K-pestis bacteria may trigger a singular event where an r-pestis transmission stream emerges precipitating another enzootic/epizootic progression. If this hypothesis withstands rigorous testing, then Y. pestis might represent an even more formidable, enduring, and adaptable foe, where unforeseen local events could trigger new epidemic and epizootic/enzootic events threatening humans and populations of other mammals, including those of conservation concern.

Ecosphere↗

Restoration handbook for sagebrush steppe ecosystems with emphasis on greater sage-grouse habitat—Part 1. Concepts for understanding and applying restoration

Sagebrush steppe ecosystems in the United States currently occur on only about one-half of their historical land area because of changes in land use, urban growth, and degradation of land, including invasions of non-native plants. The existence of many animal species depends on the existence of sagebrush steppe habitat. The greater sage-grouse ( Centrocercus urophasianus ) is a landscape-dependent bird that requires intact habitat and combinations of sagebrush and perennial grasses to exist. In addition, other sagebrush-obligate animals also have similar requirements and restoration of landscapes for greater sage-grouse also will benefit these animals. Once sagebrush lands are degraded, they may require restoration actions to make those lands viable habitat for supporting sagebrushobligate animals. This restoration handbook is the first in a three-part series on restoration of sagebrush ecosystems. In Part 1, we discuss concepts surrounding landscape and restoration ecology of sagebrush ecosystems and greater sage-grouse that habitat managers and restoration practitioners need to know to make informed decisions regarding where and how to restore specific areas. We will describe the plant dynamics of sagebrush steppe ecosystems and their responses to major disturbances, fire, and defoliation. We will introduce the concepts of ecosystem resilience to disturbances and resistance to invasions of annual grasses within sagebrush steppe. An introduction to soils and ecological site information will provide insights into the specific plants that can be restored in a location. Soil temperature and moisture regimes are described as a tool for determining resilience and resistance and the potential for various restoration actions. Greater sage-grouse are considered landscape birds that require large areas of intact sagebrush steppe; therefore, we describe concepts of landscape ecology that aid our decisions regarding habitat restoration. We provide a brief overview of restoration techniques for sage-grouse habitat restoration. We conclude with a description of the critical nature of monitoring for adaptive management of sagebrush steppe restoration at landscape- and project-specific levels.

Arizona, California, Colorado, Idaho, Montana, Neb↗

Probabilistic estimation of dune retreat on the Gold Coast, Australia

Sand dunes are an important natural buffer between storm impacts and development backing the beach on the Gold Coast of Queensland, Australia. The ability to forecast dune erosion at a prediction horizon of days to a week would allow efficient and timely response to dune erosion in this highly populated area. Towards this goal, we modified an existing probabilistic dune erosion model for use on the Gold Coast. The original model was trained using observations of dune response from Hurricane Ivan on Santa Rosa Island, Florida, USA (Plant and Stockdon 2012. Probabilistic prediction of barrier-island response to hurricanes, Journal of Geophysical Research, 117(F3), F03015). The model relates dune position change to pre-storm dune elevations, dune widths, and beach widths, along with storm surge and run-up using a Bayesian network. The Bayesian approach captures the uncertainty of inputs and predictions through the conditional probabilities between variables. Three versions of the barrier island response Bayesian network were tested for use on the Gold Coast. One network has the same structure as the original and was trained with the Santa Rosa Island data. The second network has a modified design and was trained using only pre- and post-storm data from 1988-2009 for the Gold Coast. The third version of the network has the same design as the second version of the network and was trained with the combined data from the Gold Coast and Santa Rosa Island. The two networks modified for use on the Gold Coast hindcast dune retreat with equal accuracy. Both networks explained 60% of the observed dune retreat variance, which is comparable to the skill observed by Plant and Stockdon (2012) in the initial Bayesian network application at Santa Rosa Island. The new networks improved predictions relative to application of the original network on the Gold Coast. Dune width was the most important morphologic variable in hindcasting dune retreat, while hydrodynamic variables, surge and run-up elevation, were also important

Queensland↗

Predicting large hydrothermal systems

We train five models using two machine learning (ML) regression algorithms (i.e., linear regression and XGBoost) to predict hydrothermal upflow in the Great Basin. Feature data are extracted from datasets supporting the INnovative Geothermal Exploration through Novel Investigations Of Undiscovered Systems project (INGENIOUS). The label data (the reported convective signals) are extracted from measured thermal gradients in wells by comparing the total estimated heat flow at the wells to the modeled background conductive heat flow. That is, the reported convective signal is the difference between the background conductive heat flow and the well heat flow. The reported convective signals contain outliers that may affect upflow prediction, so the influence of outliers is tested by constructing models for two cases: 1) using all the data (i.e., -91 to 11,105 mW/m2), and 2) truncating the range of labels to include only reported convective signals between -25 and 200 mW/m2. Because hydrothermal systems are sparse, models that predict high convective signal in smaller areas better match the natural frequency of hydrothermal systems. Early results demonstrate that XGBoost outperforms linear regression. For XGBoost using the truncated range of labels, half of the high reported signals are within < 3 % of the highest predictions. For XGBoost using the entire range of labels, half of the high reported signals are in < 13 % of the highest predictions. While this implies that the truncated regression is superior, the all-data model better predicts the locations of power-producing systems (i.e., the operating power plants are in a smaller fraction of the study area given by the highest predictions). Even though the models generally predict greater hydrothermal upflow for higher reported convective signals than for lower reported convective signals, both XGBoost models consistently underpredict the magnitude of higher signals. This behavior is attributed to low resolution/granularity of input features compared with the scale of a hydrothermal upflow zone (a few km or less across). Trouble estimating exact values while still reliably predicting high versus low convective signals suggests that a future strategy such as ranked ordinal regression (e.g., classifying into ordered bins for low, medium, high, and very high convective signal) might fit better models, since doing so reduces problems introduced by outliers while preserving the property of larger versus smaller signals.

Geothermal Resources Council Transactions↗

Occurrence, temporal variation, and estrogenic burden of five parabens in sewage sludge collected across the United States

Five parabens used as preservatives in pharmaceuticals and personal care products (PPCPs) were measured in sewage sludges collected at 14 U.S. wastewater treatment plants (WWTPs) located in nine states. Detected concentration ranges (ng/g, dry weight) and frequencies were as follows: methyl paraben (15.9 to 203.0; 100%), propyl paraben (0.5 to 7.7; 100%), ethyl paraben (< 0.6 to 2.6; 63%), butyl paraben (< 0.4 to 4.3; 42%) and benzyl paraben (< 0.4 to 3.3; 26%). The estrogenicity inherent to the sum of parabens detected in sewage sludge (ranging from 10.1 to 500.1 pg/kg 17 β- estradiol equivalents) was insignificant when compared to the 10 6 -times higher value calculated for natural estrogens reported in the literature to occur in sewage sludge. Temporal monitoring at one WWTP provided insights into temporal and seasonal variations in paraben concentrations. This is the first report on the occurrence of five parabens in sewage sludges from across the U.S., and internationally, the first on temporal variations of paraben levels in sewage sludge. Study results will help to inform the risk assessment of sewage sludge destined for land application (biosolids).

Arizona, Indiana, Florida, Maryland, Montana, New ↗

Cloud cover and delayed herbivory relative to timing of spring onset interact to dampen climate change impacts on net ecosystem exchange in a coastal Alaskan wetland

Rapid warming in northern ecosystems over the past four decades has resulted in earlier spring, increased precipitation, and altered timing of plant–animal interactions, such as herbivory. Advanced spring phenology can lead to longer growing seasons and increased carbon (C) uptake. Greater precipitation coincides with greater cloud cover possibly suppressing photosynthesis. Timing of herbivory relative to spring phenology influences plant biomass. None of these changes are mutually exclusive and their interactions could lead to unexpected consequences for Arctic ecosystem function. We examined the influence of advanced spring phenology, cloud cover, and timing of grazing on C exchange in the Yukon–Kuskokwim Delta of western Alaska for three years. We combined advancement of the growing season using passive-warming open-top chambers (OTC) with controlled timing of goose grazing (early, typical, and late season) and removal of grazing. We also monitored natural variation in incident sunlight to examine the C exchange consequences of these interacting forcings. We monitored net ecosystem exchange of C (NEE) hourly using an autochamber system. Data were used to construct daily light curves for each experimental plot and sunlight data coupled with a clear-sky model was used to quantify daily and seasonal NEE over a range of incident sunlight conditions. Cloudy days resulted in the largest suppression of NEE, reducing C uptake by approximately 2 g C m −2 d −1 regardless of the timing of the season or timing of grazing. Delaying grazing enhanced C uptake by approximately 3 g C m −2 d −1 . Advancing spring phenology reduced C uptake by approximately 1.5 g C m −2 d −1 , but only when plots were directly warmed by the OTCs; spring advancement did not have a long-term influence on NEE. Consequently, the two strongest drivers of NEE, cloud cover and grazing, can have opposing effects and thus future growing season NEE will depend on the magnitude of change in timing of grazing and incident sunlight.

Alaska↗

Plague bacterium as a transformer species in prairie dogs and the grasslands of western North America

Invasive transformer species change the character, condition, form, or nature of ecosystems and deserve considerable attention from conservation scientists. We applied the transformer species concept to the plague bacterium Yersinia pestis in western North America, where the pathogen was introduced around 1900. Y. pestis transforms grassland ecosystems by severely depleting the abundance of prairie dogs ( Cynomys spp. ) and thereby causing declines in native species abundance and diversity, including threatened and endangered species; altering food web connections; altering the import and export of nutrients; causing a loss of ecosystem resilience to encroaching invasive plants; and modifying prairie dog burrows. Y. pestis poses an important challenge to conservation biologists because it causes trophic-level perturbations that affect the stability of ecosystems. Unfortunately, understanding of the effects of Y. pestis on ecosystems is rudimentary, highlighting an acute need for continued research.

Conservation Biology↗

Using noninvasive genetics for estimating density and assessing diet of urban and rural coyotes in Florida, USA

Coyotes ( Canis latrans ) are expanding their range and due to conflicts with the public and concerns of Coyotes affecting natural resources such as game or sensitive species, there is interest and often a demand to monitor Coyote populations. A challenge to monitoring is that traditional invasive methods involving live-capture of individual animals are costly and can be controversial. Natural resource management agencies can benefit from contemporary noninvasive genetic sampling approaches aimed at determining key aspects of Coyote ecology (e.g., population density and food habits). However, the efficacy of such approaches under different environmental conditions is poorly understood. Our objectives were to 1) examine accumulation and nuclear DNA degradation rates of Coyote scats in metropolitan and rural sites in Florida to help optimize methods to estimate population density; and 2) explore new genetic methods for determining diet of Coyotes based on vertebrate, plant, and invertebrate species DNA identified in scat. Recently developed DNA metabarcoding approaches make it possible to simultaneously identify DNA from multiple prey species in predator scat samples, but an exploration of this tool for assessing Coyote diet has not been pursued. We observed that scat accumulation rates (0.02 scats/km/day) did not vary between sites and fecal DNA amplification success decreased and genotyping errors increased over time with exposure to sun and precipitation. DNA sampling allowed us to generate a Coyote density estimate for the urban environment of eight Coyotes per 100 km2, but lack of recaptures in the rural area precluded density estimation. DNA metabarcoding showed promise for assessing diet contributions of vertebrate species to Coyote diet. Feral Swine (Sus scrofa) were detected as prey at higher frequencies than previously reported. We identify several considerations that can be used to optimize future noninvasive sampling efforts for Coyotes in the southeastern United States. We also discuss strengths and drawbacks of utilizing DNA metabarcoding for assessing diet of generalist carnivores such as Coyotes.

Florida↗

Long-term changes in ground water chemistry at a phytoremediation demonstration site

A field-scale demonstration project was conducted to evaluate the capability of eastern cottonwood trees ( Populus deltoides ) to attenuate trichloroethene (TCE) contamination of ground water. By the middle of the sixth growing season, trees planted where depth to water was <3 m delivered enough dissolved organic carbon to the underlying aquifer to lower dissolved oxygen concentrations, to create iron-reducing conditions along the plume centerline and sulfate-reducing or methanogenic conditions in localized areas, and to initiate in situ reductive dechlorination of TCE. Apparent biodegradation rate constants for TCE along the centerline of the plume beneath the phytoremediation system increased from 0.0002/d to 0.02/d during the first six growing seasons. The corresponding increase in natural attenuation capacity of the aquifer along the plume centerline, from 0.0004/m to 0.024/m, is associated with a potential decrease in plume-stabilization distance from 9680 to 160 m. Demonstration results provide insight into the amount of vegetation and time that may be needed to achieve cleanup objectives at the field scale.

Ground Water↗

Effects of flow modification on a cattail wetland at the mouth of Irondequoit Creek near Rochester, New York: Water levels, wetland biota, sediment, and water quality

An 11-year (1990-2001) study of the Ellison Park wetland, a 423-acre, predominantly cattail (Typha glauca) wetland at the mouth of Irondequoit Creek, was conducted to document the effects that flow modifications, including installation of a flow-control structure (FCS) in 1997 and increased diversion of stormflows to the backwater areas of the wetland, would have on the wetland's ability to decrease chemical loads transported by Irondequoit Creek into Irondequoit Bay on Lake Ontario. The FCS was designed to raise the water-surface elevation and thereby increase the dispersal and detention of stormflows in the upstream half of the wetland; this was expected to promote sedimentation and microbial utilization of nutrients, and thereby decrease the loads of certain constituents, primarily phosphorus, that would otherwise be carried into Irondequoit Bay. An ecological monitoring program was established to document changes in the wetland's water levels, biota, sedimentation rates, and chemical quality of water and sediment that might be attributable to the flow modifications. Water-level increases during storms were mostly confined to the wetland area, within about 5,000 ft upstream from the FCS. Backwater at a point of local concern, about 13,000 ft upstream, was due to local debris jams or constriction of flow by bridges and was not attributable to the FCS. Plant surveys documented species richness, concentrations of nutrients and metals in cattail tissues, and cattail productivity. Results indicated that observed differences among survey periods and between the areas upstream and downstream from the FCS were due to seasonal changes in water levels—either during the current year or at the end of the previous year's growing season—that reflected the water-surface elevation of Lake Ontario, rather than water-level control by the FCS. Results showed no adverse effects from the naturally high water levels that prevail annually during the spring and summer in the wetland, nor from the short-duration increases in water levels that result from FCS operation. Fish surveys documented the use of the wetland by 44 species, of which 25 to 29 species were found in any given year. Community composition was relatively consistent during the study, but seasonal and year-to-year variations in dominant resident and nonresident species were noted, and probably reflected natural or regional population patterns in Lake Ontario and Irondequoit Bay. The FCS allowed fish passage at all water levels and had no discernible adverse effect on the fish community. Bird surveys documented the use of the wetland by more than 90 species for breeding, feeding, and migration. Ground-nesting birds were unaffected by the FCS. Seasonally high water levels, rather than short-duration increases caused by the FCS, might have caused the scarcity or absence of certain wetland species by limiting the extent of breeding habitat for some species and the exposure of mud flats that attracted other species. Some noticeably scarce or absent species also were rare or absent elsewhere along the south-central shore of Lake Ontario. Benthic-macroinvertebrate studies were of minimal use for evaluating the effect of the FCS because no surveys were conducted after FCS installation. The precontrol results allowed assessment of the ecological quality of the wetland on the basis of biotic indices, and generally indicated moderately to severely impaired conditions. Differences between the macroinvertebrate communities in the southern part of the wetland and those in the northern part were attributed to habitat differences, such as substrate composition, water depth, and density of submerged aquatic vegetation. Sedimentation rates in the areas upstream and downstream from the FCS increased after the flow modifications, more in the area upstream from the FCS than in the downstream area. The concurrent downstream increase and the dynamic patterns of deposition and scour indicated that although the FCS and the other flow modifications undoubtedly were major factors in the postcontrol upstream increase in sedimentation rates, other factors, such as the magnitude, frequency, and the timing (season) of peak flows, might also have contributed. Periodic analyses of sediment samples from three longterm depositional sites in the wetland documented the concentrations of major and trace elements, polycyclic aromatic hydrocarbons, and organochlorine and organophosphate compounds. The concentrations of most constituents showed no substantial fluctuation or consistent upward or downward trend during the years sampled, nor did they identify any change after FCS installation. Comparison of the measured concentrations with sediment-quality guidelines that are used to assess the ecological quality of substrate environments indicated that the wetland was moderately to severely impaired—an assessment consistent with the benthic-macroinvertebrate biotic indices. During the precontrol period (1990–96), the wetland was a sink for particulate constituents (removal efficiencies for total phosphorus and total suspended solids were 28 and 47 percent, respectively), but had little effect on conservative constituents (chloride and sulfate). The wetland was a source of orthophosphate and ammonia (removal efficiencies were -38 and -84 percent, respectively). During the postcontrol period (1997–2001), the wetland continued to be a sink for particulate constituents (removal efficiencies for total phosphorus and total suspended solids were 45 and 52 percent, respectively); the exportation of orthophosphate by the wetland decreased (by 7 percent), whereas that of ammonia increased (by about 70 percent). The outflow loads of orthophosphate and ammonia represented about 15 and 2.3 percent of total phosphorus and total nitrogen loads, respectively. Changes in the loads of conservative constituents were negligible, and the overall removal efficiencies for other constituents during the precontrol period differed from those of the postcontrol period by no more than 5.4 percent. Statistical analyses of monthly inflow and outflow loads indicated significant differences between inflow and outflow loads of most constituents during the pre- and postcontrol periods. Load data were adjusted to remove the effects of dissimilar hydrologic conditions that prevailed during the pre- and postcontrol periods, and to isolate the water-quality-improvement effect that could be attributed solely to the FCS. Results indicated that the FCS contributed significantly to the decrease in total phosphorus loads, and slightly to a decrease in ammonia-plus-organic nitrogen loads, but had little or no significant effect on loads of other constituents.

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