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Dragonfly Mercury Project—A citizen science driven approach to linking surface-water chemistry and landscape characteristics to biosentinels on a national scale

Mercury is a globally distributed pollutant that threatens human and ecosystem health. Even protected areas, such as national parks, are subjected to mercury contamination because it is delivered through atmospheric deposition, often after long-range transport. In aquatic ecosystems, certain environmental conditions can promote microbial processes that convert inorganic mercury to an organic form (methylmercury). Methylmercury biomagnifies through food webs and is a potent neurotoxicant and endocrine disruptor. The U.S. Geological Survey (USGS), the University of Maine, and the National Park Service (NPS) Air Resources Division are working in partnership at more than 50 national parks across the United States, and with citizen scientists as key participants in data collection, to develop dragonfly nymphs as biosentinels for mercury in aquatic food webs. To validate the use of these biosentinels, and gain a better understanding of the connection between biotic and abiotic pools of mercury, this project also includes collection of landscape data and surface-water chemistry including mercury, methylmercury, pH, sulfate, and dissolved organic carbon and sediment mercury concentration. Because of the wide geographic scope of the research, the project also provides a nationwide “snapshot” of mercury in primarily undeveloped watersheds.

Fact Sheet↗

Simulation of groundwater flow at the former Badger Army Ammunition Plant, Sauk County, Wisconsin

To help support remedial efforts at the former Badger Army Ammunition Plant the U.S. Geological Survey built and calibrated a transient groundwater flow model using the Newton Raphson formulation (MODFLOW–NWT) of the U.S. Geological Survey’s modular three-dimensional finite-difference code. The model simulates the groundwater flow system at the site from 1984 to 2020. The former Badger Army Ammunition Plant is a 7,275-acre site in Sauk County, Wisconsin. The plant produced smokeless gunpower and solid rocket propellent as munitions components. Peak production periods were during World War II, the Korean War, and the Vietnam War. Subsequent groundwater contamination investigations have found four plumes at the site. A health risk assessment identified at least one contaminant of concern for human health risk present in three of the plumes: the propellant burning ground plume, the deterrent burning ground plume, and the central plume. A cooperative study began between the U.S. Army Environmental Command and U.S. Geological Survey to better understand the groundwater flow system at the former Badger Army Ammunition Plant. Field data, including aquifer tests, streamflow measurements, continuous groundwater elevations, and groundwater gradients with the Wisconsin River were collected and used to inform and calibrate the groundwater flow model. The model was used to assess the variability of the groundwater system over the study period, the components of the groundwater budget, and groundwater flow directions from identified source areas towards the Wisconsin River. Model performance assessment focused on using particle tracking to compare groundwater flowpaths that originate in the contaminant source areas to the observed plume footprints. This focus on plume behavior geometry should help constrain the advective component of a future groundwater transport model of the site.

Wisconsin↗

Identifying sources of antibiotic resistance genes in the environment using the microbial Find, Inform, and Test framework

Introduction: Antimicrobial resistance (AMR) is an increasing public health concern for humans, animals, and the environment. However, the contributions of spatially distributed sources of AMR in the environment are not well defined. Methods: To identify the sources of environmental AMR, the novel microbial Find, Inform, and Test (FIT) model was applied to a panel of five antibiotic resistance-associated genes (ARGs), namely, erm(B), tet(W), qnrA, sul1, and intI1, quantified from riverbed sediment and surface water from a mixed-use region. Results: A one standard deviation increase in the modeled contributions of elevated AMR from bovine sources or land-applied waste sources [land application of biosolids, sludge, and industrial wastewater (i.e., food processing) and domestic (i.e., municipal and septage)] was associated with 34–80% and 33–77% increases in the relative abundances of the ARGs in riverbed sediment and surface water, respectively. Sources influenced environmental AMR at overland distances of up to 13 km. Discussion: Our study corroborates previous evidence of offsite migration of microbial pollution from bovine sources and newly suggests offsite migration from land-applied waste. With FIT, we estimated the distance-based influence range overland and downstream around sources to model the impact these sources may have on AMR at unsampled sites. This modeling supports targeted monitoring of AMR from sources for future exposure and risk mitigation efforts.

Wisconsin↗

Dachtal Isomers and Acidic Herbicides and Pesticides in Eggs of Osprey (Pandion haliaetus) from the Seattle and Everett Areas, Washington, U.S.A

Current-use chlorophenoxy herbicides including 2,4-dichlorophenoxyacetic acid, dicamba, triclopyr, dicamba, dimethyl tetrachloroterephthalate (DCPA or dacthal), and the metabolite of pyrethroids, 3-phenoxybenzoic acid (3-PBA), and the fungicide, chlorothalonil, were investigated in the eggs of osprey (Pandion haliaetus) that were collected from 15 sites from five study areas Puget Sound/Seattle area of Washington State, USA. DCPA differs from acidic chlorophenoxy herbicides, and is not readily hydrolyzed to free acid or acid metabolites, and thus we developed a new method. Of the 12 chlorophenoxy herbicides and chlorothalonil analyzed only DCPA could be quantified at six of these sites (2.0 to 10.3 pg/g fresh weight). However, higher levels (6.9 to 85.5 pg/g fresh weight) of the unexpected DCPA structural isomer, dimethyl tetrachlorophthalate (diMe-TCP) were quantified in eggs from all sites. diMe-TCP concentrations tended to be higher in eggs from the Everett Harbor area. As diMe-TCP is not an industrial product, and not commercially available, the source of diMe-TCP is unclear. Regardless, these findings indicate that DCPA and diMe-TCP can be accumulated in the food chain of fish-eating osprey, and transferred in ovo to eggs, and thus may be of concern to the health of the developing chick and the general reproductive health of this osprey population.

Environmental Pollution↗

Building a response network to investigate potential pathogens associated with unionid mortality events

Unexplained mortality events have confounded the mussel conservation community for over 30 years. While the effects of chemical pollutants and environmental factors have been examined, few investigations have focused on the identification of potential mussel pathogens. Consequently, very little is known regarding the impact that pathogens have on freshwater mussel health, especially as they pertain to propagation efforts. There are several barriers that have hindered progress in the study of freshwater mussel disease, one of which is lack of appropriate diagnostic tools, and another is lack of guidance for field personnel regarding sampling procedures and laboratory contacts.

Ellipsaria↗

Tire-derived transformation product 6PPD-quinone induces mortality and transcriptionally disrupts vascular permeability pathways in developing coho salmon

Urban stormwater runoff frequently contains the car tire transformation product 6PPD-quinone, which is highly toxic to juvenile and adult coho salmon ( Onchorychus kisutch ). However, it is currently unclear if embryonic stages are impacted. We addressed this by exposing developing coho salmon embryos starting at the eyed stage to three concentrations of 6PPD-quinone twice weekly until hatch. Impacts on survival and growth were assessed. Further, whole-transcriptome sequencing was performed on recently hatched alevin to address the potential mechanism of 6PPD-quinone-induced toxicity. Acute mortality was not elicited in developing coho salmon embryos at environmentally measured concentrations lethal to juveniles and adults, however, growth was inhibited. Immediately after hatching, coho salmon were sensitive to 6PPD-quinone mortality, implicating a large window of juvenile vulnerability prior to smoltification. Molecularly, 6PPD-quinone induced dose-dependent effects that implicated broad dysregulation of genomic pathways governing cell–cell contacts and endothelial permeability. These pathways are consistent with previous observations of macromolecule accumulation in the brains of coho salmon exposed to 6PPD-quinone, implicating blood–brain barrier disruption as a potential pathway for toxicity. Overall, our data suggests that developing coho salmon exposed to 6PPD-quinone are at risk for adverse health events upon hatching while indicating potential mechanism(s) of action for this highly toxic chemical.

Environmental Science & Technology↗

Sanitary quality of surface water during base-flow conditions in the Municipality of Caguas, Puerto Rico, 2014–15: A comparison with results from a similar 1997–99 study

A study was conducted in 2014–15 by the U.S. Geological Survey (USGS), in cooperation with the Municipality of Caguas, to determine if changes in the stream sanitary quality during base-flow conditions have occurred since 1997–99, when a similar study was completed by the USGS. Water samples were collected for the current study during two synoptic surveys in 2014 and 2015. Water samples were analyzed for fecal and total coliform bacteria, nitrate plus nitrite as nitrogen, nitrogen and oxygen isotopes of nitrate, and human health and pharmaceutical products. Water sampling occurred at 39 stream locations used during the 1997–99 study by the USGS and at 11 additional sites. A total of 151 stream miles were classified on the basis of fecal and total coliform bacteria results. The overall spatial pattern of the sanitary quality of surface water during 2014–15 is similar to the pattern observed in 1997–99 in relation to the standards adopted by the Puerto Rico Environmental Quality Board in 1990. Surface water at most of the water-sampling sites exceeded the current standard for fecal coliform of 200 colonies per 100 milliliters adopted by the Puerto Rico Environmental Quality Board in 2010. The poorest sanitary quality was within the urban area of the Municipality of Caguas, particularly in urban stream reaches of Río Caguitas and in rural and suburban reaches bordered by houses in high density that either have inadequate septic tanks or discharge domestic wastewater directly into the stream channels. The best sanitary quality occurred in areas having little or no human development, such as in the wards of San Salvador and Beatriz to the south and southwest of Caguas, respectively. The concentration of nitrate plus nitrite as nitrogen ranged from 0.02 to 9.0 milligrams per liter, and did not exceed the U.S. Environmental Protection Agency drinking-water standard for nitrate as nitrogen of 10 milligrams per liter. The composition of nitrogen and oxygen isotopes of nitrate indicates that the origin of nitrate in the streams is most likely animal and human waste. A baseline was established for the concentrations of selected human health and pharmaceutical products at stations in some of the streams within the Municipality of Caguas. Thirty-eight human health and pharmaceutical products were present at or above the measurement detection level.

Caguas, Puerto Rico↗

Biosafety practices when working with bats: A guide to field research considerations

Introduction: Field work with bats is an important contribution to many areas of research in environmental biology and ecology, as well as microbiology. Work with bats poses hazards such as bites and scratches, and the potential for exposure to infectious pathogens such as rabies virus. It also exposes researchers to many other potential hazards inherent to field work, such as environmental conditions, delayed emergency responses, or challenging work conditions. Methods: This article discusses the considerations for a thorough risk assessment process around field work with bats, pre- and post-occupational health considerations, and delves into specific considerations for areas related to biosafety concerns—training, personal protective equipment, safety consideration in field methods, decontamination, and waste. It also touches on related legal and ethical issues that sit outside the realm of biosafety, but which must be addressed during the planning process. Discussion: Although the focal point of this article is bat field work located in northern and central America, the principles and practices discussed here are applicable to bat work elsewhere, as well as to field work with other animal species, and should promote careful considerations of how to safely conduct field work to protect both researchers and animals.

Applied Biosafety↗

Establishment of sentinel sampling sites to monitor changes in water and sediment quality and biota related to visitor use at Lake Powell, Arizona and Utah, 2004-2006

Twenty sentinel sampling sites were established and sampled during 2004–06 at Lake Powell, Arizona and Utah, by the U.S. Geological Survey and the National Park Service—Glen Canyon National Recreation Area. The sentinel sampling sites provide sampling locations on Lake Powell, the Nation’s second largest reservoir that can be visited and sampled repeatedly over time to monitor changes in water and sediment quality and also biota. The sites were established in response to an Environmental Impact Statement that addressed the use of personal watercraft on Lake Powell. The use of personal watercraft can potentially introduce hydrocarbons and other contaminants and are of concern to the health of visitors and aquatic habitats of these environments. Data from this initial sampling period (2004–06) include (1) discrete measurements of water temperature, specific conductance, pH, and water clarity; (2) major ions, nutrients, and organic carbon; (3) trace elements including rare earths; (4) organic compounds including oil and grease, total petroleum hydrocarbons, and volatile organic compounds; (5) polycyclic aromatic hydrocarbons in lakebed sediments; and (6) continuous depth profile measurements of water temperature, specific conductance, pH, dissolved oxygen, and turbidity. Also, the National Park Service-Glen Canyon National Recreation Area collected bacteria samples during this initial sampling period.

Arizona;Utah↗

Monitoring contaminant exposure: Relative concentrations of organochlorines in three tissues of American black ducks

Comparison of organochlorine residues in wildlife must often be made to regulatory standards or to values of known biological significance; this is difficult when dissimilar tissues are analyzed and results are expressed on different bases. To relate levels in the different tissues used for regulatory and monitoring purposes and for biological assessments, we exposed American black ducks to mixtures of three organochlorines. Differences in residue burdens among tissues were not statistically significant when levels were expressed on a lipid weight basis. Levels of heptachlor and Aroclor 1254 in one tissue can be accurately predicted from those in another; such predictions for endrin are less reliable. Lipid weight based residue concentrations in wings may be used to predict whether levels in fat exceed residue standards set for human health or approach those associated with effects on populations of species sampled.

Environmental Monitoring and Assessment↗

Characterizing the areal extent of PFAS contamination in fish species downgradient of AFFF source zones

Most monitoring programs next to large per- and polyfluoroalkyl substances (PFAS) sources focus on drinking water contamination near source zones. However, less is understood about how these sources affect downgradient hydrological systems and food webs. Here, we report paired PFAS measurements in water, sediment, and aquatic biota along a hydrological gradient away from source zones contaminated by the use of legacy aqueous film-forming foam (AFFF) manufactured using electrochemical fluorination. Clustering analysis indicates that the PFAS composition characteristic of AFFF is detectable in water and fishes >8 km from the source. Concentrations of 38 targeted PFAS and extractable organofluorine (EOF) decreased in fishes downgradient of the AFFF-contaminated source zones. However, PFAS concentrations remained above consumption limits at all locations within the affected watershed. Perfluoroalkyl sulfonamide precursors accounted for approximately half of targeted PFAS in fish tissues, which explain >90% of EOF across all sampling locations. Suspect screening analyses revealed the presence of a polyfluoroketone pharmaceutical in fish species, and a fluorinated agrochemical in water that likely does not accumulate in biological tissues, suggesting the presence of diffuse sources such as septic system and agrochemical inputs throughout the watershed in addition to AFFF contamination. Based on these results, monitoring programs that consider all hydrologically connected regions within watersheds affected by large PFAS sources would help ensure public health protection.

Massachusetts↗

Atmospherically deposited PBDEs, pesticides, PCBs, and PAHs in western U.S. National Park fish: Concentrations and consumption guidelines

Concentrations of polybrominated diphenyl ethers (PBDEs), pesticides, polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons were measured in 136 fish from 14 remote lakes in 8 western U.S. National Parks/Preserves between 2003 and 2005 and compared to human and wildlife contaminant health thresholds. A sensitive (median detection limit, -18 pg/g wet weight), efficient (61% recovery at 8 ng/g), reproducible (4.1% relative standard deviation (RSD)), and accurate (7% deviation from standard reference material (SRM)) analytical method was developed and validated for these analyses. Concentrations of PCBs, hexachlorobenzene, hexachlorocyclohexanes, DDTs, and chlordanes in western U.S. fish were comparable to or lower than mountain fish recently collected from Europe, Canada, and Asia. Dieldrin and PBDE concentrations were higher than recent measurements in mountain fish and Pacific Ocean salmon. Concentrations of most contaminants in western U.S. fish were 1-6 orders of magnitude below calculated recreational fishing contaminant health thresholds. However, lake average contaminant concentrations in fish exceeded subsistence fishing cancer thresholds in 8 of 14 lakes and wildlife contaminant health thresholds for piscivorous birds in 1of 14 lakes. These results indicate that atmospherically deposited organic contaminants can accumulate in high elevation fish, reaching concentrations relevant to human and wildlife health. ?? 2008 American Chemical Society.

Environmental Science & Technology↗

Suwannee river basin and estuary integrated science workshop: September 22-24, 2004 Cedar Key, Florida

In response to the growing number of environmental concerns in the mostly pristine Suwannee River Basin and the Suwannee River Estuary system, the States of Florida and Georgia, the Federal government, and other local organizations have identified the Suwannee River as an ecosystem in need of protection because of its unique biota and important water resources. Organizations with vested interests in the region formed a coalition, the Suwannee Basin Interagency Alliance (SBIA), whose goals are to promote coordination in the identification, management, and scientific knowledge of the natural resources in the basin and estuary. To date, an integrated assessment of the physical, biological, and water resources has not been completed. A holistic, multi-disciplinary approach is being pursued to address the research needs in the basin and estuary and to provide supportive data for meeting management objectives of the entire ecosystem. The USGS is well situated to focus on the larger concerns of the basin and estuary by addressing specific research questions linking water supply and quality to ecosystem function and health across county and state boundaries. A strategic plan is being prepared in cooperation with Federal, State, and local agencies to identify and implement studies to address the most compelling research issues and management questions, and to conduct fundamental environmental monitoring studies. The USGS, Suwannee River Water Management District and the Florida Marine Research Institute are co-sponsoring this scientific workshop on the Suwannee River Basin and Estuary to: Discuss current and past research findings, Identify information gaps and research priorities, and Develop an action plan for coordinated and relevant research activities in the future. This workshop builds on the highly successful basin-wide conference sponsored by the Suwannee Basin Interagency Alliance that was held three years ago in Live Oak, Florida. This years workshop will focus on identifying information needs and priorities and developing partnerships. The USGS is seeking to define the role of the USGS Florida Integrated Science Center (FISC) in conducting integrated research in the Suwannee River Basin, and to establish a cooperative program with other agencies. Participants interested in river, floodplain, springs, estuary, or basin-wide issues are encouraged to attend. Topics for this years workshop include: Water quality and geochemistry: nutrient enrichment, reduction of nutrient loading to ground water, contaminants, and land use, Hydrogeology: interactions among ground water, surface water and ecosystem, modeling, and baseline mapping, Ecosystem dynamics: structure, process, species, and habitats (estuarine, riverine, floodplain, and wetland), and Information management: data sharing, database development, geographic information system (GIS), and basin-wide models.

Open-File Report↗

Using colony monitoring devices to evaluate the impacts of land use and nutritional value of forage on honey bee health

Colony monitoring devices used to track and assess the health status of honey bees are becoming more widely available and used by both beekeepers and researchers. These devices monitor parameters relevant to colony health at frequent intervals, often approximating real time. The fine-scale record of hive condition can be further related to static or dynamic features of the landscape, such as weather, climate, colony density, land use, pesticide use, vegetation class, and forage quality. In this study, we fit commercial honey bee colonies in two apiaries with pollen traps and digital scales to monitor floral resource use, pollen quality, and honey production. One apiary was situated in low-intensity agriculture; the other in high-intensity agriculture. Pollen traps were open for 72 h every two weeks while scales recorded weight every 15 min throughout the growing season. From collected pollen, we determined forage quantity per day, species identity using DNA sequencing, pesticide residues, amino acid content, and total protein content. From scales, we determined the accumulated hive weight change over the growing season, relating to honey production and final colony weight going into winter. Hive scales may also be used to identify the occurrence of environmental pollen and nectar dearth, and track phenological changes in plant communities. We provide comparisons of device-derived data between two apiaries over the growing season and discuss the potential for employing apiary monitoring devices to infer colony health in the context of divergent agricultural land use conditions.

Agriculture↗

Ammonia and aquatic ecosystems – A review of global sources, biogeochemical cycling, and effects on fish

The purpose of this review is to better understand the full life cycle and influence of ammonia from an aquatic biology perspective. While ammonia has toxic properties in water and air, it also plays a central role in the biogeochemical nitrogen (N) cycle and regulates mechanisms of normal and abnormal fish physiology. Additionally, as the second most synthesized chemical on Earth, ammonia contributes economic value to many sectors, particularly fertilizers, energy storage, explosives, refrigerants, and plastics. But, with so many uses, industrial N 2 -fixation effectively doubles natural reactive N concentrations in the environment. The consequence is global, with excess fixed nitrogen driving degradation of soils, water, and air; intensifying eutrophication, biodiversity loss, and climate change; and creating health risks for humans, wildlife, and fisheries. Thus, the need for ammonia research in aquatic systems is growing. In response, we prepared this review to better understand the complexities and connectedness of environmental ammonia. Even the term “ammonia” has multiple meanings. So, we have clarified the nomenclature, identified units of measurement, and summarized methods to measure ammonia in water. We then discuss ammonia in the context of the N-cycle, review its role in fish physiology and mechanisms of toxicity, and integrate the effects of human N-fixation, which continuously expands ammonia's sources and uses. Ammonia is being developed as a carbon-free energy carrier with potential to increase reactive nitrogen in the environment. With this in mind, we review the global impacts of excess reactive nitrogen and consider the current monitoring and regulatory frameworks for ammonia. The presented synthesis illustrates the complex and interactive dynamics of ammonia as a plant nutrient, energy molecule, feedstock, waste product, contaminant, N-cycle participant, regulator of animal physiology, toxicant, and agent of environmental change. Few molecules are as influential as ammonia in the management and resilience of Earth's resources.

Science of the Total Environment↗

Understanding the relationship between stream metabolism and biological assemblages

Biological assemblages are commonly used for assessing stream health, but there is increased interest among the freshwater research community in incorporating measures of stream function, such as metabolism, to strengthen stream-health assessments. Presently, there is limited information about the relationships between stream metabolism and biological assemblages, along with the measurement period required to relate metabolism with stream biota. Our study assessed which environmental factors explained stream metabolism and to what degree stream metabolism and minimum dissolved oxygen (DOmin) were related to invertebrate and fish metrics in streams distributed across several regions of the United States. Furthermore, we evaluated the number of metabolism monitoring days required for maximizing the ability to detect relationships between stream metabolism and biological assemblage metrics. We sampled 17 sites distributed among reference, agricultural, and urban areas for stream metabolism, nutrients, habitat, and biological assemblages (invertebrates and fishes). Overall, sites were heterotrophic with gross primary production (GPP) and ecosystem respiration (ER) related primarily to days since last high flow, canopy cover, maximum water temperature, and total phosphorus. DOmin was related to days since last high flow, canopy cover, and maximum water temperature. We were unable to determine a clear statistical relationship between invertebrate metrics (invertebrate richness; Ephemeroptera, Plecoptera, and Trichoptera richness; and scraper-taxa richness) and GPP, ER, or DOmin. In contrast, we found that 2 fish-assemblage metrics were associated with stream metabolism and DOmin. A fish multimetric index (FMMI) was negatively correlated with GPP ( r = −0.5, p = 0.048) and positively correlated with DOmin ( r = 0.47, p = 0.06). Percentage of omnivorous fish taxa was positively correlated with GPP ( r = 0.72, p = 0.001) and ER ( r = 0.55, p = 0.02) and negatively correlated with DOmin ( r = −0.67, p = 0.003). The lack of detected relationships for most of the biological-assemblage metrics with stream metabolism may be partially due to 1 or more factors, including high variability, low sample size, limited range in metabolism values, assemblage metrics used, and geographic distribution of sites. Comparing stream-metabolism measurement periods (in days) to biological-assemblage metrics indicated that optimum correlations occurred at 2 d for DOmin, 3 d for GPP, and 14 d for ER. Although our study found limited relationships of stream metabolism and DOmin with biological assemblages, future studies should consider a larger sample size (≥30), 14-d or longer metabolism measurement period, and assessment of other taxa-specific or assemblage metrics.

Freshwater Science↗

Geochemical processes related to mined, milled, or natural metal deposits in a rapidly changing global environment

The demand for metals and raw materials, such as nickel and copper, has been projected to expand in the coming decades, driven by the global energy transition, the need for green technologies, and expanding infrastructure. Consequently, the increasing extraction and production of mining waste can have adverse impacts on surrounding environments and human health. The aim of this thematic collection is to fill critical knowledge gaps in the present-day cycles of metal(loid)s from source to larger sinks, and the effect of environmental management, anthropogenic development, and climate change. Altogether, the studies have been conducted in different natural settings around the world and comprise investigations in laterites, a soil-medicinal plant system, watersheds, and banded iron formations, among others. The geochemical applications in tracing mineralization, its secondary products, and/or potential impact on the immediate environment are highly diverse with applied tools ranging from isotope tracers to major and trace element systematics. Particularly the use of rare earth elements, their patterns and anomalies are methods employed by several studies in this collection. We summarize the findings to offer a potential future direction for the use of geochemical tracing techniques in resource exploration in the context of climate change and environmental challenges.

Geochemistry: Exploration, Environment, Analysis↗

Water balance monitoring for two bioretention gardens in Omaha, Nebraska, 2011–14

Bioretention gardens are used to help mitigate stormwater runoff in urban settings in an attempt to restore the hydrologic response of the developed land to a natural predevelopment response in which more water is infiltrated rather than routed directly to urban drainage networks. To better understand the performance of bioretention gardens in facilitating infiltration of stormwater in eastern Nebraska, the U.S. Geological Survey, in cooperation with the Douglas County Environmental Services and the Nebraska Environmental Trust, assessed the water balance of two bioretention gardens located in Omaha, Nebraska by monitoring the amount of stormwater entering and leaving the gardens. One garden is on the Douglas County Health Center campus, and the other garden is on the property of the Eastern Nebraska Office on Aging. For the Douglas County Health Center, bioretention garden performance was evaluated on the basis of volume reduction by comparing total inflow volume to total outflow volume. The bioretention garden reduced inflow volumes from a minimum of 33 percent to 100 percent (a complete reduction in inflow volume) depending on the size of the event. Although variable, the percent reduction of the inflow volume tended to decrease with increasing total event rainfall. To assess how well the garden reduces stormwater peak inflow rates, peak inflows were plotted against peak outflows measured at the bioretention garden. Only 39 of the 255 events had any overflow, indicating 100 percent peak reduction in the other events. Of those 39 events having overflow, the mean peak reduction was 63 percent. No overflow events were recorded at the bioretention garden at the Eastern Nebraska Office on Aging; therefore, data were not available for an event-based overflow analysis.Monitoring period summary of the water balance at both bio-retention gardens indicates that most of the stormwater in the bioretention gardens is stored in the subsurface. Evapotranspiration was attributed to a small percentage of the outputs on an annual basis (3 percent at Douglas County Health Center site and 5 percent at Eastern Nebraska Office onAging site), which indicates that vegetative water uptake is not a primary factor in the water budget.

Nebraska↗