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Rethinking foundation species in a changing world: The case for Rhododendron maximum as an emerging foundation species in shifting ecosystems of the southern Appalachians

“Foundation species” are widespread, abundant species that play critical roles in structuring ecosystem characteristics and processes. Ecosystem change in response to human activities, climate change, disease introduction, or other environmental conditions may promote the emergence of new foundation species or the decline of previously important foundation species. We present rhododendron ( Rhododendron maximum ) as an example of an emerging foundation species in riparian forest and headwater stream ecosystems of the southern Appalachian Mountains and use its example to propose a dynamic approach to recognizing foundation species. As other species have declined, rhododendron has increased in abundance, biomass, and ecosystem importance, and now dominates the riparian zones and mesic uplands of much of the region. Rhododendron structures, stabilizes, and modulates functions within both terrestrial and aquatic ecosystems. Studies of forest ecosystem response to environmental conditions indicate that rhododendron may increase the resistance and resilience of its associated ecosystems to predicted anthropogenic stress, including climate change, nitrogen enrichment, and invasive species. A more dynamic conception of foundation species as dependent on ecosystem states will help ecologists to focus on ecosystem processes and services, rather than on historically dominant species, for restoration strategies.

Alabama, Georgia, North Carolina, South Carolina, ↗

Invertebrate prey contributions to juvenile Coho Salmon diet from riparian habitats along three Alaska streams: Implications for environmental change

Stream fish rely on a mix of terrestrial and aquatic prey sources. While the importance of terrestrial invertebrates as a food source for stream fish is well documented, the role of aquatic insects that emerge from the stream as winged adult insects (aquatic winged adults) and return to the stream as prey is less understood. In this study we determined the proportion of total diet for stream-rearing juvenile Coho Salmon ( Oncorhynchus kisutch) that is derived from terrestrial and aquatic winged adult invertebrates which enter the stream from riparian habitats and consider how those cross-ecosystem prey contributions vary based on riparian habitat type. Study reaches were identified in three streams within the Kenai River watershed of Alaska that were representative of habitats found throughout the region and riparian vegetation was classified into grass/sedge, shrub and tree types using LiDAR. Juvenile Coho Salmon stomach contents were sampled seasonally in study reaches over a two-year period and ingested invertebrates were identified by taxa, life stage and origin. Our results showed that aquatic winged adult prey contributions to juvenile salmon diet were significantly lower in the grass/sedge study reach, and cross-ecosystem invertebrate prey represented a significantly higher proportion of juvenile salmon diet in the tree study reach. Invertebrate prey in the grass/sedge reach were composed primarily of the larval life stage of aquatic winged adults. These results suggest that change in riparian vegetation from tree/shrub to grass/sedge along Kenai streams as projected by regional climate change models, or that results from anthropogenic modification, will likely lead to lower availability of cross-ecosystem prey for stream fish. Management of riparian buffers along streams to preserve or increase occurrence of trees and shrubs is likely to help mitigate impacts of those possible changes.

Alaska↗

Geomorphic and vegetation processes of the Willamette River floodplain, Oregon: current understanding and unanswered science questions

This report summarizes the current understanding of floodplain processes and landforms for the Willamette River and its major tributaries. The area of focus encompasses the main stem Willamette River above Newberg and the portions of the Coast Fork Willamette, Middle Fork Willamette, McKenzie, and North, South and main stem Santiam Rivers downstream of U.S. Army Corps of Engineers dams. These reaches constitute a large portion of the alluvial, salmon-bearing rivers in the Willamette Basin. The geomorphic, or historical, floodplain of these rivers has two zones - the active channel where coarse sediment is mobilized and transported during annual flooding and overbank areas where fine sediment is deposited during higher magnitude floods. Historically, characteristics of the rivers and geomorphic floodplain (including longitudinal patterns in channel complexity and the abundance of side channels, islands and gravel bars) were controlled by the interactions between floods and the transport of coarse sediment and large wood. Local channel responses to these interactions were then shaped by geologic features like bedrock outcrops and variations in channel slope. Over the last 150 years, floods and the transport of coarse sediment and large wood have been substantially reduced in the basin. With dam regulation, nearly all peak flows are now confined to the main channels. Large floods (greater than 10-year recurrence interval prior to basinwide flow regulation) have been largely eliminated. Also, the magnitude and frequency of small floods (events that formerly recurred every 2–10 years) have decreased substantially. The large dams trap an estimated 50–60 percent of bed-material sediment—the building block of active channel habitats—that historically entered the Willamette River. They also trap more than 80 percent of the estimated bed material in the lower South Santiam River and Middle and Coast Forks of the Willamette River. Downstream, revetments further decrease bed-material supply by an unknown amount because they limit bank erosion and entrainment of stored sediment. The rivers, geomorphic floodplain, and vegetation within the study area have changed noticeably in response to the alterations in floods and coarse sediment and wood transport. Widespread decreases have occurred in the rates of meander migration and avulsions and the number and diversity of landforms such as gravel bars, islands, and side channels. Dynamic and, in some cases, multi-thread river segments have become stable, single-thread channels. Preliminary observations suggest that forest area has increased within the active channel, further reducing the area of unvegetated gravel bars. Alterations to floods and sediment transport and ongoing channel, floodplain, and vegetation responses result in a modern Willamette River Basin. Here, the floodplain influenced by the modern flow and sediment regimes, or the functional floodplain, is narrower and inset with the broader and older geomorphic floodplain. The functional floodplain is flanked by higher elevation relict floodplain features that are no longer inundated by modern floods. The corridor of present- day active channel surfaces is narrower, enabling riparian vegetation to establish on formerly active gravel bar surfaces. The modern Willamette River Basin with its fundamental changes in the flood, sediment transport, and large wood regimes has implications for future habitat conditions. System-wide future trends probably include narrower floodplains and a lower diversity of landforms and habitats along the Willamette River and its major tributaries compared to historical patterns and today. Furthermore, specific conditions and future trends will probably vary between geologically stable, anthropogenically stable, and dynamic reaches. The middle and lower segments of the Willamette River are geologically stable, whereas the South Santiam and Middle Fork Willamette Rivers were historically dynamic, but are now largely stable in response to flow regulation and revetment construction. The upper Willamette and North Santiam Rivers retain some dynamic characteristics, and provide the greatest diversity of aquatic and riparian habitats under the current flow and sediment regime. The McKenzie River has some areas that are more dynamic, whereas other sections are stable due to geology or revetments. Historical reductions in channel dynamism also have implications for ongoing and future recruitment and succession of floodplain forests. For instance, the succession of native plants like black cottonwood is currently limited by (1) fewer low-elevation gravel bars for stand initiation; (2) altered streamflow during seed release, germination, and stand initiation; (3) competition from introduced plant species; and (4) frequent erosion of young vegetation in some locations because scouring flows are concentrated within a narrow channel corridor. Despite past alterations, the Willamette River Basin has many of the physical and ecological building blocks necessary for highly functioning rivers. Management strategies, including environmental flow programs, river and floodplain restoration, revetment modifications, and reclamation of gravel mines, are underway to mitigate some historical changes. However, there are some substantial gaps in the scientific understanding of the modern Willamette basin that is needed to efficiently integrate these blocks and to establish realistic objectives for future conditions. Unanswered questions include: 1. What is the distribution and diversity of landforms and habitats along the Willamette River and its tributaries? 2. What is the extent of today’s functional floodplain—the part of the river corridor actively formed and modified by fluvial processes? 3. How are landforms and habitats in the Willamette River Basin created and sustained by present-day flow and sediment conditions? 4. How is the succession of native floodplain vegetation shaped by present-day flow and sediment conditions? Answering these questions will produce baseline data on the current distributions of landforms and habitats (question 1), the extent of the functional floodplain (question 2), and the effects of modern flow and sediment regimes on future floodplain landforms, habitats, and vegetation succession (questions 3 and 4). Addressing questions 1 and 2 is a logical next step because they underlie questions 3 and 4. Addressing these four questions would better characterize the modern Willamette Basin and help in implementing and setting realistic targets for ongoing management strategies, demonstrating their effectiveness at the site and basin scales, and anticipating future trends and conditions.

Oregon↗

Use of tolerance values to diagnose water-quality stressors to aquatic biota in New England streams

Identification of stressors related to biological impairment is critical to biological assessments. We applied nationally derived tolerance indicator values for four water-quality variables to fish and benthic macroinvertebrate assemblages at 29 sites along an urban gradient in New England. Tolerance indicator values (TIVs), as biologically based predictors of water-quality variables, were determined for dissolved oxygen, nitrite plus nitrate (nitrate), total phosphorus, and water temperature for each site based on observed biological assemblages (TIVO), and for expected assemblages (TIVE). The quotient method, based on a ratio of the TIVs for observed and expected assemblages (tolerance units), was used to diagnose potential water-quality stressors. In addition, the ratio of measured water-quality values to water-quality criteria (water-quality units) was calculated for each water-quality variable to assess measured water-quality stressors. Results from a RIVPACS predictive model for benthic macroinvertebrates and Bray-Curtis dissimilarity for fish were used to classify sites into categories of good or impaired ecological condition. Significant differences were detected between good and impaired sites for all biological tolerance units (fish and benthic macroinvertebrate assemblages averaged) except for nitrate (P = 0.480), and for all water-quality units except for nitrate (P = 0.183). Diagnosis of water-quality stressors at selected sites was, in general, consistent with State-reported causes of impairment. Tolerance units for benthic macroinvertebrate and fish assemblages were significantly correlated for water temperature (P = 0.001, r = 0.63), dissolved oxygen (P = 0.001, r = 0.61), and total phosphorus (P = 0.001, r = 0.61), but not for nitrate (P = 0.059, r = -0.35). Differences between the two assemblages in site-specific diagnosis of water-quality stressors may be the result of differences in nitrate tolerance.

Ecological Indicators↗

Spatial and temporal patterns in Arctic mosquito abundance

Organisms that undergo a shift in ontogeny and habitat type often change their spatial distribution throughout their life cycle, but how this affects population dynamics remains poorly understood. We examined spatial and temporal patterns in Aedes nigripes abundance, a widespread univoltine Arctic mosquito species (Diptera: Culicidae), hypothesizing that the spatial distribution of adults would be closely tied to aquatic habitat. We tracked adult densities of A. nigripes near Kangerlussuaq, Greenland using emergence traps, CO 2 -baited traps, and sweep-nets. In back-to-back years of sampling (2017 and 2018) we found two-fold variation in overall abundance. Adults were spatially patchy when first emerging from aquatic habitats but within a week, mean capture rates for host-seeking adult females were similar across locations, even in places far from larval habitat. Daily variation in mosquito captures was primarily explained by weather, with virtually no mosquito activity when temperatures averaged less than 8°C or wind speeds exceeded 6 m/s. Gravid females (3% of resting adults) were spatially patchy on the landscape, but not always in the same places where most adults emerged. The spatial distribution of adults is quickly uncoupled from the spatial distribution of larvae because A. nigripes females may disperse far from their natal habitats in search of a blood-meal and high-quality oviposition habitat. 8. This research highlights the value of studying ecological processes that act at disparate life stages for understanding the population biology of organisms with complex life cycles.

Ecological Applications↗

Multi-decadal trophic shifts in Lake Erie yellow perch Perca flavescens

In Lake Erie, yellow perch Perca flavescens support vast commercial and recreational fisheries, yet populations have recently declined. Using N = 5889 yellow perch stomachs collected from 1997 to 2021, we explored trends in the feeding ecology and trophic level of yellow perch with generalized additive models. Models revealed a significant decrease in yellow perch trophic level (−0.15 trophic levels in the last decade), and significant dietary shifts. Yellow perch have shifted away from feeding on piscine prey and round goby Neogobius melanostomus over the 25-year period, and now feed on invertebrates more frequently—including invasive waterfleas ( Bythotrephes longimanus and Cercopagis pengoi ) and chironomids. Dietary patterns appear to reflect broad ecological changes—invasive waterfleas have proliferated while populations of forage fish and round goby have declined. Furthermore, hypoxia events have increased in duration and severity, which may explain observed increases in chironomid consumption, which are hypoxia tolerant. This study demonstrates trophic adaptability in yellow perch, which have changed feeding behavior and trophic position in response to novel invaders and changing environmental conditions.

Lake Erie↗

Prospective HyspIRI global observations of tidal wetlands

Tidal wetlands are highly productive and act as critical habitat for a wide variety of plants, fish, shellfish, and other wildlife. These ecotones between aquatic and terrestrial environments also provide protection from storm damage, run-off filtering, and recharge of aquifers. Many wetlands along coasts have been exposed to stress-inducing alterations globally, including dredge and fill operations, hydrologic modifications, pollutants, impoundments, fragmentation by roads/ditches, and sea level rise. For wetland protection and sensible coastal development, there is a need to monitor these ecosystems at global and regional scales. Recent advances in satellite sensor design and data analysis are providing practical methods for monitoring natural and man-made changes in wetlands. However, available satellite remote sensors have been limited to mapping primarily wetland location and extent. This paper describes how the HyspIRI hyperspectral and thermal infrared sensors can be used to study and map key ecological properties, such as species composition, biomass, hydrology, and evapotranspiration of tidal salt and brackish marshes and mangroves, and perhaps other major wetland types, including freshwater marshes and wooded/shrub wetlands.

Remote Sensing of Environment↗

Influence of sediment chemistry and sediment toxicity on macroinvertebrate communities across 99 wadable streams of the Midwestern USA

Simultaneous assessment of sediment chemistry, sediment toxicity, and macroinvertebrate communities can provide multiple lines of evidence when investigating relations between sediment contaminants and ecological degradation. These three measures were evaluated at 99 wadable stream sites across 11 states in the Midwestern United States during the summer of 2013 to assess sediment pollution across a large agricultural landscape. This evaluation considers an extensive suite of sediment chemistry totaling 274 analytes (polycyclic aromatic hydrocarbons, organochlorine compounds, polychlorinated biphenyls, polybrominated diphenyl ethers, trace elements, and current-use pesticides) and a mixture assessment based on the ratios of detected compounds to available effects-based benchmarks. The sediments were tested for toxicity with the amphipod Hyalella azteca (28-d exposure), the midge Chironomus dilutus (10-d), and, at a few sites, with the freshwater mussel Lampsilis siliquoidea (28-d). Sediment concentrations, normalized to organic carbon content, infrequently exceeded benchmarks for aquatic health, which was generally consistent with low rates of observed toxicity. However, the benchmark-based mixture score and the pyrethroid insecticide bifenthrin were significantly related to observed sediment toxicity. The sediment mixture score and bifenthrin were also significant predictors of the upper limits of several univariate measures of the macroinvertebrate community (EPT percent, MMI (Macroinvertebrate Multimetric Index) Score, Ephemeroptera and Trichoptera richness) using quantile regression. Multivariate pattern matching (Mantel-like tests) of macroinvertebrate species per site to identified contaminant metrics and sediment toxicity also indicate that the sediment mixture score and bifenthrin have weak, albeit significant, influence on the observed invertebrate community composition. Together, these three lines of evidence (toxicity tests, univariate metrics, and multivariate community analysis) suggest that elevated contaminant concentrations in sediments, in particular bifenthrin, is limiting macroinvertebrate communities in several of these Midwest streams.

Science of the Total Environment↗

Design of Cycle 3 of the National Water-Quality Assessment Program, 2013-2022: Part 1: Framework of Water-Quality Issues and Potential Approaches

In 1991, the U.S. Congress established the U.S. Geological Survey (USGS) National Water-Quality Assessment (NAWQA) Program to develop long-term, nationally consistent information on the quality of the Nation's streams and groundwater. Congress recognized the critical need for this information to support scientifically sound management, regulatory, and policy decisions concerning the increasingly stressed water resources of the Nation. The long-term goals of NAWQA are to: (1) assess the status of water-quality conditions in the United States, (2) evaluate long-term trends in water-quality conditions, and (3) link status and trends with an understanding of the natural and human factors that affect water quality. These goals are national in scale, include both surface water and groundwater, and include consideration of water quality in relation to both human uses and aquatic ecosystems. Since 1991, NAWQA assessments and findings have fostered and supported major improvements in the availability and use of unbiased scientific information for decisionmaking, resource management, and planning at all levels of government. These improvements have enabled agencies and stakeholders to cost-effectively address a wide range of water-quality issues related to natural and human influences on the quality of water and potential effects on aquatic ecosystems and human health (http://water.usgs.gov/nawqa/xrel.pdf). NAWQA, like all USGS programs, provides policy relevant information that serves as a scientific basis for decisionmaking related to resource management, protection, and restoration. The information is freely available to all levels of government, nongovernmental organizations, industry, academia, and the public, and is readily accessible on the NAWQA Web site and other diverse formats to serve the needs of the water-resource community at different technical levels. Water-quality conditions in streams and groundwater are described in more than 1,700 publications (available online at http://water.usgs.gov/nawqa/bib/), and are documented by more than 14 million data records representing about 7,600 stream sites, 8,100 wells, and 2,000 water-quality and ecological constituents that are available from the NAWQA data warehouse (http://infotrek.er.usgs.gov/traverse/f?p=NAWQA:HOME:0). The Program promotes collaboration and liaison with government officials, resource managers, industry representatives, and other stakeholders to increase the utility and relevance of NAWQA science to decisionmakers. As part of this effort, NAWQA supports integration of data from other organizations into NAWQA assessments, where appropriate and cost-effective, so that more comprehensive findings are available across geographic and temporal scales.

Open-File Report↗

A quantitative assessment of the conservation benefits of the Wetlands Reserve Program to amphibians

The Mississippi Alluvial Valley (MAV) originally consisted of nearly contiguous bottomland hardwood (BLH) forest encompassing approximately 10 million hectares. Currently, only 20–25% of the historical BLH forests remain in small patches fragmented by agricultural lands. The Wetlands Reserve Program (WRP) was established to restore and protect the functions and values of wetlands in agricultural landscapes. To assess the potential benefit of WRP restoration to amphibians, we surveyed 30 randomly selected WRP sites and 20 nearby agricultural sites in the Mississippi Delta. We made repeat visits to each site from May to August 2008 and performed both visual encounter and vocalization surveys. We analyzed the encounter history data for 11 anuran species using a Bayesian hierarchical occupancy model that estimated detection probability and probability of occurrence simultaneously for each species. Nine of the 11 species had higher probabilities of occurrence at WRP sites compared to agriculture. Derived estimates of species richness were also higher for WRP sites. Five anuran species were significantly more likely to occur in WRP than in agriculture, four of which were among the most aquatic species. It appears that the restoration of a more permanent hydrology at the WRP sites may be the primary reason for this result. Although amphibians represent only one group of wildlife species, they are useful for evaluating restoration benefits for wildlife because of their intermediate trophic position. The methods used in this study to evaluate the benefit of restoration could be used in other locations and with other groups of indicator species.

Mississippi↗

Imported Asian swamp eels (Synbranchidae: Monopterus) in North American live food markets: Potential vectors of non-native parasites

Since the 1990s, possibly earlier, large numbers of Asian swamp eels (Synbranchidae: Monopterus spp.), some wild-caught, have been imported live from various countries in Asia and sold in ethnic food markets in cities throughout the USA and parts of Canada. Such markets are the likely introduction pathway of some, perhaps most, of the five known wild populations of Asian swamp eels present in the continental United States. This paper presents results of a pilot study intended to gather baseline data on the occurrence and abundance of internal macroparasites infecting swamp eels imported from Asia to North American retail food markets. These data are important in assessing the potential role that imported swamp eels may play as possible vectors of non-native parasites. Examination of the gastrointestinal tracts and associated tissues of 19 adult-sized swamp eels—identified as M. albus "Clade C"—imported from Vietnam and present in a U.S. retail food market revealed that 18 (95%) contained macroparasites. The 394 individual parasites recovered included a mix of nematodes, acanthocephalans, cestodes, digeneans, and pentastomes. The findings raise concern because of the likelihood that some parasites infecting market swamp eels imported from Asia are themselves Asian taxa, some possibly new to North America. The ecological risk is exacerbated because swamp eels sold in food markets are occasionally retained live by customers and a few reportedly released into the wild. For comparative purposes, M. albus "Clade C" swamp eels from a non-native population in Florida (USA) were also examined and most (84%) were found to be infected with internal macroparasites. The current level of analysis does not allow us to confirm whether these are non-native parasites.

Aquatic Invasions↗

Spatial and temporal patterns in streamflow, water chemistry, and aquatic macroinvertebrates of selected streams in Fairfax County, Virginia, 2007–18

Urbanization substantially alters the landscape in ways that can impact stream hydrology, water chemistry, and the health of aquatic communities. Stormwater best management practices (BMPs) are the primary tools used to mitigate the effects of urban stressors such as increased runoff, decreased baseflow, and increased nutrient and sediment transport. To date, Fairfax County Virginia’s stormwater management program has made substantial investments into the implementation of both structural and nonstructural BMPs aimed at restoring and protecting watersheds. The U.S. Geological Survey (USGS), in cooperation with Fairfax County, Virginia, established a long-term water-resources monitoring program to evaluate the watershed-scale effects of these investments. Monitoring began at 14 stations in 2007 and was expanded to 20 stations in 2013. This report utilized the first 10 years of data collection to (1) assess water quantity and quality, as well as ecological condition; (2) compute annual nutrient and sediment loads; and (3) evaluate trends in streamflow, water quality, and ecological condition. Efforts are underway to link the biotic and abiotic patterns described herein to watershed management practices as well as factors such as land use change, public works infrastructure, and climate. Hydrologic, chemical, and benthic macroinvertebrate community conditions in the streams monitored were similar to those observed in other studies of urban streams. Multidecadal trends in baseflow indices and runoff ratios at long-term Chesapeake Bay Non-tidal Network streamgages (CB-NTN) indicate a decrease in groundwater recharge and increase in storm runoff as a result of urbanization. Streamflow yields varied spatially with land cover, geology, and soil characteristics, whereas flashiness was positively related to impervious area. Dissolved oxygen typically was lowest in the Coastal Plain and across all Triassic Lowlands streams, and highest in the Piedmont. Dissolved oxygen concentrations generally were above Virginia’s minimum criterion of 4.0 milligrams per liter (mg/L), most violations occurred at Paul Spring Branch in the Coastal Plain during the warmest months of the year owing to increased chemical and biological oxygen demand. Typical pH values of the monitored streams centered on neutrality (pH = 7); however, diurnal fluctuations were most prevalent in the continuous pH data at Flatlick Branch (FLAT; a Triassic Lowlands station), as a result of increased photosynthesis catalyzed by phosphorus-rich geology. Specific conductance (SC) varied spatially owing to geology (highest at Triassic Lowlands stations) and anthropogenic disturbance (watersheds with high impervious land cover). Specific conductance typically was inversely related to streamflow except in winter months following deicing road salt applications, when values increased by several orders of magnitude. A significant increase in SC of about 2 percent per year was observed from the combined trend result of all monitoring stations over the 10-year period. Significant SC increases occurred at nearly all monitoring stations. Increasing trends were observed during winter and nonwinter months, which suggests that salts applied to deice roadways and other impervious surfaces are stored in the environment and released year-round. Suspended-sediment (SS) concentrations in monthly samples did not vary significantly between most stations, but typically were highest in the spring and lowest in the fall as a result of seasonal differences in streamflow and climate. Suspended-sediment yields ranged from 62 to 1,428 tons per square mile (ton/mi 2 ), with a median of 302 ton/mi 2 . Annual loads were greatest during the wettest water years (October 1-September 30; 2008, 2011, and 2014), with the greatest interannual variability occurring at Difficult Run above Fox Lake (DIFF) and South Fork Little Difficult Run (SFLIL). Suspended sediment was primarily composed of silts and clays; however, the proportion of sand in suspended sediment was related positively to streamflow. Cross-correlation analyses suggested the dominant sources of SS were streambank erosion and resuspension of in-channel material at DIFF and FLAT; whereas, upland sources and erosion of upper streambanks were more common at Dead Run (DEAD), Long Branch (LONG), and SFLIL. Median total phosphorus (TP) concentrations ranged from 0.016 to 0.077 mg/L, with a networkwide median of 0.022 mg/L, were highest in the warm season (April-September), and were composed primarily of dissolved phosphorous. Although TP concentrations were relatively low across the network, the highest concentrations were consistently at stations located in the Triassic Lowlands, owing to phosphorous-rich geology, and in the Coastal Plain, owing to the low-phosphorous sorptive capacity of those soils. A significant increase in TP concentration occurred in a few stations, but the combined trend results from all stations demonstrated a significant increase of about 4 percent per year. Networkwide increases were also observed in total dissolved phosphorus, orthophosphate, and total particulate phosphorus. The composition of TP shifted from dissolved to particulate as streamflow increased and for this reason loads primarily were composed of particulate phosphorous. Median annual TP loads were highest at FLAT and DEAD and ranged from 247 to 642 pounds per square mile (lbs/mi 2 ) networkwide. Interannual variability in phosphorous yields was apparent at most stations; the highest loading years were also the wettest years during the study period and coincident with the highest peak annual flows. Total nitrogen (TN) concentrations typically were low throughout the network with exceptions occurring at stations located in watersheds with a high density of septic infrastructure. Elevated TN concentrations also were observed in some watersheds without a high density of septic systems and may be attributable to geologic and soil properties that limit denitrification as well as other unknown anthropogenic inputs. Total nitrogen typically was dominated by nitrate during baseflows; however, the proportion of particulate nitrogen increased during stormflows. Total nitrogen yields were similar across stations, with medians ranging from about 3,600 to 6,300 lbs/mi 2 and were related to annual streamflow volume. Total nitrogen concentrations and flow-normalized concentrations decreased over the 10-year period at 7 stations, with median reductions of about 2.5 percent. Increasing trends were observed at the two stations with the highest median TN concentration (Captain Hickory Run and SFLIL, 3–5 mg/L), both watersheds contain a high density of septic infrastructure. The combined trend results from all stations revealed no trend in TN and a declining trend in nitrate of about 2 percent per year. Overall, benthic community metrics indicated that streams throughout Fairfax County were initially of poor health; however, many metrics show an improving trend (from poor to fair based on the Fairfax County Index of Biological Integrity [IBI]). Significant increasing trends in IBI occurred at the network-scale and at 4 individual stations; additionally, scores improved by at least 1 qualitative category (for example, poor to fair, fair to good) at 11 of the 14 stations between 2009 (the first year all 14 stations were sampled) and 2017. Changes in all metrics suggest that the biodiversity, function, and condition of streams in Fairfax County are improving, but some of these improvements are driven by increased diversity and percent composition of organisms that are tolerant of the urban environment.

Virginia↗

Changes in hydrodynamics and wave energy as a result of seagrass decline along the shoreline of a microtidal back-barrier estuary

Seagrasses are marine flowering plants that provide key ecological services. In recent decades, multiple stressors have caused a worldwide decline in seagrass beds. Changes in bottom friction associated with seagrass loss are expected to influence the ability of estuarine systems to trap sediment inputs through local and regional changes in hydrodynamics. Herein, we conduct a numerical study using six historical maps of seagrass distribution in Barnegat Bay, USA, to demonstrate that reductions in seagrass coverage destabilize estuarine systems, decreasing their flood-dominance in the areas interested by seagrass disappearance and increasing bed-shear stress values across the entire back-barrier basin. Furthermore, we reveal how seagrass decline has considerably increased the impact of wind-waves on marsh edges between 1968 and 2009. From a comparison with a numerical experiment without submerged aquatic vegetation, we estimate that up to 40% of the computed wave thrust on marsh boundaries can be reduced by seagrass beds and we find that the location of a seagrass patch in addition to its aerial extent plays a crucial role in this attenuation process. This study highlights the benefits of seagrass meadows in enhancing estuarine resilience and reducing marsh-edge retreat by wind-wave attack, which is recognized as a chief agent in lateral marsh loss.

Advances in Water Resources↗

Responses of plankton communities to the introduction of grass carp into some Georgia ponds

Net plankton community structure and numbers were studied in soft-water, acidic ponds containing aquatic macrophytes, after introduction of the herbivorous grass carp ( Ctenopharyngodon idella ). The plankton communities in ponds with grass carp consisted of significantly fewer individuals, genera, and orders than did the communities in control ponds. Expected shifts from desirable to undesirable species of plankton did not occur. These results demonstrate that plankton blooms are not an inevitable result of stocking grass carp, and support the suggestion that macrophytes may be especially significant in nutrient cycling in soft-water, acidic systems.

Georgia↗

Graptemys pearlensis Ennen, Lovich, Kreiser, Selman, and Qualls 2010 – Pearl River Map Turtle

The Pearl River Map Turtle, Graptemys pearlensis (Family Emydidae), is a moderate-sized aquatic turtle endemic to the Pearl River drainage of Louisiana and Mississippi. This taxon has long been a cryptic species, as it was considered part of G. pulchra before 1992 and part of G. gibbonsi until 2010. Graptemys pearlensis exhibits sexual dimorphism, with adult females being considerably larger (carapace length to 295 mm) than adult males (CL to 121 mm). In the 1960s and 1970s, the species was commonly found in higher abundance than the sympatric G. oculifera, a federally listed species. However, due to habitat degradation and the precipitous decline of native mollusks, the species is now found in lower numbers than G. oculifera throughout much of its range. The current IUCN Red List status is Endangered; however, very little is known about the natural history and ecology of the species, which will make conservation efforts challenging.

Book chapter↗

Methods for measuring denitrification: Diverse approaches to a difficult problem

Denitrification, the reduction of the nitrogen (N) oxides, nitrate (NO 3 − ) and nitrite (NO 2 − ), to the gases nitric oxide (NO), nitrous oxide (N 2 O), and dinitrogen (N 2 ), is important to primary production, water quality, and the chemistry and physics of the atmosphere at ecosystem, landscape, regional, and global scales. Unfortunately, this process is very difficult to measure, and existing methods are problematic for different reasons in different places at different times. In this paper, we review the major approaches that have been taken to measure denitrification in terrestrial and aquatic environments and discuss the strengths, weaknesses, and future prospects for the different methods. Methodological approaches covered include (1) acetylene-based methods, (2) 15 N tracers, (3) direct N 2 quantification, (4) N 2 :Ar ratio quantification, (5) mass balance approaches, (6) stoichiometric approaches, (7) methods based on stable isotopes, (8) in situ gradients with atmospheric environmental tracers, and (9) molecular approaches. Our review makes it clear that the prospects for improved quantification of denitrification vary greatly in different environments and at different scales. While current methodology allows for the production of accurate estimates of denitrification at scales relevant to water and air quality and ecosystem fertility questions in some systems (e.g., aquatic sediments, well-defined aquifers), methodology for other systems, especially upland terrestrial areas, still needs development. Comparison of mass balance and stoichiometric approaches that constrain estimates of denitrification at large scales with point measurements (made using multiple methods), in multiple systems, is likely to propel more improvement in denitrification methods over the next few years.

Ecological Applications↗

Trophic ontogeny of fluvial Bull Trout and seasonal predation on Pacific Salmon in a riverine food web

Bull Trout Salvelinus confluentus are typically top predators in their host ecosystems. The Skagit River in northwestern Washington State contains Bull Trout and Chinook Salmon Oncorhynchus tshawytscha populations that are among the largest in the Puget Sound region and also contains a regionally large population of steelhead O. mykiss (anadromous Rainbow Trout). All three species are listed as threatened under the Endangered Species Act (ESA). Our objective was to determine the trophic ecology of Bull Trout, especially their role as predators and consumers in the riverine food web. We seasonally sampled distribution, diets, and growth of Bull Trout in main-stem and tributary habitats during 2007 and winter–spring 2008. Consumption rates were estimated with a bioenergetics model to (1) determine the annual and seasonal contributions of different prey types to Bull Trout energy budgets and (2) estimate the potential impacts of Bull Trout predation on juvenile Pacific salmon populations. Salmon carcasses and eggs contributed approximately 50% of the annual energy budget for large Bull Trout in main-stem habitats, whereas those prey types were largely inaccessible to smaller Bull Trout in tributary habitats. The remaining 50% of the energy budget was acquired by eating juvenile salmon, resident fishes, and immature aquatic insects. Predation on listed Chinook Salmon and steelhead/Rainbow Trout was highest during winter and spring (January–June). Predation on juvenile salmon differed between the two study years, likely due to the dominant odd-year spawning cycle for Pink Salmon O. gorbuscha . The population impact on ocean- and stream-type Chinook Salmon was negligible, whereas the impact on steelhead/Rainbow Trout was potentially very high. Due to the ESA-listed status of Bull Trout, steelhead, and Chinook Salmon, the complex trophic interactions in this drainage provide both challenges and opportunities for creative adaptive management strategies.

Washington↗

Population genetics reveals bidirectional fish movement across the Continental Divide via an interbasin water transfer

Interbasin water transfers are becoming an increasingly common tool to satisfy municipal and agricultural water demand, but their impacts on movement and gene flow of aquatic organisms are poorly understood. The Grand Ditch is an interbasin water transfer that diverts water from tributaries of the upper Colorado River on the west side of the Continental Divide to the upper Cache la Poudre River on the east side of the Continental Divide. We used single nucleotide polymorphisms to characterize population genetic structure in cutthroat trout ( Oncorhynchus clarkii ) and determine if fish utilize the Grand Ditch as a movement corridor. Samples were collected from two sites on the west side and three sites on the east side of the Continental Divide. We identified two or three genetic clusters, and relative migration rates and spatial distributions of admixed individuals indicated that the Grand Ditch facilitated bidirectional fish movement across the Continental Divide, a major biogeographic barrier. Previous studies have demonstrated ecological impacts of interbasin water transfers, but our study is one of the first to use genetics to understand how interbasin water transfers affect connectivity between previously isolated watersheds. We also discuss implications on native trout management and balancing water demand and biodiversity conservation.

Colorado↗