USGS Science⌕ Search

SEARCH · USGS Science

Results for “Blood”

Search indexed USGS publications on groundwater, aquifers, geologic maps, mineral resources and earthquakes. Explore source records by subject and place.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 793 records · Page 44Linked to original sources

Medea genes, handedness and other traits

Medea factors or genes are maternal-effects mechanisms, found in many species, in which the mother's body selectively kills embryos of a certain genotype.Humans have a similar genetic mechanism, the gene RHD which produces Rh-factor involved in blood type.Recently I proposed that RHD acts as a maternal-effects gene that determines handedness (i.e., right handed or non-right handed) in individuals of our species. Here, I argue that RHD functions as a Medea gene as well.The handedness gene (and also RHD itself in some cases) has been implicated in autism spectrum disorders (ASD), bipolar disorder, cerebral laterality (i.e., right-brained or left-brained speech laterality), hair-whorl rotation, schizophrenia, sexual orientation, and speech dyslexia.Identifying the gene or genes that determine handedness or cerebral laterality may help uncover the mechanisms underlying these behavioral phenotypes in our species.A relatively simple test of the handedness hypothesis has been proposed:In a sample of humans for whom handedness has been evaluated, we would need to genotype for RHD by determining whether Rh+ individuals have one or two copies of the dominant allele. If RHD and perhaps also an interaction with RHCE are involved in sexual orientation, it explains how selection could favor a gene or genes which cause some people to become non-heterosexual.The literature on Medea genes provides the explanation:A Medea allele must increase in frequency, sometimes to fixation (i.e., 100% frequency) even if it reduces fecundity (e.g., birth rate).In addition, treatment for RHD maternal-fetal genotype incompatibility, which allows more fetuses to survive to term now, may be one explanation for why ASD appears to be increasing in frequency in some populations, if RHD is indeed the handedness gene, although many other mechanisms have also been suggested. One wonders if bipolar disorder and the other alternative phenotypes are also increasing in frequency.

Journal of Sleep Disorders & Therapy↗

West nile virus in American white pelican chicks: transmission, immunity, and survival

West Nile virus (WNV) causes significant mortality of American White Pelican chicks at northern plains colonies. We tested oropharyngeal/cloacal swabs from moribund chicks for shed WNV. Such shedding could enable chick-to-chick transmission and help explain why WNV spreads rapidly in colonies. WNV was detected on swabs from 11% of chicks in 2006 and 52% of chicks in 2007; however, viral titers were low. Before onset of WNV mortality, we tested blood from < 3-week-old chicks for antibodies to WNV; 5% of chicks were seropositive, suggesting passive transfer of maternal antibodies. Among near-fledged chicks, 41% tested positive for anti-WNV antibodies, indicating that they survived infection. Among years and colonies, cumulative incidence of WNV in chicks varied from 28% to 81%, whereas the proportion of chicks surviving WNV (i.e., seropositive) was 64–75%. Our data revealed that WNV kills chicks that likely would fledge in the absence of WNV, that infection of chicks is pervasive, and that significant numbers of chicks survive infection.

American Journal of Tropical Medicine and Hygiene↗

A lead isotope distribution study in swine tissue using ICP-MS

In the United States lead is an ubiquitous environmental pollutant that is a serious human health hazard, especially for women of childbearing age, developing fetuses, and young children. Information concerning the uptake and distribution of lead to maternal and fetal tissues during pregnancy is poorly documented. A study was designed using domestic swine and lead isotope enrichment methodology to focus on maternal absorption and distribution of lead into bone and soft tissues, including the fetal compartment, under varying conditions of oral lead exposure and during altered physiological states (pregnant vs unbred). Total lead levels and Pb207/Pb206 ratios in bone (femur and vertebra), blood, and soft tissues (liver, kidney, brain) were determined by ICP-MS. Lead in fetal tissues derived from maternal bone could be differentiated from that derived from exogenous dosing. Unbred swine absorbed much less lead than pregnant females receiving the same dose. The accuracy and precision of ICP-MS at the instrumental level and for the entire method (sample collection, digestion, and analysis) were evaluated for both Pb207/Pb206 ratios and total lead. Several changes were suggested in method design to improve both instrumental and total method precision.

Atomic Spectroscopy↗

An introduction to standardized sampling

It was probably one of the oddest riots in the history of the United States. In Erie, Pennsylvania during 1853. federal marshals were called to restore order during bloody uprisings. A mob of women, equipped with sledgehammers, was tearing up railroad rack to protest standardization of track width (Nesmith 1985). All across the United States, standardization of rail gauges was talking place to improve transportation across the country,but many people did not want consistency. Jobs moving freight from, a train running on one gauge of track to a train running on another gauge were plentiful at this time, and standardization would mean these jobs would disappear. Fortunately, for us today, the riots were quelled and standardization of railroad tack gauges went ahead. The magnificent transportation system of North America was aided by the standardization of rails, contributing to robust economies. Standardization of industrial processes, languages, measurements, and data collection methods has been essential for world progress (Figure 1.1). Today , we are often unaware of the degree of standardization of the most basic elements of our society--from bolts and nuts where thread sizes are standard to computer components that can be used interchangeably to the standard sizes of photos we carry in our wallets or purses. Data collection and presentation are standardized in many disciplines, including medicine, meteorology, geology, and water chemistry. For example, our cholesterol, body temperature, and blood pressure are measured by standard medical tests and compared to averages calculated from the results of the same standard tests for many other people to determine if individuals are higher, lower, or average compared to the population in general. If these diagnostic tests were not standardized, it is unlikely that we would be able to evaluate eve the most basic data about our health. In fact, if standardization was not used in countless other facets of our society our lives would be much more difficult. For data collection purposes, standardization means to collect data in one way so comparisons can be easily made. Although routine data collection has been standardized in many other disciplines, data from freshwater fish sampling across North America have not. Previously, most data collection has been standardized only at local, state, and provincial levels (Bonar and Hubert 2002). Several years ago, when one of the authors (Bonar) was a biologist for a state agency, he was asked to compile as much data as he could about the state's warmwater fish communities to provide information to managers developing fishing regulations. These data had been collected by many biologists over time using different methods, including rotenone, electrofishing, gill netting, and hook-and-line sampling. Data were written carefully on detailed data sheets or in scribbled notes in a biologists's notebook. As you can imagine, these data were a nightmare to compile. However, they were even worse to interpret. How could length-frequency distributions be compared among lakes if the methods used to catch the fish were dissimilar with differing efficiencies in sampling fish of various species and lengths? How could catch per unit effort (CPUE), a common index of population density, be compared when samples were collected one year using fyke nets and the next year by electrofishing? Ultimately, how could one compare if fish population were high, low, or average in growth, body condition, or abundance if there was no compilation of distributions of standard data to facilitate comparison? Months were spent trying to interpret these data, and finally a body of comparable data gathered by similar methods was assimilated. However, much of the nonstandard data had to be discarded--data that had taken thousands of hours to collect but were essentially useless. If all data jhad been collected and recorded in a standard manner, whoch would have required very little extra work, all of these hours of survey effort would not have gone to naught and isights regardimng the fisheries would have been imporived by a larger number of samples.

Book chapter↗

Fatal pox infection in a rough-legged hawk

Natural pox infection occurred in a free-living rough-legged hawk ( Buteo lagopus ) in northeastern North Dakota. Gross, histological and electron microscopic findings were typical of pox infection, and characteristic lesions developed in red-tailed hawks ( Buteo jamaicensis ) but not in great horned owls ( Bubo virginianus ) following inoculation with case material. Death of the rough-legged hawk was attributed to starvation resulting from inability to capture prey and to blood loss from foot lesions.

Journal of Wildlife Diseases↗

Leucocytozoonosis in Canada Geese at the Seney National Wildlife Refuge

A history is given of the Seney National Wildlife Refuge and the losses of goslings of Canada geese ( Branta canadensis ) recorded since inception of the refuge in 1935. Since 1960, when more reliable data became available, losses have been extensive every 4 years. Gosling deaths are attributed to the infection with Leucocytozoon simondi . The blackfly ( Simulium innocens ) is considered to be the prime vector in the transmission of this blood parasite to goslings.

Michigan↗

Detection and transmission of infectious hematopoietic necrosis virus in rainbow trout

Detection and transmission of Infectious Hematopoietic Necrosis Virus in rainbow trout ( Salmo gairdneri ) was studied at a commercial trout hatchery. Transmission of virus was demonstrated via water, feed and contaminated eggs. If eggs from carrier females were incubated several weeks in virus-free water, the resulting fry did not become infected. However, if fry subsequently became infected they were lifetime carriers. Infectious virus was readily detectable in most tissues of moribund fish; in carriers it was detected in sex products of spawning fish, and in samples from the intestine of post-spawning fish, but not in samples from blood, feces, kidney, or liver. The carrier rate was not significantly different between sexes. It was concluded that adult carriers are the reservoir of infection and that transmission occurs primarily when carriers shed virus and expose susceptible fish or eggs.

Journal of Wildlife Diseases↗

Serologic and hematologic values of wild coyotes in Wisconsin

Blood samples were obtained from 30 coyotes (Canis latrans) captured in northern Wisconsin in conjunction with radio-telemetry studies. Samples were assayed for seven hematologic values, seven serum chemistries, serum albumin, globulin and total protein. Results are given with respect to sex and age and are compared with available data for captive wild and pen-raised coyotes. Leukocyte counts were greater for males than females and packed cell volumes were greater for adults than young, possibly due to differential response to capture and handling stress. Hemoglobin concentrations and calcium levels suggest differences in nutrition between pen-raised and wild coyotes. Sex and age differences in serum calcium for wild coyotes probably reflect nutritional differences between groups examined. Juvenile coyote serum alkaline phosphatase levels declined curvilinearly with age for coyotes less than one year old, suggesting a possible technique for separating juveniles and yearlings captured in autumn that are released for research purposes. Elevated glucose levels and leukocyte counts in wild coyotes may reflect greater handling stress than for pen-raised and captive coyotes. No significant sex or age effects were found for levels of serum urea nitrogen, total protein, cholesterol, and total bilirubin.

Journal of Wildlife Diseases↗

Evaluation of coproexamination as a diagnostic test for avian botulism

Fecal extracts and blood sera from 113 ducks showing clinical signs of botulism were examined for Clostridium botulinum type C toxin by means of the mouse toxicity test to evaluate coproexamination as a diagnostic procedure, as compared with demonstration of toxin in serum. When death of test mice unprotected with type specific antitoxin (while protected controls survived) was the criterion, 78.8% of the sera and 5.3% of the fecal extracts were positive. When characteristic signs of intoxication in the unprotected mice was included as evidence of toxin in the specimens, these percentages increased to 86.7 and 6.2, respectively. Fecal specimens were collected hourly for the first 6 h after peroral dosing of eight mallards ( Anas platyrhynchos ) with 1.0 LD 50 , of type C toxin and at 24, 48, and 72 h from birds surviving that long. From 2 to 4 toxin-positive specimens were passed by all eight ducks during the first 6 h, five specimens were positive at 24 h, and three were positive at 48 h. Only three specimens were collected at 72 h, all of which were negative. These findings suggest that attempts to detect toxin in the feces of wild ducks might have been more successful had the birds been captured earlier in the course of the disease.

Utah↗

Tissue lead distribution and hematologic effects in American kestrels (Falco sparverius) fed biologically incorporated lead

American kestrels were fed a diet containing 0.5, 120, 212, and 448 ppm (dry wt) biologically incorporated lead (Pb) for 60 days. The diet consisted of homogenized 4-wk-old cockerels raised on feed mixed with and without lead. No kestrels died and weights did not differ among treatment groups. The control group (0.5 ppm Pb) had the lowest mean concentration of lead and the high dietary group had the highest for the following tissues: Kidney, liver, femur, brain, and blood. Concentrations of lead were significantly correlated among tissues. There were no differences among treatment groups for packed cell volume, hemoglobin concentration, or erythrocyte count.

Journal of Wildlife Diseases↗

An outbreak of type E botulism among common loons ( Gavia immer ) in Michigan's Upper Peninsula

An epizootic of type E botulism (Clostridium botulinum) occurred among common loons (Gavia immer) along the Lake Michigan shore of Michigan's Upper Peninsula (USA) during October and November 1983. An estimated 592 dead loons washed ashore along the Garden Peninsula. Type E botulinal toxin was demonstrated in blood samples and stomach contents of dead loons, and in samples of three species of dead fish found on the Lake Michigan shore. We suspect that loons acquired botulism by ingesting sick or dead fish containing type E toxin.

Michigan↗

Serologic response of Rio Grande wild turkeys to experimental infections of Mycoplasma gallisepticum

The serologic response of Rio Grande wild turkeys (Meleagris gallopavo intermedia) to Mycoplasma gallisepticum (MG) was determined. Free-ranging turkeys were caught in southern Texas, shipped to the University of Wisconsin, Madison, and housed in isolation facilities. Fourteen birds were exposed to MG, by intratracheal and intranasal inoculation. Eight birds received sterile broth only. Two wk prior to the end of the experiment, MG exposed turkeys were stressed by challenge with a serologically unrelated mycoplasma. Serum from all exposed birds reacted positively for MG antibody by the rapid plate agglutination (RPA) procedure within 2 mo postexposure (PE) and all but one remained positive for 14 mo PE. Less than one half of the exposed birds developed positive MG antibody titers detectable by the hemagglutination inhibition (HI) test within 2 mo PE, and by 10 mo PE, none had positive titers. Antibody was detected by the HI test in two of 11 infected turkeys, 14 mo PE, and titers increased significantly within 2 wk. MG was isolated from tracheal swabs from two infected birds 2 mo PE, but attempts thereafter failed. However, at the termination of the experiment 15 mo later, MG was isolated from lung tissue of three of 11 exposed turkeys and from a blood clot found in the lower trachea of one bird.

Texas↗

Immune response of mallard ducks treated with immunosuppressive agents: Antibody response to erythrocytes and in vivo response to phytohemagglutinin-P

The ability of two in vivo tests to assay immune competence of mallard ducks ( Anas platyrhynchos ) treated with various immunomodulatory agents was examined. Skin responses to phytohemagglutinin-P (PHA-P) injected intradermally and serum antibody levels produced in response to sheep red blood cells (SRBC) were measured. As measured by the skin response to PHA-P, ducks injected intramuscularly with cyclophosphamide or cyclosporine did not respond differently from control-injected ducks. Dexamethasone injected intramuscularly significantly suppressed the skin response to PHA-P. As measured by antibody levels in response to SRBC, ducks injected intramuscularly with cyclophosphamide responded with antibody titers similar to controls. Cyclosporine injected intramuscularly reduced the level of immunoglobulin (Ig) G significantly in one of two experiments. Dexamethasone injected intramuscularly reduced peak total and IgG titers. These experiments provide information on the viability of these two in vivo tests to reflect immune competence of mallard ducks.

Journal of Wildlife Diseases↗

Gray wolf density and its association with weights and hematology of pups from 1970 to 1988

We examined weights and hematologic profiles of gray wolf ( Canis lupus ) pups and the associated wolf density in the east-central Superior National Forest of northeastern Minnesota (USA) during 1970 to 1988. We collected weight and hematologic data from 117 pups (57 females, 60 males) during 1 September to 22 November each year. The wolf density (wolves/800 km 2 ) trend was divided into three phases: high (72 ± 7), 1970 to 1975; medium (44 ± 2), 1976 to 1983; and low (27 ± 2), 1984 to 1988. Wolf numbers declined ( P = 0.0001) 39 and 63% from 1970 to 1975 to 1976 to 1983 and from 1970 to 1975 to 1984 to 1988, respectively. Weight was similar between male and female pups and did not vary as wolf density changed. Mean hemoglobin ( P = 0.04), red ( P = 0.0001) and white blood cells ( P = 0.002), mean corpuscular volume, mean corpuscular hemoglobin concentration and mean corpuscular hemoglobin ( P = 0.0001) did differ among the multi-annual phases of changing wolf density. Weight and hematologic data also were compared to values from captive wolf pups. The high, but declining wolf density was associated with macrocytic, normochromic anemia in wolf pups, whereas the lowest density coincided with a hypochromic anemia. Although hematologic values show promise for assessing wolf pup condition and wolf population status, they must be used cautiously until data are available from other populations.

Minnesota↗

Was disease involved in the decimation of Guam's avifauna?

Between 1982 and 1986, 402 (290 live, 112 dead) exotic, migrant or native resident birds on Guam were surveyed for disease-causing agents to determine the role of disease in the decline of native forest bird populations on Guam. Traumatic injury, primarily from collisions with motor vehicles and predation, was the most prevalent (46%) cause of death. Thirty-eight percent of the carcasses examined were in poor body condition largely as a result of inadequate nutrition in captive native birds and poultry and adipose exhaustion in errant migrants. A variety of commensal or opportunistic bacteria, including Salmonella spp., were cultured from 220 birds, and nothing remarkable was found in 15 fecal samples. Lastly, no haematozoans, the suspected cause for the decline of the Hawaiian avifauna, were observed in blood slides examined from 260 birds. Based on the results of the survey and other lines of evidence presented in the discussion, we concluded there were no data implicating disease in the decline of Guam's avifauna.

Guam↗

Lead poisoning of spectacled eiders (Somateria fischeri) and of a common eider (Somateria mollissima) in Alaska

Lead poisoning was diagnosed in four spectacled eiders ( Somateria fischeri ) and one common eider ( Somateria mollissima ) found dead or moribund at the Yukon Delta National Wildlife Refuge, Alaska (USA) in 1992, 1993, and 1994. Ingested lead shot was found in the lower esophagus of one spectacled eider and in the gizzard of the common eider. Lead concentrations in the livers of the spectacled eiders were 26 to 38 ppm wet weight, and 52 ppm wet weight in the liver of the common eider. A blood sample collected from one of the spectacled eiders before it was euthanized had a lead concentration of 8.5 ppm wet weight. This is the first known report of lead poisoning in the spectacled eider, recently listed as a threatened species by the U.S. Fish and Wildlife Service.

Alaska↗

Diagnostic criteria for selenium toxicosis in aquatic birds: dietary exposure, tissue concentrations, and macroscopic effects

A feeding study with mallard ducks ( Anas platyrhynchos ) was conducted during March to July 1988 in Laurel, Maryland (USA), to identify diagnostic criteria for selenium toxicosis in birds. One-year-old male mallards in groups of 21 were fed diets containing 0, 10, 20, 40, or 80 parts per million (ppm) selenium, as seleno-DL-methionine, for 16 weeks. All ducks receiving 80 ppm died. Ducks receiving 40 or 80 ppm selenium consumed less feed than ducks in the other treatment groups. Body weights of ducks receiving 40 or 80 ppm selenium declined during the study. The post-breeding molt was delayed in ducks receiving 40 ppm; most ducks receiving 80 ppm selenium died prior to the onset of molt. At necropsy, numerous abnormalities were observed in ducks that died but only a small number of abnormalities were observed in ducks surviving to the end of the study in the 40 ppm group. Weights of the heart, spleen, and pancreas were mostly lower and weights of the kidney were higher for ducks dying during the study than for euthanized ducks. Liver weights were unaffected. Selenium accumulated in soft tissues approximately in proportion to dietary concentrations. Selenium concentrations in tissues of all ducks that died were different from those of surviving ducks in the 0, 10, and 20 ppm groups, but were not different from those of surviving ducks in the 40 ppm group. Proposed diagnostic criteria for fatal chronic selenosis were derived from body weight, macroscopic abnormalities, organ weights, and concentrations of selenium in the liver. Proposed diagnostic criteria for non-fatal chronic selenosis were derived from body weight, plumage condition, macroscopic abnormalities, concentrations of selenium in the liver, reproductive failure, and alterations of blood and tissue chemistries. Lead or dioxin poisoning have diagnostic criteria most similar to selenium toxicosis.

Journal of Wildlife Diseases↗

Sensitivity of condition indices to changing density in a white-tailed deer population

The ways in which comprehensive condition profiles, incorporating morphometric, histologic, physiologic, and diet quality indices, responded to changes in density of a white-tailed deer ( Odocoileus virginianus ) population were examined. Changes in these condition indices were monitored in a northeastern Oklahoma deer herd as density declined from peaks of 80 and 72 deer/km 2 in 1989 and 1990 (high-density) to lows of 39 and 41 deer/km 2 in 1991 and 1992 (reduced-density), respectively. Compared to a reference population (6 deer/km 2 ), deer sampled during high-density exhibited classic signs of nutritional stress such as low body and visceral organ masses (except elevated adrenal gland mass), low fecal nitrogen levels, reduced concentrations of serum albumin, elevated serum creatinine concentrations, and a high prevalence of parasitic infections. Although density declined by one half over the 4-yr study, gross indices of condition (in particular body mass and size) remained largely unchanged. However, selected organ masses, serum albumin and non-protein nitrogen constituents, and fecal nitrogen indices reflected improvements in nutritional status with reductions in density. Many commonly used indices of deer condition (fat reserves, hematocrit, total serum protein, and blood urea nitrogen) were not responsive to fluctuations in density.

Journal of Wildlife Diseases↗