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Map showing radon potential of rocks and soils in Fairfax County, Virginia

Since 1984, indoor radon has gained national attention as a significant health hazard in the United States. Radon is a colorless, odorless, radioactive gas derived from uranium by radioactive decay. The U.S. Environmental Protection Agency (EPA) now projects that 5,000 to 20,000 lung-cancer deaths per year may be attributed to the long-term exposure to indoor radon and its radioactive decay products. Indoor radon has been previously recognized as a health hazard associated with uranium-bearing mill tailings or building materials, but it was not until December 1984 that some natural soils and rocks were found to be sources of indoor radon at levels comparable to those in uranium mines. It is now suspected that elevated indoor radon levels are far more widespread than initially though. The EPA considers 4 picoCuries of radon per liter of air (pCi/L) as the level (in a year-round measurement) at which actions ought to be taken to lower the concentration of indoor radon. All soils and rocks contain measurable amounts of uranium, which generate measurable amounts of radon. Certain soils and rocks, however, have a greater potential to cause indoor radon problems than others because (1) they have a higher uranium content and thus can generate higher levels of radon in soil gas (gas that occupies the pores of the soil), and (2) the permeability of the sol or rack is sufficiently high that radon-bearing soil gas can flow freely and move indoors through the foundation of the structure. This study was designed to demonstrate the correlation between the geologic environment and indoor radon levels and to demonstrate a method of assessment that could be used by other informed workers in areas of their interest. A parallel study by Gundersen and others (1988) of the radon potential of rocks and soils in Montgomery County, Md., used somewhat different methods of assessment because the data available for and assessment of Montgomery County differed.

Virginia↗

Microbial and viral indicators of pathogens and human health risks from recreational exposure to waters impaired by fecal contamination

Fecal indicator bacteria (FIB) (e.g., fecal coliforms, Escherichia coli , and enterococci) have been used for decades to monitor for and protect the public from waterborne pathogens from fecal contamination. However, FIB may not perform well at predicting the presence of waterborne pathogens or human health outcomes from recreational exposure to fecal-contaminated surface waters. Numerous factors can influence the relationship between FIB and pathogens or human health outcomes, including the source(s) of contamination, the type of pathogen(s) present, differences in the survival and behavior of FIB and pathogens in the wastewater conveyance and treatment process, and varying environmental conditions. As a result, different indicators, such as source-specific microbial source tracking (MST) markers and viral fecal indicators, have been used as possible surrogates to better approximate pathogen abundance and human health risks in recreational waters. The performance of these alternative indicators has been mixed, with some promise of viral indicators better approximating viral pathogens than bacterial fecal indicators, and FIB generally more closely associated with bacterial and protozoal pathogen presence than human MST markers. Many of the assays to detect and quantify fecal indicators and pathogens are polymerase chain reaction-based assays, which detect and quantify nucleic acid [deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)] sequences specific to a target of interest. Recent advances in DNA and RNA sequencing technologies may push the field toward metabarcoding approaches, where multiple targets can be detected and quantified simultaneously. Metabarcoding is currently more applicable to bacterial and protozoal assessments than viral assessments based on a lack of universal metabarcoding markers for viruses. Innovative technologies, such as biosensors and nanotechnologies, may provide more sensitive and accurate tools to detect and quantify pathogens. When a specific pathogen is of concern for a recreational water body, a practical approach in estimating the likelihood of human health outcomes is the application of quantitative microbial risk assessments (QMRAs). Quantitative microbial risk assessments can be used to model the likelihood of pathogen-specific human health outcomes from recreational exposure as a function of a surrogate indicator. Inputs for QMRAs include the ratio between the indicator to be monitored and the pathogen of interest, the concentration of the indicator, the amount of water ingested, and the likelihood of the health outcome based on the estimated amount of pathogen consumed. There are numerous unknowns about the behavior and survival of fecal indicators and pathogens in environmental waters. Developing accurate models to predict pathogen concentrations from fecal indicators in recreational waters will require a better understanding of these unknowns. Current methods and technologies for detecting and quantifying fecal indicators and pathogens are limited due to the rare and patchy nature of pathogens. Technological advances may help improve sensitivity for detecting and quantifying pathogens.

Journal of Sustainable Water in the Built Environm↗

Ecological status of aquatic communities in selected streams in the Milwaukee Metropolitan Sewerage District planning area of Wisconsin, 2004–13

A total of 14 wadable streams in urban or urbanizing watersheds near Milwaukee, Wisconsin, were sampled in 2004, 2007, 2010, and 2013 to assess the ecological status of aquatic communities (biota), including benthic algae and invertebrates, and fish. To assess temporal variation, additional community sampling was also done at a subset of three sites in 2011 and 2012. Relative abundances of each type of organism were used to calculate biological metrics, such as richness and diversity, percentages of intolerant and tolerant organisms, and indexes of biotic integrity for invertebrates and fish. Selected environmental (physical and chemical) data in the streams were collected to evaluate potential relations to the biota and the ecological health of the stream. Physical and chemical data included land use/land cover, stream discharge from U.S. Geological Survey (USGS) streamgages (except at 2 creeks that were not gaged), stream habitat, microhabitat at invertebrate collection locations, water quality (except at 2 creeks that were not gaged), field measurements of several water-quality constituents, measures of benthic algal biomass, and toxicity and chemical tests on extracts from passive samplers deployed at a subset of 6 sites. Relative abundances of organisms and biological metrics were compared among sampling years and with environmental metrics to evaluate the ecological status of these streams and determine primary stressors on the aquatic communities, with the aim of helping resource managers understand and work toward improving the ecological health of these and other urban and urbanizing rivers in the study area. Biological metrics for most sites indicated some level of diminished ecological status when compared across all sampled sites and when compared with rating scales for selected metrics. The least degraded sites among all those sampled—indicated by aggregate bioassessments for algae, invertebrates, and fish metrics and in order starting with the best overall condition—were the Milwaukee River near Cedarburg, Menomonee River at Menomonee Falls, Jewel Creek, and Milwaukee River at Milwaukee. The most degraded sites were Menomonee River at Wauwatosa, Root River at Greenfield, Lincoln Creek, and the Kinnickinnic River. Differences in aggregate bioassessments indicate that aquatic communities at the Menomonee River at Wauwatosa site and the Root River at Greenfield site were worse in 2013 than in 2004; however, Oak Creek and Honey Creek sites were better. In 2013, several sites had less than 30-percent pollution-sensitive diatoms indicating degraded algal assemblages. Invertebrate metrics for most of the 14 sites in 2013 were lower than in 2004 and indicate that invertebrate assemblages at most sampled sites were more degraded in 2013. Tolerant fish taxa made up more than 40 percent of assemblages at most sites and nearly 100 percent of assemblages at four sites. At times, in some smaller streams, too few fish were captured to compute an Index of Biotic Integrity with confidence, and invertebrates provided a better means for assessing the ecological status and water quality. With these few exceptions, the use of all three groups of biota provided the most robust assessments at the 14 sites in 2004–13. Physical and chemical stressors were correlated to adverse effects on aquatic biota at the sampled streams. Passive samplers were deployed at a subset of six sites in 2013. Microtox results indicated there was little or no toxicity at the Milwaukee River near Cedarburg site and at the Oak Creek site, slight toxicity at the Lincoln Creek and Honey Creek sites, and moderate toxicity at the Milwaukee River at Milwaukee site and the Little Menomonee River site; however, based on cytochrome-P450 reporter gene system toxicity tests, potential toxicity from hydrophobic organic contaminants was measured at all six sites. For all 14 sites, physical and chemical stressors related to urbanization correlated with biological metrics for algae, invertebrates, and fish. Most stressors for aquatic biota reflected an urban signature. Stressors related to ecological condition in our study were chemical and physical, such as developed land, impervious surface in the watershed, urban land in a buffer area around the stream (a 100-foot [30-meter]-wide area on each side of the stream, and maximum instantaneous discharge normalized by drainage area (a measure of flood and scour effects). Chemical stressors included low waterborne concentrations of dissolved oxygen and high concentrations of chloride, zinc and other metals, nutrients (nitrite and phosphorus), and fecal coliform bacteria. Although algae, invertebrates, and fish did not always demonstrate a significant response to the same stressors, higher abundances of high total phosphorus-indicator diatoms, lower ratings for invertebrate biotic integrity indexes and percentages of mayflies-stoneflies-caddisflies, and lower values for fish biotic integrity indexes underscored possible adverse effects of even low levels of developed land. Developed land is typically associated with more rapid runoff, which washes chemicals from impervious surfaces into area waterways and degrades stream habitat for aquatic communities. However, with respect to at least chloride from road salt, diatoms tolerant to dissolved salts were significantly lower with as little as 1-percent mixed forest in the watershed. Lower percentages of urban land in the stream buffer correlated with healthier aquatic assemblages of algae, invertebrates, and fish. The assessment of algal, invertebrate, and fish assemblages coupled with physical and chemical data were highly useful in evaluating the ecological status of aquatic communities at the 14 sites and for determining environmental stressors that may be contributing to reduced stream condition. Some of the stressors could potentially be removed or lessened with stream rehabilitation or changes in watershed management.

Wisconsin↗

The U.S. Geological Survey Ecosystem Science Strategy, 2012-2022 - Advancing discovery and application through collaboration

Ecosystem science is critical to making informed decisions about natural resources that can sustain our Nation’s economic and environmental well-being. Resource managers and policy-makers are faced with countless decisions each year at local, state, tribal, territorial, and national levels on issues as diverse as renewable and non-renewable energy development, agriculture, forestry, water supply, and resource allocations at the urban-rural interface. The urgency for sound decision-making is increasing dramatically as the world is being transformed at an unprecedented pace and in uncertain directions. Environmental changes are associated with natural hazards, greenhouse gas emissions, and increasing demands for water, land, food, energy, mineral, and living resources. At risk is the Nation’s environmental capital, the goods and services provided by resilient ecosystems that are vital to the health and well-being of human societies. Ecosystem science—the study of systems of organisms interacting with their environment and the consequences of natural and human-induced change on these systems—is necessary to inform decision-makers as they develop policies to adapt to these changes. This Ecosystems Science Strategy is built on a framework that includes basic and applied science. It highlights the critical roles that USGS scientists and partners can play in building scientific understanding and providing timely information to decision-makers. The strategy underscores the connection between scientific discoveries and the application of new knowledge. The strategy integrates ecosystem science and decision-making, producing new scientific outcomes to assist resource managers and providing public benefits. The USGS is uniquely positioned to play an important role in ecosystem science. With its wide range of expertise, the agency can bring holistic, cross-scale, interdisciplinary capabilities to the design and conduct of monitoring, research, and modeling and to new technologies for data collection, management, and visualization. Collectively, these capabilities can be used to reveal ecological patterns and processes, explain how and why ecosystems change, and forecast change over different spatial and temporal scales. USGS science can provide managers with options and decision-support tools to use resources sustainably. The USGS has long-standing, collaborative relationships with the DOI and other partners in the natural sciences, in both conducting science and its application. The USGS engages these partners in cooperative investigations that otherwise would lack the necessary support or be too expensive for a single bureau to conduct. The heart of this strategy is a framework and vision for USGS ecosystems science that focuses on five long-term goals, which are seen as interconnected and reinforcing components: • Improve understanding of ecosystem structure, function, and processes. The focus for this goal is an understanding of how ecosystems work, including the dynamics of species, their populations, interactions, and genetics, and how they change across spatial and temporal scales. • Advance understanding of how drivers influence ecosystem change. The challenges here are explaining the drivers of ecosystem change, their spatio-temporal patterns, their uncertainties and interactions, and their influence on ecosystem processes and dynamics. • Improve understanding of the services that ecosystems provide to society. Here the emphasis is on the measurement of environmental capital and ecosystem services, and the identification of sources and patterns of change in space and time. • Develop tools, technologies, and capacities to inform decision-making about ecosystems. This includes developing new technologies and approaches for conducting applications-oriented ecosystem science. A principal challenge will be how to quantify uncertainty and incorporate it in decision analysis. • Apply science to enhance strategies for management, conservation, and restoration of ecosystems. These challenges include development of novel approaches to monitoring, assessment, and restoration of ecosystems; new methods to address species of concern and communities at risk; and innovations in decision analysis and support to address imminent ecosystem changes or those that are underway. Closely integrated with the five goals are four strategic approaches that provide the path forward for the USGS Ecosystems Mission Area. These approaches cross-cut all of the goals and are seen as essential to the implementation of this strategy: • Assess information needs for ecosystem science through enhanced partnerships. Work with the DOI and other agencies and institutions to identify, design, and implement priority decision-driven ecological research. • Promote the use of interdisciplinary ecosystem science. Design and conduct interdisciplinary process-oriented research in ecosystem science. • Enhance modeling and forecasting. Build models to forecast ecosystem change, assess future management scenarios, and reduce uncertainties through an adaptive learning process. • Support decision-making. Use quantitative approaches to assess the vulnerabilities of ecosystems, habitats, and species, and evaluate strategies for adaptation, restoration, and sustainable management. Following the strategic approaches are a set of proposed actions that represent a sampling of specific activities that align with this strategy and that address the Nation’s most pressing environmental needs. The strategy emphasizes coordination of activities across the USGS mission areas pursuant to these goals. Ecosystem science is inherently interdisciplinary and requires a broad perspective that incorporates the biological and physical sciences, climate science, information technology, and scientific capacity in mission areas across the Bureau. With its emphasis on coordination, this strategy can provide a critical underpinning for integrated science efforts with scientists from multiple mission areas of the USGS working together. Of course, the USGS will continue to conduct both discipline-specific and interdisciplinary investigations, and both will continue to be vital parts of the ecosystem science portfolio. Finally, the strategy stresses the importance of coordination with other Federal agencies and organizations in the natural resources community. The USGS collaborates with resource agencies in the DOI and other organizations throughout the world to meet societal needs for species and ecosystem management. Working with these agencies and organizations, the USGS will play a key role over the next decade in advancing the scientific foundation for sustaining the natural resources that diverse, productive, resilient ecosystems provide.

Open-File Report↗

Developing integrated methods to address complex resource and environmental issues

Introduction This circular provides an overview of selected activities that were conducted within the U.S. Geological Survey (USGS) Integrated Methods Development Project, an interdisciplinary project designed to develop new tools and conduct innovative research requiring integration of geologic, geophysical, geochemical, and remote-sensing expertise. The project was supported by the USGS Mineral Resources Program, and its products and acquired capabilities have broad applications to missions throughout the USGS and beyond. In addressing challenges associated with understanding the location, quantity, and quality of mineral resources, and in investigating the potential environmental consequences of resource development, a number of field and laboratory capabilities and interpretative methodologies evolved from the project that have applications to traditional resource studies as well as to studies related to ecosystem health, human health, disaster and hazard assessment, and planetary science. New or improved tools and research findings developed within the project have been applied to other projects and activities. Specifically, geophysical equipment and techniques have been applied to a variety of traditional and nontraditional mineral- and energy-resource studies, military applications, environmental investigations, and applied research activities that involve climate change, mapping techniques, and monitoring capabilities. Diverse applied geochemistry activities provide a process-level understanding of the mobility, chemical speciation, and bioavailability of elements, particularly metals and metalloids, in a variety of environmental settings. Imaging spectroscopy capabilities maintained and developed within the project have been applied to traditional resource studies as well as to studies related to ecosystem health, human health, disaster assessment, and planetary science. Brief descriptions of capabilities and laboratory facilities and summaries of some applications of project products and research findings are included in this circular. The work helped support the USGS mission to “provide reliable scientific information to describe and understand the Earth; minimize loss of life and property from natural disasters; manage water, biological, energy, and mineral resources; and enhance and protect our quality of life.” Activities within the project include the following: Spanned scales from microscopic to planetary; Demonstrated broad applications across disciplines; Included life-cycle studies of mineral resources; Incorporated specialized areas of expertise in applied geochemistry including mineralogy, hydrogeology, analytical chemistry, aqueous geochemistry, biogeochemistry, microbiology, aquatic toxicology, and public health; and Incorporated specialized areas of expertise in geophysics including magnetics, gravity, radiometrics, electromagnetics, seismic, ground-penetrating radar, borehole radar, and imaging spectroscopy. This circular consists of eight sections that contain summaries of various activities under the project. The eight sections are listed below: Laboratory Facilities and Capabilities, which includes brief descriptions of the various types of laboratories and capabilities used for the project; Method and Software Development, which includes summaries of remote-sensing, geophysical, and mineralogical methods developed or enhanced by the project; Instrument Development, which includes descriptions of geophysical instruments developed under the project; Minerals, Energy, and Climate, which includes summaries of research that applies to mineral or energy resources, environmental processes and monitoring, and carbon sequestration by earth materials; Element Cycling, Toxicity, and Health, which includes summaries of several process-oriented geochemical and biogeochemical studies and health-related research activities; Hydrogeology and Water Quality, which includes descriptions of innovative geophysical, remote-sensing, and geochemical research pertaining to hydrogeology and water-quality applications; Hazards and Disaster Assessment, which includes summaries of research and method development that were applied to natural hazards, human-caused hazards, and disaster assessments; and Databases and Framework Studies, which includes descriptions of fundamental applications of geophysical studies and of the importance of archived data.

Circular↗

U.S. Geological Survey ecosystems science strategy—Advancing discovery and application through collaboration

Executive Summary Ecosystem science is critical to making informed decisions about natural resources that can sustain our Nation’s economic and environmental well-being. Resource managers and policymakers are faced with countless decisions each year at local, regional, and national levels on issues as diverse as renewable and nonrenewable energy development, agriculture, forestry, water supply, and resource allocations at the urban-rural interface. The urgency for sound decisionmaking is increasing dramatically as the world is being transformed at an unprecedented pace and in uncertain directions. Environmental changes are associated with natural hazards, greenhouse gas emissions, and increasing demands for water, land, food, energy, mineral, and living resources. At risk is the Nation’s environmental capital, the goods and services provided by resilient ecosystems that are vital to the health and wellbeing of human societies. Ecosystem science—the study of systems of organisms interacting with their environment and the consequences of natural and human-induced change on these systems—is necessary to inform decisionmakers as they develop policies to adapt to these changes. This Ecosystems Science Strategy is built on a framework that includes basic and applied science. It highlights the critical roles that U.S. Geological Survey (USGS) scientists and partners can play in building scientific understanding and providing timely information to decisionmakers. The strategy underscores the connection between scientific discoveries and the application of new knowledge, and it integrates ecosystem science and decisionmaking, producing new scientific outcomes to assist resource managers and providing public benefits. We envision the USGS as a leader in integrating scientific information into decisionmaking processes that affect the Nation’s natural resources and human well-being. The USGS is uniquely positioned to play a pivotal role in ecosystem science. With its wide range of expertise, the Bureau can bring holistic, cross-scale, interdisciplinary capabilities to the design and conduct of monitoring, research, and modeling and to new technologies for data collection, management, and visualization. Collectively, these capabilities can be used to reveal ecological patterns and processes, explain how and why ecosystems change, and forecast change over different spatial and temporal scales. USGS science can provide managers with options and decision-support tools to use resources sustainably. The USGS has long-standing, collaborative relationships with the Department of the Interior (DOI) and other partners in the natural sciences, in both conducting science and applying the results. The USGS engages these partners in cooperative investigations that otherwise would lack the necessary support or be too expensive for a single bureau to conduct. The heart of this strategy is a framework for USGS ecosystems science that focuses on five long-term goals, which are seen as interconnected components that reinforce our vision of the USGS providing science that is at the forefront of decisionmaking: Improve understanding of ecosystem structure, function, and processes. The focus for this goal is an understanding of how ecosystems work, including the dynamics of species, their populations, interactions, and genetics, and how they change across spatial and temporal scales. Advance understanding of how drivers influence ecosystem change. The challenges here are explaining the drivers of ecosystem change, their spatio-temporal patterns, their uncertainties and interactions, and their influence on ecosystem processes and dynamics. Improve understanding of the services that ecosystems provide to society. Here the emphasis is on the measurement of environmental capital and ecosystem services, and the identification of sources and patterns of change in space and time. Develop tools, technologies, and capacities to inform decisionmaking about ecosystems. This includes developing new technologies and approaches for conducting applications-oriented ecosystem science. A principal challenge will be how to quantify uncertainty and incorporate it in decision analysis. Apply science to enhance strategies for management, conservation, and restoration of ecosystems. These challenges include development of novel approaches to monitoring, assessment, and restoration of ecosystems; new methods to address species of concern and communities at risk; and innovations in decision analysis and support to address imminent ecosystem changes or those that are underway. Closely integrated with the five goals are four strategic approaches that provide the path forward for the USGS Ecosystems Mission Area. These approaches cross-cut all of the goals and are seen as essential to the implementation of this strategy: Assess information needs for ecosystem science through enhanced partnerships. Work with the DOI and other agencies and institutions to identify, design, and implement priority decision-driven ecological research. Promote the use of interdisciplinary ecosystem science. Design and conduct interdisciplinary process-oriented research in ecosystem science. Enhance modeling and forecasting. Build models to forecast ecosystem change, assess future management scenarios, and reduce uncertainties through an adaptive learning process. Support decisionmaking. Use quantitative approaches to assess the vulnerabilities of ecosystems, habitats, and species, and evaluate strategies for adaptation, restoration, and sustainable management. Following the four strategic approaches are a set of proposed actions that represent a sampling of specific USGS activities that align with this strategy and that address the Nation’s most pressing environmental needs. The strategy emphasizes coordination of activities across the USGS mission areas pursuant to these goals. Ecosystem science is inherently interdisciplinary and requires a broad perspective that incorporates the biological and physical sciences, climate science, information technology, and scientific capacity in mission areas across the Bureau. With its emphasis on coordination, this strategy can provide a critical underpinning for integrated science efforts with scientists from multiple mission areas of the USGS working together. Of course, the USGS will continue to conduct discipline-specific and interdisciplinary investigations, and both will continue to be vital parts of the ecosystem science portfolio. Finally, the strategy stresses the importance of coordination with other Federal agencies and organizations in the natural resources community. The USGS collaborates with resource agencies in the DOI and other organizations throughout the world to meet societal needs for species and ecosystem management. Working with these agencies and organizations, the USGS will play a key role in guiding sound decisionmaking during the next decade by advancing the scientific foundation for sustaining the natural resources that diverse, productive, resilient ecosystems provide.

Circular↗

Endocrine-disrupting activity of hydraulic fracturing chemicals and adverse health outcomes after prenatal exposure in male mice

Oil and natural gas operations have been shown to contaminate surface and ground water with endocrine-disrupting chemicals. In the current study, we fill several gaps in our understanding of the potential environmental impacts related to this process. We measured the endocrine-disrupting activities of 24 chemicals used and/or produced by oil and gas operations for five nuclear receptors using a reporter gene assay in human endometrial cancer cells. We also quantified the concentration of 16 of these chemicals in oil and gas wastewater samples. Finally, we assessed reproductive and developmental outcomes in male C57BL/6J mice after the prenatal exposure to a mixture of these chemicals. We found that 23 commonly used oil and natural gas operation chemicals can activate or inhibit the estrogen, androgen, glucocorticoid, progesterone, and/or thyroid receptors, and mixtures of these chemicals can behave synergistically, additively, or antagonistically in vitro. Prenatal exposure to a mixture of 23 oil and gas operation chemicals at 3, 30, and 300 μg/kg · d caused decreased sperm counts and increased testes, body, heart, and thymus weights and increased serum testosterone in male mice, suggesting multiple organ system impacts. Our results suggest possible adverse developmental and reproductive health outcomes in humans and animals exposed to potential environmentally relevant levels of oil and gas operation chemicals.

Colorado↗

Sampling protocol for post-landfall Deepwater Horizon oil release, Gulf of Mexico, 2010

The protocols and procedures described in this report are designed to be used by U.S. Geological Survey (USGS) field teams for the collection of environmental data and samples in coastal areas affected by the 2010 Deepwater Horizon oil spill in the Gulf of Mexico. This sampling protocol focuses specifically on sampling for water, sediments, benthic invertebrates, and microorganisms (ambient bacterial populations) after shoreline arrival of petroleum-associated product on beach, barrier island, and wetland environments of the Gulf of Mexico coastal states. Deployment to sampling sites, site setup, and sample collection in these environments necessitates modifications to standard USGS sampling procedures in order to address the regulatory, logistical, and legal requirements associated with samples collected in oil-impacted coastal areas. This document, therefore, has been written as an addendum to the USGS National Field Manual for the Collection of Water-Quality Data (NFM) (http://pubs.water.usgs.gov/twri9A/), which provides the basis for training personnel in the use of standard USGS sampling protocols. The topics covered in this Gulf of Mexico oil-spill sampling protocol augment NFM protocols for field-deployment preparations, health and safety precautions, sampling and quality-assurance procedures, and decontamination requirements under potentially hazardous environmental conditions. Documentation procedures and maintenance of sample integrity by use of chain-of-custody procedures also are described in this protocol.

Open-File Report↗

Chapel Hill bisphenol A expert panel consensus statement: Integration of mechanisms, effects in animals and potential to impact human health at current levels of exposure

This document is a summary statement of the outcome from the meeting: “ Bisphenol A: An Examination of the Relevance of Ecological, In vitro and Laboratory Animal Studies for Assessing Risks to Human Health ” sponsored by both the NIEHS and NIDCR at NIH/DHHS, as well as the US-EPA and Commonweal on the estrogenic environmental chemical bisphenol A (BPA, 2,2-bis(4-hydroxyphenyl)propane; CAS# 80-05-7). The meeting was held in Chapel Hill, NC, 28–30 November 2006 due to concerns about the potential for a relationship between BPA and negative trends in human health that have occurred in recent decades. Examples include increases in abnormal penile/urethra development in males, early sexual maturation in females, an increase in neurobehavioral problems such as attention deficit hyperactivity disorder (ADHD) and autism, an increase in childhood and adult obesity and type 2 diabetes, a regional decrease in sperm count, and an increase in hormonally mediated cancers, such as prostate and breast cancers. Concern has been elevated by published studies reporting a relationship between treatment with “low doses” of BPA and many of theses negative health outcomes in experimental studies in laboratory animals as well as in vitro studies identifying plausible molecular mechanisms that could mediate such effects. Importantly, much evidence suggests that these adverse effects are occurring in animals within the range of exposure to BPA of the typical human living in a developed country, where virtually everyone has measurable blood, tissue and urine levels of BPA that exceed the levels produced by doses used in the “low dose” animal experiments.

Reproductive Toxicology↗

African dust carries microbes across the ocean: are they affecting human and ecosystem health?

Atmospheric transport of dust from northwest Africa to the western Atlantic Ocean region may be responsible for a number of environmental hazards, including the demise of Caribbean corals; red tides; amphibian diseases; increased occurrence of asthma in humans; and oxygen depletion (eutrophication) in estuaries. Studies of satellite images suggest that hundreds of millions of tons of dust are trans-ported annually at relatively low altitudes across the Atlantic Ocean to the Caribbean Sea and southeastern United States. The dust emanates from the expanding Sahara/Sahel desert region in Africa and carries a wide variety of bacteria and fungi. The U.S. Geological Survey, in collaboration with the NASA/Goddard Spaceflight Center, is conducting a study to identify microbes--bacteria, fungi, viruses--transported across the Atlantic in African soil dust. Each year, millions of tons of desert dust blow off the west African coast and ride the trade winds across the ocean, affecting the entire Caribbean basin, as well as the southeastern United States. Of the dust reaching the U.S., Florida receives about 50 percent, while the rest may range as far north as Maine or as far west as Colorado. The dust storms can be tracked by satellite and take about one week to cross the Atlantic.

Open-File Report↗

Occurrence of organic wastewater contaminants, pharmaceuticals, and personal care products in selected water supplies, Cape Cod, Massachusetts, June 2004

In June 2004, the U.S. Geological Survey, in cooperation with the Barnstable County Department of Health and Environment, sampled water from 14 wastewater sources and drinking-water supplies on Cape Cod, Massachusetts, for the presence of organic wastewater contaminants, pharmaceuticals, and personal care products. The geographic distribution of sampling locations does not represent the distribution of drinking-water supplies on Cape Cod. The environmental presence of the analyte compounds is mostly unregulated; many of the compounds are suspected of having adverse ecological and human health effects. Of the 85 different organic analyte compounds, 43 were detected, with 13 detected in low concentrations (less than 1 microgram per liter) from drinking-water supplies thought to be affected by wastewater because of previously detected high nitrate concentrations. (Phenol and d-limonene, detected in equipment blanks at unacceptably high concentrations, are not included in counts of detections in this report.) Compounds detected in the drinking-water supplies included the solvent, tetrachloroethylene; the analgesic, acetaminophen; the antibiotic, sulfamethoxazole; and the antidepressant, carbamazapine. Nitrate nitrogen, an indicator of wastewater, was detected in water supplies in concentrations ranging from 0.2 to 8.8 milligrams per liter.

Open-File Report↗

A ground electromagnetic survey used to map sulfides and acid sulfate ground waters at the abandoned Cabin Branch Mine, Prince William Forest Park, northern Virginia gold-pyrite belt

INTRODUCTION AND BACKGROUND: Prince William Forest Park is situated at the northeastern end of the Virginia Gold-Pyrite belt northwest of the town of Dumfries, VA. The U. S. Marine Corps Reservation at Quantico borders the park on the west and south, and occupies part of the same watershed. Two abandoned mines are found within the park: the Cabin Branch pyrite mine, a historic source of acid mine drainage, and the Greenwood gold mine, a source of mercury contamination. Both are within the watershed of Quantico Creek (Fig.1). The Cabin Branch mine (also known as the Dumfries mine) lies about 2.4 km northwest of the town of Dumfries. It exploited a 300 meter-long, lens-shaped body of massive sulfide ore hosted by metamorphosed volcanic rocks; during its history over 200,000 tons of ore were extracted and processed locally. The site became part of the National Capitol Region of the National Park Service in 1940 and is currently managed by the National Park Service. In 1995 the National Park Service, in cooperation with the Virginia Department of Mines, Minerals, and Energy reclaimed the Cabin Branch site. The Virginia Gold-Pyrite belt, also known as the central Virginia volcanic-plutonic belt, is host to numerous abandoned metal mines (Pavlides and others, 1982), including the Cabin Branch deposit. The belt itself extends from its northern terminus near Cabin Branch, about 50 km south of Washington, D.C., approximately 175 km to the southwest into central Virginia. It is underlain by metamorphosed volcanic and clastic (non-carbonate) sedimentary rocks, originally deposited approximately 460 million years ago during the Ordovician Period (Horton and others, 1998). Three kinds of deposits are found in the belt: volcanic-associated massive sulfide deposits, low-sulfide quartz-gold vein deposits, and gold placer deposits. The massive sulfide deposits such as Cabin Branch were historically mined for their sulfur, copper, zinc, and lead contents, but also yielded byproduct gold and silver. The environmental impact of massive sulfide deposits can be substantial. These deposits are characterized by high concentrations of heavy-metal sulfide minerals, hosted by silicate rocks. Thus, weathering of these deposits and their mine wastes has the potential to generate heavy-metal laden sulfuric acid that can have negative impacts on aquatic ecosystems. In addition, lead associated with solid mine wastes has the potential for human health impacts through ingestion. The heavy metals that are encountered in these deposits and are most likely to cause environmental impacts include copper, zinc, lead, cadmium, and arsenic. In addition, the weathering of pyrite releases large amounts of iron, and the acid generated attacks the country rocks and causes the release of large amounts of aluminum, which also can severely impact aquatic ecosystems. A reclamation attempt was made at the site in 1995, including construction of storm-water diversion trenches around the abandoned mine area, grading tailings away from the stream bank, addition of pulverized limestone and topsoil, and revegetation. The post-reclamation chemistry of shallow groundwaters (<3 meters deep) shows a neutral pH on the southwestern bank of the stream but pH of 4.1 to 4.5 on the northeastern bank. The dominant ions are Fe2+ and SO42- (Seal, Haffner, Meier, and Pollio, 1999) A ground electromagnetic survey was conducted over the site in 1999 as part of a wider study ( Seal, Haffner, and Meier, 1998a,b, 1999). It was hoped that a 3-D map of the soil conductivity derived from the survey could provide insight into the distribution of the mobilized sulfides present under the ground.

Virginia↗

Development and Application of Health-Based Screening Levels for Use in Water-Quality Assessments

Health-Based Screening Levels (HBSLs) are non-enforceable water-quality benchmarks that were developed by the U.S. Geological Survey in collaboration with the U.S. Environmental Protection Agency (USEPA) and others. HBSLs supplement existing Federal drinking-water standards and guidelines, thereby providing a basis for a more comprehensive evaluation of contaminant-occurrence data in the context of human health. Since the original methodology used to calculate HBSLs for unregulated contaminants was published in 2003, revisions have been made to the HBSL methodology in order to reflect updates to relevant USEPA policies. These revisions allow for the use of the most recent, USEPA peer-reviewed, publicly available human-health toxicity information in the development of HBSLs. This report summarizes the revisions to the HBSL methodology for unregulated contaminants, and updates the guidance on the use of HBSLs for interpreting water-quality data in the context of human health.

Scientific Investigations Report↗

Chemical mixtures in potable water in the U.S.

In recent years, regulators have devoted increasing attention to health risks from exposure to multiple chemicals. In 1996, the US Congress directed the US Environmental Protection Agency (EPA) to study mixtures of chemicals in drinking water, with a particular focus on potential interactions affecting chemicals' joint toxicity. The task is complicated by the number of possible mixtures in drinking water and lack of toxicological data for combinations of chemicals. As one step toward risk assessment and regulation of mixtures, the EPA and the Agency for Toxic Substances and Disease Registry (ATSDR) have proposed to estimate mixtures' toxicity based on the interactions of individual component chemicals. This approach permits the use of existing toxicological data on individual chemicals, but still requires additional information on interactions between chemicals and environmental data on the public's exposure to combinations of chemicals. Large compilations of water-quality data have recently become available from federal and state agencies. This chapter demonstrates the use of these environmental data, in combination with the available toxicological data, to explore scenarios for mixture toxicity and develop priorities for future research and regulation. Occurrence data on binary and ternary mixtures of arsenic, cadmium, and manganese are used to parameterize the EPA and ATSDR models for each drinking water source in the dataset. The models' outputs are then mapped at county scale to illustrate the implications of the proposed models for risk assessment and rulemaking. For example, according to the EPA's interaction model, the levels of arsenic and cadmium found in US groundwater are unlikely to have synergistic cardiovascular effects in most areas of the country, but the same mixture's potential for synergistic neurological effects merits further study. Similar analysis could, in future, be used to explore the implications of alternative risk models for the toxicity and interaction of complex mixtures, and to identify the communities with the highest and lowest expected value for regulation of chemical mixtures.

Book chapter↗

Mountain sentinels in a changing world: Review and conservation implications of weather and climate effects on mountain goats (Oreamnos americanus)

Climate change is occurring at an accelerated rate in high-elevation alpine and mountain ecosystems. Cold-adapted, mountain species are at risk due to forecasted change and knowledge is needed to respond to current and future conservation challenges. Mountain goats ( Oreamnos americanus ) are an iconic species of North American mountain cultures and landscapes, and due to specialized adaptations for life in cold, mountainous environments they are particularly sensitive to changes in weather and climate. As sentinels of change in alpine ecosystems, the study of mountain goats offers insight into the ecological effects and conservation challenges associated with climate change in these sensitive and biodiverse environments. Here, we synthesize existing knowledge about how climate change is expected to influence environmental conditions experienced by mountain goats and associated mechanistic changes to behavior, nutritional ecology, demography, health, and interspecific interactions. In many instances, climate change effects are likely to be negative and additive to existing threats (such as human disturbance, hunting, disease, predation) though benefits are expected in some cases. Changes in climate and mountain environments will necessitate re-examination and modification of population monitoring, management, and conservation strategies. Specifically, spatiotemporal (and other) aspects of monitoring and management may need to be adjusted to accommodate emerging and novel conservation challenges. Yet, key data and knowledge gaps remain and should be addressed to advance conservation and decision-making capabilities. For mountain goats and similarly climate-sensitive alpine herbivores, effective conservation will ultimately benefit from collaborations among diverse networks guided by well-planned, strategic visions focused on common ground – namely the resiliency and persistence of culturally and ecologically significant mountain species and the alpine environment they inhabit.

Alaska, Alberta, British Columbia, Idaho, Montana,↗

Ranking river basins for stream temperature research and monitoring in the contiguous United States

There is a need to prioritize research and data collection in river basins by integrating information from environmental, ecological, and socioeconomic datasets to maintain acceptable water quality for human uses and ecosystem health. Multiple anthropogenic and natural stressors are responsible for driving changes in stream temperatures that can alter ecosystems and degrade water quality. These stressors are variable spatially and temporally, which can be challenging for prioritizing monitoring and research. In this study, an evaluation of variables related to stream temperature was performed for 163 candidate basins of the contiguous United States to highlight potential focal areas. Thirty variables were combined in six components: anthropogenic forcing, natural forcing, climate change, ecological sensitivity, socioeconomic sensitivity, and data availability. The components were incorporated into three themes: vulnerability of streams to thermal change, vulnerability of ecosystems, and vulnerability of communities. By evaluating each theme separately, patterns of vulnerability and potential resiliency were identified as well as consistency in ranks within the themes. For example, results of the national scale ranking indicated the Southern California Coastal basin (within California-Nevada region) was the highest-ranking priority in vulnerability of streams to thermal change and vulnerability of ecosystems. The analysis also identified vulnerable basins with gaps in monitoring. For example, the Missouri-Oahe basin (within the Northern High Plains region) was the highest-ranking priority for vulnerable communities with limitations in data availability. The ranking approach provides insight into basins that are resilient and are ideal candidates for monitoring and research.

Journal of Hydrology↗

Interleukin-6 and tumor necrosis factor-alpha values in elk neonates

Serological indicators of general condition would be helpful for monitoring or assessing ungulate wildlife. Toward that end, we report the 1st reference values for 2 cytokines, interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-??), in neonatal elk (Cervus elaphus). We obtained blood samples from 140 calves ??? 6 days old in Yellowstone National Park during summer 2003-2005. TL-6 values ranged from 0 to 1.21 pg/ml with a median of 0.03 pg/ml. TNF-?? values ranged from 0 to 225.43 pg/ml with a median of 1.85 pg/ml. IL-6 and TNF-?? concentrations were not significant predictors of elk calf survival through 21 days. Development of ungulate-based IL-6 and TNF-?? assays that provide greater sensitivity than cross-reacting human-based assays could be helpful in monitoring ungulate condition and health status comparisons among herds. Such information could provide indirect assessments of range quality or environmental influences among herds.

Journal of Mammalogy↗