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At least 775 records · Page 43Linked to original sources

Testing environmental DNA from wolf snow tracks for species, sex, and individual identification

Monitoring elusive, relatively low-density, large predators, such as the grey wolf (Canis lupus), has often been accomplished by live-capture and radiocollaring. Increasingly, non-invasive methods are considered best practice whenever it is possible to use them. Recently, environmental DNA (eDNA) deposited in snow tracks was demonstrated as useful for identifying lynx (Lynx canadensis), fisher (Pekania pennanti), wolverine (Gulo gulo), sika deer (Cervus nippon), red fox (Vulpes vulpes), and the Japanese marten (Martes melampus) to species level using mitochondrial DNA (mtDNA) markers. We tested whether eDNA from fresh wolf snow tracks collected in the Superior National Forest, Minnesota, USA during winter 2019 could be used to identify species, sex, and individual. Seven of the 8 snow track samples were successfully identified to “wolf-dog” species using mtDNA, with alleles amplifying in 5 of the samples at 1 or both of loci u250 and FH2096 in the allele range for wolves for this population. None yielded enough high-quality DNA to obtain genotypes to determine individual or sex. We recommend additional field trials to determine the minimum number of tracks required per individual to obtain sufficient, high-quality eDNA, as well as collecting associated urine or blood (from estrus) when possible. If individual wolves could be identified and sexed by the eDNA in their snow tracks, researchers should be able to determine population and family or group metrics with greater precision and less effort than typically required when conducting winter scat or hair-based genetic field studies of low density, elusive carnivores. Furthermore, this method would have applications to other areas of research and management, such as hunting quota determinations, validation of field methods and, in particular regards to wolves and other predators, livestock depredation issues. Overall, this technique holds significant future promise as field and laboratory methods are further refined for greater precision and optimized regarding the varying collection, filtration, and extraction protocols for different species in various environments.

Minnesota↗

Evaluating ichthyoplankton sampling as an effective method for early detection of novel aquatic invasive species in large bays of western Lake Erie

Early detection and monitoring are critically important for effective management of invasive species in the Laurentian Great Lakes. Specifically, Lake Erie is at high-risk for aquatic invasive species introductions due to many factors such as shoreline development, warm water temperatures, and transoceanic shipping traffic. Rare species captured are often used as a surrogate to assess sampling effectiveness for new or rare invasive species in vulnerable areas, such as Maumee and Sandusky bays of western Lake Erie. We compared species catches from multiple larval fish collection methods to those from adult and juvenile fish sampling. The specific objectives of this study were to: 1) evaluate the effectiveness of larval fish sampling for aquatic invasive species early detection in two bays of western Lake Erie: Maumee Bay and Sandusky Bay; 2) evaluate the effectiveness of passive and active sampling techniques at capturing different larval fish taxa; and 3) compare effectiveness of larval sampling techniques to juvenile and adult fish sampling methods for detecting new and rare species. While no new non-native species were captured during the study, we found that larval fish sampling techniques were not effective for detecting new or rare species but could be used with other life-stage sampling. Active sampling with bongo nets was more effective than light trap sampling, capturing a higher number of individuals and taxa. Juvenile and adult fish sampling methods provided higher precision in fish identification without the use of genetic tools. Because of the finer taxonomic resolution possible when identifying juvenile and adult fishes, sampling for adult and juveniles was found to be more robust and reliable for early detection of new and rare species compared to larval fish sampling. However, larval fish sampling can still be appropriate depending on study goals and objectives or as emerging technologies allow for greater taxonomic resolution of sampled fish.

Michigan, Ohio↗

Computing discharge using the index velocity method

Application of the index velocity method for computing continuous records of discharge has become increasingly common, especially since the introduction of low-cost acoustic Doppler velocity meters (ADVMs) in 1997. Presently (2011), the index velocity method is being used to compute discharge records for approximately 470 gaging stations operated and maintained by the U.S. Geological Survey. The purpose of this report is to document and describe techniques for computing discharge records using the index velocity method. Computing discharge using the index velocity method differs from the traditional stage-discharge method by separating velocity and area into two ratings—the index velocity rating and the stage-area rating. The outputs from each of these ratings, mean channel velocity (V) and cross-sectional area (A), are then multiplied together to compute a discharge. For the index velocity method, V is a function of such parameters as streamwise velocity, stage, cross-stream velocity, and velocity head, and A is a function of stage and cross-section shape. The index velocity method can be used at locations where stage-discharge methods are used, but it is especially appropriate when more than one specific discharge can be measured for a specific stage. After the ADVM is selected, installed, and configured, the stage-area rating and the index velocity rating must be developed. A standard cross section is identified and surveyed in order to develop the stage-area rating. The standard cross section should be surveyed every year for the first 3 years of operation and thereafter at a lesser frequency, depending on the susceptibility of the cross section to change. Periodic measurements of discharge are used to calibrate and validate the index rating for the range of conditions experienced at the gaging station. Data from discharge measurements, ADVMs, and stage sensors are compiled for index-rating analysis. Index ratings are developed by means of regression techniques in which the mean cross-sectional velocity for the standard section is related to the measured index velocity. Most ratings are simple-linear regressions, but more complex ratings may be necessary in some cases. Once the rating is established, validation measurements should be made periodically. Over time, validation measurements may provide additional definition to the rating or result in the creation of a new rating. The computation of discharge is the last step in the index velocity method, and in some ways it is the most straight-forward step. This step differs little from the steps used to compute discharge records for stage-discharge gaging stations. The ratings are entered into database software used for records computation, and continuous records of discharge are computed.

Techniques and Methods↗

Determination of nanogram per liter concentrations of volatile organic compounds in water by capillary gas chromatography and selected ion monitoring mass spectrometry and its use to define groundwater flow directions in Edwards Aquifer, Texas

A method has been developed to measure nanogram per liter amounts of selected volatile organic compounds (VOCs) including dichlorodifluoromethane, trichlorofluoromethane, cis-1,2-dichloroethene, trichloroethene, tetrachloroethene, and the isomers of dichlorobenzene in water. The method uses purge-and-trap techniques on a 100 mL sample, gas chromatography with a megabore capillary column, and electron impact, selected ion monitoring mass spectrometry. Minimum detection levels for these compounds ranged from 1 to 4 ng/L in water. Recoveries from organic-free distilled water and natural groundwater ranged from 70.5% for dichlorodifluoromethane to 107.8% for 1,4-dichlorobenzene. Precision was generally best for cis-1,2-dichloroethene, tetrachloroethene, and the dichlorobenzene isomers and worst for dichlorodifluoromethane and trichlorofluoromethane. Blank data indicated persistent, trace-level introduction of dichlorodifluoromethane, 1,4-dichlorobenzene, and tetrachloroemene to samples during storage and shipment at concentrations less than the method reporting limits. The largest concentrations of the selected VOCs in 27 water samples from the Edwards aquifer near San Antonio, TX, were from confined-zone wells near an abandoned landfill. The results defined a zone of water with no detectable VOCs in nearly all of the aquifer west of San Antonio and from part of the confined zone beneath San Antonio.

Analytical Chemistry↗

Measurement of fundamental illite particle thicknesses by X-ray diffraction using PVP-10 intercalation

The thicknesses of fundamental illite particles that compose mixed-layer illite-smectite (I-S) crystals can be measured by X-ray diffraction (XRD) peak broadening techniques (Bertaut-Warren-Averbach [BWA] method and integral peak-width method) if the effects of swelling and XRD background noise are eliminated from XRD patterns of the clays. Swelling is eliminated by intercalating Na-saturated I-S with polyvinylpyrrolidone having a molecular weight of 10,000 (PVP-10). Background is minimized by using polished metallic silicon wafers cut perpendicular to (100) as a substrate for XRD specimens, and by using a single-crystal monochromator. XRD measurements of PVP-intercalated diagenetic, hydrothermal and low-grade metamorphic I-S indicate that there are at least 2 types of crystallite thickness distribution shapes for illite fundamental particles, lognormal and asymptotic; that measurements of mean fundamental illite particle thicknesses made by various techniques (Bertant-Warren-Averbach, integral peak width, fixed cation content, and transmission electron microscopy [TEM]) give comparable results; and that strain (small differences in layer thicknesses) generally has a Gaussian distribution in the log-normal-type illites, but is often absent in the asymptotic-type illites.

Clays and Clay Minerals↗

Digital image measurement of the area and anatomical structure of tree rings

A microcomputer coupled to a video digitizer was used to develop methods for measuring areas of tree rings and for delineating and measuring microscopic anatomical features. Digitally imaged areas of individual rings of a loblolly pine cross section were within 2.6% of areas measured with a planimeter and were more accurate than areas calculated from radius widths. Various anatomical features were "mapped" within digitized images of consecutive rings, individual rings, and highly magnified parts of the rings of green ash. Fibers within various ring zones, including those known to have formed when trees were flooded, were readily differentiated on the basis of image brightness. The image-determined ratio of lumen to wall material within different groups of fibers was considered a measure of structural comparability. These techniques thus provide a rapid, accurate method to study relationships between tree rings and factors affecting their development. Methods could be improved by increasing both spatial and brightness resolution.

Canadian Journal of Botany↗

The role of water in unconventional in situ energy resource extraction technologies

Global trends toward developing new energy resources from lower grade, larger tonnage deposits that are not generally accessible using “conventional” extraction methods involve variations of subsurface in situ extraction techniques including in situ oil shale retorting , hydraulic fracturing of petroleum reservoirs, and in situ recovery of uranium. Although these methods are economically feasible and perhaps result in a smaller above-ground land-use footprint, there remain uncertainties regarding potential subsurface impacts to groundwater. This chapter provides an overview of the role of water in these technologies and the opportunities and challenges for water reuse and recycling.

Book chapter↗

Can reliable sage-grouse lek counts be obtained using aerial infrared technology

More effective methods for counting greater sage-grouse (Centrocercus urophasianus) are needed to better assess population trends through enumeration or location of new leks. We describe an aerial infrared technique for conducting sage-grouse lek counts and compare this method with conventional ground-based lek count methods. During the breeding period in 2010 and 2011, we surveyed leks from fixed-winged aircraft using cryogenically cooled mid-wave infrared cameras and surveyed the same leks on the same day from the ground following a standard lek count protocol. We did not detect significant differences in lek counts between surveying techniques. These findings suggest that using a cryogenically cooled mid-wave infrared camera from an aerial platform to conduct lek surveys is an effective alternative technique to conventional ground-based methods, but further research is needed. We discuss multiple advantages to aerial infrared surveys, including counting in remote areas, representing greater spatial variation, and increasing the number of counted leks per season. Aerial infrared lek counts may be a valuable wildlife management tool that releases time and resources for other conservation efforts. Opportunities exist for wildlife professionals to refine and apply aerial infrared techniques to wildlife monitoring programs because of the increasing reliability and affordability of this technology.

Nevada;Wyoming↗

Report of the Committee on Runoff, 1949–1950

The Weather Bureau activities in the runoff field during the 1949–1950 year centered largely around its flood and water supply forecasting activities. A new River Forecast Center was inaugurated at Portland, Oregon in January, 1950 to provide service for the Columbia River Basin and adjacent coastal areas. The first custom‐made model of the electronic flood routing machine has been delivered and is being tested to determine its electrical and operating characteristics. A large portion of the Bureau's investigations during the year were aimed at developing to the fullest all possible applications of existing techniques but considerable effort was devoted to the problem of developing new methods for short‐range forecasting of runoff from melting snow. A technique has been developed which appears to satisfy the requirements but it has not yet been adequately tested. Water supply forecasting activities were continued and the number of forecast points increased to about 300. The Cooperative Snow Investigations, with the Corps of Engineers, continued during the year.

Eos, Transactions, American Geophysical Union↗

Removing sun glint from optical remote sensing images of shallow rivers

Sun glint is the specular reflection of light from the water surface, which often causes unusually bright pixel values that can dominate fluvial remote sensing imagery and obscure the water-leaving radiance signal of interest for mapping bathymetry, bottom type, or water column optical characteristics. Although sun glint is ubiquitous in fluvial remote sensing imagery, river-specific methods for removing sun glint are not yet available. We show that existing sun glint-removal methods developed for multispectral images of marine shallow water environments over-correct shallow portions of fluvial remote sensing imagery resulting in regions of unreliable data along channel margins. We build on existing marine glint-removal methods to develop a river-specific technique that removes sun glint from shallow areas of the channel without overcorrection by accounting for non-negligible water-leaving near-infrared radiance. This new sun glint-removal method can improve the accuracy of spectrally-based depth retrieval in cases where sun glint dominates the at-sensor radiance. For an example image of the gravel-bed Snake River, Wyoming, USA, observed-vs.-predicted R 2 values for depth retrieval improved from 0.66 to 0.76 following sun glint removal. The methodology presented here is straightforward to implement and could be incorporated into image processing workflows for multispectral images that include a near-infrared band.

Earth Surface Processes and Landforms↗

Comparison of evaporation computation methods, Pretty Lake, Lagrange County, northeastern Indiana

Evaporation from Pretty Lake has been computed for a 2%- year period between 1963 and 1965 by the use of an energy budget, mass-transfer parameters, a water budget, a class-A pan, and a computed pan evaporation technique. The seasonal totals for the different methods are within 8 percent of their mean and are within 11 percent of the rate of 79 centimeters (31 inches) per year determined from published maps that are based on evaporation-pan data. Period-by-period differences among the methods are larger than the annual differences, but there is a general agreement among the evaporation hydrographs produced by the different computation methods. The energy budget was an excellent means of computing unbiased evaporation data for periods of a month or longer from June through September. It is not reliable in the springtime, when Bowen ratios are large and when the large changes in stored energy may be hard to measure accurately owing to errors in the capacity table. The need for sophisticated equipment, frequent temperature surveys, and complex computations makes the energy budget the most expensive of the several methods used. Effective use was made of the Koberg method in estimating long-wave radiation when accurate instrument records were not available. Effects of sediment heating and cooling were computed to have influenced evaporation as much as 0.03 cm day" 1 (centimeters per day) just after the autumnal overturn. The change is significant during the fall, when the evaporation for Pretty Lake is low, and would be more significant in a shallow lake, where the heat storage by the sediment would be large in proportion to the storage by the water. The corrected fall in stage computed by the water-budget method agreed well with the evaporation rates computed by other methods during the dryer seasons. Decreased rates of fall in stage during the wet seasons indicated net inflow seepage that was estimated to be equivalent to a stage change of more than 0.2 cm day" 1 at some times. Evaporation data based upon class-A pan records and computed pan evaporation were too large early in the season and too small late in the season. The differences were caused by energy storage, which affected the lake evaporation as energy was stored in the spring and released late in the season. Energy-storage effects can be corrected, but the corrections require some of the same expensive data that were used in the energy budget. The mass-transfer system proved to be an effective low-cost means of computing evaporation, a means that is well suited to low evaporation rates. The mass-transfer coefficient was determined to be 0.00560 cm hr day- 1 mile" 1 mb" 1 (centimeter per day per millibar per mile/hour), the relative standard error of the energy-budget calibration being about 6 percent. Springtime and autumn evaporation rates computed by the mass-transfer method were slightly higher than rates computed by other methods, and summer rates from mass-transfer computations were slightly lower than rates computed by other methods. Anemometer stalling is believed to have caused unreliable mass-transfer evaporation data during two periods having very low wind velocities. Assuming that Pretty Lake is typical of the many small natural lakes in its region, it is concluded that in most cases the evaporation information needed for hydrologic studies can be provided with satisfactory accuracy by a combination of the mass-transfer method and one or two other methods, without the expense of a complex energy-budget study. The different methods, although poor, agree that evaporation when there is ice cover is generally small (less than 0.1 cm day" 1 ), but the evaporation rates during the few days just before freezeup or just after ice breakup are significant

Indiana↗

An automated cross-correlation based event detection technique and its application to surface passive data set

In studies on heavy oil, shale reservoirs, tight gas and enhanced geothermal systems, the use of surface passive seismic data to monitor induced microseismicity due to the fluid flow in the subsurface is becoming more common. However, in most studies passive seismic records contain days and months of data and manually analysing the data can be expensive and inaccurate. Moreover, in the presence of noise, detecting the arrival of weak microseismic events becomes challenging. Hence, the use of an automated, accurate and computationally fast technique for event detection in passive seismic data is essential. The conventional automatic event identification algorithm computes a running-window energy ratio of the short-term average to the long-term average of the passive seismic data for each trace. We show that for the common case of a low signal-to-noise ratio in surface passive records, the conventional method is not sufficiently effective at event identification. Here, we extend the conventional algorithm by introducing a technique that is based on the cross-correlation of the energy ratios computed by the conventional method. With our technique we can measure the similarities amongst the computed energy ratios at different traces. Our approach is successful at improving the detectability of events with a low signal-to-noise ratio that are not detectable with the conventional algorithm. Also, our algorithm has the advantage to identify if an event is common to all stations (a regional event) or to a limited number of stations (a local event). We provide examples of applying our technique to synthetic data and a field surface passive data set recorded at a geothermal site.

Geophysical Prospecting↗

Using a modified time-reverse imaging technique to locate low-frequency earthquakes on the San Andreas Fault near Cholame, California

We present a new method to locate low-frequency earthquakes (LFEs) within tectonic tremor episodes based on time-reverse imaging techniques. The modified time-reverse imaging technique presented here is the first method that locates individual LFEs within tremor episodes within 5 km uncertainty without relying on high-amplitude P-wave arrivals and that produces similar hypocentral locations to methods that locate events by stacking hundreds of LFEs without having to assume event co-location. In contrast to classic time-reverse imaging algorithms, we implement a modification to the method that searches for phase coherence over a short time period rather than identifying the maximum amplitude of a superpositioned wavefield. The method is independent of amplitude and can help constrain event origin time. The method uses individual LFE origin times, but does not rely on a priori information on LFE templates and families.We apply the method to locate 34 individual LFEs within tremor episodes that occur between 2010 and 2011 on the San Andreas Fault, near Cholame, California. Individual LFE location accuracies range from 2.6 to 5 km horizontally and 4.8 km vertically. Other methods that have been able to locate individual LFEs with accuracy of less than 5 km have mainly used large-amplitude events where a P-phase arrival can be identified. The method described here has the potential to locate a larger number of individual low-amplitude events with only the S-phase arrival. Location accuracy is controlled by the velocity model resolution and the wavelength of the dominant energy of the signal. Location results are also dependent on the number of stations used and are negligibly correlated with other factors such as the maximum gap in azimuthal coverage, source–station distance and signal-to-noise ratio.

California↗

Characterization of Metarhizium species and varieties based on molecular analysis, heat tolerance and cold activity

Aims: The genetic relationships and conidial tolerances to high and low temperatures were determined for isolates of several Metarhizium species and varieties. Methods and Results: Molecular-based techniques [AFLP and rDNA (ITS1, ITS2 and 5??8S) gene sequencing] were used to characterize morphologically identified Metarhizium spp. isolates from a wide range of sources. Conidial suspensions of isolates were exposed to wet heat (45 ?? 0??2??C) and plated on potato dextrose agar plus yeast extract (PDAY) medium. After 8-h exposure, the isolates divided clearly into two groups: (i) all isolates of Metarhizium anisopliae var. anisopliae (Ma-an) and Metarhizium from the flavoviride complex (Mf) had virtually zero conidial relative germination (RG), (ii) Metarhizium anisopliae var. acridum (Ma-ac) isolates demonstrated high heat tolerance (c. 70-100% RG). Conidial suspensions also were plated on PDAY and incubated at 5??C for 15 days, during which time RGs for Ma-an and Ma-ac isolates were virtually zero, whereas the two Mf were highly cold active (100% RG). Conclusions: Heat and cold exposures can be used as rapid tools to tentatively identify some important Metarhizium species and varieties. Significance and Impact of the Study: Identification of Metarhizium spp. currently relies primarily on DNA-based methods; we suggest a simple temperature-based screen to quickly obtain tentative identification of isolates as to species or species complexes. ?? 2009 The Society for Applied Microbiology.

Journal of Applied Microbiology↗

An improved ion-selective electrode method for the rapid determination of fluorine in rocks and soil

An improved method based on an ion-selective electrode technique for the analysis of fluorine in rocks and soils is presented. Analyses are made by using a sodium carbonate-potassium carbonate fusion and a citric acid dissolution of the fuseate. Prior to determining the fluorine concentration by a standard-addition procedure, sodium citrate buffer is added to the solution. The proposed method yields fluorine values in agreement with known values for standard rocks. Values of this method are also presented for six geochemical exploration rock-and-soil reference samples and compared to those from other ion-selective electrode techniques. Fifty samples can be analyzed in 1 person-day. The sensitivity of the method is 100 parts per million, and samples containing up to 45 percent fluorine have been successfully analyzed.

Journal of Research of the U.S. Geological Survey↗

Models and methods to characterize site amplification from a pair of records

The paper presents a tutorial review of the models and methods that are used to characterize site amplification from the pairs of rock- and soil-site records, and introduces some new techniques with better theoretical foundations. The models and methods discussed include spectral and cross-spectral ratios, spectral ratios for downhole records, response spectral ratios, constant amplification factors, parametric models, physical models, and time-varying filters. An extensive analytical and numerical error analysis of spectral and cross-spectral ratios shows that probabilistically cross-spectral ratios give more reliable estimates of site amplification. Spectral ratios should not be used to determine site amplification from downhole-surface recording pairs because of the feedback in the downhole sensor. Response spectral ratios are appropriate for low frequencies, but overestimate the amplification at high frequencies. The best method to be used depends on how much precision is required in the estimates.

Earthquake Spectra↗

Screening fungicides for use in fish culture: Evaluation of the agar plug transfer, cellophane transfer, and agar dilution methods

The reliability, reproducibility, and usefulness of three screening methods -- the cellophane transfer, the agar plug transfer, and the agar dilution -- to screen aquatic fungicides were evaluated. Achlya flagellata and Saprolegnia hypogyna were exposed to 1, 10, and 100 mg/L of malachite green to test each method. The cellophane transfer and agar plug transfer techniques had similar reliability and reproducibility in rating fungicidal activity, and were both superior to the agar dilution technique. The agar plug transfer and agar dilution techniques adequately projected in vivo activity of malachite green, but the cellophane transfer technique overestimated its activity. Overall, the agar plug transfer technique most accurately rated the activity of malachite green and was the easiest test to perform. It therefore appears to be the method of choice for testing aquatic fungicides.

Progressive Fish-Culturist↗

Using the Lomb-Scargle method for wave statistics from gappy time series

Sandwich Town Neck Beach in Sandwich, MA, has experienced substantial erosion and has been the subject of efforts by the town and private landowners to limit the sand loss. Erosion has been particularly dramatic in the past five years with the loss of dwellings. Sandwich's nourishment efforts presented a unique opportunity for scientists at the U.S. Geological Survey Woods Hole Coastal and Marine Science Center to monitor beach morphology and to test new technologies and techniques such as geo-referenced drone imaging. Two bottom lander deployments were performed in Cape Cod Bay at a location that was key to model the fate of waves at Sandwich Town Neck Beach and to support the study of beach morphological evolution. The study period was after the town nourished the beach and during a time when several intense winter storms reshaped the beach and removed much of the nourished sand. A TRDI Workhorse Sentinel V ADCP was used for both deployments. For wave bursts, the instruments collected 2048 samples at 2 Hz every hour. The first deployment during the winter of 2016 returned good quality data. The second deployment during the following winter had gaps throughout the time series from a wiring problem in the external battery pack. The timing of the gaps was random, the duration approximately 100 s. While most of the bursts started at the top of each hour, many had 1-3 gaps within. Time series data with random gaps are problematic for computing spectral density, and thus, wave statistics. This kind of situation is familiar in other scientific disciplines such as astrophysics [1], where techniques exist to find stationary signals in sparse data. One of these methods is the Lomb-Scargle technique for computing periodograms. The most useful feature of the Lomb-Scargle (LS) method is that it allows the spectral analysis of incomplete records, without having to manipulate the record to extrapolate from or replace missing data. We compared the effectiveness of LS against common methods of averaging Fourier transforms such as a simple un-windowed Fast Fourier transform (FFT), Welch's method, and TRDI's Wavesmon software; methods that are commonly used in oceanography for non-gappy data. Synthetic data series that have been artificially modified to introduce gaps were used to evaluate the performance of each method. The LS approach was able to recover spectral density even with several 100-s gaps present. The method was applied here to the gappy and non-gappy data from both Sandwich deployments, and wave statistics were obtained and compared to the wave-buoy data. LS was used to process data that contains gaps that was rejected by Wavesmon, which was approximately 39% of the dataset. Significant wave height and peak period from LS compared well with buoy data. Mean period computed on gappy data using LS produced values biased low, compared with other methods when gaps were filled with the mean value. The LS technique has potential to uncover low-frequency signals such as infragravity waves from gappy records where the non-gappy segments are not long enough to resolve them. It has potential to unlock new information from older data sets.

Massachusetts↗