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At least 793 records · Page 44Linked to original sources

Transport of microorganisms in the terrestrial subsurface: In situ and laboratory methods

This chapter describes and discusses laboratory and field techniques for studying microbial transport behavior in aquifer materials and model porous media. Changes in ionic strength (I) during transport studies may occur inadvertently as a result of using halides as conservative tracers and may lead to density-induced sinking of the tracer cloud. Substantive increases in I as a result of injection of high concentrations of halide tracers can also result in overestimations of microbial attachment. In order to differentiate "test" microorganisms from indigenous subsurface populations and/or from other inadvertently introduced populations, microorganisms used in laboratory or in situ transport tests are typically labeled a priori with a stable tag. Other methods of labeling microorganisms for use in in situ and column transport studies have involved the use of stable isotopes ratio mass spectrometry (IRMS). The characteristics of the conservative tracer breakthrough curve can then be used comparatively to determine some of the major transport parameters exhibited by the introduced microorganisms. Most controlled field investigations of subsurface microbial transport are conducted on limited spatial scales relative to the scales of interest to those concerned with pathogen transport to water supply wells, with microbially enhanced oil recovery from petroleum reservoirs, and with the feasibility of using introduced bacteria for aquifer restoration.

Book chapter↗

Techniques for estimating flood hydrographs for ungaged urban watersheds

The Clark Method, modified slightly was used to develop a synthetic, dimensionless hydrograph which can be used to estimate flood hydrographs for ungaged urban watersheds. Application of the technique results in a typical (average) flood hydrograph for a given peak discharge. Input necessary to apply the technique is an estimate of basin lagtime and the recurrence interval peak discharge. Equations for this purpose were obtained from a recent nationwide study on flood frequency in urban watersheds. A regression equation was developed which relates flood volumes to drainage area size, basin lagtime, and peak discharge. This equation is useful where storage of floodwater may be a part of design of flood prevention. (USGS)

Open-File Report↗

Element dispersion in alluvium covering gold deposits east of the Osgood Mountains, Getchell Trend, Humboldt County, Nevada: slides and text of a talk given at the Association of Exploration Geochemists' 15th International Geochemical Exploration Symposium, Reno, Nevada

The current trend in mineral exploration is to search for covered deposits. In the Great Basin, this translates into searching for ore bodies buried by alluvial cover. The exploration techniques used range from random drilling to the use of new and exciting geochemical sampling media and analytical methods. But we have a problem. Many of our geochemical techniques lack a conceptual basis (We tried it and it worked) or the original conceptual basis is now suspect (The method also works when it shouldn't). What we need is a basic understanding of what is going on in the alluvium -- the third dimension. The discovery and development of the alluvium-covered, disseminated gold deposits at Rabbit Creek, Chimney Creek, and Pinson provides us with an opportunity to gain some knowledge of the dispersion of elements in the third dimension. The work presented here is one part of a larger multi-disciplinary study of the Kelly Creek Valley by the U.S. Geological Survey in cooperation with FirstMiss Gold, Inc., Gold Fields Mining Corporation, Pinson Mining Company, and Santa Fe Pacific Mining, Inc.

Nevada↗

Herpetological Monitoring Using a Pitfall Trapping Design in Southern California

The steps necessary to conduct a pitfall trapping survey for small terrestrial vertebrates are presented. Descriptions of the materials needed and the methods to build trapping equipment from raw materials are discussed. Recommended data collection techniques are given along with suggested data fields. Animal specimen processing procedures, including toe- and scale-clipping, are described for lizards, snakes, frogs, and salamanders. Methods are presented for conducting vegetation surveys that can be used to classify the environment associated with each pitfall trap array. Techniques for data storage and presentation are given based on commonly use computer applications. As with any study, much consideration should be given to the study design and methods before beginning any data collection effort.

Techniques and Methods↗

The use of Galerkin finite-element methods to solve mass-transport equations

The partial differential equation that describes the transport and reaction of chemical solutes in porous media was solved using the Galerkin finite-element technique. These finite elements were superimposed over finite-difference cells used to solve the flow equation. Both convection and flow due to hydraulic dispersion were considered. Linear and Hermite cubic approximations (basis functions) provided satisfactory results: however, the linear functions were computationally more efficient for two-dimensional problems. Successive over relaxation (SOR) and iteration techniques using Tchebyschef polynomials were used to solve the sparce matrices generated using the linear and Hermite cubic functions, respectively. Comparisons of the finite-element methods to the finite-difference methods, and to analytical results, indicated that a high degree of accuracy may be obtained using the method outlined. The technique was applied to a field problem involving an aquifer contaminated with chloride, tritium, and strontium-90. (Woodard-USGS)

Water-Resources Investigations Report↗

On the use of the beta distribution in probabilistic resource assessments

The triangular distribution is a popular choice when it comes to modeling bounded continuous random variables. Its wide acceptance derives mostly from its simple analytic properties and the ease with which modelers can specify its three parameters through the extremes and the mode. On the negative side, hardly any real process follows a triangular distribution, which from the outset puts at a disadvantage any model employing triangular distributions. At a time when numerical techniques such as the Monte Carlo method are displacing analytic approaches in stochastic resource assessments, easy specification remains the most attractive characteristic of the triangular distribution. The beta distribution is another continuous distribution defined within a finite interval offering wider flexibility in style of variation, thus allowing consideration of models in which the random variables closely follow the observed or expected styles of variation. Despite its more complex definition, generation of values following a beta distribution is as straightforward as generating values following a triangular distribution, leaving the selection of parameters as the main impediment to practically considering beta distributions. This contribution intends to promote the acceptance of the beta distribution by explaining its properties and offering several suggestions to facilitate the specification of its two shape parameters. In general, given the same distributional parameters, use of the beta distributions in stochastic modeling may yield significantly different results, yet better estimates, than the triangular distribution.

Natural Resources Research↗

Calculated geochronology and stress field orientations along the Hawaiian chain

A new method has been discovered for calculating ages of the main shield building stages of volcanoes along the Hawaiian chain from Kilauea to the Hawaiian-Emperor bend. The method is based on a graphical technique for hypothetical subtraction of distance intervals that theoretically represent regions of simultaneous volcanism along adjacent or nearly en-echelon loci of volcanism. Distances along the chain, measured from Kilauea, when progressively foreshortened by the distances of hypothetical “collapse” and plotted versus existing age data are found to give linear age-distance relationships. A calibration graph is presented that agrees closely with the measured ages in 17 of the 20 existing dated volcanoes. The criterion for simultaneous activity on different loci is based on the concept of equal azimuths of synchronous volcanic propagation within coeval segments of the chain. This is the predicted relationship when magmatic fluids inject the lithosphere along directions normal to a nearly horizontal least principal stress. It appears that the Pacific plate has been subjected to oscillatory, but principally clockwise, rotations of horizontal stress components during the last 40 m.y.

Hawaii↗

The San Andreas Fault System--Complexities along a major transform fault system and relation to earthquake hazards

The San Andreas Fault System is a 1300-km-long transform boundary that accommodates motion between the North American and Pacific Plates. New technologies and data reveal rich details about the present configuration of faults, distribution of strain and associated seismic hazard on this complex network of faults. This contribution provides a brief summary of the geologic history of the San Andreas Fault System, followed by an introduction to recent research that has changed understanding of the hazards along the main faults. Organized by region, we highlight a selection of recent research using new geodetic techniques, improved topographic data, advanced geochronologic methods, and high-resolution geophysics. The contribution ends with a review of the historic earthquakes on the San Andreas and San Jacinto Faults, comparing these to past ruptures interpreted from paleoseismic studies.

California↗

A Visual Basic program to plot sediment grain-size data on ternary diagrams

Sedimentologic datasets are typically large and compiled into tables or databases, but pure numerical information can be difficult to understand and interpret. Thus, scientists commonly use graphical representations to reduce complexities, recognize trends and patterns in the data, and develop hypotheses. Of the graphical techniques, one of the most common methods used by sedimentologists is to plot the basic gravel, sand, silt, and clay percentages on equilateral triangular diagrams. This means of presenting data is simple and facilitates rapid classification of sediments and comparison of samples. The original classification scheme developed by Shepard (1954) used a single ternary diagram with sand, silt, and clay in the corners and 10 categories to graphically show the relative proportions among these three grades within a sample. This scheme, however, did not allow for sediments with significant amounts of gravel. Therefore, Shepard's classification scheme was later modified by the addition of a second ternary diagram with two categories to account for gravel and gravelly sediment (Schlee, 1973). The system devised by Folk (1954, 1974)\ is also based on two triangular diagrams, but it has 21 categories and uses the term mud (defined as silt plus clay). Patterns within the triangles of both systems differ, as does the emphasis placed on gravel. For example, in the system described by Shepard, gravelly sediments have more than 10% gravel; in Folk's system, slightly gravelly sediments have as little as 0.01% gravel. Folk's classification scheme stresses gravel because its concentration is a function of the highest current velocity at the time of deposition as is the maximum grain size of the detritus that is available; Shepard's classification scheme emphasizes the ratios of sand, silt, and clay because they reflect sorting and reworking (Poppe et al., 2005). The program described herein (SEDPLOT) generates verbal equivalents and ternary diagrams to characterize sediment grain-size distributions. It is written in Microsoft Visual Basic 6.0 and provides a window to facilitate program execution. The inputs for the sediment fractions are percentages of gravel, sand, silt, and clay in the Wentworth (1922) grade scale, and the program permits the user to select output in either the Shepard (1954) classification scheme, modified as described above, or the Folk (1954, 1974) scheme. Users select options primarily with mouse-click events and through interactive dialogue boxes. This program is intended as a companion to other Visual Basic software we have developed to process sediment data (Poppe et al., 2003, 2004).

Computers & Geosciences↗

Development of ‘SedCam’— A close-range remote sensing method of estimating suspended-sediment concentration in small rivers

The adaptation of suspended-sediment surrogate technologies continues to rapidly expand across geomorphology and fluvial sediment monitoring efforts. Over a decade of research and development shows increased reliability and accuracy of in-situ surrogates with reduced program cost as compared to traditional sample-based methods, but environmental fouling and probe damage can be problematic. The SedCam technique is a unique non-contact close-range remote sensing method to estimate suspended-sediment concentration from multispectral imagery of a river surface. In contrast to typical airborne- or satellite-based platforms, SedCam uses broadband sensors with lower spectral resolution (three bands covering wavelengths of 340 to 1100 nm) but greater spatial resolution (0.5 mm pixel size; equivalent to medium to coarse sand) and temporal resolution (15-min intervals during daylight hours). This paper summarizes lessons learned from two studies, utilizing three consumer-grade digital cameras (each with different spectral signatures) at two different rivers (each with different sediment characteristics). >90,000 images and 174 concurrent physical samples represent a collective period of 26 months. A subset of these data pairs supports the development of four regression models. Statistical diagnostics show model error can be <40 % when surface point samples are used, with coefficients of determination ≥0.90. This novel approach shows similar accuracy to other surrogate methods such as instream turbidity. Results of this study indicate that optimizing spectra based on expected suspended-sediment concentration increases model performance.

Geomorphology↗

Measurement of dissolved organic matter fluorescense in aquatic environments: An interlaboratory comparison

The fluorescent properties of dissolved organic matter (DOM) are often studied in order to infer DOM characteristics in aquatic environments, including source, quantity, composition, and behavior. While a potentially powerful technique, a single widely implemented standard method for correcting and presenting fluorescence measurements is lacking, leading to difficulties when comparing data collected by different research groups. This paper reports on a large-scale interlaboratory comparison in which natural samples and well-characterized fluorophores were analyzed in 20 laboratories in the U.S., Europe, and Australia. Shortcomings were evident in several areas, including data quality-assurance, the accuracy of spectral correction factors used to correct EEMs, and the treatment of optically dense samples. Data corrected by participants according to individual laboratory procedures were more variable than when corrected under a standard protocol. Wavelength dependency in measurement precision and accuracy were observed within and between instruments, even in corrected data. In an effort to reduce future occurrences of similar problems, algorithms for correcting and calibrating EEMs are described in detail, and MATLAB scripts for implementing the study's protocol are provided. Combined with the recent expansion of spectral fluorescence standards, this approach will serve to increase the intercomparability of DOM fluorescence studies.

Environmental Science & Technology↗

Infrasound signal detection and back azimuth estimation using ground-coupled airwaves on a seismo-acoustic sensor pair

We present a new infrasonic signal detection and back azimuth determination technique that requires just one microphone and one three-component seismometer. Ground-coupled airwaves (GCAs) occur when an incident atmospheric acoustic wave impinges on the ground surface and is partially transmitted as a seismic wave. GCAs are commonly detected hundreds of kilometers away on seismic networks and are observed to have retrograde particle motion. Horizontally propagating acoustic waves and GCAs have previously been observed on collocated infrasound and seismic sensor pairs as coherent with a 90° phase difference. If the sensors are spatially separated, an additional propagation-induced phase shift is present. The additional phase shift depends on the direction from which the acoustic wave arrives, as each back azimuth has a different apparent distance between the sensors. We use the additional phase shift, the coherence, and the characteristic particle motion on the three-component seismometer to determine GCA arrivals and their unique back azimuth. We test this technique with synthetic seismo-acoustic data generated by a coupled Earth-atmosphere 3-D finite difference code, as well as three seismo-acoustic data sets from Mount St. Helens, Mount Cleveland, and Mount Pagan volcanoes. Results from our technique compare favorably with traditional infrasound array processing and provide robust GCA detection and back azimuth determination. Assuming adequate station spacing and sampling, our technique provides a new and robust method to detect infrasonic signals and determine their back azimuth, and may be of practical benefit where resources are limited and large sensor networks or arrays are not feasible.

JGR Solid Earth↗

Procedures for woody vegetation surveys in the Kazgail rural council area, Kordofan, Sudan

Efforts to reforest parts of the Kordofan Province of Sudan are receiving support from international development agencies. These efforts include planning and implementing reforestation activities that require the collection of natural resources and socioeconomic data, and the preparation of base maps. A combination of remote sensing, geographic information system and global positioning systems procedures are used in this study to meet these requirements. Remote sensing techniques were used to provide base maps and to guide the compilation of vegetation resources maps. These techniques provided a rapid and efficient method for documenting available resources. Pocket‐sized global positioning system units were used to establish the location of field data collected for mapping and resource analysis. A microcomputer data management system tabulated and displayed the field data. The resulting system for data analysis, management, and planning has been adopted for the mapping and inventory of the Gum Belt of Sudan.

Geocarto International↗

Induction of antibody-producing cells in rainbow trout, Salmo gairdneri Richardson, by flush exposure

Splenic antibody-producing cells were produced by rainbow trout that had been exposed to O-antigens extracted from Yersinia ruckeri and Aeromonas salmonicida by adding the concentrated antigen preparation directly into the water of the tank holding the fish for a flush exposure. This method was compared with the proven techniques of exposure: intraperitoneal injection or a 2 minute immersion of the fish in the antigen preparation. Dosage experiments showed that the production of antibody-producing cells was induced by the immersion of trout for 2 minutes in water with 5.0 μg/ml-1 (or more) with the Y. ruckeri O-antigen, or 500 μg ml-1 (or more) of the A. salmonicida O-antigen. Similar differences were evident when the respective antigens were added directly to the water.

Journal of Fish Biology↗

Infectious Cryptosporidium parvum oocysts in final reclaimed effluent

Water samples collected throughout several reclamation facilities were analyzed for the presence of infectious Cryptosporidium parvum by the focus detection method-most-probable-number cell culture technique. Results revealed the presence of infectious C. parvum oocysts in 40% of the final disinfected effluent samples. Sampled effluent contained on average seven infectious oocysts per 100 liters. Thus, reclaimed water is not pathogen free but contains infectious C. parvum.

Applied and Environmental Microbiology↗

Application of a hollow-fiber, tangential-flow device for sampling suspended bacteria and particles from natural waters

The design and application of a hollow-fiber tangential-flow filtration device has been used to concentrate bacteria and suspended particles from large volume surface water and groundwater samples (i.e., hundreds of liters). Filtrate tlux rates (4&ndash;8 L min &minus;1 ) are equal to or faster than those of other devices that are based on continuous flow centrifugation and plate and frame filtration. Particle recovery efficiencies for inorganic particles (approximately 90%) were similar to other dewatering devices, but microbial cell recoveries (30&ndash;90%) were greatly improved by this technique relative to other currently available methods. Although requirements for operation and maintenance of the device are minimal, its size, as with other dewatering devices, limits its applicability at remote sample sites. Nevertheless, it has proven useful for sample collection in studies involving microbial transport and analysis of particle-associated trace inorganic solutes.

Journal of Environmental Quality↗

Implanting intra-abdominal radiotransmitters with external whip antennas in ducks

We developed and evaluated a surgical procedure for implanting intra-abdominal radiotransmitters with external whip antennas in captive mallards ( Anas platyrhynchos ). Transmitters were implanted in the abdominal cavity and the antennas exited through the caudal abdominal wall and skin. Birds with implanted transmitters developed mild to moderate localized air sac reactions. These reactions involved adhesions of the right anterior abdominal air sac to the liver with contractions around the transmitters and antenna catheters. The adhesions were reinforced by a proliferation of connective tissue and lined by multinucleated giant cells (foreign body reaction). Casual observation indicated that neither behavior nor activity of the birds was altered by the histological reaction to the transmitter implant. No increase in systemic lesions (particularly liver or kidney) could be correlated with the histological reactions. Our evaluations indicate that the procedure is a reliable method for radiomarking ducks and the technique has been successfully used in 2 field studies.

Journal of Wildlife Management↗