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Challenges of establishing big sgebrush ( Artemisia tridentata ) in rangeland restoration: effects of herbicide, mowing, whole-community seeding, and sagebrush seed sources

The loss of big sagebrush (Artemisia tridentata Nutt.) on sites disturbed by fire has motivated restoration seeding and planting efforts. However, the resulting sagebrush establishment is often lower than desired, especially in dry areas. Sagebrush establishment may be increased by addressing factors such as seed source and condition or management of the plant community. We assessed initial establishment of seeded sagebrush and four populations of small outplants (from different geographies, climates, and cytotypes) and small sagebrush outplants in an early seral community where mowing, herbicide, and seeding of other native plants had been experimentally applied. No emergence of seeded sagebrush was detected. Mowing the site before planting seedlings led to greater initial survival probabilities for sagebrush outplants, except where seeding also occurred, and these effects were related to corresponding changes in bare soil exposure. Initial survival probabilities were > 30% greater for the local population of big sagebrush relative to populations imported to the site from typical seed transfer distances of ~320–800 km. Overcoming the high first-year mortality of outplanted or seeded sagebrush is one of the most challenging aspects of postfire restoration and rehabilitation, and further evaluation of the impacts of herb treatments and sagebrush seed sources across different site types and years is needed.

Rangeland Ecology and Management

A quantitative soil-geomorphic framework for developing and mapping ecological site groups

Land management decisions need context about how landscapes will respond to different circumstances or actions. As ecologists’ understanding of nonlinear ecological dynamics has evolved into state-and-transition models (STMs), they have put more emphasis on defining and mapping the soil, geomorphological, and climate parameters that mediate these dynamics. The US Department of Agriculture Natural Resources Conservation Service ecological site descriptions (ESDs) have become the foremost system in classifying lands into ecological units based on STMs. However, an exhaustive inventory of ESDs has proved challenging to complete in the United States, and there have been questions about the consistency of detail in areas completed and the ability to objectively support some assertions made in existing ESDs. To address these issues, this study examines ESDs in the diverse Upper Colorado River region, where ESDs are only partially complete, to look at quantitative approaches to generalizing ecological site concepts based on unifying underlying soil, geomorphology, and climate patterns. Using existing ESDs and vegetation monitoring plot data, results show that a simple hierarchical soil geomorphic unit (SGU) framework based on topographic mediation of moisture, soil salinity, soil depth, slope, rock content, and soil texture can represent much of the ecological dynamics cataloged in ESDs. Analyses of reference plant production data, ecological state attribution, and regional monitoring data show that the new SGUs represent more variation than common climate parameters. This study also included predictively mapping SGUs at 30-m resolution (Kappa of 0.53, 74% agreement with top two predictions in validation). An optimized combination of SGUs with climate zones derived from an aridity index and maximum temperature of the hottest month resulted in an ecological site group framework that condensed over 826 unique ecological site records at various stages of completeness in the regional soil survey down to 35 intuitive and mappable ecological site groups.

Rangeland Ecology and Management

Spatial variation in effectiveness and costs of sagebrush restoration treatments across the western United States

Natural resource managers strive to improve restoration efficiency across the vast network of public lands of the United States, seeking the best return on investment through innovation, geographic prioritization, and adaptive management. This challenge is exemplified by ecosystems dominated by sagebrush ( Artemisia spp.), which occur across the western United States and have been degraded by increasing wildfire frequency driven by invasive plant spread, among other factors. However, both costs and effectiveness of sagebrush restoration can vary spatially due to management practices in addition to biotic and abiotic factors, and characterizing this variation could inform broad-scale planning. We leveraged published models of sagebrush recovery and treatment costs to predict and evaluate cost-effectiveness of aerial and drill seeding Artemisia spp. across 429 718 km 2 that overlapped 12 US states. Compared to natural recovery, effectiveness for both methods was generally low, yet effectiveness was often greater, and costs were always greater, for drill than aerial seeding, resulting in slightly higher cost-effectiveness on average for drill seeding. Cost-effectiveness for both treatments increased near major roads and in areas with more repeated burns. Cost-effectiveness also increased with predisturbance cover of sagebrush but decreased with herbaceous cover. However, we also identified areas where aerial seeding was more cost-effective than drill seeding, particularly with greater slope. Our results provide spatially explicit estimates and potential mechanisms of cost-effectiveness for two common seeding methods for sagebrush, which can help prioritize limited resources, guide land use, and improve restoration efficiency and effectiveness across public rangelands of the western United States.

western United States

Pinyon and juniper encroachment into sagebrush ecosystems impacts distribution and survival of greater sage-grouse

In sagebrush ( Artemisia spp.) ecosystems, encroachment of pinyon ( Pinus spp.) and juniper ( Juniperus spp.; hereafter, “pinyon-juniper”) trees has increased dramatically since European settlement. Understanding the impacts of this encroachment on behavioral decisions, distributions, and population dynamics of greater sage-grouse (Centrocercus urophasianus) and other sagebrush obligate species could help benefit sagebrush ecosystem management actions. We employed a novel two-stage Bayesian model that linked avoidance across different levels of pinyon-juniper cover to sage-grouse survival. Our analysis relied on extensive telemetry data collected across 6 yr and seven subpopulations within the Bi-State Distinct Population Segment (DPS), on the border of Nevada and California. The first model stage indicated avoidance behavior for all canopy cover classes on average, but individual grouse exhibited a high degree of heterogeneity in avoidance behavior of the lowest cover class (e.g., scattered isolated trees). The second stage modeled survival as a function of estimated avoidance parameters and indicated increased survival rates for individuals that exhibited avoidance of the lowest cover class. A post hoc frailty analysis revealed the greatest increase in hazard (i.e., mortality risk) occurred in areas with scattered isolated trees consisting of relatively high primary plant productivity. Collectively, these results provide clear evidence that local sage-grouse distributions and demographic rates are influenced by pinyon-juniper, especially in habitats with higher primary productivity but relatively low and seemingly benign tree cover. Such areas may function as ecological traps that convey attractive resources but adversely affect population vital rates. To increase sage-grouse survival, our model predictions support reducing actual pinyon-juniper cover as low as 1.5%, which is lower than the published target of 4.0%. These results may represent effects of pinyon-juniper cover in areas with similar ecological conditions to those of the Bi-State DPS, where populations occur at relatively high elevations and pinyon-juniper is abundant and widespread.

Rangeland Ecology and Management

Performance of quantitative vegetation sampling methods across gradients of cover in Great Basin plant communities

Resource managers and scientists need efficient, reliable methods for quantifying vegetation to conduct basic research, evaluate land management actions, and monitor trends in habitat conditions. We examined three methods for quantifying vegetation in 1-ha plots among different plant communities in the northern Great Basin: photography-based grid-point intercept (GPI), line-point intercept (LPI), and point-quarter (PQ). We also evaluated each method for within-plot subsampling adequacy and effort requirements relative to information gain. We found that, for most functional groups, percent cover measurements collected with the use of LPI, GPI, and PQ methods were strongly correlated. These correlations were even stronger when we used data from the upper canopy only (i.e., top “hit” of pin flags) in LPI to estimate cover. PQ was best at quantifying cover of sparse plants such as shrubs in early successional habitats. As cover of a given functional group decreased within plots, the variance of the cover estimate increased substantially, which required more subsamples per plot (i.e., transect lines, quadrats) to achieve reliable precision. For GPI, we found that that six–nine quadrats per hectare were sufficient to characterize the vegetation in most of the plant communities sampled. All three methods reasonably characterized the vegetation in our plots, and each has advantages depending on characteristics of the vegetation, such as cover or heterogeneity, study goals, precision of measurements required, and efficiency needed.

Idaho;Oregon

Detecting channel riparian vegetation response to best-management-practices implementation in ephemeral streams with the use of spot high-resolution visible imagery

Heavily grazed riparian areas are commonly subject to channel incision, a lower water table, and reduced vegetation, resulting in sediment delivery above normal regimes. Riparian and in-channel vegetation functions as a roughness element and dissipates flow energy, maintaining stable channel geometry. Ash Creek, a tributary of the Bad River in western South Dakota contains a high proportion of incised channels, remnants of historically high grazing pressure. Best management practices (BMP), including off-stream watering sources and cross fencing, were implemented throughout the Bad River watershed during an Environmental Protection Agency (EPA) 319 effort to address high sediment loads. We monitored prairie cordgrass (Spartina pectinata Link) establishment within stream channels for 16 yr following BMP implementation. Photos were used to group stream reaches (n = 103) subjectively into three classes; absent (estimated < 5% cover; n = 64), present (estimated 5–40% cover; n = 23), and dense (estimated > 40% cover; n = 16) based on the relative amount of prairie cordgrass during 2010 assessments of ephemeral channels. Reaches containing drainage areas of 0.54 to 692 ha were delineated with the use of 2010 National Agriculture Imagery Program (NAIP) imagery. Normalized difference vegetation index (NDVI) values were extracted from 5 to 39 sample points proportional to reach length using a series of Satellite Pour l'Observation de la Terre (SPOT) satellite imagery. Normalized NDVI (nNDVI) of 2 152 sample points were determined from pre- and post-BMP images. Mean nNDVI values for each reach ranged from 0.33 to 1.77. ANOVA revealed significant increase in nNDVI in locations classified as present prairie cordgrass cover following BMP implementation. Establishment of prairie cordgrass following BMP implementation was successfully detected remotely. Riparian vegetation such as prairie cordgrass adds channel roughness that reduces the flow energy responsible for channel degradation.

South Dakota

Predictive models of selective cattle use of large, burned landscapes in semiarid sagebrush-steppe

The fire-exotic annual grass cycle is a severe threat to shrub-steppe rangelands, and a greater understanding of how livestock grazing relates to the problem is needed to guide effective management interventions. Grazing effects vary throughout shrub-steppe rangelands because livestock are selective in their use within pastures. Thus, knowing where cattle are located and concentrate their use in a postfire landscape is important for enhancing plant community resiliency to disturbance and resistance to exotic annual grass invasion. We asked how the distribution and intensity of cattle use varied across 113 000 ha of recently burned, environmentally varied shrub-steppe. Generalized linear mixed effects models were used to determine the relationship of cattle dung (presence/absence and counts), which was recorded during the third to fifth postfire year (after grazing deferment) on 1166 (531-m 2 ) plots, to water sources, burn severity, grass cover, and topographic predictors. Our distribution and intensity of use models revealed similar relationships between cattle use and landscape predictors. Cattle use was greater in areas that were flatter and closer to water and that had moderate burn severity and less heat load and ruggedness. Slope had the strongest effect on cattle use of the predictors. The probability of cattle being present decreased by 10% for every 5° increase in slope until slope exceeded 15°, and then the effect of slope weakened. Despite moderate slopes &#x3C7; &#xAF; "> χ¯ = 14°), cattle use was greater in areas of moderate burn severity, presumably because these areas provided greater perennial grass production. While there was much unexplained variation, these models suggest that cooler climate, water access, topographic factors, and burn severity affect maneuverability to create greater livestock use of certain areas within grazing pastures. Restoration investment planning or assessments and expectations of restoration success could be improved by considering that these livestock hotspots may recover differently from the surrounding landscape.

Rangeland Ecology and Management

Appropriate sample sizes for monitoring burned pastures in sagebrush steppe: How many plots are enough, and can one size fit all?

Statistically defensible information on vegetation conditions is needed to guide rangeland management decisions following disturbances such as wildfire, often for heterogeneous pastures. Here we evaluate the number of plots needed to make informed adaptive management decisions using >2000 plots sampled on the 2015 Soda Fire that burned across 75 pastures and 113,000 ha in Idaho and Oregon, USA. We predicted that the number of plots required to generate a threshold of standard error/mean ≤0.2 (TSR, threshold sampling requirement) for plant cover within pasture units would vary between sampling methods (rapid ocular versus grid-point intercept) and among plot sizes (1, 6, or 531 m2), as well as relative to topography, elevation, pasture size, complexity of soils and vegetation treatments applied, and dominance by exotic annual or perennial grasses. Sampling was adequate for determining exotic annual and perennial grass cover in about half of the pastures. A tradeoff in number versus size of plots sampled was apparent, whereby TSR was attainable with less area searched using smaller plot sizes (1 compared to 531 m2) in spite of less variability between larger plots. TSR for both grass types decreased as their dominance increased (0.5-1.5 plots per % cover increment). TSR decreased for perennial grass but increased for exotic annual grass with higher elevations. TSR increased with standard deviation of elevation for perennial grass sampled with grid-point intercept. Sampling effort could be more reliably predicted from landscape variables for the grid-point compared to ocular sampling method. These findings suggest that adjusting the number and size of sample plots within a pasture or burn area using easily determined landscape variables could increase monitoring efficiency and effectiveness.

Rangeland Ecology and Management

An introduction and practical guide to use of the Soil-Vegetation Inventory Method (SVIM) data

Long-term vegetation dynamics across public rangelands in the western United States are not well understood because of the lack of large-scale, readily available historic datasets. The Bureau of Land Management’s Soil-Vegetation Inventory Method (SVIM) program was implemented between 1977 and 1983 across 14 western states, but the data have not been easily accessible. We introduce the SVIM vegetation cover dataset in a georeferenced, digital format; summarize how the data were collected; and discuss potential limitations and biases. We demonstrate how SVIM data can be compared with contemporary monitoring datasets to quantify changes in vegetation associated with wildfire and the abundance of exotic invasive species. Specifically, we compare SVIM vegetation cover data with cover data collected by BLM’s Assessment, Inventory, and Monitoring (AIM) program (2011–2016) in a focal area in the northern Great Basin. We address issues associated with analyzing and interpreting data from these distinct programs, including differences in survey methods and potential biases introduced by spatial and temporal variation in sampling. We compared SVIM and AIM survey methods at 44 plots and found that percent cover estimates had high correspondence for all measured functional groups. Comparisons between historic SVIM data and recent AIM data documented significant declines in the occupancy and cover of native shrubs and native perennial forbs , and a significant increase in exotic annual forbs. Wildfire was a driver of change for some functional groups, with greater change occurring in AIM plots that burned between the two time periods compared with those that did not. Our results are consistent with previous studies showing that many native shrub-dominated plant communities in the Great Basin have been replaced by exotic annuals. Our study demonstrates that SVIM data will be an important resource for researchers interested in quantifying vegetation change through time across public rangelands in the western United States.

Rangeland Ecology and Management

Post-fire management-scale trials of bacterial soil amendment MB906 show inconsistent control of invasive annual grasses

Rangeland managers need tools to control invasive annual grasses, particularly following wildfire. We assessed responses of native and invasive/exotic grasses to the MB906 soil amendment containing live cultures of a purportedly weed-suppressive strain of the bacterium Pseudomonas fluorescens (“WSB”). MB906 was applied alone and in combination with the pre-emergent herbicide imazapic on >3000 ha across three sagebrush-steppe landscapes burned several months prior. Replicate plots of each treatment type were established and plant cover was measured in the following three years. Cover of invasive-annual grasses (“IAG”) was not responsive to MB906 when all IAG species were considered (“IAG-All”). However, MB906 led to a 54% reduction in the IAG's that were previously reported to be controlled by WSB (“IAG-Target”) in the second year following application (IAG-Target = cheatgrass, Bromus tectorum and medusahead, Taeniatherum caput-medusae; IAG-All also includes Vulpia myuros and Bromus arvensis ). MB906 reduced the effectiveness of co-applied imazapic: Imazapic alone reduced IAG-All by 83% and 68% in years 1 and 2, respectively, while imazapic+MB906 reduced IAG-All by 48% and 38% in years 1 and 2, respectively, across all landscapes, and a similar response pattern was observed for IAG-Target. Perennial grass cover was unaffected by the treatments except where it increased 4-fold in response to imazapic applied at a high rate (0.140 kg a.i. ha −1 ) in one of the landscapes. Tank mixing MB906 and herbicide may have lessened the biological activity of the herbicide by altering the pH or mineral content of the spray solution or by direct metabolism of the herbicide by the bacteria. These results do not provide strong support for MB906 as a tool for annual grass control, though they suggest further investigation may be warranted.

Rangeland Ecology and Management

Transition of vegetation states positively affects harvester ants in the Great Basin, United States

Invasions by non-native plants can alter ecosystems such that new ecological states are reached, but less is known about how these transitions influence animal populations. Sagebrush (Artemisia tridentata) ecosystems are experiencing state changes because of fire and invasion by exotic annual grasses. Our goal was to study the effects of these state changes on the Owyhee and western harvester ants ( Pogonomyrmex salinus Olsen and P. occidentalis Cresson, respectively). We sampled 358 1-ha plots across the northern Great Basin, which captured unburned and burned conditions across 1 −≥31 years postfire. Our results indicated an immediate and consistent change in vegetation states from shrubland to grassland between 1 and 31 years postfire. Harvester ant occupancy was unrelated to time since fire, whereas we observed a positive effect of fire on nest density. Similarly, we discovered that fire and invasion by exotic annuals were weak predictors of harvester ant occupancy but strong predictors of nest density. Occupancy of harvester ants was more likely in areas with finer-textured soils, low precipitation, abundant native forbs, and low shrub cover. Nest density was higher in arid locations that recently burned and exhibited abundant exotic annual and perennial (exotic and native) grasses. Finally, we discovered that burned areas that received postfire restoration had minimal influence on harvester ant occupancy or nest density compared with burned and untreated areas. These results suggest that fire-induced state changes from native shrublands to grasslands dominated by non-native grasses have a positive effect on density of harvester ants (but not occupancy), and that postfire restoration does not appear to positively or negatively affect harvester ants. Although wildfire and invasion by exotic annual grasses may negatively affect other species, harvester ants may indeed be one of the few winners among a myriad of losers linked to vegetation state changes within sagebrush ecosystems.

Great Basin

Encounters with Pinyon-Juniper influence riskier movements in Greater Sage-Grouse across the Great Basin

Fine-scale spatiotemporal studies can better identify relationships between individual survival and habitat fragmentation so that mechanistic interpretations can be made at the population level. Recent advances in Global Positioning System (GPS) technology and statistical models capable of deconstructing high-frequency location data have facilitated interpretation of animal movement within a behaviorally mechanistic framework. Habitat fragmentation due to singleleaf pinyon (Pinus monophylla; hereafter pinyon) and Utah juniper (Juniperus osteosperma; hereafter juniper) encroachment into sagebrush (Artemisia spp.) communities is a commonly implicated perturbation that can adversely influence greater sage-grouse (Centrocercus urophasianus; hereafter sage-grouse) demographic rates. Using an extensive GPS data set (233 birds and 282,954 locations) across 12 study sites within the Great Basin, we conducted a behavioral change point analysis and subsequently constructed Brownian bridge movement models from each behaviorally homogenous section. We found a positive relationship between modeled movement rate and probability of encountering pinyon-juniper with significant variation among age classes. The probability of encountering pinyon-juniper among adults was two and three times greater than that of yearlings and juveniles, respectively. However, the movement rate in response to the probability of encountering pinyon-juniper trees was 1.5 times greater for juveniles. We then assessed the risk of mortality associated with an interaction between movement rate and the probability of encountering pinyon-juniper using shared frailty models. During pinyon-juniper encounters, on average, juvenile, yearling, and adult birds experienced a 10.4%, 0.2%, and 0.3% reduction in annual survival probabilities. Populations that used pinyon-juniper habitats with a frequency ≥ 3.8 times the overall mean experienced decreases in annual survival probabilities of 71.1%, 0.9%, and 0.9%. This analytical framework identifies a likely behavioral mechanism behind how pinyon-juniper encroachment decreases habitat suitability for sage-grouse, whereby encountering pinyon-juniper stimulates faster yet riskier movements that may make sage-grouse more vulnerable to visually acute predators.

Rangeland Ecology and Management

Lesser prairie-chicken avoidance of trees in a grassland landscape

Grasslands are among the most imperiled ecosystems in North America. Reasons that grasslands are threatened include conversion to row-crop agriculture, fragmentation, and changes in fire regimes. The reduction of fire processes in remaining prairies has resulted in tree encroachment and establishment in grasslands, further reducing grassland quantity and quality. Grassland birds have been experiencing precipitous population declines in recent decades, commensurate with landscape changes to grasslands. The lesser prairie-chicken ( Tympanuchus pallidicinctus Ridgway) is a declining species of prairie grouse of conservation concern. We used second- and third-order habitat selection metrics to test if female lesser prairie-chickens avoid grasslands where trees were present. Our results indicated that female lesser prairie-chickens selected habitats avoiding the nearest trees by 283 m on average, nearly twice as far as would be expected at random. Lesser prairie-chickens were 40 times more likely to use habitats with tree densities of 0 trees ∙ ha − 1 than habitats with 5 trees ∙ ha − 1 . Probability of use indicated that lesser prairie-chickens were 19 times more likely to use habitats 1000 m from the nearest tree when compared with using habitats 0 m from the nearest tree. Nest survival was not affected at densities < 2 trees ∙ ha − 1 ; however, we could not test if nest survival was affected at greater tree densities as no nests were detected at densities > 2 trees ∙ ha − 1 . Avoidance of trees could be due to perceived increased predation risk, reduced habitat quality, or a combination of these potentially confounding factors. Preventing further establishment and expansion of trees in landscapes occupied by lesser prairie-chickens could contribute to the continued persistence of the species. Additionally, restoring grasslands through tree removal may facilitate conservation efforts for grassland species such as the lesser prairie-chicken by improving habitat quality and promoting expansion of occupied range.

Kansas

Mapping tree canopy cover in support of proactive prairie grouse conservation in western North America

Invasive woody plant expansion is a primary threat driving fragmentation and loss of sagebrush ( Artemisia spp.) and prairie habitats across the central and western United States. Expansion of native woody plants, including conifer (primarily Juniperus spp.) and mesquite ( Prosopis spp.), over the past century is primarily attributable to wildfire suppression, historic periods of intensive livestock grazing, and changes in climate. To guide successful conservation programs aimed at reducing top-down stressors, we mapped invasive woody plants at regional scales to evaluate landscape level impacts, target restoration actions, and monitor restoration outcomes. Our overarching goal was to produce seamless regional products across sociopolitical boundaries with resolution fine enough to depict the spatial extent and degree of woody plant invasion relevant to greater sage-grouse (Centrocercus urophasianus) and lesser prairie-chicken (Tympanuchus pallidicinctus) conservation efforts. We mapped tree canopy cover at 1-m spatial resolution across an 11-state region (508 265 km 2 ). Greater than 90% of occupied lesser prairie-chicken habitat was largely treeless for conifers (< 1% canopy cover), whereas > 67% was treeless for mesquite. Conifers in the higher canopy cover classes (16 − 50% and > 50% canopy cover) were scarce (< 2% and 1% canopy cover), as was mesquite (< 5% and 1% canopy cover). Occupied habitat by sage-grouse was more variable but also had a relatively large proportion of treeless areas ( x &#x2212; "> x− = 71, SE = 5%). Low to moderate levels of conifer cover (1 − 20%) were fewer ( x &#x2212; "> x− = 23, SE = 5%) as were areas in the highest cover class (> 50%; x &#x2212; "> x− = 6, SE = 2%). Mapping indicated that a high proportion of invading woody plants are at a low to intermediate level. Canopy cover maps for conifer and mesquite resulting from this study provide the first and most geographically complete, high-resolution assessment of woody plant cover as a top-down threat to western sage-steppe and prairie ecosystems.

Rangeland Ecology and Management

Impacts of mesquite distribution on seasonal space use of lesser prairie-chickens

Loss of native grasslands by anthropogenic disturbances has reduced availability and connectivity of habitat for many grassland species. A primary threat to contiguous grasslands is the encroachment of woody vegetation, which is spurred by disturbances that take on many forms from energy development, fire suppression, and grazing. These disturbances are exacerbated by natural- and human-driven cycles of changes in climate punctuated by drought and desertification conditions. Encroachment of honey mesquite (Prosopis glandulosa) into the prairies of southeastern New Mexico has potentially limited habitat for numerous grassland species, including lesser prairie-chickens (Tympanuchus pallidicinctus) . To determine the magnitude of impacts of distribution of mesquite and how lesser prairie-chickens respond to mesquite presence on the landscape in southeastern New Mexico, we evaluated seasonal space use of lesser prairie-chickens in the breeding and nonbreeding seasons. We derived several remotely sensed spatial metrics to characterize the distribution of mesquite. We then used these data to create population-level resource utilization functions and predict intensity of use of lesser prairie-chickens across our study area. Home ranges were smaller in the breeding season compared with the nonbreeding season; however, habitat use was similar across seasons. During both seasons, lesser prairie-chickens used areas closer to leks and largely avoided areas with mesquite. Relative to the breeding season, during the nonbreeding season habitat use suggested a marginal increase in mesquite within areas of low intensity of use, yet aversion to mesquite was strong in areas of medium to high intensity of use. To our knowledge, our study is the first to demonstrate a negative behavioral response by lesser prairie-chickens to woody encroachment in native grasslands. To mitigate one of the possible limiting factors for lesser prairie-chickens, we suggest future conservation strategies be employed by land managersto reduce mesquite abundance in the southern portion of their current range.

New Mexico

Productivity and CO 2 exchange of Great Plains ecoregions. I. Shortgrass steppe: Flux tower estimates

The shortgrass steppe (SGS) occupies the southwestern part of the Great Plains. Half of the land is cultivated, but significant areas remain under natural vegetation. Despite previous studies of the SGS carbon cycle, not all aspects have been completely addressed, including gross productivity, ecosystem respiration, and ecophysiological parameters. Our analysis of 1998 − 2007 flux tower measurements at five Bowen ratio–energy balance (BREB) and three eddy covariance (EC) sites characterized seasonal and interannual variability of gross photosynthesis and ecosystem respiration. Identification of the nonrectangular hyperbolic equation for the diurnal CO 2 exchange, with vapor pressure deficit (VPD) limitation and exponential temperature response, quantified quantum yield α , photosynthetic capacity A max , and respiration rate r d with variation ranges (19 \< α \ < 51 mmol mol − 1 , 0.48 \< A max \ < 2.1 mg CO 2 m − 2 s − 1 , 0.15 \< r d \ < 0.49 mg CO 2 m − 2 s − 1 ). Gross photosynthesis varied from 1 100 to 2 700 g CO 2 m − 2 yr − 1 , respiration from 900 to 3,000 g CO 2 m − 2 yr − 1 , and net ecosystem production from − 900 to + 700 g CO 2 m − 2 yr − 1 , indicating that SGS may switch from a sink to a source depending on weather. Comparison of the 2004 − 2006 measurements at two BREB and two parallel EC flux towers located at comparable SGS sites showed moderately higher photosynthesis, lower respiration, and higher net production at the BREB than EC sites. However, the difference was not related only to methodologies, as the normalized difference vegetation index at the BREB sites was higher than at the EC sites. Overall magnitudes and seasonal patterns at the BREB and the EC sites during the 3-yr period were similar, with trajectories within the ± 1.5 standard deviation around the mean of the four sites and mostly reflecting the effects of meteorology.

Colorado, Wyoming

Spectrophotometry of Artemisia tridentata to quantitatively determine subspecies

Ecological restoration is predicated on our abilities to discern plant taxa. Taxonomic identification is a first step in ensuring that plants are appropriately adapted to the site. An example of the need to identify taxonomic differences comes from big sagebrush (Artemisia tridentata). This species is composed of three predominant subspecies occupying distinct environmental niches, but overlap and hybridization are common in ecotones. Restoration of A. tridentata largely occurs using wildland collected seed, but there is uncertainty in the identification of subspecies or mix of subspecies from seed collections. Laboratory techniques that can determine subspecies composition would be desirable to ensure that subspecies match the restoration site environment. In this study, we use spectrophotometry to quantify chemical differences in the water-soluble compound, coumarin. Ultraviolet (UV) absorbance of A. tridentata subsp. vaseyana showed distinct differences among A.t. tridentata and wyomingensis. No UV absorbance differences were detected between A.t. tridentata and wyomingensis. Analyses of samples from > 600 plants growing in two common gardens showed that UV absorbance was unaffected by environment. Moreover, plant tissues (leaves and seed chaff) explained only a small amount of the variance. UV fluorescence of water-eluted plant tissue has been used for many years to indicate A.t. vaseyana; however, interpretation has been subjective. Use of spectrophotometry to acquire UV absorbance provides empirical results that can be used in seed testing laboratories using the seed chaff present with the seed to certify A. tridentata subspecies composition. On the basis of our methods, UV absorbance values < 2.7 would indicate A.t. vaseyana and values > 3.1 would indicate either A.t. tridentata or wyomingensis. UV absorbance values between 2.7 and 3.1 would indicate a mixture of A.t. vaseyana and the other two subspecies.

Rangeland Ecology and Management

Insights from long-term ungrazed and grazed watersheds in a salt desert Colorado Plateau ecosystem

Dryland ecosystems cover over 41% of the earth’s land surface, and living within these important ecosystems are approximately 2 billion people, a large proportion of whom are subsistence agropastoralists. Improper grazing in drylands can negatively impact ecosystem productivity, soil conservation, hydrologic processes, downstream water quantity and quality, and ultimately human health and economic well-being. Concerns regarding the degraded state of western US rangelands in the 1950s resulted in an interagency committee to study the effects of land use on runoff and erosion processes. In 1953, a federal research group established four paired watersheds in western Colorado to study the interaction of grazing by domestic livestock, runoff, and sediment yield. Exclusion of livestock from half of the watersheds dramatically reduced runoff and sediment yield after the first 10 yr—primarily due to changes in ground cover but not vegetation. Here, we report results of repeated soils and vegetation assessments of the experimental watersheds after more than 50 yr of grazing exclusion. Results show that many of the differences in soil conditions between grazed and ungrazed watersheds observed in the 1950s and 1960s were still present in 2004, despite reduced numbers of livestock: few differences in vegetation cover but large differences in biological soil crusts, soil stability, soil compaction, and soil biogeochemistry. There were differences among soil types in response to grazing history, especially soil lichen cover and soil organic matter, nitrogen, and sodium. Comparisons of ground cover measured in 2004 with those measured in 1953, 1966, and 1972 suggest much of the differences between grazed and ungrazed watersheds likely were driven by high sheep numbers during droughts in the 1950s. Persistence of these differences, despite large reductions in stocking rates , suggest the combination of overgrazing and drought may have pushed these salt desert ecosystems into a persistent, degraded ecological state.

Salt Desert Colorado Plateau Ecosystem