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At least 829 records · Page 46Linked to original sources

Assessment of burrowing behavior of freshwater juvenile mussels in sediment

Standard laboratory sediment toxicity methods have been adapted for conducting toxicity tests with juvenile freshwater mussels. However, studies looking at juvenile mussel burrowing behavior at the water-sediment interface are limited. Juvenile mussels burrow in sediment for the first 0 to 4 yr of life but also may inhabit the sediment-water interface. The objective of this study was to evaluate burrowing behavior of various species and ages of juvenile freshwater mussels in three control sediments: West Bearskin Lake, Spring River, and coarse commercial sand. Species tested included (1) Fatmucket ( Lampsilis siliquoidea ), (2) Notched Rainbow ( Villosa constricta ), (3) Washboard ( Megalonaias nervosa ), (4) Rainbow ( Villosa iris) , (5) Arkansas Fatmucket ( Lampsilis powellii ), and (6) Oregon Floater ( Anodonta oregonensis ). Greater than 95% of the mussels burrowed into test sediment within 15 min. Across species, life stage, and substrate type, most mussels were recovered from the upper layers of sediment (91% at a sediment depth of 3.4 mm or less), and only 2% of the mussels were recovered at a depth >5.1 mm. No mussels were recovered from a depth >6.8 mm. There was no difference in mussel burrowing depth at 4 h versus 24 h across species, age, and sediment type. Two ages of Fatmucket burrowed to a significantly greater depth in the West Bearskin Lake sediment compared to the Spring River sediment or Coarse Sand. However, there was no significant difference in mean depth across sediment type with the other five species of mussels tested. Based on species and age of mussels tested, juvenile mussels up to an age of at least 20 wk and a length of at least 5 mm readily burrow into sediment and likely would be exposed to contaminants in whole sediment and associated pore water throughout a laboratory sediment toxicity test.

Freshwater Mollusk Biology and Conservation↗

Application of diagnostic tests for mycoplasmal infections of desert and gopher tortoises with management recommendations

Mycoplasmosis is a transmissible upper respiratory tract disease that has affected plans for management and conservation of wild desert and gopher tortoises in the United States. Although impact of mycoplasmosis on populations of desert and gopher tortoises is unknown, increased prevalence of seropositive animals as well as field observations of clinically ill tortoises have occurred in association with declining populations. In order to help in the identification of potentially infected animals, three tests have been developed to diagnose mycoplasmal infections of tortoises: 1) direct mycoplasmal culture; 2) detection of mycolplasmal chromosomal DNA by polymerase chain reaction (PCR); and 3) detection of anti-Mycoplasma antibodies in tortoise plasma by enzyme-linked immunosorbent assay (ELISA). Each test provides different, complementary information that collectively can be used to define tortoise mycoplasmal infection status. The types of samples required, the predictive value, interpretation, and cost vary among tests. These assays have been used for epidemiological surveys and in decision making for relocation, repatriation, or captive management of tortoises to minimize the risk of outbreaks of mycoplasmal respiratory disease and spread of the causative agent of this disease. Certain features of mycoplasmal infections of tortoises and other animals create a diagnostic dilemma. Multiple Mycoplasma species can cause respiratory disease with identical clinical presentations. Further, individual strains of a given species may vary with respect to their virulence potential, and some species may be commensals rather than pathogens. Current diagnostic tests may not differentiate among mycoplasmal species or strains or permit determination of pathogenicity of individual isolates. Thus, the information provided by testing is not a simple 'positive' vs. 'negative' issue. While these tests provide much needed information on the exposure of tortoise populations to Mycoplasma species, they do not provide a complete picture of the overall health status of individual tortoises or populations. Unfortunately, test results are often used to make life and death decisions concerning disposition of tortoises being displaced by land development without a complete understanding of the limitations of the diagnostic tests or any consideration of other infectious agents that might be present.

AZ, CA, FL, NV, UT↗

Population differentiation in Pacific salmon: local adaptation, genetic drift, or the environment?

Morphological, behavioral, and life-history differences between Pacific salmon (Oncorhynchus spp.) populations are commonly thought to reflect local adaptation, and it is likewise common to assume that salmon populations separated by small distances are locally adapted. Two alternatives to local adaptation exist: random genetic differentiation owing to genetic drift and founder events, and genetic homogeneity among populations, in which differences reflect differential trait expression in differing environments. Population genetics theory and simulations suggest that both alternatives are possible. With selectively neutral alleles, genetic drift can result in random differentiation despite many strays per generation. Even weak selection can prevent genetic drift in stable populations; however, founder effects can result in random differentiation despite selective pressures. Overlapping generations reduce the potential for random differentiation. Genetic homogeneity can occur despite differences in selective regimes when straying rates are high. In sum, localized differences in selection should not always result in local adaptation. Local adaptation is favored when population sizes are large and stable, selection is consistent over large areas, selective diffeentials are large, and straying rates are neither too high nor too low. Consideration of alternatives to local adaptation would improve both biological research and salmon conservation efforts.

Canadian Journal of Fisheries and Aquatic Sciences↗