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Biogenic non-crystalline U(IV) revealed as major component in uranium ore deposits

Historically, it is believed that crystalline uraninite, produced via the abiotic reduction of hexavalent uranium (U (VI) ) is the dominant reduced U species formed in low-temperature uranium roll-front ore deposits. Here we show that non-crystalline U (IV) generated through biologically mediated U (VI) reduction is the predominant U (IV) species in an undisturbed U roll-front ore deposit in Wyoming, USA. Characterization of U species revealed that the majority ( ∼ 58-89%) of U is bound as U (IV) to C-containing organic functional groups or inorganic carbonate, while uraninite and U (VI) represent only minor components. The uranium deposit exhibited mostly 238 U-enriched isotope signatures, consistent with largely biotic reduction of U (VI) to U (IV) . This finding implies that biogenic processes are more important to uranium ore genesis than previously understood. The predominance of a relatively labile form of U (IV) also provides an opportunity for a more economical and environmentally benign mining process, as well as the design of more effective post-mining restoration strategies and human health-risk assessment.

Nature Communications↗

Long-term soil monitoring at U.S. Geological Survey reference watersheds

Monitoring the environment by making repeated measurements through time is essential to evaluate and track the health of ecosystems (fig. 1). Long-term datasets produced by such monitoring are indispensable for evaluating the effectiveness of environmental legislation and for designing mitigation strategies to address environmental changes in an era when human activities are altering the environment locally and globally.

Fact Sheet↗

Investigation of the possible connection of rock and soil geochemistry to the occurrence of high rates of neurodegenerative diseases on Guam and a hypothesis for the cause of the diseases

High incidences of neurodegenerative diseases, mainly dementia, parkinsonism, and amyotrophic lateral sclerosis, occur on the island of Guam (Koerner, 1952; Kurland and Mulder, 1954). The occurrence and description of the diseases and a summary of the investigations can be found in Perl (1997). The diseases have been more prevalent along the southern coast, particularly the small villages of Umatac, Merizo, and Inarajan (Reed and Brody, 1975; Roman, 1996; and Perl, 1997) (fig. 1), and referred to as the southern villages in this report. Tertiary volcanic rocks underlie most of the southern part of the island, including these villages. The northern part of Guam, with lower incidences of the diseases, consists of carbonate rocks. Epidemiological studies beginning in the early 1950’s failed to show the cause to be genetic etiology (Plato and others, 1986; Zhang and others, 1990). In recent studies, the search for pathogenic mechanisms has shifted to environmental factors. Excesses or deficiencies of various elements from dietary sources including drinking water can have an effect on human health. These deficiencies or excesses can usually be attributed to the geochemical composition of the rocks and derived soils that underlie the area. An example is the high concentration of Se in soil associated with the occurrence of selenosis in adults (Mills, 1996). Yase (1972) suggested that the neurodegenerative diseases on Guam may be related to accumulation of trace elements such as manganese and aluminum, both of which may cause neurodegeneration. It has been suggested that a deficiency in calcium and magnesium in the soil and water along with readily available aluminum could be connected to the occurrence of the diseases (Gajdusek, 1982; Yanagihara and others, 1984; Garruto and others, 1989). Some of the studies investigated metal exposure, particularly aluminum and manganese, and deficiencies in calcium and magnesium (Garruto and others, 1984). Aluminum has been shown to have neurotoxic effects (MacDonald and Martin, 1988), and aluminum has been implicated in the pathogenesis of Alzheimer’s disease and similar dementia by Perl and others (1982). Studies of soils developed on volcanic rocks on Guam and other islands by McLachlan and others (1989) found that soils on Guam averaged 42-fold higher yield of elutable aluminum than soils developed on volcanic rocks on Jamaica or Palau. They did not detect unusually high dietary aluminum or low dietary calcium, but concluded that the soils and possibly the dusts of Guam might be a major source of aluminum entering the body of the inhabitants. This study was conducted to investigate the geochemistry of the soils and rocks of the volcanic southern part of the island of Guam, particularly in the vicinity of the three southern villages (Umatac, Merizo, and Inarajan) with high incidences of the diseases. In addition to total chemical analyses of the soils and rocks, various extractions of soils were carried out. Both excesses and deficiencies of various elements were looked for. Because soluble aluminum in the soil was shown by McLachlan and others (1989) to be unusually high, water-soluble extractions as well as sequential extractions of the soils were carried out. In addition, elements such as aluminum found in dust can traverse the nose-brain barrier in experimental animals (Sunderman, 2000) and respiratory epithelium is known to contain the highest concentration of aluminum in the human body (Tipton and others, 1957). The availability of elements, particularly aluminum from human inhalation of dust, derived from soil, was investigated. The available elements were determined by extractions of soils using a simulated lung-fluid extraction. In order to compare the results of the chemical data of rocks and soils from Guam to other rocks and soils elsewhere, samples of similar rocks and soils were collected in the western United States and similar analyses to those for the Guam samples carried out. The complete chemical analyses of the soils, rocks, and streambed sediments as well as descriptions of the methods used can be found in Miller and others (2002).

Open-File Report↗

Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results

During fall 2010 and spring 2011, a total of 119 brown bullhead ( Ameiurus nebulosus ), 136 white sucker ( Catostomus commersoni ), 73 smallmouth bass ( Micropterus dolomieu ), and 59 largemouth bass ( M. salmoides ) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in order to document potential adverse affects from exposure to complex chemical mixtures. Fish were necropsied on site, blood samples obtained, pieces of liver, spleen, kidney, gill and any abnormalities placed in fixative for histopathology. Liver samples were saved for gene expression analysis and otoliths were removed for aging. A suite of fish health indicators was developed and implemented for site comparisons and to document seasonal effects and species differences in response to environmental conditions. Organism level (grossly visible lesions, condition factor), tissue level (microscopic pathology, organosomatic indices, micronuclei, and other nuclear abnormalities), plasma factors (reproductive steroid hormones, vitellogenin), and molecular (gene expression) indicators were included. This report describes the methods and preliminary results.

Great Lakes Basin↗

Anthropogenic activities have greatly altered mangroves over the last hundred years

Mangroves not only provide ecosystem and cultural services but also contribute to the mitigation of global warming. Mangrove dynamics and their environmental responses as re-constructed from the past can inform current mangrove conservation and restoration. However, our understanding of mangrove dynamics over the past century and the impact of human activities on these ecosystems remains limited. Using the quantified mangrove-derived organic carbon (MOC) contributions of seven sediment cores, we reconstructed the historical mangrove dynamics in Yingluo Bay and the Maowei Sea in tropical China dating back to 1900. The results indicated that the natural undisturbed mangroves in Yingluo Bay flourished in response to rising temperatures. In contrast, the significantly human-disturbed mangroves in Maowei Sea experienced a marked decline. Although both areas share similar natural conditions, intense anthropogenic disturbance reversed the natural potential for mangrove growth in the Maowei Sea. To explore the global prevalence of this phenomenon, we compiled data on mangrove pollen, δ 13 C org , MOC, and mangrove area change from over 40 sites/regions worldwide, and re-constructed the natural and human-affected mangrove dynamics over the past century. Our findings indicated that, owing to the globally rising temperatures, natural undisturbed mangroves have gradually expanded as progressively more healthy forests, while human-disturbed mangroves exhibited three patterns: (1) continuous degradation, (2) flourishing-degradation, and (3) degradation-regeneration. Anthropogenic activities, such as seawall construction, aquaculture activity, agricultural expansion, logging, and urbanization have significantly reversed the natural health of mangroves such that any conservation and restoration strategy used for mangroves globally could inherently consider anthropogenic factors along with natural environmental change for better outcomes.

Global and Planetary Change↗

A spatial decision support system for coastal management: A research project at the National Wetlands Research Center of the U.S. Geological Survey

Environmental resource managers and scientists are being challenged in developing strategies to manage complex coastal systems. From an ecological perspective, there are myriad dynamic, interrelated natural and human-induced processes that affect the health and stability of coastal systems. However, the problems associated with managing coastal resources usually transcend purely ecological factors when one considers societal needs and expectations from these resources. For example, at least nine Federal, State, and local government agencies, often with widely varying responsibilities or interests, are charged with managing environmental resources and/or regulating human activities within Louisiana's coastal systems; that number may be higher in other coastal areas of the United States. In many coastal systems, a declining resource base and environmental quality combined with an expanding human population exert increased demands on those systems. This results in a number of conflicting resource management and environmental impact assessment issues. The issues include determining the most cost-effective strategies for restoring degraded natural systems, local and regional planning for future urban and commercial development in or near sensitive coastal habitats, predicting impacts from acute and chronic pollutant discharges (oil spills, fecal coliform contamination) as well as from natural hazard damages to coastal systems, and optimal partitioning of coastal resources among competing user groups (e.g., commercial and recreational fishermen). Solving such complex environmental resource management problems often involves a multidisciplinary approach, requires computerized analytical modeling abilities to manipulate large quantities of spatial-temporal data according to a defined set of objectives or constraints, and needs a mechanism to provide quick responses for dynamic resource and environmental issues. For this, a GIS-based multifunctional Spatial Decision Support System (SDSS) is being developed at the National Wetlands Research Center of the U.S. Geological Survey (formerly the Southern Science Center of the US. Fish and Wildlife Service/National Biological Service).

Louisiana↗

Generating geochemical and mineralogy distributions of soil in the conterminous United States using Bayesian hierarchical spatial models

Characterizing geochemical and mineralogical soil distributions across large spatial extents is essential for understanding mineral resources, ecosystem processes, and environmental risks. Rasters of soil geochemical distributions for the conterminous United States, however, are limited. We present a Bayesian modeling workflow and tool for generating predictive geochemical and mineralogy distribution maps for the conterminous United States using integrated nested Laplace approximation (INLA) with the stochastic partial differential equation approach. By modeling soil geostatistical data with environmental covariates (soil properties, topography, climate, and land cover), we generate predictive distributions of soil geochemistry that can be mapped or extracted for further analyses. As an example, we model the spatial distribution of trace elements in soil relevant to vertebrate health (cobalt, copper, iron, manganese, selenium, and zinc) and provide a workflow that can be used to generate and visualize predictive distributions of 39 other major and trace elements and 21 minerals of the soil survey, supporting a variety of ecological, environmental, and agricultural applications.

MethodsX↗

Estimating lithium concentrations in groundwater used as drinking water for the conterminous United States

Lithium (Li) concentrations in drinking-water supplies are not regulated in the United States; however, Li is included in the 2022 U.S. Environmental Protection Agency list of unregulated contaminants for monitoring by public water systems. Li is used pharmaceutically to treat bipolar disorder, and studies have linked its occurrence in drinking water to human-health outcomes. An extreme gradient boosting model was developed to estimate geogenic Li in drinking-water supply wells throughout the conterminous United States. The model was trained using Li measurements from ∼13,500 wells and predictor variables related to its natural occurrence in groundwater. The model predicts the probability of Li in four concentration classifications, ≤4 μg/L, >4 to ≤10 μg/L, >10 to ≤30 μg/L, and >30 μg/L. Model predictions were evaluated using wells held out from model training and with new data and have an accuracy of 47–65%. Important predictor variables include average annual precipitation, well depth, and soil geochemistry. Model predictions were mapped at a spatial resolution of 1 km 2 and represent well depths associated with public- and private-supply wells. This model was developed by hydrologists and public-health researchers to estimate Li exposure from drinking water and compare to national-scale human-health data for a better understanding of dose–response to low (<30 μg/L) concentrations of Li.

conterminous United States↗

Mobile Bay

Mobile Bay is the largest bay found in Alabama’s coastal area (Handley et al., 2007). It was named an Estuary of National Significance in 1995 under the U.S. Environmental Protection Agency’s (EPA) National Estuary Program (NEP), and its Comprehensive Conservation Management Plan was completed in 2002. Mobile Bay is 1,070 km 2 (413 miles 2 ) in area and 51 km (32 miles) long, making it the sixth largest estuary in the continental United States (Mobile Bay NEP, 2008). Its ecosystem provides habitat for more than 300 species of birds, 310 species of fish, 68 species of reptiles, 57 species of mammals, 40 species of amphibians, and 15 species of shrimp (Mobile Bay NEP, 1997). Mobile Bay lies between the Mississippi and Atlantic Flyways (Mobile Bay NEP, 2003). Commercial and residential development and industrial use is heavy in the Mobile Bay area. Although local growth and industrial markets support the Mobile Bay area economy, the resulting environmental damage to the very ecosystem upon which they depend remains a threat to the environment, economy, and population. The Mobile Bay ecosystem boasts high biological diversity and productivity and supports many freshwater and saltwater species of recreational and commercial importance. The great diversity of Mobile Bay reflects the diversity of Alabama, which is home to the largest number of different plant and animal species of all states east of the Mississippi River (Stein, 2002), and is bolstered by the unique climate and geographic conditions surrounding the bay. Freshwater inflow from the Mobile-Tensaw River Delta, ranging from 60,000 to 3,700,000 gallons per second (Wallace, 1996), mixes with saltwater from the Gulf of Mexico, which enters Mobile Bay via wind and tides (Burgan and Engle, 2006). Because of the unique conditions surrounding Mobile Bay, including shallow waters, a dynamic climate, and artificial hydrologic modifications—such as the construction of the Mobile Bay Causeway in the 1920s, which serves as an unintentional barrier between Delta waters north of the Causeway and saline waters south of the Causeway, the salinity of Mobile Bay is highly variable. Mobile Bay receives an average of 165 cm (65 inches) of rain per year from tropical storms, summer thunderstorms, and winter cold fronts (Stout et al., 1998). The climate and geography that have made Mobile Bay so rich in resources have also contributed to the threats surrounding its ecosystem. The extensive amount of rain in Mobile Bay creates large amounts of runoff, polluting the waters with fertilizers, chemicals, sediment, oil, trash, and sewage (Mobile Bay NEP, 1997). Tourism, ecotourism, recreational and commercial fishing, recreational boating, shipping, and chemical, pulp, and paper production are significant industries in Mobile Bay and the surrounding areas. Despite the approximate \$3 billion and 55,000 jobs these industries bring into the community (Alabama Tourism Department, 2010), the growth, development, and environmental stress they create are major threats to the Mobile Bay ecosystem. Among the nation’s states, Alabama ranks fifth in number of different species (144 endemic species), second in number of extinctions that have already occurred (90 extinct species) and fourth in number of species at risk for extinction (14.8% at risk out of 4,533 total species; Stein, 2002). Twenty-one of these threatened and endangered species are found in Mobile Bay, whose brackish waters provide a nursery area for many species of vertebrates and invertebrates. Some of these species include the Alabama sturgeon, Gulf sturgeon, heavy pigtoe mussel, inflated heel-splitter mussel, West Indian manatee, Alabama beach mouse, Perdido beach mouse, Alabama red-bellied turtle, gopher tortoise, Kemp’s ridley sea turtle, green sea turtle, loggerhead sea turtle, eastern indigo snake, flatwoods salamander, piping plover, red-cockaded woodpecker, and wood stork. Habitat loss underlies the decline of some bird species in Mobile Bay, and large mammals such as the red wolf, Florida panther, and Florida black bear are no longer found in the area. However, some rare species, such as the swallow-tailed kite, sandhill crane, and gopher tortoise can still be found (Duke and Kruczynski, 1992). The value of wetlands in Mobile Bay and the rest of the Gulf of Mexico is still being investigated. Although various monetary valuations of wetlands exist, critics remark that undervaluation of wetlands is inevitable (Mobile Bay NEP, 2008) and that estimates often do not place appropriate value on ecological services (Mitsch and Gosselink, 2000). Additionally, many estimates account only for anthropogenic values. One estimate concludes that one acre of wetlands performs \$3,000 worth of water purification each year (Mobile Bay NEP, 1997). With more than 76,890 hectares (190,000 acres) of wetlands in the Mobile Bay area, that equates to a value exceeding one-half billion dollars every year. Tourism, fishing, boating, production, and shipping are significant industries in the Mobile Bay area. More than 90% of fish landed in recreational and commercial fishing in the bay depend on bay habitat, including wetlands, for life requirements (Mobile Bay NEP, 1997). The Port of Mobile is Alabama’s only ocean-ship port (Mobile Bay NEP, 2008). Baldwin County, on the eastern side of the bay, experienced a population increase of 75% from 1990 to 2007, with an 89% increase in housing units (Mobile Bay NEP, 2008). Development and industry support the Mobile Bay economy, but they depend on the continued health, sustainability, and production of the water and living resources of the Mobile Bay ecosystem. Wetland loss, along with other forms of environmental degradation, remains a threat to the Mobile Bay ecosystem and Mobile Bay’s socioeconomic foundation.

Alabama↗

Groundwater quality in Geauga County, Ohio: status, including detection frequency of methane in water wells, 2009, and changes during 1978-2009

Domestic wells that are not safeguarded by regular water-quality testing provide drinking water for 79 percent of the residents of Geauga County, in northeastern Ohio. Since 1978, the U.S. Geological Survey (USGS) has worked cooperatively with the Board of Commissioners and Geauga County Planning Commission to monitor the quality of groundwater in four commonly used aquifers in county&mdash;the glacial deposits, the Pottsville Formation, the Cuyahoga Group, and the Berea Sandstone. A 33-percent growth in population from 1980 to 2009 increased the potential for humans to influence groundwater resources by withdrawing more groundwater, disposing of more human waste near the land surface, treating an expanded network of township roads with deicing salt, and likely using more solvents, pesticides, and other chemicals on the land surface than were used in preceding decades. To describe the status of groundwater quality in 2009 and its suitability for drinking, USGS personnel collected samples of water prior to treatment from 16 wells (mostly domestic) during June 9&ndash;19. The samples were analyzed for 92 properties and constituents, 41 of which had human-health benchmarks to which analytical results could be compared to evaluate suitability for drinking. Four of these benchmarks were exceeded at the following frequencies: arsenic (2 of 16 wells, 12.5 percent), total coliform bacteria (2 of 16 wells, 12.5 percent), fecal coliform bacteria (1 of 14 wells, 7 percent), and sodium (6 of 16 wells, 38 percent). No domestic wells sampled in 2009 exceeded the health-based benchmark of 300 micrograms per liter (&micro;g/L) for manganese, although 5 of 65 wells (8 percent) sampled since 1978 have. Analyses from domestic wells were augmented with water-quality data from seven public-supply well fields that were obtained from the Ohio Environmental Protection Agency. These public-supply data were typically collected between 2000 and 2010 and represent water samples that were collected prior to treatment or that were treated by a method that does not effectively remove the constituents of interest. Similar to the domestic-well data, these data indicated that some samples from public-supply wells have also exceeded health-based benchmarks for arsenic and sodium, along with occasional exceedances of health-based benchmarks for cadmium and lead. Concentrations of nitrate, pesticides, and volatile organic compounds in ground-water samples from domestic and (or) public-supply wells were either considerably less than the human-health benchmarks for these constituents or were not detected. Water-quality data collected in 2009 were also compared to aesthetically based benchmarks developed by the U.S. Environmental Protection Agency, called Secondary Maximum Contaminant Levels (SMCLs). Iron and manganese most frequently exceeded SMCLs (in samples from 10 of 16 domestic wells and in untreated water from 3 of 4 public-supply well fields). To evaluate the frequency of methane detection in water wells in the county, the USGS sampled 16 wells across the county and screened the samples for combustible gas within the headspace (the air above the water in a closed container). Water from three (19 percent) of the wells contained detectable combustible gas (0.10 to 0.40 percent by volume). All three detections were from wells tapping the Cuyahoga Group or the Berea Sandstone, and all detections were less than the lower explosive limit of 5 percent by volume&mdash;the concentration at which methane in air can be flammable if an ignition source is present. Analyses of dissolved gas composition in water from these three wells showed methane concentrations ranging from 0.007 to 1.8 milligrams per liter (mg/L). The primary effect of human activities on groundwater quality found during this study is the input of salinity, or chloride, near land surface. On the basis of ratios of chloride to bromide, the main sources of chloride are road salt and septic leachate rather than oil-field brines (either spilled at land surface or sprayed on roads for dust control). The correlation of chloride concentration to distance of well from road for 31 wells in the county sampled by the U.S. Geological Survey in 1999 suggests that road salt is the dominant source of chloride. The majority of constituents exceeding health-based and aesthetically based benchmarks in groundwater were those that are naturally present in aquifer rocks and sediments rather than constituents introduced by human activities. Concentrations of such natural contaminants are controlled by geochemical processes in the subsurface, particularly by oxidation-reduction (redox) reactions. The categorization of redox conditions based on the water quality of 116 samples collected from 65 wells in Geauga County during 1978 through 2009 indicates that most groundwater samples were strongly reducing (60 percent) or oxic (18 percent). Oxic waters were found only in the Pottsville Formation and Berea Sandstone and were generally associated with nitrate at concentrations of 0.38 to 6.0 mg/L. Strongly reducing waters occurred in all four commonly used aquifers and were associated with the following naturally occurring contaminants: (1) arsenic and manganese at concentrations exceeding the health-based benchmarks (10 &micro;g/L and 300 &micro;g/L, respectively) in some samples, (2) iron and manganese at concentrations exceeding the aesthetically based standards (300 &micro;g/L and 50 &micro;g/L, respectively) in most samples, and (3) total sulfides (consisting of hydrogen sulfide gas with its characteristic rotten-egg odor and [or] iron sulfide minerals that appear as finely disseminated particulates in water). Because of the association of redox conditions with specific contaminants, attempts were made to further document spatially where oxic and strongly reducing conditions occur so that contaminant occurrence can be better anticipated by planners and well owners. Within the Pottsville Formation, wells tapping strongly reducing groundwater tended to have a greater thickness of overlying low-permeability (recharge-inhibiting) material such as clay and shale than other wells tapping oxic or nitrate-reducing groundwater. In the Berea Sandstone, oxic conditions were found at well locations where either depth to groundwater was shallow (less than 45 feet [ft] below land surface) or the measured water level was within the open interval (uncased portion) of the well, whereas strongly reducing groundwater was found at well locations where depths to water were greater than 60 ft below land surface and measured water levels were 15 ft or more above the open interval of the well. To evaluate whether constituent concentrations consistently increased or decreased over time, the strength of the association between sampling year (time) and constituent concentration was statistically evaluated for 116 water-quality samples collected by the USGS in 1978, 1980, 1986, 1999, and 2009 from a total of 65 wells across the county (generally domestic wells or wells serving small businesses or churches). Results indicate that many of the constituents that have been analyzed for decades exhibited no consistent temporal trends at a statistically significant level (p-value less than 0.05); fluctuations in concentrations of these constituents represent natural variation in groundwater quality. Dissolved oxygen, calcium, and sulfate concentrations and chloride:bromide ratios increased over time in one or more aquifers, while pH and concentrations of bromide and dissolved organic carbon decreased over time. Detections of total coliform bacteria and nitrate did not become more frequent from 1986 to 2009, even though potential sources of these constituents, such as number of septic systems (linked to population) and percent developed land in the county, increased during this period.

Geauga County↗

Hormones and pharmaceuticals in groundwater used as a source of drinking water across the United States

This is the first large-scale, systematic assessment of hormone and pharmaceutical occurrence in groundwater used for drinking across the United States. Samples from 1091 sites in Principal Aquifers representing 60% of the volume pumped for drinking-water supply had final data for 21 hormones and 103 pharmaceuticals. At least one compound was detected at 5.9% of 844 sites representing the resource used for public supply across the entirety of 15 Principal Aquifers, and at 11.3% of 247 sites representing the resource used for domestic supply over subareas of nine Principal Aquifers. Of 34 compounds detected, one plastics component (bisphenol A), three pharmaceuticals (carbamazepine, sulfamethoxazole, and meprobamate), and the caffeine degradate 1,7-dimethylxanthine were detected in more than 0.5% of samples. Hydrocortisone had a concentration greater than a human-health benchmark at 1 site. Compounds with high solubility and low K oc were most likely to be detected. Detections were most common in shallow wells with a component of recent recharge, particularly in crystalline-rock and mixed land-use settings. Results indicate vulnerability of groundwater used for drinking water in the U.S. to contamination by these compounds is generally limited, and exposure to these compounds at detected concentrations is unlikely to have adverse effects on human health.

Environmental Science & Technology↗

Sediment characterization and dynamics in Lake Pontchartrain, Louisiana

Lake Pontchartrain in southeastern Louisiana is the largest of several shallow estuaries that together cover over 15,000 km 2 . Wetlands, forests, and large urban areas surround the lake. Primary transport mechanisms of sediments to Lake Pontchartrain include urban runoff, major diversions of the Mississippi River, discharge from streams along the north and west shores, and tidal circulation. Sediments deposited in Lake Pontchartrain are subjected to resuspension and mixing by natural and human activities. Bioturbation and water turbulence throughout the lake are the major mixing agents, and mechanical shell dredging has reworked much of the lake bottom over the last century. Sediment characterization through direct sampling and geophysical surveys indicates that these processes continually rework the top meter of sediment. The lake receives discharge from roadways and industrial and agricultural sources. Contaminants from these sources accumulate in the lake sediments and are an important contributor to the degradation of the estuary. Decline in populations of various benthic organisms, such as shrimp and clams, has been documented in the lake. To characterize the health of this important estuary, the U.S. Geological Survey (USGS) conducted a comprehensive evaluation of the geology, geomorphology, coastal processes, and environmental condition of the Pontchartrain Basin from 1994 to 1997. This report presents an assessment of sediment distribution and quality using a multidisciplinary approach to characterize the influence of various physical and chemical parameters: nearsurface stratigraphy, major trace metal concentrations (Cu, Pb, Zn, and Ni), and short-lived radionuclides ( 210 Pb, 7 Be, and 137 Cs). The results are compared with water-circulation patterns to determine high-resolution sedimentation patterns in the lake. The data show a significant increase in trace metals in the top 1 m of lake sediments. Above this horizon, pollen analysis indicates a correlation with land clearing in the area, a proxy for increasing human development of the surrounding landscape and an increase in surface run-off. The data also show that the top meter of sediment undergoes frequent resuspension during high-energy circulation events and via circulation gyres in the lake. This regular turnover does not allow stratification of recently deposited sediments, restricting the sequestration of contaminated material that enters the lake.

Louisiana↗

Water-quality trend analysis and sampling design for streams in the Red River of the North Basin, Minnesota, North Dakota, and South Dakota, 1970-2001

The Bureau of Reclamation is considering several alternatives to meet the future municipal, rural, and industrial water-supply needs in the Red River of the North (Red River) Basin, and an environmental impact statement is being prepared to evaluate the potential effects of the various alternatives on the water quality and aquatic health in the basin in relation to the historical variability of streamflow and constituent concentration. Therefore, a water-quality trend analysis was needed to determine the amount of natural water-quality variability that can be expected to occur in the basin, to determine if significant water-quality changes have occurred as a result of human activities, to explore potential causal mechanisms for water-quality changes, and to establish a baseline from which to monitor future water-quality trends. This report presents the results of a study conducted by the U.S. Geological Survey, in cooperation with the Bureau of Reclamation, to analyze historical water-quality trends in two dissolved major ions, dissolved solids, three nutrients, and two dissolved trace metals for nine streamflow-gaging stations in the basin. Annual variability in streamflow in the Red River Basin was high during the trend-analysis period (1970-2001). The annual variability affects constituent concentrations in individual tributaries to the Red River and, in turn, affects constituent concentrations in the main stem of the Red River because of the relative streamflow contribution from the tributaries to the main stem. Therefore, an annual concentration anomaly, which is an estimate of the interannual variability in concentration that can be attributed to long-term variability in streamflow, was used to analyze annual streamflow-related variability in constituent concentrations. The concentration trend is an estimate of the long-term systematic changes in concentration that are unrelated to seasonal or long-term variability in streamflow. Concentrations that have both the seasonal and annual variability removed are called standardized concentrations. Numerous changes that could not be attributed to natural streamflow-related variability occurred in the standardized concentrations during the trend-analysis period. During various times from the late 1970's to the mid-1990's, significant increases occurred in standardized dissolved sulfate, dissolved chloride, and dissolved- solids concentrations for eight of the nine stations for which water-quality trends were analyzed. Significant increases also occurred from the early 1980's to the mid-1990's for standardized dissolved nitrite plus nitrate concentrations for the main-stem stations. The increasing concentrations for the main-stem stations indicate the upward trends may have been caused by human activities along the main stem of the Red River. Significant trends for standardized total ammonia plus organic nitrogen concentrations occurred for most stations. The fitted trends for standardized total phosphorus concentrations for one tributary station increased from the late 1970's to the early 1980's and decreased from the early 1980's to the mid-1990's. Small but insignificant increases occurred for two main-stem stations. No trends were detected for standardized dissolved iron or dissolved manganese concentrations. However, the combination of extreme high-frequency variability, few data, and the number of censored values may have disguised the streamflow-related variability for iron. The time-series model used to detect historical concentration trends also was used to evaluate sampling designs to monitor future water-quality trends. Various sampling designs were evaluated with regard to their sensitivity to detect both annual and seasonal trends during three 4-month seasons. A reasonable overall design for detecting trends for all stations and constituents consisted of eight samples per year, with monthly sampling from April to August and bimonthly sampling from October to February.

Scientific Investigations Report↗

A novel picorna-like virus in a Wabash Pigtoe (Fusconaia flava) from the upper Mississippi River, USA

Unionid mussels are threatened by multiple environmental stressors and have experienced mass mortality events over the last several decades, but the role of infectious disease in unionid health and population declines remains poorly understood. Although several microbial agents have been found in unionids, to date only one virus has been documented—Lea plague virus ( Arenaviridae ) in propagated Triangle Shell mussels ( Hyriopsis cumingii ) in China. We used next-generation DNA sequencing to screen hemolymph of seven individuals of five unionid species from the Upper Mississippi River basin, USA for viruses. We identified the complete polyprotein gene of a novel picornalike virus in one individual of the Wabash Pigtoe ( Fusconaia flava ). The virus is a member of the Nora virus clade of picornalike viruses and is most closely related to viruses from arthropods in China. We did not detect viruses in another Wabash Pigtoe or in animals of the other four species. It is premature to make inferences about the role of this virus in the health of Wabash Pigtoes or other unionid species or the origin or transmission of this virus. Nevertheless, to our knowledge, our results represent the first report of a virus in wild North American unionids. Technologies based on next-generation DNA sequencing should prove useful for identifying new viruses and investigating their role in unionid health and disease.

Minnesota, Wisconsin↗

Intercomparison of same-day remote sensing data for measuring winter cover crop biophysical traits

Winter cover crops are planted during the fall to reduce nitrogen losses and soil erosion and improve soil health. Accurate estimations of winter cover crop performance and biophysical traits including biomass and fractional vegetative groundcover support accurate assessment of environmental benefits. We examined the comparability of measurements between ground-based and spaceborne sensors as well as between processing levels (e.g., surface vs. top-of-atmosphere reflectance) in estimating cover crop biophysical traits. This research examined the relationships between SPOT 5, Landsat 7, and WorldView-2 same-day paired satellite imagery and handheld multispectral proximal sensors on two days during the 2012–2013 winter cover crop season. We compared two processing levels from three satellites with spatially aggregated proximal data for red and green spectral bands as well as the normalized difference vegetation index (NDVI). We then compared NDVI estimated fractional green cover to in-situ photographs, and we derived cover crop biomass estimates from NDVI using existing calibration equations. We used slope and intercept contrasts to test whether estimates of biomass and fractional green cover differed statistically between sensors and processing levels. Compared to top-of-atmosphere imagery, surface reflectance imagery were more closely correlated with proximal sensors, with intercepts closer to zero, regression slopes nearer to the 1:1 line, and less variance between measured values. Additionally, surface reflectance NDVI derived from satellites showed strong agreement with passive handheld multispectral proximal sensor-sensor estimated fractional green cover and biomass (adj. R 2 = 0.96 and 0.95; RMSE = 4.76% and 259 kg ha−1, respectively). Although active handheld multispectral proximal sensor-sensor derived fractional green cover and biomass estimates showed high accuracies (R 2 = 0.96 and 0.96, respectively), they also demonstrated large intercept offsets (−25.5 and 4.51, respectively). Our results suggest that many passive multispectral remote sensing platforms may be used interchangeably to assess cover crop biophysical traits whereas SPOT 5 required an adjustment in NDVI intercept. Active sensors may require separate calibrations or intercept correction prior to combination with passive sensor data. Although surface reflectance products were highly correlated with proximal sensors, the standardized cloud mask failed to completely capture cloud shadows in Landsat 7, which dampened the signal of NIR and red bands in shadowed pixels.

Maryland↗

Microphotographs of cyanobacteria documenting the effects of various cell-lysis techniques

Cyanotoxins are a group of organic compounds biosynthesized intracellularly by many species of cyanobacteria found in surface water. The United States Environmental Protection Agency has listed cyanotoxins on the Safe Drinking Water Act's Contaminant Candidate List 3 for consideration for future regulation to protect public health. Cyanotoxins also pose a risk to humans and other organisms in a variety of other exposure scenarios. Accurate and precise analytical measurements of cyanotoxins are critical to the evaluation of concentrations in surface water to address the human health and ecosystem effects. A common approach to total cyanotoxin measurement involves cell membrane disruption to release the cyanotoxins to the dissolved phase followed by filtration to remove cellular debris. Several methods have been used historically, however no standard protocols exist to ensure this process is consistent between laboratories before the dissolved phase is measured by an analytical technique for cyanotoxin identification and quantitation. No systematic evaluation has been conducted comparing the multiple laboratory sample processing techniques for physical disruption of cell membrane or cyanotoxins recovery. Surface water samples collected from lakes, reservoirs, and rivers containing mixed assemblages of organisms dominated by cyanobacteria, as well as laboratory cultures of species-specific cyanobacteria, were used as part of this study evaluating multiple laboratory cell-lysis techniques in partnership with the U.S. Environmental Protection Agency. Evaluated extraction techniques included boiling, autoclaving, sonication, chemical treatment, and freeze-thaw. Both treated and untreated samples were evaluated for cell membrane integrity microscopically via light, epifluorescence, and epifluorescence in the presence of a DNA stain. The DNA stain, which does not permeate live cells with intact membrane structures, was used as an indicator for cyanotoxin release into the dissolved phase. Of the five techniques, sonication (at 70 percent) was most effective at complete cell destruction while QuikLyse (Trademarked) was least effective. Autoclaving, boiling, and sequential freeze-thaw were moderately effective in physical destruction of colonies and filaments.

Open-File Report↗

Effects of wastewater effluent discharge and treatment facility upgrades on environmental and biological conditions of Indian Creek, Johnson County, Kansas, June 2004 through June 2013

Indian Creek is one of the most urban drainage basins in Johnson County, Kansas, and environmental and biological conditions of the creek are affected by contaminants from point and other urban sources. The Johnson County Douglas L. Smith Middle Basin (hereafter referred to as the “Middle Basin”) and Tomahawk Creek Wastewater Treatment Facilities (WWTFs) discharge to Indian Creek. In summer 2010, upgrades were completed to increase capacity and include biological nutrient removal at the Middle Basin facility. There have been no recent infrastructure changes at the Tomahawk Creek facility; however, during 2009, chemically enhanced primary treatment was added to the treatment process for better process settling before disinfection and discharge with the added effect of enhanced phosphorus removal. The U.S. Geological Survey, in cooperation with Johnson County Wastewater, assessed the effects of wastewater effluent on environmental and biological conditions of Indian Creek by comparing two upstream sites to four sites located downstream from the WWTFs using data collected during June 2004 through June 2013. Environmental conditions were evaluated using previously and newly collected discrete and continuous data and were compared with an assessment of biological community composition and ecosystem function along the upstream-downstream gradient. This study improves the understanding of the effects of wastewater effluent on stream-water and streambed sediment quality, biological community composition, and ecosystem function in urban areas. After the addition of biological nutrient removal to the Middle Basin WWTF in 2010, annual mean total nitrogen concentrations in effluent decreased by 46 percent, but still exceeded the National Pollutant Discharge Elimination System (NPDES) wastewater effluent permit concentration goal of 8.0 milligrams per liter (mg/L); however, the NPDES wastewater effluent permit total phosphorus concentration goal of 1.5 mg/L or less was achieved at the Middle Basin WWTF. At the Tomahawk Creek WWTF, after the addition of chemically enhanced primary treatment in 2009, effluent discharges also had total phosphorus concentrations below 1.5 mg/L. After the addition of biological nutrient removal, annual total nitrogen and phosphorus loads from the Middle Basin WWTF decreased by 42 and 54 percent, respectively, even though effluent volume increased by 11 percent. Annual total phosphorus loads from the Tomahawk Creek WWTF after the addition of chemically enhanced primary treatment decreased by 54 percent despite a 33-percent increase in effluent volume. Total nitrogen and phosphorus from the WWTFs contributed between 30 and nearly 100 percent to annual nutrient loads in Indian Creek depending on streamflow conditions. In-stream total nitrogen primarily came from wastewater effluent except during years with the highest streamflows. Most of the in-stream total phosphorus typically came from effluent during dry years and from other urban sources during wet years. During 2010 through 2013, annual mean discharge from the Middle Basin WWTF was about 75 percent of permitted design capacity. Annual nutrient loads likely will increase when the facility is operated at permitted design capacity; however, estimated maximum annual nutrient loads from the Middle Basin WWTF were 27 to 38 percent lower than before capacity upgrades and the addition of biological nutrient removal to treatment processes. Thus, the addition of biological nutrient removal to the Middle Basin wastewater treatment process should reduce overall nutrient loads from the facility even when the facility is operated at permitted design capacity. The effects of wastewater effluent on the water quality of Indian Creek were most evident during below-normal and normal streamflows (about 75 percent of the time) when wastewater effluent represented about 24 percent or more of total streamflow. Wastewater effluent had the most substantial effect on nutrient concentrations in Indian Creek. Total and inorganic nutrient concentrations at the downstream sites during below-normal and normal streamflows were 10 to 100 times higher than at the upstream sites, even after changes in treatment practices at the WWTFs. Median total phosphorus concentrations during below-normal and normal streamflows at a downstream site were 43 percent lower following improvements in wastewater treatment processes. Similar decreases in total nitrogen were not observed, likely because total nitrogen concentrations only decreased in Middle Basin effluent and wastewater contributed a higher percentage to streamflows when nutrient samples were collected during the after-upgrade period. The wastewater effluent discharges to Indian Creek caused changes in stream-water quality that may affect biological community structure and ecosystem processes, including higher concentrations of bioavailable nutrients (nitrate and orthophosphorus) and warmer water temperatures during winter months. Other urban sources of contaminants also caused changes in stream-water quality that may affect biological community structure and ecosystem processes, including higher turbidities downstream from construction areas and higher specific conductance and chloride concentrations during winter months. Chloride concentrations exceeded acute and chronic exposure criteria at all Indian Creek study sites, regardless of wastewater influence, for weeks or months during winter. Streambed sediment chemistry was affected by wastewater (elevated nutrient and organic wastewater-indicator compound concentrations) and other contaminants from urban sources (elevated polyaromatic hydrocarbon concentrations). Overall habitat conditions were suboptimal or marginal at all sites; general decline in habitat conditions along the upstream-downstream gradient likely was caused by the cumulative effects of urbanization with increasing drainage basin size. Wastewater effluent likely affected algal periphyton biomass and community composition, primary production, and community respiration in Indian Creek. Functional stream health, evaluated using a preliminary framework based on primary production and community respiration, was mildly or severely impaired at most downstream sites relative to an urban upstream Indian Creek site. The mechanistic cause of the changes in these biological variables are unclear, though elevated nutrient concentrations were positively correlated with algal biomass, primary production, and community respiration. Macroinvertebrate communities indicated impairment at all sites, and Kansas Department of Health and Environment aquatic life support scores indicated conditions nonsupporting of aquatic life, regardless of wastewater influences. Urban influences, other than wastewater effluent discharge, likely control macroinvertebrate community structure in Indian Creek. Changes in treatment processes at the Middle Basin and Tomahawk Creek WWTFs improved wastewater effluent quality and decreased nutrient loads, but wastewater effluent discharges still had negative effects on the environmental and biological conditions at downstream Indian Creek sites. Wastewater effluent discharge into Indian Creek likely contributed to changes in measures of ecosystem structure (streamflow, water and streambed-sediment chemistry, algal biomass, and algal periphyton community composition) and function (primary production and community respiration) along the upstream-downstream gradient. Wastewater effluent discharges maintained streamflows and increased nutrient concentrations, algal biomass, primary production, and community respiration at the downstream sites. Functional stream health was severely impaired downstream from the Middle Basin WWTF and mildly impaired downstream from the Tomahawk WWTF relative to the urban upstream site. As distance from the Middle Basin WWTF increased, nutrient concentrations, algal biomass, primary production, and community respiration decreased, and functional stream health was no longer impaired 9.5 kilometers downstream from the discharge relative to the urban upstream site. Therefore, although wastewater effluent caused persistent changes in environmental and biological conditions and functional stream health at sites located immediately downstream from WWTF effluent discharges, some recovery to conditions more similar to the urban upstream site occurred within a relatively short distance.

Kansas↗

Threats and stressors to the health of the ecosystems of Lakes Mead and Mohave: Chapter 6 in A synthesis of aquatic science for management of Lakes Mead and Mohave

Ecosystem impacts from visitor activities or natural environmental change are important concerns in all units of the National Park system. Possible impacts to aquatic ecosystems at Lake Mead National Recreation Area (LMNRA) are of particular concern because of the designation of Lakes Mead and Mohave as critical habitat for the federally listed endangered razorback sucker (Xyrauchen texanus), the significance of the sport fishery, and the regional importance of its habitats to more than 90 documented species of waterbirds. Potential threats to shoreline habitats are of concern not only for their ecosystem values but also for maintaining the recreational setting. Many areas adjacent to the shorelines of Lakes Mead and Mohave are designated wilderness areas. For purposes of this document, stressors are any chemical, biological, or physical agent that has a detrimental effect on aquatic ecosystems at the organism, population, or community level. Human-made stressors at Lakes Mead and Mohave include direct effects of recreation on the lakes, like boating and fishing, as well as indirect effects of activities away from the lakes, such as growing population and increasing urbanization. Common natural environmental stressors include extended changes in climate (precipitation or temperature), or the erosion, transport, and loading of chemical constituents in rocks and sediments to aquatic environments. Human activity also can exacerbate natural stressors in a variety of ways.

Circular↗