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Agricultural cropland extent and areas of South Asia derived using Landsat satellite 30-m time-series big-data using random forest machine learning algorithms on the Google Earth Engine cloud

The South Asia (India, Pakistan, Bangladesh, Nepal, Sri Lanka and Bhutan) has a staggering 900 million people (~43% of the population) who face food insecurity or severe food insecurity as per United Nations, Food and Agriculture Organization’s (FAO) the Food Insecurity Experience Scale (FIES). The existing coarse-resolution (>250-m) cropland maps lack precision in geo-location of individual farms and have low map accuracies. This also results in uncertainties in cropland areas calculated from such products. Thereby, the overarching goal of this study was to develop high spatial resolution (30-m or better) baseline cropland extent product of South Asia for the year 2015 using Landsat satellite time-series big-data and machine learning algorithms (MLAs) on the Google Earth Engine (GEE) cloud computing platform. To eliminate the impact of clouds, ten time-composited Landsat bands (blue, green, red, NIR, SWIR1, SWIR2, Thermal, EVI, NDVI, NDWI) were derived for each of the 3 time-periods over 12 months (monsoon: Julian days 151-300; winter: Julian days 301-365 plus 1-60; and summer: Julian days 61-150), taking the every 8-day data from Landsat-8 and 7 for the years 2013-2015, for a total of 30-bands plus global digital elevation model (GDEM) derived slope band. This 31-band mega-file big data-cube was composed for each of the 5 agro-ecological zones (AEZ’s) of South Asia and formed a baseline data for image classification and analysis. Knowledge-base for the Random Forest (RF) MLAs were developed using spatially well spread-out reference training data (N=2179) in 5 AEZs. Classification was performed on GEE for each of the 5 AEZs using well-established knowledge-based and RF MLAs on the cloud. Map accuracies were measured using independent validation data (N=1185). The survey showed that the South Asia cropland product had a producer’s accuracy of 89.9% (errors of omissions of 10.1%), user’s accuracy of 95.3% (errors of commission of 4.7%) and an overall accuracy of 88.7%. The National and sub-national (districts) areas computed from this cropland extent product explained 80-96% variability when compared with the National statistics of the South Asian Countries. The full resolution imagery can be viewed at full-resolution, by zooming-in to any location in South Asia or the world, at www.croplands.org and the cropland products of South Asia downloaded from The Land Processes Distributed Active Archive Center (LP DAAC) of National Aeronautics and Space Administration (NASA) and the United States Geological Survey (USGS): https://lpdaac.usgs.gov/products/gfsad30saafgircev001/

GIScience and Remote Sensing

Regional protocol framework for the inventory and monitoring of breeding Atlantic Coast Piping Plovers

This regional protocol provides a framework for quantifying the number of breeding pairs and productivity of Atlantic Coast piping plover (Charadrius melodus) populations during the breeding season. A primary purpose of this protocol is to standardize piping plover monitoring during the breeding season. The survey techniques described herein involve repeated visual counts of adults, nests, eggs, and chicks within a defined survey site (i.e., beach) as well as visual identification of potential threats to survival and productivity. Resulting data can be compiled and analyzed across multiple geographic units (i.e., sites, states, and recovery units) to assess progress toward recovery goals, inform local management decisions, assess management effectiveness, and improve monitoring efforts. This protocol framework was developed as part of the United States Fish and Wildlife Service (USFWS) National Wildlife Refuge System (NWRS) Inventory and Monitoring (I&M) Initiative in coordination with Ecological Services (ES) and state coordinators within the Southeast and Northeast Regions (4 and 5, respectively). Although this protocol framework is to be used primarily by NWRS to inform recovery goals, assist with local management decision-making, and meet State reporting requirements, the approach strives to assist monitoring efforts of nonNWRS partners, such as other federal agencies (e.g. National Park Service), State wildlife agencies, non-governmental organizations, and private landowners. This protocol framework and associated data management system (PIPLweb) aims to interface with existing data management and analysis tools (i.e., PIPLODES, NestStory, and PiperEx) to ensure that data collection is efficient and comparable across scales, and supports management decisions across partners. The content and structure of the protocol framework follows standards set forth in the USFWS’s How to Develop Survey Protocols: A Handbook (Version 1.0; 2013). The eight elements addressed include: introduction, sampling design, field methods, data management and analysis, reporting, personnel requirements and training, operational requirements, and references. A series of standard operating procedures (SOPs) provides greater detail on recommended methods and technical aspects of this protocol. Data entry, archival, and multi-scale analysis are handled through a secure web application (Plover Inventory and Productivity Library; PIPLweb) developed by the United States Geological Survey (USGS). When management activities and survey objectives are similar across management units, partners (Refuges, other federal agencies, State, NGOs, private) are encouraged to use this protocol framework to develop stepped-down site-specific survey protocols that include guidance for conducting on-the-ground monitoring and management plans.

Regional Protocol Framework

Using biological responses to monitor freshwater post-spill conditions over 3 years in Blacktail Creek, North Dakota, USA

A pipeline carrying unconventional oil and gas (OG) wastewater spilled approximately 11 million liters of wastewater into Blacktail Creek, North Dakota, USA. Flow of the mix of stream water and wastewater down the channel resulted in storage of contaminants in the hyporheic zone and along the banks, providing a long-term source of wastewater constituents to the stream. A multi-level investigation was used to assess the potential effects of oil and brine spills on aquatic life. In this study, we used a combination of experiments using a native fish species, Fathead Minnow ( Pimephales promelas ), field sampling of the microbial community structure, and measures of estrogenicity. The fish investigation included in situ experiments and experiments with collected site water. Estrogenicity was measured in collected site water samples, and microbial community analyses were conducted on collected sediments. During the initial post-spill investigation, February 2015, performing in situ fish bioassays was impossible because of ice conditions. However, microbial community (e.g., the presence of members of the Halomonadaceae, a family that is indicative of elevated salinity) and estrogenicity differences were compared to reference sites and point to early biological effects of the spill. We noted water column effects on in situ fish survival 6 months post-spill during June 2015. At that time, total dissolved ammonium (sum of ammonium and ammonia, TAN) was 4.41 mg NH 4 /L with an associated NH 3 of 1.09 mg/L, a concentration greater than the water quality criteria established to protect aquatic life. Biological measurements in the sediment defined early and long-lasting effects of the spill on aquatic resources. The microbial community structure was affected during all sampling events. Therefore, sediment may act as a sink for constituents spilled and as such provide an indication of continued and cumulative effects post-spill. However, lack of later water column effects may reflect pulse hyporheic flow of ammonia from shallow ground water. Combining fish toxicological, microbial community structure and estrogenicity information provides a complete ecological investigation that defines potential influences of contaminants at organismal, population, and community levels. In general, in situ bioassays have implications for the individual survival and changes at the population level, microbial community structure defines potential changes at the community level, and estrogenicity measurements define changes at the individual and molecular level. By understanding effects at these various levels of biological organization, natural resource managers can interpret how a course of action, especially for remediation/restoration, might affect a larger group of organisms in the system. The current work also reviews potential effects of additional constituents defined during chemistry investigations on aquatic resources.

North Dakota

Fish assemblages of the Casiquiare River, a corridor and zoogeographical filter for dispersal between the Orinoco and Amazon basins

Aim: The aim of this study was to determine whether the Casiquiare River functions as a free dispersal corridor or as a partial barrier (i.e. filter) for the interchange of fish species of the Orinoco and Negro/Amazon basins using species assemblage patterns according to geographical location and environmental features. Location: The Casiquiare, Upper Orinoco and Upper Negro rivers in southern Venezuela, South America. Methods: Our study was based on an analysis of species presence/absence data and environmental information (11 habitat characteristics) collected by the authors and colleagues between the years 1984 and 1999. The data set consisted of 269 sampled sites and 452 fish species (> 50,000 specimens). A wide range of habitat types was included in the samples, and the collection sites were located at various points along the entire length of the Casiquiare main channel, at multiple sites on its tributary streams, as well as at various nearby sites outside the Casiquiare drainage, within the Upper Orinoco and Upper Rio Negro river systems. Most specimens and field data used in this analysis are archived in the Museo de Ciencias Naturales in Guanare, Venezuela. We performed canonical correspondence analysis (CCA) based on species presence/absence using two versions of the data set: one that eliminated sites having < 5 species and species occurring at < 5 sites; and another that eliminated sites having < 10 species and species occurring at < 10 sites. Cluster analysis was performed on sites based on species assemblage similarity, and a separate analysis was performed on species based on CCA loadings. Results: The CCA results for the two versions of the data set were qualitatively the same. The dominant environmental axis contrasted assemblages and sites associated with blackwater vs. clearwater conditions. Longitudinal position on the Casiquiare River was correlated (r2 = 0.33) with CCA axis-1 scores, reflecting clearwater conditions nearer to its origin (bifurcation of the Orinoco) and blackwater conditions nearer to its mouth (junction with the Rio Negro). The second CCA axis was most strongly associated with habitat size and structural complexity. Species associations derived from the unweighted pair-group average clustering method and pair-wise squared Euclidean distances calculated from species loadings on CCA axes 1 and 2 showed seven ecological groupings. Cluster analysis of species assemblages according to watershed revealed a stronger influence of local environmental conditions than of geographical proximity. Main conclusions: Fish assemblage composition is more consistently associated with local environmental conditions than with geographical position within the river drainages. Nonetheless, the results support the hypothesis that the mainstem Casiquiare represents a hydrochemical gradient between clearwaters at its origin and blackwaters at its mouth, and as such appears to function as a semi-permeable barrier (environmental filter) to dispersal and faunal exchanges between the partially vicariant fish faunas of the Upper Orinoco and Upper Negro rivers. ?? 2008 The Authors.

Journal of Biogeography

Analysis of seafloor change around Dauphin Island, Alabama, 1987–2015

Dauphin Island is a 26-km-long barrier island located southwest of Mobile Bay, Alabama, in the north-central Gulf of Mexico. The island contains sandy beaches, dunes, maritime forests, freshwater ponds and intertidal wetlands, providing habitat for many endangered and threatened species. Dauphin Island also provides protection for and maintains estuarine conditions within Mississippi Sound, supporting oyster habitat and seagrasses. Wetland marshes along the Alabama mainland are protected by the island from wave-induced erosion during storms approaching from the Gulf of Mexico. Over the years, the island has been eroded by storms, most recently by Hurricane Ivan (2004) and Hurricane Katrina (2005) (Ivan/Katrina), which breached the island along its narrowest extent and caused damage to infrastructure. Along with storms producing significant episodic change, long-term beach erosion has exposed numerous pine tree stumps in the shoreface. The stumps are remnants of past maritime forests and reflect the consistent landward retreat of the island. Island change has prompted the State of Alabama to evaluate restoration alternatives to increase island resilience and sustainability by protecting and preserving the natural habitat, and by understanding the processes that influence shoreline change. Under a grant from the National Fish and Wildlife Foundation, restoration alternatives are being developed that will allow the State to make decisions on engineering and ecological restoration designs based on scientific analysis of likely outcomes and tradeoffs between impacts to stakeholder interests. Science-based assessment of the coastal zone requires accurate and up-to-date baseline data to provide a valid image of present conditions and to support modeling of coastal processes. Bathymetric elevation measurements are essential to this requirement. In August 2015, the U.S. Army Corps of Engineers and the U.S. Geological Survey conducted single beam and multibeam bathymetric surveys around Dauphin Island using a variety of shallow draft vessels and equipment. More than 95 square kilometers of seafloor was imaged. The data were integrated into a seamless digital elevation model to provide a high-resolution bathymetric map of the seafloor extending 9.5 kilometers seaward from the island’s eastern end and approximately 2 km along the rest of the island on the gulf and sound sides. Water depths range from 0.3 to 15.0 meters (m), with depths greater than 10.0 m constrained to the Mobile ship channel on the extreme eastern flank of the coverage. To measure seafloor change, two periods of historic hydrographic survey data were acquired from the National Oceanic and Atmospheric Administration National Centers for Environmental Information data archive. The two timeframes (1987–1988 and 2005–2007) were selected for their completeness of spatial coverage and because they encompass a period of significant storm impacts to the island. These timeframes were compared to each other and with the 2015 dataset to monitor elevation gain (sediment accretion) and elevation loss (sediment erosion) over time. Sediment dynamics is by far the most significant driver of nearshore elevation change in this area. The Mississippi-Alabama inner shelf is a passive margin, and other influences on elevation change (for example, tectonic adjustment, Holocene subsidence, and eustatic sea-level rise) are neither significant nor variable enough over this time period to have an imprint.

Alabama

Occurrence of a suite of stream-obligate amphibians in timberlands of Mendocino County, California, examined using environmental DNA

Stream-obligate amphibians are important indicators of ecosystem health in the Pacific Northwest, but distributional information to improve forest management is lacking in many regions. We analyzed archived DNA extracted from water samples in 60 pools in streams on private timberlands in Mendocino County, California, for 3 California Species of Special Concern—Coastal Tailed Frogs ( Ascaphus truei ), Foothill Yellow-legged Frogs ( Rana boylii ), and Southern Torrent Salamanders ( Rhyacotriton variegatus )—to better understand their distributions in the region. Detection probabilities for eDNA of Foothill Yellow-legged Frogs and Coastal Tailed Frogs were positively influenced by water temperature. eDNA occurrence for both frogs was affected by whether silt or organic matter was a dominant substrate in the sampled pool, and Foothill Yellow-legged Frog eDNA occurrence was also affected by water temperature. Foothill Yellow-legged Frog eDNA occurrence had a strong, positive association with water temperature, with occurrence unlikely below 14°C and very likely above 16°C, and a positive association with silt or organic substrates in pools, which was likely an indicator of higher-order stream reaches. In contrast, Coastal Tailed Frogs had a negative association with silt or organic substrates. Historical visual detections were generally congruent with findings using eDNA, but differences highlight important areas for further study. We did not detect Southern Torrent Salamanders using eDNA at any sites. Our study reinforces that ecological relationships of these species are varied, and shows the importance of maintaining the integrity of streams with diverse characteristics for conserving stream amphibians.

California

Proceedings of the first U.S. Geological Survey scientific information management workshop, March 21-23, 2006

In March 2006, the U.S. Geological Survey (USGS) held the first Scientific Information Management (SIM) Workshop in Reston, Virginia. The workshop brought together more than 150 SIM professionals from across the organization to discuss the range and importance of SIM problems, identify common challenges and solutions, and investigate the use and value of “communities of practice” (CoP) as mechanisms to address these issues. The 3-day workshop began with presentations of SIM challenges faced by the Long Term Ecological Research (LTER) network and two USGS programs from geology and hydrology. These presentations were followed by a keynote address and discussion of CoP by Dr. Etienne Wenger, a pioneer and leading expert in CoP, who defined them as "groups of people who share a passion for something that they know how to do and who interact regularly to learn how to do it better." Wenger addressed the roles and characteristics of CoP, how they complement formal organizational structures, and how they can be fostered. Following this motivating overview, five panelists (including Dr. Wenger) with CoP experience in different institutional settings provided their perspectives and lessons learned. The first day closed with an open discussion on the potential intersection of SIM at the USGS with SIM challenges and the potential for CoP. The second session began the process of developing a common vocabulary for both scientific data management and CoP, and a list of eight guiding principles for information management were proposed for discussion and constructive criticism. Following this discussion, 20 live demonstrations and posters of SIM tools developed by various USGS programs and projects were presented. Two community-building sessions were held to explore the next steps in 12 specific areas: Archiving of Scientific Data and Information; Database Networks; Digital Libraries; Emerging Workforce; Field Data for Small Research Projects; Knowledge Capture; Knowledge Organization Systems and Controlled Vocabularies; Large Time Series Data Sets; Metadata; Portals and Frameworks; Preservation of Physical Collections; and Scientific Data from Monitoring Programs. In about two-thirds of these areas, initial steps to forming CoP are now underway. The final afternoon included a panel in which information professionals, managers, program coordinators, and associate directors shared their perspectives on the workshop, on ways in which the USGS could better manage its scientific information, and on the use of CoP as informal mechanisms to complement formal organizational structures. The final session focused on developing the next steps, an action plan, and a communication strategy to ensure continued development.

Scientific Investigations Report

Preprint: Simulated seasonal loads of total nitrogen and total phosphorus by major source from watersheds draining to Washington waters of the Salish Sea, 2005 through 2020

The U.S. Geological Survey (USGS) and the Washington State Department of Ecology (Ecology) have developed watershed models of seasonal load estimates of total nitrogen (TN) and total phosphorus (TP) discharging into the Washington waters of the Salish Sea from 2005 through 2020. The modeling approach used was dynamic SPARROW (SPAtially Referenced Regressions On Watershed attributes), a statistical-physical watershed modeling technique, initially applied at large spatial scales to represent long-term average stream loads throughout a stream network, refined here to estimate seasonal TN and TP loads across watersheds to clarify upstream contributions from discernable point and nonpoint sources delivered to marine waters at surface water confluences along the shoreline and quantify when, where, and why they were high or low. Upstream contributing sources included permitted treated wastewater facilities, crop fertilizer, animal feeding operations, septic systems, urban land and stormwater, atmospheric deposition (TN only), nitrogen fixation by Red Alder Alnus rubra trees (TN only), and background geologic material (TP only). Instream load magnitudes and their source compositions varied widely across watersheds, and even within each watershed, yet the largest loads typically occurred in the large rivers during winter and fall when streamflow was highest. Likewise, instream loads were typically lowest in summer during low streamflow, yet the relative instream aquatic decay was highest. The seasonal storage lag component of those nonpoint sources was estimated to contribute a quarter of the seasonal instream load during winter and fall high streamflow and sometimes half of the instream load during summer low streamflow. A key aspect of Ecology’s current Puget Sound Nutrient Source Reduction Project is consideration of upstream watershed contributions of nutrients to their marine-water discharge points. Simulated seasonal loads carried by streams to 63 river mouth marine discharge points 9 ranged by several orders-of-magnitude for both TN and TP due to the spatial and seasonal differences in hydrologic flows, magnitude and timing of contributing sources, and in-stream decay. The Snohomish and Skagit Rivers discharged the largest TN and TP loads, yet the Samish River was shown to have some of the highest TN and TP yields and concentrations. Additionally, a reference scenario was developed to provide an estimate of the pre-industrial local and regional loads.

ESS Open Archive

Baseline ecological risk assessment of the Calcasieu Estuary, Louisiana: Part 2. An evaluation of the predictive ability of effects-based sediment-quality guidelines

Three sets of effects-based sediment-quality guidelines (SQGs) were evaluated to support the selection of sediment-quality benchmarks for assessing risks to benthic invertebrates in the Calcasieu Estuary, Louisiana. These SQGs included probable effect concentrations (PECs), effects range median values (ERMs), and logistic regression model (LRMs)-based T 50 values. The results of this investigation indicate that all three sets of SQGs tend to underestimate sediment toxicity in the Calcasieu Estuary (i.e., relative to the national data sets), as evaluated using the results of 10-day toxicity tests with the amphipod, Hyalella azteca , or Ampelisca abdita , and 28-day whole-sediment toxicity tests with the H. azteca . These results emphasize the importance of deriving site-specific toxicity thresholds for assessing risks to benthic invertebrates.

Louisiana

Development of working hypotheses linking management of the Missouri River to population dynamics of Scaphirhynchus albus (pallid sturgeon)

This report documents a process of filtering of hypotheses that relate Missouri River Scaphirhynchus albus (pallid sturgeon) population dynamics to management actions including flow alterations, channel reconfigurations, and pallid sturgeon population augmentation. The filtering process was a partnership among U.S. Geological Survey, U.S. Army Corps of Engineers, and U.S. Fish and Wildlife Service to contribute to the Missouri River Recovery Management Plan process. The objective of the filtering process was to produce a set of hypotheses with high relevance to pallid sturgeon population dynamics and decision making on the Missouri River. The Missouri River Pallid Sturgeon Effects Analysis team filtered hundreds of potential hypotheses implicit in conceptual ecological models to develop a set of 40 candidate dominant hypotheses that were identified by experts as being important in pallid sturgeon population dynamics. Using a modified Delphi process and additional expert opinion, the team reduced this set of hypotheses to 23 working dominant hypotheses. We then matched the 23 hypotheses with management actions that could influence the biotic outcomes, resulting in as many as 176 potential effects between management actions and pallid sturgeon in the Missouri River. This number was consolidated to a candidate set of 53 working management hypotheses because some management actions applied to multiple life stages of the pallid sturgeon. We used an additional round of expert surveys to identify a set of 30 working management hypotheses. Finally, the set of working management hypotheses was filtered by the U.S. Army Corps of Engineers, Missouri River Recovery Program for actions that were within the agency&rsquo;s authority and jurisdiction. This round resulted in a set of 21 hypotheses for initial modeling of linkages from management to pallid sturgeon population responses. The initial set of candidate hypotheses provides a useful starting point for quantitative modeling and adaptive management of the river and species. We anticipate that hypotheses will change from the set of working management hypotheses as adaptive management progresses. More importantly, hypotheses that have been filtered out of our multistep process are still being considered. These filtered hypotheses are archived and if existing hypotheses are determined to be inadequate to explain observed population dynamics, new hypotheses can be created or filtered hypotheses can be reinstated.

Missouri River Basin

Organization of marine phenology data in support of planning and conservation in ocean and coastal ecosystems

Among the many effects of climate change is its influence on the phenology of biota. In marine and coastal ecosystems, phenological shifts have been documented for multiple life forms; however, biological data related to marine species' phenology remain difficult to access and is under-used. We conducted an assessment of potential sources of biological data for marine species and their availability for use in phenological analyses and assessments. Our evaluations showed that data potentially related to understanding marine species' phenology are available through online resources of governmental, academic, and non-governmental organizations, but appropriate datasets are often difficult to discover and access, presenting opportunities for scientific infrastructure improvement. The developing Federal Marine Data Architecture when fully implemented will improve data flow and standardization for marine data within major federal repositories and provide an archival repository for collaborating academic and public data contributors. Another opportunity, largely untapped, is the engagement of citizen scientists in standardized collection of marine phenology data and contribution of these data to established data flows. Use of metadata with marine phenology related keywords could improve discovery and access to appropriate datasets. When data originators choose to self-publish, publication of research datasets with a digital object identifier, linked to metadata, will also improve subsequent discovery and access. Phenological changes in the marine environment will affect human economics, food systems, and recreation. No one source of data will be sufficient to understand these changes. The collective attention of marine data collectors is needed—whether with an agency, an educational institution, or a citizen scientist group—toward adopting the data management processes and standards needed to ensure availability of sufficient and useable marine data to understand marine phenology.

Ecological Informatics

Effects of geolocation archival tags on reproduction and adult body mass of sooty shearwaters (Puffinus griseus)

We attached 11 g (1.4% body-mass equivalent) global location sensing (GLS) archival tag packages to tarsi of 25 breeding sooty shearwaters (Puffinus griseus, titi) on Whenua Hou (Codfish Island), New Zealand during the chick-rearing period in 2005. Compared with chicks reared by non-handled adults that did not carry tags, deployment of tags on one or both adult parents ultimately resulted in 35% reduction in chick body mass and significantly reduced chick skeletal size preceding fledging (19 April). However, body mass between chick groups was not significantly different after controlling for skeletal size. Effects on chicks were more pronounced in six pairs where both parents carried tags. Chick mass was negatively related to the duration that adults carried tags. In this study, none of the chicks reared by pairs where both parents were tagged, 54% of chicks reared by pairs where one parent was tagged, and 83% of chicks reared by non-handled and non-tagged parents achieved a previously determined pre-fledging mass threshold (564 g; Sagar & Horning 1998). Body mass of adults carrying tags and returning from transequatorial migration the following year were 4% lighter on average than non-tagged birds, but this difference was not statistically significant. Reduced mass among chicks reared by adults carrying tags during the chick-provisioning period indicated that adults altered "normal" provisioning behaviours to maintain their own body condition at the expense of their chicks. Population-level information derived from telemetry studies can reveal important habitat-linked behaviours, unique aspects of seabird foraging behaviours, and migration ecology. Information for some species (e.g., overlap with fisheries) can aid conservation and marine ecosystem management. We advise caution, however, when interpreting certain data related to adult provisioning behaviours (e.g., time spent foraging, provisioning rates, etc.). If effects on individuals are of concern, we suggest shorter-term deployments, smaller and lighter tags, and alternative attachment techniques, especially when investigating threatened or endangered species. ?? The Royal Society of New Zealand 2009.

New Zealand Journal of Ecology

VEMAP Phase 2 bioclimatic database. I. Gridded historical (20th century) climate for modeling ecosystem dynamics across the conterminous USA

Analysis and simulation of biospheric responses to historical forcing require surface climate data that capture those aspects of climate that control ecological processes, including key spatial gradients and modes of temporal variability. We developed a multivariate, gridded historical climate dataset for the conterminous USA as a common input database for the Vegetation/Ecosystem Modeling and Analysis Project (VEMAP), a biogeochemical and dynamic vegetation model intercomparison. The dataset covers the period 1895—1993 on a 0.5° latitude/longitude grid. Climate is represented at both monthly and daily timesteps. Variables are: precipitation, mininimum and maximum temperature, total incident solar radiation, daylight-period irradiance, vapor pressure, and daylight-period relative humidity. The dataset was derived from US Historical Climate Network (HCN), cooperative network, and snowpack telemetry (SNOTEL) monthly precipitation and mean minimum and maximum temperature station data. We employed techniques that rely on geostatistical and physical relationships to create the temporally and spatially complete dataset. We developed a local kriging prediction model to infill discontinuous and limited-length station records based on spatial autocorrelation structure of climate anomalies. A spatial interpolation model (PRISM) that accounts for physiographic controls was used to grid the infilled monthly station data. We implemented a stochastic weather generator (modified WGEN) to disaggregate the gridded monthly series to dailies. Radiation and humidity variables were estimated from the dailies using a physically-based empirical surface climate model (MTCLIM3). Derived datasets include a 100 yr model spin-up climate and a historical Palmer Drought Severity Index (PDSI) dataset. The VEMAP dataset exhibits statistically significant trends in temperature, precipitation, solar radiation, vapor pressure, and PDSI for US National Assessment regions. The historical climate and companion datasets are available online at data archive centers.

Climate Research

Behavioural thermoregulation and bioenergetics of riverine smallmouth bass associated with ambient cold-period thermal refuge

Smallmouth bass in thermally heterogeneous streams may behaviourally thermoregulate during the cold period (i.e., groundwater temperature greater than river water temperature) by inhabiting warm areas in the stream that result from high groundwater influence or springs. Our objectives were to determine movement of smallmouth bass ( Micropterus dolomieu ) that use thermal refuge and project differences in growth and consumption among smallmouth bass exhibiting different thermal-use patterns. We implanted radio transmitters in 29 smallmouth bass captured in Alley Spring on the Jacks Fork River, Missouri, USA, during the winter of 2012. Additionally, temperature archival tags were implanted in a subset of nine fish. Fish were tracked using radio telemetry monthly from January 2012 through January of 2013. The greatest upstream movement was 42.5 km, and the greatest downstream movement was 22.2 km. Most radio tagged fish (69%) departed Alley Spring when daily maximum river water temperature first exceeded that of the spring (14 &deg;C) and during increased river discharge. Bioenergetic modelling predicted that a 350 g migrating smallmouth bass that used cold-period thermal refuge would grow 16% slower at the same consumption level as a fish that did not seek thermal refuge. Contrary to the bioenergetics models, extrapolation of growth scope results suggested migrating fish grow 29% more than fish using areas of stream with little groundwater influence. Our results contradict previous findings that smallmouth bass are relatively sedentary, provide information about potential cues for migratory behaviour, and give insight to managers regarding use and growth of smallmouth bass in thermally heterogeneous river systems.

Missouri

Age and sex specific timing, frequency, and spatial distribution of horseshoe crab spawning in Delaware Bay: Insights from a large-scale radio telemetry array

To study horseshoe crab Limulus polyphemus spawning behavior and migration over a large-spatial extent (>100 km), we arrayed fixed station radio receivers throughout Delaware Bay and deployed radio transmitters and archival tags on adult horseshoe crabs prior to their spawning season. We tagged and released 160 females and 60 males in 2004 and 217 females in 2005. The array covered approximately 140 km of shoreline. Recapture rates were >70% with multi-year recaptures. We categorized adult age by carapace wear. Older females tended to spawn earlier in the season and more frequently than young females, but those tendencies were more apparent in 2004 when spawning overall occurred earlier than in 2005 when spawning was delayed possibly due to decreased water temperatures. Timing of initial spawning within a year was correlated with water temperature. After adjusting for day of first spring tide, the day of first spawning was 4 days earlier for every 1 degree (̊C) rise in mean daily water temperature in May. Seventy nine % of spawning occurred during nighttime high tides. Fifty five % of spawning occurred within 3 d of a spring tide, which was slightly higher than the 47% expected if spawning was uniformly distributed regardless of tidal cycle. Within the same spawning season, males and females were observed spawning or intertidally resting at more than one beach separated by >5 km. Between years, most (77%) did not return to spawn at the same beach. Probability of stranding was strongly age dependent for males and females with older adults experiencing higher stranding rates. Horseshoe crabs staging in the shallow waters east of the channel spawned exclusively along the eastern (NJ) shoreline, but those staging west of the channel spawned throughout the bay. Overall, several insights emerged from the use of radio telemetry, which advances our understanding of horseshoe crab ecology and will be useful in conserving the Delaware Bay horseshoe crab population and habitats.

Delaware, New Jersey

Biomedical health assessments of the Florida manatee in Crystal River - providing opportunities for training during the capture, handling, and processing of this endangered aquatic mammal

Federal and state researchers have been involved in manatee (Trichechus manatus) biomedical health assessment programs for a couple of decades. These benchmark studies have provided a foundation for the development of consistent capture, handling, and processing techniques and protocols. Biologists have implemented training and encouraged multi-agency participation whenever possible to ensure reliable data acquisition, recording, sample collection, publication integrity, and meeting rigorous archival standards. Under a U.S. Fish and Wildlife Service wildlife research permit granted to the U.S. Geological Survey (USGS) Sirenia Project, federal biologists and collaborators are allowed to conduct research studies on wild and captive manatees detailing various aspects of their biology. Therefore, researchers with the project have been collaborating on numerous studies over the last several years. One extensive study, initiated in 2006 has focused on health and fitness of the winter manatee population located in Crystal River, Florida. During those health assessments, capture, handling, and work-up training has been afforded to many of the participants. That study has successfully captured and handled 123 manatees. The data gathered have provided baseline information on manatee health, reproductive status, and nutritional condition. This research initiative addresses concerns and priorities outlined in the Florida Manatee Recovery Plan. The assessment teams strive to continue this collaborative effort to help advance our understanding of health-related issues confronting manatees throughout their range and interlacing these findings with surrogate species concepts.

Florida

When a habitat freezes solid: Microorganisms over-winter within the ice column of a coastal Antarctic lake

A major impediment to understanding the biology of microorganisms inhabiting Antarctic environments is the logistical constraint of conducting field work primarily during the summer season. However, organisms that persist throughout the year encounter severe environmental changes between seasons. In an attempt to bridge this gap, we collected ice core samples from Pony Lake in early November 2004 when the lake was frozen solid to its base, providing an archive for the biological and chemical processes that occurred during winter freezeup. The ice contained bacteria and virus-like particles, while flagellated algae and ciliates over-wintered in the form of inactive cysts and spores. Both bacteria and algae were metabolically active in the ice core melt water. Bacterial production ranged from 1.8 to 37.9 μg C L −1 day −1 . Upon encountering favorable growth conditions in the melt water, primary production ranged from 51 to 931 μg C L −1 day −1 . Because of the strong H 2 S odor and the presence of closely related anaerobic organisms assigned to Pony Lake bacterial 16S rRNA gene clones, we hypothesize that the microbial assemblage was strongly affected by oxygen gradients, which ultimately restricted the majority of phylotypes to distinct strata within the ice column. This study provides evidence that the microbial community over-winters in the ice column of Pony Lake and returns to a highly active metabolic state when spring melt is initiated.

FEMS Microbiology Ecology

A 30-m landsat-derived cropland extent product of Australia and China using random forest machine learning algorithm on Google Earth Engine cloud computing platform

Mapping high resolution (30-m or better) cropland extent over very large areas such as continents or large countries or regions accurately, precisely, repeatedly, and rapidly is of great importance for addressing the global food and water security challenges. Such cropland extent products capture individual farm fields, small or large, and are crucial for developing accurate higher-level cropland products such as cropping intensities, crop types, crop watering methods (irrigated or rainfed), crop productivity, and crop water productivity. It also brings many challenges that include handling massively large data volumes, computing power, and collecting resource intensive reference training and validation data over complex geographic and political boundaries. Thereby, this study developed a precise and accurate Landsat 30-m derived cropland extent product for two very important, distinct, diverse, and large countries: Australia and China. The study used of eight bands (blue, green, red, NIR, SWIR1, SWIR2, TIR1, and NDVI) of Landsat-8 every 16-day Operational Land Imager (OLI) data for the years 2013–2015. The classification was performed by using a pixel-based supervised random forest (RF) machine learning algorithm (MLA) executed on the Google Earth Engine (GEE) cloud computing platform. Each band was time-composited over 4–6 time-periods over a year using median value for various agro-ecological zones (AEZs) of Australia and China. This resulted in a 32–48-layer mega-file data-cube (MFDC) for each of the AEZs. Reference training and validation data were gathered from: (a) field visits, (b) sub-meter to 5-m very high spatial resolution imagery (VHRI) data, and (c) ancillary sources such as from the National agriculture bureaus. Croplands versus non-croplands knowledge base for training the RF algorithm were derived from MFDC using 958 reference-training samples for Australia and 2130 reference-training samples for China. The resulting 30-m cropland extent product was assessed for accuracies using independent validation samples: 900 for Australia and 1972 for China. The 30-m cropland extent product of Australia showed an overall accuracy of 97.6% with a producer’s accuracy of 98.8% (errors of omissions = 1.2%), and user’s accuracy of 79% (errors of commissions = 21%) for the cropland class. For China, overall accuracies were 94% with a producer’s accuracy of 80% (errors of omissions = 20%), and user’s accuracy of 84.2% (errors of commissions = 15.8%) for cropland class. Total cropland areas of Australia were estimated as 35.1 million hectares and 165.2 million hectares for China. These estimates were higher by 8.6% for Australia and 3.9% for China when compared with the traditionally derived national statistics. The cropland extent product further demonstrated the ability to estimate sub-national cropland areas accurately by providing an R 2 value of 0.85 when compared with province-wise cropland areas of China. The study provides a paradigm-shift on how cropland maps are produced using multi-date remote sensing. These products can be browsed at www.croplands.org and made available for download at NASA’s Land Processes Distributed Active Archive Center (LP DAAC) https://www.lpdaac.usgs.gov/node/1282 .

ISPRS Journal of Photogrammetry and Remote Sensing