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At least 847 records · Page 47Linked to original sources

Effects of methyl testosterone exposure on sexual differentiation in medaka, Oryzias latipes

Studies were conducted to characterize effects of a known androgen on sexual differentiation and development of medaka, Oryzias latipes (d-rR strain), at two life stages. Embryos were injected with graded doses of methyl testosterone (MT) prior to epiboly. The occurrence of sex-reversal, and the gonadosomatic index (GSI) were evaluated in adults. Primary germ cells were counted and gonad volumes calculated for larvae to determine if sex-reversal could be detected at an early life stage. Sex-reversal of genetic females to phenotypic males was observed at both life stages. The GSI for phenotypic females was greater than for phenotypic males, while the GSI in XX males was similar to XY males. MT appeared to reduce the GSI of XX females exposed to MT but not sex-reversed. Our results indicate that embryonic exposure to androgens influences sexual development in medaka. Utilizing the d-rR strain of medaka allows detection of an effect as early as 2 weeks after chemical exposure making this a useful tool to screen chemicals for effects on sexual differentiation. Copyright (C) 2000.

Conference Paper↗

The oral bioavailability and toxicokinetics of methylmercury in common loon ( Gavia immer ) chicks

We compared the toxicokinetics of methylmercury in captive common loon chicks during two time intervals to assess the impact of feather growth on the kinetics of mercury. We also determined the oral bioavailability of methylmercury during these trials to test for age-related changes. The blood concentration-time curves for individuals dosed during feather development (initiated 35 days post hatch) were best described by a one-compartment toxicokinetic model with an elimination half-life of 3 days. The data for birds dosed following completion of feather growth (84 days post hatch) were best fitted by a two-compartment elimination model that includes an initial rapid distribution phase with a half-life of 0.9 days, followed by a slow elimination phase with a half-life of 116 days. We determined the oral bioavailability of methylmercury during the first dosing interval by comparing the ratios of the area under the blood concentration-time curves (AUC 0→∞ ) for orally and intravenously dosed chicks. The oral bioavailability of methylmercury during the first dosing period was 0.83. We also determined bioavailability during both dosing periods using a second measure because of irregularities with intravenous results in the second period. This second bioavailability measure estimated the percentage of the dose that was deposited in the blood volume (f), and the results show that there was no difference in bioavailability among dosing periods. The results of this study highlight the importance of feather growth on the toxicokinetics of methylmercury.

Comparative Biochemistry and Physiology, Part A: M↗

Global volcanic hazards and risk

An estimated 800 million people live within 100 km of an active volcano in 86 countries and additional overseas territories worldwide [see Chapter 4 and Appendix B]1. Volcanoes are compelling evidence that the Earth is a dynamic planet characterised by endless change and renewal. Humans have always found volcanic activity fascinating and have often chosen to live close to volcanoes, which commonly provide favourable environments for life. Volcanoes bring many benefits to society: eruptions fertilise soils; elevated topography provides good sites for infrastructure (e.g. telecommunications on elevated ground); water resources are commonly plentiful; volcano tourism can be lucrative; and volcanoes can acquire spiritual, aesthetic or religious significance. Some volcanoes are also associated with geothermal resources, making them a target for exploration and a potential energy resource. Much of the time volcanoes are not a threat because they erupt very infrequently or because communities have become resilient to frequently erupting volcanoes. However, there is an everpresent danger of a long-dormant volcano re-awakening or of volcanoes producing anomalously large or unexpected eruptions. Volcanic eruptions can cause loss of life and livelihoods in exposed communities, damage or disrupt critical infrastructure and add stress to already fragile environments. Their impacts can be both short-term, e.g. physical damage, and long-term, e.g. sustained or permanent displacement of populations. The risk from volcanic eruptions and their attendant hazards is often underestimated beyond areas within the immediate proximity of a volcano. For example, volcanic ash hazards can have effects hundreds of kilometres away from the vent and have an adverse impact on human and animal health, infrastructure, transport, agriculture and horticulture, the environment and economies. The products of volcanism and their impacts can extend beyond country borders, to be regional and even global in scale. Although known historical loss of life from volcanic eruptions (since 1600 AD about 280,000 fatalities are recorded, Auker et al. (2013)) is modest compared to other major natural hazards, volcanic eruptions can be catastrophic for exposed communities. In 1985 the town of Armero in Colombia was buried by lahars (volcanic mudflows) with more than 21,000 fatalities due to relatively small explosive eruptions at the summit of Nevado del Ruiz volcano that partially melted a glacier (Voight, 1990).

Book chapter↗

Emergence flux declines disproportionately to larval density along a stream metals gradient

Effects of contaminants on adult aquatic insect emergence are less well understood than effects on insect larvae. We compared responses of larval density and adult emergence along a metal contamination gradient. Nonlinear threshold responses were generally observed for larvae and emergers. Larval densities decreased significantly at low metal concentrations but precipitously at concentrations of metal mixtures above aquatic life criteria (Cumulative Criterion Accumulation Ratio (CCAR) ≥ 1). In contrast, adult emergence declined precipitously at low metal concentrations (CCAR ≤ 1), followed by a modest decline above this threshold. Adult emergence was a more sensitive indicator of the effect of low metals concentrations on aquatic insect communities compared to larvae, presumably because emergence is limited by a combination of larval survival and other factors limiting successful emergence. Thus effects of exposure to larvae are not manifest until later in life (during metamorphosis and emergence). This loss in emergence reduces prey subsidies to riparian communities at concentrations considered safe for aquatic life. Our results also challenge the widely held assumption that adult emergence is a constant proportion of larval densities in all streams.

Environmental Science & Technology↗

Pesticides in U.S. streams and rivers: occurrence and trends during 1992-2011

During the 20 years from 1992 to 2011, pesticides were found at concentrations that exceeded aquatic-life benchmarks in many rivers and streams that drain agricultural, urban, and mixed-land use watersheds. Overall, the proportions of assessed streams with one or more pesticides that exceeded an aquatic-life benchmark were very similar between the two decades for agricultural (69% during 1992−2001 compared to 61% during 2002−2011) and mixed-land-use streams (45% compared to 46%). Urban streams, in contrast, increased from 53% during 1992−2011 to 90% during 2002−2011, largely because of fipronil and dichlorvos. The potential for adverse effects on aquatic life is likely greater than these results indicate because potentially important pesticide compounds were not included in the assessment. Human-health benchmarks were much less frequently exceeded, and during 2002−2011, only one agricultural stream and no urban or mixed-land-use streams exceeded human-health benchmarks for any of the measured pesticides. Widespread trends in pesticide concentrations, some downward and some upward, occurred in response to shifts in use patterns primarily driven by regulatory changes and introductions of new pesticides.

Environmental Science & Technology↗

Ontogenetic behavior, migration, and social behavior of pallid sturgeon, Scaphirhynchus albus, and shovelnose sturgeon, S. platorynchus, with notes on the adaptive significance of body color

We conducted laboratory studies on the ontogenetic behavior of free embryos (first life interval after hatching) and larvae (first feeding interval) of pallid and shovelnose sturgeon. Migration styles of both species were similar for timing of migration (initiation by embryos on day 0 after hatching and cessation by larvae on days 12-13 at 236-243 cumulative temperature degree units), migration distance (about 13 km), life interval when most distance was moved (embryo), and diel behavior of embryos (diurnal). However, the species differed for two behaviors: movement characteristics of embryos (peak movement rate of pallid sturgeon was only one-half the peak rate of shovelnose sturgeon, but pallid sturgeon continued the lower rate for twice as long) and diel behavior of larvae (pallid sturgeon were diurnal and shovelnose sturgeon were nocturnal). Thus, the species used different methods to move the same distance. Migrating as poorly developed embryos suggests a migration style to avoid predation at the spawning site, but moving from spawning habitat to rearing habitat before first feeding could also be important. Migrants of both species preferred bright habitat (high illumination intensity and white substrate), a behavioral preference that may characterize the migrants of many species of sturgeon. Both species were remarkably similar for swimming height above the bottom by age, and day 7 and older migrants may swim far above the bottom and move far downstream. A migration of 12 or 13 days will probably not distribute larvae throughout the population's range, so an older life interval likely initiates a second longer downstream migration (2-step migration). By day 2, individuals of both species were a black-tail phenotype (light grey body with a black-tail that moved conspicuously during swimming). Aggregation behavior suggests that black-tail is a visual signal used for group cohesion.

Environmental Biology of Fishes↗

A food web including parasites for kelp forests of the Santa Barbara Channel, California

We built a high-resolution topological food web for the kelp forests of the Santa Barbara Channel, California, USA that includes parasites and significantly improves resolution compared to previous webs. The 1,098 nodes and 21,956 links in the web describe an economically, socially, and ecologically vital system. Nodes are broken into life-stages, with 549 free-living life-stages (492 species from 21 Phyla) and 549 parasitic life-stages (450 species from 10 Phyla). Links represent three kinds of trophic interactions, with 9,352 predator-prey links, 2,733 parasite-host links and 9,871 predator-parasite links. All decisions for including nodes and links are documented, and extensive metadata in the node list allows users to filter the node list to suit their research questions. The kelp-forest food web is more species-rich than any other published food web with parasites, and it has the largest proportion of parasites. Our food web may be used to predict how kelp forests may respond to change, will advance our understanding of parasites in ecosystems, and fosters development of theory that incorporates large networks.

California↗

A cytosolic carbonic anhydrase molecular switch occurs in the gills of metamorphic sea lamprey

Carbonic anhydrase plays a key role in CO 2 transport, acid-base and ion regulation and metabolic processes in vertebrates. While several carbonic anhydrase isoforms have been identified in numerous vertebrate species, basal lineages such as the cyclostomes have remained largely unexamined. Here we investigate the repertoire of cytoplasmic carbonic anhydrases in the sea lamprey ( Petromyzon marinus ), that has a complex life history marked by a dramatic metamorphosis from a benthic filter-feeding ammocoete larvae into a parasitic juvenile which migrates from freshwater to seawater. We have identified a novel carbonic anhydrase gene ( ca19 ) beyond the single carbonic anhydrase gene ( ca18 ) that was known previously. Phylogenetic analysis and synteny studies suggest that both carbonic anhydrase genes form one or two independent gene lineages and are most likely duplicates retained uniquely in cyclostomes. Quantitative PCR of ca19 and ca18 and protein expression in gill across metamorphosis show that the ca19 levels are highest in ammocoetes and decrease during metamorphosis while ca18 shows the opposite pattern with the highest levels in post-metamorphic juveniles. We propose that a unique molecular switch occurs during lamprey metamorphosis resulting in distinct gill carbonic anhydrases reflecting the contrasting life modes and habitats of these life-history stages.

Scientific Reports↗

Egg size variation among tropical and temperate songbirds: An embryonic temperature hypothesis

Species with 'slow' life history strategies (long life, low fecundity) are thought to produce high-quality offspring by investing in larger, but fewer, young. Larger eggs are indeed associated with fewer eggs across taxa and can yield higher-quality offspring. Tropical passerines appear to follow theory because they commonly exhibit slow life history strategies and produce larger, but fewer, eggs compared with northern species. Yet, I show here that relative egg mass (corrected for adult mass) varies extensively in the tropics and subtropics for the same clutch size, and this variation is unexplained. I propose a hypothesis to explain egg size variation both within the tropics and between latitudes: Relative egg mass increases in species with cooler egg temperatures and longer embryonic periods to offset associated increases in energetic requirements of embryos. Egg temperatures of birds are determined by parental incubation behavior and are often cooler among tropical passerines because of reduced parental attentiveness of eggs. Here, I show that cooler egg temperatures and longer embryonic periods explained the enigmatic variation in egg mass within and among regions, based on field studies in tropical Venezuela (36 species), subtropical Argentina (16 species), and north temperate Arizona (20 species). Alternative explanations are not supported. Thus, large egg sizes may reflect compensation for increased energetic requirements of cool egg temperatures and long embryonic periods that result from reduced parental attentiveness in tropical birds. ?? 2008 by The National Academy of Sciences of the USA.

Proceedings of the National Academy of Sciences of↗

Widespread thiamine deficiency in California salmon linked to an anchovy-dominated marine prey base

Thiamine (vitamin B 1 ) deficiency in marine systems is a globally significant threat to marine life. In 2020, newly hatched Chinook salmon ( Oncorhynchus tshawytscha ) fry in California’s Central Valley (CCV) hatcheries swam in corkscrew patterns and died at unusually high rates due to a lack of this essential vitamin. We subsequently investigated the impacts and causes of thiamine deficiency in California’s anadromous salmonids. Our laboratory studies defined the relationship between thiamine concentrations in Chinook salmon eggs and early life-stage survival in offspring; we used these data to develop a model that estimated 26 to 48% thiamine-dependent fry mortality across consecutive years (2020–2021) for winter-run Chinook salmon. We established an egg surveillance effort that found widespread thiamine deficiency in CCV Chinook salmon in 2020 and 2021, and emerging thiamine deficiency in Klamath River and Trinity River coho salmon ( Oncorhynchus kisutch ) in 2021. We determined that thiamine injections into adults raised egg thiamine concentrations above levels found to impact early life-stage survival and swimming behavior. Ocean surveys, prey nutrition, salmon gut contents, and stable isotope data link thiamine deficiency to an ocean diet dominated by a booming population of northern anchovy ( Engraulis mordax ). This forage fish had low thiamine, high lipid, and high thiaminase activity levels consistent with both a thiaminase and oxidative stress hypothesis for causing thiamine deficiency in California salmon. Our research suggests California’s already stressed anadromous salmonids will continue to be impacted by thiamine deficiency as long as their ocean forage base and diet are dominated by northern anchovy.

Proceedings of the National Academy of Sciences↗

Development of habitat suitability indices for the Candy Darter, with cross-scale validation across representative populations

Understanding relationships between habitat associations for individuals and habitat factors that limit populations is a primary challenge for managers of stream fishes. Although habitat use by individuals can provide insight into the adaptive significance of selected microhabitats, not all habitat parameters will be significant at the population level, particularly when distributional patterns partially result from habitat degradation. We used underwater observation to quantify microhabitat selection by an imperiled stream fish, the Candy Darter Etheostoma osburni , in two streams with robust populations. We developed multiple-variable and multiple-life-stage habitat suitability indices (HSIs) from microhabitat selection patterns and used them to assess the suitability of available habitat in streams where Candy Darter populations were extirpated, localized, or robust. Next, we used a comparative framework to examine relationships among (1) habitat availability across streams, (2) projected habitat suitability of each stream, and (3) a rank for the likely long-term viability (robustness) of the population inhabiting each stream. Habitat selection was characterized by ontogenetic shifts from the low-velocity, slightly embedded areas used by age-0 Candy Darters to the swift, shallow areas with little fine sediment and complex substrate, which were used by adults. Overall, HSIs were strongly correlated with population rank. However, we observed weak or inverse relationships between predicted individual habitat suitability and population robustness for multiple life stages and variables. The results demonstrated that microhabitat selection by individuals does not always reflect population robustness, particularly when based on a single life stage or season, which highlights the risk of generalizing habitat selection that is observed during nonstressful periods or for noncritical resources. These findings suggest that stream fish managers may need to be cautious when implementing conservation measures based solely on observations of habitat selection by individuals and that detailed study at the individual and population levels may be necessary to identify habitat that limits populations.

Transactions of the American Fisheries Society↗

Explaining reported puma-related behaviors and behavioral intentions among northern Arizona residents

Management of pumas in the American West is typified by conflict among stakeholders plausibly rooted in life experiences and worldviews. We used a mail questionnaire to assess demographics, nature-views, puma-related life experiences and behaviors, and support for puma-related policies among residents of northern Arizona. Data from the questionnaire (n = 693 respondents) were used to model behaviors and support for policies. Compared to models based on nature-views and life experiences, those based on demographics had virtually no support from the data. The Utilitarian/Dominionistic nature-view had the strongest effect of any variable in six of seven models, and was associated with firearms and opposition to policies that would limit killing pumas. The Humanistic/Moralistic nature-view was positively associated with non-lethal behaviors and policies in five models. Gender had the strongest effect of any demographic variable. Compared to demographics alone, our results suggest that worldviews provide a more meaningful explanation of reported human behaviors and behavioral intentions regarding pumas.

Arizona↗

Survival rates of birds of tropical and temperate forests: Will the dogma survive?

Survival rates of tropical forest birds are widely assumed to be high relative to the survival rates of temperate forest birds. Much life-history theory is based on this assumption despite the lack of empirical data to support it. We provide the first detailed comparison of survival rates of tropical and temperate forest birds based on extensive data bases and modern capture-recapture models. We find no support for the conventional wisdom. Because clutch size is only one component of reproductive rate, the frequently assumed, simple association between clutch size and adult survival rates should not necessarily be expected. Our results emphasize the need to consider components of fecundity in addition to clutch size when comparing the life histories of tropical and temperate birds and suggest similar considerations in the development of vertebrate life-history theory.

American Naturalist↗

Influence of sediment presence on freshwater mussel thermal tolerance

Median lethal temperature (LT50) data from water-only exposures with the early life stages of freshwater mussels suggest that some species may be living near their upper thermal tolerances. However, evaluation of thermal sensitivity has never been conducted in sediment. Mussels live most of their lives burrowed in sediment, so understanding the effect of sediment on thermal sensitivity is a necessary step in evaluating the effectiveness of the water-only standard method, on which the regulatory framework for potential thermal criteria currently is based, as a test of thermal sensitivity. We developed a method for testing thermal sensitivity of juvenile mussels in sediment and used the method to assess thermal tolerance of 4 species across a range of temperatures common during summer. Stream beds may provide a thermal refuge in the wild, but we hypothesized that the presence of sediment alone does not alter thermal sensitivity. We also evaluated the effects of 2 temperature acclimation levels (22 and 27°C) and 2 water levels (watered and dewatered treatments). We then compared results from the sediment tests to those conducted using the water-only standard methods. We also conducted water-only LT tests with mussel larvae (glochidia) for comparison with the juvenile life stage. We found few consistent differences in thermal tolerance between sediment and water-only treatments, between acclimation temperatures, between waterlevel treatments, among species, or between juvenile and glochidial life stages (LT50 range = 33.3-37.2°C; mean = 35.6°C), supporting our hypothesis that the presence of sediment alone does not alter thermal sensitivity. The method we developed has potential for evaluating the role of other stressors (e.g., contaminants) in a more natural and complex environment.

Freshwater Science↗

Recruitment phenology and pelagic larval duration in Caribbean amphidromous fishes

Amphidromous fishes are major components of oceanic tropical island stream ecosystems, such as those of the Caribbean island, Puerto Rico. Fishes with this life history face threats related to the requirement for connectivity between freshwater and marine environments during early life stages. Pelagic larval duration and recruitment phenology are 2 early life-history processes that are crucial for the biology, ecology, conservation, and management of amphidromous fishes. However, these processes are understudied in the Caribbean in general and have never been quantified in Puerto Rico. We quantified recruit abundance, recruitment phenology, and pelagic larval duration of several Caribbean amphidromous fish species in multiple rivers in Puerto Rico and explored the effects of environmental variables on recruit abundances. Two fish taxa—sirajo goby ( Sicydium spp.) and River Goby ( Awaous banana )—were exceptionally abundant as postlarvae and recruited to Caribbean rivers in pulsed migration episodes that were periodic at annual and lunar scales. Sirajo goby and River Goby recruit abundances varied among rivers, were greater at sunrise than at sunset, and were positively related to river discharge. The pelagic larval duration of 4 fish taxa ranged from a minimum of 28 d to a maximum of 103 d with means between 43 ± 7 d (SD) and 65 ± 11 d. We identified the last-quarter moon phase during the months of June through January as periods of maximum amphidromous fish recruitment to freshwater streams. The results and conclusions of our study can be applied to identify critical times to maintain river–ocean connectivity and stream flow for the benefit of the amphidromous fish population dynamics, stream ecology, and natural resources of the Caribbean.

Puerto Rico↗

Flow management for hydropower extirpates aquatic insects, undermining river food webs

Dams impound the majority of rivers and provide important societal benefits, especially daily water releases that enable on-peak hydroelectricity generation. Such “hydropeaking” is common worldwide, but its downstream impacts remain unclear. We evaluated the response of aquatic insects, a cornerstone of river food webs, to hydropeaking using a life history–hydrodynamic model. Our model predicts that aquatic-insect abundance will depend on a basic life-history trait—adult egg-laying behavior—such that open-water layers will be unaffected by hydropeaking, whereas ecologically important and widespread river-edge layers, such as mayflies, will be extirpated. These predictions are supported by a more-than-2500-sample, citizen-science data set of aquatic insects from the Colorado River in the Grand Canyon and by a survey of insect diversity and hydropeaking intensity across dammed rivers of the Western United States. Our study reveals a hydropeaking-related life history bottleneck that precludes viable populations of many aquatic insects from inhabiting regulated rivers.

BioScience↗

Suspended sediment and fisheries: An exploration of empirical relationships

Objective: Sediment has an important role in aquatic ecosystems, however, excess sediment can negatively impact fish and other aquatic life. Quantifying the response of aquatic life, particularly fish, to suspended sediment is important for natural resource managers tasked with developing sediment management guidelines to protect aquatic ecosystems. Our goal was to assess the ability of established, revised, and alternate severity of ill effect (SEV) dose-response models to predict the impact of suspended sediment on fish. Methods: We synthesized existing literature to develop an expansive dataset that relates suspended sediment concentration and exposure duration to biological effects on fishes and assessed the predictive ability of established and revised SEV dose-response models. We investigated potential sources of variation in biological responses to suspended sediment dose and explored two alternative approaches for assessing the effects of suspended sediment on fish: 90th quantile SEV dose-response regression models and logistic SEV dose-response models. Results: We found that both established and revised linear SEV dose-response models poorly quantify fish biological response to suspended sediment. Quantile SEV dose-response regressions also performed poorly. More promising are logistic dose-response models that identify sediment thresholds where major effects of sediment on fish can be expected to occur. We demonstrate that fish biological response to suspended sediment is modulated by sediment particle size, water temperature, and dissolved oxygen levels, suggesting additional environmental and biological variables to consider when evaluating the effects of suspended sediment on fish. Conclusion: We contribute revised and novel empirically derived tools for predicting the effects of suspended sediment on fish and demonstrate how environmental variables and life stage may modulate fish biological response. Our work illustrates challenges associated with predictive modeling and some potential sources of variation. While empirical models integrating biological stress response to suspended sediment may help natural resource managers capture potential impacts of this stressor, a cautious approach that considers co-acting stressors may be most effective for sediment management that is protective of fish.

North American Journal of Fisheries Management↗

Detecting population declines via monitoring the effective number of breeders (Nb)

Estimating the effective population size and effective number of breeders per year ( N b ) can facilitate early detection of population declines. We used computer simulations to quantify bias and precision of the one-sample LDNe estimator of N b in age-structured populations using a range of published species life history types, sample sizes, and DNA markers. N b estimates were biased by ~5%–10% when using SNPs or microsatellites in species ranging from fishes to mosquitoes, frogs, and seaweed. The bias (high or low) was similar for different life history types within a species suggesting that life history variation in populations will not influence N b estimation. Precision was higher for 100 SNPs ( H ≈ 0.30) than for 15 microsatellites ( H ≈ 0.70). Confidence intervals (CIs) were occasionally too narrow, and biased high when N b was small ( N b < 50); however, the magnitude of bias would unlikely influence management decisions. The CIs (from LDNe ) were sufficiently narrow to achieve high statistical power (≥0.80) to reject the null hypothesis that N b = 50 when the true N b = 30 and when sampling 50 individuals and 200 SNPs. Similarly, CIs were sufficiently narrow to reject N b = 500 when the true N b = 400 and when sampling 200 individuals and 5,000 loci. Finally, we present a linear regression method that provides high power to detect a decline in N b when sampling at least five consecutive cohorts. This study provides guidelines and tools to simulate and estimate N b for age structured populations ( https://github.com/popgengui/agestrucnb/ ), which should help biologists develop sensitive monitoring programmes for early detection of changes in N b and population declines.

Molecular Ecology Resources↗